Long-term impacts of egg quiescence and Wolbachia infection on lipid profiles in Aedes aegypti: Ovarian roles in lipid synthesis during reproduction

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
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Abstract

Wolbachia, an endosymbiotic bacterium, relies on nutrients from its host to complete its life cycle. The presence of Wolbachia strain wAlbB in the mosquito Aedes aegypti during egg or larval stages affects the host’s development, leading to the absence of developed and visible ovaries in adult mosquito females. In this study, we investigated the impacts of egg quiescence and Wolbachia infection on lipid profiles of adult Ae. aegypti females, and discerned the role of ovaries in lipid synthesis in the reproductive process. The lipidomes of Wolbachia infected and uninfected female individuals at various developmental stages were quantitatively analyzed by LC-MS/MS. Lipidomic change patterns were systematically further investigated in wAlbB-infected fertile females and infertile females following blood feeding. Prolonged egg quiescence induced a shortage of acyl-carnitine (CAR) and potentially impacted some molecules of diacyl-phospholipid (diacyl-PL) and sphingolipid (SL) in young adult mosquitoes. After the first gonotrophic cycle, infertile females accumulated more CAR and lyso-phospholipid (lyso-PL) than fertile females. Then in the second gonotrophic cycle, the patterns of different lipid groups remained similar between fertile and infertile females. Only a small proportion of molecules of triglyceride (TG), phospholipid (lyso-PL and diacyl-PL) and ceramide (Cer) increased exclusively in fertile females from 0 h to 16 h post blood meal, suggesting that the generation or prescence of these lipids rely on ovaries. In addition, we found cardiolipins (CL) might be impacted by Wolbachia infection at the egg stage, and infected mosquitoes also showed distinct patterns between fertile and infertile females at their second gonotrophic cycle. Our study provides new insights into the long-term influence of Wolbachia on lipid profiles throughout various life stages of mosquitoes. Additionally, it suggests a role played by ovaries in lipid synthesis during mosquito reproduction.

Abstract Image

卵静止和沃尔巴克氏体感染对埃及伊蚊脂质特征的长期影响:卵巢在繁殖期间脂质合成中的作用。
沃尔巴克氏菌是一种内共生细菌,依靠宿主提供的营养来完成其生命周期。在埃及伊蚊的卵或幼虫阶段,沃尔巴克氏菌株 wAlbB 的存在会影响宿主的发育,导致成年雌蚊没有发育和可见的卵巢。在这项研究中,我们研究了卵静止和沃尔巴克氏体感染对成年埃及伊蚊雌虫脂质图谱的影响,并发现了卵巢在生殖过程中脂质合成的作用。采用 LC-MS/MS 对感染了沃尔巴克氏菌和未感染沃尔巴克氏菌的雌性个体在不同发育阶段的脂质体进行了定量分析。进一步系统地研究了经 wAlbB 感染的可育雌性个体和经血饲喂后的不育雌性个体的脂质体变化规律。长时间的卵静止诱导了酰基肉碱(CAR)的短缺,并可能影响年轻成蚊体内的一些二酰磷脂(diacyl-PL)和鞘脂(SL)分子。在第一个生殖周期后,未育雌蚊比已育雌蚊积累了更多的 CAR 和溶血磷脂(溶血磷脂)。然后,在第二个生殖周期中,可育雌蚊和不可育雌蚊的不同脂质组的模式仍然相似。只有一小部分甘油三酯(TG)、磷脂(溶菌酶-磷脂和二酰基-磷脂)和神经酰胺(Cer)分子在血餐后0小时至16小时内只在可育雌鼠体内增加,这表明这些脂质的产生或存在依赖于卵巢。此外,我们发现心磷脂(CL)可能会在卵阶段受到沃尔巴克氏体感染的影响,受感染的蚊子在其第二个生殖腺周期也表现出可育雌蚊和不育雌蚊的不同模式。我们的研究为了解沃尔巴克氏菌对蚊子各生命阶段脂质特征的长期影响提供了新的视角。此外,它还表明卵巢在蚊子繁殖过程中的脂质合成中发挥了作用。
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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