Journal of insect physiology最新文献_第3页

RNAi-mediated held-out wing (HOW) gene knockdown inhibits wing expansion of white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae) rnai介导的伸出翼（hold -out wing， HOW）基因敲低抑制白背飞虱翅膀扩张

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-15 DOI: 10.1016/j.jinsphys.2025.104810

Gui-yun Long , Zhao Wang , Dao-chao Jin , Hong Yang , Sheng-jiang Yang , Cao Zhou , Qing-hui Zeng

{"title":"RNAi-mediated held-out wing (HOW) gene knockdown inhibits wing expansion of white-backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae)","authors":"Gui-yun Long , Zhao Wang , Dao-chao Jin , Hong Yang , Sheng-jiang Yang , Cao Zhou , Qing-hui Zeng","doi":"10.1016/j.jinsphys.2025.104810","DOIUrl":"10.1016/j.jinsphys.2025.104810","url":null,"abstract":"<div><div>The white-backed planthopper (<em>Sogatella furcifera</em>), a major migratory pest of rice, has resistance to various chemical treatments. The developmental regulation gene “<em>held-out wing</em> (<em>HOW</em>)” gene can serve as a potential target for RNA interference (RNAi) pesticides. Here, we analyzed spatiotemporal expression pattern and biological function of <em>SfHOW</em> by RNAi-mediated gene silencing. The <em>SfHOW</em> gene contains 628 bp and encodes 196 amino acids. <em>SfHOW</em> was highly expressed in 3<sup>rd</sup> instar nymphs, followed by 5<sup>th</sup> instar nymphs. In different tissues, <em>SfHOW</em> is most abundantly expressed in epidermis, with lower levels in wings and legs, and the least expression observed in fat body and gut. RNAi-mediated silencing of <em>SfHOW</em> resulted in a marked decrease in its transcription levels, leading to mortality of 66.8 %. Additionally, 62.0 % emerged adults exhibited folded and curled wings, indicating that <em>SfHOW</em> is crucial for wing expansion of <em>S. furcifera</em>. Following <em>SfHOW</em> silencing, significant reductions in the expression of <em>S. furcifera Apterous</em> (<em>SfAp</em>), suggesting that <em>SfHOW</em> may regulate wing expansion by modulating <em>SfAp,</em> so as to regulate the expression of <em>Dpp</em> gene to participate in the regulation of expansion. This study identified a new target for the development of RNAi-based pesticides for rice pest control and enhances molecular understanding of wing development in Hemipteran insects.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104810"},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue- and temperature-dependent expression, enzyme activity, and RNAi knockdown of Catalase in a freeze-tolerant insect 耐冻昆虫过氧化氢酶的组织和温度依赖性表达、酶活性和RNAi敲除

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-11 DOI: 10.1016/j.jinsphys.2025.104809

Sarah E. Rokosh, Victoria E. Adams, Robyn Walter , Grace E. Kaiser, Amber L. Gough, Jantina Toxopeus

{"title":"Tissue- and temperature-dependent expression, enzyme activity, and RNAi knockdown of Catalase in a freeze-tolerant insect","authors":"Sarah E. Rokosh, Victoria E. Adams, Robyn Walter , Grace E. Kaiser, Amber L. Gough, Jantina Toxopeus","doi":"10.1016/j.jinsphys.2025.104809","DOIUrl":"10.1016/j.jinsphys.2025.104809","url":null,"abstract":"<div><div>Organisms that overwinter in temperate climates may experience freezing and freezing-induced oxidative stress during winter. While many insect species can survive freezing, reverse genetics techniques such as RNA interference (RNAi) have not been used to understand the physiological mechanisms underlying freeze tolerance. The spring field cricket <em>Gryllus veletis</em> can survive freezing following a 6-week fall-like acclimation. We used RNAi to knock down expression of an antioxidant enzyme in <em>G. veletis</em> to test the hypothesis that minimizing oxidative stress is important for freeze tolerance. In fat body tissue, <em>Catalase</em> mRNA abundance and enzyme activity increased during the fall-like acclimation that induces freeze tolerance. Other tissues such as midgut and Malpighian tubules had more stable or lower <em>Catalase</em> expression and activity during this acclimation. In summer-acclimated (freeze-intolerant) crickets, RNA interference (RNAi) effectively knocked down production of the <em>Catalase</em> mRNA and protein in fat body and midgut, but not Malpighian tubules. In fall-acclimated (freeze-tolerant) crickets, RNAi efficacy was temperature-dependent, functioning well at warm (c. 22 °C) but not cool (15 °C or lower) temperatures. This highlights a challenge of using RNAi in organisms acclimated to low temperatures, as they may need to be warmed up for RNAi to work, potentially affecting their stress physiology. Knockdown of <em>Catalase</em> via RNAi in fall-acclimated crickets also had no effect on the ability of the crickets to survive a mild freeze treatment, suggesting that Catalase may not be necessary for freeze tolerance. Our study is the first to demonstrate that RNAi is possible in a freeze-tolerant insect, but further research is needed to examine whether other genes and antioxidants are needed for <em>G. veletis</em> freeze tolerance.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104809"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive transcriptome and metabolome analysis of the adaptability and detoxification ability of Spodoptera frugiperda larvae to tobacco 狐尾夜蛾幼虫对烟草的适应性和解毒能力的综合转录组和代谢组分析

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-11 DOI: 10.1016/j.jinsphys.2025.104800

Minghui Xie , Yongzhi Zhong , Lulu Lin , Guangling Zhang , Ning Wei , Feng Zhang , Haoliang Chen

{"title":"Comprehensive transcriptome and metabolome analysis of the adaptability and detoxification ability of Spodoptera frugiperda larvae to tobacco","authors":"Minghui Xie , Yongzhi Zhong , Lulu Lin , Guangling Zhang , Ning Wei , Feng Zhang , Haoliang Chen","doi":"10.1016/j.jinsphys.2025.104800","DOIUrl":"10.1016/j.jinsphys.2025.104800","url":null,"abstract":"<div><div><em>Spodoptera frugiperda</em> is among the most significant pests affecting food crops globally. Our findings indicate that the mortality rates of 1st and 2nd instar larvae are significantly higher than those of 3rd instar and older larvae following tobacco consumption. In this study, we employed integrated transcriptomic and metabolomic analyses to investigate the changes in gene expression and metabolic processes in 2nd and 3rd instar larvae after consuming tobacco and maize. Transcriptome analysis revealed that the majority of differentially expressed genes (DEGs) involved in xenobiotic biodegradation and metabolism were upregulated, particularly cytochrome P450s. Metabolomic analysis identified alkaloid metabolites in the bodies of larvae that had fed on tobacco. The 3rd instar larvae that consumed tobacco exhibited increased production of metabolites via cytochrome P450. Correlation analysis of the transcriptome and metabolome demonstrated that, when comparing the 3rd instar larvae fed on tobacco to those fed on maize, both DEGs and differentially accumulated metabolites (DAMs) shared pathways related to cytochrome P450, fatty acid metabolism, and glutathione metabolism. Transcriptome and metabolome analysis shows cytochrome P450 play an important role in the detoxification and adaptability of <em>S. frugiperda</em> larvae to tobacco. This study provides a preliminary explanation of the detoxification metabolism and adaptive mechanisms of <em>S. frugiperda</em> larvae in response to tobacco.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104800"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of successful or unsuccessful parasitism by the solitary endoparasitoid wasp Cotesia vestalis (Hymenoptera: Braconidae) on the testis development in several host moth species 膜翅目：小茧蜂（Cotesia vestalis）寄生成功或不成功对几种寄主蛾睾丸发育的影响

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-11 DOI: 10.1016/j.jinsphys.2025.104808

Satoshi Hiroyoshi , Takayuki Mitsunaga , Gadi V.P. Reddy

{"title":"Effects of successful or unsuccessful parasitism by the solitary endoparasitoid wasp Cotesia vestalis (Hymenoptera: Braconidae) on the testis development in several host moth species","authors":"Satoshi Hiroyoshi , Takayuki Mitsunaga , Gadi V.P. Reddy","doi":"10.1016/j.jinsphys.2025.104808","DOIUrl":"10.1016/j.jinsphys.2025.104808","url":null,"abstract":"<div><div>Parasitism by a parasitoid wasp alters the physiology and behavior of its host insects. This study investigated the effects of successful and unsuccessful parasitization on individual host development and testis growth in several moth species. Although the solitary koinobiont endoparasitoid wasp, <em>Cotesia vestalis</em> (Hymenoptera: Braconidae), primarily parasitizes the diamondback moth (<em>Plutella xylostella</em>, Lepidoptera: Plutellidae), its host range is relatively broad. Previous research confirmed successful parasitization of <em>P. xylostella</em>, <em>Mythimna separata</em> (Noctuidae), and <em>Helicoverpa armigera armigera</em> (Noctuidae), whereas attempts to parasitize <em>Spodoptera exigua</em> (Noctuidae), <em>Palpita nigropunctalis</em> (Piralidae), <em>Aedia leucomelas</em> (Nocuidae), and <em>Mamestra brasiccae</em> (Noctuidae) were unsuccessful. In this study, testis development in all parasitized hosts, except for <em>A</em>. <em>leucomelas</em>. was suppressed or reduced compared to the unparasitized controls. Notably, testes in parasitized hosts exhibited some degree of growth after parasitization—varying by species and occurring independently of ecdysis—except in final instar larvae of <em>P. xylostella</em>. These finding suggest that parasitism suppresses or reduce testis development regardless of whether parrasistization is successful. The potential mechanisms underlying a reduction of testis development in unsuccessful parasitization are discussed in the context of ecdysteroid signaling and the parasitic process.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104808"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A C-type lectin of Helicoverpa armigera maintains the stability of the hemolymph microbiota by regulating the expression of lysozyme 棉铃虫c型凝集素通过调节溶菌酶的表达来维持血淋巴微生物群的稳定性。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-04 DOI: 10.1016/j.jinsphys.2025.104799

Guijie Wang , Jialin Wang , Xusheng Liu

{"title":"A C-type lectin of Helicoverpa armigera maintains the stability of the hemolymph microbiota by regulating the expression of lysozyme","authors":"Guijie Wang , Jialin Wang , Xusheng Liu","doi":"10.1016/j.jinsphys.2025.104799","DOIUrl":"10.1016/j.jinsphys.2025.104799","url":null,"abstract":"<div><div>An increasing body of evidence suggests that the insect hemolymph is not a sterile environment and that various nonpathogenic microorganisms can stably or transiently inhabit the hemolymph in many insect species. However, little is currently known about how the insect immune system maintains microbial homeostasis within the hemolymph. In this study, a C-type lectin of <em>Helicoverpa armigera</em> (HaCTL6) was shown to be involved in maintaining the stability of the hemolymph microbiota. The expression of <em>H. armigera</em> antimicrobial peptide (AMP) genes was down-regulated after RNAi of HaCTL6. Moreover, the knockdown of HaCTL6 resulted in a decrease in the antibacterial activity and an increase in the total bacterial load of the hemolymph. Transcriptome analysis showed that a lysozyme (HaLysozyme-like) was significantly down-regulated after HaCTL6 RNAi. Moreover, the knockdown of HaLysozyme-like led to a decrease in the antibacterial activity and an increase in the total bacterial load of the hemolymph. Furthermore, the injection of recombinant HaLysozyme-like into the hemocoel caused a significant reduction in the total number of bacteria in the hemolymph. These results indicate that HaCTL6 may regulate the homeostasis of bacteria in the hemolymph by utilizing HaLysozyme-like as an effector.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"163 ","pages":"Article 104799"},"PeriodicalIF":2.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143795655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for a command neuron controlling calling song in the cricket Gryllus assimilis 蟋蟀体内控制鸣叫的指令神经元的证据

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-01 DOI: 10.1016/j.jinsphys.2025.104798

Chu-Cheng Lin, Berthold Hedwig

{"title":"Evidence for a command neuron controlling calling song in the cricket Gryllus assimilis","authors":"Chu-Cheng Lin, Berthold Hedwig","doi":"10.1016/j.jinsphys.2025.104798","DOIUrl":"10.1016/j.jinsphys.2025.104798","url":null,"abstract":"<div><div>In invertebrates stereotypical behaviours may be controlled by command neurons. In the field cricket <em>Gryllus bimaculatus</em> a command neuron descending from the brain controls the generation of rhythmic wing movements underlying calling song. We analysed if a corresponding command neuron also exists in other cricket species. Our intracellular recording and stimulation experiments point to a putative command neuron for calling song in <em>G. assimilis</em>, although the structure of the neuron could not be revealed. When this neuron was depolarised to generate 50 AP/s the cricket raised its forewings into singing position, it started rhythmic wing movements and produced the typical species-specific calling song. Further enhancing the spike rate of the neuron increased the chirp repetition rate but not the pulse repetition rate. Blocking the spike activity of the neuron by hyperpolarizing current injection reduced the chirp repetition rate and could terminate singing activity. Our evidence indicates that the two species, <em>G. assimilis</em> and <em>G. bimaculatus</em>, have homologous neurons for controlling calling song, which may be a conserved phenotype across cricket species.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104798"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein-rich trophallactic contents transferred from reproductives are crucial for termite soldier differentiation in Zootermopsis nevadensis 从生殖体转移的富含蛋白质的营养物是内华达虫病白蚁士兵分化的关键。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-04-01 DOI: 10.1016/j.jinsphys.2025.104797

Hajime Yaguchi , Makoto Matsushita , Kiyoto Maekawa

{"title":"Protein-rich trophallactic contents transferred from reproductives are crucial for termite soldier differentiation in Zootermopsis nevadensis","authors":"Hajime Yaguchi , Makoto Matsushita , Kiyoto Maekawa","doi":"10.1016/j.jinsphys.2025.104797","DOIUrl":"10.1016/j.jinsphys.2025.104797","url":null,"abstract":"<div><div>Polyphenic castes exist for colony success in social animals. A primer pheromone has been suggested as a well-known regulator of caste development, but the basis of chemical communication has remained elusive over the last few decades. In termites, a long-standing hypothesis is that reproductive-secreted juvenile hormone (JH) plays a role as a primer pheromone involved in soldier differentiation. Here, we tested whether exogenous JH is necessary to induce differentiation of soldiers in the dampwood termite <em>Zootermopsis nevadensis</em>. The unique characteristic of this termite is that soldier-destined individuals can be monitored during their ontogeny under natural conditions. Furthermore, oral–anal trophallactic interactions with reproductives are required for soldier differentiation. First, knockdown of JH biosynthetic genes using RNA interference (RNAi) resulted in the reduction of soldier formation but did not affect the frequency of trophallactic interaction from reproductives to RNAi-treated individuals. Next, we compared the effect of parental replacement on soldier differentiation. Old-age reproductives had fewer stimulus effects on the differentiation of soldier-destined individuals compared to young-age reproductives. This difference in stimulus effects was derived from the protein levels within the digestive fluids of the king, but not those of the queen. Consequently, there is little or no possibility that JH itself plays a role as a primer pheromone. Alternatively, we propose that protein-rich nutrients in trophallactic fluids from reproductives are sufficient to induce high JH levels in larvae required for soldier differentiation.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104797"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusion of silkworm polar body nuclei 蚕极体核的融合

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-03-27 DOI: 10.1016/j.jinsphys.2025.104795

Hiroki Sakai , Shuichiro Tomita , Takeshi Yokoyama

{"title":"Fusion of silkworm polar body nuclei","authors":"Hiroki Sakai , Shuichiro Tomita , Takeshi Yokoyama","doi":"10.1016/j.jinsphys.2025.104795","DOIUrl":"10.1016/j.jinsphys.2025.104795","url":null,"abstract":"<div><div>Polar bodies are produced during the development of oocytes. In many animals, polar bodies extrude from the egg and typically degenerate after the meiotic maturation of the oocyte. However, in insects, polar body nuclei remain within the egg and neither extrude nor degenerate during early embryogenesis. Previously, we reported that in the silkworm (<em>Bombyx mori</em>), the polar body nuclei differentiate into serosa cells. In this study, we observed the behavior of polar body nuclei during early embryogenesis using a tissue clearing method. Individuals with a reduced number of polar body nuclei were observed around 4–6 h after egg laying during incubation at 25℃, and after 6 h, the polar body nuclei were observed to have increased in number in some individuals. Analysis using serosa cell mutants (<em>pe</em> and <em>re</em>) revealed that most of the polar body-derived serosa cells had undergone fusion of the polar body nuclei. These findings indicate that in silkworms, polar body nuclei fuse approximately 4–6 h after egg laying, which corresponds to the cleavage stage, during which fertilized nuclei divide into several to dozens of nuclei. In some individuals, the fused polar body nuclei divide, increase in number, and differentiate into serosa cells.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104795"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of mating-related behavior by serotonin in males of the Japanese yellow hornet Vespa simillima 5 -羟色胺对雄性日本黄黄蜂交配相关行为的激活作用

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-03-27 DOI: 10.1016/j.jinsphys.2025.104796

Tomohiro Watanabe , Takafumi Onuma , Masakazu Nishimura , Ayaka Morigami , Masato Ono , Ken Sasaki

{"title":"Activation of mating-related behavior by serotonin in males of the Japanese yellow hornet Vespa simillima","authors":"Tomohiro Watanabe , Takafumi Onuma , Masakazu Nishimura , Ayaka Morigami , Masato Ono , Ken Sasaki","doi":"10.1016/j.jinsphys.2025.104796","DOIUrl":"10.1016/j.jinsphys.2025.104796","url":null,"abstract":"<div><div>The behavioral physiology of males in social wasps has received little attention, despite the importance of male behavior adapted to complex social environments. To explore the roles of brain biogenic amines in mating-related behavior in male Japanese yellow hornets (<em>Vespa simillima</em>), we first determined the development of mating-related behavior and age-related changes to biogenic amines in the brain. The activities of locomotion, flight, and mating in the males increased with day-age by 1 week after emergence. Testes size decreased within 1 week after adult emergence, suggesting that male sexual maturation may be complete with the development of mating-related behavior. Serotonin levels in the brain increased with age in parallel to the behavioral activities. Dopamine levels in the brain peaked at 2 days of age and then decreased with age, whereas octopamine levels in the brain decreased with age. Thus, serotonin was a candidate compound activating mating-related behavior in males. We then examined the effects of serotonin on the mating-related behavior of males using serotonin injections. Injections of serotonin significantly enhanced activities of locomotion, flight, and mating, depending on serotonin concentration. Those results suggested that serotonin activates mating-related behavior in male hornets.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104796"},"PeriodicalIF":2.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockdown of a chemosensory protein disrupts soil-guided behavior of a subterranean larval pest 化学感觉蛋白的敲除破坏了地下害虫幼虫的土壤引导行为。

IF 2.3 2区农林科学

Journal of insect physiology Pub Date : 2025-03-15 DOI: 10.1016/j.jinsphys.2025.104793

Paula Lizana , Camila Muñoz , Ana Mutis , Leonardo Velasco , Rubén Palma-Millanao , Andrés Quiroz , Herbert Venthur

{"title":"Knockdown of a chemosensory protein disrupts soil-guided behavior of a subterranean larval pest","authors":"Paula Lizana , Camila Muñoz , Ana Mutis , Leonardo Velasco , Rubén Palma-Millanao , Andrés Quiroz , Herbert Venthur","doi":"10.1016/j.jinsphys.2025.104793","DOIUrl":"10.1016/j.jinsphys.2025.104793","url":null,"abstract":"<div><div>In recent years, RNA interference (RNAi) has become a widely studied tool for the functional analysis of genes and more recently, for pest control. <em>Hylamorpha elegans</em> (Coleoptera: Scarabaeidae) is a beetle endemic to Chile, considered an important pest during its larval stage as white grubs, feeding on organic matter (OM) and crop roots (e.g., wheat and red clover). Its control is limited due to its subterranean behavior. Thus, studying a chemosensory system as a fundamental part of the transport and recognition of chemicals from the environment could provide new targets for the knowledge and control of this beetle. Recently, chemosensory genes have been identified for <em>H. elegans</em> white grubs, and a chemosensory protein (CSP) was selected. This study aimed to evaluate the functional role of a highly expressed CSP in the subterranean behavior of white grubs. For this purpose, food preference assays were performed and standardized. Afterward, double-stranded RNA (dsRNA) was synthesized based on a selected CSP. A chosen preference assay was conducted using white grubs treated with dsRNA. Findings showed that white grubs prefer peat with high OM over red clover roots. Additionally, the CSP gene is upregulated when OM content increases. Finally, the knockdown of the CSP led to a disruption in soil-guided behavior. This protein may represent a novel target to be studied in the frame of management strategies for <em>H. elegans</em>.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"162 ","pages":"Article 104793"},"PeriodicalIF":2.3,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0