Journal of lasers in medical sciences最新文献

{"title":"Effect of a 940 nm Diode Laser and MTAD on the Decontamination of Root Canals With <i>Enterococcus faecalis</i>.","authors":"Alvaro Tapia Gálvez, Abel Teves Cordova, Oniel Elías Juarez Vilcapuma, Katherine Joselyn Atoche-Socola, Sergio Jiménez Sánchez","doi":"10.34172/jlms.2024.57","DOIUrl":"10.34172/jlms.2024.57","url":null,"abstract":"<p><p><b>Introduction:</b> In recent years, various methods such as lasers and different solutions have been investigated to eliminate microorganisms in the root canal. This investigation was aimed at comparing the bactericidal effect on <i>Enterococcus faecalis</i> between a diode laser with a wavelength of 940 nm and a mixture of a tetracycline isomer, an acid, and a detergent (MTAD). <b>Methods:</b> Forty single-rooted human lower premolar teeth were prepared for inoculation with <i>E. faecalis</i> and incubated for a period of 3 weeks. The 40 samples were randomly divided into 4 groups: the 940 nm diode laser group with irradiation through a 200 µm optical fiber with parameters of (1 W/CW,4 cycles, 20s interval); the MTAD group irrigated with 5 mL for 5 minutes; also, a positive group with 3% NaOCl and a negative group with 0.9% saline solution, both irrigated with 5 mL for 5 minutes. For statistical analysis, the Mann-Whitney U and Wilcoxon tests were used. <b>Results:</b> The 3% NaOCl positive control group had greater bacterial reduction after treatment (99.99%) compared to the 940nm diode laser (98.96%) and MTAD (99.34%) groups. Statistically, we found a significant difference between all groups regarding the reduction in the colony count (<i>P</i><0.05). <b>Conclusion:</b> It was found that there was a significant difference in the percentage of reduction for the bacterial colonies between all the groups evaluated, with the greatest antibacterial effectiveness observed with 3% sodium hypochlorite.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e57"},"PeriodicalIF":1.4,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Fractional CO2 Laser and Topical <i>Centella asiatica</i> Combination Therapy in Striae Distensae (Stretch Marks).","authors":"Yuli Kurniawati, Yulia Farida Yahya, Aryati Fadhila, M Rezi Rahmanda, Nyimas Nursyarifah, Nanda Safira Alisa","doi":"10.34172/jlms.2024.56","DOIUrl":"10.34172/jlms.2024.56","url":null,"abstract":"<p><p><b>Introduction:</b> Striae distensae (SD) are a skin disorder characterized by linear atrophic depression of the dermis due to stretching of the skin. There are various SD therapy modalities, namely topical therapy, peeling, microneedling, platelet-rich plasma, and laser. Until now, there has been no standard therapy for SD, but several therapeutic modalities can reduce clinical symptoms, so knowledge about the current management of SD is needed. <b>Methods:</b> The research method used an experimental research design with consecutive sampling technique in SD patients at the Dermatology, Venereology and Aesthetics clinic of Mohammad Hoesin Hospital, Palembang, during the research period. This study aimed to determine the effectiveness of combination therapy with fractional CO₂ laser and <i>Centella asiatica</i> in SD. <b>Results:</b> This study included 22 SD patients who met the inclusion criteria and received combination therapy with fractional CO₂ laser and <i>Centella asiatica</i>. The results of the analysis of differences in the Index of Striae Distensae Assessment (INA) score, Dermatology Life Quality Index (DLQI) score and VAS score revealed that there was a significant difference in the mean decrease in the INA score between the baseline and week 12 (<i>P</i>=0.014), as well as in the DLQI score between the baseline and week 12 (<i>P</i>=0.000). There was a significant difference in the mean VAS score between the baseline and week 4 (<i>P</i>=0.000). <b>Conclusion:</b> These findings indicate that the combination of fractional CO₂ laser and topical <i>Centella asiatica</i> effectively reduces the severity of SD, improves patients' quality of life, and decreases associated pain. Further studies could explore long-term effects and optimize treatment protocols for even better outcomes.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e56"},"PeriodicalIF":1.4,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Low-Level Laser Irradiation on the Proliferation of Human Chondrocytes: An In Vitro Study.","authors":"Fahimeh Anbari, Hamidreza Khalighi, Maryam Baharvand, Sahba Khosousi Sani, Mani Sharaki, Zahra Yadegari, Seyed Masoud Mojahedi Nasab, Mohammad Khosousi Sani","doi":"10.34172/jlms.2024.55","DOIUrl":"10.34172/jlms.2024.55","url":null,"abstract":"<p><p><b>Introduction:</b> Chondrocytes are the major cell in hyaline cartilage playing a crucial role in maintaining the mechanical resilience of the tissue. We assessed the effect of an 808nm diode laser on the proliferation of human chondrocytes. <b>Methods:</b> This study was conducted on human chondrocytes in vitro. The cells were divided into 5 cases and one control group. The cells were irradiated by low-level laser 808 nm, with energy levels of 1, 2, 3 J/cm² (0.2 W, for 5, 10, and 15 seconds), 4 J/cm² (0.3 W,13 s), and 5 J/cm² (0.4 W,12 s). The culture was incubated for 24 hours. The MTT assay was performed to determine the cell viability. After 72 hours of incubation, the procedure was reperformed to assess the effect of incubation duration. The cell viability in terms of incubation duration and irradiation parameters were investigated by a two-way ANOVA test. Pairwise comparisons were performed using the Bonferroni test. <b>Results:</b> In the 72-hour incubation group, cell viability in the group that received 5 J/cm² energy was significantly lower than that in the groups receiving 1 J/cm², 2 J/cm² and 4 J/cm² irradiation. The variables of time (<i>P</i>=0.001) and energy level (<i>P</i>=0.024) had significant effects on the cell viability of the samples. In the 24-hour incubation groups, no significant difference in cell viability was observed. <b>Conclusion:</b> The diode 808 nm Low-level laser irradiation (LLLI) at doses of 5 J/cm² and less did not show a significant increase in the proliferation of chondrocytes (regardless of incubation time). However, the best survival rate of chondrocytes was observed in the group of 4 J/cm² with 72-hour incubation.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e55"},"PeriodicalIF":1.4,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625388/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of the Orifice-Level Passive Ultrasonic Activation Technique in Removing Debris from Mandibular Molars: An Ex Vivo Study.","authors":"Iman Bolourchi, Nazanin Zargar, Kamyar Khosravi, Fatemeh Mashhadiabbas, Alireza Akbarzadeh Baghban, Leila Pourmousavi","doi":"10.34172/jlms.2024.54","DOIUrl":"10.34172/jlms.2024.54","url":null,"abstract":"<p><p><b>Introduction:</b> This study aimed to evaluate the efficacy of the orifice-level passive ultrasonic activation (OL-PUA) technique in removing debris from the mesial root canals of mandibular molars, besides comparing it with other approaches, including manual dynamic agitation (MDA), EndoActivator, and laser-activated irrigation (LAI). <b>Methods:</b> Ninety mesial roots of mandibular molars were prepared up to 25.06 using the Race rotary system, filled with 3% sodium hypochlorite (NaOCl), and then they were randomly assigned to 5 groups (n=15) based on the activation protocol: MDA, EndoActivator, OL-PUA, LAI (using a diode laser), and needle irrigation (no activation). Moreover, one group served as the negative control using normal saline as the final irrigant. The roots were decalcified and sectioned into coronal, middle, and apical cross-sections. After histological staining, the remaining debris was assessed with an optical microscope and measured in the canals and isthmuses using histomorphometric analysis. Statistical analyses were performed by using one-way ANOVA and Tukey's HSD post hoc test (<i>P</i>=0.05). <b>Results:</b> Although less residual debris was observed after OL-PUA/LAI than using the EndoActivator/MDA methods, no significant difference was found between the four activation techniques at any root third (<i>P</i>>0.05). The OL-PUA/LAI group harbored significantly less remaining debris than the needle-irrigated samples at all root thirds (<i>P</i><0.05). Debris accumulation increased from the coronal to the apical root thirds. <b>Conclusion:</b> No statistical difference was detected between the four activation techniques in terms of debris-removal performance, and none of them resulted in completely debris-free canals. Orifice-level PUA proved to be a promising approach with significant efficacy in removing debris compared to control groups.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e54"},"PeriodicalIF":1.4,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Effect of Photobiomodulation Therapy With Different Wavelengths of Diode Lasers on the Proliferation and Adhesion of Human Gingival Fibroblast Cells to a Collagen Membrane: An In Vitro Study.","authors":"Mohammad Reza Karimi, Shahrzad Abdollahi, Ardavan Etemadi, Neda Hakimiha","doi":"10.34172/jlms.2024.53","DOIUrl":"10.34172/jlms.2024.53","url":null,"abstract":"<p><p><b>Introduction:</b> Photobiomodulation (PBM) is considered a promising adjunctive approach in regenerative medicine. This study aimed to investigate the proliferation and adhesion of human gingival fibroblast (HGF) cells to a collagen membrane following PBM. <b>Methods:</b> Cultured HGF cells on a collagen membrane received PBM at wavelengths of 808 nm, 915 nm (2 and 4 J/cm²), and 660 nm (2.1 and 4.2 J/cm²) in interventional groups, while non-irradiated cells served as the control. On days 1 and 3 post-irradiation, cell proliferation was measured by MTT assay, and adhesion to the membrane was assessed under the scanning electron microscope (SEM). <b>Results:</b> Cell proliferation significantly increased in interventional groups compared to the control, with the most significant increase at 915 nm (4 J/cm²) in both time points. On the first day, the 808 nm and 660 nm lasers demonstrated similar results, significantly lower than the 915 nm laser (2 J/cm²). On day 3, the 660 nm, 808 nm, and 915 nm (2 J/cm²) groups showed comparable results. Qualitative analysis by the SEM identified spindle-shaped cells with multiple extended projections in 915 nm groups, especially at an energy density of 4 J/cm². Groups of 660 nm and 808 nm (4 J/cm²) showed spindle-shaped cell morphology. No distinct cellular morphology indicative of enhanced adhesion was observed at 808 nm (2 J/cm²). <b>Conclusion:</b> The most effective PBM setup for promoting HGF proliferation and adhesion to a collagen membrane was identified at 915 nm (4 J/cm²).</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e53"},"PeriodicalIF":1.4,"publicationDate":"2024-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Evaluation of Photobiomodulation and Calcium Aluminosilicate on Direct Pulp Capping of Dogs' Permanent Teeth.","authors":"Latifa Mohamed Abdelgawad, Mariam Hassan Nghnughi, Dalia El Rouby, Marwa Abdelgwad","doi":"10.34172/jlms.2024.51","DOIUrl":"10.34172/jlms.2024.51","url":null,"abstract":"<p><p><b>Introduction:</b> Photobiomodulation (PBM) is beneficial to biological tissues; depending on the optical dose that is absorbed by tissues, it can function as a biostimulative, analgesic, and anti-inflammatory mediator. Thus, the current research aimed to assess the impacts of PBM and Calcium aluminosilicate-based material on direct pulp capping (DPC) of dogs' permanent teeth through histological analysis. <b>Methods:</b> To study DPC of dogs' teeth, we separated 24 canines and premolars obtained from mature, healthy mongrel dogs into four equal groups: group 1, which served as the control (exposed pulp was covered with a sterile polytetrafluoroethylene, Teflon tape); group 2, which received PBM treatment using a 980 nm diode laser with a 100 mw output power for one minute; group 3: Calcium aluminosilicate-based material; group 4: Calcium aluminosilicate+PBM. In accordance with the assessment period, each group was divided into three equal subcategories: (A) 1 week; (B) 2 months; (C) 3 months. The teeth were evaluated histologically for inflammatory response and dentine bridge formation. <b>Results:</b> Statistical analysis detected that there was a significant difference between PBM, Calcium aluminosilicate cement, and the combination group of PBM and Calcium aluminosilicate related to the control group in variant evaluation periods regarding the inflammatory response and dentine bridge thickness through the histological analysis. In relation to the inflammatory response after one week, the combined group (Calcium silicate cement+PBM) exhibited a significantly decreased intensity of inflammation compared to other groups at an identical time. As for dentin bridge creation, the PBM+calcium aluminosilicate group detected thicker dentine bridge creation at three months than other studied groups. <b>Conclusion:</b> Combined with calcium aluminosilicate-based material, PBM using a 980 nm diode laser with output power of 100 mw for one minute decreased the initial inflammatory response and enhanced a complete thick dentine bridge formation.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e51"},"PeriodicalIF":1.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Intensity Laser Therapy - An Option for Managing the Pain in Anal Fissures.","authors":"Sônia Cristina Cordeiro Time, Ana Carolina Buffara Blitzkow, Fabrício Doin Paz de Oliveira, Luana Perondi, Elisa Treptow Marques, Abel Botelho Quaresma, Marcelo Marciano, Rodnei Bertazzo Sampietro","doi":"10.34172/jlms.2024.52","DOIUrl":"10.34172/jlms.2024.52","url":null,"abstract":"<p><p><b>Introduction:</b> Anal fissures (AFs) are a prevalent pathology. Although internal lateral sphincterotomy is still the gold-standard surgery for treating chronic AFs, this procedure is associated with a considerable risk of anal incontinence. This study describes an alternative and minimally invasive technique for treating AFs using photobiomodulation and a high-power diode laser-fissure treatment (LFT) and highlights initial results pertaining to pain. <b>Methods:</b> This retrospective study focused on 38 patients treated with LFT on an outpatient basis at three different hospitals in different states of Brazil (Santa Catarina, Paraná, and São Paulo). The objective was to evaluate the effects of LFT treatment on AF patient pain following the procedure. The Friedman test was used to identify the effects of LFT treatment over time (D0, D7, D15, D30, and D60) on postoperative pain intensity using the visual analogue scale (VAS). Complications and incontinence rates were also analyzed. <b>Results:</b> Roughly two-thirds of the patients (66%; n=25) were male. The median age of the cohort was 49. Constipation was described by 32% of the patients, 13% were smokers, and 21% had recently used opioids. In 92% of the cases, the AF was localized posteriorly. Skin tags were present in 27% of the cases, and 26% had recently undergone a previous orificial surgery. A significant reduction in pain was observed over time in the intensity of postoperative pain measured by the VAS. Prior to surgery (D0), the patients' mean VAS score was 4.1; it progressively decreased to 0.1 on the 60^th postoperative day (<i>P</i><0.05). There was no significant change in fecal continence at the end of the 60-day follow-up period. Minor complications occurred in 7.9% of the patients (2.6% hemorrhoidal thrombosis, 2.6% skin tag, and 2.6% \"failure\"). <b>Conclusion:</b> Our results suggest that treating AFs with laser therapy results in a significant reduction in pain intensity over time without interfering with anal continence.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e52"},"PeriodicalIF":1.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11625396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Photobiomodulation Using Low-Power Diode Laser Therapy and Nano-bone on Mandibular Bone Regeneration in Rats.","authors":"Latifa Mohamed Abdelgawad, Kawashty Ali Mohamed, Ahmed Abbas Zaky","doi":"10.34172/jlms.2024.50","DOIUrl":"https://doi.org/10.34172/jlms.2024.50","url":null,"abstract":"<p><p><b>Introduction:</b> Recently, the positive effects of photobiomodulation (PBM) and nano-bone on bone regeneration have garnered significant attention. The purpose of the research was to assess the impact of PBM and nano-bone on the process of mandibular bone repair in mice. <b>Methods:</b> A 4-mm diameter bone defect was created in the left mandibular angle of 24 mice separated into 4 equal groups: group I: control; group II: PBM by irradiation at 100 mW of a 980 nm diode laser for one minute (three sessions per week; day on and day off); group III: nano-bone; group IV: PBM with nano-bone. Every group was sectioned into 3 equal subgroups corresponding to the evaluation method period: (A) one week, (B) two weeks, and (C) four weeks. Histological examination was done with hematoxylin, eosin, and Masson's Trichrome after one, two and four weeks for inflammation, bone defect coverage, vascularization within the newly formed bone, and new bone formation. Statistical analysis of the data was done and presented as percentage values using chi-square. The significance level was set at <i>P</i> value≤0.05 within all tests. <b>Results:</b> In general, by histological examination of the mandibular bone defect of the rats, the intensity of inflammation was the least in group IV when compared with groups II and III and the control group at all evaluation periods (<i>P</i><0.001). Also, group IV showed a high significant rise in the percentage of new bone formation following four weeks when compared with the control (<i>P</i>≤ 0.001) and groups II and III (<i>P</i><0.001). <b>Conclusion:</b> The present research results confirmed that the combination of PBM and nano-bone can aid in the repair of mandibular bone abnormalities. This animal study suggests that the use of PBM and nano-bone should be investigated further in clinical studies.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e50"},"PeriodicalIF":1.4,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Effects of Gluma Gel, Sensodyne Repair and Protect Toothpaste, and an 810 nm Low Power Diode Laser on the Closure of Dentinal Tubules: An <i>In Vitro</i> Study.","authors":"Seyedeh Saba Sharifzadeh, Parto Nasrollahi, Arash Azizi, Shirin Lawaf","doi":"10.34172/jlms.2024.48","DOIUrl":"https://doi.org/10.34172/jlms.2024.48","url":null,"abstract":"<p><p><b>Introduction:</b> One of the common problems among dental patients is tooth hypersensitivity. There are various treatments for hypersensitivity, such as gels, solutions, toothpaste, and laser treatments. This report examined the effect of three treatments, namely 810 nm wavelength diode laser, Gluma gel, and Sensodyne Repair and Protect toothpaste, on tooth hypersensitivity. <b>Methods:</b> This experimental survey selected 40 premolars free of caries, fractures, restorations, root canal therapy, or wear. The enamel of the midline in the cervical part of the buccal surface was removed by a bur (the area was 2*2 mm and the depth was 2 mm), and the samples were then prepared and divided into four groups. Sensodyne Repair and Protect toothpaste, Gluma gel, and an 810 nm diode laser with 0.5 W power were applied to three groups of teeth. The samples were analyzed by using a field emission scanning electron microscope (FE-SEM). The data from the occluded dentinal tubules, partially occluded tubules, and open tubules were analyzed by using the Tamhane test. <b>Results:</b> The amount of occluded dentinal tubules was 83.2±9% in the laser group, 74.4±11.62% in the Gluma group, 67.1±18% in the Sensodyne group, and 15.03±3.39% in the control group. The difference between the treatment groups and the control group was meaningful (<i>P</i><0.05). However, the difference between the Gluma group, the Sensodyne group, and the 810 nm laser group was not significant (<i>P</i>>0.05). <b>Conclusion:</b> The three treatments (810 nm diode laser, Gluma, and Sensodyne) were all effective in occluding dentinal tubules compared to the control group. However, there was no significant difference between these three treatment methods (<i>P</i>>0.05).</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e48"},"PeriodicalIF":1.4,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation Improves Histological Parameters of Testis and Spermatogenesis in Adult Mice Exposed to Scrotal Hyperthermia in the Prepubertal Phase.","authors":"Fakhroddin Aghajanpour, Hojjat-Allah Abbaszadeh, Hamid Nazarian, Azar Afshar, Reza Soltani, Homayoon Bana Derakhshan, Fatemeh Fadaei Fathabadi, Mohsen Norouzian","doi":"10.34172/jlms.2024.49","DOIUrl":"https://doi.org/10.34172/jlms.2024.49","url":null,"abstract":"<p><p><b>Introduction:</b> Heat stress is one of the environmental causes of damage to the testis, whose effects are less known before puberty. The aim of the present study was to investigate the impact of photobiomodulation (PBM) on the testis of prepubertal mice subjected to hyperthermia. <b>Methods:</b> Twenty-four three-week-old prepubertal male mice were allocated to the following groups: I) control, II) scrotal hyperthermia (Hyp), and III) Hyp+PBM (n=8/each group). In order to induce hyperthermia, the scrotum was placed in water at 43 °C for 20 minutes every other day for a total duration of 10 days. In the Hyp+PBM group, after hyperthermia induction, the testis of the mice was subjected to laser irradiation at a wavelength of 890 nm (0.03 J/cm² for 30 seconds) for 35 days. After the mice were sacrificed, the testis and epididymis were removed for testing. <b>Results:</b> Compared with those of the Hyp group, the sperm parameters of the laser irradiation group improved notably. In addition, histological examinations revealed that the final number of testis cells and the volume of tissue in the Hyp+PBM group were dramatically greater than those in the Hyp group. The analysis of molecular data revealed an increase in the expression of mitotic genes and testosterone levels and a decrease in the formation of reactive oxygen species (ROS) and the expression of the apoptotic gene in the testis subjected to PBM. <b>Conclusion:</b> Based on the present findings, laser therapy can reduce complications caused by scrotal hyperthermia during prepuberty and ameliorate spermatogenesis during puberty.</p>","PeriodicalId":16224,"journal":{"name":"Journal of lasers in medical sciences","volume":"15 ","pages":"e49"},"PeriodicalIF":1.4,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142502105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}