苏拉威西岛东南沿海索罗亚海域海草甲醇提取物体外毒性研究

Theosobia Grace Orno, Agnes Rantesalu
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引用次数: 1

摘要

在药理学领域,分析利用天然成分作为替代疗法的研究正在迅速发展。海洋材料的应用是医药工业中很有前途的研究之一。经常使用的海洋材料包括贝类、藻类、海绵和海草。几项关于毒性测试的研究表明,海草(来自Enhalus acoroides物种)的甲醇提取物比其他海草家族的毒性更大。本研究旨在检测索罗亚沿海海域海草提取物的毒性水平。本研究采用室内实验方法,以苏拉威西省东南部科纳维县索罗亚海岸采集的acoroides海草为样品。样品以甲醇为溶剂浸渍提取,并采用卤虾致死试验(BSLT)法检测其毒性。毒性试验结果表明,叶片提取物中浓度为10 ppm、100 ppm和1000 ppm的样品产生的LC50值为404.88 ppm,而茎和根提取物的LC50值>1000 ppm。以250 ppm, 500 ppm和1000 ppm的更高浓度的叶提取物继续试验。毒性试验LC50值为0.7309;也就是说它的毒性很大。海草(E. Acoroides)甲醇提取物在进一步分析和抗癌制剂中具有潜在的应用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
INVITRO CITOTOXICITY ASSAYS OF SEAGRASS (Enhalus acoroides) METHANOL EXTRACT FROM SOROPIA COASTAL WATERS SOUTHEAST SULAWESI REGENCY
The studies analysing the use of natural ingredients as an alternative treatment in the field of pharmacology are developing very rapidly. One of researches that is quite promising in the pharmaceutical industry is the application of marine materials. Marine materials that are frequently used consist of shellfish, algae, sponges and seagrass. Several studies on toxicity tests have shown that the methanol extract of seagrass (from species Enhalus acoroides) is more toxic than the other seagrass family. This study aims to test the toxicity level of Seagrass (E. acoroides) extract from Soropia coastal waters. The research method in this study was an experimental laboratory using E. acoroides Seagrass as a sample that was obtained from Soropia Coast, Konawe Regency, Southeast Sulawesi Province. The sample was extracted using methanol as a solvent by macerating it and was tested for its toxicity using the Brine Shrimp Lethality Test (BSLT) method. Toxicity test results showed that the samples with a concentration of 10 ppm, 100 ppm and 1000 ppm in leaves extracts produced an LC50 value of 404.88 ppm, while the stem and root extracts has a value of LC50 >1000 ppm. The test was continued with higher concentration of leaves extracts consisted of 250 ppm, 500 ppm, and 1000 ppm. The toxicity test showed an LC50 value of 0.7309; which means that it was very toxic. The methanol extract of Seagrass (E. Acoroides) is potential to be used for further analysis and anticancer formulations.
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