An evaluation of the modified carbapenem inactivation method (mCIM) in conjunction with EDTA or sodium mercaptoacetate, for detecting metallo β-lactamase production for Aeromonas species harboring blaCphA

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Infection and Chemotherapy Pub Date : 2025-03-11 DOI:10.1016/j.jiac.2025.102678

Kohei Uechi , Naoya Nishiyama , Wakako Arakaki , Ami Nakano , Ayumi Uechi , Tatsuya Nakamura , Kazuko Yamamoto , Shiro Maeda

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引用次数: 0

Abstract

Several Aeromonas species, including Aeromonas hydrophila, have been known to produce CphA, a chromosomal metallo β-lactamase (MBL). We aimed to evaluate the clinical usefulness of modified carbapenem inactivation method (mCIM) in conjunction with EDTA (eCIM) or sodium mercaptoacetate (SMA-mCIM) for MBL-producing Aeromonas species.

Fifty-six clinical isolates of Aeromonas species stored at the University of the Ryukyus Hospital were used in this study. The antimicrobial susceptibility testing was performed using a VITEK®2 AST-N404 card (bioMerieux, Marcy-l'Etoile, France) and the results were interpreted according to the CLSI guideline M45 3rd edition. The carbapenemase genes, namely bla_GES, bla_IMP, bla_KPC, bla_NDM, bla_OXA-48-like, bla_VIM, and bla_CphA, were detected by PCR. The production of carbapenemase was evaluated using the mCIM and the differentiation of MBL was eCIM and SMA-mCIM.

The resistance rates to imipenem and meropenem were 21.4 % (12/56) and 32.1 % (18/56), respectively. The mCIM showed a sensitivity of 100 % (33/33 bla_CphA positive isolates) and a specificity of 95.7 % (22/23 bla_CphA negative isolates) for the tested isolates. Among the 33 mCIM-positive isolates, 29 (87.9 %) for eCIM, and 6 (18.2 %) for SMA-mCIM were judged as MBL positive, respectively.

It is suggested that the mCIM in conjunction with EDTA is useful for detecting MBL production in Aeromonas species harboring bla_CphA.

查看原文本刊更多论文

改良碳青霉烯类失活方法（mCIM）与EDTA或巯基乙酸钠联合检测含黑氧单胞菌金属β-内酰胺酶产量的评价

一些气单胞菌，包括嗜水气单胞菌，已经知道产生CphA，一种染色体金属β-内酰胺酶（MBL）。我们的目的是评估改良碳青霉烯类灭活方法（mCIM）联合EDTA （eCIM）或巯基乙酸钠（SMA-mCIM）对产mbl气单胞菌的临床有效性。本研究使用了保存在琉球大学医院的56株气单胞菌临床分离株。使用VITEK®2 AST-N404卡（bioMerieux, Marcy-l'Etoile, France）进行抗菌药敏试验，并根据CLSI指南M45第3版对结果进行解释。PCR检测碳青霉烯酶基因blaGES、blaIMP、blaKPC、blaNDM、blaoxa -48 like、blaVIM、blaCphA。用mCIM评价碳青霉烯酶的产生，MBL分化为eCIM和SMA-mCIM。对亚胺培南和美罗培南的耐药率分别为21.4%（12/56）和32.1%（18/56）。mCIM检测blaCphA阳性菌株的敏感性为100%（33/33株），特异性为95.7%（22/23株）。33株mcim阳性分离株中，eCIM阳性29株（87.9%），SMA-mCIM阳性6株（18.2%）。这表明mCIM与EDTA结合可用于检测携带blaCphA的气单胞菌的MBL产量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Chemotherapy INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

4.10

自引率

4.50%

发文量

303

审稿时长

47 days

期刊介绍： The Journal of Infection and Chemotherapy (JIC) — official journal of the Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases — welcomes original papers, laboratory or clinical, as well as case reports, notes, committee reports, surveillance and guidelines from all parts of the world on all aspects of chemotherapy, covering the pathogenesis, diagnosis, treatment, and control of infection, including treatment with anticancer drugs. Experimental studies on animal models and pharmacokinetics, and reports on epidemiology and clinical trials are particularly welcome.