Journal of Infection and Public Health最新文献

{"title":"The burden of viral skin diseases in the Middle East and North Africa region, 1990–2021","authors":"Fatemeh Amiri ,&nbsp;Saeid Safiri ,&nbsp;Reza Aletaha ,&nbsp;Seyed Ehsan Mousavi ,&nbsp;Mark J.M. Sullman ,&nbsp;Yousef Houshyar ,&nbsp;Ali-Asghar Kolahi ,&nbsp;Shahnam Arshi","doi":"10.1016/j.jiph.2025.102784","DOIUrl":"10.1016/j.jiph.2025.102784","url":null,"abstract":"<div><h3>Objective</h3><div>To report the prevalence, incidence, and Years Lived with Disability (YLD) due to viral skin diseases (VSD) in the MENA region from 1990 to 2021, focusing on age, sex, and socio-demographic index (SDI) using GBD 2021 data.</div></div><div><h3>Methods</h3><div>Data on the burden of VSD were sourced from the Global Burden of Disease (GBD) 2021 study, covering 21 countries in the MENA region. The results included the counts and age-standardised rates per 100,000 population, along with the associated 95 % uncertainty intervals (UIs).</div></div><div><h3>Results</h3><div>In 2021, the MENA region exhibited an age-standardised prevalence rate of 1257.6 cases per 100,000 population for VSD and an incidence rate of 990.5 cases per 100,000. Prevalence rates peaked sharply in the 5–9 age group before declining with age for both sexes, indicating a higher burden in younger populations. A strong positive relationship existed between the YLD rate and SDI, suggesting an increased burden of VSD with higher SDIs.</div></div><div><h3>Conclusion</h3><div>This study highlights the increasing burden of viral skin diseases (VSD) in the MENA region, especially among younger populations, with males also experiencing a slightly higher burden than females. Given the influence of multiple factors, a comprehensive strategy focusing on data systems, healthcare delivery, and regional collaboration is vital. Addressing these areas will help mitigate VSD's impact and improve health outcomes.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 8","pages":"Article 102784"},"PeriodicalIF":4.7,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The basic reproduction number is a reliable indicator in infectious diseases, but has misleading meta-analysis","authors":"Masoud Mohammadi","doi":"10.1016/j.jiph.2025.102807","DOIUrl":"10.1016/j.jiph.2025.102807","url":null,"abstract":"","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102807"},"PeriodicalIF":4.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causes and consequences of the emergence of monkeypox as a global public health concern","authors":"Hasnat Ahmad Bilal,&nbsp;Muhammad Ahmad,&nbsp;Ishtiaq Ahmed,&nbsp;Muhammad Imran Arshad,&nbsp;Mohammed Hussen Bule","doi":"10.1016/j.jiph.2025.102805","DOIUrl":"10.1016/j.jiph.2025.102805","url":null,"abstract":"","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 8","pages":"Article 102805"},"PeriodicalIF":4.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143946730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global prevalence and dynamics of mecA and mecC genes in MRSA: Meta-meta-analysis, meta-regression, and temporal investigation","authors":"Adeiza Shuaibu Suleiman ,&nbsp;Prosun Bhattacharya ,&nbsp;Md. Aminul Islam","doi":"10.1016/j.jiph.2025.102802","DOIUrl":"10.1016/j.jiph.2025.102802","url":null,"abstract":"<div><div>This study aimed to conduct first robust meta-meta-analysis on <em>mecC</em> and <em>mecA</em>-positive MRSA strains, determining their overall prevalence and temporal trends. It encompassed 14 meta-analyses, involving 322,269 samples from 553 index studies. MRSA strains exhibited <em>mecA</em> / <em>mecC</em> positivity ranging from 0.90 % to 69.98 %. Varied AMSTAR and QUOROM scores were observed. <em>mecC</em>-positive MRSA prevalence was 2.41 % (95 % CI: 0.72–7.78 %). Human <em>mecA</em>-positive MRSA had 21.77 % prevalence (95 % CI: 9.08–43.67 %), animal samples had 7.08 % prevalence (95 % CI: 3.93–12.42 %), and worldwide prevalence was 14.58 % (95 % CI: 6.61–29.14 %). Temporal trends reveal fluctuations, <em>mecA</em> prevalence spiked to 52.68 % in 2012 and decreased to 7.11 % in 2022. For <em>mecC</em> gene, the year 2015 marked a low incidence of 0.41 % (95 % CI: 0.05–3.54 %). Overall, <em>mecA</em> gene carriage surpassed <em>mecC</em> in MRSA strains, especially in humans.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102802"},"PeriodicalIF":4.7,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic next-generation sequencing (mNGS) identified Clostridium perfringens infection presenting as acute hemolysis after surgery","authors":"Shifang Yu ,&nbsp;Tinghui Cao ,&nbsp;Zhijiang Xu ,&nbsp;Hongwei Zhou ,&nbsp;Qiang Li","doi":"10.1016/j.jiph.2025.102798","DOIUrl":"10.1016/j.jiph.2025.102798","url":null,"abstract":"<div><div><em>Clostridium perfringens (C. perfringens) septicaemia is a rare, but rapidly fatal infection, characterized by massive hemolysis. In nu</em>merous documented instances, the patient was not diagnosed in time due to the absence of typical clinical features. In order to enhance diagnostic accuracy, metagenomic next-generation sequencing (mNGS) has been adopted as a novel approach to pathogen identification.</div></div><div><h3>Case presentation</h3><div>A 67-year-old male who had undergone transcatheter arterial chemoembolization (TACE) surgery four days earlier presented with severe anemia, and laboratory investigations disclosed intravascular hemolysis. Swift and accurate identification was imperative, resulting in the confirmation of mNGS analysis, his medical history, clinical symptoms, physical signs, additional tests, and <em>C. perfringens</em> as the causative pathogen of hemolysis. After successful treatment with effective antibiotics, the patient recovered and was discharged from the hospital after 19 days.</div></div><div><h3>Conclusions</h3><div>mNGS achieves expedited diagnostic turnaround time through rapid pathogen identification, significantly surpassing conventional culture methods. This may enable the diagnosis of atypical cases of <em>C. perfringens infection, which can cause rapid systemic shock, renal failure, intravascular hemolysis, and even death.</em></div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 8","pages":"Article 102798"},"PeriodicalIF":4.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthcare-associated infection and antimicrobial use among residents of long-term care facilities in Italy: Preliminary results of a point prevalence survey","authors":"Lorenzo Brambilla,&nbsp;Lolita Azrumelashvili,&nbsp;Manfredi Grasso,&nbsp;Assunta Gabrielli,&nbsp;Donatella Lania,&nbsp;Andrea Labruto,&nbsp;Letizia Oreni,&nbsp;Roberto Pupillo,&nbsp;Maria Rosa Sbardellati Tommaso Petitti,&nbsp;Antonio Schirripa,&nbsp;Federica Tartarone,&nbsp;Luciana Bevilacqua","doi":"10.1016/j.jiph.2025.102796","DOIUrl":"10.1016/j.jiph.2025.102796","url":null,"abstract":"<div><h3>Background</h3><div>Healthcare-associated infections (HAIs) are infections acquired in any care setting, including long-term residential facilities, rehabilitation units, and nursing homes, and which were not clinically manifest nor incubating at the time of admission to the facility. HAIs are among the most frequent adverse events encountered in healthcare settings, often caused by multi-resistant microorganisms that can infect patients, healthcare workers, and visitors, causing morbidity and mortality. Surveillance activities such as prevalence studies represent an opportunity to plan preventive actions and improve quality of care in all care contexts.</div></div><div><h3>Methods</h3><div>A point prevalence survey was conducted in November 2023 among the 22 long-term care facilities (LTCFs) managed by the Don Carlo Gnocchi ONLUS Foundation. Data were collected on a single day in each LTCF, using a web-based data collection methodology developed for the European HALT-4 study. Data collection teams at each facility, including a total of 97 surveyors, completed a survey-questionnaire, following training on the data collection method.</div></div><div><h3>Results</h3><div>Among the 2746 included residents, the prevalence of residents with at least one HAI was 4.8 % (95 % confidence interval [CI] 4.0 %–5.7 %). HAI prevalence ranged from 1.1 % (95 % CI 0.5 %–1.7 %) in residential care settings to 12.1 % (95 % CI 8.0 %–16.2 %) in intensive rehabilitation units. The prevalence of antimicrobial use was 7.3 % (95 % CI 6.3 %–8.3 %), with prevalence of 2.0 % (95 % CI 1.2 %–2.7 %) for residential care residents, 10.2 % (95 % CI 8.5 %–11.9 %) for non-intensive rehabilitation, and 20.2 % (95 % CI 15.2 %–25.2 %) for intensive rehabilitation.</div></div><div><h3>Conclusions</h3><div>This initial study allowed the assessment of the feasibility of conducting nationwide studies in various centers, and deepened our understanding of different care settings, as well as the clinical characteristics and complexity of hospitalized patients and residents in LTCFs.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102796"},"PeriodicalIF":4.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a multiplex genomic point-of-care strategy for rapid support of older people with respiratory infection in a geriatric hospital over a full winter season","authors":"Marion Fayolle ,&nbsp;Ludovic Lafaie ,&nbsp;Thomas Franck ,&nbsp;Marie Blanquet ,&nbsp;Fabien de Oliveira ,&nbsp;Thomas Bourlet ,&nbsp;Bruno Pozzetto ,&nbsp;Sylvie Pillet ,&nbsp;Thomas Célarier","doi":"10.1016/j.jiph.2025.102795","DOIUrl":"10.1016/j.jiph.2025.102795","url":null,"abstract":"<div><h3>Background</h3><div>In older patients, it is important to rapidly identify those infected by a significant pathogen in order to implement isolation measures and prescribe appropriate specific treatments when available and indicated. The objective of this study was to evaluate a multiplex genomic point-of-care (POC) strategy detecting four viruses, SARS-CoV-2, influenza virus (IV) A and B and respiratory syncytial virus (RSV), in a geriatric setting located 7 km from the central laboratory of the hospital.</div></div><div><h3>Methods</h3><div>This prospective monocentric study was conducted during the 2022–2023 winter season in two geriatric wards of the University Hospital of Saint-Etienne, France. Two automated quantitative reverse transcription polymerase chain reaction (RT-qPCR) tests were compared, the reference test at the central laboratory and the POC test performed by nurses. The main outcome was the saved time to result (TTR) by the clinician with the POC technology. Additional objectives included testing performance, health-economic considerations and healthcare workers’ acceptability.</div></div><div><h3>Results</h3><div>From 69 included infectious episodes, the reference test identified 18 viral infections (7 for SARS-CoV-2, 8 for RSV, 2 for both viruses, 1 for IVA). The POC test yielded similar performance with reference test (overall concordance of 98.55 % for SARS-CoV-2 and RSV). The POC strategy showed a gain of 10.43 ( ± 6.92) hours on TTR and a cumulative time of isolation of 488.27 hours in case of negative result. Further results showed economic savings and a strong acceptability by healthcare workers in favor of the POC strategy.</div></div><div><h3>Conclusions</h3><div>This pilot study illustrates the benefits of a genomic POC test to identify current respiratory viral infections in older people within a geriatric setting remote from the central laboratory during a winter season with multiple viral outbreaks.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102795"},"PeriodicalIF":4.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diminishing reactogenicity with preserved immunogenicity in COVID-19 vaccines: A longitudinal observation from primary to updated booster vaccine cohorts","authors":"Hyun Myung Kang ,&nbsp;Ho Jun Lee ,&nbsp;Jin Yang Baek ,&nbsp;Hye-Jin Kim ,&nbsp;Young Jae Lee ,&nbsp;Ju-Yeon Choi ,&nbsp;Hye-Sook Jeong ,&nbsp;Eui Ho Kim ,&nbsp;Kyong Ran Peck ,&nbsp;Jae-Hoon Ko","doi":"10.1016/j.jiph.2025.102794","DOIUrl":"10.1016/j.jiph.2025.102794","url":null,"abstract":"<div><h3>Background</h3><div>Encouraging annual updated COVID-19 vaccinations for high-risk populations is crucial for public health. However, concerns about significant reactogenicity persist, contributing to vaccine hesitancy. To investigate evolving COVID-19 vaccine reactogenicity and immunogenicity, we conducted a longitudinal analysis across three COVID-19 vaccine cohorts.</div></div><div><h3>Methods</h3><div>The Primary Vaccine Cohort, receiving wild-type (WT) 1st to 3rd doses and WT-BA.4/5 bivalent vaccine; the XBB.1.5 Monovalent Vaccine (MoV) Cohort; and the ongoing JN.1 MoV Cohort were investigated. Reactogenicity was assessed using electronic diaries for eight days, and serological responses were measured through quantitative anti-spike protein antibody (Sab) assay. Plaque reduction neutralization testing (PRNT) was performed against WT SARS-CoV-2 and vaccine-specific variants.</div></div><div><h3>Results</h3><div>A total of 290 participants with 690 vaccine doses and 1222 sampling points was included. Total symptom scores decreased serially from the WT 1st dose to JN.1 MoV, with changes pronounced in the younger age group (&lt; 45 years; Spearman r = -0.13, <em>P</em> = 0.008). Changes were not evident in the older age group (≥ 45 years) with consistently low reactogenicity. Severe reactions also steadily declined from 26.2 % (WT 1st) to 3.3 % (JN.1 MoV). Serological analysis revealed plateauing post-vaccination Sab titers with increasing pre-vaccination levels and robust PRNT responses against vaccine strains. Age negatively correlated with Sab levels after the 1st WT dose but not in subsequent doses. Multivariable analysis found no significant association between reactogenicity and immunogenicity.</div></div><div><h3>Conclusion</h3><div>The observed decline in reactogenicity, alongside sustained immunological responses, supports the safety and efficacy of annual COVID-19 booster vaccination programs.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102794"},"PeriodicalIF":4.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical features and antibiotic resistance in pediatric pneumococcal meningitis in Southern Vietnam, 2012–2023: A multicenter retrospective study","authors":"Hieu Cong Truong ,&nbsp;Thanh Van Phan ,&nbsp;Hung Thanh Nguyen ,&nbsp;Thang Vinh Ho ,&nbsp;Dai Thi Trang Vo ,&nbsp;Thuong Vu Nguyen ,&nbsp;Trung Vu Nguyen ,&nbsp;Niko Speybroeck","doi":"10.1016/j.jiph.2025.102797","DOIUrl":"10.1016/j.jiph.2025.102797","url":null,"abstract":"<div><h3>Background</h3><div><em>Streptococcus pneumoniae</em> is a major cause of morbidity and mortality in children. This study investigates the clinical features, cerebrospinal fluid (CSF) findings, serotype distribution, and antibiotic resistance patterns in Vietnamese children aged 1–59 months with pneumococcal meningitis (PM).</div></div><div><h3>Methods</h3><div>This retrospective study (2012–2023) was conducted at two tertiary pediatric hospitals in Ho Chi Minh City. CSF samples from probable bacterial meningitis (PBM) cases were analyzed using biochemistry, culture, and real-time polymerase chain reaction (rt-PCR). Serotyping and antibiotic-resistance genes were identified using quadriplex rt-PCR.</div></div><div><h3>Results</h3><div>Among 2922 PBM cases, 155 (5.3 %) were confirmed as PM. Of these, 58.7 % occurred in children under one year and 62.6 % during the rainy season. Fever (98.1 %) and vomiting (67.7 %) were the most common symptoms. Infants under 12 months frequently exhibited nonspecific signs like convulsions (48.4 %) and bulging fontanels (34.1 %), while older children displayed classic symptoms such as neck stiffness (32.4 %) and behavioral changes (26.5 %). CSF analysis revealed turbid appearance, WBC ≥ 100 cells/mm³ (85.2 %), and protein ≥ 1 g/L (60.0 %) (p &lt; 0.05). Severe outcomes were noted in 17.4 % of PM cases, with a 3.2 % fatality rate. The 13-valent pneumococcal conjugate vaccine (PCV13) serotypes caused 81.3 % of confirmed cases, predominantly serotypes 6 A/B (34.8 %) and 19 F (20.0 %). Among 137 isolates tested, high prevalence rates were observed for the <em>pbp2b</em> (68.6 %), <em>mef(A)/erm(B)</em> (93.4 %), and <em>tetM</em> (92.0 %) resistance genes. Additionally, 61.3 % of isolates showed multiple resistance genes, particularly in serotypes 6 A/B, 23 F, 9 V, and 13. Antibiotic resistance in non-PCV13 serotypes increased over time.</div></div><div><h3>Conclusions</h3><div>PM in Vietnamese children presents age-specific clinical presentations and is predominantly caused by highly resistant PCV13 serotypes. The rising resistance in non-PCV13 serotypes poses a formidable challenge in managing pneumococcal infections. These findings emphasize the urgent need for PCV introduction in the national immunization program and ongoing resistance surveillance.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102797"},"PeriodicalIF":4.7,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular surveillance of bat flies (Diptera: Nycteribiidae and Streblidae) and other ectoparasites in Ratchaburi, Thailand: Unraveling host associations and coronavirus transmission dynamics in the context of zoonotic spillover risk","authors":"Atchara Phumee ,&nbsp;Khwankamon Rattanatumhi ,&nbsp;Opass Putcharoen ,&nbsp;Suwalak Chitcharoen ,&nbsp;Nataya Sutthanont ,&nbsp;Charuai Suwanbamrung ,&nbsp;Thanapat Pataradool ,&nbsp;Rungfar Boonserm ,&nbsp;Sakone Sunantaraporn ,&nbsp;Chatuthanai Savigamin ,&nbsp;Jonas Schmidt-Chanasit ,&nbsp;Padet Siriyasatien","doi":"10.1016/j.jiph.2025.102792","DOIUrl":"10.1016/j.jiph.2025.102792","url":null,"abstract":"<div><h3>Background</h3><div>Bats act as hosts for various ectoparasites, including bat flies, bugs, fleas, ticks, and mites, which play crucial roles in the transmission of bat-borne pathogens. As obligate blood-feeding parasites, these ectoparasites can serve as direct vectors or indirectly influence pathogen dynamics in bat populations. In Thailand, molecular studies on bat ectoparasites are limited, with scarce data on their diversity, distribution, and role in zoonotic pathogen transmission. This study aims to provide the first molecular characterization of bat flies and other ectoparasites in Ratchaburi, Thailand, focusing on their blood meals and potential involvement in coronavirus transmission.</div></div><div><h3>Methods</h3><div>Ectoparasites were identified using the cytochrome oxidase I (<em>COI</em>) gene, while host blood meals were confirmed using the cytochrome b (<em>CytB</em>) gene. A total of 37 bat flies and additional ectoparasites (ticks and flea) were analyzed. Coronavirus screening was conducted through targeted amplification of the RNA-dependent RNA polymerase (<em>RdRp</em>) gene, followed by sequencing and phylogenetic analysis of positive samples.</div></div><div><h3>Results</h3><div>Three bat fly genera were identified: <em>Nycteribia</em> sp. (20), <em>Phthiridium</em> sp. (15), and <em>Raymondia</em> sp. (2). Blood meal analysis indicated host associations with <em>Rhinolophus coelophyllus</em> for <em>Nycteribia</em> sp., <em>R. pusillus</em> and <em>Chaerephon plicatus</em> for <em>Phthiridium</em> sp., and <em>R. pusillus</em> for <em>Raymondia</em> sp. Additionally, two <em>Ixodes</em> sp. ticks and one <em>Hystrichopsylla</em> sp. flea were found, all associated with <em>C. plicatus</em>. Coronavirus screening identified positive samples, with sequencing revealing alphacoronaviruses and betacoronaviruses.</div></div><div><h3>Conclusions</h3><div>This study highlights the importance of molecular tools in characterizing ectoparasites, their blood meal sources, and associated pathogens. It underscores the potential of ectoparasites as non-invasive tools for coronavirus detection in bats, addressing knowledge gaps and contributing to public health strategies for mitigating zoonotic spillover risks.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 7","pages":"Article 102792"},"PeriodicalIF":4.7,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}