Anam Noreen Abbas , Mubashir Tariq , Faisal Munir , Uroosa Zaheer , Ibrahim Adam , Muhammad Asad , Guang Yang
{"title":"Fox基因在小菜蛾发育和繁殖中的作用","authors":"Anam Noreen Abbas , Mubashir Tariq , Faisal Munir , Uroosa Zaheer , Ibrahim Adam , Muhammad Asad , Guang Yang","doi":"10.1016/j.jinsphys.2025.104868","DOIUrl":null,"url":null,"abstract":"<div><div>The forkhead box (Fox) family of transcription factors is essential for controlling physiological functions, metabolism and development in insects. However, the role of the Fox gene in the diamondback moth, <em>Plutella xylostella</em>, a major agricultural pest of cruciferous crops, is still unknown. The purpose of this work was to characterize the <em>PxFox1</em> gene and investigate its role in the reproduction and development of <em>P. xylostella</em>. The <em>Fox</em> gene in <em>P. xylostella (PxFox1</em>) is identified from the <em>P. xylostella</em> genome. The <em>Fox</em> gene was conserved in lepidoptera with the presence of Forkhead domain. <em>PxFox1</em> was expressed in different developmental stages and tissues of <em>P. xylostella</em>, highly in the female adult and integument. The <em>PxFox1</em> knockout mutants were generated by using the CRISPR/Cas9 technique. The significant reduction in the number of eggs and hatching rate was observed in mutants as compared with the wild-type. Meanwhile, an extended larval duration, shortened pupal phase, and reduced adult lifespan were observed in the mutants. Furthermore, <em>PxFox1</em> mutants displayed significant phenotypic abnormalities such as malformed wings, irregular body segmentation, and reduced body size, highlighting the gene’s essential role in the morphological development. These results showed that <em>PxFox1</em> is necessary for <em>P. xylostella</em> development, survival, and reproduction. Targeting <em>PxFox1</em> could provide a potential genetic pest control strategy for managing <em>P. xylostella</em> populations, offering new insights into insect gene regulation for sustainable pest management.</div></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":"165 ","pages":"Article 104868"},"PeriodicalIF":2.3000,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Roles of Fox gene in the development and reproduction of diamondback moth\",\"authors\":\"Anam Noreen Abbas , Mubashir Tariq , Faisal Munir , Uroosa Zaheer , Ibrahim Adam , Muhammad Asad , Guang Yang\",\"doi\":\"10.1016/j.jinsphys.2025.104868\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The forkhead box (Fox) family of transcription factors is essential for controlling physiological functions, metabolism and development in insects. However, the role of the Fox gene in the diamondback moth, <em>Plutella xylostella</em>, a major agricultural pest of cruciferous crops, is still unknown. The purpose of this work was to characterize the <em>PxFox1</em> gene and investigate its role in the reproduction and development of <em>P. xylostella</em>. The <em>Fox</em> gene in <em>P. xylostella (PxFox1</em>) is identified from the <em>P. xylostella</em> genome. The <em>Fox</em> gene was conserved in lepidoptera with the presence of Forkhead domain. <em>PxFox1</em> was expressed in different developmental stages and tissues of <em>P. xylostella</em>, highly in the female adult and integument. The <em>PxFox1</em> knockout mutants were generated by using the CRISPR/Cas9 technique. The significant reduction in the number of eggs and hatching rate was observed in mutants as compared with the wild-type. Meanwhile, an extended larval duration, shortened pupal phase, and reduced adult lifespan were observed in the mutants. Furthermore, <em>PxFox1</em> mutants displayed significant phenotypic abnormalities such as malformed wings, irregular body segmentation, and reduced body size, highlighting the gene’s essential role in the morphological development. These results showed that <em>PxFox1</em> is necessary for <em>P. xylostella</em> development, survival, and reproduction. Targeting <em>PxFox1</em> could provide a potential genetic pest control strategy for managing <em>P. xylostella</em> populations, offering new insights into insect gene regulation for sustainable pest management.</div></div>\",\"PeriodicalId\":16189,\"journal\":{\"name\":\"Journal of insect physiology\",\"volume\":\"165 \",\"pages\":\"Article 104868\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-08-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of insect physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0022191025001222\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022191025001222","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
Roles of Fox gene in the development and reproduction of diamondback moth
The forkhead box (Fox) family of transcription factors is essential for controlling physiological functions, metabolism and development in insects. However, the role of the Fox gene in the diamondback moth, Plutella xylostella, a major agricultural pest of cruciferous crops, is still unknown. The purpose of this work was to characterize the PxFox1 gene and investigate its role in the reproduction and development of P. xylostella. The Fox gene in P. xylostella (PxFox1) is identified from the P. xylostella genome. The Fox gene was conserved in lepidoptera with the presence of Forkhead domain. PxFox1 was expressed in different developmental stages and tissues of P. xylostella, highly in the female adult and integument. The PxFox1 knockout mutants were generated by using the CRISPR/Cas9 technique. The significant reduction in the number of eggs and hatching rate was observed in mutants as compared with the wild-type. Meanwhile, an extended larval duration, shortened pupal phase, and reduced adult lifespan were observed in the mutants. Furthermore, PxFox1 mutants displayed significant phenotypic abnormalities such as malformed wings, irregular body segmentation, and reduced body size, highlighting the gene’s essential role in the morphological development. These results showed that PxFox1 is necessary for P. xylostella development, survival, and reproduction. Targeting PxFox1 could provide a potential genetic pest control strategy for managing P. xylostella populations, offering new insights into insect gene regulation for sustainable pest management.
期刊介绍:
All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.