An evaluation of the modified carbapenem inactivation method (mCIM) in conjunction with EDTA or sodium mercaptoacetate, for detecting metallo β-lactamase production for Aeromonas species harboring blaCphA

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Infection and Chemotherapy Pub Date : 2025-03-11 DOI:10.1016/j.jiac.2025.102678

Kohei Uechi , Naoya Nishiyama , Wakako Arakaki , Ami Nakano , Ayumi Uechi , Tatsuya Nakamura , Kazuko Yamamoto , Shiro Maeda

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引用次数: 0

Abstract

Several Aeromonas species, including Aeromonas hydrophila, have been known to produce CphA, a chromosomal metallo β-lactamase (MBL). We aimed to evaluate the clinical usefulness of modified carbapenem inactivation method (mCIM) in conjunction with EDTA (eCIM) or sodium mercaptoacetate (SMA-mCIM) for MBL-producing Aeromonas species.

Fifty-six clinical isolates of Aeromonas species stored at the University of the Ryukyus Hospital were used in this study. The antimicrobial susceptibility testing was performed using a VITEK®2 AST-N404 card (bioMerieux, Marcy-l'Etoile, France) and the results were interpreted according to the CLSI guideline M45 3rd edition. The carbapenemase genes, namely bla_GES, bla_IMP, bla_KPC, bla_NDM, bla_OXA-48-like, bla_VIM, and bla_CphA, were detected by PCR. The production of carbapenemase was evaluated using the mCIM and the differentiation of MBL was eCIM and SMA-mCIM.

The resistance rates to imipenem and meropenem were 21.4 % (12/56) and 32.1 % (18/56), respectively. The mCIM showed a sensitivity of 100 % (33/33 bla_CphA positive isolates) and a specificity of 95.7 % (22/23 bla_CphA negative isolates) for the tested isolates. Among the 33 mCIM-positive isolates, 29 (87.9 %) for eCIM, and 6 (18.2 %) for SMA-mCIM were judged as MBL positive, respectively.

It is suggested that the mCIM in conjunction with EDTA is useful for detecting MBL production in Aeromonas species harboring bla_CphA.

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来源期刊

Journal of Infection and Chemotherapy INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

4.10

自引率

4.50%

发文量

303

审稿时长

47 days

期刊介绍： The Journal of Infection and Chemotherapy (JIC) — official journal of the Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases — welcomes original papers, laboratory or clinical, as well as case reports, notes, committee reports, surveillance and guidelines from all parts of the world on all aspects of chemotherapy, covering the pathogenesis, diagnosis, treatment, and control of infection, including treatment with anticancer drugs. Experimental studies on animal models and pharmacokinetics, and reports on epidemiology and clinical trials are particularly welcome.