低水平激光治疗大鼠脊髓损伤的疗效:炎症标志物和睾丸功能分析。

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Journal of lasers in medical sciences Pub Date : 2025-02-22 eCollection Date: 2025-01-01 DOI:10.34172/jlms.2025.06
Hadise Taheri, Hamid Reza Mosleh, Shima Jahanbaz, Abbas Aliaghaei, Foozhan Tahmasebinia, Marzieh Abedi, Sama Abbasi, Hojjat-Allah Abbaszadeh
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引用次数: 0

摘要

简介:脊髓损伤(SCI)是一种严重的疾病,通常导致男性持续的神经损伤和相关的生殖健康问题。本研究的目的是探讨光生物调节疗法(PBMT)在脊髓损伤炎症期的治疗潜力,以防止氧化损伤,减少炎症,减轻对睾丸功能的潜在损害。方法:18只雄性大鼠随机分为3组:A组(仅椎板切除术)、B组(挫伤)和C组(挫伤+PBMT)。损伤后30分钟,C组接受PBMT治疗2周。采用苏木精和伊红试验(H&E)进行组织学和体视学分析。脊髓损伤后8周,采用ELISA和real-time PCR检测睾丸激素、炎症和细胞凋亡的变化。结果:脊髓损伤组精子数量(平均±SD: 14.2±86.1)、活力(34.8±72.8)和活力(26.12±43.9)显著降低,炎症标志物(IL-1 β: 4.4±71.6,TNF-α: 3.14±66)水平升高,睾丸结构损伤显著。相反,与未治疗的脊髓损伤动物相比,经PBMT治疗的动物精子参数(精子数:48±34,活力:57.2±18.5,活力:52.3±88.2)显著改善,炎症(IL-1 β: 3.09±14.2,TNF-α: 2.67±74.1)显著降低。这些发现表明,PBMT通过减少炎症和保持细胞完整性来减轻脊髓损伤对睾丸组织的负面影响,从而支持其作为一种双重治疗方法来帮助神经系统恢复和维持生殖健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Therapeutic Effects of Low-Level Laser Therapy on Rat Spinal Cord Injury: Analysis of Inflammatory Markers and Testicular Function.

Introduction: Spinal cord injury (SCI) is a severe condition often leading to lasting neurological impairment and associated reproductive health issues in males. The aim of this study was to investigate the therapeutic potential of photobiomodulation therapy (PBMT) during the inflammatory phase of SCI to prevent oxidative damage, reduce inflammation, and mitigate potential damage to testicular function. Methods: Eighteen male rats were randomly divided into three groups: Group A (laminectomy only), group B (contusion), and group C (contusion+PBMT). Thirty minutes post-injury, group C received PBMT for two weeks. Histological and stereological analysis was evaluated using the hematoxylin and eosin test (H&E). ELISA and real-time PCR were performed for eight weeks post-SCI to assess testosterone and inflammatory and apoptotic changes. Statistical analysis was performed using SPSS with one-way ANOVA and Tukey's post hoc test, and statistical significance was set at P<0.05. Results: The SCI group exhibited significant reductions in sperm count (mean±SD: 14.2±86.1), motility (34.8±72.8), and viability (26.12±43.9), with increased levels of inflammatory markers (IL-1 β: 4.4±71.6, TNF-α: 3.14±66) and damage to testicular structure. In contrast, animals treated with PBMT showed significant improvements in sperm parameters (sperm count: 48±34, motility: 57.2±18.5, viability: 52.3±88.2) and a marked reduction in inflammation (IL-1 β: 3.09±14.2, TNF-α: 2.67±74.1) compared to untreated SCI animals (P<0.001). Additionally, PBMT-treated animals demonstrated significant improvements in testosterone levels (1.57±44.8) and a reversal of testicular cell loss (P<0.001). Conclusion: These findings suggest that PBMT mitigates the negative effects of SCI on testicular tissue by reducing inflammation and preserving cellular integrity, thus supporting its use as a dual therapeutic approach to aid neurological recovery and maintain reproductive health.

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来源期刊
Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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