一种胰岛素样肽介导褐藻糖储存在西部褐变植物昆虫,Lygus hesperus。

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
Devin T Mazolewski, J Joe Hull, Colin S Brent, Andrew B Nuss
{"title":"一种胰岛素样肽介导褐藻糖储存在西部褐变植物昆虫,Lygus hesperus。","authors":"Devin T Mazolewski, J Joe Hull, Colin S Brent, Andrew B Nuss","doi":"10.1016/j.jinsphys.2025.104885","DOIUrl":null,"url":null,"abstract":"<p><p>Insulin signaling controls many physiological processes in insects, and it has a demonstrated role in cellular uptake of circulating sugars. Although model insects have pioneered much of our understanding of insulin signaling, high throughput genetic sequencing has enabled opportunities for the physiological study of less explored insect species. Lygus hesperus, the western tarnished plant bug, is a significant agricultural pest of numerous crops and recent efforts have focused on molecular approaches for identifying new pest management strategies. In this work, three insulin like peptides (LhILP1, LhILP2, and LhILP3) were characterized from the transcriptome of L. hesperus. LhILP1 and LhILP2 structurally resemble classic insulin like peptides while LhILP3 resembles arthropod Insulin-like Growth Factors (aIGFs). All three LhILPs were primarily expressed in the head, and were observed throughout development. We examined their function by observing clearance rates of injected trehalose from the hemolymph in adult L. hesperus males following RNAi knockdown of the respective LhILPs. Untreated males cleared the trehalose within 6 h and showed a corresponding increase in glycogen content. While knockdown of LhILP1 did not impact clearance rate, knockdown of LhILP2 prevented clearance of circulating trehalose and glycogen accumulation. Knockdown of LhILP3 also prevented trehalose clearance, but this appears to be influenced by co-knockdown of LhILP2, rather than a direct effect. Head ligation stopped the release of head-produced LhILPs, preventing the clearance of injected trehalose, a condition that could be rescued with co-injection of heterologous insulin. Stage-specific expression in LhILP RNAi insects suggest that LhILPs have multiple additional roles besides hemolymph carbohydrate homeostasis in L. hesperus that remain to be explored.</p>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":" ","pages":"104885"},"PeriodicalIF":2.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An insulin-like peptide mediates trehalose storage in the western tarnished plant bug, Lygus hesperus.\",\"authors\":\"Devin T Mazolewski, J Joe Hull, Colin S Brent, Andrew B Nuss\",\"doi\":\"10.1016/j.jinsphys.2025.104885\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Insulin signaling controls many physiological processes in insects, and it has a demonstrated role in cellular uptake of circulating sugars. Although model insects have pioneered much of our understanding of insulin signaling, high throughput genetic sequencing has enabled opportunities for the physiological study of less explored insect species. Lygus hesperus, the western tarnished plant bug, is a significant agricultural pest of numerous crops and recent efforts have focused on molecular approaches for identifying new pest management strategies. In this work, three insulin like peptides (LhILP1, LhILP2, and LhILP3) were characterized from the transcriptome of L. hesperus. LhILP1 and LhILP2 structurally resemble classic insulin like peptides while LhILP3 resembles arthropod Insulin-like Growth Factors (aIGFs). All three LhILPs were primarily expressed in the head, and were observed throughout development. We examined their function by observing clearance rates of injected trehalose from the hemolymph in adult L. hesperus males following RNAi knockdown of the respective LhILPs. Untreated males cleared the trehalose within 6 h and showed a corresponding increase in glycogen content. While knockdown of LhILP1 did not impact clearance rate, knockdown of LhILP2 prevented clearance of circulating trehalose and glycogen accumulation. Knockdown of LhILP3 also prevented trehalose clearance, but this appears to be influenced by co-knockdown of LhILP2, rather than a direct effect. Head ligation stopped the release of head-produced LhILPs, preventing the clearance of injected trehalose, a condition that could be rescued with co-injection of heterologous insulin. Stage-specific expression in LhILP RNAi insects suggest that LhILPs have multiple additional roles besides hemolymph carbohydrate homeostasis in L. hesperus that remain to be explored.</p>\",\"PeriodicalId\":16189,\"journal\":{\"name\":\"Journal of insect physiology\",\"volume\":\" \",\"pages\":\"104885\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of insect physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jinsphys.2025.104885\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.jinsphys.2025.104885","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

胰岛素信号控制昆虫的许多生理过程,并在循环糖的细胞摄取中发挥作用。虽然模式昆虫在我们对胰岛素信号的理解方面是先驱,但高通量基因测序为较少探索的昆虫物种的生理研究提供了机会。西部褐蝽(Lygus hesperus)是一种重要的农业害虫,是许多作物的主要害虫,最近的努力集中在分子方法上,以确定新的害虫管理策略。在这项工作中,从L. hesperus转录组中鉴定了3种胰岛素样肽(LhILP1, LhILP2和LhILP3)。LhILP1和LhILP2在结构上类似于经典的胰岛素样肽,而LhILP3类似于节肢动物胰岛素样生长因子(aigf)。所有三种LhILPs主要在头部表达,并在整个发育过程中观察到。我们通过观察在RNAi敲除相应的LhILPs后,从成年雄性L. hesperus的血淋巴中注射海藻糖的清除率来检测它们的功能。未经处理的雄鼠在6 h内清除海藻糖,糖原含量相应增加。虽然LhILP1的敲低不影响清除率,但LhILP2的敲低阻止了循环海藻糖和糖原积累的清除。LhILP3的敲低也会阻止海藻糖的清除,但这似乎受到LhILP2的共同敲低的影响,而不是直接影响。头部结扎阻止了头部产生的LhILPs的释放,阻止了注射海藻糖的清除,这种情况可以通过联合注射异种胰岛素来挽救。在LhILP RNAi昆虫中的阶段特异性表达表明,LhILP除了在L. hesperus中维持血淋巴碳水化合物稳态外,还具有多种其他作用,这些作用仍有待探索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An insulin-like peptide mediates trehalose storage in the western tarnished plant bug, Lygus hesperus.

Insulin signaling controls many physiological processes in insects, and it has a demonstrated role in cellular uptake of circulating sugars. Although model insects have pioneered much of our understanding of insulin signaling, high throughput genetic sequencing has enabled opportunities for the physiological study of less explored insect species. Lygus hesperus, the western tarnished plant bug, is a significant agricultural pest of numerous crops and recent efforts have focused on molecular approaches for identifying new pest management strategies. In this work, three insulin like peptides (LhILP1, LhILP2, and LhILP3) were characterized from the transcriptome of L. hesperus. LhILP1 and LhILP2 structurally resemble classic insulin like peptides while LhILP3 resembles arthropod Insulin-like Growth Factors (aIGFs). All three LhILPs were primarily expressed in the head, and were observed throughout development. We examined their function by observing clearance rates of injected trehalose from the hemolymph in adult L. hesperus males following RNAi knockdown of the respective LhILPs. Untreated males cleared the trehalose within 6 h and showed a corresponding increase in glycogen content. While knockdown of LhILP1 did not impact clearance rate, knockdown of LhILP2 prevented clearance of circulating trehalose and glycogen accumulation. Knockdown of LhILP3 also prevented trehalose clearance, but this appears to be influenced by co-knockdown of LhILP2, rather than a direct effect. Head ligation stopped the release of head-produced LhILPs, preventing the clearance of injected trehalose, a condition that could be rescued with co-injection of heterologous insulin. Stage-specific expression in LhILP RNAi insects suggest that LhILPs have multiple additional roles besides hemolymph carbohydrate homeostasis in L. hesperus that remain to be explored.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信