Journal of Infection and Chemotherapy最新文献

{"title":"Association of Doses Based on Body Constitutional Parameters with the Efficacy of Micafungin in Candidemia.","authors":"Masaya Nagamizu, Yuji Hotta, Masato Noda, Daigaku Nakamura, Masayuki Hori, Yuto Otsuka, Ryuhei Takemoto, Yasuhiro Horita, Eri Wakita, Nobuyuki Morishita, Masahiro Kondo, Yoko Furukawa-Hibi, Kazunori Kimura","doi":"10.1016/j.jiac.2025.102654","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102654","url":null,"abstract":"<p><strong>Background: </strong>Invasive candidiasis is a life-threatening infection associated with high mortality, necessitating early and effective treatment. Micafungin, an echinocandin, is recommended as initial therapy for invasive candidiasis. However, the optimal micafungin dose relative to patients' body constitutional parameters (BCPs) remains unclear. This study aimed to evaluate the relationship between the dose of micafungin per BCPs (Dose/BCPs) and treatment outcomes.</p><p><strong>Methods: </strong>This two-center retrospective study included patients treated with micafungin who had confirmed positive blood cultures for Candida species between January 1, 2010, and December 31, 2020. We assessed the association between Dose/BCP and treatment success, as well as time to recovery following micafungin therapy.</p><p><strong>Results: </strong>Eighty-three patients were included in the analysis, with a median age of 78 years. The primary isolated Candida species were Candida albicans (n = 34), Candida parapsilosis (n = 19), and Candida glabrata (n = 16). The treatment success rate was 44.6% and was significantly associated with age ≥ 75 years. Although no significant differences in Dose/BCP were observed between the success and failure groups, patients with a Dose/BSA ≥ 100 mg/m² experienced a significantly shorter time to recovery with micafungin therapy.</p><p><strong>Conclusion: </strong>Our study identified an association between Dose/BSA and the time to recovery with micafungin therapy. While some missing data, including APACHE-II scores, limit the robustness of the findings because of the retrospective design, dose adjustment to achieve Dose/BSA ≥ 100 mg/m² may be beneficial in antifungal stewardship. This adjustment could reduce treatment duration with this broad-spectrum antifungal agent.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102654"},"PeriodicalIF":1.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infective Native Aortic and Iliac Artery Aneurysms: Clinical Profiles and Short-Term Outcomes from a Single-Center Cohort.","authors":"Natsuki Shinya, Masafumi Seki, Haruka Karaushi, Toshihisa Asakura, Akihiro Yoshitake, Kotaro Mitsutake","doi":"10.1016/j.jiac.2025.102644","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102644","url":null,"abstract":"<p><strong>Introduction: </strong>Infective native aortic and iliac artery aneurysms, although rare, have high mortality rates of 21%-44%. Diagnosis is often delayed owing to nonspecific symptoms. Treatment includes surgical intervention and antimicrobial therapy. This study aimed to describe the clinical characteristics and short-term outcomes of 32 patients with infective aneurysms at a single center.</p><p><strong>Methods: </strong>This retrospective study, conducted at the Saitama International Medical Center from January 2011 to December 2020, included patients diagnosed with infective native aortic and iliac artery aneurysms. The patients' clinical data, microbiological and radiological findings, treatment methods, and outcomes were collected and analyzed.</p><p><strong>Results: </strong>Of the 32 patients, 56.3% presented with fever (≥37.5 °C) and 87.5% exhibited nonspecific symptoms, such as pain and loss of appetite. Blood cultures were positive in 18 patients (58.1%), with Staphylococcus aureus being the predominant pathogen, isolated in 11 patients. Pathogens in 5 of the 11 cases (45%) were resistant to methicillin. Surgery was performed in 75% of patients, with 20 undergoing open surgical repair (OSR) and 4 undergoing endovascular treatment (EVT). The 1-year mortality rates of the patients who underwent OSR and EVT were 23.5% (4/17) and 0% (0/4), respectively. Postoperative infection-related complications occurred in 25% of patients who underwent OSR. For nonsurgical patients, the 30-day and 1-year mortality rates were 25% (2/8) and 85.7% (6/7), respectively.</p><p><strong>Conclusion: </strong>The findings of this study highlight the high mortality rates associated with infective aneurysms. S. aureus was the predominant pathogen, differing from trends observed in other Asian regions.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102644"},"PeriodicalIF":1.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatal pneumonia with repeated detection of Talaromyces columbinus two years after haploidentical transplantation.","authors":"Yuya Nakata, Nozomu Yoshino, Machiko Kusuda, Shun-Ichi Kimura, Akari Matsuoka, Tomohiro Meno, Takuto Ishikawa, Yuhei Nakamura, Masakatsu Kawamura, Junko Takeshita, Shunto Kawamura, Yukiko Misaki, Kazuki Yoshimura, Ayumi Gomyo, Masaharu Tamaki, Yasutaka Hoshino, Takayuki Shinohara, Yoshitsugu Miyazaki, Hideki Nakasone, Shin-Ichi Kako, Yoshinobu Kanda","doi":"10.1016/j.jiac.2025.102649","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102649","url":null,"abstract":"<p><p>Talaromyces columbinus was previously reported in two patients with lung infections under the name Penicillium piceum and one case of dual infection with Aspergillus calidoustus was recently reported.; currently, no treatment has been established. We identified a 61-year-old woman with fatal pneumonia with repeated detection of T. columbinus that developed two years after haploidentical transplantation using alemtuzumab for chronic myeloid leukemia in the blast phase. Seven months after transplantation, her minimal residual disease (MRD) turned positive. Thus, ponatinib was restarted, which resulted in MRD becoming negative again. Nine months after transplantation, she developed autoimmune hemolytic anemia (AIHA); treatment with prednisone (PSL) 35 mg was started. PSL was discontinued one year ten months after transplantation, but was resumed at 5 mg after relapse one year eleven months after transplantation. Two years after transplantation, she developed cough, and a CT scan showed bilateral pulmonary infiltrates. Initiation of antibiotics, voriconazole (VRCZ), posaconazole (PSCZ) and liposomal amphotericin B (L-AMB) did not improve her condition. Sputum culture detected Penicillium species, which was identified as T. columbinus by polymerase chain reaction (PCR). Since the minimal inhibitory concentration (MIC)/ minimal effective concentration (MEC) ratio was lower for echinocandins, micafungin (MCFG) was added to L-AMB. However, the patient died of respiratory failure on day 38 of admission. This is the first reported case of T. columbinus infection in Japan. Managing this infection is challenging due to the lack of established diagnostic methods and treatments. Proactive diagnostic testing and case accumulation are needed.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102649"},"PeriodicalIF":1.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive pneumococcal disease caused by CO₂-dependent Streptococcus pneumoniae serotype 24F sequence type 162: a case report.","authors":"Takahiko Niwa, Takehisa Matsumoto, Misako Ohkusu, Naruhiko Ishiwada, Kazunori Fukushima, Suguru Hiramoto, Azusa Uchida, Katsuhiko Tsunekawa, Kiyohiro Oshima, Takao Kimura","doi":"10.1016/j.jiac.2025.102653","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102653","url":null,"abstract":"<p><p>We report the case of a man in his 70s who developed invasive pneumococcal disease (IPD) caused by CO₂-dependent Streptococcus pneumoniae. He was admitted with suspected pneumonia based on chest X-ray and computed tomography imaging findings. Ceftriaxone treatment was initiated following the collection of sputum samples and two sets of blood cultures. The antimicrobial treatment was then changed to sulbactam/ampicillin (SBT/ABPC) because urine pneumococcal antigen testing performed on admission was positive. The following day, CO₂-dependent S. pneumoniae was isolated from the blood cultures and sputum sample, leading to a diagnosis of IPD. The patient continued SBT/ABPC treatment (6 g/day) but remained febrile and died 4 days after admission. Serotyping and whole-genome sequence analysis of the isolate revealed that it was serotype 24F and sequence type (ST) 162. A c.536C>T mutation was identified in murF of the isolate compared with S. pneumoniae Spain9V-3. The isolation of CO₂-dependent S. pneumoniae serotype 24F ST162 from pediatric patients in Japan was recently reported. However, to the best of our knowledge, this is the first reported IPD case in an elderly patient caused by CO₂-dependent S. pneumoniae serotype 24F ST162. Thus, even in adults with IPD, caution should be exercised going forward.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102653"},"PeriodicalIF":1.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isepamicin alone as antimicrobial prophylaxis for transrectal prostate needle biopsy: \" Do we still need levofloxacin? \".","authors":"Hiroki Yamada, Kojiro Tashiro, Yusuke Takahashi, Mariko Honda, Hajime Ohnuma, Keiji Yasue, Mahito Atsuta, Kei-Ichiro Miyajima, Mimu Ishikawa, Yuki Takiguchi, Hiroshi Kiyota, Takahiro Kimura","doi":"10.1016/j.jiac.2025.102645","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102645","url":null,"abstract":"<p><p>The purpose of this study was to investigate whether conventional levofloxacin (LVFX) administration is unnecessary for transrectal ultrasound-guided prostate needle biopsy (TRP-Bx) in view of the increase in LVFX-resistant Escherichia coli and appropriate use of antibiotics. The study included 636 cases undergoing TRP-Bx, divided into two groups based on the prophylactic antibiotic regimen. Group 1 (n = 308) received both oral levofloxacin (LVFX) 500 mg and intravenous isepamicin (ISP) 400 mg. Group 2 (n = 328) received only intravenous ISP 400 mg. Biopsies involved sampling 12 cores using an 18G needle. A high-risk subgroup included patients with a large prostate (> 75 ml), severe dysuria, diabetes mellitus, or steroid use. Significantly more high-risk cases were in Group 2 than in Group 1 (35.7% vs. 24.4%, p = 0.003). Febrile genitourinary tract infections (fGUTIs) occurred in three patients (0.5%), with no significant difference between the groups (0.3% in Group 1 vs. 0.6% in Group 2). No fGUTI complications were noted among high-risk cases in either group. Of the fGUTI cases, one involved LVFX-resistant E. coli; another involved E. coli susceptible to both LVFX and amikacin, isolated from blood. The single- and short-duration intravenous dose of ISP 400 mg would appear to be one of possible options in preventing TRP-Bx-related fGUTIs in both low-risk and high-risk patients.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102645"},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Days of antibiotic spectrum coverage (DASC) as predictors of recurrent Clostridioides difficile infection: A retrospective cohort study.","authors":"Nobuaki Mori, Yuichi Shibata, Jun Hirai, Nobuhiro Asai, Hiroshige Mikamo","doi":"10.1016/j.jiac.2025.102650","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102650","url":null,"abstract":"<p><strong>Background: </strong>Broad-spectrum antibiotic use increases the risk of Clostridioides difficile infection (CDI), with recurrence rates varying by antibiotic type, spectrum, and treatment duration. We assessed CDI recurrence risk using the days of antibiotic spectrum coverage (DASC) score, considering antibiotic spectrum and use duration.</p><p><strong>Methods: </strong>We retrospectively analyzed patients with hospital-acquired CDI. A logistic regression analysis was used to evaluate CDI recurrence, incorporating three variables: DASC score prior to CDI diagnosis, type of anti-CDI drugs, or DASC score after CDI diagnosis.</p><p><strong>Results: </strong>Overall, 246 patients were included, with 31 (12.6%) cases of recurrence. Median DASC scores within 30 days prior to CDI diagnosis were higher in the recurrent group than in the non-recurrent group (128 [interquartile range: 106-217] vs. 80 [interquartile range: 39-142], p<0.01). Using the lowest quartile of DASC scores as the reference, the analysis indicated higher relative risks of CDI recurrence in the upper quartiles. However, daily DASC scores post-CDI diagnosis did not correlate with recurrence. Compared to metronidazole, fidaxomicin lowered the risk of CDI recurrence (relative risk 0.2, 95% confidence interval: 0.1-0.8, p=0.03).</p><p><strong>Conclusions: </strong>The DASC score within 30 days before CDI diagnosis appears to be a predictive risk factor for CDI recurrence.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102650"},"PeriodicalIF":1.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First reported case of disseminated Cunninghamella phaeospora infection with multidrug resistance in acute myeloid leukemia","authors":"Tomohito Shimada ,&nbsp;Akira Watanabe ,&nbsp;Kaori Akita ,&nbsp;Kana Bando ,&nbsp;Atsushi Takahata ,&nbsp;Kazuhiro Ishikawa ,&nbsp;Shigeo Toyota","doi":"10.1016/j.jiac.2025.102646","DOIUrl":"10.1016/j.jiac.2025.102646","url":null,"abstract":"<div><div>Mucormycosis is a severe mold infection primarily affecting immunocompromised patients. Neutropenia, steroid use, hyperglycemia, and diabetes are recognized as significant risk factors. <em>Cunninghamella</em> species are rare pathogenic fungi associated with high mortality rates and multidrug resistance. However, there have been no reports of <em>C. phaeospora</em> being identified as the causative agent of clinical infection. We report a case of a 71-year-old man who developed right middle lobe pneumonia during salvage induction therapy for relapsed acute myeloid leukemia. Based on the clinical course, mucormycosis was suspected, and antifungal therapy was initiated with isavuconazole (200 mg every 8 hours for six doses, followed by 200 mg daily) and later switched to liposomal amphotericin B (5 mg/kg/day). Despite these interventions, the patient's respiratory failure progressed, culminating in a fatal hemorrhagic infarction of the right lung. An autopsy revealed invasive fungal involvement in multiple organs, including the lungs and liver. Genetic identification of the isolated fungi demonstrated <em>C. phaeospora</em>, confirming disseminated <em>C. phaeospora</em> infection. Susceptibility testing showed high Minimum Inhibition Concentrations/Minimum Effective Concentrations to all tested antifungal agents. This is the first reported case of disseminated infection caused by <em>C. phaeospora</em> with multidrug resistance. This case highlights the diagnostic and therapeutic challenges associated with rare pathogenic fungi. It underscores the importance of early identification of Mucorales, including susceptibility testing, to optimize antifungal therapy and consider appropriate surgical interventions. Further research is required to elucidate the mechanisms of antifungal resistance and clinical characteristics of <em>C. phaeospora</em>.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 4","pages":"Article 102646"},"PeriodicalIF":1.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of transurethral enucleation with bipolar for benign prostatic hyperplasia patients with chronic prostatitis/chronic pelvic pain syndrome – Single center retrospective study","authors":"Yoshikazu Togo,&nbsp;Shimpei Yoshioka,&nbsp;Yohei Kaizuka,&nbsp;Seiji Nagasawa","doi":"10.1016/j.jiac.2025.102647","DOIUrl":"10.1016/j.jiac.2025.102647","url":null,"abstract":"<div><h3>Introduction</h3><div>Transurethral prostate surgery is the standard procedure for patients with benign prostatic hyperplasia (BPH), while the efficacy of surgical treatment for patients with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) remains unclear. This study was conducted to evaluate the usefulness of transurethral enucleation with bipolar (TUEB) of the prostate for BPH patients with CP/CPPS.</div></div><div><h3>Methods</h3><div>Between February 2018 and May 2024, 53 BPH patients with CP/CPPS underwent TUEB of the prostate at our institution and were followed for more than three months. Changes in the National Institutes of Health chronic prostatitis symptom index (NIH-CPSI) score before and after surgery were retrospectively examined. Patients with bladder stones or indwelling urethral catheters before surgery were excluded.</div></div><div><h3>Results</h3><div>Mean values for NIH-CPSI total, pain domain, urinary symptoms domain, and quality of life impact domain scores at the baseline were 21.7, 7.0, 6.4, and 8.3, respectively. Follow-up examinations showed scores of 11.1, 2.5, 3.3, and 5.3, respectively, after one month, 7.9, 1.3, 2.9, and 3.6, respectively, after three months, 4.8, 0.5, 1.7, and 2.5, respectively, after six months, and 4.5, 0.6, 1.7, and 2.3, respectively, after 12 months, with all domain scores significantly reduced as compared with the baseline (P &lt; 0.001).</div></div><div><h3>Conclusions</h3><div>The present results suggest that TUEB of the prostate is effective for alleviating not only urinary symptoms but also pain in BPH patients with CP/CPPS.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 4","pages":"Article 102647"},"PeriodicalIF":1.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of fosfomycin susceptibility testing methods: A focus on multidrug-resistant Klebsiella pneumoniae using ECOFF values","authors":"Zeycan Semerci ,&nbsp;Fatih Mehmet Akıllı ,&nbsp;Arzu İlki","doi":"10.1016/j.jiac.2025.102643","DOIUrl":"10.1016/j.jiac.2025.102643","url":null,"abstract":"<div><h3>Introduction</h3><div>We aimed to evaluate the performance of two methods; disc diffusion and gradient test with the gold standard agar dilution method in determining the susceptibility of fosfomycin, in multidrug-resistant (MDR) <em>K. pneumoniae</em> isolates causing urinary tract infections.</div></div><div><h3>Methods</h3><div><em>K. pneumoniae</em> producing carbapenemase and extended-spectrum beta-lactamase (ESBL) isolated from urine samples submitted to the clinical microbiology laboratory were included in the study. The isolates were tested using gradient test (MTS, Liofilchem, Italy) and disc diffusion (Oxoid, UK). Agar dilution was employed as the reference method. Since there is no MIC value for <em>K. pneumoniae</em> in EUCAST, epidemiological cut-off values (ECOFFs) were determined and susceptibility and error rates were calculated.</div></div><div><h3>Results</h3><div>In this study, among the 251 ESBL-positive <em>K. pneumoniae</em> isolates, 20(8 %) were also positive for carbapenemase. The ECOFF was determined as 128 mg/L for <em>K. pneumoniae</em>. When all study isolates (n:251) were considered, 87.6%(220/251) were wild-type (WT) for fosfomycin (MIC≤128 mg/L). Among ESBL-positive but carbapenemase-negative isolates (n:231), 87.8%(203/231) were WT for fosfomycin, and among ESBL and carbapenemase-positive isolates (n:20), 85.0%(17/20) were WT. The MIC_50/90 values were determined to be 8/256 mg/L. When compared to agar dilution, the categorical agreement was 96.8% for the gradient test and 94.8 % for disc diffusion. While the gradient test showed a 16.1% very major error (VME) rate with no major errors (ME), disk diffusion revealed 35.4 % VME rate and 5.8 % ME rate.</div></div><div><h3>Conclusion</h3><div>A significant proportion of ESBL-positive <em>K. pneumoniae</em> isolates were WT for fosfomycin. The gradient test with 96.8% categorical agreement appears to be a good alternative, but agar dilution remains the gold Standard for reference laboratories.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 4","pages":"Article 102643"},"PeriodicalIF":1.9,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fully automated identification of Neisseria meningitidis using the BD MAX system in a clinical laboratory setting: A preliminary study","authors":"Rika Inose ,&nbsp;Yoshifumi Uwamino ,&nbsp;Wataru Aoki ,&nbsp;Yuka Kamoshita ,&nbsp;Mika Nagata ,&nbsp;Osamu Ishihara ,&nbsp;Hiromitsu Yokota ,&nbsp;Hiromichi Matsushita","doi":"10.1016/j.jiac.2025.102648","DOIUrl":"10.1016/j.jiac.2025.102648","url":null,"abstract":"<div><div>The rapid and accurate identification of <em>Neisseria meningitidis</em>, a pathogen that causes invasive infections and meningitis, is crucial for its effective clinical management and infection control. However, identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry may misidentify other <em>Neisseria</em> species as <em>N. meningitidis</em>, thus necessitating confirmatory tests based on biochemical properties. These tests require high bacterial concentrations that are achieved through subculturing, which can increase biosafety risks in laboratories.</div><div>In this study, we developed a real-time polymerase chain reaction detection system for <em>N. meningitidis</em> using the BD MAX automated genetic testing platform. We then evaluated its accuracy using 25 strains of clinically isolated <em>Neisseria</em> species, including <em>N. meningitidis</em>. Our detection results were in full agreement with those of sequencing-based identification, with a minimum detection sensitivity of 10 CFU/mL. The BD MAX system completes all measurements in a closed system, allowing for the rapid and precise identification of <em>N. meningitidis</em> while reducing laboratory biosafety risks.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 4","pages":"Article 102648"},"PeriodicalIF":1.9,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}