Journal of insect physiology最新文献

{"title":"Effects of temperature and humidity on the survival and hatching response of diapausing and non-diapausing Aedes aegypti eggs","authors":"Sylvia Fischer ,&nbsp;María Sol De Majo ,&nbsp;Cristian Di Battista ,&nbsp;Raúl E. Campos","doi":"10.1016/j.jinsphys.2024.104726","DOIUrl":"10.1016/j.jinsphys.2024.104726","url":null,"abstract":"<div><div>In seasonally varying environments, diapause, which is induced by a short photoperiod, favors overwintering of many insects. In Aedine mosquitoes, embryonic diapause is associated with higher survival and resistance to low temperature and humidity. <em>Aedes aegypti</em>, the main vector of dengue and other arboviruses, has recently expanded its distribution towards temperate regions. One of the mechanisms that might have favored this expansion in South America is the ability to induce embryonic diapause. This type of diapause has been recently discovered in populations from Argentina, associated with hatching inhibition and increased amounts of lipids in the eggs. The aim of this study was to assess the four-month survival of diapausing (D) and non-diapausing (ND) eggs stored at different humidity and temperature conditions. Two populations from the temperate region of Argentina were analyzed: one from Buenos Aires (BA), a city with a relatively mild and short winter, and another from San Bernardo (SB), a locality with a harsher and longer winter. For both populations, D and ND eggs were obtained from colonies maintained under 10:14 L:D and 14:10 L:D hours respectively. Eggs were exposed to six different conditions of humidity and temperature for 85 days. After exposure, egg survival and hatching response were analyzed. D eggs showed significantly higher survival at low humidity (both populations), and at medium and high humidity and at low temperatures (SB population). In addition, D eggs showed a significantly lower hatching response at high humidity and low temperatures, and higher proportion of not hatched eggs remaining viable after two immersions under all conditions. D eggs from SB were significantly more tolerant to low temperatures than those from BA. ND eggs from SB were significantly more tolerant to low temperatures, while those from BA were more tolerant to low humidity. Overall, the effect of diapause was a significant increase in the number of not hatched, viable embryos after immersion. Results suggest that the ability of <em>Ae. aegypti</em> to induce egg diapause increases the probability of successful overwintering and further expansion of its distribution range, and as a consequence the risk of arbovirus transmission might increase in temperate areas.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104726"},"PeriodicalIF":2.3,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142785356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Cellular dynamics of host − parasitoid interactions: Insights from the encapsulation process in a partially resistant host” [J. Insect Physiol. 155 (2024) 104646]","authors":"Samuel Gornard,&nbsp;Florence Mougel,&nbsp;Isabelle Germon,&nbsp;Véronique Borday-Birraux,&nbsp;Pascaline Venon,&nbsp;Salimata Drabo,&nbsp;Laure Kaiser","doi":"10.1016/j.jinsphys.2024.104711","DOIUrl":"10.1016/j.jinsphys.2024.104711","url":null,"abstract":"","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104711"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essential role of eclosion hormone precursor and receptor genes in desert locust ecdysis","authors":"Lina Verbakel ,&nbsp;Cynthia Lenaerts ,&nbsp;Jerom Vranken ,&nbsp;Elisabeth Marchal ,&nbsp;Jozef Vanden Broeck","doi":"10.1016/j.jinsphys.2024.104736","DOIUrl":"10.1016/j.jinsphys.2024.104736","url":null,"abstract":"<div><div>The process of molting represents a critical phase in the life cycle of arthropods, marking periods of growth and development. Central to this process is the eclosion hormone (EH), a neurohormone that plays a pivotal role in initiating and regulating the complex sequence of events leading to successful molting in holometabolan species. Very little information is available in Hemimetabola, which display a different kind of development characterized by gradual changes. This paper reports on the identification of the two EH precursors and the EH receptor (EHR), a guanylyl cyclase, in a hemimetabolan pest species, the desert locust, <em>Schistocerca gregaria</em>. Using qRT-PCR, an in-depth profiling study of <em>Schgr</em>-EH-1, −2 and <em>Schgr</em>-EHR transcripts was performed. Silencing of <em>Schgr</em>-EH-1, −2 and <em>Schgr</em>-EHR resulted in lethality at the expected time of ecdysis, thereby showing their crucial role during this process.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104736"},"PeriodicalIF":2.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A male-killing Spiroplasma endosymbiont has age-mediated impacts on Drosophila endurance and sleep","authors":"Collin J. Horn ,&nbsp;Sissi Yuli ,&nbsp;Jacob A. Berry ,&nbsp;Lien T. Luong","doi":"10.1016/j.jinsphys.2024.104723","DOIUrl":"10.1016/j.jinsphys.2024.104723","url":null,"abstract":"<div><div>Endosymbiotic bacteria have a wide range of impacts on host physiology, behavior, metabolism, endurance, and mobility. Recent work found some endosymbionts also impact host sleep duration and quality. These effects may increase as flies age and endosymbiont titers increase. We tested the hypothesis that <em>Spiroplasma poulsonni</em> MSRO negatively impacts sleep in <em>Drosophila melanogaster,</em> and this in turn impairs fly endurance. In geotaxis climbing assays (a proxy for endurance), we found that MSRO impacted climbing endurance but in an age-dependent manner. Among younger flies, MSRO+ flies slept significantly less during dark periods (measured by a <em>Drosophila</em> Activity Monitoring System) compared to uninfected flies, but older MSRO+ flies did not show significant differences in amount of sleep compared to uninfected flies in the same cohort. While MSRO status impacted both sleep and endurance of hosts, endosymbiont-mediated sleep deprivation did not directly explain decreases in fly endurance. We discuss these results in the context of endosymbiont comparative biology.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104723"},"PeriodicalIF":2.3,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-34 disrupts border cell migration by targeting Eip74EF in Drosophila melanogaster","authors":"Jingya Xia,&nbsp;Lina Wang,&nbsp;Fengyun Lei,&nbsp;Lu Pan,&nbsp;Lijun Liu,&nbsp;Ping Wan","doi":"10.1016/j.jinsphys.2024.104724","DOIUrl":"10.1016/j.jinsphys.2024.104724","url":null,"abstract":"","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104724"},"PeriodicalIF":2.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light pollution disrupts seasonal reproductive phenotypes and reduces lifespan in the West Nile vector, Culex pipiens","authors":"Maria A. Fiorta ,&nbsp;Lydia R. Fyie ,&nbsp;Megan E. Meuti","doi":"10.1016/j.jinsphys.2024.104725","DOIUrl":"10.1016/j.jinsphys.2024.104725","url":null,"abstract":"<div><div>Females of the Northern House mosquito, <em>Culex pipiens</em>, are important disease vectors as they transmit pathogens including West Nile virus. These females survive the winter by entering diapause, a state of dormancy, characterized by the accumulation of lipids, cessation of blood-feeding, and reproductive arrest. Diapause is cued by photoperiod, so as days become short in late summer and early fall, female <em>Cx. pipiens</em> prepare to overwinter and disease transmission decreases. We previously demonstrated that Artificial light at night (ALAN) causes female <em>Cx. pipiens</em> to avert diapause and continue to blood-feed when reared under short-day conditions. Additionally, light pollution alters seasonal differences in mosquito activity and nutrient reserves. However, it is unclear how exposure to ALAN affects blood-feeding and fecundity in long-day reared females, as well as the survival of <em>Cx. pipiens</em> exposed under both short and long-day conditions. In this study, we hypothesized that females exposed to ALAN in long-day conditions would have a lower proclivity to blood-feed, reduced fecundity, and reduced survival. Results from our lab-based experiments demonstrate that females exposed to ALAN in long-day conditions were less likely to blood-feed but were more fecund than long-day reared females that were not exposed to ALAN, and that ALAN exposure did not affect lifespan of long-day reared females. Additionally, we hypothesized ALAN exposure under short-day conditions would reduce survival, and our data supports this hypothesis. Overall, our results demonstrate that ALAN is an important urban stressor that has the potential to affect reproduction and lifespan in mosquitoes, and therefore has the potential to create evolutionary tradeoffs.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104725"},"PeriodicalIF":2.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gonadal development of adultoid reproductive in subterranean termites indicates strong reproductive potential","authors":"Jia Wu ,&nbsp;Xiaolan Wen ,&nbsp;Jinpei Wang ,&nbsp;Zhiyong Yue ,&nbsp;Qi Jiang","doi":"10.1016/j.jinsphys.2024.104721","DOIUrl":"10.1016/j.jinsphys.2024.104721","url":null,"abstract":"<div><div>The fair allocation of reproductive resources between population growth and outward dispersal plays a crucial role in determining competitive advantage and ecological success of organisms within their environment. Termite colonies demonstrate flexibility in allocating their reproductive resources through wing polymorphism: with alates possessing fully developed wings for dispersal to propagate the colony’s genetic material, while adultoids have floppy wings, serving a secondary reproductive to ensure stability and development within the natal colony. However, the extent of reproductive potential exhibited by alates (post-shed-wings called dealate) and adultoids within the colony remains uncertain. In this study, through experimentation, we compared the reproductive capacity of dealate and adultoid. We found that the adultoids can develop to maturity and lay eggs faster after emergence. The reproductive potential including the number of ovariole, the number of oocytes in growth and vitellogenesis stage, and the expression levels of vitellogenin genes were all higher than dealates. More importantly, paired adultoids has a longer oviposition cycle and is able to obtain more eggs and larvae. These results suggest that adultoids possess a greater reproductive potential allowing them to produce a large number of offspring within a short period of time. Our results help to an explanation of the biological significance that the differentiation of adultoids reproductive persist in lower termite colonies.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104721"},"PeriodicalIF":2.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antennal olfactory responses in the black soldier fly Hermetia illucens","authors":"Silvana Piersanti ,&nbsp;Manuela Rebora ,&nbsp;Giorgia Carboni Marri ,&nbsp;Gianandrea Salerno","doi":"10.1016/j.jinsphys.2024.104722","DOIUrl":"10.1016/j.jinsphys.2024.104722","url":null,"abstract":"<div><div>The Black Soldier Fly (BSF) is considered as the “crown jewel” of the insect feed industry and circular economy, significantly contributing to the 2030 Sustainable Development Goals by reducing carbon dioxide emissions and enabling circular management of organic waste, animal manure, and plant residues. Despite their industrial importance, limited knowledge about adult BSF biology has hindered optimal mass production. In this context, the present paper aims to explore the olfactory capabilities of both male and female BSF in response to various odorants commonly associated with organic decomposition in substrates suitable for mate encounters and egg laying. This will be achieved by performing electroantennographic recordings and scanning electron microscopy (SEM) observations on the antennal sensilla. Our results demonstrate for the first time the supposed olfactory capabilities of BSF antennae and present a first dataset of substances emitted by decaying organic matter detected by both male and female flies. Additionally, the current EAG recordings allowed comparisons with molecular data previously obtained through <em>in silico</em> and <em>in vitro</em> methods, highlighting the need for caution and strongly supporting a multidisciplinary approach as the best tool for investigating insect chemical ecology. These findings advance our understanding of BSF chemical ecology, which is crucial for effective reproduction and could significantly optimize global breeding systems.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104722"},"PeriodicalIF":2.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological trade-offs in male social insects: Interactions among infection, immunity, fertility, size, and age in honey bee drones","authors":"Bradley N. Metz ,&nbsp;Tatiana Molina-Marciales ,&nbsp;Micheline K. Strand ,&nbsp;Olav Rueppell ,&nbsp;David R. Tarpy ,&nbsp;Esmaeil Amiri","doi":"10.1016/j.jinsphys.2024.104720","DOIUrl":"10.1016/j.jinsphys.2024.104720","url":null,"abstract":"<div><div>Female social insects represent a dramatic exception of the evolutionarily conserved physiological trade-off between reproduction and life span, where aging is positively correlated with reproduction. However, whether this facet of life history also pertains to male social insects, remains largely unknown. Male honey bees (drones) die in the act of copulation, placing them under opposing selective pressures. At the individual level, there is inter-male competition for a single successful mating attempt, leading to selective pressure that favors an increase in male fitness. Honey bee drones are haploid individuals and lack the allelic variation in their genome compared to diploid females. We hypothesized that this genetic limitation may result in trade-offs between pathological stress and fitness traits in honey bee males. In our study, we observed differences in size and fertility measures in old and young drones along with stressors of several endemic viruses and the transcriptional immune response. We found that infection does not appear to decrease fertility in old drones, despite evidence for a shift in immune expression away from established mechanisms. Contrary to our expectations, drones additionally do not appear to exhibit a physiological trade-off between size and fertility. These findings demonstrate that drones of different size are likely of different mating quality and that higher quality drones likely favor retaining reproductive output over immune function.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104720"},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142605146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Central role of squid gene during oocyte development in the Hemiptera Rhodnius prolixus","authors":"Agustina Pascual ,&nbsp;Catalina Taibo ,&nbsp;Rolando Rivera-Pomar","doi":"10.1016/j.jinsphys.2024.104719","DOIUrl":"10.1016/j.jinsphys.2024.104719","url":null,"abstract":"<div><div>Oocyte polarity establishment is a conserved and crucial phenomenon for embryonic development. It relies on the precise spatial localization of maternal factors deposited during oocyte development, which is essential for establishing and maintaining cell polarity and subsequently specifying embryonic axes. The heterogeneous nuclear ribonucleoprotein (hnRNP) encoded by the <em>squid</em> (<em>sqd</em>) gene has been implicated in mRNA localization and embryonic axis establishment in <em>Drosophila melanogaster</em>. Comparative genomics allowed for the identification of a homologue in <em>Rhodnius prolixus</em>. In this study, we investigated the function of <em>Rp-sqd</em> during oogenesis and early embryonic development. We observed persistent expression of <em>Rp-sqd</em> during oocyte development, with localization in the cytoplasm of ovary germarium and growing oocytes in previtellogenic and vitellogenic stages. A Parental RNA interference (RNAi) experiment targeting <em>Rp-sqd</em> resulted in female sterility. The ovaries showed disrupted oocyte development, disarray of follicular epithelium, and affected nurse cells integrity. Immunostaining and microscopic techniques revealed microtubule disarray and a reduction in the presence of organelles in the trophic cords that connect the germarium with the oocytes. The <em>Rp-sqd</em> depletion impacted the transcript expression of maternal mRNAs involved in apoptosis, axis formation, oogenesis, and cytoskeleton maintenance, indicating a pleiotropic function of <em>Rp-sqd</em> during oogenesis. This study provides new insights into the genetic basis of <em>R. prolixus</em> oogenesis, highlighting the crucial role of <em>Rp-sqd</em> in oocyte development, fertility, and germarium integrity. These findings contribute to our understanding of insect developmental processes, provide a foundation for future investigations into reproduction, and reveal the regulatory mechanisms governing the process.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"159 ","pages":"Article 104719"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}