{"title":"Electrochemical determination of Hg(II) in aqueous samples using electromembrane extraction combined with vortex-assisted switchable solvent","authors":"Siamak Kiani Shahvandi , Mehrorang Ghaedi , Hamid Ahmar , Pouya Karimi , Hamedreza Javadian","doi":"10.1016/j.jfca.2024.106876","DOIUrl":"10.1016/j.jfca.2024.106876","url":null,"abstract":"<div><div>This research paper presents a novel methodology that integrates electrochemical techniques, specifically electromembrane extraction (EME) and vortex-assisted switchable hydrophilicity solvent-based liquid-phase microextraction (VASHS-LPME). Electrochemical analysis allows for the sensitive detection of Hg(II). The study investigated the impact of vortexing during the switch of the extraction solvent for the first time. Additionally, it explored the potential of vortexing as an alternative to time-consuming temperature control processes, the use of hazardous acids like HCl, and dry ice. The study examined the utilization of potato dextrose agar (PDA) gel as a membrane in EME, eliminating the need for an organic solvent in Hg(II) extraction. We optimized the experimental conditions and found that by meeting specific parameters, we could efficiently extract Hg(II) from a 20 mL solution sample using a PDA gel-based membrane in an aqueous acceptor phase. Subsequently, the VASHS-LPME method was applied under optimal conditions for the extraction of Hg(II) ions. The study demonstrated a linear range of 0.0075–100 μmol L<sup>−1</sup>, with a limit of quantification (LOQ) and limit of detection (LOD) ranging from 0.0075 to 0.0022 μmol L<sup>−1</sup>. The precision values obtained for intra- and inter-day relative standard deviations (RSDs) were 3.8 % and 4.3 %, respectively. The technique proposed in the research was effectively employed to determine the quantity of Hg(II) in food and water samples.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106876"},"PeriodicalIF":4.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dejin Wang , Xingqiang Wu , Jingchun Mao , Zongyi Wang , Yujie Xie , Xudong Wu , Kaixuan Tong , Hongping Man , Chunlin Fan , Hui Chen
{"title":"A modified QuEChERS method for the generic and rapid determination of pesticides and mycotoxins in raw coffee beans by liquid chromatography-quadrupole-time-of-flight mass spectrometry","authors":"Dejin Wang , Xingqiang Wu , Jingchun Mao , Zongyi Wang , Yujie Xie , Xudong Wu , Kaixuan Tong , Hongping Man , Chunlin Fan , Hui Chen","doi":"10.1016/j.jfca.2024.106928","DOIUrl":"10.1016/j.jfca.2024.106928","url":null,"abstract":"<div><div>In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges (<em>R</em><sup><em>2</em></sup>> 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106928"},"PeriodicalIF":4.0,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of colorimetric multiplex loop mediated isothermal amplification combined with lateral flow dipstick (mLAMP-LFD) assay for the identification of TDH toxin-producing Vibrio parahaemolyticus","authors":"Soottawat Benjakul , Phutthipong Sukkapat , Suriya Palamae , Prashant Singh , Mingkwan Yingkajorn , Jirayu Buatong , Jirakrit Saetang","doi":"10.1016/j.jfca.2024.106924","DOIUrl":"10.1016/j.jfca.2024.106924","url":null,"abstract":"<div><div><em>Vibrio parahaemolyticus</em> in seafood is associated with gastroenteritis diseases worldwide. Rapid and sensitive detection methods of the pathogenic strain are essential for monitoring and preventing the diseases. Colorimetric multiplex loop-mediated isothermal amplification combined with lateral flow dipstick assay (mLAMP-LFD) was developed to differentiate pathogenic strains from non-pathogenic <em>V. parahaeholyticus</em>. Thermolabile hemolysin (<em>TLH</em>) and thermo-stable direct hemolysin (<em>TDH</em>) genes were designed and used for mLAMP-LFD assay. The assay specificity was validated using strains belonging to 11 species of other foodborne pathogens, while the sensitivity was evaluated by using a DNA template ranging from 0.005 to 0.4 ng/reaction. Shrimp samples inoculated at 10<sup>1</sup>, 10<sup>2</sup>, and 10<sup>3</sup> CFU/ 10 g, and enrichment times of 0, 2, 4, 6, and 8 h were used for assay validation. The standardized assay accurately identified 20 <em>V. parahaemolyticus</em> strains and showed no cross-amplification. The assay’s limit of detection was 0.02 ng and took 30 min to complete. Shrimp samples inoculated at 10 CFU of <em>V. parahaemolyticus</em> could be detected following a four-hour enrichment. In conclusion, the high sensitivity and specificity of the mLAMP-LFD assay developed in the present study could be used to identify pathogenic strains of <em>V. parahaemolyticus</em>.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106924"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Can fragment ion intensity be used for fatty acid sn-position determination of food phospholipids?","authors":"Wenjiao Wang , Zhiqian Liu , Simone Rochfort","doi":"10.1016/j.jfca.2024.106923","DOIUrl":"10.1016/j.jfca.2024.106923","url":null,"abstract":"<div><div>Fatty acid (FA) regio-position is critical information for the structures of both triglycerides and phospholipids in food products. Although lipase/phospholipase hydrolysis combined with TLC separation and GC analysis can be used to determine the overall sn-2 FA composition of triglycerides/phospholipids in food samples, it is much more challenging to pinpoint FA regio-position at the molecular species level. Currently, the most widely used method to obtain such information is based on the relative intensity of fragment ions generated in tandem mass spectrometry. The reliability of this approach was verified in this study using regio-pure phospholipid standards and by cross-validation with the Paternò–Büchi photochemical reaction. We have demonstrated that for food materials with widespread regioisomers, a standard calibration curve from regio-pure standards is needed to determine the proportion of each isomer, whereas fragment ion intensity can only be used to reveal the sn-position of FA in phospholipid molecules devoid of regioisomers.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106923"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Maria Kochetkova, Irina Timofeeva, Daria Frolova, Andrey Bulatov
{"title":"Low-cost digital colorimetric sensor for rapid on-site determination of ascorbic acid in vegetable- and fruit-based purees and juices","authors":"Maria Kochetkova, Irina Timofeeva, Daria Frolova, Andrey Bulatov","doi":"10.1016/j.jfca.2024.106920","DOIUrl":"10.1016/j.jfca.2024.106920","url":null,"abstract":"<div><div>Ascorbic acid is essential for human health due to its potent antioxidant properties; however, excessive intake can lead to adverse effects. This has created a high demand for simple and cost-effective analytical techniques to determine this analyte outside of laboratory settings for food quality control. In this study, a portable colorimetric sensor was developed for the quantitative <em>on-site</em> determination of ascorbic acid. The technique is based on the reduction of phosphorus heteropoly acid by ascorbic acid, resulting in the formation of a blue-colored product. The heteropoly acid was immobilized on a polymer template in the sensor's indicator zone with menthol used as an environmentally friendly extractant to enhance the device’s stability and extend its shelf life. The colorimetric sensor demonstrated reliable detection with a low limit of detection of 5 mg kg<sup>−1</sup>, calculated based on a blank test using 3σ. Under optimized conditions, the linear detection range was 15–150 mg kg<sup>−1</sup>. The disposable sensors were applied to determine ascorbic acid in fruit and vegetable juices and purees for baby food, with recovery rates between 88 % and 110 %. This easy-to-produce and ready-to-use technique presents significant potential for mass <em>on-site</em> screening of ascorbic acid in food samples.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106920"},"PeriodicalIF":4.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Measuring and modelling Syrah red wines evolution: Ageing tests, color and new molecular indices","authors":"Luca Garcia, François Garcia, Cédric Saucier","doi":"10.1016/j.jfca.2024.106912","DOIUrl":"10.1016/j.jfca.2024.106912","url":null,"abstract":"<div><div>A Syrah red wine ageing experiment was set up during 24 months to measure the influence of different micro-agglomerated corks on wine composition and color. Two new wine ageing indices based on targeting phenolic composition (I<sub>ox</sub>, oxidation index and I<sub>su</sub>, sulfonation index) or colorimetric one (Hue) are proposed. The results showed that the slopes of evolution of these indices varied according to the wine as well as the index studied, and that the initial composition played a predominant role in these evolutions. In addition, the oxygen transfer rate (OTR) of the cork modulates the rate of evolution of the oxidation index and color, but had no impact on the sulfonation index. Partial least squares (PLS) regressions were used in order to establish some models that can predict the ageing index slope based on accelerated ageing tests (AATs), voltammetric parameters and phenolic composition measured before bottling. The models involved different explaining variables for each test and showed good predictive capacities with correlation coefficients (R²) > 0.79.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106912"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Automated simultaneous determination of mercury and copper in edible oils by reversed-phase column-based extraction with deep eutectic solvent","authors":"Andrey Shishov , Sergey Savinov , Nipu Kumar Das , Tamal Banerjee , Andrey Bulatov","doi":"10.1016/j.jfca.2024.106919","DOIUrl":"10.1016/j.jfca.2024.106919","url":null,"abstract":"<div><div>In this study, a new automated method for the simultaneous determination of mercury and copper in edible oils using deep eutectic solvents and inductively coupled plasma optical emission spectrometry was developed and tested. The developed method uses a deep eutectic solvent consisting of choline chloride, thiourea and lactic acid to effectively extract mercury and copper from edible oil matrices. The extraction process involves automatically passing an oil sample through a microcolumn with the deep eutectic solvent retained as a stationary phase on glass fiber. This approach significantly reduces sample preparation time and improves reproducibility. The established LODs were 0.13 μg L⁻¹ for mercury and 0.12 μg L⁻¹ for copper, while the LOQs were 0.4 μg L⁻¹ and 0.36 μg L⁻¹, respectively. The method demonstrated high accuracy, with relative standard deviations for repeatability and inter-day reproducibility ranging from 2 % to 9 %. The extraction recovery of both metals exceeded 95 %, confirming the efficiency of the DES-based extraction process. An environmental assessment of the method using the AGREEprep tool showed a favorable environmental index of 0.63.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106919"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Federica Litrenta , Carmelo Cavallo , Matteo Perini , Silvia Pianezze , Enrico D’alessandro , Vincenzo Lo Turco , Giuseppa Di Bella , Luigi Liotta
{"title":"Preliminary study on the influence of the geographical origin and farming system on ‘Nero dei Nebrodi’ pig using chemical and isotopic fingerprinting","authors":"Federica Litrenta , Carmelo Cavallo , Matteo Perini , Silvia Pianezze , Enrico D’alessandro , Vincenzo Lo Turco , Giuseppa Di Bella , Luigi Liotta","doi":"10.1016/j.jfca.2024.106918","DOIUrl":"10.1016/j.jfca.2024.106918","url":null,"abstract":"<div><div>In this study, the geographical origin of <em>longissimus dorsi</em> meat from 'Nero dei Nebrodi' pigs reared in two distinct regions of north-eastern Sicily was indagated by correlating chemical-nutritional parameters, stable isotope composition, fatty acid and sterol profiles, and mineral element content. Significant differences (p ≤ 0.05) between the 'Nebrodi group' (NG) and the 'External Nebrodi group' (ENG) were found for 51 over 80 variables. The <em>δ</em><sup>2</sup>H, <em>δ</em><sup>18</sup>O, <em>δ</em><sup>13</sup>C and <em>δ</em><sup>15</sup>N of the defatted meat were statistically different (p<0.01) between NG and ENG animals, indicating a change in the composition of the diet and drinking water consumed. The results showed that the characteristic rich endemic vegetation of the Nebrodi influences not only the chemical-nutritional parameters of the meat but also its isotopic ratios, allowing for a geographical characterization required for the traceability of this peculiar product, aspiring to a protected designation of origin.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106918"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The high share of steryl esters is responsible for the unusual sterol pattern of black goji berries","authors":"Yan Zheng , Katja Lehnert , Walter Vetter","doi":"10.1016/j.jfca.2024.106921","DOIUrl":"10.1016/j.jfca.2024.106921","url":null,"abstract":"<div><div>Phytosterols are bioactive and healthy components of the human diet. Although numerous investigations reported the rich nutrients in black goji berries, comprehensive data on the sterol pattern were missing. In this study, gas chromatography with mass spectrometry (GC/MS) analysis enabled the detection of 23 sterols and 22 fatty acids in dried black goji berry samples. Black goji berries were dominated by ∆5-avenasterol (mean: 23.7 %) > the rare campesta-5,24(25)-dienol (mean: 19.2 %) > <em>β</em>-sitosterol (mean: 14.9 %). Investigations of the reasons for this unique sterol pattern showed that ∼76% of the sterols in black goji berries were esterified with fatty acids (steryl esters). This share of steryl esters was higher than in virtually all other plant samples where free sterols are predominant. Remarkably, the rare campesta-5,24(25)-dienol was most prominent in the steryl ester fraction. Manual separation and analyses of seeds and pulp subsamples showed that most of the sterols were found in seeds where steryl esters were predominant (∼71 %), while the pulp was dominated by free sterols (∼73 %). Hence, the unconventional sterol pattern of black goji berries mainly originated from the high share of steryl esters in the seeds in combination with different sterol patterns in free and esterified sterols.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106921"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
César O. Arévalo-Hernández , María Claudia Rivero Paucar , Enrique Arévalo-Gardini , Fiorella Barraza
{"title":"Health risk assessment of trace elements in traditional and common foodstuffs from markets in San Martin, Peru","authors":"César O. Arévalo-Hernández , María Claudia Rivero Paucar , Enrique Arévalo-Gardini , Fiorella Barraza","doi":"10.1016/j.jfca.2024.106913","DOIUrl":"10.1016/j.jfca.2024.106913","url":null,"abstract":"<div><div>Non-essential trace elements (TEs) in food may cause adverse health effects. In this study, we evaluated the concentration of Ag, As, Ba, Cd, Pb, Sb, Tl, and V in 17 foodstuffs consumed in five cities located in the department of San Martin, in the Peruvian highland Amazon jungle. These included condiments, nuts, fruits, starches, and vegetables. To minimize the risks of cross-contamination and to ensure data quality, digestion and ICP-MS analyses were carried out in a metal-free, ultra-clean laboratory.</div><div>Barium was the most abundant element at concentrations in the mg/kg range, whereas the rest of the elements were present at trace levels (µg/kg). Only 30 % of the samples exceeded the international thresholds for Cd and Pb, with the greatest levels for Pb found in grapes (3.6 mg/kg) and for Cd in tomatoes (1.5 mg/kg).</div><div>According to the Hazard Index, both adults and children may develop non-carcinogenic effects if exposed to these elements. The cancer risk for As consumption was below the US EPA recommend values.</div><div>Our results highlight the importance of conducting more research to set food safety policies, especially in Latin America, aiming to reduce exposure of the population by adherence to regulations and enforcement by local officials.</div></div>","PeriodicalId":15867,"journal":{"name":"Journal of Food Composition and Analysis","volume":"137 ","pages":"Article 106913"},"PeriodicalIF":4.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}