G-quadruplex/hemin DNAzyme mediated biosensor for colorimetric detection of trace aflatoxin B1 using entropy-driven catalytic reaction-facilitated catalytic hairpin assembly

IF 4 2区农林科学 Q2 CHEMISTRY, APPLIED

Journal of Food Composition and Analysis Pub Date : 2025-04-10 DOI:10.1016/j.jfca.2025.107622

Dian Yu , Yingying Sun , Xin Lin , Wenjie Liu , Jianna Yu , Guoxing Jing , Wen Liu , Xiaoyun Zhang , Cheng Jiang , Wenshan Li

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引用次数: 0

Abstract

G-quadruplex/hemin DNAzyme (G-DNAzyme) is widely utilized in biosensors due to its ability to catalyze peroxidase-like reactions. However, its relatively low catalytic efficiency can limit detection sensitivity. To address this limitation and enable trace detection, this study presents a G-DNAzyme-mediated biosensor that combines entropy-driven catalytic reactions (EDC) and catalytic hairpin assembly (CHA) to enhance sensitivity and broaden its applicability for detecting trace levels of aflatoxin B1 (AFB1). In this biosensor, AFB1 specifically binds to aptamer, triggering the release of a complementary initiator chain, which activates both EDC and CHA amplification processes. This dual amplification results in the generation of numerous free G-quadruplex sequences, which then bind to hemin, catalyzing a color change in the chromogenic substrate and significantly amplifying the signal. The biosensor demonstrated excellent linearity between the absorbance intensity and logarithm of AFB1 concentrations, with a detection limit (LOD) of 0.47 pM. Furthermore, spiked recovery tests of AFB1 in actual samples showed recovery rates between 96.12 % and 108.50 %, indicating strong accuracy. The method is simple, rapid, and does not require complex instrumentation, making it an efficient tool for detecting AFB1 in food safety applications.

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利用熵驱动催化反应-催化发夹组装的g -四重体/hemin DNAzyme介导的生物传感器比色检测痕量黄曲霉毒素B1

g -四plex/hemin DNAzyme （G-DNAzyme）因其催化过氧化物酶样反应的能力而广泛应用于生物传感器中。但其较低的催化效率限制了检测灵敏度。为了解决这一限制并实现痕量检测，本研究提出了一种g - dnazyme介导的生物传感器，该传感器结合了熵驱动的催化反应（EDC）和催化发夹组装（CHA），以提高灵敏度并扩大其检测痕量黄曲霉毒素B1 （AFB1）水平的适用性。在这种生物传感器中，AFB1特异性地与适体结合，触发互补引发链的释放，从而激活EDC和CHA扩增过程。这种双重扩增导致大量自由g -四重体序列的产生，这些序列随后与血红蛋白结合，催化显色底物的颜色变化，并显着放大信号。该生物传感器的吸光度强度与AFB1浓度的对数呈良好的线性关系，检测限为0.47 pM。实际样品中AFB1的加标回收率为96.12 % ~ 108.50 %，准确度较高。该方法简单，快速，不需要复杂的仪器，使其成为食品安全应用中检测AFB1的有效工具。

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来源期刊

Journal of Food Composition and Analysis 工程技术-食品科技

CiteScore

6.20

自引率

11.60%

发文量

601

审稿时长

53 days

期刊介绍： The Journal of Food Composition and Analysis publishes manuscripts on scientific aspects of data on the chemical composition of human foods, with particular emphasis on actual data on composition of foods; analytical methods; studies on the manipulation, storage, distribution and use of food composition data; and studies on the statistics, use and distribution of such data and data systems. The Journal''s basis is nutrient composition, with increasing emphasis on bioactive non-nutrient and anti-nutrient components. Papers must provide sufficient description of the food samples, analytical methods, quality control procedures and statistical treatments of the data to permit the end users of the food composition data to evaluate the appropriateness of such data in their projects. The Journal does not publish papers on: microbiological compounds; sensory quality; aromatics/volatiles in food and wine; essential oils; organoleptic characteristics of food; physical properties; or clinical papers and pharmacology-related papers.