Journal of Insect Science最新文献

Characterization of the complete mitogenomes of two species of Eumolpinae (Coleoptera: Chrysomelidae) and phylogenetic insights. 两种金龟科（鞘翅目：金龟科）的全有丝分裂基因组特征及系统发育研究。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-05-05 DOI: 10.1093/jisesa/ieag037

Hathal M Al Dhafer, Mahmoud S Abdel-Dayem, Xiao-Ling Lin, Qi Fang, Ioannis Eleftherianos, Amr Mohamed, Rui-E Nie

{"title":"Characterization of the complete mitogenomes of two species of Eumolpinae (Coleoptera: Chrysomelidae) and phylogenetic insights.","authors":"Hathal M Al Dhafer, Mahmoud S Abdel-Dayem, Xiao-Ling Lin, Qi Fang, Ioannis Eleftherianos, Amr Mohamed, Rui-E Nie","doi":"10.1093/jisesa/ieag037","DOIUrl":"10.1093/jisesa/ieag037","url":null,"abstract":"The subfamily Eumolpinae holds substantial economic importance due to their plant-feeding habits. In this study, 2 complete mitochondrial genomes of eumolpine leaf beetles, Macrocoma budura Daccordi & Medvedev and Colasposoma grande grande (Lefèvre), were newly sequenced. Both mitogenomes possess the standard 37 mitochondrial genes and a large noncoding control region, and exhibit a highly conserved gene arrangement except for a diagnostic inversion of trnR (tRNA-Arg) and trnA (tRNA-Ala). The inversion of these 2 tRNAs has been reported previously in Eumolpinae and represents a potential subfamily-level synapomorphy. Most tRNAs adopt the typical cloverleaf conformation, with the exception of C. g. grande trnS1 (tRNA-Ser [AGN]), which lacks the DHU (dihydrouridine [tRNA structural domain]) arm, and the M. budura tRNA-Cys, which has a shortened D-loop, suggesting possible lineage-specific structural variation. Bayesian and maximum likelihood analyses recover C. g. grande within Euryopini, whereas M. budura is placed within Bromiini with an intratribal position that varies by dataset. These new mitogenomes not only contribute to the sparse genomic resources for Eumolpinae from arid regions but also demonstrate the value of mitochondrial gene order and sequence data to resolve species-level relationships and biogeographic histories within Chrysomelidae.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 3","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13143426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuticular microsculpturing: multispecies morphology of stridulatory structures in bark beetles of the genus Ips (Coleoptera: Curculionidae: Scolytinae). 表皮微雕刻：Ips属树皮甲虫（鞘翅目：曲蝇科：鞘翅科）的多物种壳状结构形态学。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag022

Ana Valeria Guzmán-Robles, Edwin R Ariza-Marín, Gerardo Cuéllar-Rodríguez, Francisco Armendáriz-Toledano

{"title":"Cuticular microsculpturing: multispecies morphology of stridulatory structures in bark beetles of the genus Ips (Coleoptera: Curculionidae: Scolytinae).","authors":"Ana Valeria Guzmán-Robles, Edwin R Ariza-Marín, Gerardo Cuéllar-Rodríguez, Francisco Armendáriz-Toledano","doi":"10.1093/jisesa/ieag022","DOIUrl":"10.1093/jisesa/ieag022","url":null,"abstract":"Stridulation is the only recognized mechanism for sound emission in Scolytinae, and at least half of its tribes manifest it. However, its morphology has been studied in almost 125 species, describing the location of the stridulatory apparatus. In a few groups, its morphological variation has been evaluated, mostly in characters of the striated surface of the pars stridens. Females of Ips DeGeer exhibit a stridulatory apparatus located on the pronotal apex and the vertex, which has been extensively documented in 18 of 37 species. However, the patterns of interspecific and intraspecific variation in pars stridens (on the vertex) and plectrum (on the pronotum) have not been thoroughly evaluated to test their taxonomic utility. Therefore, in this study, we proposed a set of 29 attributes for the pars stridens and plectrum to comprehensively quantify their morphological variation and evaluate their discriminatory power among eight species of Ips. These traits enable detailed characterization of the stridulatory apparatus, supporting their use as important diagnostic features. We also introduced a regionalization scheme for each structure to standardize comparisons across studies. Our results show that both structures covary, indicating phenotypic integration, and that the pars stridens offers stronger species-level discrimination than the plectrum.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12994702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147473832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of structured and seed-blend refuges for corn earworm (Lepidoptera: Noctuidae) using emergence cages and screen-bagged ear methods. 用应急笼和纱袋耳法评价玉米耳虫（鳞翅目：夜蛾科）的结构和混合种子避难所。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag030

Suhas S Vyavhare, G David Buntin, Joni L Blount, Matthew Carroll, Pat Porter

{"title":"Evaluation of structured and seed-blend refuges for corn earworm (Lepidoptera: Noctuidae) using emergence cages and screen-bagged ear methods.","authors":"Suhas S Vyavhare, G David Buntin, Joni L Blount, Matthew Carroll, Pat Porter","doi":"10.1093/jisesa/ieag030","DOIUrl":"10.1093/jisesa/ieag030","url":null,"abstract":"The corn earworm, Helicoverpa zea (Boddie), is a major lepidopteran pest of corn (Zea mays L.) in the United States and is widely managed using transgenic corn hybrids expressing Bacillus thuringiensis (Berliner) (Bt) proteins. Seed-blend refuges, in which Bt and non-Bt seeds are mixed in fixed proportions, have been widely adopted as a convenient insect resistance management (IRM) strategy. However, concerns remain about their efficiency in producing susceptible moths compared to a structured block refuge. To evaluate this, five field experiments were conducted across Texas and Georgia during 2020 to 2021 to compare adult H. zea emergence from non-Bt refuges configured as structured 100% non-Bt blocks or as 80:20 Bt/non-Bt seed blends. Adult production was quantified using two approaches: emergence cages that captured pupal-to-adult survival and screen bags that confined larvae on individual ears. Both methods yielded comparable estimates of adult H. zea emergence. Across all locations and years, seed-blend plots consistently produced fewer moths than expected based on the 20% refuge proportion, yielding 61% to 91% of the adults produced from structured refuges. Screen bag assays similarly showed reduced larval and pupal recovery from seed-blend ears. Non-Bt ears from seed-blend plots did not consistently differ from structured refuges in larval feeding intensity. Findings from this study indicate that seed-blend refuges may underperform in producing susceptible moths and may not consistently function as an effective standalone refuge strategy.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13050530/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating biocontrol potential of 6 parasitoid species (Hymenoptera) on apple and cherry aphids (Hemiptera: Aphididae) using no-choice bioassays. 采用无选择生物测定法评价6种膜翅目拟寄生蜂对苹果和樱桃蚜虫的防效。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag023

Rachele Quaglino, Nina Haas, Maiara Bastos Arteca, Leslie McCluckie, Barbara Egger

{"title":"Evaluating biocontrol potential of 6 parasitoid species (Hymenoptera) on apple and cherry aphids (Hemiptera: Aphididae) using no-choice bioassays.","authors":"Rachele Quaglino, Nina Haas, Maiara Bastos Arteca, Leslie McCluckie, Barbara Egger","doi":"10.1093/jisesa/ieag023","DOIUrl":"10.1093/jisesa/ieag023","url":null,"abstract":"European apple and cherry production depend on plant protection substances, but the variety of available options is continuously declining. Consequently, there is a need for alternative pest control strategies in orchards. An alternative method is the use of biocontrol agents, such as parasitoids. A range of parasitoid species is commercially available and a well-recognized strategy against aphid pests in protected crops. However, the efficacy of these species against apple and cherry aphid pests is still unexplored. Therefore, we conducted no-choice bioassays to assess the compatibility of 6 parasitoid species-Aphelinus abdominalis Dalman (Hymenoptera: Aphelinidae), Aphidius colemani Viereck (Hymenoptera: Braconidae), Aphidius ervi Haliday (Hymenoptera: Braconidae), Aphidius matricariae Haliday (Hymenoptera: Braconidae), Ephedrus cerasicola Stary (Hymenoptera: Braconidae), and Praon volucre Haliday (Hymenoptera: Braconidae)-against 3 target aphid species: Myzus cerasi Fabricius (Hemiptera: Aphididae), Aphis pomi De Geer (Hemiptera: Aphididae), and Dysaphis plantaginea Passerini (Hemiptera: Aphididae). The assays were conducted under greenhouse conditions on host tree cuttings (M. cerasi) or under controlled climate chamber conditions on saplings (A. pomi, D. plantaginea) with laboratory-reared aphid colonies. For each parasitoid species, the aphid colonies were inoculated with 5 to 10 females for a minimum of 72 h. The resulting parasitism rate was evaluated after 14 d. Our experiments show that host compatibility varies considerably between aphid species. Among the parasitoids tested, P. volucre and Aphi. matricariae emerged as the most consistent and effective species across all hosts. This suggests ecological compatibility and indicates that these 2 species are promising candidates for biological control in fruit orchards.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12989101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequence and structure analysis for chemosensory proteins and odorant-binding proteins in Nilaparvata lugens (Hemiptera: Delphacidae). 褐飞虱化学感觉蛋白和气味结合蛋白的序列与结构分析（半翅目：飞虱科）

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag025

Xiaoyi Dou, Russell Jurenka

{"title":"Sequence and structure analysis for chemosensory proteins and odorant-binding proteins in Nilaparvata lugens (Hemiptera: Delphacidae).","authors":"Xiaoyi Dou, Russell Jurenka","doi":"10.1093/jisesa/ieag025","DOIUrl":"10.1093/jisesa/ieag025","url":null,"abstract":"Chemosensation is crucial for insect behavior and physiology, involving several molecules. Chemosensory proteins (CSPs) and odorant-binding proteins (OBPs) transport volatile and lipophilic semiochemicals to activate the chemosensory system. Nilaparvata lugens Stål, is a monophagous pest of rice plants, causing significant economic losses worldwide. To date, 15 CSPs and 13 OBPs have been characterized, but their functions have not been fully investigated especially regarding their binding affinity with various ligands. This study compares the commonalities and differences between the 15 CSPs and 13 OBPs regarding sequence similarity, phylogeny, structural identity, and binding affinity with 3 different ligands. As expected, all 15 CSPs contain 4 conserved cysteine residues and 6 α-helical domains, whereas 13 OBPs have at least 6 cysteine residues and 6 α-helical domains. CSP1, 2, 8, and 14 cluster together in the phylogenetic tree, showing high structural similarity and similar binding affinity to the 3 ligands. OBP9 and 10 form a clade with high structural conservation; however, molecular docking indicates OBP9 exhibits a high binding affinity for the insecticide thiamethoxam. Three OBPs, OBP56d, OBPunknown1, and OBPunknown2 have been characterized and they display no significant preference for the 3 ligands. Overall, our integrated sequence and structural analysis reveals both functional redundancy and specific ligand preferences within the N. lugens chemosensory system. These findings provide a critical foundation for understanding the molecular basis of chemoreception in N. lugens and may inform the development of novel behavioral interference strategies.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13012876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host intestinal microbiota adaptive changes following Paranosema locustae infection and mechanism of chronic pathogenesis. 宿主肠道菌群的适应变化及其慢性发病机制。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag027

Huihui Zhang, Zhujun Cao, Xudong Zha, Weijing Wang, Roman Jashenko, Hongxia Hu, Rong Ji

{"title":"Host intestinal microbiota adaptive changes following Paranosema locustae infection and mechanism of chronic pathogenesis.","authors":"Huihui Zhang, Zhujun Cao, Xudong Zha, Weijing Wang, Roman Jashenko, Hongxia Hu, Rong Ji","doi":"10.1093/jisesa/ieag027","DOIUrl":"10.1093/jisesa/ieag027","url":null,"abstract":"Paranosema locustae infection reduces the abundance and diversity of the intestinal bacteria in locusts, although the microbial adaptive changes and the underlying mechanism of chronic pathogenesis remain unclear. In this study, the intestinal microbial changes in Calliptamus italicus (Linnaeus, 1758) (Orthoptera: Acrididae) were analyzed with metagenomic sequencing after P. locustae infection. Results showed that the diversity of intestinal microbial communities in C. italicus declined after P. locustae infection, while the abundance of infection-specific taxa in C. italicus in the experimental groups was significantly higher than those in the control groups, irrespective of sex (P<0.05). The populations of opportunistic pathogenic bacteria such as Klebsiella aerogenes and Enterococcus faecalis increased significantly (P < 0.05). Meanwhile, the abundances of probiotics such as Pediococcus acidilactici and Enterobacter hormaechei increased significantly (P <0.05), which could inhibit the pathogenicity of P. locustae. The results suggested that the interplay of changes in the species and quantities of probiotics and pathogenic bacteria in the intestine of C. italicus after P. locustae infection was an important factor contributing to the difficulty of P. locustae in quickly breaching the host defense system and to its chronic pathogenicity.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13035071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the host plant range of Contarinia nasturtii (Diptera: Cecidomyiidae), reveals novel hosts and lack of host plant resistance within a Brassica napus diversity panel. 研究了一种甘蓝型油菜（Brassica napus）的寄主植物范围，发现了新的寄主和缺乏抗性的寄主植物。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag009

Carina L Lopez, Juliana J Soroka, Sally L Vail, Meghan A Vankosky, Boyd A Mori

{"title":"Investigating the host plant range of Contarinia nasturtii (Diptera: Cecidomyiidae), reveals novel hosts and lack of host plant resistance within a Brassica napus diversity panel.","authors":"Carina L Lopez, Juliana J Soroka, Sally L Vail, Meghan A Vankosky, Boyd A Mori","doi":"10.1093/jisesa/ieag009","DOIUrl":"10.1093/jisesa/ieag009","url":null,"abstract":"Swede midge, Contarinia nasturtii Kieffer (Diptera: Cecidomyiidae), is an invasive pest of canola (Brassica napus Linnaeus, Brassica juncea Linnaeus, Brassica rapa Linnaeus) and other Brassicaceae crops that causes significant damage in eastern North America. Contarinia nasturtii has the potential to invade the Canadian Prairies, which represents North America's largest canola growing region. This study examined host plant range, female oviposition preference, and larval development of C. nasturtii on selected weed, cultivated, and model Brassicaceae species. We also examined potential host plant resistance using a diverse panel of B. napus lines and developed a novel measure of larval performance using the proportion of third instar larvae as a proxy for larval development. All tested weed species, except Descurainia sophia Linnaeus, supported C. nasturtii development and 5 new host plants (Lepidium densiflorum Schrad., Neslia paniculata (Linnaeus) Desv., Diplotaxis muralis (Linnaeus) DC., Camelina sativa (Linnaeus) Crantz, and Erysimum cheiranthoides Linnaeus) were identified. Notably, we provide the first evidence that Arabidopsis thaliana Linnaeus can be a host for C. nasturtii, establishing a novel model system for gall midge-plant interaction studies. Evaluation of B. napus lines found slight variation in oviposition but no strong resistance, suggesting the need to investigate resistance sources outside of B. napus. These findings expand our knowledge on the host range of C. nasturtii, introduce A. thaliana as a tractable experimental model system, and underscore the need for investigation of host plant resistance and the development of integrated pest management strategies for C. nasturtii to mitigate threats to North American canola production.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12962850/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sublethal effects of potato plant defenses induced via synthetic elicitors against the Colorado potato beetle (Coleoptera: Chrysomelidae). 合成激发子诱导马铃薯植物防御科罗拉多马铃薯甲虫的亚致死效应。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag013

Alexander M Butcher, Jana Lee, Silvia I Rondon

{"title":"Sublethal effects of potato plant defenses induced via synthetic elicitors against the Colorado potato beetle (Coleoptera: Chrysomelidae).","authors":"Alexander M Butcher, Jana Lee, Silvia I Rondon","doi":"10.1093/jisesa/ieag013","DOIUrl":"10.1093/jisesa/ieag013","url":null,"abstract":"Leptinotarsa decemlineata (Say) is among the most destructive insect pests of potato Solanum tuberosum L. worldwide. While conventional chemical tactics have repeatedly failed to control this pest due to the rapid development of resistance, elicitor-based enhancement of plant defenses using jasmonic acid (JA) or salicylic acid (SA) pathways offers a promising complementary approach. Hence, this study evaluated the effects of 3 commercially available plant defense elicitors: Blush 2X (JA type), Actigard 50WG (SA type), and Regalia (JA and SA type), on the growth, development, and performance of L. decemlineata larvae across 2 phenological stages of \"Russet Burbank\" potatoes, with and without Myzus persicae (Sulzer) co-infestation. Defoliation, larval development, weight, and excretion rates were assessed through a series of greenhouse bioassays. Blush 2X significantly reduced defoliation and delayed larval development, supporting the role of JA signaling in mediating antifeedant defenses. In contrast, high-rate Actigard 50WG increased defoliation and excretion while reducing larval weight, suggesting altered sugar allocation and a potential diuretic effect. Aphid presence induced signal interference, modifying elicitor efficacy in a plant stage-dependent manner. These findings highlight the potential of elicitor treatments to enhance integrated pest management strategies for L. decemlineata, while underscoring the importance of plant phenology, signal cross-talk, and ecological context in optimizing their deployment.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12978247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of worker traits and pollen provisions on bumble bee (Hymenoptera: Apidae) microcolony test performance. 工蜂性状和花粉供给对大黄蜂（膜翅目：蜂科）小群试验性能的影响。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag019

Ana R Cabrera, Daniel R Schmehl

{"title":"Influence of worker traits and pollen provisions on bumble bee (Hymenoptera: Apidae) microcolony test performance.","authors":"Ana R Cabrera, Daniel R Schmehl","doi":"10.1093/jisesa/ieag019","DOIUrl":"https://doi.org/10.1093/jisesa/ieag019","url":null,"abstract":"Bumble bee microcolonies have been used to investigate a range of research questions including pesticide effects. As a toxicity test, the microcolony can be a tool to observe concentration-based endpoints and has the flexibility for delivering a test substance via sucrose solution, pollen provision, or both. However, there is no regulatory guideline available nor an academic standard experimental design for bumble bee microcolonies and test performance can be influenced by pollen source, pollen amount, and worker selection. Our objectives were to evaluate the impact of worker size and age, as well as pollen quantity and quality, on microcolony test performance of common eastern bumble bee (Bombus impatiens Cresson). A cumulative 5 g of pollen provision during the first 10 d of colony establishment resulted in a higher number of emerged males at the end of the test compared to 2 g. The number of emerged males was different depending on the source and nutritional composition of the pollen. Mixed sizes and ages of workers yielded good test performance and are recommended based upon our findings. These collective results improve test performance (ie maximize the number of produced drones, maintain worker survival, and enable consumption of sucrose solution and pollen provisions, the latter as potential vehicles of test items in a toxicity test) when conducting a bumble bee microcolony study. These findings enhance the reliability of bumble bee microcolony studies and provide essential insights for developing standardized testing protocols that can better assess the impacts of environmental stressors on pollinator health.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12986759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147458186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of light levels and predator characteristics on the functional response of the European harvestman (Opiliones: Phalangiidae). 光照水平和捕食者特征对欧洲收割者功能反应的影响。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2026-03-02 DOI: 10.1093/jisesa/ieag021

Hannah E Stowe, Stella Uiterwaal

{"title":"Influence of light levels and predator characteristics on the functional response of the European harvestman (Opiliones: Phalangiidae).","authors":"Hannah E Stowe, Stella Uiterwaal","doi":"10.1093/jisesa/ieag021","DOIUrl":"10.1093/jisesa/ieag021","url":null,"abstract":"Subject Editor: Rachid HannaPredatory interactions form food webs and shape ecosystems. Such predator-prey relationships underlie the survival, fitness, and landscape utilization of predatory arthropods. Synanthropic arthropod species thrive in agroecosystems and in other landscapes transformed by human activity. Understanding the characteristics of synanthropic arthropod predator-prey relations is crucial to understanding and mitigating their impacts in nonnative ranges. One way to quantify predation is via the functional responses, which describe the strength and nature of predator-prey interactions. Predator traits and environmental conditions can influence functional responses, the resulting energy and nutrient flows, and ultimately population dynamics. Here, we present the first functional response quantification for the European harvestman (Phalangium opilio L.), an introduced opilionid species in the United States. We explore how predator sex, body size, leg characteristics, and environmental light levels influence foraging rates of wild-caught P. opilio on midge prey. We find that P. opilio exhibits a type II functional response on midges, and that predator sex, environmental light conditions, and many continuous predator characteristics such as body size and leg length do not influence functional response parameters. The number of legs missing also did not influence functional response parameters. Our results show that foraging rates in this species are primarily driven by prey density, while many classic determinants of interaction strengths do not appear to alter foraging. Our results improve our understanding of the role of nonnative predators in food webs and highlight the need for species-specific functional response experiments to quantify interaction strengths between novel predators and native prey.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"26 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13010330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147504040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0