Journal of Insect Science最新文献

{"title":"Genetic insights into the first detection of Paracoccus marginatus (Hemiptera: Pseudococcidae) in Australia.","authors":"Sonu Yadav, Shreya Patel, Sachinthi Kithulgoda, Haidee Brown, Brian Thistleton","doi":"10.1093/jisesa/ieae118","DOIUrl":"10.1093/jisesa/ieae118","url":null,"abstract":"<p><p>Species spread in a new environment is often associated with founders' effect, and reduced effective population size and genetic diversity. However, reduced genetic diversity does not necessarily translate to low establishment and spread potential. Paracoccus marginatus Williams and Granara de Willink is a polyphagous pest that has invaded 4 continents in around 34 years. It was first detected in the Northern Territory, Australia in July 2023. Following this, we collected 45 specimens from 20 suburbs across 3 regions. Using mitochondrial cytochrome oxidase I and nuclear ribosomal RNA genes (18S and 28S), we examined molecular diagnostics robustness, genetic diversity, haplotype network, and demographics (Tajima D) of the recently detected Australian population. We compared our samples with publicly available sequences deposited on GenBank. All 3 genes were suitable for molecular diagnosis with a 100% identity score. For all 3 genes, we found low nucleotide diversity, haplotype diversity, and negative Tajima D in the newly detected P. marginatus population, suggesting a recent single founder event by a few individuals. Comparing our study's sequences with global sequences showed low haplotype diversity, nucleotide diversity, and Tajima's D, suggesting that despite low genetic diversity at the 3 genes, P. marginatus has successfully invaded South America, Africa, Asia, and Oceania. Our study highlights the role of demographic and life history traits in the species' invasion success. We provide a baseline dataset from the first detection of P. marginatus in Australia. Further analysis of the spread can provide insights into invasive species' adaptation in a novel environment.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770599/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xylosandrus ambrosia beetles preference of nursery tree species for attacks and colonization under water stress.","authors":"Madhav Parajuli, Cansu Oksel, Krishna Neupane, Christopher M Ranger, Fulya Baysal-Gurel","doi":"10.1093/jisesa/ieaf003","DOIUrl":"10.1093/jisesa/ieaf003","url":null,"abstract":"<p><p>The role of flood and drought stress on Xylosandrus ambrosia beetle attacks and colonization in nursery trees with varying levels of water stress tolerance has not yet been studied. This study aimed to examine ambrosia beetle preference for tree species varying in their tolerance to water stress. Container-grown dogwoods, redbuds, and red maples were exposed to flood, drought, or sufficient water treatments for 28 d and beetle attacks were counted every third day. Stem tissues were collected at 7 and 14 d after imposing water stress to measure ethanol content using SPME-GC-MS. Trees were dissected to record gallery formation, fungal colonization, presence of eggs, larvae, and adults within the gallery. Additionally, gallery depth was measured. Under flood stress, beetle attacks were found exclusively in dogwood and redbud, with no attacks in red maple. There was a higher number of attacks in dogwood compared to redbud. Gallery formation and depth, fungal colonization, and presence of eggs, larvae, and adults in galleries were higher in dogwood than redbud. About 85% of the adults recovered from galleries were X. crassiusculus and the rest were X. germanus. Among the trees subjected to drought stress, only one dogwood tree had attacks, while the redbuds and red maples had no beetle attack. Tree species assigned to sufficient water were not attacked. Higher ethanol content was detected in dogwood and redbud subjected to flood stress compared to flooded red maples and tree species assigned to drought and sufficient water. This understanding can help growers and landscapers in making informed management decisions.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moth caterpillar embryos and parasitoid egg infection as revealed in vivo and visualized by micro-CT scanning.","authors":"Ryan J Smith, Liwen Han, Jacqueline C Bede, Pierre Dutilleul","doi":"10.1093/jisesa/ieae114","DOIUrl":"10.1093/jisesa/ieae114","url":null,"abstract":"<p><p>The Lepidopteran pest Trichoplusia ni and the parasitoid wasp Trichogramma brassicae represent a fascinating biological system, important for sustainable agricultural practices but challenging to observe. We present a nondestructive method based on micro-CT scanning technology (CT: computed tomography) for visualizing the internal parts of caterpillar embryos and of emerging parasitoids from infected eggs. Traditional methods of microscopic observation of the opaque egg contents require staining or dissection. To explore the biological system nondestructively, we optimized the application of micro-CT scanning to construct 3-D images of insects in vivo.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Behavioral response of house flies (Diptera: Muscidae) to 3 bittering agents.","authors":"Panchalie B Gunathunga, Bethia H King, Christopher J Geden","doi":"10.1093/jisesa/ieae117","DOIUrl":"10.1093/jisesa/ieae117","url":null,"abstract":"<p><p>House flies, Musca domestica L., are pests at livestock facilities. House fly numbers are controlled mainly by manure management and chemical controls, like use of toxic baits. Some toxic baits contain denatonium benzoate as a bittering agent, to avoid poisoning of children. House fly behavioral response to denatonium benzoate was examined, using 2 different diluents, sucrose solution or orange juice. Response of flies to quinine and sucrose octaacetate, 2 other bittering agents, was also examined in sucrose. Sucrose is the sweetener in commercially available toxic house fly baits. The proboscis extension response of both male and female flies was significantly reduced by addition of 1,000 ppm of denatonium benzoate to sucrose. Response in males was also reduced at 100 ppm. Consumption of sucrose by both male and female flies was reduced by addition of 100, 500, and 1,000 ppm of denatonium benzoate, but consumption by males was also reduced at 10 ppm. Addition of sucrose octaacetate had no detectable effect on consumption of sucrose at 100, 500, or 1,000 ppm. In contrast, addition of quinine reduced consumption by both male and female flies in all tested concentrations. In orange juice, 10 ppm of denatonium benzoate is often avoided by children. With orange juice, fly proboscis extension response patterns were the same for both sexes, with a reduction at 1,000 ppm of denatonium benzoate. Orange juice consumption by flies was reduced significantly at 1,000 ppm of denatonium benzoate for females but not at any of the concentrations tested for males.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11792078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical compounds associated with Schistocerca piceifrons piceifrons (Orthoptera: Acrididae), by Gas Chromatography-Mass Spectrometry analysis.","authors":"Luis Daniel García-García, Ludivina Barrientos-Lozano, Jorge Ariel Torres-Castillo, Aurora Y Rocha-Sánchez, Pedro Almaguer-Sierra, Othón Javier González-Gaona, José Rodríguez-Rodríguez","doi":"10.1093/jisesa/ieaf011","DOIUrl":"10.1093/jisesa/ieaf011","url":null,"abstract":"<p><p>Semiochemicals are chemicals that mediate inter- and intraspecific interactions between insects. Recently, these chemicals have begun to be investigated as an alternative for the management of the Central American locust, Schistocerca piceifrons piceifrons (Walker, 1870), a pest that can cause substantial damage to crops, pastures, and native vegetation. A suitable method for investigating the range of semiochemicals present in Central American locust is surface washing in organic solvents. In the work described here, the extraction of chemical compounds was carried out with hexane in a 1:10 (w/v) ratio, in 2 resting periods (1 and 24 h) and 2 stages of Central American locust development (young adult and adult in reproductive stage). By Gas Chromatography-Mass Spectrometry, 17 chemical compounds previously identified with semiochemical potential in other insects were detected. The study of semiochemicals in Central American locust offers the opportunity to identify specific chemical compounds that may have the potential to implement management alternatives for this pest. Specific studies of each compound detected in this work are needed to elucidate whether they have semiochemical activity and potential to combat the pest and reduce the environmental damage caused by the application of synthetic chemical products.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11815486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-sample long-read nanopore sequencing of Agabus bipustulatus (Coleoptera: Dytiscidae: Agabinae) mitogenome produces effectively reliable data for downstream analyses.","authors":"Olena Bielikova, Ondrej Vargovčík, Zuzana Čiamporová-Zaťovičová, Fedor Čiampor","doi":"10.1093/jisesa/ieaf009","DOIUrl":"10.1093/jisesa/ieaf009","url":null,"abstract":"<p><p>Mitochondrial genomes are a rich source of data for various downstream analyses such as population genetics, phylogeny, and systematics. Today it is possible to assemble rapidly large numbers of mitogenomes, mainly employing next-generation sequencing and third-generation sequencing. However, verification of the correctness of the generated sequences is often lacking, especially for noncoding, length-variable parts. Here we have assembled the mitochondrial genome (mitogenome) from four specimens of Agabus bipustulatus (L.) using long-read nanopore sequence data. The use of the latest nanopore chemistry (V14) combined with a comprehensive error correction workflow enabled the generation of mitogenomes with high accuracy and reproducibility, as tested on four samples. The resulting mitogenome is 17,876 bp long, including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a control region. Differences in the control region length between samples were minimal. The arrangement of protein-coding genes, transfer RNAs, and ribosomal RNAs is similar to that of the ancestral insect mitogenome. Finally, we used the assembled, well-supported mitogenomes in the phylogenetic analysis of a part of the Dytiscidae related to the studied species and confronted the results with previous hypotheses. Conflicting estimates of their phylogeny suggest that considerably more robust data are required for a plausible sketch of their evolutionary history. Our research has confirmed that readily available third-generation sequencing technologies, such as Oxford Nanopore Technologies, combined with long-read sequencing, offer a highly efficient, reliable, and cost-effective approach to generate complete mitogenomes and potentially other longer regions of the genome. The use of reliable data will ultimately contribute to a deeper understanding and improved conservation strategies for diving beetles and other organisms.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11780178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing cultivar-specific susceptibility and morphological adaptations of Bactrocera oleae (Diptera: Tephritidae) in olive orchards.","authors":"Ivana Pajač Živković, Dana Čirjak, Luka Hojsak, Barbara Vrček, Manuel J Suazo, Hugo A Benitez, Darija Lemic","doi":"10.1093/jisesa/ieaf005","DOIUrl":"10.1093/jisesa/ieaf005","url":null,"abstract":"<p><p>The olive fruit fly, Bactrocera oleae (Rossi), is the most important widespread pest in olive-growing areas worldwide, causing significant yield losses and deterioration of olive oil quality. This study hypothesized that olive cultivars differ in their susceptibility to B.oleae and that the cultivar in which the pest develops may affect population variability. The primary goal was to assess susceptibility across 6 olive cultivars, while the secondary goal was to analyze population variability using geometric morphometrics to identify cultivar-specific phenotypic differences. Conducted at 2 sites, Banjevci (Dalmatia) and Vodnjan (Istria), the study revealed significant differences in infestation levels, emergence, and wing morphology. Higher humidity in Vodnjan favored increased fly populations. Larger-fruited cultivars such as Oblica, Istarska bjelica, and Ascolana tenera experienced higher infestation rates, whereas smaller-fruited cultivars like Frantoio and Leccino exhibited lower infestation levels. Wing shape analysis highlighted sexual dimorphism, with female flies exhibiting broader wings that may enhance dispersal and oviposition efficiency, particularly in larger fruits. The observed phenotypic plasticity of B. oleae across cultivars and locations indicates an ability to adapt to varied host and cultivation conditions, enhancing pest resilience. The findings underscore the role of cultivar selection as a passive pest management strategy and demonstrate the utility of geometric morphometrics in capturing cultivar-specific morphological adaptations.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11815492/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The conserved IR75 subfamily mediates carboxylic acid detection in insects of public health and agricultural importance.","authors":"Matthew Cooke, Michael S Chembars, Ronald Jason Pitts","doi":"10.1093/jisesa/ieaf012","DOIUrl":"10.1093/jisesa/ieaf012","url":null,"abstract":"<p><p>Insects perceive and respond to carboxylic acids (CAs), amines, and aldehydes primarily via conserved ionotropic receptors (IRs). These receptors form the basis for a second olfactory system distinct from the well-characterized odorant receptors. Neurons expressing IRs are housed in dedicated sensilla and innervate glomeruli, separate from those innervated by odorant receptor neurons. The IR8a co-receptor is highly conserved across insect orders and, together with ionotropic receptor tuning receptors, primarily detects CAs. The conservation of genes and the anatomical separation of neural pathways underscore the importance of these compound classes and CAs, specifically in insect chemical ecology. We provide a summary of carboxylic acid detection in insects, focusing on dipteran and lepidopteran species of significance to public health and agriculture. An overview of insect behavior toward CAs is provided, as well as a comprehensive update on carboxylic acid receptor function in insects. Phylogenetic analysis of publicly available genome databases reveals several species that encode and express homologs of previously deorphanized carboxylic acid receptors, highlighting avenues for future research.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11785732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measuring the effect of RFID and marker recognition tags on cockroach (Blattodea: Blaberidae) behavior using AI-aided tracking.","authors":"Callum J McLean, David N Fisher","doi":"10.1093/jisesa/ieaf002","DOIUrl":"10.1093/jisesa/ieaf002","url":null,"abstract":"<p><p>Radio frequency identification (RFID) technology and marker recognition algorithms can offer an efficient and non-intrusive means of tracking animal positions. As such, they have become important tools for invertebrate behavioral research. Both approaches require fixing a tag or marker to the study organism, and so it is useful to quantify the effects such procedures have on behavior before proceeding with further research. However, frequently studies do not report doing such tests. Here, we demonstrate a time-efficient and accessible method for quantifying the impact of tagging on individual movement using open-source automated video tracking software. We tested the effect of RFID tags and tags suitable for marker recognition algorithms on the movement of Argentinian wood roaches (Blapicta dubia, Blattodea: Blaberidae) by filming tagged and untagged roaches in laboratory conditions. We employed DeepLabCut on the resultant videos to track cockroach movement and extract measures of behavioral traits. We found no statistically significant differences between RFID tagged and untagged groups in average speed over the trial period, the number of unique zones explored, and the number of discrete walks. However, groups that were tagged with labels for marker recognition had significantly higher values for all 3 metrics. We therefore support the use of RFID tags to monitor the behavior of B. dubia but note that the effect of using labels suitable for label recognition to identify individuals should be taken into consideration when measuring B.dubia behavior. We hope that this study can provide an accessible and viable roadmap for further work investigating the effects of tagging on insect behavior.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11760971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A female sterilization method for use in field-based behavioral studies of the invasive Asian longhorned beetle (Anoplophora glabripennis).","authors":"Jennifer L Chandler, Robert Talbot Trotter","doi":"10.1093/jisesa/ieae119","DOIUrl":"10.1093/jisesa/ieae119","url":null,"abstract":"<p><p>Asian longhorned beetle (Anoplophora glabripennis Motschulsky), a wood borer (Coleoptera: Cerambycidae) native to China, has been unintentionally and repeatedly introduced to North American and European landscapes as a stow-away in the wood packing material commonly used in international trade. Asian longhorned beetle causes extensive damage and mortality in multiple deciduous tree species and in response, countries in both North America and Europe have adopted policies of eradication. Models that integrate patterns of Asian longhorned beetle dispersal with records of infested trees are critical in optimizing survey and eradication efforts and tracking eradication progress. While these tools continue to be developed, they have been limited by the availability of experimental dispersal data. Existing data is restricted to observations made in the beetle's native range in China or based on inference of dispersal in invaded landscapes. Direct observation of beetle dispersal behavior in invaded landscapes could provide critical behavioral information, but the experimental release of gravid females has been incompatible with eradication program efforts. To fill this knowledge gap, there is a need to identify field-portable methods of effectively sterilizing mated females that do not alter ovipositional behavior. Here, we present a protocol for cauterizing a beetle's ovipositor to prevent successful oviposition. Results of lab trials demonstrate the efficacy of ovipositor cauterization in inhibiting successful oviposition without altering the egg-laying behavior of gravid Asian longhorned beetle females. This method enables research to inform models of beetle dispersal and infestation risk without adding to actual or perceived risk of exacerbating infestations in an eradication program.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}