Journal of insect physiology最新文献

{"title":"How the diet influences lepidopteran reproduction: Morpho-functional, behavioral, and endocrine aspects","authors":"Evan Force,&nbsp;Matthieu Dacher,&nbsp;Stéphane Debernard","doi":"10.1016/j.jinsphys.2025.104838","DOIUrl":"10.1016/j.jinsphys.2025.104838","url":null,"abstract":"<div><div>In insects, diet influences the accomplishment of physiological functions, such as reproduction. The study of interactions between diet and reproduction, which involve morpho-functional, behavioral, and endocrine aspects, is necessary for identifying the features of the adaptive strategies of insects in their natural environment. This review delves into the intricate relationship between nutrition and reproductive processes in female and male Lepidoptera. Indeed, nutrient-rich diets are crucial for reproductive success by accelerating ovarian and testicular development, egg maturation, sperm synthesis and migration, and accessory sex gland maturation. Moreover, such diets boost the biosynthesis and release of sex pheromones and promote pheromonal detection by the olfactory system, thus eliciting sexual displays. These physiological and behavioral changes increase fertilization rate and offspring production, highlighting the importance of balanced diets in sustaining insect populations. Then, the review explores the molecular mechanisms by which the evolutionary conserved insulin signaling pathway acts as a transducer of nutritional inputs in linking with the regulatory actions of a major reproductive hormone, namely juvenile hormone. Finally, this review provides a greater comprehensive and integrated understanding of adult diet influence on lepidopteran reproduction, and also opens up new research avenues in the fields of agricultural pest control and ecotoxicology.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"164 ","pages":"Article 104838"},"PeriodicalIF":2.3,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is antibiotic (mis)use an emerging ecological threat to wild insects?","authors":"C. Ruth Archer ,&nbsp;Jana Dobelmann ,&nbsp;Vincent Doublet ,&nbsp;Svea Lindeboom ,&nbsp;Luisa Linke ,&nbsp;Lena Wilfert","doi":"10.1016/j.jinsphys.2025.104837","DOIUrl":"10.1016/j.jinsphys.2025.104837","url":null,"abstract":"<div><div>Antibiotic discovery revolutionized human healthcare and boosted agricultural productivity. The efficacy of this revolution is now being challenged however, as the release of antibiotic residues into soils and waterways promotes the evolution and spread of antibiotic resistance genes. There are signs that this antibiotic pollution also has ecological costs, including reduced insect health. Here, we assess this risk. We discuss where, when and how wild insects are exposed to antibiotics, the consequences of this exposure and crucially, if the concentrations that wild insects encounter are sufficiently high to trigger phenotypic responses. Data demonstrate that antibiotic residues reach concentrations in the field that can have phenotypic impacts in insects. These impacts include reduced health and foraging activity in pollinators, and improved survival in insect vectors of disease, demonstrating potential consequences for food-security and disease transmission. More generally, antibiotics can reduce insect stress resistance meaning that antibiotic pollution could elevate insect susceptibility to other stressors implicated in insect declines. We highlight gaps in our understanding of how antibiotic pollution affects wild insects and the ecosystem services they provide. These gaps urgently need to be filled, because global antibiotic use is rising. Crucially, we must determine how antibiotic residues in the field degrade or accumulate along food chains, and how field-realistic concentrations affect insects directly and via interactions with other environmental stressors. This will offer concrete insights into the consequences of rising antibiotic use for insects, and their broader societal and ecological impacts.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"164 ","pages":"Article 104837"},"PeriodicalIF":2.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of sterol nutrition in the fall armyworm, Spodoptera frugiperda.","authors":"Linqin Meng, Dandan Wei, Yuanze Feng, Jiancheng Zang, Weining Cheng, Xiangfeng Jing","doi":"10.1016/j.jinsphys.2025.104826","DOIUrl":"https://doi.org/10.1016/j.jinsphys.2025.104826","url":null,"abstract":"<p><p>Plant sterols constitute the sole source of sterol nutrition for insect herbivores, yet the sterol content of plants is complex and variable, and insect sterol nutrient regulation remains to be elucidated. In this study, we investigated cholesterol regulation inSpodoptera frugiperda using a geometric framework for nutrition. In the choice experiment, we provided larvae with eight food pairings. The insects did not adjust their feeding on the pair of complementary diets to achieve a specific cholesterol intake target. In the no-choice experiment, seven foods with different cholesterol concentrations were administered to explore sterol regulation. The larvae did not show a tendency to reduce or increase their intake of non-cholesterol nutrients to reach a certain cholesterol intake. We then measured the relative expression of the NPC1b gene in the intestine and the cholesterol content of the feces. As the cholesterol content in the food increased, NPC1b gene expression gradually decreased and the larvae excreted more cholesterol in the feces. Meanwhile, the cholesterol content in the larvae remains largely consistent, indicating that the larvae has a strong control on the body sterol content. Altogether, our results suggest that the larvae regulate cholesterol homeostasis by balancing cholesterol uptake and excretion, and cholesterol level can be kept within a physiologically appropriate range.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"104826"},"PeriodicalIF":2.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bombella intestini: A probiotic honeybee(Apis mellifera)gut bacterium","authors":"Meng Jie Jin ,&nbsp;Andrew B. Barron ,&nbsp;Sheng Yang He ,&nbsp;Xu Jiang He ,&nbsp;Qiang Huang ,&nbsp;Li Zhen Zhang ,&nbsp;Zi Long Wang ,&nbsp;Xiao Bo Wu ,&nbsp;Wei Yu Yan ,&nbsp;Zhi Jiang Zeng","doi":"10.1016/j.jinsphys.2025.104836","DOIUrl":"10.1016/j.jinsphys.2025.104836","url":null,"abstract":"<div><div>Honey bee colonies are facing increasing environmental stressors that threaten their health and lifespan. While the gut microbiota may play a role in honey bee physiology, the specific functions of certain bacterial species remain unclear. This study investigates whether <em>Bombella intestini</em>, a bacterium highly enriched in the queen gut but nearly absent in worker bees, can act as a probiotic to promote honey bee growth, metabolism, and lifespan. Our results show that <em>B. intestini</em> can survive in larval food and the larval gut. When larval food is inoculated with <em>B. intestini</em> there is increased tryptophan in both the larval diet and larval hemolymph. Bees fed this diet had a longer lifespan. This study identifies <em>B. intestini</em> as a potential probiotic for honey bees, providing a microbiome-based strategy to enhance their growth and longevity. These findings open new avenues for improving honey bee health management through microbial supplementation.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"164 ","pages":"Article 104836"},"PeriodicalIF":2.3,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual dimorphism of tarsal structures and attachment forces in Diabrotica virgifera virgifera LeConte (Coleoptera, Chrysomelidae, Galerucinae).","authors":"Dagmar Voigt, Thomas Thieme, Kai Gloyna, Christoph Buuk, Stanislav Gorb","doi":"10.1016/j.jinsphys.2025.104835","DOIUrl":"https://doi.org/10.1016/j.jinsphys.2025.104835","url":null,"abstract":"<p><p>Agile Diabrotica virgifera virgifera beetles reliably attach and walk on a wide range of substrates, including soil, waxy host plants, non-host plants, and female elytra. This ability relies on a complex of features, including insect behavior and effective tarsal structures. The detailed male and female tarsal design, dimension, geometry, and performance on the counterpart substrates were contents of the present comparative study. The presence of tarsal adhesive setae with lanceolate, filamentous, spatulate, and discoid terminals was confirmed. Male-specific adhesive setae occurred in ovoid patches on the first tarsomere of the fore- and midlegs, including two subtypes (long, spatulate with discoid-oval terminal and short, mushroom-shaped with circular terminal). The mushroom-shaped one appeared to be extraordinary, which has not been found in other leaf beetles so far. Summarizing the widths of all thin-film elements (adhesive terminals) potentially participating in contact formation resulted in the overall peeling line of all adhesive pads of 1.1 cm and 1.0 cm in males and females, respectively. The attachment force normalized by the total peeling line length (N m^-1) in males (0.9) outperformed that of females (0.2). According to linear models, statistical interactions occurred between (i) sex and glass surface geometry, (ii) glass surface wettability and geometry, (iii) sex, glass surface wettability and geometry, as well as sex and plant substrate geometry. Pull-off force depended significantly on sex and substrate. Reliable attachment in D. virgifera virgifera on host plants and during mating depends on (i) dimension and geometry of adhesive structures, (ii) substrate dimension and geometry, (iii) involved secretions (tarsal fluid, epicuticular grease), (iv) leg and feet postures (angles of segments to each other and the substrate), as well as (v) beetles' motions and behaviour. We suggest balanced attachment-detachment procedures that suit the mobile lifestyle of the species studied.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"104835"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of topical application as a delivery method of double-stranded RNA in Rhodnius prolixus (Hemiptera, Triatominae) for salivary gene silencing","authors":"Marcela Cristina Raso Mattos ,&nbsp;Maurício Roberto Viana Sant’Anna ,&nbsp;Daniela Viana Santos ,&nbsp;Lidiane Medeiros Costa ,&nbsp;Letícia Beatriz Silva Almeida ,&nbsp;Grasielle Caldas D’Avila Pessoa ,&nbsp;Leonardo Barbosa Koerich ,&nbsp;Ricardo Nascimento Araujo ,&nbsp;Nelder Figueiredo Gontijo ,&nbsp;Marcos Horácio Pereira","doi":"10.1016/j.jinsphys.2025.104820","DOIUrl":"10.1016/j.jinsphys.2025.104820","url":null,"abstract":"<div><div>RNA interference (RNAi) techniques have been widely applied in insect pest control and genomics research, effectively targeting genes in tissues like salivary glands and the midgut in Triatominae. This study evaluated the efficacy of topical application as a delivery method for dsRNA targeting <em>Rhodnius prolixus</em> salivary genes, Nitrophorin 2 (<em>NP2</em>), and Nitric Oxide Synthase (<em>NOS</em>). Applying dsRNA diluted in acetone (1:1) to the dorsal side of nymphs successfully triggered RNAi, achieving transstadial mRNA reduction while preserving dsRNA integrity. Real-time quantitative polymerase chain reaction (qPCR) analysis showed significant reductions on mRNA levels of <em>NP2</em> (72 % to 95.6 %) across N2 to N5 stages, with no off-target effects in other nitrophorins (<em>NP1–NP4</em>), confirming silencing specificity. Similarly, <em>NOS</em> knockdown reduced mRNA levels by 47.9 % to 91.0 % across N1 to N5 stages. These reductions were accompanied by visible color changes in salivary glands, a phenotype validated through image analysis. Functional assays further demonstrated that <em>NP2</em> silencing shortened plasma coagulation time, while <em>NOS</em> knockdown reduced the proportion of NO-bound nitrophorins. Compared to microinjection, the topical application of 2.5 µg of dsRNA (<em>NP2</em> or <em>NOS</em>) in N3 was less effective in reducing mRNA levels (qPCR) as well as in altering insect phenotypes (plasma recalcification time or spectrophotometry analysis). Although less efficient than microinjection, topical application as a delivery method offers advantages: it avoids physical damage to insects, simplifies application for smaller nymphs, and provides a practical alternative for studying immune-related genes. These findings highlight the potential of topical RNAi as a non-invasive, effective tool for functional genomic studies in Triatominae, particularly for salivary gland targets.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104820"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three antenna-specific odorant binding proteins in Protaetia brevitarsis (Coleoptera: Scarabaeoidea) involve in recognition of floral volatiles","authors":"Panjing Liu ,&nbsp;Xiaofang Zhang ,&nbsp;Yubo Wang ,&nbsp;Beibei Xiao ,&nbsp;Qianfu Su ,&nbsp;Tao Zhang ,&nbsp;Hongyi Wei","doi":"10.1016/j.jinsphys.2025.104823","DOIUrl":"10.1016/j.jinsphys.2025.104823","url":null,"abstract":"<div><div>Odorant binding proteins (OBPs) play a vital role in the insect olfactory recognition system, as they bind and transport specific semiochemicals to chemosensory receptors for further processing. The agricultural and horticultural pest, white-spotted flower chafer (WSFC), <em>Protaetia brevitarsis</em> (Coleoptera, Scarabaeoidea), has significantly harmed numerous crops and fruits in China. It is hypothesized that WSFCs rely on specific OBPs and chemosensory receptors to identify palatable food sources and optimal oviposition sites. Twenty-three putative OBPs (PbreOBPs) were identified from the WSFC genome in our research. Based on phylogenetic analysis, PbreOBPs exhibited high degree of similarity to those of <em>Holotrichia parallela</em> and <em>Anomala corpulenta</em>. Tissue expression profiles showed that ten PbreOBPs exhibited antenna-biased expression. We recombined and purified three antenna-specific PbreOBPs: PbreOBP8, PbreOBP9, and PbreOBP18. Fluorescence competitive binding assays revealed that they had higher binding affinities to <em>β</em>-ionone, phenethyl salicylate, phenylacetaldehyde, and benzyl benzoate — compounds commonly found in floral volatiles. Additionally, PbreOBP8 showed the ability to bind microbial volatiles, such as 3-octanol, 3-methyl-1-butanol, and 1-octen-3-ol, suggesting a role in locating food or oviposition sites. Forthermore, we also predicted the key acid residues which involved in the binding of three PbreOBPs towards floral and microbial volatiles. The present investigation demonstrated that the antenna-specific PbreOBP8, PbreOBP9 and PbreOBP18 were likely involved in mediating the recognition of floral volatiles. The findings introduce possible avenues for further research into PbreOBPs and provide potential targets for creating olfactory-based control methods for WSFC populations.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"164 ","pages":"Article 104823"},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First study on the effect of UV radiation on spiders: biochemical analysis","authors":"A. Laino ,&nbsp;C. Gabellone ,&nbsp;M. Gómez Lobato ,&nbsp;F. Arrighetti ,&nbsp;J. Tau ,&nbsp;S. Rodríguez Gil ,&nbsp;C.F. Garcia","doi":"10.1016/j.jinsphys.2025.104821","DOIUrl":"10.1016/j.jinsphys.2025.104821","url":null,"abstract":"<div><div>Ultraviolet radiation is one of the environmental factors that impacts all living beings and can cause various types of damage. In this study, the effects of UV-B and UV-C radiation on the antioxidant response, reactive oxygen species levels, and potential histological and chromosomal damage were evaluated for the first time in the spider <em>Misumenops maculissparsus</em> at three developmental stages (juveniles J3, J4, and adults). Juveniles exhibited significant inhibition of the antioxidant enzymes superoxide dismutase, catalase, and glutathione-S-transferase under UV radiation exposure. In adults, no enzymatic activity changes were observed, but there were increases in ROS levels, particularly under UV-C exposure, and alterations in the cellular population analyzed by flow cytometry. Histological analysis revealed substantial pigment accumulation beneath the cuticle in juveniles and, to a lesser extent, in adults, which could serve as a natural barrier and antioxidant defense against UV radiation. Cytogenetic analysis determined for the first time in this species a diploid chromosome number of 2n = 23 and identified chromatin granules in UV radiation-exposed adults, suggesting UV radiation-induced chromatin damage. These multidisciplinary findings enhance our understanding of the physiological defenses that spiders employ when exposed to UV radiation at different developmental stages.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104821"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing nanoparticle-enabled dsRNA delivery for oral RNAi in two orthopteran pests: Schistocerca gregaria and Melanoplus sanguinipes","authors":"Seema Rana ,&nbsp;Changsun Kang ,&nbsp;Seonghyun Ryu ,&nbsp;Derek A. Woller ,&nbsp;Dongin Kim ,&nbsp;Hojun Song","doi":"10.1016/j.jinsphys.2025.104825","DOIUrl":"10.1016/j.jinsphys.2025.104825","url":null,"abstract":"<div><div>Locusts and pest grasshoppers (Orthoptera: Acrididae) cause significant economic losses to agricultural crops and rangeland forage and can even cause humanitarian crises during periodic plagues. Current management methods for these insects rely heavily on broad-spectrum chemical insecticides and growth regulators, which can affect non-target organisms and may eventually develop resistance in the targeted species. Therefore, we assessed the potential of RNA interference (RNAi)-based alternative strategies that could supplement the current management methods. In insects, RNAi efficiency is known to vary with the method of double-stranded RNA (dsRNA) delivery. In this study, we tested two different delivery methods (injection and oral feeding) in the desert locust (<em>Schistocerca gregaria</em>) and the migratory grasshopper (<em>Melanoplus sanguinipes</em>) and showed that both species are sensitive to the injection but not to the oral feeding of dsRNA, likely due to high nuclease activity or poor uptake in the midgut. To address these limitations, we explored the utility of using nanoparticles that are often used for drug delivery in humans as a carrier (poly lactic-co-glycolic acid [PLGA] and poly(L-arginine)-polyethylene glycol [PLA-PEG]) for orally delivering dsRNA to the insect pests. Although the PLGA nanoparticles successfully permeated the digestive system into the hemolymph and the PLA-PEG-dsRNA complexes remained stable in the midgut juice and were detected in the fat body, neither dsRNA-encapsulating nanoparticle elicited gene knockdown upon oral feeding. These results suggest that nanoparticle-based oral delivery improves dsRNA stability and midgut permeation. However, additional barriers must be overcome to achieve efficient oral RNAi in these orthopteran pest species.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104825"},"PeriodicalIF":2.3,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Termite queen physogastry and associated mechanisms shaping a high lifetime fecundity","authors":"Ana Maria Costa-Leonardo ,&nbsp;Vanelize Janei ,&nbsp;Ana Cristina Ignatti ,&nbsp;Iago Bueno da Silva","doi":"10.1016/j.jinsphys.2025.104824","DOIUrl":"10.1016/j.jinsphys.2025.104824","url":null,"abstract":"<div><div>Termites are eusocial insects whose colonies are usually headed by a pair of reproductives (queen and king). During its lifespan, the royal couple faces an age-dependent increase in fecundity. In queens, it reflects on the disproportional development of the ovaries and enlargement of the abdominal cuticle, a phenomenon referred to as physogastry. Beyond the impact on the ovaries and egg production, physogastry requires the morphological and physiological reprogramming of other systems such as the digestive, tracheal, muscular, and circulatory, resulting in a process that takes years to happen. Given the sampling difficulty and the absence of physogastric queens in some model species, such a topic is overlooked, and a comprehensive review has not been provided to date. Here we aim to review the process of physogastry in termite queens, from the pioneer studies on the morphological changes accompanying this process to recent advances in the molecular mechanisms underlying fecundity and the remarkable longevity reached by mature queens (e.g., TI-J-LiFe framework). Intrinsic and extrinsic factors driving this phenomenon, its impact on colony growth, and the current knowledge gaps are also discussed. We expect that our approach paves the way for further studies of termite physogastry, especially given the advances of molecular tools and recent termite phylogenies, and comparative analyses with other eusocial insects and the mechanisms driving this phenomenon in the different groups. Physogastry is a rare process in adult insects and remarkable in termite queens, offering a<!--> <!-->unique opportunity to unravel the basis of a highly fecund life.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104824"},"PeriodicalIF":2.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}