Journal of Insect Science最新文献

Low genetic diversity of Aedes albopictus (Diptera: Culicidae) in El Salvador based on mitochondrial COI sequences: evidence of a well-preserved ancestral lineage in the Americas. 基于线粒体COI序列的萨尔瓦多白纹伊蚊（双翅目：库蚊科）低遗传多样性：美洲保存完好的祖先谱系的证据。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-07 DOI: 10.1093/jisesa/ieaf085

José L Palomo, Jonathan I Rogel, Miguel A Moreno, Andrea L Joyce

{"title":"Low genetic diversity of Aedes albopictus (Diptera: Culicidae) in El Salvador based on mitochondrial COI sequences: evidence of a well-preserved ancestral lineage in the Americas.","authors":"José L Palomo, Jonathan I Rogel, Miguel A Moreno, Andrea L Joyce","doi":"10.1093/jisesa/ieaf085","DOIUrl":"10.1093/jisesa/ieaf085","url":null,"abstract":"Aedes albopictus (Skuse) is an invasive mosquito species which has rapidly expanded across the Americas since the 1980s. This species has significant implications for public health. This study aimed to assess the genetic diversity and potential invasion routes of Ae. albopictus populations in El Salvador and throughout the Americas using the mitochondrial DNA (mtDNA) CO1 barcode. The mtDNA CO1 was sequenced from 52 samples from 5 departments in El Salvador. Analyses included the El Salvador populations, and an additional 205 GenBank sequences from the American continent and Asia, the region of origin. Haplotype and nucleotide diversity assessments, genetic differentiation, and phylogenetic clustering were performed. Genetic diversity in populations of Ae. albopictus from El Salvador and from most countries in America was generally low; El Salvador had 3 haplotypes. One exception was Colombia, where 12 haplotypes were detected. In the Americas, 22 haplotypes were found overall. Phylogenetic analyses grouped American samples into 2 major clusters, 1 centered around haplotype 19, which was the most abundant and widely distributed. El Salvador shared 2 haplotypes with North America. Ae. albopictus from Puerto Rico was genetically distinct from other groups and grouped together with Asian samples. The Asian outgroups were more diverse than samples from America. The analysis highlighted the presence of ancestral lineages in El Salvador and their role in early and complex colonization patterns of this species across the Americas, providing critical data for future management and control strategies against this invasive vector.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12501424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhythms of pheromone titer in 2 sugarcane pest species Chilo sacchariphagus and Chilo infuscatellus (Lepidoptera: Crambidae). 2种甘蔗害虫食糖螟和甘蔗螟信息素滴度的变化规律

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-07 DOI: 10.1093/jisesa/ieaf084

Liang He-Ling, Wei Ji-Li, Yu Yong-Hao, Zeng Xian-Ru, Mo Ren-Fu, Gao Yuan-Yuan, Zeng Xin-Nian, Liu Jia-Li, Long Xiu-Zhen

{"title":"Rhythms of pheromone titer in 2 sugarcane pest species Chilo sacchariphagus and Chilo infuscatellus (Lepidoptera: Crambidae).","authors":"Liang He-Ling, Wei Ji-Li, Yu Yong-Hao, Zeng Xian-Ru, Mo Ren-Fu, Gao Yuan-Yuan, Zeng Xin-Nian, Liu Jia-Li, Long Xiu-Zhen","doi":"10.1093/jisesa/ieaf084","DOIUrl":"10.1093/jisesa/ieaf084","url":null,"abstract":"Chilo sacchariphagus Bojer and Chilo infuscatellus Snellen are 2 major sugarcane pests in southern China. Both species are nocturnal moths that exhibit sexual activity during the night. However, the rhythms of their sexual activities remain poorly documented. In this study, we investigated the sex pheromone titers of both species to determine the effect of age on pheromone production and to characterize the rhythms of pheromone production during the scotophase, when the highest levels are expected to occur. Gas chromatography-mass spectrometry analysis of hexane extracts from female pheromone glands revealed that the titers of 3 sex pheromone components, (Z)-11-hexadecenyl acetate (Z11-16: Ac), (Z)-13-octadecenyl acetate (Z13-18: Ac), and (Z)-13-octadecen-1-ol (Z13-18: OH) were very low on the first night after eclosion, peaked on the second night, and then decreased with age, becoming nearly undetectable by the fourth night in C. sacchariphagus. A similar trend was observed in C. infuscatellus, in which the titer of (Z)-11-hexadecenol (Z11-16: OH) also peaked on the second scotophase. During the second scotophase, the titers of the 3 sex pheromone components in C. sacchariphagus increased rapidly over time from 21:00 to 05:00, peaking between 03:00 and 05:00, and then declined sharply. In contrast, the pheromone titer in C. infuscatellus remained stable during the first 4 h of the scotophase, then rose sharply between 01:00 and 03:00 before declining. These findings contribute to a better understanding of the sexual behavior of these 2 moths and provide a theoretical basis for precise monitoring strategies in the field.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12507019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sampling consistency matters-Stable isotopes (δ15N and δ13C) exhibit wide variation among individuals within bee species native to North America (Hymenoptera: Apidae). 取样一致性问题——稳定同位素（δ15N和δ13C）在北美原生蜜蜂（膜翅目：蜂科）个体之间表现出广泛的差异。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf083

Alexandra Tsalickis, Joshua W Campbell, Margaret Duffy, Richard S Vachula

{"title":"Sampling consistency matters-Stable isotopes (δ15N and δ13C) exhibit wide variation among individuals within bee species native to North America (Hymenoptera: Apidae).","authors":"Alexandra Tsalickis, Joshua W Campbell, Margaret Duffy, Richard S Vachula","doi":"10.1093/jisesa/ieaf083","DOIUrl":"10.1093/jisesa/ieaf083","url":null,"abstract":"Stable isotope analysis is scarcely utilized among bee researchers. One reason for this scarcity is ambiguity when determining whether to utilize the entire insect/arthropod or certain body parts for isotope analysis. Here, we analyzed δ15N and δ13C isotopic compositions of the abdomens, heads, and legs of 3 bee species native to North America [Centris pallida Fox, Melissodes bimaculatus (Lepeletier), and Bombus griseocollis (De Geer)]. We hypothesized that the abdomen δ15N and δ13C values would differ significantly from those of the heads and legs because the abdomen contains metabolically active organs, while the heads and legs, with less active tissues, would show no differences in isotopic compositions. Overall, we found varying degrees of statistical differences among the different body parts for isotope values. However, variations equivalent in magnitude to trophic level differences were not observed, resulting in the ability to use a single body part rather than a whole insect body for stable isotope analysis. To strengthen this finding, correlation analyses revealed paralleled patterns in responses by species. Finally, we suggest that when designing experiments using stable isotope analysis, it is important to focus on being consistent with selected body parts to reduce inter-individual variability. We also recommend using a relatively large sample size (n ≥ 20) due to the wide variance observed within individuals, especially if the goal is to characterize population-level ecological differences.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499767/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental validation of a rearing protocol for laboratory assays utilizing Osmia lignaria Say (Hymenoptera: Megachilidae). 实验验证了一种利用木浆木蚁（膜翅目：巨蜂科）进行实验室分析的饲养方案。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf081

Mary-Kate F Williams, Natalie K Boyle, Robert N Schaeffer, Diana L Cox-Foster

{"title":"Experimental validation of a rearing protocol for laboratory assays utilizing Osmia lignaria Say (Hymenoptera: Megachilidae).","authors":"Mary-Kate F Williams, Natalie K Boyle, Robert N Schaeffer, Diana L Cox-Foster","doi":"10.1093/jisesa/ieaf081","DOIUrl":"10.1093/jisesa/ieaf081","url":null,"abstract":"The blue orchard bee (Osmia lignaria Say) is a solitary bee native to North America that is increasingly propagated, sold, and used for pollination of rosaceous orchard crops. While methods exist to rear blue orchard bees in a laboratory setting, present protocols vary in diet manipulation and fail to progress bees to adult emergence. Variability in published methods also makes standardized comparisons within or across species challenging. Here, we present a validation of a rearing protocol for O. lignaria in a laboratory setting, with the study employed over a 2-year period that mirrored the bees' phenology in northern Utah (United States). Our protocol used 3D printed well plates with a well diameter that we recommend as appropriate for rearing O. lignaria in a laboratory. Each well permits the user to observe detailed life stages of individual O. lignaria bees without disturbing their development. To validate the protocol, we monitored O. lignaria development across 3 larval instars (first, second, and fifth), prepupal, pupal, and adult stages using a dissection scope and X-ray imaging. We confirm that diapause duration can be altered and affects the percent weight loss. Our data demonstrate that we can successfully rear bees to the adult stage (74%). Our protocol can be altered to fit any laboratory experiment and adapted to investigate other above-ground cavity-nesting bee species in a laboratory setting. Such investigations might include how multiple environmental conditions, nutritional factors, and stressors influence bee health.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wing melanization with 20-hydroxyecdysone modulation in Idea leuconoe clara (Lepidoptera: Nymphalidae). 20-羟基蜕皮素调控下的白蛉翅膀黑化。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf072

Ching-Lin Chu, Yi-Chen Chen, Tsen Hua, Ching-Ting Lai, Li-Hsin Wu

{"title":"The wing melanization with 20-hydroxyecdysone modulation in Idea leuconoe clara (Lepidoptera: Nymphalidae).","authors":"Ching-Lin Chu, Yi-Chen Chen, Tsen Hua, Ching-Ting Lai, Li-Hsin Wu","doi":"10.1093/jisesa/ieaf072","DOIUrl":"10.1093/jisesa/ieaf072","url":null,"abstract":"Temperature-induced changes in the physiological signals altered the wing coloration in Bicyclus anynana (Butler, 1879). The morphogenetic hormone 20-hydroxyecdysone (20E) increases with temperature and regulates the seasonal morph formation of the species. Although melanization in Idea leuconoe clara Butler (Lepidoptera: Nymphalidae) can be induced by temperature shock at 10, 35, and 40 °C, the relationship between this phenomenon and 20E remains unexplored. This study hypothesizes that the melanic phenotype of I. leuconoe clara is affected by distinct hormonal responses under extreme temperatures. The 20E levels were measured at the 3% and 20% pupal development stages of I. leuconoe clara under temperature shock. The relationship between 20E and melanization was investigated through hormone injection. The results demonstrated that the relationship between melanization under cold shock and 20E was negative and that 20E regulated the degree of melanin deposition rather than the patterning of melanized areas. This study investigates the effects 20E on wing melanization under extreme temperatures in I. leuconoe clara. Future studies could further determine the melanic changes in pigment and structural coloration through transcriptomic analysis and scanning electron microscopy based on the temperature shock procedure.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population genetics show that aphids (Hemiptera: Aphididae) are limited by summer host-plant distribution at the regional scale. 群体遗传学表明，在区域尺度上，蚜虫（半翅目：蚜虫科）受夏季寄主-植物分布的限制。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf082

Dion Garrett, Graham Teakle, Rosemary Collier, James R Bell, Ramiro Morales-Hojas

{"title":"Population genetics show that aphids (Hemiptera: Aphididae) are limited by summer host-plant distribution at the regional scale.","authors":"Dion Garrett, Graham Teakle, Rosemary Collier, James R Bell, Ramiro Morales-Hojas","doi":"10.1093/jisesa/ieaf082","DOIUrl":"10.1093/jisesa/ieaf082","url":null,"abstract":"Nasonovia ribisnigri (Mosley) is a severe aphid pest of outdoor lettuce, and the combination of sporadic and unpredictable colonization on outdoor lettuce, along with the breakdown of cultivar resistance, has left few effective control methods. The population structure (spatially and temporally) of N. ribisnigri is currently unknown in England, and therefore microsatellite markers were designed to estimate the impacts of host plant selection pressure (including host plant resistance) and environmental change. Biological samples collected between 2003 and 2020 from 10 sites across England were typed with microsatellite markers. The analysis of 8 microsatellites indicated a clear east-west divide between N. ribisnigri populations, which corresponds with current outdoor lettuce cultivation distribution in England, one of the aphid's summer hosts. Analysis of gene flow indicated that aphids did not leave the eastern region; instead, there was strong evidence for aphids migrating from the West into the secondary host eastern region, possibly from the winter host (Ribes spp.) in Spring. This result suggests that although N. ribisnigri has the potential for long-distance migration, strong ties to the summer host (lettuce) determine migratory behavior at the population level. N. ribisnigri are mostly holocyclic and show a high level of inbreeding. Long-term trends revealed relatively stable populations, despite a recent breakdown of host plant resistance and other environmental changes, including favorable temperatures. The geographic and temporal structure of the N. ribisnigri population is discussed in relation to future pest management strategies.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two hymenopteran parasitoid species of the jackfruit borer Diaphania caesalis (Lepidoptera: Pyralidae) in China. 标题中国菠萝蜜螟虫膜翅目二种寄生性昆虫（鳞翅目：蚜科）。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf079

Pei-Qiong Shi, Le Feng, Ke-Cong Huang, Jia-Cheng Hu, Feng Feng, Jin Xu

{"title":"Two hymenopteran parasitoid species of the jackfruit borer Diaphania caesalis (Lepidoptera: Pyralidae) in China.","authors":"Pei-Qiong Shi, Le Feng, Ke-Cong Huang, Jia-Cheng Hu, Feng Feng, Jin Xu","doi":"10.1093/jisesa/ieaf079","DOIUrl":"10.1093/jisesa/ieaf079","url":null,"abstract":"Jackfruit borer, Diaphania caesalis (Walker), is a major boring pest of Artocarpus plants (Moraceae). Biological control is considered an environmentally sustainable means of managing pests. However, parasitoids of D. caesalis in China are unknown. Here, we investigated the parasitoids of D. caesalis through field monitoring and surveys and identified them through morphological observation and DNA barcoding technology. Two hymenopteran parasitoid species, Dolichogenidea sp. and Eulophidae undet. sp., were identified on D. caesalis. The parasitism rate of Dolichogenidea sp. (21.0 ± 1.8%) was significantly higher than that of Eulophidae undet. sp. (3.8 ± 2.2%). The field incidence of Dolichogenidea sp. in the Artocarpus integer and Artocarpus heterophyllus orchards was 30.8 ± 2.5% and 22.3 ± 7.6%, respectively, but there was no significant difference. Overall, Dolichogenidea sp. was the dominant parasitoid of D. caesalis in China. Further research is needed to determine the species' identity, and their biological characteristics should be evaluated to determine their potential applications in biocontrol programs.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using harmonic radar to compare movement behavior between Bactrocera dorsalis and Ceratitis capitata (Diptera: Tephritidae). 用谐波雷达比较背小实蝇和头角certis（双翅目：绦虫科）的运动行为。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf078

Matthew S Siderhurst, Anika L Hurst, Nicolas Ladizinsky, Charlotte Aldebron

{"title":"Using harmonic radar to compare movement behavior between Bactrocera dorsalis and Ceratitis capitata (Diptera: Tephritidae).","authors":"Matthew S Siderhurst, Anika L Hurst, Nicolas Ladizinsky, Charlotte Aldebron","doi":"10.1093/jisesa/ieaf078","DOIUrl":"10.1093/jisesa/ieaf078","url":null,"abstract":"Behavioral differences between related insects may inform how pest management strategies or conservation efforts are tailored to specific species. Movement behavior is particularly important, but few studies have undertaken head-to-head comparisons to evaluate differences in interspecific movement. This study used harmonic radar to simultaneously track 2 agriculturally important tephritid fruit fly species, Bactrocera dorsalis (Hendel) and Ceratitis capitata (Wiedemann), in an outdoor cage (experiment 1) and a coffee field (experiments 2 and 3) to assess fly directional movement, distance, and speed parameters. In general, both fly species have similar movement parameters. However, experiment 2 showed B. dorsalis to be more active with fewer induced movements and less time needed to record the target number of movement steps. This was supported by a laboratory bioassay (experiment 4) that confirmed B. dorsalis were more active. Mean step-distances only differed in experiment 2 and were longer for C. capitata. Experiment 3 tracked tagged flies over time with 2 B. dorsalis located after 3 d post-release while no C. capitata were located after 48 h. Both species generally move with the prevailing wind. While this study found some differences in movement behaviors between these 2 fly pests, fly movement parameters were more similar than expected, suggesting that it may be possible to characterize the movement of key insect species and then generalize these findings to related taxa. If this is possible, control and detection strategies optimized with movement data from one species may be transferable to other pests, thereby increasing the impact of movement research.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499761/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dnmt1 is required for early embryo development in the haplodiploid insect, Bemisia tabaci (Hemiptera: Aleyrodidae). 单倍体烟粉虱（半翅目：粉虱科）的早期胚胎发育需要Dnmt1。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf077

Emily A Shelby, Elizabeth C McKinney, Christopher B Cunningham, Alvin M Simmons, Allen J Moore, Patricia J Moore

{"title":"Dnmt1 is required for early embryo development in the haplodiploid insect, Bemisia tabaci (Hemiptera: Aleyrodidae).","authors":"Emily A Shelby, Elizabeth C McKinney, Christopher B Cunningham, Alvin M Simmons, Allen J Moore, Patricia J Moore","doi":"10.1093/jisesa/ieaf077","DOIUrl":"10.1093/jisesa/ieaf077","url":null,"abstract":"The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a major economic pest that is difficult to manage with current strategies. New strategies will depend on better understanding the biology of whiteflies. For example, little is known about factors that affect primary sex ratio and embryological development in the haplodiploid system of B. tabaci, which may provide an entry point into new control strategies. In this study, we show that expression of DNA methyltransferase 1 (Dnmt1) is required for early embryogenesis in B. tabaci. First, we show that reduction of Dnmt1 expression using RNA interference decreased the number of eggs laid and the viability of eggs but did not affect the adult sex ratio. We also identify key developmental stages during embryogenesis, which have been shown to be consistent in both sexes. Embryos produced from dsDnmt1-treated females failed to form a germ rudiment and had smaller sized nuclei, suggesting inhibition of the cell cycle early in development. Although the specific mechanism by which DNMT1 affects embryogenesis remains elusive, that is, whether the effect is methylation dependent or independent, our study provides insights into DNMT1's function based on when and how DNMT1 is needed.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 5","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel method for quantifying galleries of bark beetles and associates. 一种量化树皮甲虫及其同伴画廊的新方法。

IF 2 3区农林科学

Journal of Insect Science Pub Date : 2025-10-06 DOI: 10.1093/jisesa/ieaf086

J Forest Palmer, Jess A Hartshorn

引用次数: 0