Journal of Infection and Chemotherapy最新文献

{"title":"A nationwide epidemiological survey of coronavirus disease 2019-associated pulmonary aspergillosis in Japan","authors":"Yuya Ito ,&nbsp;Takahiro Takazono ,&nbsp;Hotaka Namie ,&nbsp;Masato Tashiro ,&nbsp;Hiroshi Kakeya ,&nbsp;Yoshitsugu Miyazaki ,&nbsp;Hiroshi Mukae ,&nbsp;Hiroshige Mikamo ,&nbsp;Tomoo Fukuda ,&nbsp;Kazutoshi Shibuya ,&nbsp;Koichi Izumikawa","doi":"10.1016/j.jiac.2025.102739","DOIUrl":"10.1016/j.jiac.2025.102739","url":null,"abstract":"<div><h3>Background</h3><div>Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) is a severe complication of COVID-19 with a poor prognosis. In this study, we aimed to analyze the nationwide epidemiology of CAPA in Japan.</div></div><div><h3>Methods</h3><div>This nationwide retrospective study involved data of 98 patients with CAPA reported in 221 hospitals in Japan between January 2020 and August 2023, using both online and paper-based questionnaires. We investigated the clinical characteristics of CAPA, assessed outcomes using Kaplan–Meier curves, and identified independent predictors of 90-day mortality using Cox proportional hazards analysis.</div></div><div><h3>Results</h3><div>The median age of the patients with CAPA was 71 years, and 71.4 % were men. The positivity rates for β-D-glucan and serum galactomannan antigen were 59.1 % and 68.4 %, respectively, while chest computed tomography revealed cavities in 25.5 % of patients. The 30-day and 90-day mortality rates were 50.0 % and 55.1 %, respectively. Kaplan–Meier analysis showed that the mortality rate of patients treated with echinocandin monotherapy was significantly higher than that of patients treated with other antifungals (log-rank test, <em>p</em> = 0.04). Multivariate analysis identified a neutrophil count of ≥10,000/μL (hazard ratio [HR]: 2.67, 95 % confidence interval [CI]: 1.49–4.89) and echinocandin monotherapy (HR: 2.06, 95 % CI: 1.01–3.93) as independent predictors of 90-day mortality. Patients treated with echinocandin monotherapy were older, and <em>Aspergillus</em> spp. was not isolated in half of these patients.</div></div><div><h3>Conclusion</h3><div>This study provides a comprehensive overview of CAPA in Japan. Appropriate antifungal therapy is essential to improve the prognosis of patients with CAPA.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102739"},"PeriodicalIF":1.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics of Pediatric Parapertussis: A Single-Center Case-Control Study.","authors":"Ken Okamoto, Takanori Funaki, Satsuki Kidokoro, Akira Ishiguro, Chikara Ogimi","doi":"10.1016/j.jiac.2025.102740","DOIUrl":"https://doi.org/10.1016/j.jiac.2025.102740","url":null,"abstract":"<p><p>Parapertussis in children is often regarded as a disease similar to pertussis but remains underrecognized in Japan, where it is not a notifiable disease. This study aimed to elucidate its clinical and epidemiological features. A retrospective study was conducted at a pediatric center in Tokyo from October 2020 to November 2023. Patients under 18 years of age who tested positive for Bordetella parapertussis using the FilmArray® Respiratory Panel 2.1 were included. Data on demographics, clinical features, and laboratory results were collected from electronic records. Patients were classified into mono-infection (B. parapertussis only) or co-infection (with other pathogens). Fisher's exact and Mann-Whitney U tests were used for group comparisons, as appropriate. Among 11,384 tests, 45 were positive for B. parapertussis, first detected in August 2021. By 2023, the detection rate per test was 1.0% (40/3,941). The median age was 3.7 years, with 51.1% male. Of the cases, 82.2% had co-infections, most commonly with human rhinovirus/enterovirus (59.5%). Symptoms were mild, with cough in 71.1% and fever ≥38°C in 46.6%, and no patients required mechanical ventilation or intensive care. Azithromycin was administered to 64.4% of patients. Lymphocyte counts were significantly lower in mono-infected patients than in co-infected patients (median [interquartile range]: 1,065/μL [626-1,611] vs. 5,289/μL [3,637-9,655], respectively, P= 0.02). This study shows that parapertussis generally follows a mild clinical course but is often found with other pathogens. Given the lack of nationwide surveillance for parapertussis in Japan, more data are needed to assess the disease burden and develop management strategies.</p>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":" ","pages":"102740"},"PeriodicalIF":1.9,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successive SARS-CoV-2 mutations in a highly susceptible host: A case report of persistent viremia and fatality","authors":"Yuko Danzuka ,&nbsp;Keiji Nakamura ,&nbsp;Shinya Matsumoto ,&nbsp;Yoshitaka Etoh ,&nbsp;Sho Yamasaki ,&nbsp;Koji Takayama ,&nbsp;Masayuki Murata ,&nbsp;Nobuyuki Shimono","doi":"10.1016/j.jiac.2025.102737","DOIUrl":"10.1016/j.jiac.2025.102737","url":null,"abstract":"<div><div>A 71-year-old female with post-treatment follicular lymphoma developed COVID-19 with persistent viremia. Despite the initiation of remdesivir on day 23, she remained SARS-CoV-2 PCR-positive and ultimately succumbed to respiratory failure on day 79. Whole-genome analysis of RNA extracted from a post-mortem nasopharyngeal swab identified multiple mutations, including P323L/G671S, which enhances viral replication, and V792I, associated with remdesivir resistance. This case highlights the challenges of managing persistent SARS-CoV-2 infection in immunocompromised patients and underscores the impact of delayed treatment and antiviral resistance.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102737"},"PeriodicalIF":1.9,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolonged Bacillus cereus bacteremia: case report and literature review","authors":"Yuki Hirao ,&nbsp;Hiroshi Morioka ,&nbsp;Tomomasa Agata ,&nbsp;Masaki Iimura ,&nbsp;Shunichi Taki ,&nbsp;Tetsuya Yagi","doi":"10.1016/j.jiac.2025.102736","DOIUrl":"10.1016/j.jiac.2025.102736","url":null,"abstract":"<div><div>A 77-year-old man, initially diagnosed with pneumonia presented with fever and was administered continuous amino acid preparations and meropenem. <em>Bacillus cereus</em> was isolated from two separate sets of blood cultures, leading to a diagnosis of catheter-related bloodstream infection. Despite treatment with teicoplanin (TEIC) and additional antibiotics, <em>B. cereus</em> was continuously yielded from blood cultures for approximately four weeks. Ultimately, the patient was successfully treated with a combination of TEIC and clindamycin for six weeks, along with gentamicin for two weeks. This case report further reviews 17 cases of persistent <em>B. cereus</em> bacteremia. These cases provide valuable insights into the management of challenging cases of <em>B</em>. <em>cereus</em> bacteremia.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102736"},"PeriodicalIF":1.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144139308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epstein–Barr virus-associated infectious mononucleosis exhibits substantially higher non-treponemal test titers in biological false-positive reactions","authors":"Naoki Matsuura ,&nbsp;Takashi Matono ,&nbsp;Miho Kuroki ,&nbsp;Kenta Saitou","doi":"10.1016/j.jiac.2025.102738","DOIUrl":"10.1016/j.jiac.2025.102738","url":null,"abstract":"<div><h3>Objectives</h3><div>Biological false-positive (BFP) reactions in rapid plasma reagin (RPR) tests can complicate syphilis screening interpretation. Although Epstein–Barr virus-associated infectious mononucleosis (EBV-IM) is a known cause of BFP reactions, the characteristics of RPR titers in such cases remain unclear. This study aimed to compare the RPR titers in BFP reactions between patients with EBV-IM and those with other underlying conditions.</div></div><div><h3>Methods</h3><div>We retrospectively analyzed the RPR and <em>Treponema pallidum</em> hemagglutination assay (TPLA) results of all tests performed at a community hospital in Japan between April 2016 and March 2024. BFP reactions were defined as positive RPR and negative TPLA results. Clinical characteristics and RPR titers were compared between EBV-IM and other causes of BFP reactions.</div></div><div><h3>Results</h3><div>Among 154 confirmed BFP cases, EBV-IM cases (n = 7) showed significantly higher RPR titers than other underlying conditions (n = 147) (median 28.5 relative units [RU], interquartile range [IQR]: 11.2–31.2 vs. 1.9 RU, IQR: 1.2–4.3, <em>p</em> &lt; 0.001), despite a significantly younger age (median age 18 years, IQR: 17.4–25 vs. 72 years, IQR, 44.5–82, <em>p</em> &lt; 0.001). Among all BFP cases, neoplasms were the most common underlying condition (22.1 %), followed by digestive diseases (8.4 %) and infectious diseases (7.8 %).</div></div><div><h3>Conclusions</h3><div>EBV-IM is associated with distinctively high RPR titers in BFP reactions, in contrast to the typically low titers observed under other conditions. Clinicians should be aware that high RPR titers can occur during BFP reactions, particularly in young patients with suspected EBV infection.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102738"},"PeriodicalIF":1.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of antibiotic adverse events","authors":"Toshiharu Urakami,&nbsp;Takashi Matono","doi":"10.1016/j.jiac.2025.102735","DOIUrl":"10.1016/j.jiac.2025.102735","url":null,"abstract":"<div><h3>Purpose</h3><div>Antibiotic-associated adverse drug events (ADEs) are common and diverse in clinical practice, yet healthcare professionals often lack sufficient understanding of them. An ill-considered change to an alternative agent whent symptoms of suspected ADEs appear can lead to excessive overuse and misuse of antibiotics. Proper management of ADEs is part of antimicrobial stewardship.</div></div><div><h3>Areas covered</h3><div>This review describes clinical characteristics, diagnosis and management of ADEs, featuring drug fever, rash, nephrotoxicity, liver injury, interstitial pneumonitis, rhabdomyolysis, encephalopathy, QT prolongation, thrombocytopenia and neutropenia.</div></div><div><h3>Summary</h3><div>Healthcare professionals should be familiar with the typical symptoms, time course and likely causative agent for each adverse reaction. Early detection and proper judgement are important. In sever case, early discontinuation of the causative agent and proper treatment are required. If it is not fatal, continuing the suspected agent and careful monitoring may help identify the causative agent. Especially in penicillin allergies, it is important to remember that most patients labeled as allergic are, in fact, misclassified.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102735"},"PeriodicalIF":1.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of switching to long-acting cabotegravir + rilpivirine versus continuing bictegravir/emtricitabine/tenofovir alafenamide in Japanese participants: 12-month results from the phase 3b randomized SOLAR trial","authors":"Eisuke Adachi ,&nbsp;Yoshiyuki Yokomaku ,&nbsp;Dai Watanabe ,&nbsp;Hiroyuki Gatanaga ,&nbsp;Shinichi Oka ,&nbsp;Takuma Shirasaka ,&nbsp;Rumi Wakatabe ,&nbsp;Nadine Chamay ,&nbsp;Kenneth Sutton ,&nbsp;Denise Sutherland-Phillips ,&nbsp;Rimgaile Urbaityte ,&nbsp;Ronald D'Amico ,&nbsp;Jean van Wyk","doi":"10.1016/j.jiac.2025.102734","DOIUrl":"10.1016/j.jiac.2025.102734","url":null,"abstract":"<div><div>In the phase 3b SOLAR study, switching to long-acting cabotegravir + rilpivirine (CAB+RPV LA) administered every 2 months (Q2M) was non-inferior to continuing daily oral bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF). We present a post hoc analysis of Japanese participants. SOLAR is a randomized (2:1), open-label, multicenter, non-inferiority study of virologically suppressed participants switching to CAB+RPV LA Q2M (with or without oral lead-in) versus continuing BIC/FTC/TAF. The primary endpoint was HIV-1 RNA ≥50 copies/mL at Month 12 (Snapshot algorithm). Of 670 participants (modified intention-to-treat–exposed population), 20 were from Japan (LA, n = 14; BIC/FTC/TAF, n = 6). At Month 12, no participants in either Japanese group had HIV-1 RNA ≥50 copies/mL; 86 % (12/14; 2 participants withdrew) versus 100 % (6/6) had HIV-1 RNA &lt;50 copies/mL in the LA versus BIC/FTC/TAF groups; none had confirmed virologic failure. Withdrawals were due to a non–drug-related adverse event (AE; acute hepatitis B) and a physician decision. Excluding injection site reactions, drug-related AE rates were higher in the LA group (36 % vs 17 %; all grade 1 or 2). No drug-related serious AEs were reported. Injection site reactions were common (100 % [13/13] of LA participants); all were grade 1 or 2; none led to withdrawal; median duration was 4 days. Mean treatment satisfaction scores improved from baseline to Month 12 in the LA versus BIC/FTC/TAF Japanese groups (+6.25 vs + 0.33 on a 66-point scale). Though limited, these data suggest switching to CAB+RPV LA from BIC/FTC/TAF was well tolerated in Japanese participants, with comparable efficacy and improved treatment satisfaction.</div><div>ClinicalTrials.gov; NCT04542070 (<span><span>https://www.clinicaltrials.gov/study/NCT04542070</span><svg><path></path></svg></span>).</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102734"},"PeriodicalIF":1.9,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial susceptibility surveillance of bacterial isolates recovered in Japan from odontogenic infections in 2018","authors":"Akihiro Kaneko ,&nbsp;Hiroshi Iwabuchi ,&nbsp;Junko Sato ,&nbsp;Tetsuya Matsumoto ,&nbsp;Hiroshi Kiyota ,&nbsp;Naoki Hasegawa ,&nbsp;Hideaki Hanaki ,&nbsp;Hiroyuki Naito ,&nbsp;Tomohiro Hamada ,&nbsp;Hiroshi Yamazaki ,&nbsp;Kazuto Hoshi ,&nbsp;Masanobu Abe ,&nbsp;Yasunobu Busujima ,&nbsp;Takatsugu Suzuki ,&nbsp;Dai Nakashima ,&nbsp;Kazumi Izawa ,&nbsp;Tohru Akashiba ,&nbsp;Masataka Uematsu ,&nbsp;Fumitaka Terasawa ,&nbsp;Takefumi Morihana ,&nbsp;Yumiko Obayashi","doi":"10.1016/j.jiac.2025.102731","DOIUrl":"10.1016/j.jiac.2025.102731","url":null,"abstract":"<div><h3>Background</h3><div>This study reports the findings of the second antimicrobial susceptibility surveillance study of isolates recovered from odontogenic infections in Japan.</div></div><div><h3>Methods</h3><div>A total of 164 odontogenic infections samples including periodontitis (group 1, n = 33), pericoronitis (group 2, n = 7), jaw inflammation (group 3, n = 83), and phlegmon in the jawbone area (group 4, n = 42) from 21 facilities were included in the study.</div></div><div><h3>Results</h3><div>Antimicrobial susceptibility testing was performed on 515 isolates, consisting of 120, 115, 173, 38, and 69 <em>Streptococcus</em> spp., anaerobic gram-positive cocci, <em>Prevotella</em> spp., <em>Porphyromonas</em> spp., and <em>Fusobacterium</em> spp. isolates, respectively. The ratio of susceptible bacteria to causative agents of odontogenic infections was determined in accordance with the guidelines of the Clinical and Laboratory Standards Institute. The susceptibility rates of <em>Streptococcus anginosus</em> were as follows: ampicillin, 98.6 %; cefcapene, ceftriaxone, meropenem, and doripenem, 100 %; clarithromycin and azithromycin, 71.2 %; and clindamycin, 87.7 %. The MIC90 value for ampicillin for <em>Prevotella</em> spp. was 32 μg/mL, with a susceptibility rate of 65.3 %. The MIC90 value for sulbactam/ampicillin was 2 μg/mL, with a susceptibility rate of 100 %.</div></div><div><h3>Conclusions</h3><div>The findings of the present study indicate that the resistance to the combination of penicillins and β-lactamase inhibitors, the first-line antimicrobial agents for odontogenic infections and oral infections caused by anaerobic flora, are low.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102731"},"PeriodicalIF":1.9,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disseminated cat-scratch disease during abatacept therapy for rheumatoid arthritis in an older patient: A case report and review of the literature","authors":"Hiroaki Saito ,&nbsp;Yoshinori Takahashi ,&nbsp;Shunsuke Tsuge ,&nbsp;Ryo Nishioka ,&nbsp;Takeshi Zoshima ,&nbsp;Kiyoaki Ito ,&nbsp;Ichiro Mizushima ,&nbsp;Mitsuhiro Kawano ,&nbsp;Hiroko Ikeda ,&nbsp;Tomoyoshi Komiya ,&nbsp;Yasunori Iwata","doi":"10.1016/j.jiac.2025.102732","DOIUrl":"10.1016/j.jiac.2025.102732","url":null,"abstract":"<div><div>Cat-scratch disease (CSD) is a zoonotic infection primarily caused by <em>Bartonella henselae</em> following cat scratches. It typically presents with localized skin symptoms and self-limiting lymphadenopathy; however, in some cases, it can progress to disseminated lesions, leading to severe complications. We report a case of CSD with multiorgan involvement in a 79-year-old woman undergoing abatacept treatment for rheumatoid arthritis. She presented with persistent fever and painful right axillary lymphadenopathy, accompanied by induration on the right hand caused by a cat scratch. Imaging studies revealed multiple enlarged lymph nodes as well as hepatosplenic and cervical spine lesions. Initially, the patient was clinically diagnosed with CSD and initiated on azithromycin monotherapy; however, her symptoms persisted. A right axillary lymph node biopsy was performed to re-evaluate the diagnosis. Histopathological analysis showed numerous necrotizing granulomas with multinucleated giant cells, and a polymerase chain reaction assay confirmed the presence of <em>B. henselae</em> in the lymph node, establishing the diagnosis of CSD with multiorgan involvement. Treatment was adjusted to an azithromycin and rifampicin combination, resulting in a gradual improvement in symptoms. CSD may present as a fever of unknown origin with extranodal involvement affecting organs, such as the liver, spleen, bones, and central nervous system, or causing infective endocarditis, complicating the diagnosis. In immunosuppressed patients receiving biological agents for rheumatic diseases, a comprehensive evaluation is crucial. This should include a detailed medical history, physical examination, and comprehensive histological and microbiological assessments to differentiate CSD from conditions such as tuberculosis and malignant lymphoma.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102732"},"PeriodicalIF":1.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of Prevotella species in anaerobic culture-negative multiple abscesses using next-generation sequencing","authors":"Maki Goto ,&nbsp;Kei Yamamoto ,&nbsp;Kazuhiro Horiba ,&nbsp;Masanori Hashino ,&nbsp;Yuta Kubono ,&nbsp;Norio Ohmagari","doi":"10.1016/j.jiac.2025.102733","DOIUrl":"10.1016/j.jiac.2025.102733","url":null,"abstract":"<div><div>Next-generation metagenomic sequencing (mNGS) provides a comprehensive analysis of DNA and RNA in samples. In this case report, pus specimens were collected from an 86-year-old male with multiple muscle, periarticular, and bone abscesses, and osteomyelitis, without prior antimicrobial therapy. Results of bacterial culture tests, including anaerobic and intensified cultures, were negative. Despite this, mNGS using shotgun sequencing revealed a significant number of DNA and RNA nucleic acid reads from <em>Prevotella</em> spp. Although the subspecies could not be identified, mNGS allowed the selection of an appropriate antimicrobial agent and suggested significant oral contamination as the portal of entry. In summary, mNGS helped identify the causative organism in a case without prior antimicrobial therapy wherein culture test results were negative.</div></div>","PeriodicalId":16103,"journal":{"name":"Journal of Infection and Chemotherapy","volume":"31 7","pages":"Article 102733"},"PeriodicalIF":1.9,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}