Journal of insect physiology最新文献_第2页

BR-C promotes lipid synthesis through the nuclear receptor HR96 during metamorphosis in insects BR-C在家蚕和果蝇的变态过程中通过核受体HR96促进脂质合成。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-01-25 DOI: 10.1016/j.jinsphys.2026.104946

Xing Zhang , Peixin Li , Xuechuan He , Dongqin Yuan , Hao Li , Qiaoling Sun , Daojun Cheng , Wenliang Qian

{"title":"BR-C promotes lipid synthesis through the nuclear receptor HR96 during metamorphosis in insects","authors":"Xing Zhang , Peixin Li , Xuechuan He , Dongqin Yuan , Hao Li , Qiaoling Sun , Daojun Cheng , Wenliang Qian","doi":"10.1016/j.jinsphys.2026.104946","DOIUrl":"10.1016/j.jinsphys.2026.104946","url":null,"abstract":"<div><div>Insect metamorphosis needs substantial energy mobilization, and lipid serves as a crucial energy resource during the non-feeding pupal stage. The Broad Complex (BR-C) acts as a critical mediator of ecdysone signaling, which is the key regulator of insect metamorphosis. While the functions of BR-C in developmental transitions have been extensively characterized, its potential role in regulating lipid metabolism remains largely unexplored. Here, we demonstrate that BR-C is essential for lipid synthesis in the fat body during the larval-pupal transition. <em>BR-C</em> knockdown in both <em>Bombyx</em> and <em>Drosophila</em> resulted in significantly reduced lipid contents in the fat body and downregulated transcription of critical lipid synthase genes, including long-chain acyl-CoA synthetase (Acsl) and phosphatidic acid phosphatase (Lipin). We further identified the nuclear receptor gene <em>HR96</em> as a direct target of BR-C and showed that <em>HR96</em> knockdown phenocopied the reduced lipid synthesis that caused by <em>BR-C</em> knockdown. Mechanistically, we revealed that HR96 protein directly binds to the promoter regions of <em>Acsl</em> and <em>Lipin</em> to activate their transcription. Together, our findings establish a novel BR-C-HR96-lipid synthesis cascade that is functionally conserved in insects.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104946"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-Wide Identification and functional characterization of chitinase genes in the sorghum aphid (Melanaphis sacchari) 高粱蚜虫几丁质酶基因的全基因组鉴定和功能鉴定。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-01-17 DOI: 10.1016/j.jinsphys.2026.104943

Minghui Guan , Lu Sun , Chengzhi Ye , Jijuan Li , Shengyun Shan , Xiaofei Li , Junli Du , Degong Wu

{"title":"Genome-Wide Identification and functional characterization of chitinase genes in the sorghum aphid (Melanaphis sacchari)","authors":"Minghui Guan , Lu Sun , Chengzhi Ye , Jijuan Li , Shengyun Shan , Xiaofei Li , Junli Du , Degong Wu","doi":"10.1016/j.jinsphys.2026.104943","DOIUrl":"10.1016/j.jinsphys.2026.104943","url":null,"abstract":"<div><div>Insect chitinases are integral to a spectrum of vital physiological processes, including growth, development, molting, and metamorphosis. Consequently, chitin metabolic enzymes are potential targets for eco-friendly insecticide development. This study employed bioinformatics approaches to analyze the chitinase gene family in the sorghum aphid(<em>Melanaphis sacchari</em>), identifying seven <em>MsCht</em> genes. Phylogenetic distribution analysis revealed that the chitinases of the sorghum aphid are distributed across multiple clades, and the presence of several orthologous relationships with the pea aphid suggests a relatively closer evolutionary relationship between the two species. Collinearity analysis indicated minimal in the homologous evolutionary relationships among genes of closely related species. Age-stage and tissue expression analysis revealed relatively high expression levels of <em>MsCht</em> genes in 1L2d and 3L2d nymphs, along with the thoracic region, abdomen, and legs. Subsequent plant-mediated RNAi assays confirmed that silencing <em>MsCht</em> genes, effectively disrupted ecdysis, causing severe morphological defects and significantly increased mortality over time. These findings functionally validate chitinases as promising RNAi targets for sustainable management of <em>M. sacchari</em>.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104943"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cell growth progression and short-term slight temperature changes on mitochondrial physiology of Drosophila melanogaster embryonic cell line 细胞生长进程和短期轻微温度变化对黑腹果蝇胚胎细胞系线粒体生理的影响。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-02-05 DOI: 10.1016/j.jinsphys.2026.104948

Rodiésley S. Rosa , Ana Paula M. Mendonça , Matheus P. Oliveira , Danielle B. Carvalho , Leonardo Vazquez , Marcus F. Oliveira

{"title":"Effects of cell growth progression and short-term slight temperature changes on mitochondrial physiology of Drosophila melanogaster embryonic cell line","authors":"Rodiésley S. Rosa , Ana Paula M. Mendonça , Matheus P. Oliveira , Danielle B. Carvalho , Leonardo Vazquez , Marcus F. Oliveira","doi":"10.1016/j.jinsphys.2026.104948","DOIUrl":"10.1016/j.jinsphys.2026.104948","url":null,"abstract":"<div><div>The fruit fly <em>Drosophila melanogaster</em> is a valuable model for studying cellular and metabolic processes conserved with mammals. Using its embryonic Schneider S2 cell line, a widely employed system for heterologous protein expression, we investigated how cell growth progression and short-term slight temperature changes affect mitochondrial physiology via high-resolution respirometry. We assessed five mitochondrial bioenergetic parameters and two coupling parameters at 2<sup>nd</sup> (early) and 7<sup>th</sup> (late) days of culture, and at two temperatures (26°C and 28°C). We observed that culture time is the overwhelming driver of cell density variation, while temperature effect is only modest. Importantly, a modest 2°C increase, combined with progression to the late growth phase, synergistically enhanced basal, OXPHOS, and leak respiration, while only marginally affecting coupling efficiency. Notably, spare and maximal respiratory capacities rose disproportionately with either cell growth or mild temperature increase, suggesting mitochondrial biogenesis and functional remodeling. These findings reveal that minor, short-term temperature shifts can elicit rapid mitochondrial acclimation that enhances bioenergetic capacity and metabolic flexibility without compromising efficiency. Such responses exemplify mitochondrial plasticity to environmental and developmental cues and may inform strategies to modulate mitochondrial physiology across biological and biomedical contexts.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104948"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BmTrpA1-Mediated ILP/AKT/FOXO signaling Underlies Temperature-Dependent diapause in Silkworms, Bombyx mori bmtrpa1介导的ILP/AKT/FOXO信号是家蚕温度依赖性滞育的基础。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-01-17 DOI: 10.1016/j.jinsphys.2026.104939

Yizhong Zhang , Haoping She , Shanshan Wang , Qianyi Cui , Wenjie Zhang , Shunming Tang , Xingjia Shen , Juan Zhu

{"title":"BmTrpA1-Mediated ILP/AKT/FOXO signaling Underlies Temperature-Dependent diapause in Silkworms, Bombyx mori","authors":"Yizhong Zhang , Haoping She , Shanshan Wang , Qianyi Cui , Wenjie Zhang , Shunming Tang , Xingjia Shen , Juan Zhu","doi":"10.1016/j.jinsphys.2026.104939","DOIUrl":"10.1016/j.jinsphys.2026.104939","url":null,"abstract":"<div><div>Diapause is a developmentally arrested state in insects induced by seasonal cues such as photoperiod and temperature. The scientific literature agrees on that the temperature-sensitive transient receptor potential ankyrin 1 (TRPA1) channel and insulin signaling pathway (ILP/AKT/FOXO) are both essential for diapause regulation, but their interactions remain unclear. This study investigates whether the ILP/AKT/FOXO pathway can mediate the temperature-dependent maternal embryonic diapause induced by the TRPA1 channel in <em>Bombyx mori</em>. We found that the maternal pupae primed for diapause-egg-production (25DD) and those primed for non-diapause-egg-production (15DD) significantly differ in temporal patterns of <em>BmTrpA1</em> gene expression. Next, we used the <em>BmTrpA1</em><sup>-/-</sup> mutant, and showed that the mutation results in abolishing the transient peak of diapause hormone (DH) concentration in hemolymph, affects the protein profile of ILP, mRNA profiles of <em>AKT</em> and <em>FOXO</em>, and phosphorylation of FOXO protein. Generally, the profiles in <em>BmTrpA1</em><sup>-/-</sup> mutants were similar to those of 15DD pupae. In addition, we found that RNAi-mediated silencing of <em>FOXO</em> in the <em>BmTrpA1</em><sup>-/-</sup> mutant, or <em>AKT</em> in 15DD pupae, reduces or increases the diapause incidence, respectively. Collectively, our results indicate that the temperature-dependent diapause regulation by the TRPA1 channel is mediated by the ILP/AKT/FOXO pathway in the silkworm.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104939"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic heat stress disrupts foraging motivation in honey bees 慢性热应激会破坏蜜蜂的觅食动机。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-01-25 DOI: 10.1016/j.jinsphys.2026.104945

Olga Frunze , Hyunjee Kim , Soon Ok Woo , Seung-Jae Lee , Hyung-Wook Kwon

{"title":"Chronic heat stress disrupts foraging motivation in honey bees","authors":"Olga Frunze , Hyunjee Kim , Soon Ok Woo , Seung-Jae Lee , Hyung-Wook Kwon","doi":"10.1016/j.jinsphys.2026.104945","DOIUrl":"10.1016/j.jinsphys.2026.104945","url":null,"abstract":"<div><div>Pollinator mortality is a global concern linked to climate change; however, the complex responses underlying heat stress in honey bees are not yet fully understood. This study investigated the impact of chronic heat stress on honey bee appetitive motivation and related physiological processes that are essential for colony functioning. From June to October 2022 and April to October 2023, bees were exposed to 25°C (Group 25) and 35°C (Group 35) in controlled environments, with a Group Control kept under natural conditions. We evaluated sucrose responsiveness as an appetitive indicator, colony performance in late summer, four physiological traits, and eleven molecular markers. Nine genes were heat-sensitive, including those linked to maturity, aging (<em>AmMblk-1, Amfor</em>), and cognition (<em>JHAMT, AmOctaR1</em>). Foragers from Group 35 showed reduced sensitivity to low-sucrose solutions, potentially impairing food collection. This behavioral deficit likely contributed to poor honey stores and reduced colony development. Our findings suggest that chronic heat stress can impair honey bee foraging motivation and efficiency, posing a risk to colony health. These results underscore the broader ecological consequences of rising global temperatures for pollinators.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104945"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic depression drives discontinuous gas exchange in diapause pupae of Sericinus montelus (Lepidoptera: Papilionidae): a seasonal adaptation strategy 代谢抑制驱动孟家蚕滞育蛹不连续气体交换：一种季节性适应策略。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-02-15 DOI: 10.1016/j.jinsphys.2026.104954

Quan-Hong Xiao , Rong-Wei Wu , Zhe He , Dao-Hong Zhu

{"title":"Metabolic depression drives discontinuous gas exchange in diapause pupae of Sericinus montelus (Lepidoptera: Papilionidae): a seasonal adaptation strategy","authors":"Quan-Hong Xiao , Rong-Wei Wu , Zhe He , Dao-Hong Zhu","doi":"10.1016/j.jinsphys.2026.104954","DOIUrl":"10.1016/j.jinsphys.2026.104954","url":null,"abstract":"<div><div>Adaptive shifts in respiratory patterns during insect diapause represent a key strategy for coping with seasonal adversity, yet the underlying regulatory mechanisms remain poorly understood. Using the swallowtail butterfly Sericinus montelus Gray as a model, this study investigated the regulatory roles of diapause and temperature on respiratory patterns by comparing gas exchange patterns and metabolic characteristics of pupae under different photoperiods and temperature conditions. The results demonstrated that the metabolic rate of diapause-destined pupae was significantly lower than that of non-diapause pupae, and they stably maintained a discontinuous gas exchange (DGE) pattern. In contrast, all non-diapause individuals exhibited continuous gas exchange. Temperature influenced the respiratory pattern by modulating the metabolic rate: diapause-destined pupae maintained DGE across the entire temperature range tested, while non-diapause pupae displayed DGE at lower temperatures but switched to continuous gas exchange at higher temperatures, suggesting the existence of a metabolic rate threshold triggering the transition. Furthermore, DGE cycle parameters were regulated by both temperature and diapause duration: low temperature and diapause progression significantly extended the interburst period and the total DGE cycle duration, while the volume of CO<sub>2</sub> released per burst remained stable, which is consistent with the hygric hypothesis for DGE. This study identifies photoperiod-induced metabolic depression as the core driver of the DGE pattern in diapausing <em>S. montelus</em> pupae, with temperature fine-tuning the cycle parameters via its effect on metabolic rate. Together, these mechanisms optimize energy conservation and potentially reduce water loss, highlighting a key physiological adaptation that may vary across insect taxa.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104954"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterizations and expression profiles under temperature stress of transient receptor potential channels in Diaphorina citri 柑橘瞬时受体电位通道的分子特征及其在温度胁迫下的表达谱。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-02-20 DOI: 10.1016/j.jinsphys.2026.104959

Tian-sheng Liu , Min-Liang Bin , Hong-Yu Zhang , Gan-Jun Yi

{"title":"Molecular characterizations and expression profiles under temperature stress of transient receptor potential channels in Diaphorina citri","authors":"Tian-sheng Liu , Min-Liang Bin , Hong-Yu Zhang , Gan-Jun Yi","doi":"10.1016/j.jinsphys.2026.104959","DOIUrl":"10.1016/j.jinsphys.2026.104959","url":null,"abstract":"<div><div>Transient receptor potential (TRP) channels play critical roles in responding to external environment and regulating homeostasis in insects. TRPs have been widely investigated for their critical roles in regulating various insect behaviors in recent years, and have become potential molecular targets for insecticides or repellents. In this study, 12 TRP gene were identified and analyzed in the genome of <em>Diaphorina citri</em>, a highly destructive pest that has become a global problem due to its a vector of <em>Candidatus</em> Liberibacter asiaticus (<em>C</em>Las), the principal causal of huanglongbing (HLB). The results showed that the TRP family genes were classified into seven subfamilies in <em>D. citri</em>, including three TRPA subfamily, two TRPV and TRPC subfamily, one TRPN, TRPP, TRPM, and TRPML subfamily. Real-time quantitative polymerase chain reaction (RT-qPCR) experiments revealed widespread expression of TRP channel genes across various developmental stages and tissues of <em>D. citri</em>. The expression levels of DcTRPML, DcTRPL, and DcWtrw genes were significantly different from controls, indicating their important roles in immune responses to <em>C</em>Las infection. To investigate the potential role of TRP genes to temperature change, we detected their expression level under low and high temperature stress. The results reveal that the most of TRP genes’ transcriptional level were significantly up-regulated, suggestion their involvement in response to temperature stress in <em>D. citri</em>. Our findings provide important information and resources for developing new approaches to control ACP targeting these important ion channels.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104959"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functions of keratin-associated protein genes in reproduction of Nilaparvata lugens 角蛋白相关蛋白基因在褐飞虱生殖中的作用

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.jinsphys.2026.104947

Yi Zhang , Xinyi Zhang , Fan Zhong , Ye Han , Liwen Guan , Qixuan Mao , Liyuhan Hua , Busheng Liu , Min Zhou , Hong Yang , Yan Wu , Bin Tang

{"title":"Functions of keratin-associated protein genes in reproduction of Nilaparvata lugens","authors":"Yi Zhang , Xinyi Zhang , Fan Zhong , Ye Han , Liwen Guan , Qixuan Mao , Liyuhan Hua , Busheng Liu , Min Zhou , Hong Yang , Yan Wu , Bin Tang","doi":"10.1016/j.jinsphys.2026.104947","DOIUrl":"10.1016/j.jinsphys.2026.104947","url":null,"abstract":"<div><div>As a notorious rice pest, <em>Nilaparvata lugens</em> (Stål) (Hemiptera: Delphacidae) inflicts severe damage owing to its exceptionally high reproductive potential. Previous ovarian transcriptome analyses have revealed that the insect Keratin-associated protein (<em>KAP</em>) gene is potentially implicated in vitelline secretion and eggshell sclerotization, and it holds promise as a novel target for the suppression of pest reproduction. In this study, <em>KAP5-2</em>(LOC111045913, XP_039288177.1) and <em>KAP5-4</em> (LOC111045916, XP_022187086.1) genes’ function were studied by RNA interference technology. The results showed that gene silencing significantly suppressed female fecundity, and the pre-oviposition period extended from 3 days to 4 days. The total number of eggs laid within 10 days was significantly reduced, with only 113.2 and 101.3 eggs in the ds<em>KAP5-2</em> and ds<em>KAP5-4</em> groups, respectively, representing decreases of 49.3 % and 56.0 % compared to the control group. In addition, the hatching rates of offspring were significantly reduced by 60.1 % and 63.78 %, with many malformed unhatched eggs remaining. Molecular mechanism studies revealed that silencing <em>KAP</em> genes led to significant downregulation of vitellogenin (Vg) and its receptor (VgR, vitellogenin receptor). Additionally, it affected the expression of key genes in the juvenile hormone (Juvenile Hormone Acid Methyltransferase (<em>JHAMT)</em>, Methoprene-tolerant (<em>Met)</em>) and nutrient signaling pathways. This study indicates the critical role of <em>KAP</em> genes in regulating female reproductive processes and embryonic development in <em>N. lugens</em>, thereby providing a theoretical foundation for the rational design of eco-friendly insecticides targeting these <em>KAP</em> genes.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104947"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal signal-mediated sexual communication in parasitoids: perception, mechanism and behaviour 寄生蜂多模态信号介导的性交流：感知、机制和行为。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-02-15 DOI: 10.1016/j.jinsphys.2026.104952

Adil Tonğa , Jamin Ali , Bashiru Adams

{"title":"Multimodal signal-mediated sexual communication in parasitoids: perception, mechanism and behaviour","authors":"Adil Tonğa , Jamin Ali , Bashiru Adams","doi":"10.1016/j.jinsphys.2026.104952","DOIUrl":"10.1016/j.jinsphys.2026.104952","url":null,"abstract":"<div><div>Parasitoids mate ritualistically and rely on a combination of sensory cues to navigate sexual communication, with olfactory signals playing central roles. This review examines the ritualistic communication involved in parasitoid mating, beginning with how conspecifics detect their counterparts through chemical cues, particularly pheromones until the mate marking behaviour by males. Therefore, we aim to explore the sequential and synergistic involvement of chemical, acoustic, vibrational, visual and tactile cues in the sexual communication of parasitoids. For example, once females or males are located based on long-distance pheromonal and acoustic communication, courtship is followed by the involvement of acoustic, vibrational, contact compounds and visual signals at close-range, whereby females or males evaluate the counterpart for mate choices. The interaction between different cues during courtship and mating is examined, highlighting how they influence reproductive behaviours and success. Post-mating interactions are also discussed, focusing on how communication may continue to ensure maintenance of female reproductive strategies. This review addresses the emerging field of sexual cue perception in parasitoids, emphasizing that while initial studies have only recently begun to explore underlying mechanisms, there remains a profound lack of understanding of how parasitoid nervous systems integrate multiple sensory inputs. Emphasizing the roles of multimodal signal-mediated sexual communication in parasitoids, this review sheds light on their integrated use and suggests implications for pest management and future research directions.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104952"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omics analysis reveals AaaaNAT1 as a critical regulator of physiological homeostasis in Aedes aegypti 多组学分析显示AaaaNAT1是埃及伊蚊生理稳态的关键调节因子。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2026-03-01 Epub Date: 2026-02-19 DOI: 10.1016/j.jinsphys.2026.104956

Xue Gong , Zhinan Lin , Yanjiao Zhou , Dingfeng Duan , Qian Han , Chenghong Liao

{"title":"Multi-omics analysis reveals AaaaNAT1 as a critical regulator of physiological homeostasis in Aedes aegypti","authors":"Xue Gong , Zhinan Lin , Yanjiao Zhou , Dingfeng Duan , Qian Han , Chenghong Liao","doi":"10.1016/j.jinsphys.2026.104956","DOIUrl":"10.1016/j.jinsphys.2026.104956","url":null,"abstract":"<div><div>Physiological homeostasis is fundamental to vector competence in <em>Aedes aegypti</em>. Using RNA interference coupled with integrated multi-omics analysis (transcriptome, proteome, and metabolome), we elucidated the critical role of Arylalkylamine N-acetyltransferase-1 of <em>Aedes aegypti</em>(AaaaNAT) in maintaining salivary gland homeostasis and systemic physiological integrity. <em>AaaaNAT1</em> knockdown precipitated genome-wide transcriptomic repression in salivary glands, concurrent suppression of energy metabolism, activation of compensatory protein synthesis, and extensive metabolic reprogramming. These molecular perturbations manifested as severely compromised immune defense, evidenced by rapid mortality upon bacterial challenge, and dramatically enhanced insecticide susceptibility. Notably, the LC50 of chlorpyrifos decreased from 7.90 μg/mL to 0.93 μg/mL, representing an ∼8-fold sensitivity increase. Multi-omics integration further revealed that <em>AaaaNAT1</em> dysfunction disrupts sphingolipid signaling and impairs detoxification pathways, ultimately driving physiological collapse. Our findings establish AaaaNAT1 as a pivotal orchestrator of physiological homeostasis and stress adaptation, positioning it as a high-value molecular target for innovative vector control strategies. This work provides a robust framework for identifying critical gene targets and lays a solid foundation for translational applications in mosquito-borne disease prevention.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"169 ","pages":"Article 104956"},"PeriodicalIF":2.3,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0