Journal of insect physiology最新文献

{"title":"Sex differences in dietary leucine utilization revealed by ¹³C tracing in the cockroach Blaptica dubia.","authors":"Ratko Pavlović, Sofia Bouchebti","doi":"10.1016/j.jinsphys.2026.105088","DOIUrl":"https://doi.org/10.1016/j.jinsphys.2026.105088","url":null,"abstract":"<p><p>Sex differences in amino acid acquisition and allocation are fundamental determinants of insect life-history strategies, yet the fate of assimilated amino acids after ingestion remains largely unknown. Here, we used ¹³C stable isotope tracing to investigate sex-specific patterns of dietary leucine utilization in the cockroach Blaptica dubia. Adult males and females were fed artificial diets containing either ¹³C-labeled or unlabeled leucine for 14 days, after which food consumption; body, gut, and fecal mass; gut length; fecal isotope enrichment; and tissue-specific ¹³C incorporation were measured. ¹³C enrichment of CO₂ was also measured. Females consumed less food than males but had substantially greater fresh and dry body mass, longer alimentary canals, and larger gut mass. Despite consuming more food and having significantly lower body mass (almost half that of females), males did not show greater ¹³C enrichment in somatic tissues; no significant sex differences were detected in the brain, fat body, muscle, or respired CO₂. In contrast, male reproductive tissues showed significantly higher isotope enrichment than female oothecae, suggesting differences in the timing and dynamics of nutrient allocation to reproduction. Male feces showed significantly greater ¹³C enrichment than female feces. In males, fecal ¹³C enrichment did not differ between days 7 and 14, whereas females showed greater enrichment on day 14, consistent with slower food transit. Together, the larger, longer alimentary canal and lower fecal ¹³C enrichment in females, combined with their lower food intake, are consistent with greater leucine retention and utilization and may indicate more efficient nutrient assimilation in females.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"105088"},"PeriodicalIF":2.6,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149227820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIFamide Signaling regulates energy mobilization during phototactic behavior in the Asian Citrus psyllid, Diaphorina citri.","authors":"Min-Er Li, Fei-Feng Wang, Kui Fang, Sheng-Feng Tu, Wen-Feng Zhao","doi":"10.1016/j.jinsphys.2026.105075","DOIUrl":"https://doi.org/10.1016/j.jinsphys.2026.105075","url":null,"abstract":"<p><p>Phototactic behavior is an energy-intensive visually guided behavior critical for insect survival and dispersal. The central neuromodulatory mechanisms underlying this oriented response remain poorly understood, particularly with respect to peptidergic regulation. To address this knowledge gap, we conducted a study on the Asian citrus psyllid, Diaphorina citri, a vector of citrus huanglongbing, whose adults exhibit robust positive phototaxis. The SIFamide (SIFa) neuropeptide (DcSIFa) and its cognate receptor (DcSIFaR) were identified, both of which showed the highest expression levels in the head. Immunofluorescence analysis revealed two pairs of SIFa-producing neurons in the pars intercerebralis with widespread brain-wide arborizations. Expression of both genes was dynamically upregulated in phototactic individuals. RNAi-mediated knockdown of either gene substantially reduced phototactic behavior, downregulated energy metabolism genes, disrupted mitochondrial respiratory function, and altered nitrogen metabolism and circadian rhythm. Physiologically, gene silencing led to reduced heart rate, lower oxygen consumption, and decreased production of adenosine triphosphate and citric acid. These findings demonstrate that SIFamide signaling acts as a central neuromodulatory hub regulating energy mobilization during phototactic behavior in D. citri, and indicate that DcSIFa or DcSIFaR may serve as promising candidate targets for pest control strategies.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"105075"},"PeriodicalIF":2.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deuterium between 31 and 1021 ppm in dietary water has no effect on lifespan in the adult housefly (Musca domestica).","authors":"Audrey Riddle, Charlie Hinson, Peter Thomson, Catherine Hambly, Juliano Morimoto, John R Speakman, Sharon E Mitchell","doi":"10.1016/j.jinsphys.2026.105078","DOIUrl":"https://doi.org/10.1016/j.jinsphys.2026.105078","url":null,"abstract":"<p><p>Deuterium, an isotope of hydrogen, exists naturally in the environment and body water at around 89-158 ppm. Reports of the health impacts of the level of deuterium in ingested water are often contradictory. We investigated the lifespan effects in houseflies fed diets made up with a range of deuterium, depleted or enriched relative to natural abundance water (31 ppm to 1021 ppm). Control houseflies were fed diets prepared with Aberdeen (57 ^oN) tap water (147 ppm). Pupae were planted individually to vials containing appropriately prepared deuterium diets. Day of eclosion, death and body weight at death were recorded. Deuterium levels of water, water from the diet and the uptake of dietary deuterium by the houseflies were confirmed using laser spectroscopy. Deuterium isotope enrichments of body water of the houseflies were correlated with the deuterium enrichment of the water in their food. However, the dietary effect was strongly attenuated. The lowest deuterium content in housefly body water was 67 ppm (for the 31 ppm water) and the highest 808 ppm for the 1021 ppm water. Levels of deuterium, between 31 and 1021 ppm, had no significant effect on lifespan, mortality risk or death body weight of the houseflies. Body weight at time of death did not vary with the levels of deuterium in either sex. In conclusion, using the housefly as a model, we did not find any support for the previous suggested impacts of deuterium content of the ingested water (between 31 and 1021 ppm deuterium) on survival or lifespan.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"105078"},"PeriodicalIF":2.6,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-redundant functions of insulin/FOXO and EcR signaling pathways regulate accessory gland structure, function and male fertility in Drosophila.","authors":"Humaira Ahmad, Anuj Kumar Pandey, Shachi Shuchi Smita, Shraddha Srivastava, Asthika Sachan, Kristipati Ravi Ram","doi":"10.1016/j.jinsphys.2026.105076","DOIUrl":"10.1016/j.jinsphys.2026.105076","url":null,"abstract":"<p><p>Somatic reproductive tissues, such as the male accessory glands in insects, are critical for insect fertility. However, the signaling pathways that maintain accessory gland homeostasis remain poorly understood. Using tissue-specific knockdown or overexpression in Drosophila, we modulated insulin signaling pathway genes in male accessory glands and examined their effects on male fertility. The impact of FOXO overexpression on gland structure and function was studied by analyzing cellular integrity, expression of genes encoding accessory gland proteins, and autophagy markers. Additionally, we explored the interaction between insulin signaling and steroid signaling by simultaneously overexpressing FOXO and knocking down the Ecdysone receptor in male accessory glands. We find that normal levels of InR, PI3K, AKT & FOXO in the accessory glands are essential for male fertility. Males with FOXO overexpression in accessory glands were sterile with reduced, structurally and functionally defective glands, possibly due to a metabolic block characterized by disrupted upstream insulin signaling and impaired autophagy, as evidenced by non-lipidated Atg8a and increased lysosomes. Both FOXO overexpression and EcR knockdown elevated glucose levels through distinct effects on insulin signaling. Unlike FOXO overexpression, EcR knockdown reduced autophagy markers. FOXO overexpression in an EcR-deficient background partially rescued gland structure and glucose levels, although the glands remained underdeveloped. Our observations highlight interactive yet non-redundant regulatory roles for insulin/FOXO and EcR signaling in accessory gland structure and function. These findings suggest that male accessory glands in insects selectively deploy distinct metabolic/hormonal input-specific stress-response programs to maintain their structural and functional integrity, as well as male fertility.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"105076"},"PeriodicalIF":2.6,"publicationDate":"2026-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149171338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stage-specific functional divergence of yolk protein genes in Spodoptera frugiperda","authors":"Yussuf Mohamed Salum,&nbsp;Solomon Joseph,&nbsp;Qingxuan Qiao,&nbsp;Xingting Zhang,&nbsp;Uroosa Zaheer,&nbsp;Zubair Ahmed,&nbsp;Faisal Munir,&nbsp;Abdalla Ibrahim Ali,&nbsp;Weiyi He,&nbsp;Yi Guo","doi":"10.1016/j.jinsphys.2026.105020","DOIUrl":"10.1016/j.jinsphys.2026.105020","url":null,"abstract":"<div><div>Yolk protein genes (<em>YPs</em>) are central to insect reproduction, yet the extent of functional divergence among members of the same gene family remains poorly understood in many pest species. Here, we identified five <em>YP</em> genes in <em>Spodoptera frugiperda</em>. Gene structure analysis showed that four <em>YP</em> genes are intronless, whereas <em>YP3</em> possesses a two-exon structure. Conserved domain analysis revealed that all five YP proteins contain a PFM_spherulin-2a-like domain. We then examined their developmental expression profiles to assess potential stage-specific specialization. <em>YP1</em> and <em>YP2</em> were highly expressed in eggs, whereas <em>YP4</em> and <em>YP5</em> were more strongly expressed during the pupal and adult stages, with <em>YP3</em> showing no marked enrichment at either targeted stage. Based on these expression patterns, four genes were selected for RNA interference assays at two developmental stages to evaluate their functions during embryogenesis and reproductive development. Knockdown of <em>YP1</em> and <em>YP2</em> in eggs significantly reduced hatchability, whereas pupal-stage knockdown of these genes did not affect fecundity or offspring hatchability. In contrast, knockdown of <em>YP4</em> and <em>YP5</em> in eggs caused little or no effect on hatchability, whereas pupal-stage knockdown reduced fecundity and offspring hatchability and impaired ovarian development. In addition, <em>YP4</em> knockdown disrupted adult emergence, leading to incomplete eclosion and malformed adults. These results show that <em>YP</em> genes in <em>S. frugiperda</em> are not functionally redundant, but instead exhibit clear stage-specific specialization. <em>YP1</em> and <em>YP2</em> are primarily associated with embryonic development and egg viability, whereas <em>YP4</em> and <em>YP5</em> contribute more to later reproductive performance, with <em>YP4</em> additionally affecting successful adult emergence. This study provides new evidence for stage-specific functional divergence within the <em>YP</em> gene family in a major agricultural pest, which may pave the ways for developing RNAi-based strategies for green pest control in <em>S. frugiperda.</em></div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"172 ","pages":"Article 105020"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148330205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel metabolic complementation pattern: The synergistic response between Sogatella furcifera and its symbionts under chitosan oligosaccharide stress sprayed on rice","authors":"Jifeng Zhang,&nbsp;Yunlong Ren,&nbsp;Danpin Zhu,&nbsp;Zhaohan Hu,&nbsp;Jiajia Li,&nbsp;Maoye Li","doi":"10.1016/j.jinsphys.2026.105019","DOIUrl":"10.1016/j.jinsphys.2026.105019","url":null,"abstract":"<div><h3>Background</h3><div>Insects and their symbionts respond synergistically to environmental changes, and metabolites are one of the important linkers in their interaction. Little is known about how plant growth regulators (PGR) affect planthoppers and their interacting symbiotic bacteria through sprayed rice.</div></div><div><h3>Results</h3><div>Here, we observed abnormalities in the planthopper's growth length and developmental duration when rice seedlings treated with near-field concentrations of chitosan oligosaccharide (COS) were fed to first-instar nymphs of the white-backed planthopper (WBPH, <em>Sogatella furcifera</em>) for one week. The differentially expressed genes of WBPH were mostly enriched in downregulated metabolic pathways, suggesting the potential involvement of its symbionts. Amplicon sequencing results validated our hypothesis that the abundance of <em>Acetobacteraceae Unclassified</em> (closely related to <em>Asaia</em> sp.) was significantly increased (from an average of 1.24% to 74.68%) under COS stress, manifested in a significant upregulation of three major metabolic pathways involving carbohydrates, lipids, and amino acids. Analysis of their shared differential metabolites and the resulting three bioindicators' interaction networks revealed a close triangular relationship among them. They exhibited reciprocal changes in main metabolic functions confirming that metabolites are important intermediaries between insects and symbiotic bacteria.</div></div><div><h3>Conclusion</h3><div>Our research provides a reference for the scientific use of PGR and the exploration of insect-symbiotic interaction mechanisms.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"172 ","pages":"Article 105019"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148329937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous microbial agents interact with housefly larvae to reshape the microbiome and accelerate cellulose degradation in silkworm (Bombyx mori) excrement","authors":"Chen Chen,&nbsp;Yuanhao Zhang,&nbsp;Yueqin Jin,&nbsp;Jieling Zhou,&nbsp;Xueping Jiang,&nbsp;Sitong Chen,&nbsp;Ran Zhang,&nbsp;Hao Li,&nbsp;Zhongzheng Gui","doi":"10.1016/j.jinsphys.2026.105017","DOIUrl":"10.1016/j.jinsphys.2026.105017","url":null,"abstract":"<div><div>Silkworm (<em>Bombyx mori</em>) excrement accumulates in large quantities and causes severe environmental pollution, with highly crystalline cellulose limiting efficient resource utilization. The development of effective cellulose-degrading bacterial technologies is crucial for advancing biotechnological applications. This study investigated the effects of exogenous microbial agents and housefly larvae composting on cellulose biodegradation in silkworm excrement. After six days, the cellulose content decreased by 52.6%, 58.2%, and 64.0% in the treatment groups, respectively, which was significantly greater than the 39.41% reduction in the control group without exogenous agents. The combination of exogenous microbial agents and housefly larvae reshaped the bacterial community, increasing the relative abundance of cellulose-degrading taxa. Specifically, Firmicutes and Actinobacteria were enriched, and the abundances of <em>Bacillus</em>, <em>Pseudomonas</em>, and <em>Cellulosimicrobium</em> increased. Functional predictions via PICRUSt indicated that carbohydrate metabolism was predicted to dominate bacterial activity, while Tax4Fun analysis suggested that exogenous agents were associated with increased predicted abundances of <em>endo</em>-β-1,4-glucanase and <em>exo</em>-β-1,4-glucanase genes in excrement, which may relate to accelerated cellulose degradation. This study suggests that the combination of exogenous microbial agents and housefly larvae could promote cellulose-degrading bacterial populations and the genetic potential for cellulase activity, representing a possible strategy for silkworm excrement bioconversion</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"172 ","pages":"Article 105017"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148264729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insulin receptor-2 regulates 20-hydroxyecdysone synthesis and ecdysis in the German cockroach","authors":"Xènia Maya-Figuerola,&nbsp;Ángel Rey-Alfonso,&nbsp;Jorge Escudero,&nbsp;José Luis Maestro","doi":"10.1016/j.jinsphys.2026.105018","DOIUrl":"10.1016/j.jinsphys.2026.105018","url":null,"abstract":"<div><div>The insulin/insulin-like growth factor signaling (IIS) pathway plays a central role in several vital physiological processes in animals. The identification of three insulin receptor (InR) (<em>BgInR1</em>, <em>BgInR2</em> and <em>BgInR3</em>) in the genome of the cockroach <em>Blattella germanica</em> (L.) prompted us to investigate into their respective contribution to the regulation of different events. Previous studies from our group revealed that <em>BgInR2</em> is the paralog that retains most of the activity in reproduction-related functions. In the present study, we focused in elucidating the roles of the three InR of <em>B. germanica</em> in molting and analyzed their involvement in the hormonal regulation underlying the different phases of this process. To do this, we first quantified the expression of several genes, including ecdysteroidogenic genes and those of peptide hormones involved in molting. Our findings indicate that the <em>ecdysis triggering hormone</em> (<em>ETH</em>) is an ecdysteroid-responsive gene. Furthermore, we analyzed the phenotype produced by RNAi-triggered specific depletion of BgInR1, BgInR2 and BgInR3, and identified BgInR2 as the key factor in IIS signaling related to molting. BgInR2 depletion produced a delay and dysregulation in ecdysone production, which in turn led to reduced and delayed <em>ETH</em> expression. Finally, this culminated in defective ecdysis and impaired exuviae shedding. Overall, our results demonstrate that the IIS pathway, via BgInR2, promotes ecdysone synthesis in the prothoracic gland, which subsequently induces ETH production to initiate and coordinate ecdysis.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"172 ","pages":"Article 105018"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148301929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mean warming enhances insect resilience to high temperatures: species-specific evidence from two wheat aphids","authors":"Xue-Jing Wang,&nbsp;Yu Wang,&nbsp;Shu-Lin Ning,&nbsp;Jia-Qi Li,&nbsp;Biao Cui,&nbsp;Long-Fei Yue,&nbsp;Li-Yan Wang,&nbsp;Chun-Sen Ma","doi":"10.1016/j.jinsphys.2026.105023","DOIUrl":"10.1016/j.jinsphys.2026.105023","url":null,"abstract":"<div><div>Under climate change, the gradual increase in average temperature is often accompanied by more frequent extreme heat events. However, their biological effects have largely been studied in isolation, overlooking the key interactions between them. Specifically, in short-lived ectotherms, it remains unclear whether mild thermal history across multiple generations alters their thermal performance, thereby influencing their response to subsequent high temperature. Here, we addressed this important issue using a full factorial experiment in which two global aphid species, <em>Sitobion avenae</em> and <em>Rhopalosiphum padi</em>, were exposed to baseline (20 ± 0 °C) and warming regimes (25 ± 0 °C and 25 ± 6 °C) for either short-term (14 days) or prolonged (60 days) durations. We then quantified their high-temperature performance, including acute thermal tolerance (CTmax), life history traits and demographic parameters at 30 °C. Our results indicated that pre-exposure to mean warming increased the capacity of both aphid species to withstand subsequent heat events, as evidenced by improved thermal tolerance and fitness-related traits at high temperatures. Notably, the magnitude and persistence of these responses differed between species. Under prolonged warming, <em>S. avenae</em> showed increases in acute heat tolerance and fitness, whereas <em>R. padi</em> improved fitness despite reduced tolerance, likely due to differences in effective acclimation temperatures. Overall, our study bridges the two interconnected facets of climate warming, providing a more realistic perspective on ectotherm resilience in a warming world.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"172 ","pages":"Article 105023"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary lipids shape honey bee (Apis mellifera) feeding and resilience to pesticide stress","authors":"Jaya Sravanthi Mokkapati,&nbsp;Christina M. Grozinger","doi":"10.1016/j.jinsphys.2026.105021","DOIUrl":"10.1016/j.jinsphys.2026.105021","url":null,"abstract":"<div><div>Pesticides are important for crop production but can have negative impacts on beneficial insects such as pollinators. In insects, physiological lipid stores may serve to sequester pesticides, while pesticide metabolism and detoxification often involve fatty acid pathways. Previous studies in <em>Apis mellifera</em> found that honey bees fed pollen with a specific protein-to-lipid ratio had improved survival when exposed to an insecticide. Here, we comprehensively investigate whether and how the type of dietary fat influences survival, energy reserves, and enzymatic biomarkers, following exposure to an organophosphate insecticide, chlorpyrifos. Newly emerged bees were fed protein-based diets enriched with either saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA) for 5 days and then exposed orally to chlorpyrifos (at LC₅₀). Bees fed MUFA and PUFA diets consumed significantly more food than those fed SFA-supplemented diet, indicating a clear preference for unsaturated fats. However, under chlorpyrifos exposure, only PUFA-fed bees exhibited significantly higher survival rates, and also maintained greater lipid reserves and acetylcholine esterase activity compared to SFA- and MUFA-fed groups. These results suggest that PUFA-enriched diets may mitigate pesticide toxicity by preserving neural enzyme function and energy homeostasis. No significant changes in glutathione-S-transferase activity were observed across treatments, suggesting limited dietary modulation of this detoxification pathway. Collectively, our findings demonstrate that dietary fatty acid composition influences honey bee physiological resilience to chlorpyrifos and highlight the potential for PUFA-based nutritional interventions or the design of pollinator habitats, to improve resilience to pesticides.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"172 ","pages":"Article 105021"},"PeriodicalIF":2.6,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148338289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}