Knockdown of LmSPARC influences lipid metabolic homeostasis involved ovarian development in Locusta migratoria

IF 2.3 2区农林科学 Q1 ENTOMOLOGY

Journal of insect physiology Pub Date : 2025-07-17 DOI:10.1016/j.jinsphys.2025.104855

Mingjun Wang , Wenhui Guo , Yong Yang , Qingyao Zhu , Xiaohong Zhang , Daochuan Zhang

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引用次数: 0

Abstract

Reproduction, a fundamental feature of all known life, is closely correlated with energy homeostasis. Lipids, which can accumulate, provide essential energy support for reproduction. Secreted Protein, Acidic and Rich in Cysteine (SPARC) is a cellular matrix glycoprotein involved in the regulation of extracellular matrix synthesis. However, the precise coordination of lipid homeostasis by SPARC during insect reproductive development remains poorly understood. Our study revealed that when the expression of LmSPARC was downregulated by RNA interference (RNAi) in 4th and 5th instar nymphs, molting was delayed compared with that in the control. Knockdown of LmSPARC reduced levels of vitellogenin (Vg) protein and retarded maturation of oocytes. The intercellular patency in the follicular epithelium of the ovaries was abnormal after LmSPARC silencing. In addition, transcriptomic and metabolomic analyses showed that the energy metabolism pathway was disrupted in locusts lacking LmSPARC, and the accumulation and degradation of lipids in the body were abnormal. This study provides insights into the effects of LmSPARC on ovarian development and energy metabolism homeostasis in Locusta migratoria, and provides a reference for screening biological control targets via on RNAi.

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LmSPARC基因敲低影响迁徙蝗卵巢发育过程中的脂质代谢稳态。

生殖是所有已知生命的基本特征，它与能量稳态密切相关。脂质可以积累，为生殖提供必要的能量支持。分泌蛋白，酸性和富含半胱氨酸（SPARC）是一种细胞基质糖蛋白，参与调节细胞外基质合成。然而，在昆虫生殖发育过程中，SPARC对脂质稳态的精确协调仍然知之甚少。我们的研究发现，当通过RNA干扰（RNAi）下调LmSPARC的表达时，4龄和5龄若虫的蜕皮时间比对照推迟。敲低LmSPARC可降低卵黄蛋白原（Vg）蛋白水平，延缓卵母细胞成熟。LmSPARC沉默后卵巢卵泡上皮细胞间通畅异常。此外，转录组学和代谢组学分析表明，缺乏LmSPARC的蝗虫能量代谢途径被破坏，体内脂质积累和降解异常。本研究揭示了LmSPARC对迁徙蝗卵巢发育和能量代谢稳态的影响，为通过RNAi筛选生物防治靶点提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of insect physiology 生物-昆虫学

CiteScore

4.50

自引率

4.50%

发文量

审稿时长

57 days

期刊介绍： All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.