{"title":"Severe Anemia and Six-Month All-Cause Mortality in Chronic Kidney Disease Patients Undergoing Hemodialysis","authors":"Langgeng Perdhana, Shofa Chasani","doi":"10.33086/ijmlst.v4i1.1963","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.1963","url":null,"abstract":"Anemia is often found in patients with Chronic Kidney Diseases (CKD). Anemia can affect poor outcomes in hemodialysis patients. However, no publications examined the role of severe anemia as a predictor factor in six-month all-cause mortality in hemodialysis patients. Therefore, further study was needed to answer these questions. This study aimed to determine the role of severe anemia and six-month all-cause mortality in hemodialysis patients. The cohort design study was carried out from October 2019 to March 2020 at the Hemodialysis Unit of Roemani Muhammadiyah Hospital Semarang. The dependent variable was severe anemia, defined as hemoglobin levels <8.0 g/dL. The mortality rate was investigated over six months after the baseline measurements. Among 85 respondents, 35 (41.2%) respondents were categorized into severe anemia, and 50 (58.8%) respondents were categorized into non-severe anemia groups. The respondents consisted of 54 (63.5%) males and 31 (36.5%) females. The hemoglobin level in the severe and non-severe anemia groups was 7.2 ± 0.6 and 9.5 ± 1.2 (mean ± standard deviation). The Kaplan Meier survival curve showed that the non-severe anemia group had a higher survival rate than the severe anemia group (98.0% vs. 82.9%, p = 0.011). The Cox regression analysis found a significant relationship between anemia severity and 6-month all-cause mortality in hemodialysis patients (p = 0.027; Hazard ratio: 9.3). Severe anemia plays a role as a predictor factor in six-month all-cause mortality among hemodialysis patients.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116574769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Azzaubadilluah Azzaubadilluah, Andreas Putro Ragil Santoso
{"title":"The Relationship between Hemoglobin Levels and Erythrocyte Morphology on the Third Day of Menstruation for Students in The Faculty of Health","authors":"Azzaubadilluah Azzaubadilluah, Andreas Putro Ragil Santoso","doi":"10.33086/ijmlst.v4i1.2461","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2461","url":null,"abstract":"Menstruation is the process of releasing the endometrial lining of the uterus due to the release of the hormones estrogen and progesterone, causing bleeding after ovulation from the uterus for approximately fourteen days at regular intervals. The first three days of menstruation is a condition where there is heavy bleeding, so there is iron loss, which can cause a decrease in hemoglobin levels and there is a risk of causing anemia. The occurrence of anemia gives an illustration of morphological changes of erythrocytes. The objective of this study was to determine the relationship between hemoglobin levels and erythrocyte morphology on the third day of menstruation. This type of research is analytic observational with a cross-sectional approach. The sample in this study were 30 students from the Health Faculty of Nahdlatul Ulama University Surabaya who experienced menstruation on the third day. The hemoglobin level was analysed using the Hematology Analyzer Xp-300 method. A blood smear test with Giemsa stain were used for abnormalities erythrocytes morphology observation. The results of the statistical analysis used the chi-square test showed there was no relationship between hemoglobin levels and erythrocyte size (p-value = 0.836), erythrocyte shape (p-value = 0.416). However, there was a relationship between hemoglobin levels and erythrocyte colour (p<0.05). Based on the study result, it can be concluded that there is no relationship between hemoglobin levels and erythrocyte morphology on the third day of menstruation.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133240516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Syahrini, T. H. Fadjari, Nadjwa Zamalek Dalimoenthe
{"title":"Neutrophil-Lymphocyte Ratio (NLR) and Lymphocyte-Monocyte Ratio (LMR) as Covid-19 Screening Parameters","authors":"H. Syahrini, T. H. Fadjari, Nadjwa Zamalek Dalimoenthe","doi":"10.33086/ijmlst.v4i1.2281","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2281","url":null,"abstract":"Covid-19 diagnosis generally uses RT-PCR as the gold standard to detect coronavirus-2 (SARS-CoV-2); however, this method requires advanced laboratory equipment. Alternatively, Neutrophil-Lymphocyte Ratio (NLR) and Lymphocyte-Monocyte Ratio (LMR) can be used to identify viral infection. The study aimed: (1) to compare each NLR and LMR ratio in patients with and without COVID-19 and (2) to test the effectiveness of these ratios in identifying COVID-19. The study was conducted at the Haji Adam Malik Central General Hospital by acquiring 87 medical records data. The complete hematologic profile was analyzed from patients with and without COVID-19. The NLR and LMR ratio accuracy were analyzed as a screening tool for COVID-19. The AUC of NLR was 0.638, with cut-off ≤ 2.49, 47.6% sensitivity, and 80% specificity; therefore, the NLR accuracy as a screening for COVID-19 was defined as not good (just sufficient) because of AUC <0,7. The AUC of LMR was 0.661, with cut-off ≥ 3.23, 45.2% sensitivity, and 82.2% specificity; therefore, the LMR accuracy as a screening parameter for COVID-19 is defined as not good (just sufficient) because of AUC <0,7. There were significant differences in hematologic profile in neutrophil, lymphocyte, NLR, LMR between the patients in the COVID-19 group and non-COVID-19 group. NLR and LMR cannot be used as a screening tool because the Area Under Curve (AUC) is not good enough (just sufficient) in detecting COVID-19.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132648526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dela Yorike, Muhammad Rizki Kurniawan, Mohamad Syafaat
{"title":"Analysis of D-Dimer Level and Prothombin Time (PT) Activated Prothombin Thromboplastin (APTT) on Heparin Administration to COVID-19 Patients","authors":"Dela Yorike, Muhammad Rizki Kurniawan, Mohamad Syafaat","doi":"10.33086/ijmlst.v4i1.2487","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2487","url":null,"abstract":"Coronavirus Disease 2019 (COVID-19) is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). Coronavirus is a dangerous pathogen that affects both humans and animals. Symptoms and indicators of COVID-19 infection include fever, cough, and shortness of breath, which are common in respiratory illnesses. COVID-19 has spread globally with high mortality and morbidity rates. Prothrombin Time (PT), Activated Prothrombin Thromboplastin (APTT), and D-Dimer parameters are blood tests to evaluate the coagulation status of COVID-19 patients, all these parameters increased in COVID-19 patients. This study aims at determininig the levels of D-Dimer, PT, and APTT in COVID-19 patients. Clinical and laboratory records were retrospectively reviewed from 100 cases of COVID-19 admitted to hospitals. This study used a a cross-sectional, descriptive, secondary data analysis design. The sample included patients from the Bhayangkara TK. I R.Said Sukanto Hospital between April-July 2021. The sample is a COVID-19 patient who was treated in the Intensive Care Unit (ICU), worsened, and given heparin. An independent variable is the administration of intravenous heparin at prophylactic doses. Dependent variables are D-Dimer, PT, and APTT values. The collected data is processed using Microsoft Office Excel and then being analyzed. D-Dimer, PT, and APTT levels in COVID-19 patients were initially high or increased after heparin administration decreased or became normal. In conclusion, there was a decrease in D-dimer levels, PT values, and APTT after administration of intravenous heparin at prophylactic doses.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123724923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ainul Rofik, Utami Ambarsari, Hafizh Auliyan Sodali, M. Munir
{"title":"The Relationship Between Leukocytes Numbers and Consciousness Level of Craniotomy Patients at The Jemursari Islamic Hospital Surabaya in 2018-2019","authors":"Ainul Rofik, Utami Ambarsari, Hafizh Auliyan Sodali, M. Munir","doi":"10.33086/ijmlst.v3i2.1889","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.1889","url":null,"abstract":"This study investigates the correlation between leukocyte number and the patient's awareness level after craniotomy surgery, both high (leukocytosis) and low (leukopenia). Several studies reveal that an increase in leukocytes affects mortality rates due to the high level of leukocytes affecting our body's functionalities, including awareness. Previous studies revealed that a leukocyte count exceeding 17.5x106/L was associated with a lower GCS score, a longer hospital stay, and worsening CT scan results, regardless of the type of focal lesion that occurred. This study uses observational methods in the form of retrospective case studies. The data was collected from the medical records of the Operation room at Jemursari Islamic Hospital in 2018-2019 with a sample size of 89. The research was conducted at Jemursari Islamic Hospital Surabaya from May 2018 to August 2019. Data were analyzed using the Spearman correlation test with p <0.05. Results showed no significant relationship between the leukocyte count and the consciousness level of post-craniotomy patients. Future research could estimate the specific effects on the morphology of specific leukocyte cells that are elevated in post-craniotomy patients. This research is expected to provide insight into the effect of leukocytes on consciousness to reduce patient mortality after craniotomy.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127258293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modified Spin Column-Based RNA Extraction Methods of Staphylococcus aureus using PureLink® RNA Mini Kit and Basic Laboratory Instrument","authors":"M. T. Hidayat, E. Prasetyo","doi":"10.33086/ijmlst.v3i2.1863","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.1863","url":null,"abstract":"RNA extraction is an important process before gene expression assessment at the transcriptomic level. RNA is a sensitive material to environmental factors such as temperature and contaminants, so the RNA extraction process generally requires sophisticated and expensive laboratory instruments. In this study, we extract RNA from Staphylococcus aureus bacteria using the PureLink® RNA Mini Kit. The instruments used in this study are basic instruments such as a hand homogenizer and non-thermal centrifuge. The results of RNA extraction were visualized using agarose gel electrophoresis. These results indicate that bacterial RNA extraction can be performed using the PureLink® RNA Mini Kit even with inexpensive basic laboratory instruments.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133619326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emma Ismawatie, Purwanto Adhipireno, Seso Sulijaya Suyono, E. Purwanto, Budi Santoso, E. K. S. Limijadi
{"title":"Differences of Spermatozoa Concentration Analysis Between Manual and Automatic Methods","authors":"Emma Ismawatie, Purwanto Adhipireno, Seso Sulijaya Suyono, E. Purwanto, Budi Santoso, E. K. S. Limijadi","doi":"10.33086/ijmlst.v3i2.1961","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.1961","url":null,"abstract":"The examination of sperm concentration in the laboratory is the calculation of the number and motility using a microscope or using a device. There are still some clinicians who doubt the accuracy of the sperm count results using a semen analyzer rather than using the manual method. This study aims is to determine the differences of the sperm concentration examination between the manual method and the automatic method. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA \"Restu Ibu\" Sragen from June to August 2020. The object of this research is the examination of sperm concentration, using a manual method using a hemocytometer and an automatic method using the LensHooke ™ SQA X1 Pro. The results of statistical tests using the Mann Whitney methods show that the significance value (p) was 0.960, which means that there was no difference in the results of the sperm concentration examination between the manual method and the automatic method. Result of this research shows that there is no weakness or significant difference if compared between manual and automatic methods.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129196941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Electrolytes Levels (Na, K, Cl) in Serum Stored at 4°C Temperature","authors":"Ama Fauziah, M. A. Martsiningsih, B. Setiawan","doi":"10.33086/ijmlst.v3i2.1870","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.1870","url":null,"abstract":"The samples used for serum electrolyte measurement should be analyzed immediately after being received in the laboratory within 1-2 hours to avoid an increase in the error of the results. Serum should be stored at 4°C for a period to prevent damage. The analyst should consider maximum delay time in the examination to maintain the serum's quality. This study compared the 2-hour and 3-hour delays in sodium (Na), potassium (K), and chlorine (Cl) tests. The method used in this study is an observational analysis with a cross-sectional study design. The samples in this study used 35 patient serum residues. The study was conducted in November 2020 with a continuous sampling technique. Electrolyte levels in the sample were measured by AVL 9180 Electrolyte Analyzer using Ion-Selective Electrode (ISE) method. The differences in electrolyte (Na, K, Cl) levels were analyzed by the Kruskal-Wallis Statistical test at a 95% confidence level. The results showed that the content of sodium, potassium, and chlorine were 0.719; 0.976; and 0.772. This study showed that there was no significant difference in the electrolyte content of sodium (Na), potassium (K), and chlorine (Cl) in the serum directly detected from the serum stored at 4°C for 2 hours and 3 hours. In conclusion, it is acceptable to postpone the serum test for 3 hours with various considerations.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134602200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Choirotussanijjah Choirotussanijjah, Harianto Notopuro, E. Qurnianingsih
{"title":"Effects of Ethanol Extract of Rosa damascene on HbA1c Level and NF-κB Expression in Diabetic Rats","authors":"Choirotussanijjah Choirotussanijjah, Harianto Notopuro, E. Qurnianingsih","doi":"10.33086/ijmlst.v3i2.2151","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.2151","url":null,"abstract":"Diabetes mellitus (DM) is a chronic hyperglycemic that can cause complications in several organs. It could lead glycosylated hemoglobin or HbA1c formation which has ability undergo further changes into Advanced Glycation End Products (AGEs) and stimulate activation of transcription factors such as nuclear factor kappa B (NF-κB). Some studies showed that anthocyanin has antioxidant and anti-inflammatory activity and it has been found Rosa damascene contain high level of anthocyanin. Total anthocyanin content was 0.459 ± 0.003 mg/L. The aim of this study was to investigate inhibitory effect of R. damascena extract on HbA1c levels and NF-κB activation in diabetic rats. Male wistar rats (n=24) were divided into 6 groups as normal control (KN), streptozotocin-induced diabetic control (KP), diabetic treated with R. damascene (P1, P2, P3; 250, 500 and 1000 mg/kg/d respectively) and diabetic treated with metformin (KM; 500 mg/kg/d). This was carried out for 28 days. The inhibition mode of R. damascene extract was examined by measuring HbA1c levels and expression of NFκB by immunohistochemistry. The results showed p values > 0.05 for HbA1c and p < 0.05 for NFκB. From immunohistochemical staining, it seen the expression of NFκB was low in treated group (P1, P2, P3 and KM) compared with KP. Thus, oral administration of R. damascene extract for 28 days could not reduce HbA1c levels, but can supress NFκB expression.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125586480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Factors Related to Soil Transmitted Helminth Infection in Vegetable Farmers","authors":"Ririh Jatmi Wikandari, Lilik Setyowatiningsih, M. Djamil, Surati Surati, Fitriani Kahar","doi":"10.33086/ijmlst.v3i2.2145","DOIUrl":"https://doi.org/10.33086/ijmlst.v3i2.2145","url":null,"abstract":"Soil Transmitted Helminth (STH) infection can occur at all ages, from children to the elderly. Farmers are at risk of contracting STH infection because of their daily work which is in direct contact with the soil. The risk of STH infection is due to poor personal hygiene and environmental sanitation. The study aims to determine observe the factors associated with STH infection in vegetable farmers, using a cross-sectional approach. The research subjects were vegetable farmers in Batur Wetan Hamlet, Getasan, Semarang Regency. Data were collected using a questionnaire to determine the personal hygiene and sanitation hygiene of farmers when working in the garden. Worm identification used (reference,) based on the worm performance using the floating method. Worm identification were done by examining the stool using the floating method. The results of the study were processed using SPSS version 20. Data analysis using Chi-Square (α = 5%), found 3 positive study subjects infected with STH, consisting of 1 egg of Ascaris lubricoides and 2 eggs of Trichuris trichiura. Positive STH respondents, have a washing by water and soap habits before eating and defecating, without wearing gloves. There was a relationship between hand washing by water and soap habits before eating to worm infection, but no relationship between this habit after defecating and no wearing gloves to worm infection. Vegetable farmers are advised to change the daily habits and maintain personal hygiene after working. use personal protective equipment when working and maintain personal hygiene.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"77 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134285227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}