Journal of insect physiology最新文献

{"title":"Parthenogenesis in the neotropical katydid Conocephalus ictus","authors":"Tzitzi M. Placier-Gallardo ,&nbsp;Hugo J. Amaro-Lozada ,&nbsp;Raúl Cueva del Castillo","doi":"10.1016/j.jinsphys.2025.104812","DOIUrl":"10.1016/j.jinsphys.2025.104812","url":null,"abstract":"<div><div>Parthenogenesis is a widespread reproductive strategy in insects with significant implications in the field of evolutionary biology. Nonetheless, the incidence of parthenogenesis in katydids remains low. Our findings in Los Tuxtlas, Veracruz, Mexico indicate that small proportion of females of <em>Conocephalus ictus</em> are tychoparthenogenetical, marking the first report of a tropical parthenogenetic katydid. Contrary to expectation, parthenogenesis does not appear adaptive for <em>C. ictus</em> females. Wingless parthenogenetic females outnumber their winged counterparts and exhibit larger size. In addition, parthenogenetic females have lower clutch size, hatching success, and offspring reaching adulthood compared to sexual females. The geographical distribution of parthenogenesis has been linked to ice-covered areas during glaciation periods, and the orography of Mexico suggests a potential link between the evolution of parthenogenesis in <em>C. ictus</em> and the ability to disperse effectively and colonize new environments of the parthenogenetic winged females during interglacial periods. Given its distribution, <em>C. ictus</em> offers a valuable model for understanding the impact of climatic factors on the evolutionary trajectory of parthenogenetic organisms in the tropics. Further research on <em>C. ictus</em> and its response to past climatic shifts can provide insights into the adaptive significance of parthenogenesis in evolutionary biology.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104812"},"PeriodicalIF":2.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Material composition of the endophytic ovipositor in the damselfly Calopteryx splendens (Odonata, Calopterygidae)","authors":"Natalia Matushkina ,&nbsp;Stanislav N. Gorb ,&nbsp;Wencke Krings","doi":"10.1016/j.jinsphys.2025.104813","DOIUrl":"10.1016/j.jinsphys.2025.104813","url":null,"abstract":"<div><div>Natural selection has favoured the incorporation of ions, including transition metals, in materials of various biological structures susceptible to mechanical fracture to enhance their failure and wear resistance. With regards to insects, only a few taxa have been investigated. The objective of this study was to analyse the biomechanical properties of the ovipositor in the damselfly <em>Calopteryx splendens</em> (Harris, 1780) (Odonata, Zygoptera, Calopterygidae) through nanoindentation and to ascertain the elemental composition gradient within the cuticle using energy-dispersive X-ray spectroscopy. This research represents the first report indicating that the damselfly ovipositor exhibits a gradient in the mechanical properties of the cuticle, with Young’s modulus ranging from approximately 3.0 to 7.0 GPa and hardness from 0.1 to 0.3 GPa. These properties highly correlate with the contents of copper and magnesium, both of which increase in the distal direction. The results also suggests that the mechanical properties of the cuticle are significantly influenced by the degree of sclerotization revealed by confocal laser scanning microscopy. These findings propose that the material properties of the ovipositor cuticle in <em>C. splendens</em> may have adapted to enhance piercing capability and to reduce the risk of structural failure during insertion of eggs in plant substrates.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104813"},"PeriodicalIF":2.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simulated climate warming scenarios lead to earlier emergence and increased weight loss but have no effect on overwintering survival in solitary bees","authors":"Jannik S. Möllmann,&nbsp;Liv Lörchner,&nbsp;Dean Hodapp,&nbsp;Ina Knuf,&nbsp;Hongfei Xu,&nbsp;Thomas J. Colgan","doi":"10.1016/j.jinsphys.2025.104811","DOIUrl":"10.1016/j.jinsphys.2025.104811","url":null,"abstract":"<div><div>Insect pollination is critical for both wildflower stability and agricultural yields, with solitary bees being a group of pollinators of fundamental importance. However, documented declines in populations, exacerbated by environmental pressures, including climate change, pose significant threats to the provision of ecosystem services. Exposure to elevated temperatures during periods of dormancy, such as overwintering, is predicted to lead to phenological shifts, changes in condition, and impacts on survival. However, we currently lack studies that inform how such aspects are affected in future climate change scenarios. Using simulated temperature regimes informed by predictions of the Intergovernmental Panel on Climate Change, we exposed overwintering mason bees (<em>Osmia</em> species) to three field-relevant temperature profiles based on either present-day overwintering temperatures or future temperatures predicted under two major climate warming scenarios (SSP2-4.5 and SSP5-8.5) and measured how temperature exposure affected emergence timing, weight loss, and survival. We found that exposure to temperatures under intermediate and worst-case climate warming scenarios led to earlier emergence by approximately three and six weeks, respectively, with increasing divergences in timing of emergence between the sexes of <em>Osmia bicornis</em>, which may lead to intraspecific phenological mismatches. While we found no effect of temperature on overwintering survival rates, we observed increased weight loss prior to emergence but found that in contrast to other studies, it only mildly mediated shifts in emergence timing brought about by temperature exposure, suggesting that weight loss is unlikely to play a major role as a trigger of emergence timing in mason bees. Our study contributes to the growing literature highlighting the impact that temperatures under climate change models will have on the timing of key life events for essential pollinators, which may have consequences at the population and community levels.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104811"},"PeriodicalIF":2.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNAi-mediated held-out wing (HOW) gene knockdown inhibits wing expansion of white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)","authors":"Gui-yun Long ,&nbsp;Zhao Wang ,&nbsp;Dao-chao Jin ,&nbsp;Hong Yang ,&nbsp;Sheng-jiang Yang ,&nbsp;Cao Zhou ,&nbsp;Qing-hui Zeng","doi":"10.1016/j.jinsphys.2025.104810","DOIUrl":"10.1016/j.jinsphys.2025.104810","url":null,"abstract":"<div><div>The white-backed planthopper (<em>Sogatella furcifera</em>), a major migratory pest of rice, has resistance to various chemical treatments. The developmental regulation gene “<em>held-out wing</em> (<em>HOW</em>)” gene can serve as a potential target for RNA interference (RNAi) pesticides. Here, we analyzed spatiotemporal expression pattern and biological function of <em>SfHOW</em> by RNAi-mediated gene silencing. The <em>SfHOW</em> gene contains 628 bp and encodes 196 amino acids. <em>SfHOW</em> was highly expressed in 3^rd instar nymphs, followed by 5^th instar nymphs. In different tissues, <em>SfHOW</em> is most abundantly expressed in epidermis, with lower levels in wings and legs, and the least expression observed in fat body and gut. RNAi-mediated silencing of <em>SfHOW</em> resulted in a marked decrease in its transcription levels, leading to mortality of 66.8 %. Additionally, 62.0 % emerged adults exhibited folded and curled wings, indicating that <em>SfHOW</em> is crucial for wing expansion of <em>S. furcifera</em>. Following <em>SfHOW</em> silencing, significant reductions in the expression of <em>S. furcifera Apterous</em> (<em>SfAp</em>), suggesting that <em>SfHOW</em> may regulate wing expansion by modulating <em>SfAp,</em> so as to regulate the expression of <em>Dpp</em> gene to participate in the regulation of expansion. This study identified a new target for the development of RNAi-based pesticides for rice pest control and enhances molecular understanding of wing development in Hemipteran insects.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104810"},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue- and temperature-dependent expression, enzyme activity, and RNAi knockdown of Catalase in a freeze-tolerant insect","authors":"Sarah E. Rokosh,&nbsp;Victoria E. Adams,&nbsp;Robyn Walter ,&nbsp;Grace E. Kaiser,&nbsp;Amber L. Gough,&nbsp;Jantina Toxopeus","doi":"10.1016/j.jinsphys.2025.104809","DOIUrl":"10.1016/j.jinsphys.2025.104809","url":null,"abstract":"<div><div>Organisms that overwinter in temperate climates may experience freezing and freezing-induced oxidative stress during winter. While many insect species can survive freezing, reverse genetics techniques such as RNA interference (RNAi) have not been used to understand the physiological mechanisms underlying freeze tolerance. The spring field cricket <em>Gryllus veletis</em> can survive freezing following a 6-week fall-like acclimation. We used RNAi to knock down expression of an antioxidant enzyme in <em>G. veletis</em> to test the hypothesis that minimizing oxidative stress is important for freeze tolerance. In fat body tissue, <em>Catalase</em> mRNA abundance and enzyme activity increased during the fall-like acclimation that induces freeze tolerance. Other tissues such as midgut and Malpighian tubules had more stable or lower <em>Catalase</em> expression and activity during this acclimation. In summer-acclimated (freeze-intolerant) crickets, RNA interference (RNAi) effectively knocked down production of the <em>Catalase</em> mRNA and protein in fat body and midgut, but not Malpighian tubules. In fall-acclimated (freeze-tolerant) crickets, RNAi efficacy was temperature-dependent, functioning well at warm (c. 22 °C) but not cool (15 °C or lower) temperatures. This highlights a challenge of using RNAi in organisms acclimated to low temperatures, as they may need to be warmed up for RNAi to work, potentially affecting their stress physiology. Knockdown of <em>Catalase</em> via RNAi in fall-acclimated crickets also had no effect on the ability of the crickets to survive a mild freeze treatment, suggesting that Catalase may not be necessary for freeze tolerance. Our study is the first to demonstrate that RNAi is possible in a freeze-tolerant insect, but further research is needed to examine whether other genes and antioxidants are needed for <em>G. veletis</em> freeze tolerance.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104809"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive transcriptome and metabolome analysis of the adaptability and detoxification ability of Spodoptera frugiperda larvae to tobacco","authors":"Minghui Xie ,&nbsp;Yongzhi Zhong ,&nbsp;Lulu Lin ,&nbsp;Guangling Zhang ,&nbsp;Ning Wei ,&nbsp;Feng Zhang ,&nbsp;Haoliang Chen","doi":"10.1016/j.jinsphys.2025.104800","DOIUrl":"10.1016/j.jinsphys.2025.104800","url":null,"abstract":"<div><div><em>Spodoptera frugiperda</em> is among the most significant pests affecting food crops globally. Our findings indicate that the mortality rates of 1st and 2nd instar larvae are significantly higher than those of 3rd instar and older larvae following tobacco consumption. In this study, we employed integrated transcriptomic and metabolomic analyses to investigate the changes in gene expression and metabolic processes in 2nd and 3rd instar larvae after consuming tobacco and maize. Transcriptome analysis revealed that the majority of differentially expressed genes (DEGs) involved in xenobiotic biodegradation and metabolism were upregulated, particularly cytochrome P450s. Metabolomic analysis identified alkaloid metabolites in the bodies of larvae that had fed on tobacco. The 3rd instar larvae that consumed tobacco exhibited increased production of metabolites via cytochrome P450. Correlation analysis of the transcriptome and metabolome demonstrated that, when comparing the 3rd instar larvae fed on tobacco to those fed on maize, both DEGs and differentially accumulated metabolites (DAMs) shared pathways related to cytochrome P450, fatty acid metabolism, and glutathione metabolism. Transcriptome and metabolome analysis shows cytochrome P450 play an important role in the detoxification and adaptability of <em>S. frugiperda</em> larvae to tobacco. This study provides a preliminary explanation of the detoxification metabolism and adaptive mechanisms of <em>S. frugiperda</em> larvae in response to tobacco.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104800"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of successful or unsuccessful parasitism by the solitary endoparasitoid wasp Cotesia vestalis (Hymenoptera: Braconidae) on the testis development in several host moth species","authors":"Satoshi Hiroyoshi ,&nbsp;Takayuki Mitsunaga ,&nbsp;Gadi V.P. Reddy","doi":"10.1016/j.jinsphys.2025.104808","DOIUrl":"10.1016/j.jinsphys.2025.104808","url":null,"abstract":"<div><div>Parasitism by a parasitoid wasp alters the physiology and behavior of its host insects. This study investigated the effects of successful and unsuccessful parasitization on individual host development and testis growth in several moth species. Although the solitary koinobiont endoparasitoid wasp, <em>Cotesia vestalis</em> (Hymenoptera: Braconidae), primarily parasitizes the diamondback moth (<em>Plutella xylostella</em>, Lepidoptera: Plutellidae), its host range is relatively broad. Previous research confirmed successful parasitization of <em>P. xylostella</em>, <em>Mythimna separata</em> (Noctuidae), and <em>Helicoverpa armigera armigera</em> (Noctuidae), whereas attempts to parasitize <em>Spodoptera exigua</em> (Noctuidae), <em>Palpita nigropunctalis</em> (Piralidae), <em>Aedia leucomelas</em> (Nocuidae), and <em>Mamestra brasiccae</em> (Noctuidae) were unsuccessful. In this study, testis development in all parasitized hosts, except for <em>A</em>. <em>leucomelas</em>. was suppressed or reduced compared to the unparasitized controls. Notably, testes in parasitized hosts exhibited some degree of growth after parasitization—varying by species and occurring independently of ecdysis—except in final instar larvae of <em>P. xylostella</em>. These finding suggest that parasitism suppresses or reduce testis development regardless of whether parrasistization is successful. The potential mechanisms underlying a reduction of testis development in unsuccessful parasitization are discussed in the context of ecdysteroid signaling and the parasitic process.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104808"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A C-type lectin of Helicoverpa armigera maintains the stability of the hemolymph microbiota by regulating the expression of lysozyme","authors":"Guijie Wang ,&nbsp;Jialin Wang ,&nbsp;Xusheng Liu","doi":"10.1016/j.jinsphys.2025.104799","DOIUrl":"10.1016/j.jinsphys.2025.104799","url":null,"abstract":"<div><div>An increasing body of evidence suggests that the insect hemolymph is not a sterile environment and that various nonpathogenic microorganisms can stably or transiently inhabit the hemolymph in many insect species. However, little is currently known about how the insect immune system maintains microbial homeostasis within the hemolymph. In this study, a C-type lectin of <em>Helicoverpa armigera</em> (HaCTL6) was shown to be involved in maintaining the stability of the hemolymph microbiota. The expression of <em>H. armigera</em> antimicrobial peptide (AMP) genes was down-regulated after RNAi of HaCTL6. Moreover, the knockdown of HaCTL6 resulted in a decrease in the antibacterial activity and an increase in the total bacterial load of the hemolymph. Transcriptome analysis showed that a lysozyme (HaLysozyme-like) was significantly down-regulated after HaCTL6 RNAi. Moreover, the knockdown of HaLysozyme-like led to a decrease in the antibacterial activity and an increase in the total bacterial load of the hemolymph. Furthermore, the injection of recombinant HaLysozyme-like into the hemocoel caused a significant reduction in the total number of bacteria in the hemolymph. These results indicate that HaCTL6 may regulate the homeostasis of bacteria in the hemolymph by utilizing HaLysozyme-like as an effector.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104799"},"PeriodicalIF":2.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for a command neuron controlling calling song in the cricket Gryllus assimilis","authors":"Chu-Cheng Lin,&nbsp;Berthold Hedwig","doi":"10.1016/j.jinsphys.2025.104798","DOIUrl":"10.1016/j.jinsphys.2025.104798","url":null,"abstract":"<div><div>In invertebrates stereotypical behaviours may be controlled by command neurons. In the field cricket <em>Gryllus bimaculatus</em> a command neuron descending from the brain controls the generation of rhythmic wing movements underlying calling song. We analysed if a corresponding command neuron also exists in other cricket species. Our intracellular recording and stimulation experiments point to a putative command neuron for calling song in <em>G. assimilis</em>, although the structure of the neuron could not be revealed. When this neuron was depolarised to generate 50 AP/s the cricket raised its forewings into singing position, it started rhythmic wing movements and produced the typical species-specific calling song. Further enhancing the spike rate of the neuron increased the chirp repetition rate but not the pulse repetition rate. Blocking the spike activity of the neuron by hyperpolarizing current injection reduced the chirp repetition rate and could terminate singing activity. Our evidence indicates that the two species, <em>G. assimilis</em> and <em>G. bimaculatus</em>, have homologous neurons for controlling calling song, which may be a conserved phenotype across cricket species.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104798"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein-rich trophallactic contents transferred from reproductives are crucial for termite soldier differentiation in Zootermopsis nevadensis","authors":"Hajime Yaguchi ,&nbsp;Makoto Matsushita ,&nbsp;Kiyoto Maekawa","doi":"10.1016/j.jinsphys.2025.104797","DOIUrl":"10.1016/j.jinsphys.2025.104797","url":null,"abstract":"<div><div>Polyphenic castes exist for colony success in social animals. A primer pheromone has been suggested as a well-known regulator of caste development, but the basis of chemical communication has remained elusive over the last few decades. In termites, a long-standing hypothesis is that reproductive-secreted juvenile hormone (JH) plays a role as a primer pheromone involved in soldier differentiation. Here, we tested whether exogenous JH is necessary to induce differentiation of soldiers in the dampwood termite <em>Zootermopsis nevadensis</em>. The unique characteristic of this termite is that soldier-destined individuals can be monitored during their ontogeny under natural conditions. Furthermore, oral–anal trophallactic interactions with reproductives are required for soldier differentiation. First, knockdown of JH biosynthetic genes using RNA interference (RNAi) resulted in the reduction of soldier formation but did not affect the frequency of trophallactic interaction from reproductives to RNAi-treated individuals. Next, we compared the effect of parental replacement on soldier differentiation. Old-age reproductives had fewer stimulus effects on the differentiation of soldier-destined individuals compared to young-age reproductives. This difference in stimulus effects was derived from the protein levels within the digestive fluids of the king, but not those of the queen. Consequently, there is little or no possibility that JH itself plays a role as a primer pheromone. Alternatively, we propose that protein-rich nutrients in trophallactic fluids from reproductives are sufficient to induce high JH levels in larvae required for soldier differentiation.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104797"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}