IDENTIFICATION OF ASCORBIC ACID CONTENT IN Carica papaya L. USING IODIMETRY AND UV-VIS SPECTROPHOTOMETRY

Nosy Maria Riscahyani, E. Ekawati, Khoirul Ngibad
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引用次数: 1

Abstract

Ascorbic acid mostly comes from vegetables and fruits, especially fresh fruits. Vitamin C is a vitamin that can be formed by several types of plants. One of them is papaya which has various contents including vitamin C that can increase endurance, help skin rejuvenation and repair body tissues. The purpose of this study was to determine the levels of vitamin C contained in papaya using iodimetry and UV-Vis spectrophotometry. The sample used in this study was papaya fruit. Determination of vitamin C levels in papaya samples using the titration method bas done by adding sample filtrate with starch indicator then titrated with titrant I2 until the endpoint color of blue titration is formed. Other hands, the determination of vitamin C levels in papaya samples using the UV-Vis spectrophotometry method bas conducted by making an ascorbic acid calibration curve then the filtrated sample was added with H2SO4 5% and ammonium molybdate reagent, then the absorbance of the sample was measured at 494 nm wavelength. The results of the determination of vitamin C levels using the iodimetry was 0.0147% and the results of the determination of vitamin C levels using the UV-Vis spectrophotometry method was 0.1313%. In conclusion, vitamin C levels analyzed by using UV-Vis spectrophotometry methods were greater than vitamin C levels analyzed by using the iodimetry method.
用碘量法和紫外-可见分光光度法鉴别木瓜中抗坏血酸的含量
抗坏血酸主要来自蔬菜和水果,尤其是新鲜水果。维生素C是一种可以由几种植物合成的维生素。其中之一是木瓜,它含有多种成分,包括维生素C,可以增加耐力,帮助皮肤恢复活力和修复身体组织。本研究采用碘量法和紫外-可见分光光度法测定木瓜中维生素C的含量。在这项研究中使用的样本是木瓜果实。用本方法测定木瓜样品中维生素C的含量,先用淀粉指示剂加入样品滤液,再用滴定剂I2滴定,直至形成蓝色滴定终点。另一方面,用紫外可见分光光度法测定木瓜样品中维生素C的含量,通过制作抗坏血酸校准曲线,然后将过滤后的样品加入5% H2SO4和钼酸铵试剂,然后在494 nm波长处测量样品的吸光度。碘量法测定维生素C含量的结果为0.0147%,紫外可见分光光度法测定维生素C含量的结果为0.1313%。综上所述,紫外-可见分光光度法测定的维生素C含量高于碘量法测定的维生素C含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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