Indonesian Journal of Medical Laboratory Science and Technology最新文献_第4页

Assessment of Platelet Indices Profile of Pregnant Women Attending University of Abuja Teaching Hospital, Nigeria 尼日利亚阿布贾大学教学医院孕妇血小板指数分析

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2021-10-30 DOI: 10.33086/ijmlst.v3i2.2110

A. Dangana, A. Emeribe, Hezekiah Alkali Isah, S. Musa, J. Abu, Solomon Oloche Onoja, N. Onukegbe, I. Abdullahi

{"title":"Assessment of Platelet Indices Profile of Pregnant Women Attending University of Abuja Teaching Hospital, Nigeria","authors":"A. Dangana, A. Emeribe, Hezekiah Alkali Isah, S. Musa, J. Abu, Solomon Oloche Onoja, N. Onukegbe, I. Abdullahi","doi":"10.33086/ijmlst.v3i2.2110","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.2110","url":null,"abstract":"Platelets initiate hemostasis by aggregating at the site of injury and participate in ensuring endothelial integrity. A defect in this process could lead to intravascular blood loss. This case-control study sought to determine the platelet counts and indices among pregnant women in the University of Abuja Teaching Hospital Gwagwalada, Abuja, Nigeria. A total of 120 pregnant women as case and 60 non-pregnant women as control were enrolled for this study. Blood samples were collected in EDTA tubes, and complete platelet count and indices were carried out using an automated five-part haematology analyzer. The mean ± standard deviation of the platelets count among the pregnant women, 226.54 ± 69.76 109 cells/L was not significantly different from that of the non-pregnant women, 214.95 ± 52.22x 109 cells/L (p=0.295). There was a significant differences in mean platelets volume (MPV) of the case and control groups (p=0.036). After post-hoc test, the significant difference was between the pregnant women in 3rd trimester and the control group (p=0.014). However, there was no diffences in the mena platelets larger cell ratio and platelet distribution width in the case and control groups. Fifteen (11.0%) and 7 (12.1%) of the case and control control groups, respectively had mild thrombocytopenia. However, there was no significant association between pregnant status and thrombocytopenia (p=0.836). Based on these findngs, it can be infered that platelet count and MPV decreases while PDW increase with the progression of gestation age compared to the non-pregnant women.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130649909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pigeonpea (Cajanus cajan L.) and Cowpea (Vigna unguiculata L.) Water as Alternative Growth Medium for Escherichia coli and Staphylococcus aureus in Laboratory with Minimum Infrastructure 鸽豆(Cajanus cajan L.)和豇豆(Vigna unguiculata L.)用水作为实验室中大肠杆菌和金黄色葡萄球菌的替代培养基

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2021-10-30 DOI: 10.33086/ijmlst.v3i2.2076

N. Kambuno, Ninda P. Y. Amtaran, Sherly Dewu, K. E. Nurdin, N. Susilawati, Yoan Novicadlitha

{"title":"Pigeonpea (Cajanus cajan L.) and Cowpea (Vigna unguiculata L.) Water as Alternative Growth Medium for Escherichia coli and Staphylococcus aureus in Laboratory with Minimum Infrastructure","authors":"N. Kambuno, Ninda P. Y. Amtaran, Sherly Dewu, K. E. Nurdin, N. Susilawati, Yoan Novicadlitha","doi":"10.33086/ijmlst.v3i2.2076","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.2076","url":null,"abstract":"The availability of non-synthetic media from natural ingredients is needed to answer the needs in laboratories where the price of nutrient media is quite expensive and there are limited supplies of material ware houses. Cowpea (Vigna unguiculata L.) and pigeonpea (Cajanus cajan L.) are the local foods of people of NTT (East Nusa Tenggara) which have a high enough nutritional content which has the potential to be developed into cheap, easy and simple non-synthetic media in making. The purpose of this study was to determine whether the agar media contained nutrient from cowpea and pigeonpea water can be used as a alternative for nutrient agar for the growth of Escherchia coli and Staphylococcus aureus bacteria. This research is a true experiment with posttest-only control design. The growth rate of S. aureus bacteria on pigeonpea medium, cowpea medium, nutrient agar medium, were 164 CFU/mL (SD=3,13), 161 CFU/mL (SD=3,02) and 164 CFU/mL (SD=3,21), respectively. The average growth of E. coli on cowpea medium, pigeonpea medium, and nutrient agar control medium were 163 CFU/mL (SD=2,79), 167 CFU/mL (SD-2,63) and 164 CFU/mL (SD=2,75) respectively. Test results ANOVA between pigeonpea medium, cowpea medium and nutrient medium in order to obtain p value = 0.145 (p> 0.05) for the growth of E. coli bacteria and p value = 0.393 (p> 0.05) for growth S. aureus. It was concluded that there was no difference between the number of bacterial colonies of E. coli and S. aureus on three medium. The pigeonpea medium and cowpea can be used to grow and alternative nutrient agar in order to grow bacteria E. coli and S. aureus.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121190418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Silico Analysis of Antiviral Activity and Pharmacokinetic Prediction of Brazilein Sappan Wood (Caesalpinia sappan L.) Against SARS-CoV-2 Spike Glycoproteins 巴西杉木抗病毒活性的计算机分析及药动学预测抗SARS-CoV-2刺突糖蛋白

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2021-04-27 DOI: 10.33086/IJMLST.V3I1.1854

Dwi Krihariyani, E. Haryanto, Retno Sasongkowati

{"title":"In Silico Analysis of Antiviral Activity and Pharmacokinetic Prediction of Brazilein Sappan Wood (Caesalpinia sappan L.) Against SARS-CoV-2 Spike Glycoproteins","authors":"Dwi Krihariyani, E. Haryanto, Retno Sasongkowati","doi":"10.33086/IJMLST.V3I1.1854","DOIUrl":"https://doi.org/10.33086/IJMLST.V3I1.1854","url":null,"abstract":"Brazilein is one of the secondary sappan wood metabolites which can be used empirically as an antivirus. The SARS-CoV-2 spike (S) glycoproteins play significant roles in attaching and entering the virus into the host cell. This study aims to predict the antiviral activity and pharmacokinetic properties of brazilein of the sappan wood against the in-silico SARS-CoV-2 S glycoproteins with vitamin C as the reference compound. Molegro Virtual Docker 5.5 was used to predict antiviral activity by docking process. SARS-CoV-2 S glycoprotein with NAG ligand available in Protein Data Bank (PDB) (PDB ID: 7C01) was the receptor used. The pkCSM online tool was used to predict the pharmacokinetic properties and toxicity of brazilein. Data were analyzed on the target receptors by comparing the docking bond energies between NAG, brazilein, and vitamin C. The smaller the ligands’ bond energy to the target receptor, the more stable the bonds are. The bond energy of NAG, brazilein, and vitamin C was -59.2864 kcal/mol, -65.8911 kcal/mol, and -53.9093 kcal/mol, respectively. These results suggested that brazilein has a greater capacity as an antivirus compared to NAG and vitamin C. In silico test using the pkCSM online tool demonstrated that brazilein had strong pharmacokinetic properties and relatively low toxicity.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130668042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INVITRO CITOTOXICITY ASSAYS OF SEAGRASS (Enhalus acoroides) METHANOL EXTRACT FROM SOROPIA COASTAL WATERS SOUTHEAST SULAWESI REGENCY 苏拉威西岛东南沿海索罗亚海域海草甲醇提取物体外毒性研究

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2020-04-14 DOI: 10.33086/ijmlst.v2i1.1463

Theosobia Grace Orno, Agnes Rantesalu

{"title":"INVITRO CITOTOXICITY ASSAYS OF SEAGRASS (Enhalus acoroides) METHANOL EXTRACT FROM SOROPIA COASTAL WATERS SOUTHEAST SULAWESI REGENCY","authors":"Theosobia Grace Orno, Agnes Rantesalu","doi":"10.33086/ijmlst.v2i1.1463","DOIUrl":"https://doi.org/10.33086/ijmlst.v2i1.1463","url":null,"abstract":"The studies analysing the use of natural ingredients as an alternative treatment in the field of pharmacology are developing very rapidly. One of researches that is quite promising in the pharmaceutical industry is the application of marine materials. Marine materials that are frequently used consist of shellfish, algae, sponges and seagrass. Several studies on toxicity tests have shown that the methanol extract of seagrass (from species Enhalus acoroides) is more toxic than the other seagrass family. This study aims to test the toxicity level of Seagrass (E. acoroides) extract from Soropia coastal waters. The research method in this study was an experimental laboratory using E. acoroides Seagrass as a sample that was obtained from Soropia Coast, Konawe Regency, Southeast Sulawesi Province. The sample was extracted using methanol as a solvent by macerating it and was tested for its toxicity using the Brine Shrimp Lethality Test (BSLT) method. Toxicity test results showed that the samples with a concentration of 10 ppm, 100 ppm and 1000 ppm in leaves extracts produced an LC50 value of 404.88 ppm, while the stem and root extracts has a value of LC50 >1000 ppm. The test was continued with higher concentration of leaves extracts consisted of 250 ppm, 500 ppm, and 1000 ppm. The toxicity test showed an LC50 value of 0.7309; which means that it was very toxic. The methanol extract of Seagrass (E. Acoroides) is potential to be used for further analysis and anticancer formulations.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121041366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

BLOOD LEAD CONCENTRATIONS AND THE NEUROPSYCHOLOGY SCORES OF PREGNANT WOMEN IN KLANG VALLEY, MALAYSIA 马来西亚巴生谷孕妇血铅浓度与神经心理学评分

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2020-04-09 DOI: 10.33086/ijmlst.v2i1.1499

Shamsul Bahari Shamsudin, J. Hashim, N. Ismail, M. P. Koentjoro

{"title":"BLOOD LEAD CONCENTRATIONS AND THE NEUROPSYCHOLOGY SCORES OF PREGNANT WOMEN IN KLANG VALLEY, MALAYSIA","authors":"Shamsul Bahari Shamsudin, J. Hashim, N. Ismail, M. P. Koentjoro","doi":"10.33086/ijmlst.v2i1.1499","DOIUrl":"https://doi.org/10.33086/ijmlst.v2i1.1499","url":null,"abstract":"Pregnant women with high blood lead posed high risk to their fetus as placental transfer can occurs to the fetus. The objective of this study was to identify the relationship between blood lead and the neuropsychological score of women who were in their 3rd trimester of pregnancy. These respondents were undergoing a routine antenatal checkup at a teaching hospital located in Klang Valley areas. Blood lead concentrations were analyzed using graphite furnace Atomic Absorption Spectrophotometer (AAS). The neuropsychological scores were measured with WHO Neurobehavioral Core Test battery (NCTB). The test consists of 7 items, which made up of the Digit Symbol, Trail Making, Digit Span, Benton Visual Retention Test, Pursuit Aiming, Santa Ana Manual Dexterity, Reaction Time and Movement Time tests. The mean blood lead was 7.78±4.77 µg/dL. The mean score for the total NCTB test was 50.00±5.24. Statistical analysis showed blood lead concentrations were inversely correlated with the total NCTB score (r= –0.462, p≤0.01). The correlation was about 21.3%. The General Linear Model (GLM) showed that age (β= –0.15, p=0.017), weight (β=2.67, p=0.05) and height (β=–1.97, p=0.05) also influence the total neuropsychological scores. In conclusion, blood lead reduces the total neuropsychological scores. The scores for each of the 7 items were inversely and significantly correlated with blood lead concentrations except for the Trail Making and Santa Ana Manual Dexterity tests.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114348018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION OF ERYTHROCYTE SEDIMENTATION RATE (ESR) OF MOTORCYCLE WORKSHOP WORKER EXPOSED TO BENZENE 摩托车车间工人接触苯后红细胞沉降率的评价

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2019-10-18 DOI: 10.33086/ijmlst.v1i2.1303

M. Sahri, M. P. Koentjoro, Shamsul Bahari Shamsudin

{"title":"EVALUATION OF ERYTHROCYTE SEDIMENTATION RATE (ESR) OF MOTORCYCLE WORKSHOP WORKER EXPOSED TO BENZENE","authors":"M. Sahri, M. P. Koentjoro, Shamsul Bahari Shamsudin","doi":"10.33086/ijmlst.v1i2.1303","DOIUrl":"https://doi.org/10.33086/ijmlst.v1i2.1303","url":null,"abstract":"Occupational exposure to benzene from fuel oil in workshop motorcycle workshop causes several health effects, depending upon the level and duration of exposure. Analysis of blood is important to access the status of worker’ health. The study aimed to assess the gather basic information required for protecting workers’ health and improve working conditions in the works sites by investigating the ESR levels. A comparative cross-sectional study conducted in Surabaya City, East Java-Indonesia, which involved 100 workers. The occupational data collected using a structured questionnaire while blood analysis parameters measured with an automated hematology analyzer. The result showed that leukocytes, erythrocytes, hemoglobin (HGB), hematocrit values (HCT), platelet, MCV, RDW, eosinophil, basophils, neutrophil rods, neutrophil segment, lymphocyte, monocytes were in normal value. Whereas, MCH, MCHC, ESR has shown in abnormal value as average 24.5 pg, 29.18 g/dL and 8.21 mm/h, respectively. Those value, especially in ESR value indicated the increasing concentration plasma viscosity. This is may cause by inflammation in the workers’ body. To prevent the hazardous effect of benzene exposure, occupational health should be implemented for workers in order to protect them from exposure to benzene.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126315155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH 100°c水浴甲基化火焰加热本尼迪克特法尿液中葡萄糖还原的比较

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2019-10-18 DOI: 10.33086/ijmlst.v1i2.1075

F. Fadhilah, N. Vanawati

{"title":"COMPARISON OF GLUCOSE REDUCTION IN URINE USING BENEDICT METHOD HEATED BY METHYLATED FLAME WITH 100°C WATERBATH","authors":"F. Fadhilah, N. Vanawati","doi":"10.33086/ijmlst.v1i2.1075","DOIUrl":"https://doi.org/10.33086/ijmlst.v1i2.1075","url":null,"abstract":"The high prevalence of Diabetes Melitus (DM) is a global problem that must be solved by health workers around the world. This study aims to determine the differences in the results of urine reduction examination using benedict method heated by spirtus flame and waterbath 100°C. This research method is a laboratory experiment. The results of this study indicated that the urine reduction examination by heating methylated flame and waterbath 100oC shows the same results from negative (-) until positive (+4). Examination of urine reduction by heating the methylated flame and waterbath 100oC did not affect the results. However, there are a difference in the process of urine reduction examination by heating flame which was need a longer time up to 3-5 minutes. Additionally, the solution in the tube could be exploded. It was also time consuming which could only carry out one by one sample. Meanwhile, the heating of urine by using 100oC waterbath is relatively faster, which only took 2 minutes. The urine was not also exploded when it was boiled and the heating process could perform 6-8 samples at the same time (depending on the tube rack). In conclusion, the heating method of urine by using waterbath was better than spirtus (methanol) flame since it could carry out large sample in one time and it was safer for the laboratory personnel.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"95 20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129160311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE (NT-proBNP) IN STAGE 1 AND STAGE 2 HYPERTENSION PATIENTS 1期和2期高血压患者的n端前脑利钠肽(NT-proBNP)

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2019-10-18 DOI: 10.33086/ijmlst.v1i2.1159

Supriati Wila Djami

{"title":"N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE (NT-proBNP) IN STAGE 1 AND STAGE 2 HYPERTENSION PATIENTS","authors":"Supriati Wila Djami","doi":"10.33086/ijmlst.v1i2.1159","DOIUrl":"https://doi.org/10.33086/ijmlst.v1i2.1159","url":null,"abstract":"The increased levels of NT-proBNP in the blood occur when heart function, especially the left ventricular muscle chambers of the heart increases. Therefore, NT-proBNP is used as a biomarker to detect heart failure.The level of N Terminal – Pro Brain Natriuetic Peptide was independently associated with an increased risk of hypertension. This study aimed to determine the difference of NT-proBNP serum levels and the correlation between the levels of NT-proBNP in patients with stage 1 and stage 2 hypertension. This research was conducted at RSUP dr. Wahidin Sudirohusodo in August - September 2018. The study used a cross-sectional design with the total of 72 hypertensive patients, who had met the inclusive criteria. NT-proBNP levels were measured using the ELISA (Enzyme Linked Immunosorbent Assay) method. The collected data was processed using Mann Whitney Different Test and Spearman's rho Correlation Test. The study results indicated that the level of NT-proBNP in the hypertensive patients with stage 2 was higher and significantly different (p = <0.001) compared to stage 1 hypertensive patients. NT-proBNP levels were higher in the hypertensive group of >6 years than in the hypertensive group <6 years. There were significant differences between the two groups statistically (p=0.010). It can be Conclude that there is a significant difference in the levels of NT-proBNP with a degree of hypertension where NT-proBNP levels were higher in patients with stage 2 hypertension compared to stage 1 hypertension, although there was not statistically significant correlation between levels of NT-proBNP with Hypertension degree. Further research was needed to determine the relationship of NT-proBNP levels with the degree of hypertension, which can confirm the diagnosis, especially in patients with hypertension. Also, it is suggested to consider the accuracy of the data length of a patient suffering from undiagnosed hypertension.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123744469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STUDY OF HEMOGLOBIN LEVELS ON HEMOLYSIS SAMPLE 溶血标本血红蛋白水平的研究

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2019-10-18 DOI: 10.33086/ijmlst.v1i2.1311

N. Febryani, Ikke Nanda Amalia, Intan Dwi Anggraeni, Gilang Nugraha

引用次数: 1

IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY 用碘量法和紫外-可见分光光度法鉴别木瓜中抗坏血酸的含量

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2019-10-18 DOI: 10.33086/ijmlst.v1i2.1291

Nosy Maria Riscahyani, E. Ekawati, Khoirul Ngibad

{"title":"IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY","authors":"Nosy Maria Riscahyani, E. Ekawati, Khoirul Ngibad","doi":"10.33086/ijmlst.v1i2.1291","DOIUrl":"https://doi.org/10.33086/ijmlst.v1i2.1291","url":null,"abstract":"Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128637525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1