Pigeonpea (Cajanus cajan L.) and Cowpea (Vigna unguiculata L.) Water as Alternative Growth Medium for Escherichia coli and Staphylococcus aureus in Laboratory with Minimum Infrastructure

N. Kambuno, Ninda P. Y. Amtaran, Sherly Dewu, K. E. Nurdin, N. Susilawati, Yoan Novicadlitha
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Abstract

The availability of non-synthetic media from natural ingredients is needed to answer the needs in laboratories where the price of nutrient media is quite expensive and there are limited supplies of material ware houses. Cowpea (Vigna unguiculata L.) and pigeonpea (Cajanus cajan L.) are the local foods of people of NTT (East Nusa Tenggara) which have a high enough nutritional content which has the potential to be developed into cheap, easy and simple non-synthetic media in making. The purpose of this study was to determine whether the agar media contained nutrient from cowpea and pigeonpea water can be used as a alternative for nutrient agar for the growth of Escherchia coli and Staphylococcus aureus bacteria. This research is a true experiment with posttest-only control design. The growth rate of S. aureus bacteria on pigeonpea medium, cowpea medium, nutrient agar medium, were 164 CFU/mL (SD=3,13), 161 CFU/mL (SD=3,02) and 164 CFU/mL (SD=3,21), respectively. The average growth of E. coli on cowpea medium, pigeonpea medium, and nutrient agar control medium were 163 CFU/mL (SD=2,79), 167 CFU/mL (SD-2,63) and 164 CFU/mL (SD=2,75) respectively. Test results ANOVA between pigeonpea medium, cowpea medium and nutrient medium in order to obtain p value = 0.145 (p> 0.05) for the growth of E. coli bacteria and p value = 0.393 (p> 0.05) for growth S. aureus. It was concluded that there was no difference between the number of bacterial colonies of E. coli and S. aureus on three medium. The pigeonpea medium and cowpea can be used to grow and alternative nutrient agar in order to grow bacteria E. coli and S. aureus.
鸽豆(Cajanus cajan L.)和豇豆(Vigna unguiculata L.)用水作为实验室中大肠杆菌和金黄色葡萄球菌的替代培养基
需要从天然成分中获得非合成培养基,以满足实验室的需求,因为这些实验室的营养培养基价格相当昂贵,而材料仓库的供应有限。豇豆(Vigna unguiculata L.)和鸽豆(Cajanus cajan L.)是NTT(东努沙登加拉)人民的地方食物,营养成分足够高,具有发展成为廉价、简便、简易的非合成培养基的潜力。本研究的目的是确定含有豇豆和鸽豆水营养成分的琼脂培养基是否可以作为大肠杆菌和金黄色葡萄球菌生长的营养琼脂替代品。这项研究是一个真正的后测控制设计实验。金黄色葡萄球菌在木豌豆培养基、豇豆培养基和营养琼脂培养基上的生长速率分别为164 CFU/mL (SD=3,13)、161 CFU/mL (SD=3,02)和164 CFU/mL (SD=3,21)。大肠杆菌在豇豆培养基、鸽豆培养基和营养琼脂对照培养基上的平均生长量分别为163 CFU/mL (SD=2,79)、167 CFU/mL (SD-2,63)和164 CFU/mL (SD=2,75)。结果对鸽豆培养基、豇豆培养基和营养培养基进行方差分析,得出大肠杆菌生长的p值= 0.145 (p> 0.05),金黄色葡萄球菌生长的p值= 0.393 (p> 0.05)。结果表明,大肠杆菌和金黄色葡萄球菌在三种培养基上的菌落数量没有差异。鸽豆培养基和豇豆培养基可以用来培养大肠杆菌和金黄色葡萄球菌,也可以用来培养替代营养琼脂。
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