Journal of insect physiology最新文献

Rnai-mediated knockdown of nuclear receptors impairs cuticle formation by disrupting chitin metabolic pathway in Holotrichia oblita 核糖核酸介导的核受体的敲低通过破坏几丁质代谢途径损害毛癣的角质层形成

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-21 DOI: 10.1016/j.jinsphys.2025.104861

Zhi-long Niu , Jian Wei , Qian Wang , Yu-bo Yang , Ying-Nan Gao , Lu Li , Jia Jia , Wei Guo , Dan Zhao

{"title":"Rnai-mediated knockdown of nuclear receptors impairs cuticle formation by disrupting chitin metabolic pathway in Holotrichia oblita","authors":"Zhi-long Niu , Jian Wei , Qian Wang , Yu-bo Yang , Ying-Nan Gao , Lu Li , Jia Jia , Wei Guo , Dan Zhao","doi":"10.1016/j.jinsphys.2025.104861","DOIUrl":"10.1016/j.jinsphys.2025.104861","url":null,"abstract":"<div><div>Nuclear receptors (NRs) are ligand-regulated transcription factors that play crucial roles in insect growth and development. However, their functions in <em>Holotrichia oblita</em> Faldermann remain uncharacterized. In this study, we identified and characterized three <em>HoNRs</em> from <em>H. oblita</em>. Spatiotemporal expression analysis revealed that all three <em>HoNRs</em> were predominantly expressed in eggs and first-instar larvae. Tissue localization showed high expression in the epidermis, fat body, and rectum. RNA interference (RNAi) experiments demonstrated that silencing these <em>HoNRs</em> reduced larval feeding, impaired molting, and resulted in a 37–50 % weight inhibition rate and a 73–93 % mortality rate. Hematoxylin-eosin staining showed that knockdown of <em>HoHR3</em>, <em>HoE75</em>, and <em>HoEcR</em> led to the formation of a thinner cuticle compared to controls, accompanied by a 46–48 % reduction in chitin content and downregulation of chitin metabolic Pathway genes. Additionally, the expression of these <em>HoNRs</em> was influenced by 20E at different time points. Therefore, these findings demonstrate that these three <em>HoNRs</em> are essential for normal molting in <em>H. oblita</em> through chitin metabolic pathway regulation.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104861"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional activation of BmToll9-2 to exogenous dsRNA in the larvae of Bombyx mori 家蚕幼虫BmToll9-2对外源dsRNA的转录激活。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-21 DOI: 10.1016/j.jinsphys.2025.104860

Zibing Feng , Zepeng Li , Qiuying He , Baolin Deng , Huixing Ma , Weijian Chen , Xin Li , Jie Zhang , Luc Swevers , Jisheng Liu

{"title":"Transcriptional activation of BmToll9-2 to exogenous dsRNA in the larvae of Bombyx mori","authors":"Zibing Feng , Zepeng Li , Qiuying He , Baolin Deng , Huixing Ma , Weijian Chen , Xin Li , Jie Zhang , Luc Swevers , Jisheng Liu","doi":"10.1016/j.jinsphys.2025.104860","DOIUrl":"10.1016/j.jinsphys.2025.104860","url":null,"abstract":"<div><div>The Toll pathway plays a crucial role in insect innate immunity, functioning through pattern recognition receptors (PRRs) that detect pathogen associated molecular patterns (PAMPs). Previous studies on <em>BmToll9-2</em> have primarily investigated its role in sensing bacterial pathogens and activating antimicrobial peptides. In this study, we aimed to investigate the transcriptional regulation of <em>BmToll9-2</em> in response to exogenous double-stranded RNAs (dsRNAs) in silkworm (<em>Bombyx mori</em>) larvae. Various delivery routes for dsRNA-triggered activation of <em>BmToll9-2</em> were explored. Quantitative real-time PCR (qRT-PCR) assays revealed significant up-regulation of <em>BmToll9-2</em> in the midgut, fat body, and epidermis following dsRNA injection. In contrast, oral administration of dsRNA failed to induce a transcriptional response. <em>In vitro</em> stability assays demonstrated that dsRNA was rapidly degraded in midgut fluid (within 10 min) but remains stable in the hemolymph for an extended period (up to 6 h). <em>In vivo</em> detection of dsRNA further confirmed its rapid digestion through feeding and the longer retention through injection. Additionally, infection with <em>B. mori</em> cytoplasmic polyhedrosis virus (BmCPV) also induced <em>BmToll9-2</em> expression, suggesting a link between dsRNA viruses and Toll pathway activation. Our findings suggest dsRNAs as potential PAMPs for Toll receptor signaling, highlighting the longer persistence of dsRNA in the hemolymph as a significant factor in the transcriptional response and emphasizing the role of <em>BmToll9-2</em>.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104860"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visually-guided defensive behaviors in triatomines (Heteroptera: Reduviidae) 三眼蝽的视觉引导防御行为（异翅目：夜蛾科）。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-21 DOI: 10.1016/j.jinsphys.2025.104862

Tomás Manuel Chialina , Benjamín Leonel Vidal , Sebastián A. Minoli , Martín Berón de Astrada

{"title":"Visually-guided defensive behaviors in triatomines (Heteroptera: Reduviidae)","authors":"Tomás Manuel Chialina , Benjamín Leonel Vidal , Sebastián A. Minoli , Martín Berón de Astrada","doi":"10.1016/j.jinsphys.2025.104862","DOIUrl":"10.1016/j.jinsphys.2025.104862","url":null,"abstract":"<div><div>Reduviidae is a family of insects mainly composed of predatory species known as assassin bugs and obligate blood-feeding species known as kissing bugs. Although both groups possess relatively well-developed compound eyes, image vision has only been associated with feeding behaviors in assassin bugs. Here, we studied whether vision plays a role in supporting defensive behaviors in kissing bugs.</div><div>Our findings reveal that <em>Rhodnius prolixus</em> consistently responds to visual looming stimuli by either freezing or escaping and that it can rapidly switch between these behaviors based on the ongoing stimulus information. By exposing animals to stimuli with different dynamics, we observed that escape responses were primarily evoked by stimuli mimicking the approach of a predator at a sustained velocity. Altering the contrast of the stimuli showed that freezing occurred regardless of the contrast, whereas escape was elicited only by high-contrast stimuli. Additionally, presenting numerous stimulation trials with short intertrial intervals yielded no habituation of the responses.</div><div>Our results demonstrate a clear role of the visual system in supporting defensive behaviors in kissing bugs. These responses can be evoked in a fast and flexible manner according to the ongoing information provided by the stimulus. Similar to what has been observed in highly visual arthropods, we found that the probability of occurrence and intensity of the evoked defensive responses depend on the evaluation of the impending risk that the visual stimuli entail.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104862"},"PeriodicalIF":2.3,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploration of winter diapause stages of the emerald ash borer based on morphological and biochemical parameters 基于形态学和生化参数的绿灰螟虫冬季滞育阶段的探索。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-20 DOI: 10.1016/j.jinsphys.2025.104859

Yingqiao Dang, Ke Wei, Xinyi Dai, Xiaoyi Wang

{"title":"Exploration of winter diapause stages of the emerald ash borer based on morphological and biochemical parameters","authors":"Yingqiao Dang, Ke Wei, Xinyi Dai, Xiaoyi Wang","doi":"10.1016/j.jinsphys.2025.104859","DOIUrl":"10.1016/j.jinsphys.2025.104859","url":null,"abstract":"<div><div>Diapause enables the critical survival of certain insects under adverse conditions, and the concealment ability of insects makes characterizing diapause traits challenging. Here, we focused on the invasive emerald ash borer (EAB, <em>Agrilus planipennis</em>), a species with obligatory winter diapause, and combined morphological, physiological, and biochemical analyses to correlate overwintering diapause stages with two pupal chamber forms: J-shaped (JL) and I-shaped (IL) larvae. Fourth-instar larvae (L4, nondiapausing, still feeding) outside the chamber and pupae (postdiapausing) inside the chamber served as controls. The results revealed that 1) the JL and IL periods overlapped, lasting up to 7 and 5 months, respectively. Significant variations in physiological–biochemical parameters during JL prompted its subdivision into three substages: early (JL-E), middle (JL-M), and late (JL-L). 2) Compared with L4-stage insects, insects in the JL-E and/or JL-M stages presented significantly lower juvenile hormone (JH), trehalose, glucose, and inositol amounts, whereas the 20-hydroxyecdysone (20E) and glycerol amounts increased significantly. In the JL-L and IL stages, the amounts of JH, trehalose, glucose, and inositol no longer differed from those in L4. The IL-stage insects ultimately developed into pupae, with JH, glycerol, and inositol amounts comparable to those in the pupal stage. These findings demonstrate that the JL and IL stages in <em>A. planipennis</em> exhibit strong temporal correspondence with distinct diapause phases, as evidenced by their characteristic physiological signatures. This field-based multilevel study improves the understanding of the diapause biology of this invasive insect pest.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104859"},"PeriodicalIF":2.3,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling biological age to assess maturation and ageing (Apis mellifera) 模拟生物年龄以评估成熟和衰老（蜜蜂）

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-17 DOI: 10.1016/j.jinsphys.2025.104857

Yumi Yun , Olga Frunze , Nahee Kim, Hyung-Wook Kwon

{"title":"Modeling biological age to assess maturation and ageing (Apis mellifera)","authors":"Yumi Yun , Olga Frunze , Nahee Kim, Hyung-Wook Kwon","doi":"10.1016/j.jinsphys.2025.104857","DOIUrl":"10.1016/j.jinsphys.2025.104857","url":null,"abstract":"<div><div>Environmental stresses caused by climate change—especially fluctuations in temperature and humidity—affect the behavior and physiology of honeybees (<em>Apis mellifera</em>), a model organism with a complex social structure. Worker bees, the most numerous members of colonies, perform tasks based on age polyethism, with lifespans ranging from short-lived summer bees to long-lived winter bees. While regulating this aging process and understanding how stress influences maturation are crucial for colony survival, these mechanisms remain poorly understood. To address this issue, we analyzed two physiological traits (hypopharyngeal gland development and fat body lipid mass) and the expression of six genes (<em>ilp1, ilp2, TOR1, JHAMT, AmGr10,</em> and <em>Vg</em>) in marked workers across 11 age groups (0, 3, 6, 8, 10, 12, 14, 16, 18, 21, and 25 days) during the flowering period, under controlled environmental conditions and low DWV levels. Five genes (<em>ilp1, ilp2, TOR1, JHAMT</em>, and <em>AmGr10</em>) were identified as reliable age-related markers and were used to develop a predictive model of biological age. Validation on 14-day-old workers sampled during the Flowering, Dearth, and early Overwintering Periods demonstrated that seasonal stress affects biological aging, with predicted ages of 12.14 days (flowering), 14.64 days (dearth), and 28.17 days (early overwintering). The accelerated biological aging in overwintering bees, linked with disease presence and colony failure, confirmed the model’s reliability. This study provides new insights into stress-induced aging in social insects and presents practical tools for monitoring colony health and resilience.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104857"},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Male accessory gland-derived carboxypeptidase B regulates sperm activation in the spermatophores of Grapholita molesta 雄性副腺体来源的羧肽酶B调节毛蚶精囊中精子的激活。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-17 DOI: 10.1016/j.jinsphys.2025.104858

Jie Cheng , Wei Wei , Yiyang Zhang , Fang Zhu , Zhongjian Shen , Xianwei Li , Yu Liang , Huiming Xiang , Ruiyan Ma

{"title":"Male accessory gland-derived carboxypeptidase B regulates sperm activation in the spermatophores of Grapholita molesta","authors":"Jie Cheng , Wei Wei , Yiyang Zhang , Fang Zhu , Zhongjian Shen , Xianwei Li , Yu Liang , Huiming Xiang , Ruiyan Ma","doi":"10.1016/j.jinsphys.2025.104858","DOIUrl":"10.1016/j.jinsphys.2025.104858","url":null,"abstract":"<div><div><em>Grapholita molesta</em> <!-->is a globally significant fruit-boring pest. Females maximize reproductive efficiency through optimal sperm utilization following a single mating event. Post-mating sperm activation is critical for reproductive success. Although sperm activation in <em>G. molesta</em> relies on the spermatophore microenvironment, the molecular regulators and associated metabolic pathways remain poorly understood. Here, we report that a male accessory gland-derived carboxypeptidase B (GmCPB) is essential for this process. <em>GmCPB</em> exhibited peak expression in the accessory gland during the pre-maturation stage; RNAi-mediated knockdown of <em>GmCPB</em> in males substantially reduced fertility, while mating success and longevity were unaffected. Mating with <em>GmCPB</em>-knockdown males had no effect on spermatophore formation or sperm transfer, whereas sperm activation was markedly impaired. Untargeted metabolomic analysis further revealed that multiple metabolic pathways exhibited significant dysregulation in the spermatophores of females after mating with <em>GmCPB</em>- knockdown males. Our findings propose a functional framework for CPB characterization and establish a molecular foundation for population suppression strategies targeting male accessory gland proteins in <em>G. molesta</em>.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104858"},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined transcriptome and metabolome analyses provide insights into host adaptation mechanism of Spodoptera frugiperda to cruciferous vegetables 结合转录组学和代谢组学分析，揭示了十字花科蔬菜中夜蛾对寄主的适应机制。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-17 DOI: 10.1016/j.jinsphys.2025.104856

Benshui Shu , Yuning Li , Cuiting Liu , Junying Wu , Cuiling Ye , Jintian Lin , Jingjing Zhang

{"title":"Combined transcriptome and metabolome analyses provide insights into host adaptation mechanism of Spodoptera frugiperda to cruciferous vegetables","authors":"Benshui Shu , Yuning Li , Cuiting Liu , Junying Wu , Cuiling Ye , Jintian Lin , Jingjing Zhang","doi":"10.1016/j.jinsphys.2025.104856","DOIUrl":"10.1016/j.jinsphys.2025.104856","url":null,"abstract":"<div><div>The fall armyworm (FAW), <em>Spodoptera frugiperda</em> (J. E. Smith), is a worldwide migratory pest that inflicts considerable harm on numerous economically vital crops. Cruciferous vegetables, specifically pakchoi (<em>Brassica campestris</em> L.) and purple cabbage (<em>Brassica oleracea</em> L.), are prominent cash crops in southern China; nevertheless, the suitability of larvae for these crops remains undetermined. This study examined the performance of <em>S. frugiperda</em> when fed <em>B. campestris</em> and <em>B. oleracea</em> leaves. Histopathological investigation indicated no significant alteration in the structure of the larval midgut in the <em>B. campestris</em> treatment group; however the <em>B. oleracea</em> treatment adversely affected the larval midgut structure. The activities of GST, CarE, AMS, and LPS in larval midguts from various treatment groups were studied. Comparative transcriptome analysis revealed that 793 and 3375 differentially expressed genes (DEGs) were found in the samples treated with <em>B. campestris</em> and <em>B. oleracea</em>, respectively, in comparison to the control. The DEGs that encode detoxification and digesting enzymes were subjected to additional analysis. Metabolome analysis identified 295 and 241 differential metabolites (DEMs) in the samples treated with <em>B. campestris</em> and <em>B. oleracea</em>, respectively, in comparison to the control group. KEGG pathway enrichment analysis indicated that taste transduction and purine metabolism were significantly influenced by these two cruciferous vegetables. The comprehensive analysis of these two omics datasets revealed that the metabolic pathways of amino acids, carbohydrates, and lipids were collectively enriched with DEGs and DEMs. Our findings establish a basis for subsequent investigations into the fitness mechanisms of <em>S. frugiperda</em> on cruciferous vegetables.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104856"},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of LmSPARC influences lipid metabolic homeostasis involved ovarian development in Locusta migratoria LmSPARC基因敲低影响迁徙蝗卵巢发育过程中的脂质代谢稳态。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-17 DOI: 10.1016/j.jinsphys.2025.104855

Mingjun Wang , Wenhui Guo , Yong Yang , Qingyao Zhu , Xiaohong Zhang , Daochuan Zhang

{"title":"Knockdown of LmSPARC influences lipid metabolic homeostasis involved ovarian development in Locusta migratoria","authors":"Mingjun Wang , Wenhui Guo , Yong Yang , Qingyao Zhu , Xiaohong Zhang , Daochuan Zhang","doi":"10.1016/j.jinsphys.2025.104855","DOIUrl":"10.1016/j.jinsphys.2025.104855","url":null,"abstract":"<div><div>Reproduction, a fundamental feature of all known life, is closely correlated with energy homeostasis. Lipids, which can accumulate, provide essential energy support for reproduction. Secreted Protein, Acidic and Rich in Cysteine (SPARC) is a cellular matrix glycoprotein involved in the regulation of extracellular matrix synthesis. However, the precise coordination of lipid homeostasis by SPARC during insect reproductive development remains poorly understood. Our study revealed that when the expression of <em>LmSPARC</em> was downregulated by RNA interference (RNAi) in 4th and 5th instar nymphs, molting was delayed compared with that in the control. Knockdown of <em>LmSPARC</em> reduced levels of vitellogenin (Vg) protein and retarded maturation of oocytes. The intercellular patency in the follicular epithelium of the ovaries was abnormal after <em>LmSPARC</em> silencing. In addition, transcriptomic and metabolomic analyses showed that the energy metabolism pathway was disrupted in locusts lacking <em>LmSPARC</em>, and the accumulation and degradation of lipids in the body were abnormal. This study provides insights into the effects of <em>LmSPARC</em> on ovarian development and energy metabolism homeostasis in <em>Locusta migratoria</em>, and provides a reference for screening biological control targets via on RNAi.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104855"},"PeriodicalIF":2.3,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "A physiologically-oriented transcriptomic analysis of the midgut of Tenebrio molitor". [J. Insect Physiol. 99 (2017) 58-66]. “黄粉虫中肠生理导向转录组学分析”的勘误表。[J。昆虫物理学报，99(2017)58-66。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-15 DOI: 10.1016/j.jinsphys.2025.104854

Nathalia R Moreira, Christiane Cardoso, Renata O Dias, Clelia Ferreira, Walter R Terra

引用次数: 0

Proline-rich nuclear receptor coactivator (Pnrc), the Drosophila aaquetzalli homologue, is important in survival of Henosepilachna vigintioctopunctata 富脯氨酸核受体共激活因子（Pnrc）是aaquetzalli Drosophila aaquetzalli同源物，在Henosepilachna vigintioctopunctata的生存中起重要作用。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-07-14 DOI: 10.1016/j.jinsphys.2025.104843

Ahmad Ali Anjum, Lan-Lan Liao, Lin Jin, Guo-Qing Li

{"title":"Proline-rich nuclear receptor coactivator (Pnrc), the Drosophila aaquetzalli homologue, is important in survival of Henosepilachna vigintioctopunctata","authors":"Ahmad Ali Anjum, Lan-Lan Liao, Lin Jin, Guo-Qing Li","doi":"10.1016/j.jinsphys.2025.104843","DOIUrl":"10.1016/j.jinsphys.2025.104843","url":null,"abstract":"<div><div>To develop a novel control strategy via a combination of double stranded RNA (dsRNA)-based pesticide and biocontrol in <em>Henosepilachna vigintioctopunctata</em>, we endeavored to identify potential dsRNAs that can cause living and non-feeding beetles to provide food for predators and parasitoids. In this context, depletion of proline-rich nuclear receptor coactivator (Pnrc), the <em>Drosophila melanogaster</em> aaquetzalli homologue, damages cuticle integrity. In the current paper, RNA interference (RNAi) targeting <em>Hvpnrc</em> by an injection of 200, 500 or 1000 ng of ds<em>pnrc</em> into the third instar larvae significantly reduced the level of corresponding transcript and repressed pupation and adult eclosion, in a dose-dependent manner. The resultant female adults were malformed, with misshapen elytra and hindwings. They seldom moved, consumed less potato foliage, and hardly laid eggs. Most <em>Hvpnrc</em> RNAi beetles remained alive for more than a week before death. Silencing of <em>Hvpnrc</em> at the fourth larval instar or pupal stages caused more severe defective phenotypes. The <em>Hvpnrc</em> depleted adults seldom moved and did not consume potato foliage. They did not oviposit and eventually died within 10 days post eclosion. Taken together, ds<em>pnrc</em> can cause delayed death and can exert an antifeeding effect; a ds<em>pnrc</em>-based pesticide may be compatible with biocontrol when applied to the potato field to control <em>H. vigintioctopunctata</em>.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104843"},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0