Journal of Insect Science最新文献

Changes in life history and morphological traits over 8 generations in the brown marmorated stink bug (Hemiptera: Pentatomidae) under mass-rearing conditions. 大规模饲养条件下褐纹蝽8代生活史和形态特征的变化

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf054

Giacomo Bulgarini, Clara Frasconi Wendt, Manfred Wolf, Angelika Gruber, Leonardo Calabrò, Antonio Pignalosa, Stefanie Fischnaller

{"title":"Changes in life history and morphological traits over 8 generations in the brown marmorated stink bug (Hemiptera: Pentatomidae) under mass-rearing conditions.","authors":"Giacomo Bulgarini, Clara Frasconi Wendt, Manfred Wolf, Angelika Gruber, Leonardo Calabrò, Antonio Pignalosa, Stefanie Fischnaller","doi":"10.1093/jisesa/ieaf054","DOIUrl":"10.1093/jisesa/ieaf054","url":null,"abstract":"Developing and establishing a permanent insect population under mass-rearing conditions is challenging, but it offers the opportunity to collect and compare life history, physiological, morphological, and behavioral traits in real-time and over multiple generations. Halyomorpha halys (Stål, 1855) (Hemiptera: Pentatomidae), a serious agricultural insect pest in northern Italy, was used to establish a permanent mass-rearing protocol under controlled abiotic conditions. The study aimed to evaluate the impact of permanent laboratory rearing on various life history and morphological traits over 8 generations. Development time and developmental success rate of the eggs and nymphal stages, fecundity, mortality rate and body size of the adults were documented. In general, a significant variability was observed in both developmental success rate and developmental time for eggs and juvenile stages, although without an obvious trend. In adults, on the other hand, a common trend in fecundity, number of egg masses and survival was observed. All 3 parameters exhibited a marked decline beginning in the second generation, followed by a significant recovery starting from the seventh generation, indicating potential laboratory adaptation. The body size, on the other hand, showed a slight decrease from the second generation that remained almost constant in subsequent generations. While the results demonstrate the clear success of a continuous H. halys mass-rearing, they also show the current challenges and limits of rearing this invasive insect species under laboratory conditions over several generations without the addition of new individuals.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling spatial acuity improves trap capture of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae). 空间灵敏度模型提高了西部花蓟马Frankliniella occidentalis（蓟翅目：蓟科）的捕获。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf049

Natalie S Roberts, Madelyn Jones, Farooq Shah, Tariq M Butt, William L Allen

{"title":"Modeling spatial acuity improves trap capture of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae).","authors":"Natalie S Roberts, Madelyn Jones, Farooq Shah, Tariq M Butt, William L Allen","doi":"10.1093/jisesa/ieaf049","DOIUrl":"10.1093/jisesa/ieaf049","url":null,"abstract":"Colored sticky traps are used for management of many common agricultural insect pests. Several recent studies have shown that traps can be improved by systematically considering properties of color vision for the target species. In the current study, we extend this approach to spatial vision, using information about the interommatidial angle of an agriculturally important insect pest, western flower thrips Frankliniella occidentalis (Pergande), to predict spatial resolution capabilities for a yellow flower pattern across a range of viewing distances. We tested the hypothesis that pattern sizes matching the spatial resolution capabilities of western flower thrips at a given viewing distance would outperform traps with mismatched pattern sizes by measuring the number of western flower thrips caught on sticky traps containing differently sized flower patterns resolvable at 5, 10, or 20 cm. We found an interaction between pattern size and viewing distance, with significantly more western flower thrips caught on traps when the predicted resolvable distance of the pattern matched the distance traps were placed from a central release point. We further tested the range over which trap patterns are effective in more complex viewing environments using commercial polytunnels. In polytunnel trials, we found that increasing the resolvable distance of patterns increased western flower thrips capture up to approximately 26 cm, after which western flower thrips capture decreased up to the maximal visible range tested (50 cm) in the absence of additional sensory cues. Together, these results show the utility of considering spatial vision in improving trap performance and offers functional insights to improve pest management in visual trap design.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The biological characteristics and life table parameters of Plodia interpunctella (Lepidoptera: Pyralidae) reared on different maize varieties. 不同玉米品种饲养点间倍蛾的生物学特性及生命表参数。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf047

Zahra Saeedi, Masumeh Ziaee, Mehdi Esfandiari, Somaiyeh Ghasemzadeh

{"title":"The biological characteristics and life table parameters of Plodia interpunctella (Lepidoptera: Pyralidae) reared on different maize varieties.","authors":"Zahra Saeedi, Masumeh Ziaee, Mehdi Esfandiari, Somaiyeh Ghasemzadeh","doi":"10.1093/jisesa/ieaf047","DOIUrl":"10.1093/jisesa/ieaf047","url":null,"abstract":"The Indianmeal moth, Plodia interpunctella (Hübner) is a major polyphagous pest of stored food products causing serious quantity and quality losses. In this study, the life history of P. interpunctella was evaluated on different maize varieties, including Simon, Valbom, 703, BK, and BC678. The preadult duration for P. interpunctella were 35.5, 43.1, 39.2, 43.4, and 36.8 d on Simon, Valbom, 703, BK, and BC678, respectively. The mean total longevity on Valbom was 52.2 d which was significantly longer than the 41.8 d on Simon (P = 0.012). The developmental period of moths was the most prolonged on Valbom indicating low nutritional suitability of this variety. However, the moths preferred Valbom for oviposition, and more than 132 eggs were laid on this variety. The most intrinsic rate of increase (r) was reported on BC678 and Simon, while the lowest population growth rate was on BK and Valbom. The highest r value on BC678 and Simon could be due to their high moisture and protein content. Based on the shorter preadult, total preoviposition period, mean generation time and higher life table parameters (gross reproductive rate, r, and λ) that occurred on the BC678, make this variety most favorable host for P. interpunctella. The findings highlighted the importance of maize variety selection in managing this pest in stored food products.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Bactrocera minax (Diptera: Tephritidae) adult population density on its marginal distribution. 小小实蝇成虫密度对其边缘分布的影响。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf028

Yibing Guo, Bo Xu, Cong Huang, Huimin Yang, Fulian Wang, Lianyou Gui, Guifen Zhang

{"title":"Effect of Bactrocera minax (Diptera: Tephritidae) adult population density on its marginal distribution.","authors":"Yibing Guo, Bo Xu, Cong Huang, Huimin Yang, Fulian Wang, Lianyou Gui, Guifen Zhang","doi":"10.1093/jisesa/ieaf028","DOIUrl":"10.1093/jisesa/ieaf028","url":null,"abstract":"In order to explore the impact of insect density on the edge effect of Bactrocera minax (Diptera: Tephritidae) adult distribution in orange orchards, traps were set up in orchards with maggot infestation rates of 2%, 4%, and 20% to attract adults. The study compared differences in distribution between the side with noncitrus trees and the side farther away from them. The results showed that at lower insect densities (2% and 4% maggot infestation rates), the proportion of insect trap sites and the number of insects per trap on the side of the orchard adjacent to the trees were significantly higher than that on the side away from the noncitrus trees, additionally, the proportion of adults captured 10 to 20 m away from the side of noncitrus trees was also significantly higher than at other distances. However, at higher insect density (20% maggot infestation rates), there were no significant differences in the proportion of insect traps or the number of insects per trap between the adjacent and distant sides of the trees. Similarly, there were no significant differences in the proportion of adults captured at distances of 10, 20, 30, and 40 m away from the side of noncitrus trees in the orchard. In summary, at low insect density, B. minax adults exhibit a strong edge effect, concentrating on the side of the orchard with noncitrus trees, whereas at high insect density, they are evenly distributed throughout the orchard.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longevity, feeding behavior, and egg production of adult house flies (Diptera: Muscidae) provided with potential foods on dairy farms. 为奶牛场提供潜在食物的成年家蝇（双翅目：蝇科）的寿命、摄食行为和产卵量。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf045

Anna E Grimenstein, Melissa A Doyle, Bethia H King, Chris J Geden

{"title":"Longevity, feeding behavior, and egg production of adult house flies (Diptera: Muscidae) provided with potential foods on dairy farms.","authors":"Anna E Grimenstein, Melissa A Doyle, Bethia H King, Chris J Geden","doi":"10.1093/jisesa/ieaf045","DOIUrl":"https://doi.org/10.1093/jisesa/ieaf045","url":null,"abstract":"Adult house flies (Musca domestica L.) are often a major pest at livestock facilities, where oviposition occurs on decomposing organic matter, such as manure. Some potential foods that adult house flies might consume on dairy farms were examined. Relative to when they were given water alone, survival of males and females was greater when they were given water along with liquid whole milk, formulated calf feed, or corn silage, or finely milled sorghum or soy, or buckwheat inflorescences. However, survival was significantly lower with these foods than with sucrose, although not significantly so for males with milk. There was little to no survival advantage when flies were given water along with milled hominy, wet spent brewers grain, or manure than with water alone. Both males and females spent significant time with their labellum in contact with corn silage, dandelion inflorescences, and buckwheat inflorescences, but little time with their labellum contacting manure or white clover inflorescences. Egg production was not increased by access to water along with buckwheat inflorescences or corn silage relative to sucrose; but egg production was increased by access to liquid whole milk. Reaching mature vitellogenesis stages was improved by prior exposure to water and sucrose solution along with a mixture of dry sucrose, milk, and yolk, or along with calf manure or formulated calf feed, but not with milled soy, sorghum, or hominy, or with wet spent brewers grain or citrus pellets. The diet including sucrose-milk-yolk resulted in the most females reaching mature vitellogenesis stages.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness and the synergism effects of Ipomoea cairica leaf plant extract and Metarhizium anisopliae fungi (Meta-G4) against larvae of Aedes aegypti Linnaeus and Aedes albopictus Skuse (Diptera: Culicidae). 金龟子叶提取物与金龟子绿僵菌真菌（Meta-G4）对埃及伊蚊和白纹伊蚊幼虫的防效及增效作用

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf044

Kin Hoong Ng, Wan Fatma Zuharah

{"title":"Effectiveness and the synergism effects of Ipomoea cairica leaf plant extract and Metarhizium anisopliae fungi (Meta-G4) against larvae of Aedes aegypti Linnaeus and Aedes albopictus Skuse (Diptera: Culicidae).","authors":"Kin Hoong Ng, Wan Fatma Zuharah","doi":"10.1093/jisesa/ieaf044","DOIUrl":"10.1093/jisesa/ieaf044","url":null,"abstract":"Insecticide resistance is a major public health concern. Biopesticides, derived from naturally occurring substances such as plant extracts and fungal metabolites, are utilized as natural control agents against mosquito vectors. This study focuses on biopesticides to reduce chemical insecticide use in Penang Island, Malaysia. Ipomoea cairica Linnaeus Sweet (Solanales: Convolvulaceae) leaf extracts, Metarhizium anisopliae s.l. (Metsch) Sorok (Ascomycota: Clavicipitaceae) strain Meta-G4, and synergistic effects of both agents were demonstrated against Aedes albopictus Skuse (Diptera: Culicidae) and Aedes aegypti Linnaeus (Diptera: Culicidae) using larvicidal bioassays. Before assessing synergism, the compatibility of both agents was performed to minimize inhibition of mycelial growth on potato dextrose agar. The results showed that Ae. aegypti field strains (urban and suburban) are significantly more susceptible to I. cairica leaf extracts compared to Ae. albopictus field strains (urban and suburban) due to the lower lethal concentrations (LC50 and LC95). Aedes albopictus suburban field strain is significantly more susceptible than other strains when tested with M. anisopliae due to the lower LC50. The combination of M. anisopliae (1 × 106 conidia/ml) with I. cairica leaf extracts (350 ppm) achieved the lowest LT50 and LT95 against Ae. aegypti urban field strain. This study indicates that the synergistic combination of both agents exhibits significant larvicidal efficacy and holds promise for future biological control strategies targeting Aedes populations.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defining the decline: a glossary relevant to insect decline. 定义衰退：与昆虫衰退相关的词汇。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf048

Jessica Awad, Gagandeep Brar, Erin Cadwalader, DeShae Dillard, Lauren A Esposito, Elaine Evans, Christina Grozinger, Kelsey E Fisher, Akito Y Kawahara, Christian H Krupke, Andrea Lucky, Richard Mankin, Corrie S Moreau, Avalon C S Owens, Emily L Sandall, Katja C Seltmann, Jessica L Ware, Ross Winton

{"title":"Defining the decline: a glossary relevant to insect decline.","authors":"Jessica Awad, Gagandeep Brar, Erin Cadwalader, DeShae Dillard, Lauren A Esposito, Elaine Evans, Christina Grozinger, Kelsey E Fisher, Akito Y Kawahara, Christian H Krupke, Andrea Lucky, Richard Mankin, Corrie S Moreau, Avalon C S Owens, Emily L Sandall, Katja C Seltmann, Jessica L Ware, Ross Winton","doi":"10.1093/jisesa/ieaf048","DOIUrl":"10.1093/jisesa/ieaf048","url":null,"abstract":"Insects are declining in abundance and species richness, globally. This has broad implications for the ecology of our planet, many of which we are only beginning to understand. Comprehensive, large-scale efforts are urgently needed to quantify and mitigate insect biodiversity loss. Because there is broad interest in this topic from a range of scientists, policymakers, and the general public, we posit that such endeavors will be most effective with precise and standardized terms. The Entomological Society of America is the world's largest association of professional entomologists and is ideally positioned to lead the way on this front. We provide here a glossary of definitions for biodiversity loss terminology. This can be used to enhance and clarify communication among entomologists and others with an interest in addressing the multiple overlapping research, policy, and outreach challenges surrounding this urgent issue.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Success of parasitism of Nezara viridula and Halyomorpha halys (Hemiptera: Pentatomidae) by Trichopoda pennipes (Diptera: Tachinidae) in the southeastern United States. 美国东南部刺毛蛾（双翅目：刺毛蛾科）寄生刺毛蛾（半翅目：刺毛蛾科）的成功

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf046

P Glynn Tillman, Erin E Grabarczyk

{"title":"Success of parasitism of Nezara viridula and Halyomorpha halys (Hemiptera: Pentatomidae) by Trichopoda pennipes (Diptera: Tachinidae) in the southeastern United States.","authors":"P Glynn Tillman, Erin E Grabarczyk","doi":"10.1093/jisesa/ieaf046","DOIUrl":"10.1093/jisesa/ieaf046","url":null,"abstract":"Nezara viridula (L.) and Halyomorpha halys (Stål) are stink bug pests that damage fruit in a variety of crops throughout the southeastern United States. Trichopoda pennipes (F.) parasitizes N. viridula late instars and adults, however, little is known regarding parasitism of H. halys by this tachinid in systems where both stink bug species coexist. Thus, our main objective was to analyze patterns of T. pennipes parasitism for adult N. viridula and H. halys in corn, cotton, and soybean. We also assessed location of T. pennipes eggs on stink bug bodies as well as successful emergence from parasitized adults (ie at least 1 parasitoid egg per host body). Overall, the proportion of parasitized adults was higher for N. viridula compared to H. halys and higher in corn and cotton compared to soybean. Males were parasitized more often than females for both species. For N. viridula, T. pennipes developed to adults more often when eggs were laid on the ventral thorax. Supernumerary oviposition by T. pennipes was evident for both species. Even though T. pennipes readily oviposited on H. halys, new adults did not emerge from parasitized adults. For N. viridula, mortality of first instar T. pennipes was high due to their difficulty in entering the body; consequently, larval competition within the body was minimal. Adult T. pennipes emerged more often when two to four eggs were deposited on an adult stink bug. We conclude that T. pennipes prefers N. viridula as a host. Therefore, conservation biocontrol is an important management strategy for this pest.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Krüppel homolog 1 mediates juvenile hormone action to suppress photoperiodic reproductive diapause-related phenotypes in the female Chrysoperla nipponensis (Neuroptera: Chrysopidae). kr<s:1> ppel同源物1介导幼体激素作用，抑制雌性日本蝶（Chrysoperla nipponensis）的光周期生殖滞育相关表型。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-03-14 DOI: 10.1093/jisesa/ieaf027

Haiyi Huang, Dandan Li, Minghui Xu, Shaofeng Zhong, Shaoye Liu, Xingke Gao, Yongyu Xu, Zhenzhen Chen

{"title":"Krüppel homolog 1 mediates juvenile hormone action to suppress photoperiodic reproductive diapause-related phenotypes in the female Chrysoperla nipponensis (Neuroptera: Chrysopidae).","authors":"Haiyi Huang, Dandan Li, Minghui Xu, Shaofeng Zhong, Shaoye Liu, Xingke Gao, Yongyu Xu, Zhenzhen Chen","doi":"10.1093/jisesa/ieaf027","DOIUrl":"10.1093/jisesa/ieaf027","url":null,"abstract":"Juvenile hormone (JH) has been revealed to be a critical factor in regulating photoperiod reproductive diapause in various insect species, however, little information is known about the detailed mechanisms. In this study, we investigated the roles of JH signaling in photoperiod reproductive diapause in a green lacewing, Chrysoperla nipponensis (Okamoto), which is a potentially important biological control predator. Our results showed that the short-day condition induces a diapause state including JH synthesis suppression, ovarian development arrest, and triglyceride accumulation. The interference of JH response genes, Krüppel homolog 1 (Kr-h1), in reproductive females exhibited a diapause-related phenotype such as ovarian development arrest and larger triglyceride storage. Exogenous JH III suppresses diapause to promote ovarian development and inhibit triglyceride synthesis. However, exogenous JH III fails to rescue the Kr-h1-silenced phenotype. Accordingly, our results demonstrate the critical role of Kr-h1 in regulating JH signaling to promote reproduction.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative resilience and precision of digitized optical counting using ImageJ during routine mosquito (Diptera: Culicidae) sample processing. 在常规蚊子（双翅目：Culicidae）样本处理过程中使用 ImageJ 进行数字化光学计数的复原力和精确度比较。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-03-14 DOI: 10.1093/jisesa/ieaf026

Ayla Faraji, Kelsey A Fairbanks, Ary Faraji, Christopher S Bibbs

{"title":"Comparative resilience and precision of digitized optical counting using ImageJ during routine mosquito (Diptera: Culicidae) sample processing.","authors":"Ayla Faraji, Kelsey A Fairbanks, Ary Faraji, Christopher S Bibbs","doi":"10.1093/jisesa/ieaf026","DOIUrl":"10.1093/jisesa/ieaf026","url":null,"abstract":"Surveillance is integral for the targeted and effective function of integrated vector management. However, the scale of surveillance efforts can be prohibitive on manpower, given the large number of traps set, collected, processed, and enumerated. For many public health agencies, the sheer effort of weekly trapping, combined with the processing of numerous traps, is a major capacity challenge. To reduce employee fatigue and increase throughput, estimation methods are used in a diagnostic capacity to determine threshold numbers of mosquitoes (Diptera: Culicidae) for operational decision-making. Historically, volume and mass measures correlated to a known number of mosquitoes are the oldest and most widely used within mosquito control programs. Image processing methods using digital counting software, such as ImageJ, have not been tested rigorously in the context of high throughput usage experienced in mosquito operations. We stress-tested volume, mass, and image processing methods using sample calibrations from early in the year and applied them throughout a mosquito active season. We additionally tested resilience with samples that had been frozen, desiccated, old, or from an excessively large trap collection. Furthermore, we compared magnitudes of error after intentionally deviating from best practices. In all cases, mass and volume encountered significant errors. In contrast, the digitized-optical counting method was resilient to going long periods of use without recalibrating, handling different species compositions, and processing aged or damaged samples. If a program has limited logistical power, the aforementioned image-processing method confers the best balance of accuracy and expediency for time-sensitive workloads and efficient operational decision making.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11908421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0