Journal of insect physiology最新文献

{"title":"No evidence for the melanin desiccation hypothesis in a larval Lepidopteran","authors":"Sarah Britton ,&nbsp;Goggy Davidowitz","doi":"10.1016/j.jinsphys.2024.104669","DOIUrl":"10.1016/j.jinsphys.2024.104669","url":null,"abstract":"<div><p>Water regulation is an important physiological challenge for insects due to their small body sizes and large surface area to volume ratios. Adaptations for decreasing cuticular water loss, the largest avenue of loss, are especially important. The melanin desiccation hypothesis states that melanin molecules in the cuticle may help prevent water loss, thus offering protection from desiccation. This hypothesis has much empirical support in <em>Drosophila</em> species, but remains mostly untested in other taxa, including Lepidoptera. Because melanin has many other important functions in insects, its potential role in desiccation prevention is not always clear. In this study we investigated the role of melanin in desiccation prevention in the white-lined Sphinx moth, <em>Hyles lineata</em> (Lepidoptera, Sphingidae), which shows high plasticity in the degree of melanin pigmentation during the late larval instars. We took advantage of this plasticity and used density treatments to induce a wide range of cuticular melanization; solitary conditions induced low melanin pigmentation while crowded conditions induced high melanin pigmentation. We tested whether more melanic larvae from the crowded treatment were better protected from desiccation in three relevant responses: <em>i)</em> total water loss over a desiccation period, <em>ii)</em> change in hemolymph osmolality over a desiccation period, and <em>iii)</em> evaporation rate of water through the cuticle. We did not find support for the melanin desiccation hypothesis in this species. Although treatment influenced total water loss, this effect did not occur via degree of melanization. Interestingly, this implies that crowding, which was used to induce high melanin phenotypes, may have other physiological effects that influence water regulation. There were no differences between treatments in cuticular evaporative water loss or change in hemolymph osmolality. However, we conclude that osmolality may not sufficiently reflect water loss in this case. This study emphasizes the context dependency of melanin’s role in desiccation prevention and the importance of considering how it may vary across taxa. In lepidopteran larvae that are constantly feeding phytophagous insects with soft cuticles, melanin may not be necessary for preventing cuticular water loss.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An improved method to assess the encapsulation response in arthropods","authors":"Lucas J. Kirschman,&nbsp;Hannah M. Eastman,&nbsp;Frank J. Irovic,&nbsp;Nathaniel A. Nix,&nbsp;Ly Tuan Kiet Bui,&nbsp;Sydney L. Blackmon,&nbsp;Jaylen S. Greenlee,&nbsp;Rubina Lamichhane,&nbsp;Jack D. Mabuce,&nbsp;Hannah K. McAllister,&nbsp;Lilly F. Nevill,&nbsp;Peyton Redinger,&nbsp;Nia I. Rivers,&nbsp;Jackson L. Sprague","doi":"10.1016/j.jinsphys.2024.104670","DOIUrl":"10.1016/j.jinsphys.2024.104670","url":null,"abstract":"<div><p>Ecoimmunology explores how ecological factors and evolutionary processes influence immune responses across various taxa and how immune responses trade-off with other traits. Studying immune responses requires biologically meaningful immunoassays applicable to a broad range of taxa and are sensitive enough to detect changes in the immune response. Useful immunoassays should also correlate with immunocompetence and fitness. The encapsulation response, a complex immune mechanism in arthropods, serves as a robust method for ecoimmunological investigations. However, traditional methods to test the encapsulation response can require long training. This study introduces an innovative, cost-effective method for assessing the encapsulation immune response in arthropods, which simplifies the procedure by reducing the training time and skill required. Our modified device utilizes a pen and syringe assembly for inserting monofilaments into arthropod larvae. We compared our device against traditional methods. Despite the new method being 22% faster, it did not compromise the accuracy or effectiveness of the encapsulation response when compared with traditional techniques, demonstrating similar degrees of melanization and encapsulation. Our method allowed for more accessible participation by less experienced researchers, such as undergraduates, facilitating their involvement in ecoimmunological research.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caste-specific development of the dopaminergic system in bumble bees (Bombus ignitus)","authors":"Takafumi Onuma,&nbsp;Ken Sasaki","doi":"10.1016/j.jinsphys.2024.104665","DOIUrl":"10.1016/j.jinsphys.2024.104665","url":null,"abstract":"<div><p>The dopaminergic system is involved in caste-specific behaviors in eusocial bumble bees. However, little is known about how the caste differences in dopaminergic system are formed during pupal stages in the brains of bumble bees. Thus, we investigated the levels of dopamine-related substances and expression of genes encoding enzymes involved in dopamine synthesis and metabolism, dopamine receptors, and a dopamine transporter in the brain of female <em>Bombus ignitus</em>. The levels of dopamine and dopamine-related substances in the brain were significantly higher in gynes than in workers from the late pupal stage to emergence, but the dynamics were similar between the castes. The relative expression levels of genes encoding enzymes involved in dopamine synthesis (<em>BigTh</em> and <em>BigDdc</em>) and dopamine metabolism (<em>BigNat</em>) increased significantly from pupal stage to emergence, but there were no differences in the relative expression levels of these genes between castes. A similar pattern was seen in the relative expression levels of four dopamine receptor genes (<em>BigDop1</em>, <em>BigDop2</em>, <em>BigDop3</em>, and <em>BigDopEcR</em>) and a dopamine transporter gene (<em>BigDat</em>). Compared with the honey bee <em>Apis mellifera</em>, the caste-specific dopaminergic system in the bumble bee is less differentiated, which might reflect the degree of behavioral specialization in these two species.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141436945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A sperm-activating trypsin-like protease from the male reproductive tract of Spodoptera litura: Proteomic identification, sequence characterization, gene expression profile, RNAi and the effects of ionizing radiation","authors":"Priya Yadav ,&nbsp;Rakesh K. Seth ,&nbsp;Stuart E. Reynolds","doi":"10.1016/j.jinsphys.2024.104664","DOIUrl":"10.1016/j.jinsphys.2024.104664","url":null,"abstract":"<div><p>Like other lepidopteran insects, males of the tobacco cutworm moth, <em>Spodoptera litura</em> produce two kinds of spermatozoa, eupyrene (nucleate) and apyrene (anucleate) sperm. Formed in the testis, both kinds of sperm are released into the male reproductive tract in an immature form and are stored in the duplex region of the tract. Neither type of sperm is motile at this stage. When stored apyrene sperm from the duplex are treated <em>in vitro</em> with an extract of the prostatic region of the male tract, or with mammalian trypsin, they become motile; activation is greater and achieved more rapidly with increasing concentration of extract or enzyme. The activating effect of prostatic extract is blocked by soybean trypsin inhibitor (SBTI), also in a dose-dependent way. These results suggest that the normal sperm-activating process is due to an endogenous trypsin-like protease produced in the prostatic region.</p><p>Proteomic analysis of <em>S. litura</em> prostatic extracts revealed a <u>T</u>rypsin-<u>L</u>ike <u>S</u>erine <u>P</u>rotease, TLSP, molecular weight 27 kDa, whose 199-residue amino acid sequence is identical to that of a predicted protein from the <em>S. litura</em> genome and is highly similar to predicted proteins encoded by genes in the genomes of several other noctuid moth species. Surprisingly, TLSP is only distantly related to Serine Protease 2 (initiatorin) of the silkmoth, <em>Bombyx mori</em>, the only identified lepidopteran protein so far shown to activate sperm. TLSP has features typical of secreted proteins, probably being synthesized as an inactive precursor zymogen, which is later activated by proteolytic cleavage.</p><p>cDNA was synthesized from total RNA extracted from the prostatic region and was used to examine TLSP expression using qPCR. <em>tlsp</em> mRNA was expressed in both the prostatic region and the accessory glands of the male tract. Injection of TLSP-specific dsRNA into adult males caused a significant reduction after 24 h in <em>tlsp</em> mRNA levels in both locations. The number of eggs laid by females mated to adult males that were given TLSP dsRNA in 10 % honey solution, and the fertility (% hatched) of the eggs were reduced. Injecting pupae with TLSP dsRNA caused the later activation of apyrene sperm motility by adult male prostatic extracts to be significantly reduced compared to controls.</p><p>Exposure of <em>S. litura</em> pupae to ionizing radiation significantly reduced expression of <em>tlsp</em> mRNA in the prostatic part and accessory gland of irradiated males in both the irradiated generation and also in their (unirradiated) F1 progeny. The implications of these findings for the use of the inherited sterility technique for the control of <em>S. litura</em> and other pest Lepidoptera are discussed.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0022191024000520/pdfft?md5=aaf7d6049ff86d7a0e278e7f9df29b02&pid=1-s2.0-S0022191024000520-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Basking improves but winter warming worsens overwinter survival in the linden bug","authors":"Jan Rozsypal","doi":"10.1016/j.jinsphys.2024.104655","DOIUrl":"10.1016/j.jinsphys.2024.104655","url":null,"abstract":"<div><p>The present study investigates the effects of rare winter basking behavior (observed in wild populations of the Linden bug, <em>Pyrrhocoris apterus</em>) and the effects of winter warming (predicted by climate models) on overwinter survival and physiology of <em>P. apterus</em>. The insects were exposed to scenarios simulating basking and winter warming in the laboratory. Part of the insects were exposed to real winters under semi-natural conditions in the field for comparison. The results show a clear positive effect of winter basking, implying that basking behavior is critical for overwinter survival in <em>P. apterus</em>. In contrast, winter warming was found to have a strong negative effect on overwinter survival, potentially representing a threat to central European populations of <em>P. apterus</em>. Physiological parameters (mass, water content, SCP, energy reserves) measured in this study cannot fully explain all the results. Further study is needed to better understand the mechanisms behind the positive effects of winter basking and the negative effects of winter warming on overwintering <em>P. apterus</em>.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of temperature on the photoperiodic time measurement and on the induction of Trichogramma telengai progeny diapause: The separation of the two effects","authors":"Sergey Ya. Reznik,&nbsp;Natalia D. Voinovich","doi":"10.1016/j.jinsphys.2024.104654","DOIUrl":"https://doi.org/10.1016/j.jinsphys.2024.104654","url":null,"abstract":"<div><p>Thermal effects on photoperiodic time measurement and accumulation of inductive photoperiods have been studied in many insect species whereas the influence of temperature on the last step of the photoperiodic response, the induction of diapause, received less attention from researchers. We investigated thermal modification of the maternal photoperiodic response in <em>Trichogramma telengai</em> (Hymenoptera: Trichogrammatidae). Even a single long-night photoperiod experienced by females of this minute egg parasitoid immediately before oviposition causes a substantial increase in larval diapause incidence in the progeny. This feature allows separation of the thermal effects on different steps of the diapause-inducing photoperiodic response. Laboratory experiments showed that the temperature of the last scotophase (when the final decisive photoperiodic time measurement occurs) caused an inverted U-shaped diapause-inducing response similar to that observed in some other long-day insects. The temperature of the last photophase (when progeny diapause is induced) had a positive linear effect that has not been reported for the induction of winter diapause in any long-day insect. Most probably, such a thermal response is not a specific seasonal adaptation but a direct consequence of the influence of temperature on the rate of metabolism.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141095395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Habituation leads to short but not long term memory formation in mosquito larvae","authors":"Martin Dessart,&nbsp;Claudio R. Lazzari,&nbsp;Fernando J. Guerrieri","doi":"10.1016/j.jinsphys.2024.104650","DOIUrl":"10.1016/j.jinsphys.2024.104650","url":null,"abstract":"<div><p>In animals, memory allows to remember important locations and conserve energy by not responding to irrelevant stimuli. However, memory formation and maintenance are metabolically costly, making it worthwhile to understand the mechanisms underlying different types of memory and their adaptive value. In this study, we investigated the memory persistence of <em>Aedes aegypti</em> mosquito larvae, after habituation to a visual stimulus. We used an automated tracking system for quantifying the response of mosquito larvae to the passage of a shadow, simulating an approaching predator. First, we compared different retention times, from 4 min to 24 h, and found that mosquito larvae only exhibited memory capabilities less than 3 h after training. Secondly, we investigated the role of inter-trial intervals in memory formation. In contrast to other aquatic invertebrates, mosquito larvae showed no long-term memory even at long inter-trial intervals (i.e., 5 min and 10 min). Our results are discussed in relation to the ecological constraints.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracheal hyperallometry and spatial constraints in a large beetle","authors":"Tomer Urca ,&nbsp;Gal Ribak ,&nbsp;Eran Gefen","doi":"10.1016/j.jinsphys.2024.104652","DOIUrl":"10.1016/j.jinsphys.2024.104652","url":null,"abstract":"<div><p>Insects exchange respiratory gases with their environment through their gas-filled tracheal system, a branched tracheal tree extending from segmental openings and terminating at fine tissue penetrating tracheoles. It was shown that the tracheal volume increases hyperallometrically with insect body size (<span><math><mrow><msub><mi>M</mi><mi>b</mi></msub></mrow></math></span>), both interspecifically and across developmental stages. In this study, we used the sixfold <span><math><mrow><msub><mi>M</mi><mi>b</mi></msub></mrow></math></span> variation in adult <em>Batocera rufomaculata</em> <!-->(Cerambicidae; Coleoptera) examining the allometry of adult tracheal volume (<span><math><mrow><msub><mi>V</mi><mrow><mi>tr</mi></mrow></msub></mrow></math></span>). We further explored the effect of sex and sexual maturity on tracheal gas conductance, testing the hypotheses that (i) larger body size and (ii) egg volume in gravid females would result in lower safety margins for tracheal oxygen transport due to structural restriction. We report a hyperallometric tracheal growth in both sexes of adult <em>B. rufomaculata</em> <!-->(mean mass exponent of 1.42 ± 0.09), similar in magnitude to previously reported values. Tracheal gas conductance was independent of <span><math><mrow><msub><mi>M</mi><mi>b</mi></msub></mrow></math></span> and reproductive state, but was significantly higher in females compared with males. We suggest that females may have pre-adapted a higher tracheal conductance required for the higher flight power output while gravid. Lack of compliant air sacs and rigid trachea may explain how gravid females retain their <span><math><mrow><msub><mi>V</mi><mrow><mi>tr</mi></mrow></msub></mrow></math></span>. However, we show that <span><math><mrow><msub><mi>V</mi><mrow><mi>tr</mi></mrow></msub></mrow></math></span> outgrows thoracic dimensions with increased <em>B. rufomaculata</em> <!-->size. Hyperallometric growth of the giant cerambycid thoracic trachea could explain the previously reported hypometric scaling of flight muscles in <em>B. rufomaculata</em>, and the compromised long-distance flight performance of larger compared with smaller conspecifics.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141080632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemolin increases the immune response of a caterpillar to NPV infection","authors":"Liqiong Yan,&nbsp;Arina Nur Faidah,&nbsp;Lili Sun,&nbsp;Chuanwang Cao","doi":"10.1016/j.jinsphys.2024.104651","DOIUrl":"10.1016/j.jinsphys.2024.104651","url":null,"abstract":"<div><p>Hemolin, a member of the immunoglobulin superfamily, plays a crucial role in the immune responses of insects against pathogens. However, the innate immune response of Hemolin to baculovirus infection varies among different insects, and the antiviral effects of Hemolin in <em>Hyphantria cunea</em> (HcHemolin) remain poorly understood. Our results showed that <em>HcHemolin</em> was expressed throughout all developmental stages, with higher expressions observed during pupal and adult stages of <em>H. cunea</em>. Additionally, <em>HcHemolin</em> was expressed in reproductive and digestive organs. The expression levels of the <em>HcHemolin</em> were induced significantly following <em>H. cunea</em> nucleopolyhedrovirus (HcNPV) infection. The susceptibility of <em>H. cunea</em> larvae to HcNPV decreased upon silencing of <em>HcHemolin</em>, resulting in a 40% reduction in median lifespan compared to the control group. The relative growth rate (RGR), the relative efficiency of consumption rate (RCR), the efficiency of the conversion of ingested food (ECI), and efficiency of the conversion of digested food (ECD) of silenced <em>H. cunea</em> larvae were significantly lower than those of the control group. Immune challenge assays showed that the median lifespan of treated <em>H. cunea</em> larvae was two-fold longer than the control group after HcNPV and HcHemolin protein co-injection. Therefore, we propose that HcHemolin plays a crucial role in regulating the growth, development, and food utilization of <em>H. cunea</em>, as well as in the antiviral immune response against HcNPV. These findings provide implications for the development of targeted nucleic acid pesticides and novel strategies for pollution-free biological control synergists for HcNPV.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of climate change on the reproductive diapause and voltinism of the carrot weevil, Listronotus oregonensis","authors":"Annie-Ève Gagnon,&nbsp;Gaétan Bourgeois","doi":"10.1016/j.jinsphys.2024.104653","DOIUrl":"10.1016/j.jinsphys.2024.104653","url":null,"abstract":"<div><p>The impacts of climate change on the development of insects are of great concern due to potential alterations in population dynamics and pest pressure. The carrot weevil, <em>Listronotus oregonensis</em>, is a major agricultural pest, and its development is influenced by temperature and photoperiod. In this study, our aim was to investigate the impact of temperature increases on the voltinism and reproductive diapause of the carrot weevil under field conditions and bioclimatic models. Field observations were conducted over two growing seasons using structures that allowed for temperature increases. The developmental stages of the carrot weevil, including female reproductive status, oviposition and larval stage, were monitored weekly to measure the proportion of individuals undergoing an additional generation. Concurrently, bioclimatic models were used to simulate the probability of a second generation under current (1981–2010) and future (2041–2070) climates, considering a lower and a higher change in emission scenarios. Results showed that rising temperatures led to an increase in the proportion of carrot weevils undergoing inhibition of the reproductive diapause and a higher number of eggs laid in the field. The models indicated a substantial rise in the probability of a second generation developing, from 24% to 37% to 62%–99% under current and future climates, respectively. These findings demonstrate the potential for significant alterations in carrot weevil population dynamics, resulting in increased pest pressure on crops. Further research is needed to fully understand the implications of these findings and to develop effective adaptation measures to mitigate the negative impacts of global warming on insect populations and agriculture.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141054209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}