Journal of Food Composition and Analysis最新文献

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A CNN-BiGRU-selfattention model combined with GAN reconstruction and Reverse Feature Fusion for apple pesticide residues detecting
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-26 DOI: 10.1016/j.jfca.2025.107264
Yanshen Zhao, Yifan Zhao, Xinzan Liu, Huayu Fu, Cong Wang, Zhongzhi Han
{"title":"A CNN-BiGRU-selfattention model combined with GAN reconstruction and Reverse Feature Fusion for apple pesticide residues detecting","authors":"Yanshen Zhao,&nbsp;Yifan Zhao,&nbsp;Xinzan Liu,&nbsp;Huayu Fu,&nbsp;Cong Wang,&nbsp;Zhongzhi Han","doi":"10.1016/j.jfca.2025.107264","DOIUrl":"10.1016/j.jfca.2025.107264","url":null,"abstract":"<div><div>Pesticide residues in fruits, particularly apples, pose a significant threat to human health, highlighting the urgent need for precise and efficient detection methods. Although spectral detection techniques have emerged as a promising lossless solution, their effectiveness is often hindered by challenges such as imbalanced data distribution and redundant spectral features. To address these limitations, we propose a novel method that combines spectral reconstruction and key wavelength selection. This method introduces a CNN-BiGRU-Selfattention (CBS) model, which utilizes Generative Adversarial Networks (GAN) for spectral reconstruction and Reverse Feature Fusion (RFF) for selecting key wavelengths. By tackling these critical issues, the proposed approach achieves significant improvements in pesticide residue detection accuracy for apples. Experimental results on apple datasets demonstrated exceptional performance, with an accuracy of 96.31 %, recall of 96.31 %, precision of 0.9632, and an F1 score of 0.9630. These results underscore the model's robustness and effectiveness in extracting critical spectral features, ensuring reliable detection. By enhancing the accuracy and reliability of pesticide residue detection, this study offers a pioneering approach to safeguard food safety and lays a foundation for advancing future research in agricultural applications.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107264"},"PeriodicalIF":4.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of folate (vitamin B9) composition and accumulation pattern in developing kernels of specialty and biofortified maize genotypes
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-26 DOI: 10.1016/j.jfca.2025.107259
Kothalanka Victor Paul, Rajkumar Uttamrao Zunjare , Firoz Hossain, Vignesh Muthusamy, Subhra Jyotshna Mishra, Ashvinkumar Katral, Prafulla Kumar
{"title":"Analysis of folate (vitamin B9) composition and accumulation pattern in developing kernels of specialty and biofortified maize genotypes","authors":"Kothalanka Victor Paul,&nbsp;Rajkumar Uttamrao Zunjare ,&nbsp;Firoz Hossain,&nbsp;Vignesh Muthusamy,&nbsp;Subhra Jyotshna Mishra,&nbsp;Ashvinkumar Katral,&nbsp;Prafulla Kumar","doi":"10.1016/j.jfca.2025.107259","DOIUrl":"10.1016/j.jfca.2025.107259","url":null,"abstract":"<div><div>The present study evaluated 48 maize inbreds including biofortified and specialty-corn inbreds for folate and its major derivatives, at three stages of kernel development-15, 30, and 45 days after pollination (DAP). Significant effects of genotype, DAP stage, and genotype × DAP stage interactions were observed for all traits. Significant variability for 5-methyltetrahydrofolate (5-MTHF), 5-formyltetrahydrofolate (5-FTHF), and total folate content across the different stages highlighting genetic control for folate accumulation. The mean total folate content was 75.0 µg/100 g with range of 22.7-133.1 µg/100 g across the kernel developmental stages. The study also revealed a metabolic shift from 5-MTHF to 5-FTHF during kernel development. Folate levels of genotypes exhibited a declining trend as the kernel matured. Sweet- and waxy- corn had the highest folate levels, than other corn types. The high folate lines identified such as PMI-SWT016, PMI-PV7, MGU213wx, PMI-SWT020, and MGU203W can be used as donors for future biofortification programmes.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"141 ","pages":"Article 107259"},"PeriodicalIF":4.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-nutritional factors and heavy metals in edible seaweeds: Challenges, health implications, and strategies for safer consumption
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107283
Neelesh Prashant, Monika Sangwan, Priyanka Singh, Pallabi Das, Utkal Srivastava, Felix Bast
{"title":"Anti-nutritional factors and heavy metals in edible seaweeds: Challenges, health implications, and strategies for safer consumption","authors":"Neelesh Prashant,&nbsp;Monika Sangwan,&nbsp;Priyanka Singh,&nbsp;Pallabi Das,&nbsp;Utkal Srivastava,&nbsp;Felix Bast","doi":"10.1016/j.jfca.2025.107283","DOIUrl":"10.1016/j.jfca.2025.107283","url":null,"abstract":"<div><div>Seaweeds are a vital part of the human diet in many East-Asian and Pacific and have gained popularity in some European and South American countries, too. This can be attributed to their unique texture, taste, diverse nutritional profiles and nutraceutical properties. However, despite being a nutritious food, they contain anti-nutrients form insoluble complexes, bind essential minerals, and reduce their digestibility and availability Furthermore, the accumulation of heavy metals in seaweed poses health risks such as neurological, developmental, and organ damage. These components raise concerns about their overall nutritional value and safety. This review article discusses the presence of anti-nutrients and heavy metals in seaweed and their potential effects on human health. We also focus on possible pre-harvest practices and post-harvest processing techniques that can be used to reduce anti-nutrients and heavy metals for safer seaweed consumption. The findings underscore that with optimized cultivation and processing, seaweeds can be transformed into a safe, nutritious, and environmentally sustainable food source, contributing meaningfully to global food security and public health.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107283"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mass spectrometry differentiation of sciadonic acid isomers and estimate of long-chain polyunsaturated fatty acid intake from fresh and processed duck eggs
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107253
Chuanshun Dai , Lerong Qi , Tingxiang Yang , Zhen Wang , Dong Hao Wang
{"title":"Mass spectrometry differentiation of sciadonic acid isomers and estimate of long-chain polyunsaturated fatty acid intake from fresh and processed duck eggs","authors":"Chuanshun Dai ,&nbsp;Lerong Qi ,&nbsp;Tingxiang Yang ,&nbsp;Zhen Wang ,&nbsp;Dong Hao Wang","doi":"10.1016/j.jfca.2025.107253","DOIUrl":"10.1016/j.jfca.2025.107253","url":null,"abstract":"<div><div>Sciadonic acid, or 20:3(5Z,11Z,14Z), is an unusual polyunsaturated fatty acid (PUFA) found in certain gymnosperm nuts and marine invertebrates. However, no study has detected it in commonly consumed food items. This study analyzed the fatty acid compositions of fresh, salted, and preserved duck eggs thoroughly via gas chromatography coupled with covalent adduct chemical ionization mass spectrometry (GC/CACI-MS). A multiple reaction monitoring method was developed to specifically characterize three 20:3 isomers found in duck eggs. Sciadonic acid was quantified at 0.13 % and 0.07 % (wt/wt of total fatty acids) in fresh and processed duck eggs, respectively. The major n-3 PUFA is DHA, with average concentrations of 1.44 %, 0.79 %, and 0.98 % in fresh, preserved, and salted duck eggs, respectively. For an average Chinese individual, the daily intake of DHA and total long-chain n-3 PUFA from all types of duck eggs is estimated to be 7.6 mg/day and 10.6 mg/day, respectively.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107253"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microplastics in food: Sources, distribution, health impacts, and regulation
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107274
Jinzhao Xu , Mengqi Tang , Xiaoxi Xu
{"title":"Microplastics in food: Sources, distribution, health impacts, and regulation","authors":"Jinzhao Xu ,&nbsp;Mengqi Tang ,&nbsp;Xiaoxi Xu","doi":"10.1016/j.jfca.2025.107274","DOIUrl":"10.1016/j.jfca.2025.107274","url":null,"abstract":"<div><div>Microplastics (MPs) refer to microparticles and nanoparticles continuously released during the degradation of plastic waste and the use of plastic products. MP pollution in the environment is particularly persistent, particularly due to the stable and refractory nature of plastic waste. Compared with large plastic particles, MPs are more likely to spread through air, water, and other media, ultimately entering the human body through the food chain. Subsequently, MPs accumulate in a wide range of distributions throughout the body, including the gut, brain, immune system, and respiratory system, leading to chronic inflammation and disease, and even acting as vectors for other pathogens and contaminants that further compromise overall health. However, our understanding of the sources, distribution, detection methods, and human health impacts of MPs in food is limited. In this review, we provide an overview of the sources, environmental distribution, and potential health impacts of MPs in food (marine food, terrestrial food, water sources, and food materials and processing). We also examine strategies for controlling and reducing the accumulation of MPs in food. Finally, we highlight the challenges and prospects for future research. This review provides scientific evidence to support further studies on MPs in food.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107274"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143352347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Determination of aflatoxin M1 levels in cheese in Iran’s market
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107267
Amirhossein Abedini , Parisa Sadighara , Behrouz Akbari-Adergani , Soheyl Eskandari , Mahmood Yousefi
{"title":"Determination of aflatoxin M1 levels in cheese in Iran’s market","authors":"Amirhossein Abedini ,&nbsp;Parisa Sadighara ,&nbsp;Behrouz Akbari-Adergani ,&nbsp;Soheyl Eskandari ,&nbsp;Mahmood Yousefi","doi":"10.1016/j.jfca.2025.107267","DOIUrl":"10.1016/j.jfca.2025.107267","url":null,"abstract":"<div><div>Dairy products, particularly cheese, are prone to contamination by aflatoxin M1 (AFM1), a toxic compound generated by Aspergillus fungi. This study aims to determine the content of AFM1 in various types of cheese using high-performance liquid chromatography with fluorescence detection. Among the 109 cheese samples analyzed (probiotic, ripened in brine, pizza, UF, cream cheese, pasteurized white cheese, gouda, processed cheese, traditional, and mozzarella), 61.3 % were found to contain detectable levels of AFM1. There was a significant difference (<em>p</em> = 0.036) between cheese ripened in brine (0.05674 µg/kg) and traditional cheese (0.00169 µg/kg). A slight difference (<em>p</em> = 0.057) was observed between traditional cheese (0.00169 µg/kg) and white pasteurized cheese (0.05217 µg/kg). These findings emphasize the importance of ongoing monitoring and regulatory measures to mitigate AFM1 contamination in dairy products and ensure consumer safety.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107267"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trace copper determination in mate tea and tap water using FAAS and spray-assisted liquid phase microextraction
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107281
Kübra Karakebap , Hakan Serbest , Fatma Turak , Sezgin Bakırdere
{"title":"Trace copper determination in mate tea and tap water using FAAS and spray-assisted liquid phase microextraction","authors":"Kübra Karakebap ,&nbsp;Hakan Serbest ,&nbsp;Fatma Turak ,&nbsp;Sezgin Bakırdere","doi":"10.1016/j.jfca.2025.107281","DOIUrl":"10.1016/j.jfca.2025.107281","url":null,"abstract":"<div><div>In the present study, a rapid, efficient and environmentally friendly analytical approach was proposed for the determination of trace copper by flame atomic absorption spectrometry (FAAS) after spray-assisted fine droplet formation based liquid phase microextraction (SFDF-LPME), which eliminates the use of dispersing solvents. With the help of a simple nozzle apparatus, an efficient distribution of the extraction solvent into the sample was achieved. The developed SFDF-LPME-FAAS method achieved a limit of detection (LOD) of 1.6 µg L<sup>−1</sup> and a limit of quantification (LOQ) of 5.2 µg L<sup>−1</sup> under optimal conditions. A wide linear range was recorded with a regression coefficient (R<sup>2</sup>) of 0.9987 between 5.0 and 100 µg L<sup>−1</sup>. The improvement in sensitivity was found to be 57.4-fold by comparing the slopes of the calibration plot equation of FAAS and SFDF-LPME-FAAS systems. The feasibility of the SFDF-LPME-FAAS method was evaluated by recovery studies using mate tea extracts and tap water samples. The good recovery results obtained for tap water samples and mate tea extracts in the range of 81.1 % - 113.8 % and 85.5 % - 120.8 %, respectively, showed that the method is applicable to similar matrices.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107281"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of pear fruit quality in different ripening stages based on internal quality characteristics
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107282
Chen Yin, Luming Tian, Jing Li, Yufen Cao, Xingguang Dong, Hongliang Huo, Jiayu Xu, Chao Liu
{"title":"Evaluation of pear fruit quality in different ripening stages based on internal quality characteristics","authors":"Chen Yin,&nbsp;Luming Tian,&nbsp;Jing Li,&nbsp;Yufen Cao,&nbsp;Xingguang Dong,&nbsp;Hongliang Huo,&nbsp;Jiayu Xu,&nbsp;Chao Liu","doi":"10.1016/j.jfca.2025.107282","DOIUrl":"10.1016/j.jfca.2025.107282","url":null,"abstract":"<div><div>The impact of maturity on pear fruit quality directly affects consumer taste. Ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used to determine the sugar and organic acid components of mature pear fruits with different texture types, a texture analyzer was used to determine the texture traits of pear fruits. The result revealed that the variation tendency of sugar and organic acid components affected by the texture types, ripening stages and shelf-life. The decrease in flesh firmness of pear fruits was related to sucrose and glucose during the shelf-life of 0–14 days (d) at different ripening stages. According to the principal component analysis, Nanguoli, Jingbaili and Chili achieved the highest fruit quality score when harvested at 136 days after flower bloom (DAFB); Gute Luise and Korla pear achieved the highest fruit quality score when harvested at 146 DAFB; Yali and Dangshan Suli achieved the highest fruit quality score when harvested at 156 DAFB. In conclusion, combination of texture, sugar and organic acid components could serve as powerful maturity indicator candidates, provided valuable references for determining appropriate harvesting period and the best shelf-life quality of pear fruits.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107282"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preventing alkali-injury in heavy metal-free preserved eggs: Model development
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107285
Baochang Li , Yishu Wang , Ruipeng Ma , Jialei Wang , Qifei Wu , Jun Sun
{"title":"Preventing alkali-injury in heavy metal-free preserved eggs: Model development","authors":"Baochang Li ,&nbsp;Yishu Wang ,&nbsp;Ruipeng Ma ,&nbsp;Jialei Wang ,&nbsp;Qifei Wu ,&nbsp;Jun Sun","doi":"10.1016/j.jfca.2025.107285","DOIUrl":"10.1016/j.jfca.2025.107285","url":null,"abstract":"<div><div>The development of heavy metal-free preserved eggs (HMF-PE) has become a focal point of interest. Given the absence of plugging effect of heavy metals, the key in HMF-PE process is to quickly remove HMF-PE from the curing tank when the gel quality of its egg white (PEW) reaches peak to prevent \"alkali injury\". Our research confirms that the free alkalinity in the PEW conforms to a first-order kinetic model at 15, 20, and 25 ℃. Ultimately, based on the Gaussian equation, a predictive model for the comprehensive quality score of the PEW based on free alkalinity was constructed. Using this model to determine the optimal tank exit timing has resulted in a quality of HMF-PE that rivals commercial products, especially for those cured for 11 days at 25 ℃. This study establishes a theoretical foundation for the strategic harvesting of HMF-PE at the peak of their gel quality.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107285"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Voltammetric genosensor from silica nanocomposites for transgenic soybean analysis
IF 4 2区 农林科学
Journal of Food Composition and Analysis Pub Date : 2025-01-25 DOI: 10.1016/j.jfca.2025.107277
Ling Ling Tan , Dedi Futra , Lee Yook Heng , Alizar Ulianas , Adlin Azlina Abdul Kadir , Zamri Ishak
{"title":"Voltammetric genosensor from silica nanocomposites for transgenic soybean analysis","authors":"Ling Ling Tan ,&nbsp;Dedi Futra ,&nbsp;Lee Yook Heng ,&nbsp;Alizar Ulianas ,&nbsp;Adlin Azlina Abdul Kadir ,&nbsp;Zamri Ishak","doi":"10.1016/j.jfca.2025.107277","DOIUrl":"10.1016/j.jfca.2025.107277","url":null,"abstract":"<div><div>The introduction of genetically modified organisms (GMOs) to crops has rendered continuous concern and debates on GM foods, which led to the requirement of appropriate labelling of GMO-derived products and the demands of advanced technologies for on-site detection of transgenic crops. In this study, an electrochemical genosensor for rapid, ultrasensitive, and specific determination of cauliflower mosaic virus (CaMV35S) gene in GM soybean based on silica-gold nanoparticles composite electrode was developed. Colloidal gold nanoparticles were drop-coated onto a carbon screen-printed electrode (SPE) followed by amine-functionalized silica nanospheres to obtain a SiO<sub>2</sub>-Au nanocomposite electrode (SiO<sub>2</sub>-Au-SPE). Aminted DNA probes were grafted onto the SiO<sub>2</sub>-Au-SPE using glutaric dialdehyde crosslinking agent. Meanwhile, thiolated DNA probes were directly chemisorbed onto the AuNPs-modified SPE for comparison purposes. Under optimum conditions, the DNA biosensor based on SiO<sub>2</sub>-Au-SPE could determine the target CaMV35S gene in a linear DNA concentration range of 1.0 × 10<sup>−14</sup> M to 1.0 × 10<sup>−8</sup> M, with a limit of detection (LOD) of 2.37 fM cDNA. Glutaraldehyde crosslinking of aminated DNA probe to the aminated SiO<sub>2</sub> nanospheres attained stronger bonding strength with a 10-fold higher calibration sensitivity towards cDNA compared to direct chemisorption of thiolated DNA probe to the AuNPs by thiol chemistry. The validation study showed that the electrochemical DNA biosensor based on SiO<sub>2</sub>-Au nanocomposite electrode was comparable to the conventional polymerase chain reaction (PCR) method for the evaluation of transgenic soybean contents in soy food samples, with 99.1–101.3 % recoveries of GM DNA.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"140 ","pages":"Article 107277"},"PeriodicalIF":4.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143216841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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