Moth caterpillar embryos and parasitoid egg infection as revealed in vivo and visualized by micro-CT scanning.

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY
Ryan J Smith, Liwen Han, Jacqueline C Bede, Pierre Dutilleul
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引用次数: 0

Abstract

The Lepidopteran pest Trichoplusia ni and the parasitoid wasp Trichogramma brassicae represent a fascinating biological system, important for sustainable agricultural practices but challenging to observe. We present a nondestructive method based on micro-CT scanning technology (CT: computed tomography) for visualizing the internal parts of caterpillar embryos and of emerging parasitoids from infected eggs. Traditional methods of microscopic observation of the opaque egg contents require staining or dissection. To explore the biological system nondestructively, we optimized the application of micro-CT scanning to construct 3-D images of insects in vivo.

显微ct扫描显示蛾毛虫胚胎和寄生蜂卵的体内感染情况。
鳞翅目害虫赤眼蜂(Trichoplusia ni)和寄生蜂(Trichogramma brassicae)代表了一个迷人的生物系统,对可持续农业实践很重要,但很难观察到。我们提出了一种基于微CT扫描技术(CT:计算机断层扫描)的无损方法,用于可视化毛毛虫胚胎的内部部分和从受感染的卵中出现的拟寄生虫。传统的显微镜观察不透明的鸡蛋内容物的方法需要染色或解剖。为了对生物系统进行无损探索,我们优化了微型ct扫描在昆虫体内三维成像的应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Insect Science
Journal of Insect Science 生物-昆虫学
CiteScore
3.70
自引率
0.00%
发文量
80
审稿时长
7.5 months
期刊介绍: The Journal of Insect Science was founded with support from the University of Arizona library in 2001 by Dr. Henry Hagedorn, who served as editor-in-chief until his death in January 2014. The Entomological Society of America was very pleased to add the Journal of Insect Science to its publishing portfolio in 2014. The fully open access journal publishes papers in all aspects of the biology of insects and other arthropods from the molecular to the ecological, and their agricultural and medical impact.
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