Indonesian Journal of Medical Laboratory Science and Technology最新文献

Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks 自制对照血清在-2°~ -4°C和-20°C冷冻保存8周后SGOT和SGPT水平重组的稳定性

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3278

Kadek Profit Hartani, Anik Handayati

{"title":"Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks","authors":"Kadek Profit Hartani, Anik Handayati","doi":"10.33086/ijmlst.v5i1.3278","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3278","url":null,"abstract":"Improving health laboratory services is closely related to strengthening the quality of health laboratories. Serum control is part of the quality assurance of health laboratories. Lyophilized homemade serum is a type of homemade control serum. This research aims to determine the stability of lyophilized homemade control serum against Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels. This research used an experimental method with pooled sera as research material stored at -2°C to -4°C and -20°C for 8 weeks. Pooled sera were obtained from respondents who had no history of disease, were free from HIV, AIDS, and HBsAg. The research was conducted at the Reference Laboratory and Clinical Chemistry Laboratory Politeknik Kesehatan (Poltekkes) Kementerian Kesehatan, Surabaya. The results of the study were data analysis using linear regression test. The SGOT parameter at freezer temperature (-2 to -4°C) showed that the storage time had an effect of 90.97% with an error component of 9.03%. At freezer temperature (-20°C), the result of storage time has an effect of 78.71% with an error component of 21.29%. In the SGPT parameter with freezer temperature (-2 to -4°C), the result of storage time has an effect of 96.71% with an error component of 3.29%. At freezer temperature (-20°C), the result of storage time has an effect of 91.47% with an error component of 8.53%. The results of the SGOT and SGPT examinations did not exceed the limits of ±2SD and ±3SD The CV on the SGOT and SGPT examinations also did not exceed the CCV limits, which is to be stable.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133756331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence of Hepatitis B Surface Antigen (HBsAg) Infection and Its Relationship with Risk Factors at Janitors at Regional General Hospital dr. M. M. Dunda Limboto Gorontalo M. M. Dunda Limboto Gorontalo地区综合医院门卫乙型肝炎表面抗原(HBsAg)感染率及其与危险因素的关系

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3623

Indra Elisabet Lalangpuling, Zumrotul Abidah Dakio, Nikma Nikma, Dwi Setiyo Prihandono, Lendawati Lendawati

{"title":"Incidence of Hepatitis B Surface Antigen (HBsAg) Infection and Its Relationship with Risk Factors at Janitors at Regional General Hospital dr. M. M. Dunda Limboto Gorontalo","authors":"Indra Elisabet Lalangpuling, Zumrotul Abidah Dakio, Nikma Nikma, Dwi Setiyo Prihandono, Lendawati Lendawati","doi":"10.33086/ijmlst.v5i1.3623","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3623","url":null,"abstract":"Hepatitis B is an inflammatory liver disease caused by the Hepatitis B virus, which can be acute or chronic. The outer membrane protein of Hepatitis B Virus (HBV) is known as Hepatitis B Surface Antigen (HBsAg). Janitors are employees who are tasked with cleaning the hospital environment to keep it clean, because of the the dangers that exist in hospitals, such as disease transmission, can occur if the hospital environment is not kept clean. The purpose of this study was to describe the results and their relationship to risk factors of the Hepatitis B examination on janitors at the Regional General Hospital (RSUD) dr. Mansyoer Mohammad Dunda Limboto Gorontalo. This study used a descriptive method with an accidental sampling technique. The sample in this study amounted to 33 respondents who met the inclusion criteria. Specimen were examined using the rapid test method. The data were presented descriptively and statistically to see the relationship between work and the incidence of Hepatitis B infection. The results obtained were as many as 33 respondents by conducting an HBsAg examination, with the results obtained being 2 reactive people and 31 non-reactive people. The results of statistical tests on the relationship between infection status and age showed a p-value of 0.019 and the relationship between infection status and symptoms showed a p-value of 0.002. The study’s findings revealed that there was a substansial correlation between the respondents’ reported symptoms and their infection status (6% of respondents teste for Hepatitis B).","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125678727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk 母乳中干酪乳杆菌群益生菌外多糖的分析

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.2872

Nur Kusmiyati, Y. Puspitasari, U. Utami, Anggeria Oktavisa Denta

{"title":"Analysis of Exopolysaccharides in Lactobacillus casei group Probiotics from Human Breast Milk","authors":"Nur Kusmiyati, Y. Puspitasari, U. Utami, Anggeria Oktavisa Denta","doi":"10.33086/ijmlst.v5i1.2872","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.2872","url":null,"abstract":"Exopolysaccharides get a lot of attention because they can improve the host immune system. Exopolysaccharide is a polysaccharide that is produced and secreted from microbes outside the cell, usually found on the outside of the bacterial structure. The Lactobacillus casei group from human breast milk is thought to have the ability to produce exopolysaccharides. The purpose of the study was to examine the exopolysaccharide of the L. casei group that was isolated from breast milk. The methods used include the gravimetric, the phenol-sulfuric acid and the Fourier Transform Infra-Red (FTIR). The results showed that the L. casei group could produce exopolysaccharides, and had high exopolysaccharide total sugar content. Lactobacillus paracasei had the highest exopolysaccharide and total sugar content of 3660 mg/L and 80.6%, respectively. The FTIR results of the L. casei group exopolysaccharides showed the presence of hydroxyl functional groups O-H (3425.76-3295.98 cm-1), methyl C-H (2930.86-2856.70 cm-1), carbonyl C=O (1660.11-1647.27 cm-1), C-H (1456.16-1373.44 cm-1) and C-O-C ether (1071.08-1056.82 cm-1) which are specific characters of exopolysaccharides. Since the FTIR profile demonstrates that the L. casei group can produce exopolysaccharides, it has greater potential as a a probiotic.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"117 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114327503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Urine Podocalyxin in Type 2 Diabetes Mellitus Subjects With and Without Diabetic Nephropathy 伴有和不伴有糖尿病肾病的2型糖尿病患者尿足霉素的分析

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3933

Jusni Ekasari Pelu, L. B. Kurniawan, Yuyun Widaningsih, Alfian Zainuddin, Husaini Umar, Nurahmi Nurahmi, Theosobia Grace Orno

{"title":"Analysis of Urine Podocalyxin in Type 2 Diabetes Mellitus Subjects With and Without Diabetic Nephropathy","authors":"Jusni Ekasari Pelu, L. B. Kurniawan, Yuyun Widaningsih, Alfian Zainuddin, Husaini Umar, Nurahmi Nurahmi, Theosobia Grace Orno","doi":"10.33086/ijmlst.v5i1.3933","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3933","url":null,"abstract":"Type 2 diabetes mellitus is the most common cause of diabetes, consist from about 85% of cases. Diabetic nephropathy is a complication of diabetes mellitus in the kidneys which can end up as kidney failure. Podocalyxin (PDX) is a protein expressed in kidney podocytes that is involved in various cancers, and is also essential for kidney development. The research design was carried out using observational and cross-sectional analytic methods with total participants of 25 DM with diabetic nephropathy and 25 DM without diabetic nephropathy with a purposive probability sampling technique. This research conducted at the Endocrine Polyclinic, Clinical Pathology Laboratory, Hasanuddin University Medical Research Center (HUM-RC) Laboratory, Hasanuddin University Hospital, Makassar. The results showed that the urinary PDX level in DM subjects with nephropathy were 1.160 ng/mL and DM without nephropathy were 0.167 ng/mL (p<0.001), the urine albumin/creatinine ratio (ACR) of DM subjects with nephropathy were 644.74 mg/ g and DM without nephropathy of 10.071 mg/g (p<0.001) and the correlation test results of urine PDX and urine ACR in DM subjects with nephropathy (r=0.510; p=0.001). This study concluded that there was a significant difference between urinary PDX in DM with and without diabetic nephropathy, there was a significant difference between urine ACR levels in DM with and without diabetic nephropathy, and there was a relationship between urinary PDX levels and urine ACR in DM subjects with diabetic nephropathy.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126228242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymptomatic Bacteriuria and Antibiogram of Isolates Among Diabetic Patients in Calabar, Nigeria 尼日利亚卡拉巴尔地区糖尿病患者无症状菌尿及抗菌谱分析

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3797

P. Inyang-Etoh, E. Etefia, So Ejiofor

{"title":"Asymptomatic Bacteriuria and Antibiogram of Isolates Among Diabetic Patients in Calabar, Nigeria","authors":"P. Inyang-Etoh, E. Etefia, So Ejiofor","doi":"10.33086/ijmlst.v5i1.3797","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3797","url":null,"abstract":"Asymptomatic bacteriuria is the presence of bacteria in the urine of an individual without any symptom of urinary tract infection. This has been widely observed in diabetic patients and could be detrimental to their health if not effectively managed. This study was to identify the pathogens associated with asymptomatic bacteriuria among patients with diabetes and the antibiogram of those isolates in Calabar. Blood samples were collected for the determination of fasting blood sugar levels using glucometer. Bacterial isolations were done through urine culture and antibiogram were tested in all urine samples of the diabetic patients in this study. The prevalence of bacteriuria in this study was 26.0%. Participants aged, 41-50 years were highest both in blood sugar level (12.3+/-4.38mmol/L) and infection rate (37.5%, 18/48). Females had lower blood sugar (10.9+/-3.370mmol/L) than the males (11.3+/-4.46mmol/L) while the males had less infection rate (22.4%, 22/98) than the females (29.4%, 30/102). Escherichia coli, 46.2% (24/52) had the highest distribution while Proteus spp. 11.5% (6/52) had the least distribution. Ciprofloxacin was the most sensitivity (100%) while Amoxicillin was the most resistant (38%). There was a high prevalence of asymptomatic bacteriuria in this study. The most commonly observed organisms were Escherichia coli. Ciprofloxacin was the most sensitive antibiotics and there was a widespread antibiotic resistance in this study. It is therefore recommended that screening among diabetic patients for urinary tract infections, sensitization and strategies to promote effective drug usage be encouraged.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114972699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of APTT Based Clot Waveform Parameters in Various Clinical Conditions – A Study at A Tertiary Care Center 基于APTT的血凝块波形参数在不同临床条件下的分析-一项在三级保健中心的研究

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3064

Rachana Lakhe, A. Nisal, P. Doshi, R. Nimbargi

{"title":"Analysis of APTT Based Clot Waveform Parameters in Various Clinical Conditions – A Study at A Tertiary Care Center","authors":"Rachana Lakhe, A. Nisal, P. Doshi, R. Nimbargi","doi":"10.33086/ijmlst.v5i1.3064","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3064","url":null,"abstract":"Various coagulation tests like Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) are estimated by automated coagulation analyzers. The newer fully automated analyzers generate clot wave forms aPTT-CWA for these parameters are derived. In this study, the objective was to analyze clot wave form characteristics morphology and its first and second derivative values in cases with abnormal APTT. ACL TOP 300 generated curves for APTT in a total 125 patients with 20 normal controls are included. First derivative, second derivative, morphology of curve: sigmoid, biphasic, prolonged pre-coagulation phase, second derivative morphology like early and late shoulder, biphasic peak, delayed deceleration were the analyzed parameters. Wave clot forms of 125 patients were included in this study. Patients (M:F - 2.2:1, mean age: 46.9 ± 20 years). A spectrum of clinical conditions was Covid (20%), liver disease (23%), polytrauma (10.4%), cardiac diseases (8.8%), sepsis/DIC (7.2%), thromboembolism (7.2%), renal diseases (6.4%), bacterial infections (4%), dengue (4%), snake bite (1.6%) and factor deficiency (1.6%). Liver and heart disease showed a significant difference in acceleration and deceleration peaks followed by sepsis, dengue, polytrauma and sepsis/DIC. Deceleration peak was prolonged in patients of Covid (p<0.05). Sepsis and liver diseases showed prolonged first derivative peak (p<0.05). CWA is very easily available on all automated coagulation analyzers. It is inexpensive with fast turn round time. Both quantitative as well as qualitative informations such as velocity, acceleration of clot formation and wave pattern details were recorded. Our study highlights importance of quantitative and qualitative CWA parameters acquired by performing APTT test for the automated analyzers.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123913863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Study of Mean Platelet Volume in Diabetic Population With and Without Vascular Complication 有与无血管并发症的糖尿病人群平均血小板体积的比较研究

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3465

Ajantha Swamy Vasudhevan, Dhivya Mohan Sumathi, Ashwath Kumar Chinnaraju Selvakumar, Rajabalaji Rajabalaji

{"title":"A Comparative Study of Mean Platelet Volume in Diabetic Population With and Without Vascular Complication","authors":"Ajantha Swamy Vasudhevan, Dhivya Mohan Sumathi, Ashwath Kumar Chinnaraju Selvakumar, Rajabalaji Rajabalaji","doi":"10.33086/ijmlst.v5i1.3465","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3465","url":null,"abstract":"Diabetes mellitus leads to long-term complications involving multiple organs and systems of the human body. Among the list of complications, a relatively vascular complication increases the morbidity of the condition. Patients with diabetes mellitus are influenced by various factors like hyperglycaemic state, insulin resistance, oxidative stress, and metabolic condition like obesity, and all the mentioned conditions also present with platelet hyperactivity. Mean platelet volume (MPV) can be used as one of the laboratory parameters to know the function and activation of the platelets, which reflects the vascular profile of the patient. So, the present study compares the values of mean platelet volume among the diabetic groups to determine the relation between the vascular complication and the mean platelet volume. This study was conducted with 90 participants, who were divided into three groups. Group A is non-diabetics, group B is type 2 Diabetics, and Group C is type 2 Diabetics with vascular complications and MPV. On analyzing the statistical mean value of mean platelet volume, group B's (type 2 diabetics) value was higher than group A's (non-diabetics) and statistically significant with a p – value of 0.001. Similarly, the mean value of group C (type 2 diabetes with complications) was higher than group B (type 2 diabetics) and statistically significant with a p – value of 0.049 in the diabetics with and without vascular complications. On comparing the MPV of different study groups, the MPV is higher in the diabetic group with complicatiosn compared with the diabetics without complications.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"120 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133942512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lycopene Improves the Metformin Effects on Blood Glucose and Neutrophil Counts in Type 2 Diabetic Rats 番茄红素改善二甲双胍对2型糖尿病大鼠血糖和中性粒细胞计数的影响

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2023-04-28 DOI: 10.33086/ijmlst.v5i1.3865

Medina Sianturi, N. Susilaningsih, H. Nugroho, Maria Suryani

{"title":"Lycopene Improves the Metformin Effects on Blood Glucose and Neutrophil Counts in Type 2 Diabetic Rats","authors":"Medina Sianturi, N. Susilaningsih, H. Nugroho, Maria Suryani","doi":"10.33086/ijmlst.v5i1.3865","DOIUrl":"https://doi.org/10.33086/ijmlst.v5i1.3865","url":null,"abstract":"In patients with type 2 diabetes mellitus (T2DM), both innate and acquired immunity are weakened by hyperglycemia. Lycopene is one of the hydrocarbon carotenoids that has been widely studied for its powerful antioxidant and anti-inflammatory properties, furthermore act as hypoglycemic and immunomodulator. Herein, we investigated the effect of lycopene and metformin combination on fasting blood glucose (FBG) and neutrophil counts. The rats were divided randomly into six groups, each containing five rats. Group 1 consisted of normal rats (N) and group 2, T2DM (DM) rats, which were administered 0.5 mL of coconut oil; group 3 T2DM rats were administered 250 mg/kg of metformin in 0.5 mL of coconut oil; groups 4,5 and 6 rats were administered a combination of metformin 250 mg/kg with 10 mg/kg (DML-10), 20 mg/kg (DML-20) and 40 mg/kg (DML-40) of lycopene in 0.5 mL of coconut oil, respectively. Treatment was administered every day for 28 days. A model of T2DM rats was induced by a high-fat diet for two weeks combined with streptozotocin–nicotinamide. Data were analyzed with a one-way ANOVA test followed by the least significant difference (LSD) test. There were significant differences in FBG levels and the number of neutrophils in all groups. Lycopene combined with metformin had lower FBG concentrations and higher neutrophil counts compared to metformin monotherapy (p<0.001), and these observations were dose-dependent. Lycopene combined with metformin can improve blood glucose and neutrophil counts in rats with diabetes. The highest effect was observed in combination with lycopene at a dose of 40 mg/kg and metformin at a dose of 250 mg/kg.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132872641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Bay Leaf (Syzygium polyanthum) Extract on Antioxidant Activity, MDA Levels, and Liver Histopathology Feature of Ethambutol Induced Wistar Rats 月桂叶提取物对乙胺丁醇诱导Wistar大鼠抗氧化活性、丙二醛水平及肝脏组织病理学特征的影响

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.2471

B. E. Suwito, Lea Maera Shanty, Retna Gumilang, H. Handayani, R. Ulhaq

{"title":"Effect of Bay Leaf (Syzygium polyanthum) Extract on Antioxidant Activity, MDA Levels, and Liver Histopathology Feature of Ethambutol Induced Wistar Rats","authors":"B. E. Suwito, Lea Maera Shanty, Retna Gumilang, H. Handayani, R. Ulhaq","doi":"10.33086/ijmlst.v4i2.2471","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.2471","url":null,"abstract":"Bay leaf extract (Syzygiun polianthum) is one herbal elemnt that may be used to lessen liver function issues, lessen symptops of nausea, vomiting, discomfort and improve adherence and the effectiveness of tuberculossi treatment. The objective of this study was to determine the effect of bay leaf (Syzygium polyanthum) extract on antioxidant activity, Malondialdehyde (MDA) levels, and liver histopathology of ethambutol-induced Wistar rats. This research is a laboratory experiment. Antioxidant activity of Bay Leaf Extract was evaluated by comparing vitamin C with spectrophotometry methods. White Wistar rats were separated into 6 groups and uset to test the lebel of MDA and livers histopathology. Group 1 serves as the control group and received DMSO (placebo); Group 2 was received ethambutol 50mg/kg BW; Group 3 was received ethambutol and silymarine with dose 50mh/kg BW. Group 4-6 had been given ethambutol and extra ethanol extract of bay leaves of 75,150, dan 300mg/kg BW, respectively. The results of the spectrophotometry showed that the Bay Leaf Extract had antioxidant activity comparable to that of vitamin C, with an IC50 of 11.4 g ± (4.4%). One-Way ANOVA test results obtained p = 0.002 (p<0.05) while the Kruskal-Wallis test results obtained the Asymp. Sig value 0.105> 0.05. There was a significance difference in each group’s MDA levels (p=0.002). Although there was no significant difference in the liver histopathology of treated rats (p>0.05). While bay leaf extract significantly lowers MDA levels in ethambutol-induced Wistar rats, it has no discernible impact on the liver histopathology of ethambutol-induced Wistar rats. Bay leaf extract possesses antioxidant activity comparable to vitamin C.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"2018 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121537257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Effect of Cigarette Smoking Duration on Hemoglobin Level Measured with Cyanmethemoglobin Method 吸烟时间对氰高铁血红蛋白法测定血红蛋白水平的影响

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.2674

Fitriani Kahar, Ririh Jatmi Wikandari, I. Irnawati, Martha Silpa Penmaley

{"title":"The Effect of Cigarette Smoking Duration on Hemoglobin Level Measured with Cyanmethemoglobin Method","authors":"Fitriani Kahar, Ririh Jatmi Wikandari, I. Irnawati, Martha Silpa Penmaley","doi":"10.33086/ijmlst.v4i2.2674","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.2674","url":null,"abstract":"In Indonesia, cigarette consumption is quite prevalent and has been become more common over time. According to Riskesdas (Baseline Health Research from Agency of Health Research and Development) study in 2018, the national percentage of people aged who smoke increased from 2016-2018, namely from 3.39% in 2016, 3.9% in 2017 and 9.65% in 2018. Smoking habits will negatively affect human health. Cigarette smoke contains various of different chemicals that are harmful to health, including nicotine addiction, tar (carcinogen or cancer-causing), and carbon monoxide. When inhaled, they form carboxyhemoglobin with Hb (Hemoglobin). When this occurs, the amount of Hb available for oxygen transport is reduced. By way of compensation, the body will produce more red blood cells. The objective of the study was to determine the hemoglobin levels in smokers with smoking durations of 3 years, 4 years and 5 years. This study is quasi-experimental. Determine hemoglobin readings using a photometer 5010 with the Cyanmethemoglobin method. Up to 20 samples were analyzed with SPSS version 20 with Anova analysis at 95% confidence level. The results of statistical analysis showed p-value <0.05, which means that there is a significant effect between variables and vice versa. Hb level comparison between durations of smoking are of 3 years with an average value of 14.8, Hb levels of 4 years with an average value of 14.53, and Hb levels of 5 years with an average value 15.94. The results showed that there was an effect of smoking duration on the results of the examination of hemoglobin levels in smokers of 3,4 and 5 years with the greatest effect being smokers with a duration of 5 years. The duration of smoking impact on hemoglobin levels.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123941137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0