Zuohui Wang , Qingyan Wang , Jiaqi Li , Wenqian Huang
{"title":"Non-destructive detection of soluble solids content in Shawo radish with spatial spectra extraction method based on the full transmission near-infrared spectroscopy","authors":"Zuohui Wang , Qingyan Wang , Jiaqi Li , Wenqian Huang","doi":"10.1016/j.jfca.2025.108364","DOIUrl":"10.1016/j.jfca.2025.108364","url":null,"abstract":"<div><div>Shawo radish valued for its unique flavor and nutritional properties, requires rapid and non-destructive soluble solids content (SSC) assessment for quality control. The inherent variability in Shawo radish length and heterogeneous internal compositional distribution hinder accurate prediction of both full-region and region-specific SSC using conventional spectroscopic techniques. This study developed a novel analytical approach based on near-infrared spectroscopy. Utilizing a self-developed short-integration full-transmission online device for dynamic multi-point spectral acquisition, a preprocessing method with spatial spectra extraction was introduced. This method minimized the interference from the regions with low SSC-correlation while enabling target spectral captured from specific regions. The effect of method was validated by comparing the models built with complete and extracted spectra. Optimization determined that extracting [5 %,15 %] of the total numbers of the spectra, combined with Savitzky-Golay Smoothing plus Standard Normal Variate (SGS+SNV), Competitive Adaptive Reweighted Sampling (CARS), and Partial Least Squares Regression (PLSR), yielded the optimal model for predicting the full-region SSC (<em>R</em><sub><em>p</em></sub>=0.894, <span><math><mi>RMSEP</mi></math></span>=0.386°Birx, <em>RDP</em>=2.209). Furthermore, spatial spectra extraction successfully predicted SSC in the critical middle section of the radish (<em>R</em><sub><em>p</em></sub>=0.826, <span><math><mi>RMSEP</mi></math></span>=0.520°Brix, <em>RDP</em>=1.680). These results demonstrate the spatial spectra extraction significantly enhances the accuracy of online dynamic SSC prediction for both full-region and region-specific quality assessment in Shawo radish.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108364"},"PeriodicalIF":4.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhe Wang , Zixiao Wang , Jiamin Wang , Yu He , Junping Wang
{"title":"Novel electrochemical sensor with chiral recognition function prepared from N-CDs and cobalt-based nanomaterials for the detection of tyrosine enantiomers in food","authors":"Zhe Wang , Zixiao Wang , Jiamin Wang , Yu He , Junping Wang","doi":"10.1016/j.jfca.2025.108353","DOIUrl":"10.1016/j.jfca.2025.108353","url":null,"abstract":"<div><div>A novel chiral electrochemical sensor was fabricated via the assembly of chiral nitrogen-doped carbon dots (N-CDs) with cobalt-based nanomaterials (Co@CoO) for the rapid discrimination and quantification of tyrosine enantiomers (L/D-Tyr). The sensor has a wide linear range of 1–120 µM and low detection limits of 0.64 µM for L-Tyr and 0.3 µM for D-Tyr. The synergistic interactions between N-CDs and Co@CoO amplify stereoselective recognition, yielding a chiral current ratio (ΔI = I<sub>L</sub>/I<sub>D</sub>) of 1.65, which outperforms those of previously reported sensors. The chiral recognition mechanism was elucidated using cyclic voltammetry, electrochemical impedance spectroscopy, X-ray photoelectron spectroscopy, circular dichroism spectroscopy, Zeta potential analysis, and molecular dynamics simulations. The sensor demonstrates exceptional selectivity, stability, reproducibility, and reusability performance, and was successfully applied to the detection of L/D-Tyr in skim milk and yogurt. Notably, this sensor is the first to identify abnormally elevated D-Tyr levels in expired food products, suggesting a potential correlation between amino acid racemization and food quality deterioration. This study establishes a robust platform for chiral recognition in complex food matrices.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108353"},"PeriodicalIF":4.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pallabe Saha , Nusrat Tabassum Shristy , Aynun Nahar , Paroma Arefin , Mohammad Moniruzzaman , A.H.M. Shofiul Islam Molla Jamal , Muhammad Abdullah Al-Mansur , Md. Ahedul Akbor
{"title":"Assessment of caffeine, trace metals, and associated health risks in popular carbonated and energy drinks in Bangladesh using advanced analytical techniques","authors":"Pallabe Saha , Nusrat Tabassum Shristy , Aynun Nahar , Paroma Arefin , Mohammad Moniruzzaman , A.H.M. Shofiul Islam Molla Jamal , Muhammad Abdullah Al-Mansur , Md. Ahedul Akbor","doi":"10.1016/j.jfca.2025.108344","DOIUrl":"10.1016/j.jfca.2025.108344","url":null,"abstract":"<div><div>In this study, the concentrations of caffeine and trace metals in 20 popular soft drinks (carbonated and energy drinks) brands consumed in Bangladesh were analyzed using high-performance liquid chromatography and inductively coupled plasma-mass spectrometry, respectively, to evaluate potential health risks associated with their consumption. Also, physicochemical parameters such as pH, total dissolved solids (TDS) and conductivity were determined. The levels of caffeine in soft drink samples varied from ND (<1 mg/L) to 150.9 mg/L with an average of 120.0 mg/L. The mean concentration (µg/L) of As, Se, Pb, Cd, Co, Cr, Cu, Mn, Ni, Zn, Hg, Be, V, Al and Fe detected in carbonated and energy drinks were (3, 2), (1, 1), (257, 249), (4, 4), (3, 2), (72, 26), (132, 63), (166, 61), (76, 52), (2330, 3195), (7, 5), (0.3, 0.2), (2, 2), (1520, 1437) and (5648, 2608), respectively. The pH, TDS and conductivity of the samples varied from 2.90 ± 0.05–4.80 ± 0.05, 275–1694 mg/L, and 361–3300 μS/cm. The levels of Pb, Hg and Fe exceeded the safe limit in both carbonated and energy drink samples. Target hazard quotient and total hazard quotient were found to be less than 1 for all soft drink samples, indicating tolerable risk for human health.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108344"},"PeriodicalIF":4.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yu-an Chen , Ju Chen , Fengjie Zou , Yong Chen , Xueya Wang , Guihua Peng , Yong Yin , Jia Yan
{"title":"A novel manifold discriminant extreme learning machine combined with an E-nose for chili pepper identification via aroma analysis","authors":"Yu-an Chen , Ju Chen , Fengjie Zou , Yong Chen , Xueya Wang , Guihua Peng , Yong Yin , Jia Yan","doi":"10.1016/j.jfca.2025.108350","DOIUrl":"10.1016/j.jfca.2025.108350","url":null,"abstract":"<div><div>The electronic nose (E-nose) is a bionic sensing technology that simulates the biological olfactory system and is currently applied in various fields. To identify chili pepper varieties and origins conveniently and accurately, in this study, we developed a novel manifold discriminant extreme learning machine (MDELM) classification model combined with an E-nose to analyze the aroma of chili peppers. First, we collected flavor information from different chili pepper varieties and chili peppers of the same variety from different origins via an E-nose. Second, an MDELM classification model is designed by integrating manifold learning, linear discriminant analysis and maximum variance theory into a unified extreme learning machine framework. Third, we conducted extensive comparative experiments on three chili pepper odor datasets. The experimental results showed that MDELM achieved classification accuracies of 90.40 %, 87.60 %, and 98.80 % on the three datasets, outperforming the other six comparison models, which exhibited excellent performance in identifying chili pepper varieties and origins. Finally, ablation experiments and early recognition experiments were conducted, which indicated that each module of the model improved the model classification performance and that the MDELM can effectively complete early identification tasks for chili pepper odors via an E-nose.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108350"},"PeriodicalIF":4.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Environmentally friendly SLPME of propineb and mancozeb from tomato and rice","authors":"Fernanda C.O.L. Martins , Wanessa R. Melchert","doi":"10.1016/j.jfca.2025.108358","DOIUrl":"10.1016/j.jfca.2025.108358","url":null,"abstract":"<div><div>In this study, a new, eco-friendly, and easy-to-use solid-liquid phase microextraction (SPLME) method was developed for propineb (PB) and mancozeb (MB), enabling the differentiation between them and their determination using Flow Injection Analysis coupled with flame atomic absorption spectroscopy (FIA-FAAS). The extraction and determination of these dithiocarbamates were optimized from food samples, with 400 µL of 1.00 × 10<sup>−2</sup> mol L<sup>−1</sup> EDTA solution in 0.04 mol L<sup>−1</sup> BR buffer at pH 9.50 as the extractor solvent, which is environmentally friendly. This proposed methodology enabled the determination of the MB and PB with detection limits below the residue maximum limit established by regulatory agencies, selectivity between them, and precision and accuracy. Additionally, PB and MB were extracted from tomato and rice samples employing the optimized SPLME conditions, which yielded good recoveries. The obtained concentrations were compared with those obtained through microextraction of total dithiocarbamates, as determined by the colorimetric method for dithiocarbamates, and analyzed using Student's t-test at a 95 % confidence level, revealing no significant difference. Moreover, the greenness metrics (e.g., BAGI, AGREEprep, and ComplexMoGAPI) were utilized in the evaluation of SPLME coupled with FIA-FAAS. Therefore, this method is an eco-friendly and easy operation for extracting PB and MB.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108358"},"PeriodicalIF":4.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145220064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cerium-cysteine nanozymes with dual enzyme-like activities for paper-based intelligent detection of ginkgo acid","authors":"Xueer Lin , Guancheng Zhu , Jiahui Zou, Yuqi Chen, Linfeng Pang, Jiaying Chen, Yu Zhang, Yanyan Huang","doi":"10.1016/j.jfca.2025.108351","DOIUrl":"10.1016/j.jfca.2025.108351","url":null,"abstract":"<div><div>Ginkgo acid (GA), the primary toxic component in ginkgo products, presents significant safety concerns. To overcome the limitations of traditional chromatographic detection methods, this study aimed to construct a cerium-cysteine (noted as “CeCys”) nanozyme with oxidase (OXD)- and peroxidase (POD)-like activities for colorimetric detection of GA. Through the self-assembly of Ce<sup>3 +</sup> and cysteine (Cys), CeCys was constructed. Since GA could inhibit the dual enzyme-like activities of CeCys nanozyme, the developed system could detect GA via colorimetric analysis with the limit of detection (LOD) as 0.71 μg mL<sup>−1</sup> (OXD-like activity) and 0.83 μg mL<sup>−1</sup> (POD-like activity), respectively. The system also exhibited excellent selectivity and anti-interference capabilities. Fourier transform infrared spectrum analysis revealed the binding of GA with CeCys disrupted the coordination between Ce and Cys, thereby inhibiting the active sites. By integrating the OXD-like activity and a test-paper platform with smartphone, the CeCys nanozyme-based detection system could achieve the intelligent detection of GA with a LOD of 2.78 μg mL<sup>−1</sup> and an ultrafast detection time of 2 min. Practical application in ginkgo leaf samples conformed the high accuracy with recovery rates of 94.5 %-114 %. This study might provide significant technical references and theoretical foundations for nanozyme-based rapid platforms for GA detection.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108351"},"PeriodicalIF":4.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carlos E. Lozano-Olvera , Irma Pérez-Silva , Giaan A. Álvarez-Romero , José A. Rodriguez , Jesús Andrés Tavizón-Pozos , Ma. Elena Páez-Hernández
{"title":"Determination of lead and cadmium in oregano, laurel, thyme, green tea and tobacco by flame atomic adsorption spectroscopy using ZnO nanoflowers","authors":"Carlos E. Lozano-Olvera , Irma Pérez-Silva , Giaan A. Álvarez-Romero , José A. Rodriguez , Jesús Andrés Tavizón-Pozos , Ma. Elena Páez-Hernández","doi":"10.1016/j.jfca.2025.108352","DOIUrl":"10.1016/j.jfca.2025.108352","url":null,"abstract":"<div><div>Cd and Pb are toxic heavy metals present in various samples, including oregano, laurel, thyme, green tea and tobacco. Exposure to these metals poses significant health risks, making regular monitoring essential. In this study, an efficient method was developed for the quantification of Pb and Cd in these herbs and in cigarette tobacco by flame atomic absorption spectroscopy (FAAS) employing ZnO nanoflowers as preconcentration material. The ZnO nanoflowers were synthesized via a solvothermal method and characterized by SEM, EDS, XRD and BET techniques, exhibiting a nanoplate morphology with a surface area of 9.8 m<sup>2</sup> g<sup>−1</sup>. Extraction process was optimized using a Taguchi L<sub>9</sub>(3<sup>3</sup>) design, achieving extraction efficiencies of 99.20 % for Pb and 59.35 % for Cd. The preconcentration process yielded enrichment factors of 9.7 and 5.8 for Pb and Cd, respectively. The proposed methodology was validated, demonstrating high accuracy and precision. Metal concentrations in the aforementioned herbs ranged from 0.37 to 18.87 µg g<sup>−1</sup> for Pb and from 0.48 to 2.79 µg g<sup>−1</sup> for Cd, and for tobacco from 0.73 to 18.87 µg g<sup>−1</sup> for Pb and 0.69–1.41 for Cd. This method provides a reliable and cost-effective alternative for monitoring Pb and Cd in herbs and their derived products.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108352"},"PeriodicalIF":4.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Extraction and quantification of aflatoxins and ochratoxin A in dried tamarind from Côte d’Ivoire by LC-MS/MS","authors":"Christine Devouge-Boyer , Balla Ballo , Leonie Moyon , Juliette Vievard , Mathilde Lauzent , Tiemélé Laurent-Simon Amoikon , Solange Aka-Gbezo , Mélanie Mignot","doi":"10.1016/j.jfca.2025.108348","DOIUrl":"10.1016/j.jfca.2025.108348","url":null,"abstract":"<div><div>Tamarind, a widely consumed fruit in Côte d′Ivoire, is often sold in open markets where storage conditions may promote fungal growth. Despite its popularity, little attention has been given to the health risks of mycotoxin contamination in this product. To our knowledge, this is the first study investigating simultaneous aflatoxin and ochratoxin A contamination in tamarind sold in Côte d’Ivoire. This study aimed to develop a simple and reliable method to extract and measure mycotoxins in tamarind. Forty samples were collected from the wholesale markets in three municipalities of Abidjan in Côte d’Ivoire. Using LC-MS/MS analytical method, levels of aflatoxins B1, B2, G1, G2, and ochratoxin A were quantified, following European Commission limits to mitigate health risks associated with this food contamination. The results indicate that aflatoxin levels in all analyzed tamarind samples remained below the acceptable threshold (10 µg/kg for aflatoxins), posing no significant health risk. However, 16 samples exceeded the permitted level for ochratoxin A (above 2 µg/kg). Although improvements in the extraction method and post-clean-up procedures are required to reduce the matrix effect for ochratoxin A, these findings highlight the need for continued monitoring of ochratoxin A contamination in tamarind to ensure consumer safety.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108348"},"PeriodicalIF":4.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nur Hidayah Jamaludin , Ahmad Faizal Abdul Razis , Nuzul Noorahya Jambari , Lay Ching Chai , Lijun You , Muhamad Shirwan Abdullah Sani , Naziruddin Mat Ariffin , Khuzma Din , Maimunah Sanny
{"title":"Multivariate assessment of acrylamide formation and processing variables in fast-food french fries under real-world frying conditions","authors":"Nur Hidayah Jamaludin , Ahmad Faizal Abdul Razis , Nuzul Noorahya Jambari , Lay Ching Chai , Lijun You , Muhamad Shirwan Abdullah Sani , Naziruddin Mat Ariffin , Khuzma Din , Maimunah Sanny","doi":"10.1016/j.jfca.2025.108347","DOIUrl":"10.1016/j.jfca.2025.108347","url":null,"abstract":"<div><div>Acrylamide, a probable human carcinogen formed via the Maillard reaction, remains a key food safety concern in carbohydrate-rich fried foods such as French fries. This study investigates acrylamide formation under real-world frying conditions, integrating precursor composition and operational practices through a multivariate approach. A total of 48 samples (frozen par-fried potato strips and freshly fried French fries) were collected from four major fast-food chains in Malaysia. Acrylamide concentrations were quantified in both frozen par-fried strips and freshly fried fries using LC-MS/MS. In addition, precursor sugars (glucose, fructose, sucrose) and moisture content were analysed only in the frozen par-fried strips using HPLC. On-site frying conditions, including frying time, temperature, and oil usage, were recorded to capture operational variability. Significant differences in acrylamide concentrations were observed among chains (159–609 µg/kg; <em>p</em> < 0.05), and these variations, despite adherence to SOPs, were largely attributable to operational inconsistencies and human factors. Multivariate analyses (XLSTAT) revealed sucrose, frying time, moisture, and fructose as dominant contributors to acrylamide variability, collectively explaining 69.5 % of the total variance. Principal Component Analysis (PCA) demonstrated strong positive associations between acrylamide, sucrose, and fructose, while glucose exhibited an inverse trend. Agglomerative Hierarchical Clustering (AHC) further differentiated chains based on frying and compositional profiles. This study provides the first integrated assessment of precursor composition and operational practices in Malaysian fast-food settings using multivariate modelling. The findings support targeted mitigation strategies through both raw material management and process optimisation to reduce acrylamide risks in commercial French fries.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108347"},"PeriodicalIF":4.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145117814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashish K. Singh, Pawankumar Rai, Deepali Tripathi, Sandeep K. Sharma
{"title":"One-step label-free electrochemical immunoassay for Fumonisin B1 in food using carbodiimide-activated thiolated surfaces","authors":"Ashish K. Singh, Pawankumar Rai, Deepali Tripathi, Sandeep K. Sharma","doi":"10.1016/j.jfca.2025.108345","DOIUrl":"10.1016/j.jfca.2025.108345","url":null,"abstract":"<div><div>Mycotoxins are produced by various fungal species that lead to significant health hazard to both humans and livestock. Fumonisin B1 (FumB1), a mycotoxin primarily produced by Fusarium species contaminates a wide range of food commodities, and pose serious health risks owing to its carcinogenic and neurotoxic effects. Although conventional detection methods for FumB1 offer high sensitivity and accuracy, they often require complex sample preparation and advanced instrumentation, making them unsuitable for on-site testing. Therefore, the development of rapid, sensitive and field-deployable detection method for FumB1 is crucial to mitigate risks associated with FumB1. Here, a one-step label-free electrochemical immunosensor has been developed using a cysteamine (CysAm)-modified screen-printed gold electrode (AuSPE) for the detection of FumB1. The sensor was developed by immobilizing anti-FumB1 antibodies onto the functionalized electrode surface. The developed FumB1 immunosensor demonstrated linear range of detection from 0.001 to 1.0 μg·mL⁻¹ with a calculated limit of detection (LOD) of 0.12 ng·mL⁻¹ . The developed sensor provides rapid and sensitive detection of FumB1, and has potential for on-site testing by the regulatory bodies and food industries to assure food safety and quality control.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"148 ","pages":"Article 108345"},"PeriodicalIF":4.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}