Journal of insect physiology最新文献

{"title":"Editorial 2025: Changes and the future of JIP","authors":"John S. Terblanche , H. Arthur Woods","doi":"10.1016/j.jinsphys.2025.104772","DOIUrl":"10.1016/j.jinsphys.2025.104772","url":null,"abstract":"","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104772"},"PeriodicalIF":2.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends and emerging hotspots in RNAi-based arthropod pest control: A comprehensive bibliometric analysis","authors":"Wenbin Liu ,&nbsp;Xinyu Wang ,&nbsp;Anmo Zhou ,&nbsp;Junyu Zhang ,&nbsp;Xinyu Ge ,&nbsp;Bernard Moussian ,&nbsp;Chuncai Yan ,&nbsp;Shaobo Gao ,&nbsp;Yiwen Wang","doi":"10.1016/j.jinsphys.2025.104754","DOIUrl":"10.1016/j.jinsphys.2025.104754","url":null,"abstract":"<div><div>RNA interference (RNAi)-based pest control has emerged as a cutting-edge and highly promising approach in pest control, especially for insect pests, due to its advantages of reduced environmental risk, degradability, and good selectivity. This study provides a bibliometric analysis of RNAi-based pest control, evaluating the global scientific output in this field from the Web of Science Core Collection (WoSCC) and PubMed. From 2007, when the first RNAi-based Arthropod pest control strategy suited for field application was published, to August 2024, 722 English research articles were identified, focusing only on dsRNA delivery modes including feeding, soaking, and spraying, which hold high potential for field application. Articles examining gene function and potential targets by dsRNA injection were excluded. The 722 eligible articles were published in 132 journals by 3112 authors from 563 institutions in fifty countries. Over these 17 years, the number of publications on RNAi-based pest control has shown a trend of accelerating growth. PEST MANAGEMENT SCIENCE published the most articles, followed by PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, SCIENTIFIC REPORTS. China produced most articles, followed by the United States. However, China is significantly behind the United States in developing commercial products in this field. Hot target insects in RNAi-based pest control research included <em>Bemisia tabaci</em>, <em>Helicoverpa armigera</em>, <em>Aphis gossypii</em> Glover, <em>Leptinotarsa decemlineata</em>, and <em>Diabrotica virgifera virgifera</em>. Frequently studied target genes included <em>vATPaseA</em>, <em>CHS1</em>, <em>SNF7</em>, <em>EcR</em> and <em>β-actin</em>, ect. In recent years, various advanced technologies for dsRNA delivery have been developed and utilized in RNAi-based pest control system, including nanoparticle-enabled, symbiont-mediated, and plant-mediated deliveries. This study represents the first comprehensive analysis based on bibliometric methods, aiming to investigate the forefront hotspots and research trends of RNAi-based pest control, providing valuable references for researchers and developers in this field.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104754"},"PeriodicalIF":2.3,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timing of the co-activation of antagonist muscles affects foreleg movements during mantis strikes","authors":"Sho Ogawa,&nbsp;Yoshifumi Yamawaki","doi":"10.1016/j.jinsphys.2025.104753","DOIUrl":"10.1016/j.jinsphys.2025.104753","url":null,"abstract":"<div><div>The foreleg movements during mantis strikes can be determined by three joint angles: the prothorax–coxa (P–C), coxa–trochanter (C–T), and femur–tibia (F–T). To understand how foreleg movements are controlled by muscle activities in the Chinese mantis, we combined motion analysis with electromyogram (EMG) recordings from three antagonist pairs, one at a time: the coxal promotor and remotor in the P–C joint, the trochanteral extensor and flexor in the C–T joint, and the tibial extensor and flexor in the F–T joint. During the P–C joint angle increase, a burst of spikes was observed in the EMG recordings from the coxal promotor. The promotor burst was accompanied by a burst of spikes in the coxal remotor. Co-activation of antagonist muscles, the extensor and flexor, was also observed at the C–T joint, but not at the F–T joint. The burst duration of the promotor or extensor mainly determined the peak angle of the P–C, C–T, and F–T joints. Co-activation of antagonists observed during P–C and C–T joint angle increases likely plays a role in adjusting the peak joint angle; the peak joint angle became smaller as remotor (or flexor) bursts occurred earlier. Variation in the timing of antagonist bursts may finely regulate the peak joint angle.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104753"},"PeriodicalIF":2.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plastic responses in sperm expenditure to sperm competition risk in black soldier fly (Hermetia illucens, Diptera) males","authors":"Frédéric Manas,&nbsp;Carole Labrousse,&nbsp;Christophe Bressac","doi":"10.1016/j.jinsphys.2025.104751","DOIUrl":"10.1016/j.jinsphys.2025.104751","url":null,"abstract":"<div><div>In polyandrous species, competition between males for offspring paternity goes on after copulation through the competition of their ejaculates for the fertilisation of the female’s oocytes. Given that males allocating more spermatozoa often have greater fertilisation success, different models of sperm competition predict adaptive plasticity in male sperm production and allocation. These predictions were tested experimentally in the black soldier fly (BSF) <em>Hermetia illucens</em>. In this farmed insect, adult biology is little known despite the economic interest of larvae for bioconversion, and as authorized feedstuff. In lab conditions, two sets of experiments were carried out to modify the risk of sperm competition perceived by males. The first consisted of placing adult males alone or in groups of 10 – modifying the mean risk of sperm competition – and then measuring their sperm production. The second took place at the beginning of copulation; pairs with males from the two treatments were transferred to different contexts of immediate risk of sperm competition: empty cages, cages containing 10 males, or cages containing 10 females. In all cases, spermatozoa transferred to mated females were counted. Males reared in groups of 10 showed more spermatozoa in their seminal vesicles than males reared alone. Regarding sperm allocation, females that mated in the presence of conspecifics – either 10 males or 10 females – received more spermatozoa than those that mated in isolation. In BSF, sperm production and allocation are influenced by the risk of sperm competition, demonstrating reproductive plasticity in response to socio-sexual conditions.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104751"},"PeriodicalIF":2.3,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acetylcholine and Malpighian tubules of the kissing bug, Rhodnius prolixus: Evidence for expression of the non-neuronal cholinergic system and a diuretic action of acetylcholine","authors":"Ian Orchard,&nbsp;Areej N. Al-Dailami,&nbsp;Angela B. Lange","doi":"10.1016/j.jinsphys.2025.104752","DOIUrl":"10.1016/j.jinsphys.2025.104752","url":null,"abstract":"<div><div>Acetylcholine (ACh) and its signaling pathways are expressed in peripheral tissues in mammals, leading to the concept of a non-neuronal cholinergic signaling system. This non-neuronal ACh is now known to participate as an autocrine/paracrine in many processes, including transport of ions and water, immunity, and reproduction. Non-neuronal cholinergic signaling in insects has only recently been explored, with transcriptome analyses disclosing the presence of transcripts for pivotal components of the cholinergic system in non-neuronal tissues, including Malpighian tubules (MTs), ovaries, hemocytes, and fat body. A recent transcriptome analysis of MTs of the Chagas disease vector <em>Rhodnius prolixus</em> revealed the presence of transcripts for muscarinic ACh receptors and for acetylcholinesterase, raising the possibility that non-neuronal ACh may play a role in the control of MTs. Here we provide evidence for a non-neuronal ACh signaling pathway in MTs of <em>R. prolixus</em>. Transcripts for ACh signaling are expressed, and ACh is capable of stimulating small increases in secretion by isolated MTs (small, relative to the true diuretic neurohormones in <em>R. prolixus</em>). The effects of ACh are mimicked by carbachol and pilocarpine, with atropine inhibiting ACh- and pilocarpine-stimulated secretion. Blood gorging alters expression of transcripts for ACh signaling. We discuss the physiological relevance for ACh since MTs are much more than diuresis; they play major roles in immunity, detoxification, and in tolerance to overall stress. Perhaps ACh is a signaling component of these essential functions that might require an increase in secretion rate by MTs that is separate from the well described rapid post-prandial diuresis.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104752"},"PeriodicalIF":2.3,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemocyanin contributes to embryonic adaptation to hypoxia in the migratory locust","authors":"Xianliang Huang ,&nbsp;Yuxing Xiao ,&nbsp;Yaru Yang ,&nbsp;Jiaqi Duan ,&nbsp;Deguang Liu","doi":"10.1016/j.jinsphys.2025.104750","DOIUrl":"10.1016/j.jinsphys.2025.104750","url":null,"abstract":"<div><div>Ambient hypoxia can pose a major threat to the survival of metazoan organisms, especially insect embryos. Hemocyanin exhibits dominant expression in insect embryos, but its specific roles in hypoxia adaptation remain unexplored. Soil-dwelling locust eggs may frequently experience hypoxia during development. A comprehensive analysis of physiological and biochemical characters of hemocyanin was conducted in the embryos of migratory locust <em>Locusta migratoria</em>. Our results demonstrated that the revolution process was the critical hypoxia-sensitive event during locust embryogenesis. Hemocyanin presented a prominent expression in the revolution stage and exhibited strong responses to hypoxia. The relative duration of revolution was correlated negatively with the expression of hemocyanin subunit 2 (HC2), suggesting that HC2 might be closely associated with hypoxia adaptation of locust embryos. Furthermore, a <em>HC2</em> mutant locust strain was established using the CRISPR/Cas9 technology, and higher hypoxia sensitivity was found for HC2-deficient locust embryos. Knockdown of <em>HC2</em> increased anaerobic metabolism and oxidative stress while reducing oxidative metabolism. Overall, these findings clearly demonstrated the pivotal roles of hemocyanin in hypoxia adaptation of insect embryos.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104750"},"PeriodicalIF":2.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful oral RNA interference efficiency in the silkworm Bombyx mori through nanoparticle-shielded dsRNA delivery","authors":"Jisheng Liu ,&nbsp;Yang Yang ,&nbsp;Qiangjun Yang ,&nbsp;Xianfeng Lin ,&nbsp;Yuming Liu ,&nbsp;Ziyang Li ,&nbsp;Luc Swevers","doi":"10.1016/j.jinsphys.2025.104749","DOIUrl":"10.1016/j.jinsphys.2025.104749","url":null,"abstract":"<div><div>Double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is a tool in functional gene study and pest control. However, RNAi efficiency in Lepidoptera is low compared to the RNAi sensitive Coleoptera. Previous studies on RNAi in the silkworm <em>Bombyx mori</em>, the lepidopteran model insect, were performed by injection only. Successful oral RNAi in the silkworm has never been reported yet. This study aims to develop a successful oral dsRNA delivery method to the silkworm larvae. Chitosan is an economical and biodegradable polymer. Chitosan/dsRNA nanoparticles were prepared by self-assembly. These nanoparticles were found to be stable when incubated in the midgut juice of the silkworm larvae, whereas naked dsRNA underwent complete degradation. Chitosan/dsRNA nanoparticles targeting various immune genes in oral administration to the silkworm larvae mediated significant knockdown of gene transcript. This silencing effect resulted in smaller larvae and cocoons when the silkworms were fed with Chitosan/dsRNA nanoparticles targeting <em>BmToll9-2</em> gene, indicating that immune genes might be used as targets in pest control. Optimization of the chitosan/dsRNA nanoparticles maintained an RNAi effect from 3-5 days. The efficient RNAi was due to the persistence of nanoparticle-shielded dsRNA in the larvae. The above findings contribute to the first oral RNAi report in the silkworm, which facilitates the application of RNAi in insects and oral RNAi in pest control.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104749"},"PeriodicalIF":2.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intestinal flow and digestive parameters of Lutzomyia longipalpis larvae","authors":"Luccas Gabriel Ferreira Malta ,&nbsp;Bianca Stacanelli Ribeiro ,&nbsp;Davi Viegas-Melo,&nbsp;Marques Serafim Pinho-Junior,&nbsp;Maurício Roberto Viana Sant’Anna,&nbsp;Marcos Horário Pereira,&nbsp;Nelder Figueiredo Gontijo","doi":"10.1016/j.jinsphys.2025.104748","DOIUrl":"10.1016/j.jinsphys.2025.104748","url":null,"abstract":"<div><div><em>Lutzomyia longipalpis</em> Lutz &amp; Neiva, 1912 (Diptera, Psychodidae), is the primary vector of <em>Leishmania infantum</em> Nicole, 1908, the etiological agent of American visceral leishmaniasis. During their development, sandfly larvae pass through four instars, consuming soil particles enriched with microorganisms and decomposing organic material. In numerous insect species, the intestinal epithelium not only secretes digestive enzymes and absorbs digested nutrients but also carries out additional functions, such as regulating luminal pH and facilitating the absorption or secretion of ions and water. The transport of ions and water plays a crucial role in the establishment of a countercurrent flow responsible for recycling soluble digestive enzymes. This study aimed to explore specific aspects of digestion <em>in L. longipalpis</em> larvae that remain poorly understood. We measured the intestinal flow within the endoperitrophic space, which varied depending on the type of diet offered to the larvae, with an average total time of 191 min. Additionally, we demonstrated the countercurrent flow in <em>L. long</em>ipalpis larvae. Finally, we showed that the production of digestive enzymes can be modulated by nutrient availability, particularly by the amino acids in the larval diet. The higher the amino acids concentration, the higher the trypsin activity. On the other hand, the aminopeptidase activity was poorly influenced by the amino acids concentration.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"161 ","pages":"Article 104748"},"PeriodicalIF":2.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving parasitism success of a weakly virulent parasitoid strain","authors":"Samuel Gornard,&nbsp;Pascaline Venon,&nbsp;Salimata Drabo,&nbsp;Laure Kaiser,&nbsp;Florence Mougel","doi":"10.1016/j.jinsphys.2024.104742","DOIUrl":"10.1016/j.jinsphys.2024.104742","url":null,"abstract":"<div><div>Endoparasitoids possess a whole set of virulence factors to counter the immune response of their host, among which can be found venom, endosymbiotic viruses and ovarian proteins. Depending on the species, some factors are expected to be less necessary than others. Notably, venom is reported as inessential in some parasitoids bearing viruses. We investigated the virulence factors of <em>Cotesia typhae</em>, a gregarious endoparasitoid of <em>Sesamia nonagrioides</em>, using highly and poorly virulent strains (CtV+ and CtV−). We tested if virulence of CtV− toward a reference strain could be improved by superparasitism (two females per host) or by injection of CtV+ virulence factors (venom and/or ovarian fluid). The parasitism success of CtV− could be increased by superparasitism with one female CtV+ confirming the importance of the virulence factors. Superparasitism with at least one CtV+ female decreased each female’s reproductive success and increased the number of non-viable offspring revealing larval competition. Parasitism order impacted the offspring proportion of each strain in the progeny, suggesting adaptation of egg-laying behavior in response to an already parasitized host. Injection of CtV+ venom or ovarian fluid alone maintained CtV− success unchanged, but their combination increased it to the one of CtV+. Altogether, this study hints towards larval competition, potentially specific to CtV+ strain, and suggests that <em>C. typhae</em> females can adapt their oviposition behavior based on the parasitic status of their host. It also demonstrates that, like several other <em>Cotesia</em> species, <em>C. typhae</em> resorts to a combination of venom and ovarian fluid to overcome the host’s immune responses.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"160 ","pages":"Article 104742"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A potential role for the interaction of Wolbachia surface proteins with the Drosophila microtubulin in maintenance of endosymbiosis and affecting spermiogenesis","authors":"Bin Mao ,&nbsp;Ying-Ying Wang ,&nbsp;Si-Ying Li ,&nbsp;Yue Fu ,&nbsp;Yun-Li Xiao ,&nbsp;Yu-Feng Wang","doi":"10.1016/j.jinsphys.2024.104743","DOIUrl":"10.1016/j.jinsphys.2024.104743","url":null,"abstract":"<div><div><em>Wolbachia</em>, as a widely infected intracellular symbiotic bacterium in Arthropoda, is able to manipulate the reproduction of insect hosts for facilitating their own transmission. Cytoplasmic incompatibility (CI) is the most common phenotype that <em>Wolbachia</em> induced in insect hosts where they resulted in the failure of uninfected egg hatch when fertilized with the sperm derived from <em>Wolbachia</em>-infected males, suggesting that the sperm are modified by <em>Wolbachia</em> during spermatogenesis. Although the molecular mechanisms of CI are beginning to be understood, the effects of <em>Wolbachia</em> on the symbiotic relationship and the proper dynamics of spermatogenesis have not yet been fully investigated. We report here that <em>Wolbachia</em> infection induced a significant upregulation of betaTub85D in the testis of <em>Drosophila melanogaster</em>. Knockdown of <em>betaTub85D</em> in fly testes resulted in significant decrease in the copy number of <em>Wolbachia</em> surface protein gene (<em>wsp</em>), indicating a notable reduction of <em>Wolbachia</em> density. Pull-down analyses revealed that WSP interacted with the betaTub85D of <em>D. melanogaster</em>. <em>Wolbachia</em> infection altered the interactome between betaTub85D and other proteins in the testes, and may thus change the protein synthesis and metabolic pathways. <em>Wolbachia</em> infection induced not only an interaction of betaTub85D with Mst77F but also increase in phosphorylated Mst77F. These results suggest that <em>Wolbachia</em> WSP protein might play important roles in anchoring the endosymbiont to the host’s cytoskeleton and consequently interfere the interactions among key proteins involved in spermatogenesis in the insect host testes, resulting in modified sperm.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"160 ","pages":"Article 104743"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}