Journal of lasers in medical sciences最新文献

{"title":"Therapeutic Effects of Low-Level Laser Therapy on Rat Spinal Cord Injury: Analysis of Inflammatory Markers and Testicular Function.","authors":"Hadise Taheri, Hamid Reza Mosleh, Shima Jahanbaz, Abbas Aliaghaei, Foozhan Tahmasebinia, Marzieh Abedi, Sama Abbasi, Hojjat-Allah Abbaszadeh","doi":"10.34172/jlms.2025.06","DOIUrl":"https://doi.org/10.34172/jlms.2025.06","url":null,"abstract":"<p><p><b>Introduction:</b> Spinal cord injury (SCI) is a severe condition often leading to lasting neurological impairment and associated reproductive health issues in males. The aim of this study was to investigate the therapeutic potential of photobiomodulation therapy (PBMT) during the inflammatory phase of SCI to prevent oxidative damage, reduce inflammation, and mitigate potential damage to testicular function. <b>Methods:</b> Eighteen male rats were randomly divided into three groups: Group A (laminectomy only), group B (contusion), and group C (contusion+PBMT). Thirty minutes post-injury, group C received PBMT for two weeks. Histological and stereological analysis was evaluated using the hematoxylin and eosin test (H&E). ELISA and real-time PCR were performed for eight weeks post-SCI to assess testosterone and inflammatory and apoptotic changes. Statistical analysis was performed using SPSS with one-way ANOVA and Tukey's post hoc test, and statistical significance was set at <i>P</i><0.05. <b>Results:</b> The SCI group exhibited significant reductions in sperm count (mean±SD: 14.2±86.1), motility (34.8±72.8), and viability (26.12±43.9), with increased levels of inflammatory markers (IL-1 β: 4.4±71.6, TNF-α: 3.14±66) and damage to testicular structure. In contrast, animals treated with PBMT showed significant improvements in sperm parameters (sperm count: 48±34, motility: 57.2±18.5, viability: 52.3±88.2) and a marked reduction in inflammation (IL-1 β: 3.09±14.2, TNF-α: 2.67±74.1) compared to untreated SCI animals (<i>P</i><0.001). Additionally, PBMT-treated animals demonstrated significant improvements in testosterone levels (1.57±44.8) and a reversal of testicular cell loss (<i>P</i><0.001). <b>Conclusion:</b> These findings suggest that PBMT mitigates the negative effects of SCI on testicular tissue by reducing inflammation and preserving cellular integrity, thus supporting its use as a dual therapeutic approach to aid neurological recovery and maintain reproductive health.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"16 ","pages":"e6"},"PeriodicalIF":1.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Blood Products and Photobiomodulation Therapy for Neurosensory Recovery in Patients With Inferior Alveolar Nerve Injury Following Orthognathic Surgery: A Systematic Review Study.","authors":"Maryam Nasiri, Akram Ghannadpour, Neda Hakimiha","doi":"10.34172/jlms.2025.05","DOIUrl":"https://doi.org/10.34172/jlms.2025.05","url":null,"abstract":"<p><p><b>Introduction:</b> Inferior alveolar nerve (IAN) injury is among the most common complications associated with orthognathic surgery. Managing these injuries poses significant challenges due to the lack of a standardized treatment protocol. This study aimed to systematically review the literature on the efficacy of blood products and photobiomodulation (PBM) therapy for neurosensory recovery in patients with IAN injuries after orthognathic surgery. <b>Methods:</b> This systematic review involved a comprehensive search of Scopus, Embase, and PubMed databases, as well as the initial 100 search results from Google Scholar, to identify relevant articles published between 2015 and 2024. The articles were selected using defined eligibility criteria. <b>Results:</b> The research paper reviewed 170 articles, ultimately including 14 studies that focused on IAN injury related to surgical procedures such as genioplasty, sagittal split mandibular ramus osteotomy (SSRO), and bilateral sagittal split osteotomy (BSSO). Among these studies, five assessed the effectiveness of various blood products-specifically platelet-rich fibrin (PRF), leukocyte-rich PRF (L-PRF), advanced PRF (A-PRF), and concentrated growth factor (CGF)-all of which were found to alleviate hypoesthesia. Nine studies evaluated the efficacy of PBM, primarily utilizing wavelengths of 810 and 808 nm over 5 to 10 sessions. PBM also successfully enhanced the recovery of IAN. Moreover, one article highlighted the synergistic effect of using L-PRF in conjunction with PBM. <b>Conclusion:</b> It appears that neurosensory recovery following IAN injury due to orthognathic surgery may be enhanced by blood-derived products such as PRF, CGF, A-PRF, and L-PRF, which release growth factors that facilitate tissue repair. Additionally, PBM further supports recovery by reducing inflammation in the initial weeks and stimulating cellular metabolism to promote regeneration in the subsequent weeks.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"16 ","pages":"e5"},"PeriodicalIF":1.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Efficacy of Ultrasound and Laser Therapy in Modulating Inflammatory Markers in a Rat Model of Testicular Inflammation.","authors":"Ahmed Mamdouh Abdelkader, Ahmed Ali Torad, Mohamed Ahmed Elbedewy, Nihal Mostafa, Amr Saadeldeen Mohamed Shalaby, Tayseer Saber Abdeldayem, Alaa Anwar Ahmed","doi":"10.34172/jlms.2025.04","DOIUrl":"https://doi.org/10.34172/jlms.2025.04","url":null,"abstract":"<p><p><b>Introduction:</b> All three degrees of inflammation (cellular, tissue, and systemic) are normal reactions that our bodies have to foreign substances. Cellular damage from bacteria, viruses, and even cancer can be warded off with the aid of these reactions. An efficient non-pharmaceutical method of treating inflammation is frequently seen as physiotherapy. In this study, the effects of ultrasonic treatment (US) and low-level laser therapy (LLLT) on the levels of C-reactive protein (CRP) in rats with inflamed testicles are investigated. <b>Methods:</b> Three groups, each including eighteen male rats, were established: the control group (C), the US group (which received ultrasonic treatment), and the LLLT group (which received low-level laser therapy). <b>Results:</b> The blood CRP levels in the US group were significantly lower than those in the control and LLLT groups. <b>Conclusion:</b> Findings suggest that ultrasound might be more effective than LLLT in treating testicular inflammation, as shown by the decrease in blood CRP levels.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"16 ","pages":"e4"},"PeriodicalIF":1.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of GLUMA Desensitizer and Low-Level 980 nm Diode Laser on the Retention of the Metal Frames of the PFM Crowns Cemented by Self-adhesive Resin Cement (RelyX U200).","authors":"Mohammad Joudaki, Arash Azizi, Sahel Bazarnoei, Shirin Lawaf","doi":"10.34172/jlms.2025.03","DOIUrl":"https://doi.org/10.34172/jlms.2025.03","url":null,"abstract":"<p><p><b>Introduction:</b> Sometimes, dentinal tubules are exposed to the oral cavity during the preparation of vital teeth for crowns, and patients show various signs of tooth hypersensitivity. Some treatments have been suggested for dental hypersensitivity after tooth preparation, such as low-level diode laser (980 nm) and GLUMA desensitizer. The pretreatment of dentin with desensitizers may affect retention. The aim of this study was to ascertain the effects of the 980nm Diode laser and GLUMA Desensitizer on the retention of the metal frames cemented by self-adhesive resin cement (RelyX U200). <b>Methods:</b> Thirty intact permanent maxillary first premolars were used in this study. After preparing teeth with 0.5 to 0.7 finishing line above CEJ, casting full metal crowns with a 5-diameter ring on the occlusal surface was done. Then the samples were divided into 3 groups: (1) Control Group (n=10), (2) 980 nm diode laser (n=10), and (3) GLUMA desensitizer (n=10). Frames were cemented with self-adhesive resin cement (RelyX U200) and the bond strength was measured by universal testing machine. Data were analyzed by ANOVA and Tukey post hoc tests in SPSS version 25 (<i>P</i><0.05). <b>Results:</b> Frame retention in the 980 nm diode laser group (40.90±27.07N) was significantly lower than the control group (138.17±40.81 N) and the GLUMA desensitizer group (318.59±56.31N) (<i>P</i><0.05), and frame retention in the GLUMA Desensitizer group was significantly higher than the other groups (<i>P</i><0.05). <b>Conclusion:</b> The GLUMA desensitizer has a positive effect and the 980 nm diode laser has a negative effect on the retention of frames cemented by self-adhesive resin cement (RelyX U200).</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"16 ","pages":"e3"},"PeriodicalIF":1.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation Therapy in Improving Quality of Life in Kindler Syndrome: A Case Study.","authors":"Farha Khatoon, Sunil Surendraprasad Mishra, Vaishnavi Shailesh Sawant, Anupama Anand Chavan","doi":"10.34172/jlms.2025.02","DOIUrl":"https://doi.org/10.34172/jlms.2025.02","url":null,"abstract":"<p><p><b>Introduction:</b> Kindler syndrome (KS) is a rare genetic disorder characterized by skin fragility, acral blister formation, diffuse cutaneous atrophy, photosensitivity, palmoplantar hyperkeratosis, pseudosyndactyly, and alopecia. Oral manifestations include limited mouth opening, which adversely affects oral hygiene, chewing, and swallowing, significantly impacting the quality of life of affected individuals. <b>Methods:</b> A 26-year-old male with KS was referred for the management of reduced mouth opening, presenting with an interincisal distance of 19 mm. This was a critical concern for facilitating prosthetic rehabilitation. The patient underwent Photobiomodulation (PBM) therapy as a non-invasive treatment modality aimed at improving trismus. <b>Results:</b> Following PBM therapy, the patient's mouth opening improved significantly from 19 mm to 23 mm. This enhancement allowed for the initiation of prosthetic rehabilitation. <b>Conclusion:</b> PBM therapy is a promising treatment for managing trismus in KS by breaking down abnormal collagen deposits and reducing collagen synthesis, effectively addressing the underlying pathology. It offers significant improvements in mouth opening, which can enhance oral hygiene, nutritional intake, and overall quality of life. This non-invasive approach provides a viable option for patients with KS who require prosthetic rehabilitation.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"16 ","pages":"e2"},"PeriodicalIF":1.4,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Effect of 810 nm Diode Laser and Transcranial Direct Current Stimulation on Relieving Symptoms of Patients With Temporomandibular Joint Disorders.","authors":"Soghra Yassaei, Parisa Mohsenzade, MohammadHassan Akhavan Karbassi, Zahra Fazaelipour","doi":"10.34172/jlms.2025.01","DOIUrl":"https://doi.org/10.34172/jlms.2025.01","url":null,"abstract":"<p><p><b>Introduction:</b> The high prevalence of temporomandibular joint disorder (TMD) and the side effects of drug treatments, as well as invasive surgical methods, highlight the importance of less invasive and less complicated methods. This study aimed to compare the effectiveness of an 810 nm diode laser and transcranial direct current stimulation (TDCS) in the treatment of patients with TMD. <b>Methods:</b> The design of the study was a randomized controlled trial in which 34 patients with TMD were included and randomly treated with 810 nm diode laser or TDCS. In the laser group, 9 sessions of laser therapy were performed during 3 weeks. In the TDCS group, treatment was accomplished in 5 sessions within 5 consecutive days after sample selection. Before the first and after the last treatment session in both groups, the outcomes including the TMJ spontaneous pain score, facial muscle tenderness, maximum range of pain-free opening of the mouth and presence of jaw sounds were evaluated. The patient's satisfaction score was evaluated immediately after treatment, as well as one month later. The data were entered into SPSS statistical software version 17 and statistically analyzed by independent and paired t-tests. A significance level of less than 5% was considered. <b>Results:</b> The average TMJ spontaneous pain score and muscle tenderness score in temporal, TMJ, and gonial regions decreased significantly in both groups (<i>P</i><0.001), also the mean maximum mouth opening increased significantly in both groups (<i>P</i><0.05), but there was no significant difference between the two groups. The average score of patient satisfaction with the treatment immediately and one month after the treatment in the laser group was significantly higher than that of TDCS. <b>Conclusion:</b> 810 nm diode laser and TDCS were effective in reducing the symptoms of TMD patients. The level of satisfaction with the treatment in the laser group was significantly higher than that in the TDCS method.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"16 ","pages":"e1"},"PeriodicalIF":1.4,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12049580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144028078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Photodynamic Therapy With Two Different Photosensitizers on the Viability of Human Dental Pulp Stem Cells.","authors":"Shiva Shojaeian, Mohamad Asnaashari, Arash Heidari, Mahsa Sadeghi, Pegah Mehrabinia","doi":"10.34172/jlms.2024.70","DOIUrl":"10.34172/jlms.2024.70","url":null,"abstract":"<p><p><b>Introduction:</b> Photodynamic therapy(PDT)is a minimally invasive technique increasingly used in dentistry for its antimicrobial properties. This research aimed to evaluate the influence of photodynamic therapy (PDT) on the viability of dental pulp stem cells (DPSCs). <b>Methods:</b> In this laboratory-based, experimental study, DPSCs were cultured in Dulbecco's modified Eagle's medium and maintained at 37 °C. The cells were separated into five groups: Toluidine blue (TBO) at concentration of 0.1 mg/mL and 0.5 mg/mL, as well as methylene blue (MB) at concentrations of 0.01 mg/mL and 0.05 mg/mL were added to the wells in groups 1 to 4. The fifth group served as the control group. After 5 minutes of incubation, the experimental groups were irradiated with Fotosan® light-emitting diode (LED) for one minute. Cell viability was assessed after 8, 24, 48, and 72 hours using the methyl thiazolyl tetrazolium (MTT) assay. <b>Results:</b> Time (<i>P</i><0.000), photosensitizer type/concentration (<i>P</i><0.0001), and their interaction effect (<i>P</i><0.000) on cell viability were all significant. Viability in both MB groups was considerably higher than that in the control group at 8 hours (<i>P</i><0.001). At 24 hours, no significant difference was observed between the experimental groups and the control (<i>P</i>>0.05). At 48 and 72 hours, cell viability in the TBO groups was markedly lower compared to the control group (<i>P</i><0.01). <b>Conclusion:</b> PDT with MB at the tested concentrations had no adverse effect on DPSCs even in the long- term (48 and 72 hours).</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e70"},"PeriodicalIF":1.4,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Diode Laser and Fluoride Varnish on Microhardness of enamel: An In Vitro Study.","authors":"Hanieh Najjaran, Fatemeh Safari, Sara Valizadeh, Mohammad Ali Keshvad, Mohammad Javad Kharazifard, Nasim Chiniforush, Sepideh Arab","doi":"10.34172/jlms.2024.69","DOIUrl":"10.34172/jlms.2024.69","url":null,"abstract":"<p><p><b>Introduction:</b> White spots are a common complication of orthodontic treatment. Several prevention methods such as fluoride therapy and laser irradiation have been proposed, but there is still no conclusive evidence. Therefore, this study aimed to evaluate the combined application of diode laser and fluoride varnish in preventing white spots. <b>Methods:</b> Thirty-nine sound human premolars were extracted for orthodontic purposes and randomly divided into three groups of thirteen teeth each (C: Control, FV: fluoride varnish (5% sodium fluoride), and FV+L: fluoride varnish+980 nm diode laser (2 W)). The freshly extracted teeth were disinfected in a 0.5% chloramine T solution and stored in saline at room temperature. Each tooth was coated with nail polish, leaving a 2×3 mm² window on the midbuccal. Surface treatments were applied, and primary Vickers microhardness was evaluated in the gingival sections. Then, the samples were immersed in alternating demineralizing (6 hours/day) and remineralizing (18 hours/day) solutions and incubated at 37 °C for 9 days to produce artificial caries. Afterward, the samples were immersed in a remineralizing solution for one day. Finally, secondary microhardness was measured. The data were analyzed using one-way ANOVA. <b>Results:</b> The primary microhardness was higher in groups C and FV than in group FV+L. The lowest secondary microhardness was recorded in group C, and there was no significant difference between the other two groups. The change in microhardness was greater in group C, while no difference was observed between the FV and FV+L groups. <b>Conclusion:</b> Within the limitations of this study, the combined application of laser irradiation and fluoride varnish may not improve white spot lesion (WSL) prevention compared to the use of fluoride varnish alone.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e69"},"PeriodicalIF":1.4,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Efficacy of Laser Versus Conventional Method in the Success of Circumferential Supracrestal Fiberotomy in Orthodontic Patients: A Systematic Review.","authors":"Farhad Sobouti, Alireza Kashiri, Sepideh Dadgar, Mehdi Aryana, Osama Eissa, Neda Hakimiha, Mohadeseh Heidari","doi":"10.34172/jlms.2024.68","DOIUrl":"10.34172/jlms.2024.68","url":null,"abstract":"<p><p><b>Introduction:</b> The relapse of rotated teeth is a significant concern in orthodontics, and circumferential supracrestal fiberotomy (CSF) presents a potential solution. Traditionally, CSF is performed using a surgical blade; however, employing a laser may reduce complications. This study aimed to systematically review the literature comparing laser-assisted CSF with conventional methods in orthodontic patients. <b>Methods:</b> A comprehensive search was conducted across the PubMed, Scopus, Web of Science, and Cochrane Library databases using a specific search strategy up to June 16, 2024. Data extraction was done in accordance with the defined inclusion and exclusion criteria. <b>Results:</b> Initially, a total of 293 articles were identified through the search process, and ultimately, four were selected for analysis based on specific inclusion and exclusion criteria. The studies involved various types of lasers including diode (810 to 940 nm), Er:YAG, and Er,Cr:YSGG lasers, and examined the effects of low-level laser therapy (LLLT) in conjunction with surgical interventions. Regarding relapse rates, two studies reported that both laser and surgical blade techniques demonstrated comparable efficacy, while adjunctive LLLT was effective in reducing relapse. In the other two studies, the use of laser therapy was found to be effective in decreasing relapse rates compared to the control group. Pain assessment results were inconclusive; one study reported reduced pain in the laser group, while another found no significant difference in pain levels between the laser and blade techniques. Furthermore, no significant differences were observed in periodontal pocket depth or gingival recession between the two methods. <b>Conclusion:</b> Within the limitations of the current systematic review, our findings indicate that there is no significant difference in relapse rates between laser and blade CSF procedures. Furthermore, our results suggest that the incorporation of photobiomodulation at higher doses is correlated with a reduction in relapse rates following fiberotomy procedures.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e68"},"PeriodicalIF":1.4,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Efficacy of Photodynamic Therapy Versus Cisplatin Application.","authors":"Babak Arjmand, Ayad Bahadorimonfared, Somayeh Jahani Sherafat, Hamideh Moravvej, Mitra Rezaei, Fatemeh Daneshimehr, Nastaran Asri, Masoumeh Farahani","doi":"10.34172/jlms.2024.67","DOIUrl":"10.34172/jlms.2024.67","url":null,"abstract":"<p><p><b>Introduction:</b> Photodynamic therapy (PDT) is a photochemical treatment that involves the use of light and photosensitizer. This method is applied as a therapeutic approach against several types of cancer. The main aim of this study is to compare the efficacy of PDT with that of cisplatin (a well-known chemotherapy agent) through protein-protein interaction (PPI) network analysis. <b>Methods:</b> Gene expression profiles of human melanoma A375 cells from the Gene Expression Omnibus (GEO) were selected for analysis via directed PPI network analysis. The significant differentially expressed genes (DEGs) were identified and assessed based on co-expression interactions. The critical DEGs were introduced by considering out-degree and in-degree values. <b>Results:</b> Two directed PPI networks for upregulated and downregulated DEGs were constructed. TP53 was identified as a critical upregulated gene in response to cisplatin in comparison with PDT. EGFR, PPARG, MMP9, PTGS2, FOXO1, and RUNX2 were highlighted as the crucial downregulated genes due to the effect of cisplatin on the gene expression of the treated cells. <b>Conclusion:</b> Cisplatin directly targets key cellular functions such as cell growth, differentiation, migration, and invasion. It seems that the combination of cisplatin and PDT is a suitable method for treating cancers because cisplatin targets the key genes responsible for cancer development, while PDT intensifies the effect of cisplatin and reduces its side effects.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e67"},"PeriodicalIF":1.4,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}