Journal of Infection and Chemotherapy最新文献

{"title":"Clinical and molecular characteristics of urinary catheter-associated Pseudomonas aeruginosa prostatic infection: A case series of four postoperative nosocomial infections","authors":"Shinnosuke Fukushima ,&nbsp;Takayuki Sano ,&nbsp;Takashige Kashimoto ,&nbsp;Hideharu Hagiya","doi":"10.1016/j.jiac.2025.102853","DOIUrl":"10.1016/j.jiac.2025.102853","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> is a causative pathogen of nosocomial catheter-associated urinary tract infections (CAUTI), but prostate involvement, including prostatitis and prostatic abscess, is rare. The clinical characteristics of <em>P. aeruginosa</em>-associated CAUTI with prostatic lesions, as well as the contribution of genetic backgrounds remain unclear. We describe four cases of urinary catheter-associated prostatic infection caused by <em>P. aeruginosa</em> following postoperative catheterization. All patients developed fever within 10 days after surgery, and three of the four patients developed bacteremia. Three patients were diagnosed with prostatic abscess by contrast-enhanced computed tomography or magnetic resonance imaging, while one case presented with prostatitis without abscess formation. Prostate-specific antigen levels were elevated over 20 ng/mL in all three measured cases. All patients were treated successfully with prolonged antibiotic therapy (28–39 days) without surgical drainage. Notably, all three abscess cases were successfully managed with fluoroquinolone-based combination therapy, highlighting its potential role in the management of prostatic abscesses. Three of four isolates were submitted for molecular investigations. All isolates harbored <em>exoT</em> and <em>exoY</em>, whereas <em>exoU</em> was absent. Biofilm-associated genes were detected in two cases, but not in the remaining case. Our findings suggested that <em>P. aeruginosa</em> strains carrying T3SS genes (<em>exoT</em> and <em>exoY</em>) potentially develop prostatic infections, independent of biofilm-associated genes. Host and iatrogenic factors, such as catheter manipulation, may play more critical roles in the development of prostatic pathology than strain-specific determinants. Assessment of prostate-specific antigen levels and early imaging may facilitate appropriate diagnosis and effective management when <em>P. aeruginosa</em> is detected as a cause of CAUTI.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102853"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145505065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The fourth nationwide surveillance of antimicrobial susceptibility against Neisseria gonorrhoeae from male urethritis in Japan, 2021","authors":"Mitsuru Yasuda ,&nbsp;Satoshi Takahashi ,&nbsp;Saori Oba ,&nbsp;Chikara Kumagai ,&nbsp;Masachika Saeki ,&nbsp;Mayumi Ono ,&nbsp;Yuuki Yakuwa ,&nbsp;Shinya Nirasawa ,&nbsp;Kanao Kobayashi ,&nbsp;Jun Miyazaki ,&nbsp;Koichiro Wada ,&nbsp;Masahiro Matsumoto ,&nbsp;Hiroshi Hayami ,&nbsp;Shingo Yamamoto ,&nbsp;Hiroshi Kiyota ,&nbsp;Junko Sato ,&nbsp;Naoki Hasegawa ,&nbsp;Tetsuya Matsumoto","doi":"10.1016/j.jiac.2025.102841","DOIUrl":"10.1016/j.jiac.2025.102841","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Neisseria gonorrhoeae</em>, an important pathogen of sexually transmitted infections, is resistant to all recommended antimicrobial agents, penicillins, tetracyclines, fluoroquinolones, oral third-generation cephalosporins and macrolides. Antimicrobial susceptibility surveillance of <em>N. gonorrhoeae</em> is needed to identify trends in its antimicrobial resistance. The fourth nationwide antimicrobial susceptibility surveillance of <em>N. gonorrhoeae</em> isolated from male urethritis was conducted by the Surveillance Committee of the Japanese Society of Infectious Diseases, the Japanese Society for Chemotherapy, and the Japanese Society of Clinical Microbiology.</div></div><div><h3>Methods</h3><div>Specimens of 1068 <em>N. gonorrhoeae</em> strains collected from male patients with or suspicious for urethritis at 50 facilities in 2021 were tested for antimicrobial susceptibility to 10 antimicrobial agents by CLSI methods.</div></div><div><h3>Results</h3><div>All strains were non-susceptible to penicillin G. The non-susceptible rate against cefixime was 7.4 %. At the Japanese dose, 39.6 % of strains were non-susceptible to cefixime. No strain was non-susceptible to ceftriaxone and spectinomycin. Susceptibility rates were ciprofloxacin, 24.7 %; tetracycline, 13.7 %; and azithromycin, 89.0 %. Only 0.47 % of isolated strains were resistant to all of cefixime, azithromycin, and ciprofloxacin. However, at the Japanese dose breakpoint, 31.8 % of strains were susceptible against azithromycin and 15.6 % of strains showed resistant to all of cefixime, azithromycin, and ciprofloxacin. Non-susceptibility rates against cefixime tended to be higher than the national average in Hokkaido, Kinki-Chugoku-Shikoku, and Tokai-Hokuriku regions, whereas those against ciprofloxacin tended to be higher in Kanto and Tokai-Hokuriku regions.</div></div><div><h3>Conclusion</h3><div>From these results, monotherapy with ceftriaxone or spectinomycin, which is currently recommended in Japanese guidelines for gonococcal infection, is considered appropriate. Continued gonococcal antimicrobial susceptibility surveillance is needed.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102841"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Campylobacter fetus meningitis and bacteremia: A case report in a post–liver transplant and post–splenectomy patient and a restructured literature review","authors":"Shiori Kitaya ,&nbsp;Hatsumi Otani ,&nbsp;Masahiro Yanagi ,&nbsp;Taketo Kubo ,&nbsp;Ryosuke Issiki ,&nbsp;Yoshinori Takahashi ,&nbsp;Megumi Oshima ,&nbsp;Hajime Kanamori","doi":"10.1016/j.jiac.2025.102862","DOIUrl":"10.1016/j.jiac.2025.102862","url":null,"abstract":"<div><h3>Background</h3><div><em>Campylobacter fetus</em> is a rare but serious pathogen, mainly affecting immunocompromised patients. Central nervous system infections, particularly meningitis, are uncommon; only one case has been reported after liver transplantation and none after splenectomy.</div></div><div><h3>Case presentation</h3><div>We report meningitis and bacteremia caused by <em>C. fetus</em> subsp. <em>fetus</em> (Cff) in a 70-year-old man with liver transplantation and splenectomy for hepatitis C virus-related cirrhosis, on long-term immunosuppression. He presented with intermittent fever; blood cultures grew <em>C. fetus</em>, and cerebrospinal fluid (CSF) cultures confirmed it. Therapy was changed from cefmetazole to meropenem and vancomycin, then de-escalated to high-dose ampicillin. After 23 days of antibiotics, he fully recovered without sequelae.</div></div><div><h3>Literature review</h3><div>The literature review included 36 adult cases identified through a PubMed search and our own case. Seventy-eight percent were male, with a median age of 49 years. Sixteen percent were immunocompromised, while 41 % were otherwise healthy. Exposure risks were reported in 43 %, though absent in 54 %. Of 38 isolates, 24 were subspeciated: Cff was most frequent (18/24, 75 %), followed by <em>C. fetus</em> subsp. <em>intestinalis</em> and <em>venerealis</em> (2 each), and single isolates of <em>jejuni</em> and <em>Spirillum serpens</em>. Relapse occurred in 22 %, and mortality was 5 %.</div></div><div><h3>Conclusion</h3><div><em>C. fetus</em> meningitis is rare and diagnostically challenging but should be considered in immunocompromised patients, including those with post-transplantation or post-splenectomy status. Infection may occur even without clear exposure. Both blood and CSF cultures are essential, with blood cultures often positive earlier. Prompt diagnosis and targeted therapy are crucial for favorable outcomes, and long-term follow-up is recommended due to relapse risk.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102862"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145530548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of asymptomatic paragonimiasis with mobile pulmonary lesions and concomitant liver fluke infection","authors":"Kenji Ohnishi ,&nbsp;Kana Sugimoto ,&nbsp;Hideki Wakabayashi ,&nbsp;Kohei Sasamoto ,&nbsp;Yuichiro Imai","doi":"10.1016/j.jiac.2025.102865","DOIUrl":"10.1016/j.jiac.2025.102865","url":null,"abstract":"<div><div>We report here an asymptomatic patient showing the location of lung lesions that differ between the first and subsequent chest CT scans. This raised suspicion of paragonimiasis, which ultimately led to its diagnosis. For physicians with limited or no experience with paragonimiasis, the observation that the location of lung lesions differs over time on imaging tests is an important clue in suspecting paragonimiasis. Moreover, this patient was co-infected with liver fluke. Even when one kind of parasitic infection is identified, it is important to be aware of other parasitic infections.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102865"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of hypoalbuminemia severity on total teicoplanin concentrations after 3 days loading dose: a retrospective observational study","authors":"Yuki Shimizu ,&nbsp;Kazuhiko Hanada ,&nbsp;Takeaki Watanabe ,&nbsp;Mari Araki ,&nbsp;Keisuke Aoyama ,&nbsp;Tomoya Sakai ,&nbsp;Keiko Kadota","doi":"10.1016/j.jiac.2025.102871","DOIUrl":"10.1016/j.jiac.2025.102871","url":null,"abstract":"<div><h3>Background</h3><div>Hypoalbuminemia is known to decrease total teicoplanin (TEIC) concentration; however, whether total TEIC concentration varies depending on hypoalbuminemia severity remains unclear. This study investigated the correlation between hypoalbuminemia severity and total TEIC concentrations after 3 days loading dose.</div></div><div><h3>Methods</h3><div>The study included patients (≥18 years old) administered TEIC between January 2017 and January 2025. Factors influencing total TEIC trough concentrations after 3 days loading dose (C_trough) were determined using multiple linear regression. Serum albumin (Alb) levels were stratified into three groups. The total and predicted free TEIC C_trough of patients administered standard and high loading dose regimens were compared among the groups.</div></div><div><h3>Results</h3><div>The total TEIC C_trough was negatively correlated with age (p = 0.04), and the estimated glomerular filtration rate (p &lt; 0.01) and positively correlated with body weight (p &lt; 0.01), serum Alb (p &lt; 0.01), and TEIC loading dose for within 3 days (p &lt; 0.01). The median total TEIC C_trough for the three groups differed significantly in both dosing regimens: standard, 10.5 [interquartile range, 9.2−13.0], 15.8 [12.1−18.6], and 17.1 [15.4−20.6] μg/mL; high, 12.5 [9.4−16.8], 17.2 [15.7−18.4], and 20.7 [17.4−22.8] μg/mL for Alb &lt;2.0 g/dL, 2.0 ≤ Alb &lt;3.0 g/dL, and Alb ≥3.0 g/dL, respectively. However, the predicted free TEIC C_trough was not significantly different among the Alb groups for either regimen.</div></div><div><h3>Conclusion</h3><div>The more severe the hypoalbuminemia, the lower the total TEIC C_trough; however, predicted free TEIC C_trough might not be affected. Overall, setting lower target total TEIC concentrations may be beneficial in case of hypoalbuminemia.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102871"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145564025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of two methods for detecting Streptococcus mitis/oralis-related species with meropenem-non-susceptibility","authors":"Kazuto Kuri ,&nbsp;Ryo Kinoshita-Daitoku ,&nbsp;Yoshichika Arakawa ,&nbsp;Keigo Shibayama ,&nbsp;Kouji Kimura","doi":"10.1016/j.jiac.2025.102846","DOIUrl":"10.1016/j.jiac.2025.102846","url":null,"abstract":"<div><div>Several studies have reported clinical isolates of <em>Streptococcus mitis, Streptococcus oralis,</em> and their related species (<em>S. mitis/oralis</em> group) with reduced carbapenem susceptibility. However, to date, no simple and accurate methods of detecting <em>S. mitis/oralis</em> group isolates with reduced carbapenem susceptibility have been reported. The meropenem ETEST misinterpreted 3 of 25 (12 %) meropenem-non-susceptible <em>S. mitis/oralis</em> group isolates as susceptible to meropenem. Therefore, we screened 38 disks containing 38 different β-lactams to detect <em>S. mitis/oralis</em> group strains with reduced carbapenem susceptibility using the disk-diffusion method. Additionally, using the agar dilution method to determine susceptibility, we identified the optimal antibiotic concentrations in agar medium to enable selective culture of meropenem-non-susceptible <em>S. mitis/oralis</em> group by culturing 25 meropenem-non-susceptible (meropenem minimum inhibitory concentration [MIC] &gt;0.5 μg/mL) and 24 meropenem-susceptible (meropenem MIC ≤0.5 μg/mL) <em>S. mitis/oralis</em> group isolates on agar containing different concentrations of six β-lactams. Meropenem-susceptible and non-susceptible <em>S. mitis/oralis</em> group isolates were successfully differentiated using oxacillin- and cefoxitin-containing disks. Moreover, cefdinir-, flomoxef-, meropenem-, and doripenem-containing disks showed clear differences in the growth inhibitory zone diameter between the meropenem-susceptible and meropenem-non-susceptible <em>S. mitis/oralis</em> group isolates. Media containing 2 μg/mL oxacillin, 4–16 μg/mL cefoxitin, 2 μg/mL cefdinir, 2–4 μg/mL flomoxef, 0.06–0.25 μg/mL meropenem, and 0.06–0.12 μg/mL doripenem allowed selective culture of meropenem-non-susceptible <em>S. mitis/oralis</em> group strains. The disk-diffusion method using six β-lactam-containing disks and selective culture agar medium are the first-reported simple and accurate methods of detecting meropenem-non-susceptible <em>S. mitis/oralis</em> group suitable for use in clinical microbiology laboratories.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102846"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key risk factors for syphilis among high-risk populations in urban areas of Japan","authors":"Yuka Yamagishi ,&nbsp;Marina Minami ,&nbsp;Natsuko Nakamura ,&nbsp;Yasuhiko Onoe ,&nbsp;Yoshitomo Kobori ,&nbsp;Ichiro Itoda ,&nbsp;Hiroshige Mikamo","doi":"10.1016/j.jiac.2025.102863","DOIUrl":"10.1016/j.jiac.2025.102863","url":null,"abstract":"<div><h3>Introduction</h3><div>We aimed to identify high-risk factors for syphilis infection in metropolitan areas, specifically Tokyo, to guide effective strategy development against syphilis epidemics.</div></div><div><h3>Methods</h3><div>From July 20, 2023, to January 26, 2024, a REDCap survey was administered to individuals tested for syphilis at three Tokyo clinics. Consent was obtained through an agreement option at initiation of the questionnaire permitting individuals to decline participation. By selecting the consent option and completing the survey, individuals agreed to participate in the study. This research was supported by a Ministry of Health, Labor and Welfare research grant (JPMH23HA2011) and approved by the Kochi University School of Medicine Ethics Committee (Approval No. 2023–44).</div></div><div><h3>Results</h3><div>A total of 215 participants were analyzed after excluding 13 from the initial cohort. Of these, 48.4 % tested positive for syphilis. Positive cases were more frequently individuals with a history of sexually transmitted infections (STIs) undergoing their first syphilis test. Symptoms strongly correlated with syphilis positivity (60.5 % among symptomatic participants). Multivariate analysis identified casual sexual relationships and a history of STIs as significant risk factors for syphilis positivity. The presence of symptoms was also significantly associated with syphilis positivity. Engagement in sex work and anal intercourse trended toward increased risk. Age, men having sex with men, and use of matchmaking apps exhibited no significant association.</div></div><div><h3>Conclusion</h3><div>This study identified risks related to sexual partners, sex industry involvement, and sexual activities. Promoting preventive measures among sex industry workers and patrons is crucial. Increased syphilis testing opportunities may aid in reducing disease prevalence.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102863"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cefazolin <3 g vs. 3 g treatment doses for cellulitis in patients who weigh ≥120 kg within a large, community health system via modified desirability of outcomes ranking (DOOR) methodology","authors":"Krista D. Gens ,&nbsp;Brandon R. Gagnon ,&nbsp;Kimberly C. Claeys ,&nbsp;Michael A. Wankum ,&nbsp;Elizabeth B. Hirsch","doi":"10.1016/j.jiac.2025.102833","DOIUrl":"10.1016/j.jiac.2025.102833","url":null,"abstract":"<div><h3>Background</h3><div>Cefazolin 3 g is recommended for patients ≥120 kg preoperatively; however, there is limited evidence to suggest 3 g for treatment dosing of cefazolin. This study aims to provide data on the efficacy and safety of high-dose cefazolin in obese patients with cellulitis using a modified Desirability of Outcomes Ranking (DOOR) methodology.</div></div><div><h3>Methods</h3><div>A multi-center, retrospective cohort study including adults weighing ≥120 kg who received ≥48 h of cefazolin for cellulitis in 2021 within a large, community health system was conducted. Patients were placed in a 3:1 ratio in &lt;3 g dosing (standard-dose; SD) and 3 g dosing (high-dose; HD) groups. Exclusion criteria included co-infections, bacteremia, diagnosis of bilateral lower extremity cellulitis, or receipt of &gt;48 h of concomitant antibiotics. The primary endpoint of efficacy and safety as determined by modified DOOR criteria.</div></div><div><h3>Results</h3><div>A total of 87 patients were included; 70 received SD and 17 received HD cefazolin. There were no differences in age, concomitant antibiotics, or severity of illness. Clinical success was higher in the HD vs SD group resulting in a 63.7% probability of a better outcome for a ≥120 kg patient receiving HD compared to SD (95% CI 50.4%, 75.2%, p = 0.0471).</div></div><div><h3>Conclusion</h3><div>Cefazolin 3 g was associated with a higher probability of a better outcome in patients ≥120 kg with cellulitis with no difference in adverse effects in this retrospective, cohort study utilizing modified DOOR criteria. Further study is necessary to confirm results.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102833"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145370257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing and in vitro characterization of a disseminated ST398 Staphylococcus aureus infection: A case report","authors":"Yosuke Sazumi ,&nbsp;Shinnosuke Fukushima ,&nbsp;Hideharu Hagiya ,&nbsp;Atsushi Kato ,&nbsp;Atsuhito Suyama ,&nbsp;Kohei Oguni ,&nbsp;Kazuyoshi Gotoh ,&nbsp;Shoko Kutsuno ,&nbsp;Junzo Hisatsune ,&nbsp;Motoyuki Sugai ,&nbsp;Shuma Tsuji ,&nbsp;Koji Iio ,&nbsp;Fumio Otsuka","doi":"10.1016/j.jiac.2025.102845","DOIUrl":"10.1016/j.jiac.2025.102845","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> potentially causes systemic infections such as disseminated abscesses and bloodstream infections, leading to high mortality rates. We herein describe a case of disseminated muscle abscesses caused by sequence type (ST) 398 methicillin-sensitive <em>S. aureus</em> (MSSA), along with <em>in vitro</em> investigation results for potential pathogenic factors. A 67-year-old healthy woman was admitted to our hospital with complaints of systemic body pain. Blood cultures identified MSSA and contrast-enhanced computed tomography revealed multiple muscle abscesses extending from her neck to her soles. She received antibiotic treatment with intravenous cephazolin and underwent repeated surgical drainage, and was finally discharged. Notably, the MSSA strain exclusively affected her muscle tissues, prompting us to perform genetic analysis to uncover the underlying reason. Short-read genome analysis revealed the isolate to be ST398, harboring <em>chp</em> and <em>scn</em> genes known for immune evasion from human immunity. However, no other known pathogenic factors were identified despite rigorous assays for biofilm formation, surface and cell wall proteins, protease production, and hyaluronidase activity. ST398 <em>S. aureus</em> is commonly isolated from livestock, and her prior experience of being flooded could be related to the disease onset. The present case underscores the possibility of severe ST398 MSSA infections in humans, even in the absence of direct animal exposure.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102845"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and detection of IgG antibodies against severe fever with thrombocytopenia syndrome virus among blood donors in southwestern part of Japan","authors":"Hitomi Nakamura ,&nbsp;Yasuko Sagara ,&nbsp;Midori Yamamoto ,&nbsp;Kayoko Fujimura ,&nbsp;Koji Matsuzaki","doi":"10.1016/j.jiac.2025.102844","DOIUrl":"10.1016/j.jiac.2025.102844","url":null,"abstract":"<div><h3>Introduction</h3><div>Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease caused by SFTS virus (SFTSV). No large-scale evaluation of the seroprevalence of SFTSV infection in the general population of Kyushu-Okinawa, where it is endemic, had ever been conducted. This study aimed to detect the antibody for SFTSV in blood donors and estimate the prevalence of anti-SFTSV IgG antibodies in Kyushu-Okinawa.</div></div><div><h3>Materials and methods</h3><div>Serum samples derived from 9987 blood donations were collected from December 2020 to January 2023. Anti-SFTSV antibodies was examined using the luciferase immunoprecipitation system (LIPS) with a recombinant nucleocapsid protein (rNP). LIPS-positive samples were further tested using indirect immunofluorescence assay (IFA) with rNP-expressed cells and enzyme-linked immunosorbent assay (ELISA) with SFTSV-infected cell lysate.</div></div><div><h3>Results</h3><div>Forty-three (0.43 %) cases showed positive results in LIPS, with relative luminescence units ratios of 1.02–1.82. Three of these cases were regarded IFA-positive, although two of above three showed weak fluorescence reactions with 1:40 serum dilution. One of the two samples with weak-positive IFA results were reactive to both of SFTSV infected and -uninfected cell lysate in ELISA. Therefore, 2 of 9987 samples were confirmed to be with anti-SFTSV IgG antibodies.</div></div><div><h3>Conclusions</h3><div>In our study, anti-SFTSV-IgG antibodies was detected in 0.02 % of Kyushu-Okinawa blood donors. The antibody assay using SFTSV rNP antigen can be adopted in general laboratories, thus facilitating expanded seroprevalence surveillance. Increasing awareness and popularizing testing would help correct diagnosis of SFTSV infection and provide precise epidemiological data.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 12","pages":"Article 102844"},"PeriodicalIF":1.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}