Journal of Infection and Public Health最新文献

{"title":"Burkholderia cepacia complex infections beyond hospital outbreaks: A 22-Year analysis of clinical characteristics, antimicrobial resistance, and mortality predictors","authors":"Marwan Jabr Alwazzeh ,&nbsp;Amani Alnimr ,&nbsp;Sara M. Alwarthan ,&nbsp;Mashael Alhajri ,&nbsp;Jumanah Algazaq ,&nbsp;Bashayer M. AlShehail ,&nbsp;Basel A. Darweesh ,&nbsp;Mohammed A. Basheikh ,&nbsp;Reem S. AlSulaiman ,&nbsp;Saud A. Alamro ,&nbsp;Jawad ur Rahman ,&nbsp;Mohammad T. Al-Hariri","doi":"10.1016/j.jiph.2026.103158","DOIUrl":"10.1016/j.jiph.2026.103158","url":null,"abstract":"<div><h3>Background</h3><div><em>Burkholderia cepacia</em> complex bacteria are intrinsically multidrug-resistant opportunistic pathogens that pose a growing threat in hospital settings, particularly among immunocompromised patients. Despite its clinical significance, data on sporadic <em>Burkholderia cepacia</em> complex infections outside cystic fibrosis populations and hospital outbreaks remain limited.</div></div><div><h3>Methods</h3><div>A 22-year retrospective observational cohort study (May 2001–April 2023) was carried out at a tertiary care academic center in the Eastern Province of Saudi Arabia. Adults hospitalized with confirmed <em>Burkholderia cepacia</em> infection and ≥ 72 h of antibiotic therapy were included. Demographics, comorbidities, infection type, microbiological resistance profiles, treatment outcomes, and mortality predictors were analyzed using logistic regression and Kaplan-Meier survival estimates.</div></div><div><h3>Results</h3><div>Of 189 identified cases, 151 patients met the inclusion criteria (mean age 53 ± 21 years; 57.6 % male). The majority of infections were hospital-acquired (84.8 %), with bloodstream infections (33.1 %) and ventilator-associated pneumonia (26.5 %) being the most prevalent. Resistance was high to piperacillin/tazobactam (73.6 %), ceftazidime (58.6 %), levofloxacin (57.4 %), carbapenems (42.0 %), and trimethoprim-sulfamethoxazole (30.3 %). Clinical resolution was achieved in 82.1 %, but bacteriological eradication occurred in only 56.9 %, with a recurrence rate of 17.2 %. Mortality reached 9.9 % at 14 days, 17.2 % at 30 days, and 35.8 % at one year. Independent predictors of 30-day mortality included advanced age, critical care admission, central venous catheter insertion, mechanical ventilation, and resistance to fluoroquinolones or carbapenems.</div></div><div><h3>Conclusions</h3><div>Our findings indicate that <em>Burkholderia cepacia</em> complex infections represent a significant clinical challenge due to antibiotic resistance and elevated mortality rates. The data highlight the importance of improving diagnostic and treatment approaches, as well as implementing effective infection control policies, especially for vulnerable hospitalized populations.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103158"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics profiles associated with SARS-CoV-2 -IgG serostatus as an alternative diagnostic approach","authors":"Mariam M. Al Eissa ,&nbsp;Reem H. AlMalki ,&nbsp;Randh Alahmari ,&nbsp;Raghad A. AlQurashi ,&nbsp;Esraa A. Hawsa ,&nbsp;Muath ben shaded ,&nbsp;Monera Alrukhayes ,&nbsp;Ahdab A. Alsaieedi ,&nbsp;Yousef Mall ,&nbsp;Afshan Masood ,&nbsp;Sami S. Almudrra ,&nbsp;Khaled I. AlAbdulkareem ,&nbsp;Khalid H. AlAnazi ,&nbsp;Assim A. Alfadda ,&nbsp;Ahmed AlBarraq ,&nbsp;Abdullah M. Asiri ,&nbsp;Hani A. Jokhdar ,&nbsp;Anas Abdel Rahman","doi":"10.1016/j.jiph.2026.103155","DOIUrl":"10.1016/j.jiph.2026.103155","url":null,"abstract":"<div><h3>Background</h3><div>The coronavirus disease 2019 (COVID-19) pandemic, caused by SARS-CoV-2, showed a wide range of host responses from no symptoms to severe illness, highlighting the urgent need for better diagnostic and therapeutic solutions. Untargeted metabolomics offers a way to uncover molecular and biochemical changes linked to immune differences, potentially identifying biomarkers for early detection and treatment monitoring.</div></div><div><h3>Method</h3><div>This cross-sectional observational study recruited 49 individuals. Samples were stratified using the SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) into two groups SARS-CoV-2 IgG-negative (n = 26) and SARS-CoV-2 IgG-positive (n = 23). Samples were analyzed using LC/MS-based untargeted metabolomics. The data were processed using a standard pipeline that included multivariate statistical analysis, molecular annotation, pathway and network analyses.</div></div><div><h3>Results</h3><div>Metabolomic analysis of SARS-CoV-2 IgG-positive and SARS-CoV-2 IgG-negative groups revealed 319 dysregulated metabolites, comprising 218 up- and 101 down-regulated metabolites. ROC analysis revealed the metabolites 4-hydroxysphinganine and Ganglioside GM1 (18:1/22:0), with the highest AUC of 0.883 and 0.95, respectively. The significantly affected metabolic pathways identified to be dysregulated between the two groups belonged to ubiquinone, tyrosine, pyrimidine, and glutathione metabolism. Network pathway analysis demonstrated that the identified metabolites together were involved in regulating lipid metabolism, small molecule biochemistry, and cell-cell signaling and interaction, and were centered around dysregulation of TNF, IL-23, JNK, IL-1β, and CRP signaling pathways. The top metabolites identified in the interaction network pathway were L-Fucose, phosphorylcholine, cerotic acid, 5-hydroxydecanoic acid, and paired immunoglobulin-like type 2 receptor beta (PILRB).</div></div><div><h3>Conclusion</h3><div>Our findings identify metabolomic alterations associated with SARS-CoV-2 infection between IgG-positive and IgG-negative individuals. The identified metabolites are involved in regulating lipid metabolism, immune response, and the inflammatory signaling pathways. The results offer preliminary hypothesis generating insights into the underlying changes that may inform future research on host–immune interactions in COVID-19. Changes in these metabolites need to be validated in larger and independent cohorts.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103155"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146227275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital twin of Mycobacterium tuberculosis infection: Integrating immune dynamics and pathogen adaptation for precision therapy","authors":"Ruqaiyyah Siddiqui ,&nbsp;Naveed Ahmed Khan","doi":"10.1016/j.jiph.2026.103154","DOIUrl":"10.1016/j.jiph.2026.103154","url":null,"abstract":"<div><div><em>Mycobacterium tuberculosis</em> remains one of the world’s most persistent pathogens, responsible for over a million deaths each year despite effective drugs and decades of research. Disease progression varies widely among individuals, reflecting interactions between pathogen, physiology, host immunity, and pharmacological response. We propose a digital twin framework for tuberculosis that integrates clinical, immunological, and pharmacokinetic data into a continuously adaptive computational model. The twin would simulate host-pathogen dynamics from granuloma formation to systemic immune regulation, linking these processes with individualised drug exposure and treatment response. By forecasting outcomes and identifying early indicators of relapse or resistance, such a system could guide precision therapy and accelerate discovery of host-directed interventions. The tuberculosis digital twin thus represents a bridge between infection biology, computation, and clinical translation, presenting an evolving model capable of transforming how this ancient disease is understood and managed. However, translation will require further longitudinal clinical and immunological datasets and systematic validation of model predictions in real-world treatment settings.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103154"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Circular genome of human bocavirus 1 associated with high load of viral DNA, positive antigen and increased risk of severe pneumonia in children","authors":"Kexiang Zhang ,&nbsp;Ri De ,&nbsp;Zeng Li ,&nbsp;Yanpeng Xu ,&nbsp;Zhenzhi Han ,&nbsp;Runan Zhu ,&nbsp;Yu Sun ,&nbsp;Liping Jia ,&nbsp;Dongmei Chen ,&nbsp;Yutong Zhou ,&nbsp;Qi Guo ,&nbsp;Yao Yao ,&nbsp;Xiaolin Ma ,&nbsp;Shuang Liu ,&nbsp;Chunmei Zhu ,&nbsp;Dong Qu ,&nbsp;Linqing Zhao","doi":"10.1016/j.jiph.2026.103156","DOIUrl":"10.1016/j.jiph.2026.103156","url":null,"abstract":"<div><h3>Background</h3><div>Human bocavirus 1 (HBoV1) causes acute respiratory infections (ARIs) in children, but its diagnosis is complicated by prolonged viral shedding. There are indications that the detection of a circular genome in a clinical specimens may be associated with acute infection.</div></div><div><h3>Methods</h3><div>Respiratory specimens collected from pediatric patients with ARIs during January 2021 to July 2024 were screened by a duplex qPCR, which was developed to distinguish circular genome from total viral genomes and evaluated by nested PCR and antigen test. Clinical data were collected from patients with single HBoV1 infection to reveal the association of circular genome with ARIs and the severity of pneumonia.</div></div><div><h3>Results</h3><div>Among 520 specimens positive for HBoV1 DNA, 206 (39.61 %) were positive for circular genomes as determined by duplex qPCR, with the median load of total genomes 10^10.08 (IQR 10^9.26, 10^10.62) copies/mL significantly higher than 10^7.81 (IQR 10^6.88, 10^8.60) copies/mL in the circular genome negative group (<em>p</em> &lt; 0.0001). In the antigen-positive group, the positive rate for circular genomes was 78.57 % (44/56), significantly higher than 34.29 % (108/315) observed in the antigen-negative group. Among patients single positive for HBoV1, the circular genome-positive group (n = 106) showed more severe clinical manifestations and required more intensive treatment. Logistic regression analysis identified the circular genome as a strong independent risk factor for severe pneumonia (OR = 6.38, AUC = 0.82).</div></div><div><h3>Conclusion</h3><div>Circular genome of HBoV1 associated with high load of viral DNA, positive antigen and severe pneumonia in children may serve as a biomarker for acute HBoV1 infection and severe pneumonia.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103156"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146102488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypervirulent and carbapenem-resistant Klebsiella pneumoniae: A call for global surveillance and coordinated action","authors":"Shital Ghogale,&nbsp;Ketaki Pathak","doi":"10.1016/j.jiph.2026.103141","DOIUrl":"10.1016/j.jiph.2026.103141","url":null,"abstract":"","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103141"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146125319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Empowering active surveillance of Carbapenem-resistant Enterobacterales: Insights from one year of genomic surveillance","authors":"Greta Petazzoni ,&nbsp;Stefano Gaiarsa ,&nbsp;Irene Mileto ,&nbsp;Angela Kuka ,&nbsp;Marta Corbella ,&nbsp;Cristina Merla ,&nbsp;Antonio Piralla ,&nbsp;Marina Ramus ,&nbsp;Aurora Piazza ,&nbsp;Vittoria Mattioni Marchetti ,&nbsp;Roberta Migliavacca ,&nbsp;Alba Muzzi ,&nbsp;Patrizia Cambieri ,&nbsp;Fausto Baldanti","doi":"10.1016/j.jiph.2026.103187","DOIUrl":"10.1016/j.jiph.2026.103187","url":null,"abstract":"<div><h3>Background</h3><div>Healthcare-associated infections (HAIs) in Europe affect millions of patients annually, driving antimicrobial resistance and imposing significant economic burdens. Carbapenem-resistant <em>Enterobacterales</em> (CRE) are particularly concerning due to their ability to spread, making genomic surveillance essential for tracking their transmission.</div></div><div><h3>Methods</h3><div>From October 2022 to September 2023, we sequenced and analysed the genome of 340 CRE obtained from patients admitted to Fondazione IRCCS Policlinico San Matteo ( Italy). Sequencing was performed using a short-read approach and genomes were <em>in-silico</em> characterized to identify Sequence Types (STs), antimicrobial resistance and virulence determinants, and plasmid content. Moreover, a core SNP-based phylogeny was performed to identify potential epidemic clusters within the same clone.</div></div><div><h3>Results</h3><div>Among the 340 CRE isolated from surveillance (N = 234) and other clinical specimens (N = 106), 87.7% belonged to the <em>Klebsiella</em> genus, primarily <em>K. pneumoniae</em> (CR-Kp, N = 284). <em>Escherichia coli</em> (CR-Ec) accounted for 8.5% (N = 29); while the remaining species were <em>Enterobacter spp.</em> (N = 13) and <em>Citrobacter spp.</em> (N = 9). <em>bla</em>_KPC and <em>bla</em>_NDM gene variants were detected in 55.9% and 25.6% of CRE, respectively. The most prevalent CR-Kp clones were ST307, ST6668, ST258, and ST512, whereas ST131 was dominant among CR-Ec. Large epidemic clusters (ECs; ≥ 5 strains in each clade) were mainly found in CR-Kp associated with ST307 and ST6668, with 80.63% of CR-Kp grouped into nine ECs. Conversely, other species were observed as sporadic cases or within transmission clusters with limited spread.</div></div><div><h3>Conclusion</h3><div>Our comprehensive analysis of CRE circulation at HSM revealed that their dissemination was largely driven by highly successful clones, shedding light on several of them.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103187"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole blood gene expression to assess disease severity of omicron-associated acute encephalitis and croup in children: A transcriptomic and immune profiling study","authors":"Chong-Wei Huang ,&nbsp;Wei-Chao Liao ,&nbsp;Ian Yi-Feng Chang ,&nbsp;En-Wei Hsing ,&nbsp;Chung-Guei Huang ,&nbsp;Jainn-Jim Lin ,&nbsp;Kuang-Lin Lin ,&nbsp;Cheng-Hsun Chiu ,&nbsp;Chih-Ho Chen ,&nbsp;Jen-Han Chen ,&nbsp;Wen-I Lee ,&nbsp;Yu-Chia Hsieh","doi":"10.1016/j.jiph.2026.103181","DOIUrl":"10.1016/j.jiph.2026.103181","url":null,"abstract":"<div><h3>Background</h3><div>The Omicron variant of SARS-CoV-2 has caused severe complications in children, including encephalitis/encephalopathy and croup, yet the underlying pathogenic mechanisms remain unclear. Transcriptome analysis could provide insights into immune responses and guide clinical management strategies.</div></div><div><h3>Methods</h3><div>We conducted a case-control cohort study of COVID-19 patients across two medical centers in Taiwan from April 17 to November 7, 2022. Blood RNA sequencing was used to analyze differences in gene expression and functional pathways between cases of encephalitis/encephalopathy or croup of varying severity and controls with mild febrile disease. We applied computational analyses to identify transcriptomic signatures linked to COVID-19 associated encephalitis/encephalopathy.</div></div><div><h3>Results</h3><div>This study included 61 patients diagnosed with SARS-CoV-2 infection: 29 with mild febrile disease, 14 with croup, and 18 with encephalitis/encephalopathy. More differentially expressed genes were identified in encephalitis/encephalopathy (n = 834) than in croup (n = 185). Functional enrichment analysis revealed coordinated upregulation of myeloid leukocyte, neutrophil, and macrophage pathways in encephalitis/encephalopathy, accompanied by downregulation of HLA class II expression, antigen presentation, and T-cell activation, with changes tracking disease severity. CIBERSORT analysis revealed increased activated dendritic cells and reduced naïve CD4 T cells, CD8 T cells, naïve B cells, and NK cells. Croup patients showed reduced antigen presentation and low T cell receptor signaling. Gene expression profiles differentiated encephalitis/encephalopathy from mild febrile disease with a positive predictive value of 95.3 %.</div></div><div><h3>Conclusion</h3><div>Future interventions aiming at innate immune dysregulation, such as neutrophil inhibition, addressing macrophage activation, and enhancing T-cell responses, could play a promising role in improving outcomes for children infected with emerging, virulent SARS-CoV-2 variants.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103181"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of resistome profiles in ocular pathogens: Insights into Pseudomonas aeruginosa and emerging resistance trends","authors":"Raghav Krishnan Kulandaivelu ,&nbsp;Aditi Roy ,&nbsp;Soham Roy ,&nbsp;J.S. Amith Velavan ,&nbsp;Reddysai Amudala ,&nbsp;Debraj Joel Das Adhikari ,&nbsp;Karan S.V. ,&nbsp;Sudha Ramaiah ,&nbsp;Anand Anbarasu","doi":"10.1016/j.jiph.2026.103183","DOIUrl":"10.1016/j.jiph.2026.103183","url":null,"abstract":"<div><div>Eye infections are a significant area of microbial adaptation and therapeutic challenge, as antibiotic resistance threatens vision. In the last ten years, our knowledge of the ocular resistome has expanded through genomics and molecular epidemiology. An analysis of global evidence from 2015 to 2025 on the resistance architecture of major ocular pathogens will be presented, with a focus on <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa)</em>. The organism shows remarkable genomic flexibility by incorporating intrinsic resistance elements, biofilm-mediated tolerance, and by acquiring ARGs such as <em>bla</em>_VIM, <em>bla</em>_GES, QnrVC and RmtB by horizontal transfer. According to researchers, <em>Staphylococcus aureus</em> (<em>S. aureus</em>)<em>, Streptococcus pneumoniae</em> (<em>S. pneumoniae)</em>, and <em>Acinetobacter baumannii (A. baumannii)</em> have evolved mechanisms of drug resistance. Geographic analysis shows regional disparities, particularly in South Asia and North America, which are associated with antibiotic use and clinical exposure. To prevent the emergence of ocular antimicrobial resistance, there’s an urgent need for rapid molecular diagnostics, genomic-based surveillance, and responsible antimicrobial stewardship.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103183"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calls for papers: Antimicrobial resistance and one health: interdisciplinary strategies to address a global challenge – Virtual special issue","authors":"","doi":"10.1016/j.jiph.2025.103122","DOIUrl":"10.1016/j.jiph.2025.103122","url":null,"abstract":"","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103122"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Community-driven policy recommendations for dengue prevention and control in Thailand: A mixed-methods study","authors":"Chawarat Rotejanaprasert ,&nbsp;Ngamphol Soonthornworasiri ,&nbsp;Lokachet Tanasugarn ,&nbsp;Amorn Leelarasamee ,&nbsp;Kulkanya Chokephaibulkit ,&nbsp;Udomsak Narkkul ,&nbsp;Peeradone Srichan ,&nbsp;Khuanchai Koompapong ,&nbsp;Piroon Mootsikapun ,&nbsp;Winai Ratanasuwan ,&nbsp;Saranath Lawpoolsri","doi":"10.1016/j.jiph.2026.103166","DOIUrl":"10.1016/j.jiph.2026.103166","url":null,"abstract":"<div><h3>Background</h3><div>The World Health Organization roadmap targets the eradication of dengue, a neglected tropical disease. Community participation is crucial for successful control efforts, which must transcend technical solutions to engage and motivate the public. This study examined the needs, perceptions, and emotional drivers underpinning dengue prevention efforts and recommended tailored policies to control dengue in varied settings.</div></div><div><h3>Methods</h3><div>This mixed-methods study, using a triangulation design, was conducted in four geographical regions of Thailand between November 2023 and April 2024. Quantitative surveys were administered to 664 community members and 430 public health personnel, complemented by 10 focus group discussions (FGDs) with community members, 16 FGDs, and 23 in-depth interviews (IDIs) with public health personnel. Logistic regression was used to examine factors associated with dengue control practices, while qualitative data were analyzed thematically. Findings from both components were integrated during interpretation to enhance the robustness of conclusions.</div></div><div><h3>Results</h3><div>Integrated quantitative and qualitative findings showed that most participants practiced active dengue prevention. Social support strongly influenced preventive behaviors (OR 19.81; 95 % CI 8.53–46.03), whereas demographic factors and perceived susceptibility or severity were not significant. Higher practice levels were observed among participants in the northeast and health personnel aged ≥ 50 years. Qualitative data reinforced these findings, emphasizing the vital role of village health volunteers and challenges such as limited participation, low risk perception, poor sanitation, and cultural barriers shaping vaccine hesitancy.</div></div><div><h3>Conclusion</h3><div>Effective dengue control requires context-specific, evidence-based strategies that strengthen community participation, empower village health volunteers, and enhance vector control. Early warning systems and intersectoral collaboration are vital in high-risk areas, while transparent, culturally tailored communication can improve vaccine acceptance. These findings provide evidence to guide policy toward sustainable, community-centered dengue prevention and control.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"19 4","pages":"Article 103166"},"PeriodicalIF":4.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}