Journal of Insect Science最新文献_第4页

Confirmation of Xylella fastidiosa (Lysobacterales: Lysobacteraceae) transmission by 8 leafhopper species present in coffee plantations in Costa Rica. 哥斯达黎加咖啡种植园中8种叶蝉传播苛养木杆菌（溶菌门：溶菌科）的确认。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag068

Laura Garita, Ana Mariel Zúñiga-Pereira, Andrés Arias-Paco, William Villalobos, Sofia Carvajal-Rojas, Jose Pablo Álvarez-Mora, Luis Enrique Chaves-González, Isaías Abdallah, Carolina Godoy, Phyllis G Weintraub, Mauricio Montero-Astúa, Carlos Chacón-Díaz

{"title":"Confirmation of Xylella fastidiosa (Lysobacterales: Lysobacteraceae) transmission by 8 leafhopper species present in coffee plantations in Costa Rica.","authors":"Laura Garita, Ana Mariel Zúñiga-Pereira, Andrés Arias-Paco, William Villalobos, Sofia Carvajal-Rojas, Jose Pablo Álvarez-Mora, Luis Enrique Chaves-González, Isaías Abdallah, Carolina Godoy, Phyllis G Weintraub, Mauricio Montero-Astúa, Carlos Chacón-Díaz","doi":"10.1093/jisesa/ieag068","DOIUrl":"10.1093/jisesa/ieag068","url":null,"abstract":"Xylella fastidiosa Wells et al. 1987 is a vector-transmitted plant pathogen of economic importance that is widespread throughout coffee plantations in Costa Rica. Previous studies have shown the existence of a broad diversity of leafhoppers present in different coffee producing regions in Costa Rica. The presence of leafhoppers in coffee plantations with the presence of X. fastidiosa poses their role as potential vectors. To our knowledge, the vector status of leafhoppers present in coffee plantations in Costa Rica has remained unsettled. This work confirmed 8 species of leafhoppers as vectors of X. fastidiosa related to coffee in Costa Rica. Insects were collected in field in the northern part of the Costa Rican Central Valley, and leafhoppers were identified, classified, and grouped for further analysis. All the confirmed vector species belong to the subfamily Cicadellinae (Hemiptera: Auchenorrhyncha), known as sharpshooters. The ability of these specimens to survive on coffee plants for a 72-h period was assessed. In planta transmission assays were performed on X. fastidiosa-free coffee plants under greenhouse conditions. X. fastidiosa was detected in plants after one and a half years, confirming the vector status of leafhoppers. Positive plants remained asymptomatic throughout the experiment. The implication of these species as vectors strengthens surveillance actions in local and international contexts.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13331132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148382092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the core region responsible for the activity of CmVg promoter and its regulatory transcription factor BrC in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae). 稻纵卷叶蝉CmVg启动子及其调控转录因子BrC活性核心区域的鉴定。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag057

Lingling Long, Dong Jin, Hongshuai Gao, Qiao Gao, Wenbing Ding, Lin Qiu, Youzhi Li, Hualiang He

{"title":"Identification of the core region responsible for the activity of CmVg promoter and its regulatory transcription factor BrC in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae).","authors":"Lingling Long, Dong Jin, Hongshuai Gao, Qiao Gao, Wenbing Ding, Lin Qiu, Youzhi Li, Hualiang He","doi":"10.1093/jisesa/ieag057","DOIUrl":"10.1093/jisesa/ieag057","url":null,"abstract":"Insect reproduction depends critically on vitellogenesis, during which vitellogenin (Vg) is synthesized in the female fat body and incorporated into developing oocytes as an essential nutrient source. In this study, the promoter region of the vitellogenin gene (CmVg) of the rice leaf folder (Cnaphalocrocis medinalis) was predicted and cloned. A core active region was delineated within the CmVg promoter, and bioinformatic analysis revealed several predicted cis‑regulatory elements (CREs) for Broad‑Complex (BrC) transcription factors in the core region. Among four BrC protein candidates in the genome of C. medinalis, Cmed074000.1 significantly enhanced the transcriptional activity of CmVg. Disruption of the CRE of Cmed074000.1 strongly reduced the expression level of the reporter. Moreover, RNA interference-mediated knockdown of Cmed074000.1 in adult females significantly down‑regulated CmVg transcriptional levels and severely impaired egg production by 62.24%. This work provides the first evidence of a transcriptional pathway controlling CmVg expression in this pest, C. medinalis, offering novel insights into its reproductive biology and highlighting a potential target for future pest‑management strategies.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13386126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148535943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Midgut microbiota profiling of Blattella germanica (Blattodea: Ectobiidae). 德国Blattella微生物剖析。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag074

Kok Yean Von, Li Lim, Abdul Hafiz Ab Majid

{"title":"Midgut microbiota profiling of Blattella germanica (Blattodea: Ectobiidae).","authors":"Kok Yean Von, Li Lim, Abdul Hafiz Ab Majid","doi":"10.1093/jisesa/ieag074","DOIUrl":"10.1093/jisesa/ieag074","url":null,"abstract":"A detailed understanding of the core microbiome in Blattella germanica is essential for clarifying host-symbiont interactions. Although factors such as diet, developmental stage, and environmental conditions influence cockroach gut microbial communities, their combined effects remain poorly understood. Moreover, most studies rely on whole-gut homogenates, leaving the midgut microbiota largely uncharacterized. Hence, 16S rRNA gene amplicon sequencing was used to characterize and compare the midgut bacterial community structure from 28 samples (including 1 negative control), considering the factors of developmental stage, sex, physiological status, and environmental origin. Microbial community profiling revealed 923 unique genera corresponding to 505 families, 306 orders, 137 classes, and 47 phyla. Bacterial communities belonging to the families Lactobacillaceae, Desulfovibrionaceae, Lachnospiraceae, Dysgonomonadaceae, Christensenellaceae, and Rikenellaceae were consistently present across all treatment groups. Alpha diversity analysis showed significant differences in Shannon diversity, while Simpson and Chao1 diversity indices showed no significant variation. Beta diversity analysis based on UniFrac distances revealed significant differences in microbial community composition across experimental conditions, with stronger separation observed when considering taxa abundance. PERMANOVA indicated that both physiological status and environmental origin significantly shaped community structure, while host sex and developmental stage showed significant effects only in weighted UniFrac distance. Family-level and prevalence analyses consistently demonstrated greater similarity between laboratory-fed and starved groups, whereas field-collected samples exhibited a more distinct and less shared taxonomic profile. Overall, these findings advance current understanding of compartment-specific gut microbiota and underscore the ecological stability of microbiome taxa under diverse conditions.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13555822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing insecticide efficacy: synergistic and sublethal impacts of a pirimicarb-imidacloprid mixture on Myzus persicae Sulzer (Hemiptera: Aphididae). 吡虫威-吡虫啉混合药剂对桃蚜（半翅目：蚜科）的增效和亚致死效应。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag055

Mehri Zargani, Hooshang Rafiee Dastjerdi, Bahram Naseri, Asgar Ebadollahi, Vahid Mahdavi

{"title":"Enhancing insecticide efficacy: synergistic and sublethal impacts of a pirimicarb-imidacloprid mixture on Myzus persicae Sulzer (Hemiptera: Aphididae).","authors":"Mehri Zargani, Hooshang Rafiee Dastjerdi, Bahram Naseri, Asgar Ebadollahi, Vahid Mahdavi","doi":"10.1093/jisesa/ieag055","DOIUrl":"10.1093/jisesa/ieag055","url":null,"abstract":"Aphid resistance to insecticides necessitates management strategies that improve efficacy while minimizing chemical input, including combinations of active ingredients with distinct modes of action. This study evaluated the individual and combined toxicity of pymetrozine, pirimicarb, and imidacloprid against third-instar nymphs and adults of the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Using a 48-h leaf-dip bioassay, insecticides were tested individually and in 1:1 binary combinations, and interactions were quantified using the combination index (CI). Sublethal effects of the most effective mixture were further assessed at the LC30 level (16.424 mg l-1) using age-stage, 2-sex life table analysis and biochemical assays. Among the tested combinations, the imidacloprid-pirimicarb mixture produced the strongest combined effect (CI < 0.5) in both developmental stages, whereas the other mixtures showed antagonistic interactions (CI > 1). This mixture resulted in LC50 values of 39.269 mg l-1 for nymphs and 122.553 mg l-1 for adults, corresponding to substantial reductions in effective concentration and active ingredient requirements compared with single-insecticide treatments. Sublethal exposure significantly (P < 0.05) reduced demographic parameters, including the intrinsic rate of increase (r), net reproductive rate (R0), fecundity, and longevity. Biochemical assays also revealed developmental stage-dependent alterations in acetylcholinesterase, esterase, and total protein levels following exposure to the mixture. Overall, the findings demonstrate that the imidacloprid-pirimicarb combination enhances toxicity against M. persicae under laboratory conditions and may contribute to reduced insecticide input, although additional studies are required to clarify the nature of the interaction and evaluate its applicability under field conditions.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Partial deletion in the cuticular protein gene BmorCPR2 results in a body shape mutant in silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). 家蚕表皮蛋白基因BmorCPR2的部分缺失导致家蚕体型突变。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag089

Juan Sun, Min Liu, Xin Zheng, Gui Ouyang, Anli Chen, Heying Qian

{"title":"Partial deletion in the cuticular protein gene BmorCPR2 results in a body shape mutant in silkworm, Bombyx mori L. (Lepidoptera: Bombycidae).","authors":"Juan Sun, Min Liu, Xin Zheng, Gui Ouyang, Anli Chen, Heying Qian","doi":"10.1093/jisesa/ieag089","DOIUrl":"https://doi.org/10.1093/jisesa/ieag089","url":null,"abstract":"The Bombyx mori L. (Lepidoptera: Bombycidae) is a significant economic insect used for silk production. A novel body shape mutant, stony^sunken (st^sk), that exhibits a sunken intersegmental membrane was isolated from the wild type of st^sk (WT-n08). Investigation indicated that the mutation had no significant effect on its growth and development. To elucidate the molecular mechanism underlying this body shape mutant, genetic analysis, positional cloning, and the CRISPR/Cas9 gene editing system were performed. Genetic analysis demonstrated that the mutant trait in st^sk is controlled by an autosomal recessive gene and follows Mendelian inheritance. Positional cloning showed that a putative cuticular protein gene, BmorCPR2 on chromosome 8, was the candidate gene. Sequencing analysis revealed partial deletion of BmorCPR2 exon 2 and intron 2 sequences occurred and subsequently resulted in the premature termination of gene expression. Knock-out of BmorCPR2 using the CRISPR/Cas9 gene editing system led to the sunken intersegmental membrane phenotype. These findings highlight the essential role of BmorCPR2 in silkworm cuticular formation, providing a foundation for further research on cuticular protein function.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13493190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148794339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating efficacy of oxytetracycline, lincomycin, and tylosin for treatment of clinical European foulbrood disease. 评价土霉素、林可霉素和泰乐素治疗临床欧洲污秽病的疗效。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag082

Midhun S Jose, Harmony Woodrow, Maria Janser, Marina C Bezerra da Silva, Oleksii Obshta, Marcelo P Camilli, Emilio E Tellarini Prieto, Thanuri Edirithilake, Ryan Merkl, Igor Moshynskyy, Elemir Simko, Sarah C Wood

{"title":"Evaluating efficacy of oxytetracycline, lincomycin, and tylosin for treatment of clinical European foulbrood disease.","authors":"Midhun S Jose, Harmony Woodrow, Maria Janser, Marina C Bezerra da Silva, Oleksii Obshta, Marcelo P Camilli, Emilio E Tellarini Prieto, Thanuri Edirithilake, Ryan Merkl, Igor Moshynskyy, Elemir Simko, Sarah C Wood","doi":"10.1093/jisesa/ieag082","DOIUrl":"10.1093/jisesa/ieag082","url":null,"abstract":"Antibiotics have been widely used in North American beekeeping for the last 70 yr, contributing to the emergence of antimicrobial resistance (AMR). European foulbrood (EFB) is a stress-associated, reportedly self-limiting, bacterial disease of honey bee larvae, caused by Melissococcus plutonius (ex-White 1912) Bailey and Collins 1983 (Lactobacillales: Enterococcaceae). Although oxytetracycline (OTC) is the only antibiotic approved for EFB, its continued use in North America requires evidence to justify it, given concerns about AMR despite no confirmed resistance in M. plutonius. Moreover, if AMR emerges in M. plutonius, the efficacy of alternative antimicrobials for treatment of EFB should be determined, such as tylosin (TYL) and lincomycin (LMC), which are approved in North America for managing American foulbrood. Therefore, in this study, we evaluated the effectiveness of OTC, TYL, and LMC in treating colonies affected by a natural outbreak of EFB during spring 2024 in Saskatchewan, Canada. Forty-eight infected colonies were split into 4 groups of 12: 1 sham-treated and 3 treated with either OTC, TYL, or LMC per label instruction. Colony strength and EFB symptoms were monitored over 1 mo, and M. plutonius bacterial load was quantified via Quantitative polymerase chain reaction (qPCR) pretreatment, 1- and 4-wk posttreatment. We found that all antimicrobial-treated colonies had significant improvement in colony strength relative to pretreatment. Adult bees from antimicrobial-treated groups also had a significant reduction in bacterial load 1-mo posttreatment. However, we also observed a spontaneous recovery in sham-treated colonies. Our findings support the clinical efficacy of OTC and highlight the potential of TYL and LMC as effective alternatives for managing EFB.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13431660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148669841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomics and metabolic model analysis of primary and secondary endosymbionts present in different species of whitefly. 不同种类粉虱原生和次生内共生体的比较基因组学和代谢模型分析。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag083

Neeti Pandey, Raman Rajagopal

{"title":"Comparative genomics and metabolic model analysis of primary and secondary endosymbionts present in different species of whitefly.","authors":"Neeti Pandey, Raman Rajagopal","doi":"10.1093/jisesa/ieag083","DOIUrl":"10.1093/jisesa/ieag083","url":null,"abstract":"Candidatus Portiera aleyrodidarum (hereafter Portiera) is the primary endosymbiont of whiteflies (Hemiptera: Aleyrodidae), residing in specialized bacteriocytes that also co-localize diverse secondary endosymbionts. This study aimed to carry out in-depth genomic analysis of different strains of Portiera and secondary endosymbionts namely Arsenophonus, Cardinium, Hamiltonella, Rickettsia, and Wolbachia obtained from different whitefly species (data obtained from NCBI). Interestingly, hierarchical clustering of the amino acid identity data resulted in a dendrogram that resolved the different strains of Portiera into 3 separate genetic clusters. Pan-genome analysis of endosymbionts indicates that even closely related strains of a species, when isolated from different hosts, show unique genomic compositions due to their capability to adapt to a specific environment. COG/KEGG analysis revealed that amino acid metabolism is second most represented functional category of Portiera genomes whereas it is almost negligible in genomic repertoire of secondary endosymbionts except in Hamiltonella. Biosynthetic pathway analysis shows that threonine is the only essential amino acid for which complete set of genes is present in all strains of Portiera followed by leucine and tryptophan which is synthesized by all except 1 (China) and 2 strains (AD-CAI and SiSi), respectively. However, both amino acid lysine and arginine could be synthesized by only 4 strains namely AD-CAI, AF-CAI, PeMo, and TV. Further, the study established that secondary endosymbionts are capable of enriching the host's diet with protective organic compounds, B vitamins, and cofactors which is in contrast to the role of Portiera. Results of pathway-specific metabolic modelling were highly congruent with KEGG pathway predictions, depicting a positive correlation between the genotype and phenotype of endosymbionts.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13452057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148697501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing survival of Leptocybe invasa (Hymenoptera: Eulophidae) parasitoids: effects of nutrition and temperature. 优化入侵小蜂（膜翅目：蜂科）寄生蜂的存活：营养和温度的影响。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag069

Thais Alves da Mota, Amanda Rodrigues de Souza, David Lopes Teixeira, José Raimundo de Souza Passos, Barbara de Oliveira Puretz, José Cola Zanuncio, Carlos Frederico Wilcken

{"title":"Optimizing survival of Leptocybe invasa (Hymenoptera: Eulophidae) parasitoids: effects of nutrition and temperature.","authors":"Thais Alves da Mota, Amanda Rodrigues de Souza, David Lopes Teixeira, José Raimundo de Souza Passos, Barbara de Oliveira Puretz, José Cola Zanuncio, Carlos Frederico Wilcken","doi":"10.1093/jisesa/ieag069","DOIUrl":"10.1093/jisesa/ieag069","url":null,"abstract":"The distribution and economic impact of the invasive gall wasp Leptocybe invasa Fisher & La Salle, 2004 (Hymenoptera: Eulophidae), a major pest of eucalyptus, are global. Quadrastichus mendeli Kim & La Salle, 2008 and Selitrichodes neseri Kelly & La Salle, 2012 (Hymenoptera: Eulophidae) are effective biological control agents of this pest, but their use depends on optimizing laboratory rearing protocols. This study assessed the longevity and survival of Q. mendeli females fed different diets and maintained at temperatures from 18 to 30 °C, and of Q. mendeli females and S. neseri males and females fed different diets at 24 °C. The experiment was conducted in a completely randomized factorial design. Longevity data were analyzed using generalized linear models, and survival was analyzed using Kaplan-Meier survival curves. Longevity and survival varied according to diet and temperature for Q. mendeli females and according to diet at 24 °C for S. neseri males and females. Quadrastichus mendeli females reaching a maximum longevity of 104.7 d with honey diets at moderate temperature. At 24 °C, S. neseri females and males reached maximum longevities of 23.25 d, and 17.00 d, respectively, with honey diets. Longevity declining under starvation or the pollen-only diet. Female longevity exceeded that of males, and Q. mendeli lived longer than S. neseri. Parasitoid longevity and survival were highest with honey diets at moderate temperatures, providing practical conditions for laboratory maintenance, mass rearing, and release of these parasitoids in biological control programs against L. invasa.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13332436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148387655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

eDNA analysis of yard waste samples reveals taxonomical diversity, sequence database limitations, and consistencies across sequencing platforms. 庭院废弃物样本的eDNA分析揭示了分类多样性、序列数据库的局限性和跨测序平台的一致性。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag062

William B Walker, Lisa G Neven

{"title":"eDNA analysis of yard waste samples reveals taxonomical diversity, sequence database limitations, and consistencies across sequencing platforms.","authors":"William B Walker, Lisa G Neven","doi":"10.1093/jisesa/ieag062","DOIUrl":"10.1093/jisesa/ieag062","url":null,"abstract":"Timely identification of biological species is often needed for various purposes, including economic reasons, and advances in DNA sequencing technologies have greatly augmented the ability to identify species through the application of DNA barcoding. One such method examines environmental DNA (eDNA) to sample the presence of organisms in an environment without necessarily having direct access to the whole organisms. In recent years, multiple high-throughput sequencing platforms have emerged, and there are differences in the efficiency, effectiveness, and economics across these platforms. In this report, we examine the application of two platforms, from PacBio and Oxford Nanopore Technologies, to sequence COI amplicons from nine barcoded yard waste samples that we previously studied for a different purpose. Here, we observed consistencies across the platforms in the identification of operational taxonomical units (OTUs) from broad swaths of life, most prominently including Bacteria, Amoebozoa, Fungi, Arthropoda, Nematoda, Spiralia, and Viridiplantae. Other taxonomical groupings were also tentatively identified. However, limitations in coverage of the diversity of COI sequences in the public databases rendered species-level identification impossible for many of the OTUs. Insect species were the best represented across all barcoded samples, and both sequencing platforms regarding percentage identity to the best BLAST hits in the databases. Following this, we took an in-depth look at the knowledge of the presence of highly matched species in the locality from where the eDNA samples were derived. Strengths and limitations of this approach in the analysis of eDNA are discussed.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13342719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of rose pollen and nectar on body size and ovarian development of Frankliniella occidentalis (Thysanoptera: Thripidae). 玫瑰花粉和花蜜对西花蓟马体型和卵巢发育的影响。

IF 2.3 3区农林科学

Journal of Insect Science Pub Date : 2026-07-01 DOI: 10.1093/jisesa/ieag066

Xin Lei Fang, Jun Rui Zhi, Wen Bo Yue

{"title":"Effects of rose pollen and nectar on body size and ovarian development of Frankliniella occidentalis (Thysanoptera: Thripidae).","authors":"Xin Lei Fang, Jun Rui Zhi, Wen Bo Yue","doi":"10.1093/jisesa/ieag066","DOIUrl":"10.1093/jisesa/ieag066","url":null,"abstract":"Western flower thrips, Frankliniella occidentalis (Pergande), is an omnivorous pest feeding on plant tissues and floral resources, including pollen and nectar, and can consume mite eggs. Its growth and reproduction are strongly influenced by diet quality. Compared with rose petals alone, a standardized whole-flower diet contains reproductive organs and therefore provides additional floral resources. In this study, a standardized whole-flower diet, rose petals, and kidney bean leaves as a host-plant control were used to investigate whether differences in floral resource availability affect thrips development and reproduction. After three consecutive generations, multiple parameters were measured, including body size (length and width), ovarian development (ovariole length and number of mature eggs), juvenile hormone (JH) content, vitellogenin (Vg) content, and expression of Kr-h1, br, JHEH, and Vg. Relative to the kidney bean leaf control, thrips on the whole-flower diet exhibited significantly greater body length and width, whereas those on rose petals had shorter length and similar width. Reproductive traits varied with adult age, and females reared on the whole-flower diet generally had longer ovarioles than those reared on rose petals and significantly greater numbers of mature eggs than the kidney bean leaf control at each sampled time point from Days 2 to 9 after adult emergence. JH and Vg contents and gene expression also varied significantly with diet, adult age, and their interaction. Together, these findings indicate that floral resources in the whole-flower diet enhance the growth and reproductive development of F. occidentalis and may favor its performance on flowering host plants.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13387359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148368674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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