Journal of Insect Science最新文献_第3页

Molecular identification of predation on the Dubas bug (Hemiptera: Tropiduchidae) in Oman date palms: density-dependent response to prey. 阿曼枣椰树杜巴斯蝽（半翅目：杜巴斯蝽科）捕食的分子鉴定：对猎物的密度依赖性反应。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae088

Kacie J Athey, Eric G Chapman, Salem Al-Khatri, Abdel Moneim Moktar, John J Obrycki

{"title":"Molecular identification of predation on the Dubas bug (Hemiptera: Tropiduchidae) in Oman date palms: density-dependent response to prey.","authors":"Kacie J Athey, Eric G Chapman, Salem Al-Khatri, Abdel Moneim Moktar, John J Obrycki","doi":"10.1093/jisesa/ieae088","DOIUrl":"10.1093/jisesa/ieae088","url":null,"abstract":"The date palm (Phoenix dactylifera L.) (Arecales: Arecaceae) is the most economically important crop in Oman with an annual production of >360,000 tons of fruit. The Dubas bug (Ommatissus lybicus de Bergevin) (Hemiptera: Tropiduchidae) is one of the major pests of date palms, causing up to a 50% reduction in fruit production. Across the course of 2 seasons, a variety of arthropod predators living in the date palm canopy were investigated for possible biological control of Dubas bugs, given the growing interest in nonchemical insect pest control in integrated pest management. We collected ~6,900 arthropod predators directly from date palm fronds from 60 Omani date palm plantations and tested them for Dubas bug predation using PCR-based molecular gut content analysis. We determined that ≥56 species of arthropod predators feed on the Dubas bug. We found that predatory mites, ants, and the entire predator community combined showed a positive correlation between predation detection frequency and increasing Dubas bug density. Additionally, there was a significant impact of season on gut content positives, with the spring season having a significantly higher percentage of predators testing positive for Dubas bug, suggesting this season could be the most successful time to target conservation biological control programs utilizing a diverse suite of predators.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oven-drying and decontamination effects on crude protein concentration and in vitro crude protein digestibility of yellow mealworm (Tenebrio molitor) (Coleoptera: Tenebrionidae). 烘干和去污对黄粉虫（鞘翅目：黄粉虫科）粗蛋白浓度和体外粗蛋白消化率的影响

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae078

Giovanna Gómez-Oquendo, Andrés Loza Puerta, Cesar Gonzales Gutierrez, Carlos A Gómez-Bravo, Khaterine Salazar-Cubillas

{"title":"Oven-drying and decontamination effects on crude protein concentration and in vitro crude protein digestibility of yellow mealworm (Tenebrio molitor) (Coleoptera: Tenebrionidae).","authors":"Giovanna Gómez-Oquendo, Andrés Loza Puerta, Cesar Gonzales Gutierrez, Carlos A Gómez-Bravo, Khaterine Salazar-Cubillas","doi":"10.1093/jisesa/ieae078","DOIUrl":"10.1093/jisesa/ieae078","url":null,"abstract":"The study aims to assess the impact of oven-drying and decontamination on crude protein concentration and in vitro crude protein digestibility of yellow mealworms. Two kilograms of 12-wk-old mealworm larvae were subjected to freezing prior to the drying process. Approximately 1.5 kg of mealworm larvae were divided into 3 groups and exposed to oven-drying at temperatures of 50 °C for 36 h, 60 °C, and 70 °C for 24 h each. At intervals of 2 h, sets of 3 replicates were withdrawn to record water loss. Consistent weight stabilization was observed at 36 h for 50 °C (T50), 18 h for 60 °C (T60), and 14 h for 70 °C (T70). The remaining 0.5 kg of mealworm larvae was divided and dried under treatments T50, T60, and T70. Each treatment was then split into 2 portions, with one portion subjected to 90 °C for 15 min (denoted as T50-90, T60-90, T70-90) to eliminate microbial contamination. The 6 treatments were then used to determine concentrations of dry matter, crude ash, crude protein, pre-caecal protein digestibility, and dry matter residues after neutral detergent fiber, acid detergent fiber, and acid detergent lignin treatments. No interaction was observed between drying and decontamination treatments (P > 0.17). Pre-caecal crude protein digestibility increased with decreasing temperature (T50: 58% crude protein; T60: 51% crude protein; T70: 50% crude protein). Therefore, lower temperatures for longer times preserve crude protein digestibility. These findings are crucial for understanding how drying temperature and time impact protein bioavailability.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11310299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141906732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generating and testing the efficacy of reagents for CRISPR/Cas9 homology directed repair-based manipulations in Tribolium. 生成并测试试剂的功效，以便在蒺藜中进行基于 CRISPR/Cas9 同源定向修复的操作。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae082

Hannah C Markley, Kennedy J Helms, Megan Maar, Gabriel E Zentner, Michael J Wade, Andrew C Zelhof

{"title":"Generating and testing the efficacy of reagents for CRISPR/Cas9 homology directed repair-based manipulations in Tribolium.","authors":"Hannah C Markley, Kennedy J Helms, Megan Maar, Gabriel E Zentner, Michael J Wade, Andrew C Zelhof","doi":"10.1093/jisesa/ieae082","DOIUrl":"10.1093/jisesa/ieae082","url":null,"abstract":"CRISPR/Cas9 manipulations are possible in many insects and ever expanding. Nonetheless, success in one species and techniques developed for it are not necessarily applicable to other species. As such, the development and expansion of CRISPR-based (clustered regularly interspaced short palindromic repeats) genome-editing tools and methodologies are dependent upon direct experimentation. One useful technique is Cas9-dependent homologous recombination, which is a critical tool for studying gene function but also for developing pest related applications like gene drive. Here, we report our attempts to induce Cas9 homology directed repair (HDR) and subsequent gene drive in Tribolium castaneum (Herbst; Insecta: Coleoptera: Tenebrionidae). Utilizing constructs containing 1 or 2 target gRNAs in combination with Cas9 under 2 different promoters and corresponding homology arms, we found a high incidence of CRISPR/Cas9 induced mutations but no evidence of homologous recombination. Even though the generated constructs provide new resources for CRISPR/Cas9 modification of the Tribolium genome, our results suggest that additional modifications and increased sample sizes will be necessary to increase the potential and detection for HDR of the Tribolium genome.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11333919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mountain pine beetle (Coleoptera: Curculionidae) adult development rate model confirms evolved geographic differences. 山松甲虫（鞘翅目：Curculionidae）成虫发育率模型证实了进化的地理差异。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae074

Catherine E Wangen, James A Powell, Barbara J Bentz

{"title":"A mountain pine beetle (Coleoptera: Curculionidae) adult development rate model confirms evolved geographic differences.","authors":"Catherine E Wangen, James A Powell, Barbara J Bentz","doi":"10.1093/jisesa/ieae074","DOIUrl":"10.1093/jisesa/ieae074","url":null,"abstract":"Insects live in a wide range of thermal environments and have evolved species- and location-specific physiological processes for survival in hot and cold extremes. Thermally driven dormancy strategies, development rates and thresholds are important for synchronizing cohorts within a population and to local climates and often vary among populations within a species. Mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae, Scolytinae), is a widely distributed forest insect native to North America with clinal genetic differentiation in thermally dependent traits. MPB development occurs in Pinus phloem beneath the bark, and its cryptic habitat makes experimentation difficult, particularly for the adult stage. We describe a novel method for modeling MPB adult development following pupation and terminating in emergence from a brood tree. We focus on an Arizona (southern) MPB population with previously described preadult development rates. Field-observed tree attack, adult emergence, and phloem temperature data are combined in a parameterized cohort model and candidate rate curves are evaluated to describe adult emergence timing. Model competition indicates that the Brière rate curve provided the best fit to field data and performed well under cross-validation. Results confirm that the development of Arizona MPB adults is slower than the previously described development rate of more northern Utah adults. Using the estimated adult rate curve in a scenario of increasing mean temperatures, we show that the timing of second-generation adult emergence in the same year would result in cold-intolerant lifestages during winter, limiting the success of bivoltine populations.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11520411/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential insect threats to pennycress, Thlaspi arvense (Brassicales: Brassicaceae), an emerging oilseed cover crop. 昆虫对新兴油籽覆盖作物菥蓂（Thlaspi arvense，十字花科）的潜在威胁。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae086

Ellen O Adjeiwaa, Arthur V Ribeiro, Robert L Koch

{"title":"Potential insect threats to pennycress, Thlaspi arvense (Brassicales: Brassicaceae), an emerging oilseed cover crop.","authors":"Ellen O Adjeiwaa, Arthur V Ribeiro, Robert L Koch","doi":"10.1093/jisesa/ieae086","DOIUrl":"10.1093/jisesa/ieae086","url":null,"abstract":"Pennycress (Thlaspi arvense L.) is an annual plant in temperate regions that often grows as a weed. Pennycress is being domesticated as a new winter cover crop and oilseed crop for incorporation in the Midwest United States corn-soybean rotation, where it could offer economic and environmental benefits. While pennycress is gaining attention as a promising new crop, there remains a significant gap in understanding its interaction with insect communities and agroecosystems. This review compiles available information on insect herbivores (potential pests) and beneficial insects associated with pennycress growing in the wild (natural areas) or as a weed in agricultural areas. The limited knowledge on the response of pennycress to stressors (defoliation, stem injury and stand loss) similar to injury that could be caused by insects is also compiled here. By shedding light on the insects associated with pennycress and how pennycress might respond to injury from insect pests, this review sets the stage for further research and development of integrated pest management programs for insect pests of this new crop.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11347772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogeny, biogeography, and host range of gall midges (Diptera: Cecidomyiidae) feeding on spores of rust fungi (Basidiomycota: Pucciniales). 以锈菌孢子为食的五倍子蠓（双翅目：Cecidomyiidae）的系统发育、生物地理学和寄主范围（担子菌纲：Pucciniales）。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae077

Paula Andrea Gómez-Zapata, Melissa A Johnson, Teresa Bonacci, M Catherine Aime

{"title":"Phylogeny, biogeography, and host range of gall midges (Diptera: Cecidomyiidae) feeding on spores of rust fungi (Basidiomycota: Pucciniales).","authors":"Paula Andrea Gómez-Zapata, Melissa A Johnson, Teresa Bonacci, M Catherine Aime","doi":"10.1093/jisesa/ieae077","DOIUrl":"10.1093/jisesa/ieae077","url":null,"abstract":"Rust fungi (Pucciniales) are plant pathogens that can cause devastating yield losses to economically important crops and threaten native plants with extinction. Rusts are usually controlled with fungicides when rust-resistant plant varieties are unavailable. However, natural enemies may offer an alternative to chemicals by acting as biological controls. The larvae of Mycodiplosis Rübsaamen (49 spp.) feed on the spores of rusts and powdery mildew fungi and have been suggested as a potential biocontrol candidate for disease-causing rusts. However, little is known about the phylogenetic relationships, biogeography, and host range of this genus. We screened 5,665 rust specimens from fungarium specimens and field collections and recovered a total of 363 larvae on 315 rust specimens from 17 countries. Three mitochondrial and 2 nuclear loci were amplified and sequenced for the phylogenetic reconstruction of 129 individuals. We recovered 12 clades, of which 12 and 10 were supported with maximum likelihood and Bayesian inference, respectively. Of the 12 clades, 7 comprised species from multiple continents and climatic regions, and 5 comprised species from a single region. Individuals forming clades were collected from 2 to 18 rust species, suggesting that Mycodiplosis species have a broad host range. In total, Mycodiplosis larvae were identified on 44 different rust species collected from 18 plant families. Future studies should focus on expanding field sampling efforts, including data from additional gene regions, and incorporating morphological data to further elucidate species diversity and distribution patterns.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11350377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A molecular approach to unravel trophic interactions between parasitoids and hyperparasitoids associated with pecan aphids. 用分子方法揭示与山核桃蚜虫有关的寄生虫和超寄生虫之间的营养相互作用。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae071

Eddie K Slusher, Ted Cottrell, Tara Gariepy, Angelita Acebes-Doria, Marina Querejeta Coma, Pedro F S Toledo, Jason M Schmidt

{"title":"A molecular approach to unravel trophic interactions between parasitoids and hyperparasitoids associated with pecan aphids.","authors":"Eddie K Slusher, Ted Cottrell, Tara Gariepy, Angelita Acebes-Doria, Marina Querejeta Coma, Pedro F S Toledo, Jason M Schmidt","doi":"10.1093/jisesa/ieae071","DOIUrl":"10.1093/jisesa/ieae071","url":null,"abstract":"Advances in molecular ecology can overcome many challenges in understanding host-parasitoid interactions. Genetic characterization of the key-players in systems helps to confirm species and identify trophic linkages essential for ecological service delivery by biological control agents; however, relatively few agroecosystems have been explored using this approach. Pecan production consists of a large tree perennial system containing an assortment of seasonal pests and natural enemies. As a first step to characterizing host-parasitoid associations in pecan food webs, we focus on aphid species and their parasitoids. Based on DNA barcoding of field-collected and reared specimens, we confirmed the presence of 3 species of aphid, one family of primary parasitoids, and 5 species of hyperparasitoids. By applying metabarcoding to field-collected aphid mummies, we were able to identify multiple species within each aphid mummy to unravel a complex food web of 3 aphids, 2 primary parasitoids, and upward of 8 hyperparasitoid species. The results of this study demonstrate that multiple hyperparasitoid species attack a single primary parasitoid of pecan aphids, which may have negative consequences for successful aphid biological control. Although further research is needed on a broader spatial scale, our results suggest multiple species exist in this system and may suggest a complex set of interactions between parasitoids, hyperparasitoids, and the 3 aphid species. This was the first time that many of these species have been characterized and demonstrates the application of novel approaches to analyze the aphid-parasitoid food webs in pecans and other tree crop systems.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Do Varroa destructor (Acari: Varroidae) mite flows between Apis mellifera (Hymenoptera: Apidae) colonies bias colony infestation evaluation for resistance selection? 破坏螨（Acari: Varroidae）在蜂群之间的流动是否会影响抗性选择的蜂群侵染评估？

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae068

Matthieu Guichard, Adrien von Virag, Benoît Droz, Benjamin Dainat

{"title":"Do Varroa destructor (Acari: Varroidae) mite flows between Apis mellifera (Hymenoptera: Apidae) colonies bias colony infestation evaluation for resistance selection?","authors":"Matthieu Guichard, Adrien von Virag, Benoît Droz, Benjamin Dainat","doi":"10.1093/jisesa/ieae068","DOIUrl":"10.1093/jisesa/ieae068","url":null,"abstract":"Since the global invasion of the ectoparasitic mite Varroa destructor (Anderson and Trueman), selection of mite-resistant honey bee (Apis mellifera L.) colonies appears challenging and has to date not broadly reduced colony mortality. The low published estimated heritability values for mite infestation levels could explain the limited genetic progresses obtained so far. We hypothesize that intercolonial horizontal mite transmission could differentially affect the single colonies located in a given apiary and therefore invisibly bias colony infestation phenotypes. This bias may be lower in regions with lower colony density, providing suitable conditions to set up evaluation apiaries. To verify these hypotheses, we monitored mite infestation and reinvasion in experimental colonies, as well as infestation in neighboring colonies belonging to beekeepers in three areas with variable colony densities in the canton of Bern, Switzerland during three consecutive beekeeping seasons. Mite immigration fluctuated between apiaries and years and significantly contributed to colony infestation level. Depending on apiary and year, 17-48% of the mites present in the experimental colonies at the time of the summer oxalic acid final treatment potentially derived from mite immigration that had occurred since mid-spring. Mite immigration was not linked to local colony density or the infestation levels of beekeepers' colonies located within 2 km. Our results do not prove that apiaries for colony evaluation should necessarily be established in areas with low colony density. However, they highlight the high impact of beekeeping management practices on mite colony infestation levels.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional studies of McSTE24, McCYP305a1, and McJHEH, three essential genes act in cantharidin biosynthesis in the blister beetle (Coleoptera: Meloidae). 对水泡甲虫（鞘翅目： Meloidae）中参与胭脂虫素生物合成的三个重要基因 McSTE24、McCYP305a1 和 McJHEH 的功能研究。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae070

Yi Huang, Ling Shen, Fang Du, Zhongkang Wang, Youping Yin

{"title":"Functional studies of McSTE24, McCYP305a1, and McJHEH, three essential genes act in cantharidin biosynthesis in the blister beetle (Coleoptera: Meloidae).","authors":"Yi Huang, Ling Shen, Fang Du, Zhongkang Wang, Youping Yin","doi":"10.1093/jisesa/ieae070","DOIUrl":"10.1093/jisesa/ieae070","url":null,"abstract":"Cantharidin is a toxic defensive substance secreted by most blister beetles when attacked. It has been used to treat many complex diseases since ancient times and has recently regained popularity as an anticancer agent. However, the detailed mechanism of the cantharidin biosynthesis has not been completely addressed. In this study, we cloned McSTE24 (encoding STE24 endopeptidase) from terpenoid backbone pathway, McCYP305a1 (encoding cytochrome P450, family 305) and McJHEH [encoding subfamily A, polypeptide 1 and juvenile hormone (JH) epoxide hydrolase] associated to JH synthesis/degradation in the blister beetle Mylabris cichorii (Linnaeus, 1758, Coleoptera: Meloidae). Expression pattern analyses across developmental stages in adult males revealed that the expressions of 3 transcripts were closely linked to cantharidin titer exclusively during the peak period of cantharidin synthesis (20-25 days old). In contrast, at other stages, these genes may primarily regulate different biological processes. When RNA interference with double-stranded RNA suppressed the expressions of the 3 genes individually, significant reductions in cantharidin production were observed in males and also in females following McJHEH knockdown, indicating that these 3 genes might primarily contribute to cantharidin biosynthesis in males, but not in females, while females could self-synthesis a small amount of cantharidin. These findings support the previously hypothesized sexual dimorphism in cantharidin biosynthesis during the adult phase. McCYP305a1 collaborates with its upstream gene McSTE24 in cantharidin biosynthesis, while McJHEH independently regulates cantharidin biosynthesis in males.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11237990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141579983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomy of a pest control failure: introgression of cytochrome P450 337B3 alleles from invasive old-world bollworm into native corn earworm (Lepidoptera: Noctuidae). 害虫控制失败的剖析：细胞色素 P450 337B3 等位基因从入侵的旧世界棉铃虫引入本地玉米穗虫（鳞翅目：夜蛾科）。

IF 2.1 3区农林科学

Journal of Insect Science Pub Date : 2024-07-01 DOI: 10.1093/jisesa/ieae094

Marissa I Nufer, Brad S Coates, Craig A Abel, Patrick O'Neill, Morgan McCracken, Devendra Jain, Calvin A Pierce, James Glover, Tyler Towles, Gadi V P Reddy, Omaththage P Perera

{"title":"Anatomy of a pest control failure: introgression of cytochrome P450 337B3 alleles from invasive old-world bollworm into native corn earworm (Lepidoptera: Noctuidae).","authors":"Marissa I Nufer, Brad S Coates, Craig A Abel, Patrick O'Neill, Morgan McCracken, Devendra Jain, Calvin A Pierce, James Glover, Tyler Towles, Gadi V P Reddy, Omaththage P Perera","doi":"10.1093/jisesa/ieae094","DOIUrl":"10.1093/jisesa/ieae094","url":null,"abstract":"The establishment of invasive species populations can threaten the ecological balance in naïve habitats and impact agricultural production practices. Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) (old-world bollworm, OWBW) and Helicoverpa zea (corn earworm, CEW) were geographically separated prior to the 2013 report of OWBW invasion into South America. Introgression of OWBW-specific cytochrome P450 337B3 (CYP337B3) gene into CEW was repeatedly detected across South America and the Caribbean. Two hybrids were documented from Texas in 2019. In this study, screening insects collected in Olathe, CO, USA, where a failure of pyrethroids to control CEW damage to conventional sweetcorn in 2023 detected 28.6% of insects with the OWBW-specific CYP337B3 marker. Nucleotide sequencing of the CYP337B3 gene identified 73.1% and 26.9% of insects carried CYP337B3v2 and CYP337B3v6 alleles, respectively, and 0.15 overall frequency of CYP337B3 alleles. Based on prior data for distinct phylogeographic origins of CYP337B3v2 and v6 alleles, our results indicate Olathe samples were derived from 2 different introductions: An uncertain source of the v6 allele that was initially reported in West Africa and possibly South American or Caribbean origin of the globally distributed v2 allele. One of the 1618 individuals screened also carried a ribosomal RNA internal transcribed spacer 1 derived from OWBW. Local selection pressures at the Olathe location imposed by repeated pyrethroid exposures are likely attributed to the prevalence of CYP337B3, where control practices hasten the accumulation of phenotypic resistance by adaptive introgression. Pyrethroid and other resistance factors carried by invasive OWBW may continue to impact CEW management tactics across the Americas.","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":"24 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11441577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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