Journal of Insect Science最新文献_第2页

Investigating a role for piRNA-associated piwi genes in overcoming host-plant resistance in the soybean aphid, Aphis glycines. 研究pirna相关piwi基因在大豆蚜虫抗宿主植物抗性中的作用。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf055

Angel Haller, Jelmer W Poelstra, Wirat Pipatpongpinyo, Nathan Kreuter, Jennifer R Wilson, Andy Michel

{"title":"Investigating a role for piRNA-associated piwi genes in overcoming host-plant resistance in the soybean aphid, Aphis glycines.","authors":"Angel Haller, Jelmer W Poelstra, Wirat Pipatpongpinyo, Nathan Kreuter, Jennifer R Wilson, Andy Michel","doi":"10.1093/jisesa/ieaf055","DOIUrl":"10.1093/jisesa/ieaf055","url":null,"abstract":"Natural host-plant resistance provides a sustainable solution to control insect outbreaks but can be limited due to insect counter-adaptation. The exact mechanisms of insect adaptation to host-plant resistance remain unclear in most systems. Some insect adaptations are controlled by epigenetic mechanisms, such as through noncoding RNA. PIWI-interacting RNAs are specific noncoding RNAs that bind with PIWI proteins to control a diverse range of gene regulatory functions, particularly in insects. Previous investigation into aphid PIWI gene copies showed expansion in their abundance compared to other insects, which may suggest PIWI genes have additional functions among aphids. We first characterized PIWI gene evolution through a phylogenetic analysis, then investigated the role of PIWIs by examining gene expression in the soybean aphid (Aphis glycines), a significant insect pest of soybean which has adapted to overcome aphid-resistance in host plants. Our data indicated the presence of three PIWI ortholog groups, as well as taxon-specific gene expansions, with gene copy numbers ranging from 3 to 17 across species. To evaluate a potential role of PIWIs in overcoming host-plant resistance, we measured their gene expression in Ap. glycines with (virulent) and without (avirulent) the ability to survive on aphid-resistant soybean. We found that virulent Ap. glycines have significantly higher expression of 2 PIWI genes (Agl1.1 and Agl1.3) compared to the avirulent biotype. These data suggest that gene regulatory mechanisms related to the PIWI pathway, potentially including piRNAs, are important in aphid systems and may enable adaptation to host-plant resistance.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12144034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of candidate host-manipulating effector genes in Aphis gossypii (Hemiptera: Aphididae) using a combination of transcriptome, genome, and differential gene expression data. 利用转录组、基因组和差异基因表达数据的组合鉴定棉蚜（半翅目：蚜科）候选宿主操纵效应基因。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf053

Chaoyang Zhao, Nicholas Mueller, Isabella Owens, Raman Bansal, Alana L Jacobson

{"title":"Identification of candidate host-manipulating effector genes in Aphis gossypii (Hemiptera: Aphididae) using a combination of transcriptome, genome, and differential gene expression data.","authors":"Chaoyang Zhao, Nicholas Mueller, Isabella Owens, Raman Bansal, Alana L Jacobson","doi":"10.1093/jisesa/ieaf053","DOIUrl":"10.1093/jisesa/ieaf053","url":null,"abstract":"The cotton aphid, Aphis gossypii Glover, is an important plant disease vector and a highly polyphagous agricultural pest that feeds on a broad range of host plants. During feeding, its salivary glands serve as a route for the transmission of circulative plant viruses and produce a range of secretory proteins, called effectors, to modulate host cellular processes. To understand the molecular mechanisms underlying aphid-plant interactions, we developed a bioinformatics pipeline that incorporated the salivary gland transcriptome, genome, and head vs abdomen differential gene expression data to predict secretory protein-encoding genes enriched in the salivary glands of A. gossypii. Annotation of the 351 predicted genes showed that the most abundant functional categories were associated with cellular signaling and metabolism processes, and revealed that 98 genes were hemipteran-specific. Notably, 51 genes encode secretory proteins matching the putative saliva proteins identified in prior proteomics studies. Quantitative PCR analysis validated differential expression of 4 selected genes between heads and abdomens and indicated that alate adults exhibited the highest gene expression, suggesting these genes may play key roles in host colonization. Additionally, 25 genes showed sequence similarities to functionally characterized hemipteran effectors, with some appearing to form effector groups with distinct evolutionary patterns. Collectively, this study identified numerous putative plant-manipulating genes in A. gossypii and provided valuable insights into the mechanisms of aphid-plant interactions.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12118542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A non-destructive, fast, inexpensive, non-toxic chelating resin-based DNA extraction protocol for insect voucher specimens and associated microbiomes. 一种非破坏性，快速，廉价，无毒的螯合树脂为基础的昆虫凭证标本和相关微生物组的DNA提取方案。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf062

Morgan E Brown, Sara Ottati, Valeria Trivellone

{"title":"A non-destructive, fast, inexpensive, non-toxic chelating resin-based DNA extraction protocol for insect voucher specimens and associated microbiomes.","authors":"Morgan E Brown, Sara Ottati, Valeria Trivellone","doi":"10.1093/jisesa/ieaf062","DOIUrl":"10.1093/jisesa/ieaf062","url":null,"abstract":"Identifying a DNA extraction method that yields high quantity and quality DNA is a crucial component of molecular ecological studies; and the best suited method can vary greatly depending on research priorities. Here, we propose a nondestructive extraction method for insect museum vouchers aimed at analyzing gut-associated microbiomes. The leafhopper Euscelidius variegatus (Kirschbaum) (Hemiptera: Cicadellidae) associated with the bacterial plant pathogen Flavescence dorée phytoplasma, a member of the genus 'Candidatus Phytoplasma' (Mollicutes: Acholeplasmataceae), was used as an experimental model. We developed and refined a resin-based DNA extraction protocol by testing the effects of prelysis bleaching and postlysis proteinase K inactivation on DNA quality and yield. We found that bleaching did not compromise the integrity of insect and associated bacterial DNA and that excluding the inactivation of proteinase K did not interfere with quantitative polymerase chain reaction analysis. Based on our findings, we recommend a DNA extraction protocol for insect voucher specimens and associated microbiomes that includes a prelysis bleaching step to chemically degrade external contaminants without proteinase K inactivation, thereby reducing processing time. Our refined protocol resulted in a high DNA yield, which we successfully analyzed using quantitative polymerase chain reaction analysis and other downstream molecular applications, including targeted high-throughput sequencing.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12132034/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of s-methoprene-induced growth inhibition in Ephestia elutella (Hübner) (Lepidoptera: Pyralidae): insights from transcriptomic analysis. s-甲氧丁烯诱导的elutella （h<s:1> bner）生长抑制的分子机制（鳞翅目：Pyralidae）：转录组学分析的见解。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf035

Chao Huang, Jianhua Lü, Chunqi Bai, Yafei Guo, Chao Guo, Jizhen Song, Jiaqin Xi

{"title":"Molecular mechanisms of s-methoprene-induced growth inhibition in Ephestia elutella (Hübner) (Lepidoptera: Pyralidae): insights from transcriptomic analysis.","authors":"Chao Huang, Jianhua Lü, Chunqi Bai, Yafei Guo, Chao Guo, Jizhen Song, Jiaqin Xi","doi":"10.1093/jisesa/ieaf035","DOIUrl":"10.1093/jisesa/ieaf035","url":null,"abstract":"Ephestia elutella is a globally distributed storage pest, and its growth and development are regulated by juvenile hormones. To investigate the molecular mechanisms underlying the response of E. elutella larvae to the juvenile hormone analog s-methoprene, this study examined the effects of s-methoprene on the growth and development of E. elutella, explored the response of E. elutella to s-methoprene exposure by transcriptomic analysis, and confirmed its hub genes by RT-qPCR experiments. Larval mortality of E. elutella increased and adult emergence decreased with increasing exposure durations and doses of s-methoprene. After exposure at 5 × 10⁻⁵ mg/cm² of s-methoprene for 4 wk, a few of larvae pupated, but failed to emerge into adults, while at 50 × 10⁻⁵ mg/cm² for 4 wk, larvae were completely unable to pupate. Transcriptomic analysis identified 2,569 and 6,719 differentially expressed genes in the EE0 vs. EE5 and EE0 vs. EE50, respectively. Weighted Gene Co-expression Network Analysis identified 5 modules, with the yellow module most relevant to EE5. The genes in the yellow module were significantly enriched in biological processes. The Cluster-6182.18691, Cluster-6182.8343, Cluster-6182.28346, and Cluster-6182.21392 were hub genes in the yellow module. s-Methoprene directly or indirectly inhibited the growth and development of E. elutella larvae by affecting critical biological processes, such as hormonal regulation, etc. RT-qPCR validation confirmed the reliability of the transcriptomic data. This study provides important foundational data and theoretical insights into the molecular mechanisms of E. elutella in response to s-methoprene.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-model assessments to characterize occurrences of emerald ash borer (Coleoptera: Buprestidae). 多模型评估表征绿灰螟虫（鞘翅目：布氏科）的发生。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf032

Kishan R Sambaraju, Kathryn A Powell, André Beaudoin

{"title":"Multi-model assessments to characterize occurrences of emerald ash borer (Coleoptera: Buprestidae).","authors":"Kishan R Sambaraju, Kathryn A Powell, André Beaudoin","doi":"10.1093/jisesa/ieaf032","DOIUrl":"10.1093/jisesa/ieaf032","url":null,"abstract":"Introduction and spread of nonindigenous species present a formidable threat to forest health. The emerald ash borer (EAB), Agrilus planipennis, is an East Asian-origin insect that has devastated ash (Fraxinus spp.) trees across the United States and parts of Canada since 2002. Proactive surveillance using high-performing predictive models could aid in mitigating pest risk. Predictor variables and modeling methods are important considerations in such analysis. Therefore, we assessed whether relevant single predictors, a combination of predictors grouped under a certain driver category, or multiple key predictors comprising several drivers, alter the goodness-of-fit of logistic regression models to EAB occurrence data (2002 to 2018) from Canada. The predictors used in models included spatial, topographic/positional, transport pathways/human hotspots, host-related factors, and climate-related variables. Using predictors from the best candidate logistic regression model, we tested the performance of 7 different model types including an ensemble model. Our findings showed that predictors from a wide range of drivers better characterized EAB occurrences than single predictors or a combination of predictors from any given driver category. In multi-model comparisons, random forest outperformed all other models, including the ensemble model. Elevation, infestation pressure, accumulated degree-days (>10 °C), and human population density were important predictors of EAB presence. Random forest and ensemble model forecasts for the city of Edmonton, Alberta, Canada, indicated an area of potential concern for EAB. Our research strongly underscores the utility of comparative multi-model approaches in invasive risk assessments that could have important implications for pest surveillance and management.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12132038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Responses of house crickets (Orthoptera: Gryllidae) to various dietary gross energy levels: effects on growth performance and nutrient deposition. 家蟋蟀（直翅目：灰蟋蟀科）对不同饲粮总能水平的反应：对生长性能和养分沉积的影响。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf059

Sutisa Khempaka, Supattra Okrathok, Jan Th Schonewille, Chayanan Pukkung, Merisa Sirisopapong, Orapin Jantasaeng, Phocharapon Pasri

{"title":"Responses of house crickets (Orthoptera: Gryllidae) to various dietary gross energy levels: effects on growth performance and nutrient deposition.","authors":"Sutisa Khempaka, Supattra Okrathok, Jan Th Schonewille, Chayanan Pukkung, Merisa Sirisopapong, Orapin Jantasaeng, Phocharapon Pasri","doi":"10.1093/jisesa/ieaf059","DOIUrl":"10.1093/jisesa/ieaf059","url":null,"abstract":"Crickets present a sustainable protein alternative with a lower ecological footprint compared to traditional animal proteins. This research aimed to investigate the effect of dietary energy density on feed intake, growth, and body composition in house crickets (Acheta domesticus L., Orthoptera: Gryllidae) up to 45 d after hatching. The study consisted of 2 phases (7 to 20 and 21 to 45 d of age), with house crickets randomly assigned to 5 dietary treatments, each with six replicates. Dietary energy levels ranged from 3,819 to 4,265 kcal gross energy (GE)/kg in phase 1 and from 3,978 to 4,405 kcal GE/kg in phase 2. As dietary energy density increased, feed intake linearly decreased, while body mass linearly increased. In phase 1, protein retention increased from 72.1% to 85.5% as GE increased from 3,819 to 4,265 kcal /kg. Similarly, in phase 2, protein retention increased from 53.3% to 59.3% as GE increased from 3,978 to 4,379 kcal/kg. Correspondingly, the feed conversion ratio (FCR) improved with increasing dietary GE values. Broken-line analysis revealed the lowest FCR at 4,158 and 4,382 kcal GE/kg feed for house crickets from 7 to 20 and 21 to 45 d after hatching, respectively. These findings confirm the relevance of energy density in achieving optimal growth performance and provide valuable insights for formulating nutritious cricket diets. However, caution is warranted when extrapolating these results, as diets were formulated using GE instead of metabolizable energy (ME). Future studies should determine cricket-specific ME values to fine-tune dietary energy density.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168125/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Bactrocera minax (Diptera: Tephritidae) adult population density on its marginal distribution. 小小实蝇成虫密度对其边缘分布的影响。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf028

Yibing Guo, Bo Xu, Cong Huang, Huimin Yang, Fulian Wang, Lianyou Gui, Guifen Zhang

{"title":"Effect of Bactrocera minax (Diptera: Tephritidae) adult population density on its marginal distribution.","authors":"Yibing Guo, Bo Xu, Cong Huang, Huimin Yang, Fulian Wang, Lianyou Gui, Guifen Zhang","doi":"10.1093/jisesa/ieaf028","DOIUrl":"10.1093/jisesa/ieaf028","url":null,"abstract":"In order to explore the impact of insect density on the edge effect of Bactrocera minax (Diptera: Tephritidae) adult distribution in orange orchards, traps were set up in orchards with maggot infestation rates of 2%, 4%, and 20% to attract adults. The study compared differences in distribution between the side with noncitrus trees and the side farther away from them. The results showed that at lower insect densities (2% and 4% maggot infestation rates), the proportion of insect trap sites and the number of insects per trap on the side of the orchard adjacent to the trees were significantly higher than that on the side away from the noncitrus trees, additionally, the proportion of adults captured 10 to 20 m away from the side of noncitrus trees was also significantly higher than at other distances. However, at higher insect density (20% maggot infestation rates), there were no significant differences in the proportion of insect traps or the number of insects per trap between the adjacent and distant sides of the trees. Similarly, there were no significant differences in the proportion of adults captured at distances of 10, 20, 30, and 40 m away from the side of noncitrus trees in the orchard. In summary, at low insect density, B. minax adults exhibit a strong edge effect, concentrating on the side of the orchard with noncitrus trees, whereas at high insect density, they are evenly distributed throughout the orchard.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seasonal variation in floral resource use by a hover fly (Melangyna) (Diptera: Syrphidae) and the western honey bee (Apis mellifera) (Hymenoptera: Apidae) in an urban setting. 城市环境中飞蝇（双翅目：蚜科）和蜜蜂（膜翅目：蚜科）利用花卉资源的季节变化。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf052

Manuel E Lequerica Támara, Tanya Latty, Caragh G Threlfall, Dieter F Hochuli

{"title":"Seasonal variation in floral resource use by a hover fly (Melangyna) (Diptera: Syrphidae) and the western honey bee (Apis mellifera) (Hymenoptera: Apidae) in an urban setting.","authors":"Manuel E Lequerica Támara, Tanya Latty, Caragh G Threlfall, Dieter F Hochuli","doi":"10.1093/jisesa/ieaf052","DOIUrl":"10.1093/jisesa/ieaf052","url":null,"abstract":"Concerns about the impacts of introduced honey bees (Apis mellifera L.) on native insects and pollination services are prevalent. These impacts may be more pronounced on urban greenspaces, yet research on the use of abundant winter-flowering plants, such as Acacia spp. and Grevillea spp., in subtropical ecosystems is limited. Our study investigated the seasonal use of floral resources by honey bees and native hover flies (Melangyna indet.) in urban greenspaces of varying urbanization levels in Sydney, Australia. We found both insect groups use similar plants, with honey bees having a higher number of interactions with plants in general, but Melangyna interacting with more plant species during some seasons. Particularly in autumn and winter, when native plants flower, Melangyna had more interactions and visited more plant species than honey bees. The overlap in floral resources used by both was higher than expected in various urbanization levels during spring and autumn. This suggests that Melangyna may play a significant role in pollination during autumn and winter, especially in highly urbanized sites. The observed differences in floral resource utilization and seasonal variations indicate that the potential effects of introduced A. mellifera on native pollinators like Melangyna may be difficult to quantify given the high number of variables and complexity of the system. Our findings underscore the importance of considering seasonal patterns and multispecies interactions in studies on the impact of introduced species on native biodiversity.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12132036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longevity, feeding behavior, and egg production of adult house flies (Diptera: Muscidae) provided with potential foods on dairy farms. 为奶牛场提供潜在食物的成年家蝇（双翅目：蝇科）的寿命、摄食行为和产卵量。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf045

Anna E Grimenstein, Melissa A Doyle, Bethia H King, Chris J Geden

{"title":"Longevity, feeding behavior, and egg production of adult house flies (Diptera: Muscidae) provided with potential foods on dairy farms.","authors":"Anna E Grimenstein, Melissa A Doyle, Bethia H King, Chris J Geden","doi":"10.1093/jisesa/ieaf045","DOIUrl":"https://doi.org/10.1093/jisesa/ieaf045","url":null,"abstract":"Adult house flies (Musca domestica L.) are often a major pest at livestock facilities, where oviposition occurs on decomposing organic matter, such as manure. Some potential foods that adult house flies might consume on dairy farms were examined. Relative to when they were given water alone, survival of males and females was greater when they were given water along with liquid whole milk, formulated calf feed, or corn silage, or finely milled sorghum or soy, or buckwheat inflorescences. However, survival was significantly lower with these foods than with sucrose, although not significantly so for males with milk. There was little to no survival advantage when flies were given water along with milled hominy, wet spent brewers grain, or manure than with water alone. Both males and females spent significant time with their labellum in contact with corn silage, dandelion inflorescences, and buckwheat inflorescences, but little time with their labellum contacting manure or white clover inflorescences. Egg production was not increased by access to water along with buckwheat inflorescences or corn silage relative to sucrose; but egg production was increased by access to liquid whole milk. Reaching mature vitellogenesis stages was improved by prior exposure to water and sucrose solution along with a mixture of dry sucrose, milk, and yolk, or along with calf manure or formulated calf feed, but not with milled soy, sorghum, or hominy, or with wet spent brewers grain or citrus pellets. The diet including sucrose-milk-yolk resulted in the most females reaching mature vitellogenesis stages.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12062576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness and the synergism effects of Ipomoea cairica leaf plant extract and Metarhizium anisopliae fungi (Meta-G4) against larvae of Aedes aegypti Linnaeus and Aedes albopictus Skuse (Diptera: Culicidae). 金龟子叶提取物与金龟子绿僵菌真菌（Meta-G4）对埃及伊蚊和白纹伊蚊幼虫的防效及增效作用

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2025-05-09 DOI: 10.1093/jisesa/ieaf044

Kin Hoong Ng, Wan Fatma Zuharah

{"title":"Effectiveness and the synergism effects of Ipomoea cairica leaf plant extract and Metarhizium anisopliae fungi (Meta-G4) against larvae of Aedes aegypti Linnaeus and Aedes albopictus Skuse (Diptera: Culicidae).","authors":"Kin Hoong Ng, Wan Fatma Zuharah","doi":"10.1093/jisesa/ieaf044","DOIUrl":"10.1093/jisesa/ieaf044","url":null,"abstract":"Insecticide resistance is a major public health concern. Biopesticides, derived from naturally occurring substances such as plant extracts and fungal metabolites, are utilized as natural control agents against mosquito vectors. This study focuses on biopesticides to reduce chemical insecticide use in Penang Island, Malaysia. Ipomoea cairica Linnaeus Sweet (Solanales: Convolvulaceae) leaf extracts, Metarhizium anisopliae s.l. (Metsch) Sorok (Ascomycota: Clavicipitaceae) strain Meta-G4, and synergistic effects of both agents were demonstrated against Aedes albopictus Skuse (Diptera: Culicidae) and Aedes aegypti Linnaeus (Diptera: Culicidae) using larvicidal bioassays. Before assessing synergism, the compatibility of both agents was performed to minimize inhibition of mycelial growth on potato dextrose agar. The results showed that Ae. aegypti field strains (urban and suburban) are significantly more susceptible to I. cairica leaf extracts compared to Ae. albopictus field strains (urban and suburban) due to the lower lethal concentrations (LC50 and LC95). Aedes albopictus suburban field strain is significantly more susceptible than other strains when tested with M. anisopliae due to the lower LC50. The combination of M. anisopliae (1 × 106 conidia/ml) with I. cairica leaf extracts (350 ppm) achieved the lowest LT50 and LT95 against Ae. aegypti urban field strain. This study indicates that the synergistic combination of both agents exhibits significant larvicidal efficacy and holds promise for future biological control strategies targeting Aedes populations.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"25 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12070480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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