Journal of insect physiology最新文献

{"title":"Changes in the apolipophorin III in Galleria mellonella larvae treated with Pseudomonas aeruginosa exotoxin A.","authors":"Bartłomiej Iwański, M. Andrejko","doi":"10.2139/ssrn.4381128","DOIUrl":"https://doi.org/10.2139/ssrn.4381128","url":null,"abstract":"In the present study, we have demonstrated a correlation in time between changes in the amount of apolipophorin III (apoLp-III) in the fat body and hemocytes of Galleria mellonella larvae challenged with Pseudomonas aeruginosa exotoxin A (exoA). An increase in the amount of apoLp-III was detected 1-8 h after the challenge; then, a temporary decrease was observed after 15 h followed by an increase in the level of apoLp-III, however to a different extent. The profile of apoLp-III forms in the hemolymph, hemocytes, and fat body of the exoA-challenged larvae was analyzed using two-dimensional electrophoresis (IEF/SDS-PAGE) and immunoblotting with anti-apoLp-III antibodies. Two apoLp-III forms differing in isoelectric point values estimated at ∼6.5 and ∼6.1 in the hemolymph and ∼6.5 and ∼5.9 in the hemocytes as well as one isoform with pI ∼ 6.5 in the fat body with an additional apoLp-III-derived polypeptide with estimated pI∼6.9 were detected in the control insects. The injection of exoA caused a significant decrease in the abundance of both apoLp-III isoforms in the insect hemolymph. In the hemocytes, a decrease in the amount of the pI∼5.9 isoform was detected, while the major apoLp-III isoform (pI∼6.5) remained unchanged. In addition, appearance of an additional apoLp-III-derived polypeptide with an estimated pI∼5.2 was observed. Interestingly, there were no statistically significant differences in the amount of the main isoform in the fat body between the control and exoA-challenged insects, but the polypeptide with pI ∼6.9 disappeared completely. It should be noted that the decrease in the amount of apoLp-III and other proteins was especially noticeable at the time points when exoA was detected in the studied tissues.","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41370554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet impacts the reproductive system’s maturation in the male moth Agrotis ipsilon (Noctuidae, Lepidoptera)","authors":"Evan Force ,&nbsp;Philippe Couzi ,&nbsp;Matthieu Dacher ,&nbsp;Stéphane Debernard","doi":"10.1016/j.jinsphys.2023.104532","DOIUrl":"10.1016/j.jinsphys.2023.104532","url":null,"abstract":"<div><p>In male moth <em>Agrotis ipsilon</em>, sexual maturation occurs between the third and the fifth day of adult life and is characterized by the development of the reproductive organs such as testes and accessory sex glands. Since sexual maturation requires considerable energy investment, we hypothesized that diet would be an essential regulatory factor in this developmental process. Indeed, the links between the male diet and reproductive physiology have not been described as in females. To test the previous hypothesis, we offered male moths diets corresponding to different flower nectars found in nature, and measured morphological and functional changes in the testes and accessory sex glands. In comparison to a diet composed of sucrose only, males fed with a diet composed of diverse sugars, including glucose, supplemented with sodium led to an earlier increase in the length and the protein content of accessory sex glands, as well as a reduction of the testicular volume accompanied by an acceleration of the sperm bundle transfer from the testes to the duplex. These results show that these specific diets accelerate the maturation of the reproductive system in male moth <em>Agrotis ipsilon</em>.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9800247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature and age, individually and interactively, shape the size, weight, and body composition of adult female mosquitoes","authors":"Jordyn S. Barr,&nbsp;Tania Y. Estevez-Lao,&nbsp;Marina Khalif,&nbsp;Saksham Saksena,&nbsp;Sagnik Yarlagadda,&nbsp;Ommay Farah,&nbsp;Yasmine Shivere,&nbsp;Julián F. Hillyer","doi":"10.1016/j.jinsphys.2023.104525","DOIUrl":"10.1016/j.jinsphys.2023.104525","url":null,"abstract":"<div><p>Most insects are poikilotherms and ectotherms, so their body temperature fluctuates and closely aligns with the temperature of their environment. The rise in global temperatures is affecting the physiology of insects by altering their ability to survive, reproduce, and transmit disease. Aging also impacts insect physiology because the body deteriorates via senescence as the insect ages. Although temperature and age both impact insect biology, these factors have historically been studied in isolation. So, it is unknown whether or how temperature and age interact to shape insect physiology. Here, we investigated the effects of warmer temperature (27 °C, 30 °C and 32 °C), aging (1, 5, 10, and 15 days post-eclosion), and their interaction on the size and body composition of the mosquito, <em>Anopheles gambiae</em>. We found that warmer temperatures result in slightly smaller adult mosquitoes, as measured by abdomen and tibia length. Aging alters both abdominal length and dry weight in a manner that correlates with the increase in energetic resources and tissue remodeling that occurs after metamorphosis and the senescence-based decline that ensues later. Moreover, the carbohydrate and lipid contents of adult mosquitoes are not meaningfully affected by temperature but are altered by aging: carbohydrate content increases with age whereas lipid content increases over the first few days of adulthood and then decreases. Protein content decreases with both rising temperature and aging, and the aging-associated decrease accelerates at warmer temperatures. Altogether, temperature and age, individually and to a lesser extent interactively, shape the size and composition of adult mosquitoes.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10152845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calcium storage in Malpighian tubules and the putative use for pupal chamber formation in a wood-feeding insect","authors":"Yuichi Yamamoto ,&nbsp;Yuko Fujiwara","doi":"10.1016/j.jinsphys.2023.104534","DOIUrl":"10.1016/j.jinsphys.2023.104534","url":null,"abstract":"<div><p>Cerambycid beetles form a chamber to spend their pupal stages in various forms according to the species. The red-necked longhorn beetle <em>Aromia bungii</em> (Coleoptera: Cerambycidae), which is an invasive pest that severely damages Rosaceae trees, makes a pupal chamber at the end of a tunnel deep in the xylem. Beetle larvae and the closely related species form a calcareous lid at the entrance of a pupal chamber. Previous studies on the closely related species conducted more than a century ago suggested that Malpighian tubules (MTs) play a vital role in calcium carbonate accumulation. However, the association between this Ca²⁺ accumulation and pupal chamber lid formation utilizing the possible calcium compounds stored in MTs have not yet been demonstrated. First, we artificially reared <em>A. bungii</em> larvae from eggs in host branches for 100 days and identified the larval developmental status and pupal chamber formation, using X-ray computed tomography. Second, we collected larvae from the branches and observed the internal organs by direct dissection under a microscope. Finally, we analyzed the elemental distribution, particularly calcium, in the larval gut with MTs, using energy dispersive X-ray fluorescence. The results suggest that immature larvae of <em>A. bungii</em> can accumulate Ca²⁺ in the MTs through wood tunneling and feeding activities. Ca²⁺ was stored at the proximal regions in two of the six MTs located posteriorly in the body. Additionally, larvae that formed a calcareous lid at the entrance of pupal chambers in the branches did not store Ca²⁺ in the MTs, suggesting that the larvae of <em>A. bungii</em> used the stored Ca²⁺ in their MTs for lid formation.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9790676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diet quality influences nutrient retention and reproductive fitness of the biocontrol agent Hadronotus pennsylvanicus (Hymenoptera: Scelionidae)","authors":"Robert K. Straser,&nbsp;Joshua E. Reger,&nbsp;Houston Wilson","doi":"10.1016/j.jinsphys.2023.104522","DOIUrl":"10.1016/j.jinsphys.2023.104522","url":null,"abstract":"<div><p><span>Diet can have a direct influence on the reproductive success<span><span> of parasitoid<span> wasps. For synovigenic parasitoids, the nutrients obtained from floral resources, such as nectar and pollen, play a vital role in fueling bodily functions and physiological energy expenditure incurred from reproduction. Insufficient access to nutrient-rich diets can lead to lower rates of </span></span>reproductive fitness, therefore reducing the efficacy of biocontrol. Here, a study was conducted to evaluate the influence of diet quality on nutrient retention and reproductive fitness of the egg parasitoid </span></span><em>Hadronotus pennsylvanicus</em> (Hymenoptera: Scelionidae), a prospective biocontrol agent for the leaffooted bug <em>Leptoglossus zonatus</em><span><span> (Heteroptera: Coreidae), a primary pest of almonds and pistachios. Newly emerged parasitoid females were provided host eggs every other day accompanied by diets of varying sucrose concentrations (source of carbohydrates) and pollen (source of </span>lipid and proteins). The sucrose concentration in the diet, regardless of pollen content, significantly increased the survival and lifetime fecundity of female </span><em>H. pennsylvanicus</em>. While wasps fed high sucrose diets depleted bodily sugars, glycogen, and lipids at a slower rate than wasps fed low sucrose diets, there was no effect on bodily protein levels. Given these findings, further research is now needed to identify floral resources that are compatible, attractive, and nutritionally-sufficient for optimal <em>H. pennsylvanicus</em> reproductive fitness, which could lead to enhanced parasitism of <em>L. zonatus</em> in crop systems.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9679260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute cold stress and supercooling capacity of Mediterranean fruit fly populations across the Northern Hemisphere (Middle East and Europe)","authors":"Georgia D. Papadogiorgou ,&nbsp;Cleopatra A. Moraiti ,&nbsp;David Nestel ,&nbsp;John S. Terblanche ,&nbsp;Eleni Verykouki ,&nbsp;Nikos T. Papadopoulos","doi":"10.1016/j.jinsphys.2023.104519","DOIUrl":"10.1016/j.jinsphys.2023.104519","url":null,"abstract":"<div><p>The Mediterranean fruit fly, <em>Ceratitis capitata</em> (Diptera: Tephritidae), holds an impressive record of successful invasion events promoted by globalization in fruit trade and human mobility. In addition, <em>C. capitata</em> is gradually expanding its geographic distribution to cooler temperate areas of the Northern Hemisphere. Cold tolerance of <em>C. capitata</em> seems to be a crucial feature that promotes population establishment and hence invasion success. To elucidate the interplay between the invasion process in the northern hemisphere and cold tolerance of geographically isolated populations of <em>C. capitata</em>, we determined (a) the response to acute cold stress survival of adults, and (b) the supercooling capacity (SCP) of immature stages and adults. To assess the phenotypic plasticity in these populations, the effect of acclimation to low temperatures on acute cold stress survival in adults was also examined. The results revealed that survival after acute cold stress was positively related to low temperature acclimation, except for females originating from Thessaloniki (northern Greece). Adults from the warmer environment of South Arava (Israel) were less tolerant after acute cold stress compared with those from Heraklion (Crete, Greece) and Thessaloniki. Plastic responses to cold acclimation were population specific, with the South Arava population being more plastic compared to the two Greek populations. For SCP, the results revealed that there is little to no correlation between SCP and climate variables of the areas where <em>C. capitata</em> populations originated. SCP was much lower than the lowest temperature individuals are likely to experience in their respective habitats. These results set the stage for asking questions regarding the evolutionary adaptive processes that facilitate range expansions of <em>C. capitata</em> into cooler temperate areas of Europe.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9678823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of Gram-positive and Gram-negative bacterial toxins (LTA & LPS) on cardiac function in Drosophila melanogaster larvae","authors":"Kaitlyn E. Brock,&nbsp;Elizabeth R. Elliott,&nbsp;Maya O. Abul-Khoudoud,&nbsp;Robin L. Cooper","doi":"10.1016/j.jinsphys.2023.104518","DOIUrl":"10.1016/j.jinsphys.2023.104518","url":null,"abstract":"<div><p>The effects of Gram negative and positive bacterial sepsis depend on the type of toxins released, such as lipopolysaccharides (LPS) or lipoteichoic acid (LTA). Previous studies show LPS to rapidly hyperpolarize larval <em>Drosophila</em> skeletal muscle, followed by desensitization and return to baseline. In larvae, heart rate increased then decreased with exposure to LPS. However, responses to LTA, as well as the combination of LTA and LPS, on the larval <em>Drosophila</em> heart have not been previously examined. This study examined the effects of LTA and a cocktail of LTA and LPS on heart rate. The combined effects were examined by first treating with either LTA or LPS only, and then with the cocktail. The results showed a rapid increase in heart rate upon LTA application, followed by a gradual decline over time. When applying LTA followed by the cocktail, an increase in the rate occurred. However, if LPS was applied before the cocktail, the rate continued declining. These responses indicate the receptors or cellular cascades responsible for controlling heart rate within seconds and the rapid desensitization are affected by LTA or LPS and a combination of the two. The mechanisms for rapid changes which are not regulated by gene expression by exposure to LTA or LPS or associated bacterial peptidoglycans have yet to be identified in cardiac tissues of any organism.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9675744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aphidius ervi venom regulates Buchnera contribution to host nutritional suitability","authors":"Elia Russo ,&nbsp;Ilaria Di Lelio ,&nbsp;Min Shi ,&nbsp;Andrea Becchimanzi ,&nbsp;Francesco Pennacchio","doi":"10.1016/j.jinsphys.2023.104506","DOIUrl":"10.1016/j.jinsphys.2023.104506","url":null,"abstract":"<div><p>The association between the pea aphid, <em>Acyrthosiphon pisum</em> (Harris) (Homoptera: Aphididae), and the endophagous parasitoid wasp <em>Aphidius ervi</em> Haliday (Hymenoptera: Braconidae) offers a unique model system for studying the molecular mechanisms underlying the complex interactions between the parasitoid, its host and the associated primary symbiont. Here, we investigate <em>in vivo</em> the functional role of the most abundant component of <em>A. ervi</em> venom, <em>Ae-</em>γ-glutamyl transpeptidase (<em>Ae-</em>γ-GT), which is known to induce host castration. Microinjections of double-stranded RNA into <em>A. ervi</em> pupae stably knocked down <em>Ae-γ-GT1</em> and <em>Ae-γ-GT2</em> paralogue genes in newly emerged females. These females were used to score the phenotypic changes both in parasitized hosts and in the parasitoid’s progeny, as affected by a venom blend lacking <em>Ae</em>-γ-GT. <em>Ae-γ-GT</em> gene silencing enhanced growth both of host and parasitoid, supported by a higher load of the primary bacterial symbiont <em>Buchnera aphidicola.</em> Emerging adults showed a reduced survival and fecundity, suggesting a trade-off with body size. This demonstrates <em>in vivo</em> the primary role of <em>Ae</em>-γ-GT in host ovary degeneration and suggests that this protein counterbalances the proliferation of <em>Buchnera</em> likely triggered by other venom components<em>.</em> Our study provides a new approach to unravelling the complexity of aphid parasitoid venom <em>in vivo,</em> and sheds light on a novel role for <em>Ae</em>-γ-GT in host regulation.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9678781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tropical super flies: Integrating Cas9 into Drosophila ananassae and its phenotypic effects","authors":"Vera M. Yılmaz ,&nbsp;Timothy J.S. Ramnarine ,&nbsp;Annabella Königer ,&nbsp;Selina Mussgnug ,&nbsp;Sonja Grath","doi":"10.1016/j.jinsphys.2023.104516","DOIUrl":"10.1016/j.jinsphys.2023.104516","url":null,"abstract":"<div><p>Ectotherms such as insects are animals whose body temperature largely depends on ambient temperature and temperature variations provide a selection pressure affecting the geographical distribution of these species. However, over the course of evolution, some insect species managed to colonize environments characterized by various temperature ranges. Therefore, insects provide an excellent study system to investigate the basis of adaptation to temperature changes and extremes. We are generally using the vinegar fly <em>Drosophila ananassae</em> as a model system to investigate the genetic basis of cold tolerance. This species has expanded from its tropical ancestral range to more temperate regions resulting in a cosmopolitan, domestic distribution. Previously, we identified candidate genes significantly associated with cold tolerance in this species. We now established molecular genetic tools to assess the function of these genes. Using CRISPR/<em>Cas9</em> methodology for genome editing and the PiggyBac system, the <em>Cas9</em> enzyme was successfully integrated into the genome of three fly strains with different levels of cold tolerance. We further report on preliminary findings that the <em>Cas9</em> integration itself did not have a consistent effect on tolerance to cold. In conclusion, we offer with our study the molecular tools that allow studying stress-related candidate genes in <em>D. ananassae</em> in the future. In addition, we point out and provide guidance on the challenges that come with genome editing in a non-model species.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10049067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"No physiological costs of dual sequestration of chemically different plant toxins in the milkweed bug Spilostethus saxatilis (Heteroptera: Lygaeidae)","authors":"Laura Espinosa del Alba,&nbsp;Georg Petschenka","doi":"10.1016/j.jinsphys.2023.104508","DOIUrl":"10.1016/j.jinsphys.2023.104508","url":null,"abstract":"<div><p>Many herbivorous insects not only cope with plant toxins but also sequester them as a defense against predators and parasitoids. Sequestration is a product of the evolutionary arms race between plants and herbivorous insects and has been hypothesized to incur physiological costs due to specific adaptations required. Contradictory evidence about these costs exists for insects sequestering only one class of toxin, but very little is known about the physiological implications for species sequestering structurally different classes of compounds. <em>Spilostethus saxatilis</em> is a milkweed bug belonging to the cardenolide-sequestering heteropteran subfamily Lygaeinae (Heteroptera: Lygaeidae) that has shifted to the colchicine-containing plant <em>Colchicum autumnale,</em> a resource of chemically unrelated alkaloids. Using feeding-assays on artificial diet and chemical analysis, we assessed whether <em>S. saxatilis</em> is still able to sequester cardenolides apart from colchicine and related metabolites (colchicoids), and tested the effect of (1) either a natural cardenolide concentration (using ouabain as a model compound) or a natural colchicine concentration, (2) an increased concentration of both toxins, and (3) seeds of either <em>Asclepias syriaca</em> (cardenolides) or <em>C. autumnale</em> (colchicoids) on a set of life-history traits. For comparison, we assessed the same life-history traits in the milkweed bug <em>Oncopeltus fasciatus</em> exposed to cardenolides only. Although cardenolides and colchicoids have different physiological targets (Na⁺/K⁺-ATPase vs tubulin) and thus require different resistance traits, chronic exposure and sequestration of both isolated toxins caused no physiological costs such as reduced growth, increased mortality, lower fertility, or shorter adult life span in <em>S. saxatilis</em>. Indeed, an increased performance was observed in <em>O. fasciatus</em> and an according trend was found in <em>S. saxatilis</em> when feeding on isolated ouabain and isolated colchicine, respectively. Positive effects were even more pronounced when insects were provided with natural toxic seeds (i.e. <em>C. autumnale</em> for <em>S. saxatilis</em> and <em>A. syriaca</em> for <em>O. fasciatus</em>), especially in <em>O. fasciatus</em>. Our findings suggest, that <em>S. saxatilis</em> can sequester two chemically unrelated classes of plant compounds at a cost-free level, and that colchicoids may even play a beneficial role in terms of fertility.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9675705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}