Indonesian Journal of Medical Laboratory Science and Technology最新文献_第2页

Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples 大蒜滤液作为全血抗凝剂的潜力

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.2683

Ari Nuswantoro, Jessica Ningtyas Berlianti

{"title":"Potential of Garlic Filtrate as An Alternative Anticoagulant for Whole Blood Samples","authors":"Ari Nuswantoro, Jessica Ningtyas Berlianti","doi":"10.33086/ijmlst.v4i2.2683","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.2683","url":null,"abstract":"Synthetic anticoagulants such as heparin, citric, and ethylenediaminetetraacetic acid (EDTA) are commonly used to prevent blood clots. In contrast, its widespread use in clinical laboratories is still constrained by price, its toxic nature, and its short shelf life. Therefore, an alternative material that is relatively cheap, non-toxic, and easy to obtain and process in a ready-to-use form is needed. Garlic contains allicin and ajoene, which are anti-platelet and anti-thrombogenic. This study’s aim is to explore the potential of garlic filtrate as an alternative anticoagulant. Blood from 16 individuals was used and separated into four groups: non-anticoagulant, 50 μL/mL garlic filtrate, 100 μL/mL garlic filtrate, and 150 μL/mL heparin, for a total of 64 treatments. The Lee and White method showed that non-anticoagulated blood had normal clotting times (mean 8 minutes and 56 seconds), whereas heparin plasma and garlic filtrate plasma had longer clotting times (more than 20 minutes); and this is statistically different based on the ANOVA test with a significance value (p) of 0.000 < 0.05. On spectrophotometric measurements, the levels of calcium ions in heparinized plasma and serum were 8.66 mg/dL and 8.52 mg/dL, respectively, while in garlic plasma filtrate of 50 μL/mL and 100 μL/mL were 4.13 mg/dL and 3.58 mg/dL, respectively; this is also statistically different based on the ANOVA test with a significance value of 0.000 < 0.05. The differences indicate that garlic filtrate can extend clotting time and reduce calcium ions therefore it is worth reviewing as an alternative anticoagulant.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125551820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C 37℃和25℃条件下肺炎克雷伯菌生长抑制带直径的差异

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.2919

Juwy Trianes, B. Bastian, Dewi Hartati

{"title":"Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37°C and 25°C","authors":"Juwy Trianes, B. Bastian, Dewi Hartati","doi":"10.33086/ijmlst.v4i2.2919","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.2919","url":null,"abstract":"Pneumonia is an infection that causes the largest death in children worldwide (South Sumatra Province), which occupies the 6th position. One of the laboratory re-identification of Klebsiella pneumonia is a multi-antibiotic testing. The high sensitivity antibiotic that is still used is amikacin. The uniqueness of the diameter of the inhibition zone using the antibiotic amikacin is that it is active against most gram-negative bacilli. But one of the several factors that affect the diameter of the inhibition zone is the incubation temperature. The optimum temperature for pathogenic bacteria is 37oC by using an incubator; however, several factors in the use of instruments such as frequent instability and disruption of installation lead to a need of incubation at 25oC. The study aimed to determine the difference in the diameter of the growth inhibition zone of K. pneumoniae after incubation at 37oC and 25oC. This research is an experimental research conducted at the Microbiology Laboratory of IKesT Muhammadiyah Palembang. The sample is K. pneumoniae which will be subjected to gram staining, biochemical tests, followed by a sensitivity test on Mueller Hinton media which is given an amikacin antibiotic disk and incubated at 370C and 250C in order to calculate the diameter of the zone of inhibition for the growth of K. pneumoniae bacteria. The data was analyzed using the alternative Wilcoxon test which obtained a p value of 0.014. The results of this investigation showed that K. pneumoniae incubated at 37°C and 25°C had a significantly different diameter of the growth inhibition zone.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123008429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of Rapid Antibody (IgM – IgG) Test Kit for SARS-CoV-2 Infection in Surabaya, Indonesia 印度尼西亚泗水市SARS-CoV-2感染快速抗体(IgM - IgG)检测试剂盒的验证

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.3027

Museyaroh Museyaroh, Puspa Wardani, A. Aryati, Evy Diah Woelansari

{"title":"Validation of Rapid Antibody (IgM – IgG) Test Kit for SARS-CoV-2 Infection in Surabaya, Indonesia","authors":"Museyaroh Museyaroh, Puspa Wardani, A. Aryati, Evy Diah Woelansari","doi":"10.33086/ijmlst.v4i2.3027","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.3027","url":null,"abstract":"At the beginning of the Corona Virus Disease 2019 (COVID-19) pandemic, rapid test examinations were widely used as a screening for Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection. The purpose of this examination was to detect SARS-CoV-2 IgM/IgG antibodies in the patient's body. One of these tests uses the immunochromatographic method. This study aims to determine the validity of immunochromatography. The study was conducted from August to September 2020. The sample used in this study was 100 patients. The research was conducted at Husada Utama Hospital Surabaya, Indonesia. According to the study's findings, the Zybio brand reagent kit has an accuracy of 85%, a sensitivity of 82%, a specificity of 88%, a positive predictive value of 87%, and a negative predictive value of 83%. In the group of patients who experienced clinical symptoms, < 7 had a sensitivity of 50%, specificity of 88%, positive predictive value of 60%, negative predictive value of 83%, and accuracy of 77.94% while the group of patients experiencing clinical symptoms > 7 days, had a sensitivity value of 100 %, specificity of 88%, positive predictive value of 84%, negative predictive value of 100%, and accuracy of 92.68%. Based on these results, the conclusion is that the Zybio brand reagent kit has a relatively high sensitivity, specificity, positive predictive value, negative value, and sample accuracy. In the group with clinical sensitivity < 7 days, the positive predictive value and accuracy are lower than the sample group with clinical symptoms > 7 days but have the same specificity.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133338369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil Lymphocyte Ratio (NLR) in COVID-19 Patients Receiving Convalescent Plasma Therapy 新冠肺炎恢复期血浆治疗患者中性粒细胞淋巴细胞比率(NLR)的变化

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.2460

Desyani Ariza, Andi Maya Kesrianti, Tazya Anggraini Ruslan

{"title":"Neutrophil Lymphocyte Ratio (NLR) in COVID-19 Patients Receiving Convalescent Plasma Therapy","authors":"Desyani Ariza, Andi Maya Kesrianti, Tazya Anggraini Ruslan","doi":"10.33086/ijmlst.v4i2.2460","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.2460","url":null,"abstract":"Recently, a new RNA virus from the Coronaviridae family was discovered, known as SARS-Cov-2. This virus causes pneumonia and inflammation in the body. One of the laboratory tests used to see inflammation in the body is the Neutrophil Lymphocyte Ratio, or often abbreviated as NLR. NLR is one of the markers of inflammation that can be used simply, efficiently, and reliably because of its high stability and sensitivity. Higher NLR values tend to lead to a severe and poor prognosis, so this test can be done by monitoring patients with confirmed Corona Virus Disease 2019 (COVID-19). The purpose of this study was to determine the neutrophil lymphocyte’s ratio in COVID-19 patients receiving convalescent plasma therapy. This research method is a quantitative observation using a descriptive approach. Quantitative observational research is used to analyze data in the form of numbers from the results of laboratory tests. The results obtained from 17 research subjects, namely the NLR values before convalescent plasma therapy were obtained in as many as 6 patients (35%) with normal NLR values below 3.13 and NLR values are increasing above 3.13 in as many as 11 patients (65%). Meanwhile, after convalescent plasma therapy, there were 8 patients (47%) with normal NLR below 3.13 and NLR values increasing above 3.13 as many as 9 patients (53%). This research concerns about changes in NLR values before and after convalescent plasma therapy, which before convalescent plasma therapy there were 6 patients with normal NLR values and after convalescent plasma therapy increased to 8 patients with normal NLR values which indicate a better good prognosis.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"5 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123663244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological Profile of Therapeutic Doses of Methanolic Extract Combination of Tea and Mango Mistletoes in Cardiac of Preventive Hypertensive Rats (DOCA-salt) Model 茶与芒果槲皮醇提物联合用药剂量对预防高血压大鼠(DOCA-salt)模型心脏组织病理学的影响

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.2693

Durrotul Hasanah, Nour Athiroh Abdoes Sjakoer, N. Mubarakati

{"title":"Histopathological Profile of Therapeutic Doses of Methanolic Extract Combination of Tea and Mango Mistletoes in Cardiac of Preventive Hypertensive Rats (DOCA-salt) Model","authors":"Durrotul Hasanah, Nour Athiroh Abdoes Sjakoer, N. Mubarakati","doi":"10.33086/ijmlst.v4i2.2693","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.2693","url":null,"abstract":"Tea and mango mistletoes have to lower blood pressure by improving oxidative stress and endothelial dysfunction. The flavonoid compounds found in these two mistletoes act as antihypertensives. Hypertension is a risk factors that influences the incidence of cardiac disease. This study examines the histopathological of the cardiac of hypertensive rats (preventive model) after treatment of methanolic extract combination of tea and mango mistletoes. This study used a completely randomized experimental design with 25 Wistar rats in total for five treatments, namely negative control (-), positive control (+), treatment 1 (dose of 50 mg/kg BW), treatment 2 (doses of 100 mg/kg BW), and treatment 3 (doses of 200 mg/kg BW). In this study, Deoxycorticosterone acetate (DOCA) were used to induce hyperttension in experimental animals. Data were analysed with one-way ANOVA test using JAMOVI (version 1.1.9.0) and posteriori. There was a significant difference between all groups (p<0.05). Administration of a methanolic extract combination of tea and mango mistletoes as the preventive model can significantly reduce hypertrophy of cardiac muscle cells. This effect occured with a dose of 50 mg/kg BW, the optimal dose to reduce hypertrophy of cardiac muscle cells in Wistar rats male.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131810704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship of Albumin Concentrations with Sodium, Potassium, in CAPD (Continuous Ambulatory Peritoneal Dialysis) Patients at PHC Hospital Surabaya 泗水PHC医院持续动态腹膜透析患者白蛋白浓度与钠、钾的关系

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-10-30 DOI: 10.33086/ijmlst.v4i2.3252

D. Anggraini, E. Haryanto, S. Arifin, Ayu Puspitasari

{"title":"Relationship of Albumin Concentrations with Sodium, Potassium, in CAPD (Continuous Ambulatory Peritoneal Dialysis) Patients at PHC Hospital Surabaya","authors":"D. Anggraini, E. Haryanto, S. Arifin, Ayu Puspitasari","doi":"10.33086/ijmlst.v4i2.3252","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i2.3252","url":null,"abstract":"Kidney failure is caused by a permanent decline in kidney function and requires renal replacement therapy, one of which is Continuous Ambulatory Peritoneal Dialysis (CAPD). This study aimed to determine the relationship between albumin concentrations and sodium and potassium concentrations in CAPD patients. The study was carried out between January to May 2022. Cross-sectional research methodology is used in this study. It included 60 chronic kidney failure patients who had CAPD as therapy at PHC Surabaya Hospital. Then, patients were divided into three groups with an age interval of 40-50 years, 51-60 years, and 61-70 years in. The results of grouping CAPD patients according to the characteristics based on the age of CAPD patients aged 61-70 years had low albumin concentrations and low sodium concentrations A total of 12 people (66.67%), patients with large CAPD aged 51-60 years, had low albumin and potassium concentrations, namely 11 people (61.11%). CAPD patients aged 51-60 years mostly had low albumin and sodium concentrations in 13 people (72.22%). the conclusion is that in patients with renal failure on CAPD therapy, there is a decrease in albumin and sodium-potassium concentrations due to peritonitis (inflammation) which is associated with a measurable excess of fluid (overhydration) which causes more dilute sodium, causing hyponatremia and hypokalemia.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134019693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermatozoa Morphology Examination Using Lenshooke SQA X1 Pro Compared with Manual Method 用Lenshooke sqax1 Pro进行精子形态学检查与手工法比较

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-04-28 DOI: 10.33086/ijmlst.v4i1.2059

Adhipireno Purwanto, E. Purwanto, Suyono Seso Sulijaya, I. Emma, S. Budi, Limijadi Edward Kurnia Setiawan

{"title":"Spermatozoa Morphology Examination Using Lenshooke SQA X1 Pro Compared with Manual Method","authors":"Adhipireno Purwanto, E. Purwanto, Suyono Seso Sulijaya, I. Emma, S. Budi, Limijadi Edward Kurnia Setiawan","doi":"10.33086/ijmlst.v4i1.2059","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2059","url":null,"abstract":"According to World Health Organization data, 30-40% of infertility is caused by male factors. The morphology of normal spermatozoa is an indicator of male fertility, and it is known by manual or automatic sperm analysis. Lenshooke SQA X1 PRO automatic equipment comes along with the development of laboratory equipment automation technology. The working principle of this tool is by shining light on the object of examination, then the camera with high resolution, with the facility of an optical lens will take a picture of the object. The database recorded by the camera is analyzed by the algorithm. The research objective was to test the suitability of the Lenshooke SQA X1 PRO automatic tool with manual method as the Gold Standard. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA \"Restu Ibu\" Sragen from June to August 2020. The examination method used an automatic method with the Lenshooke SQA X1 PRO tool and a manual method with Papanicolaou staining. The results of the study, conformity test with WHO 2010 normal standards, automatic methods reached 94.4% compared to manual methods. The next statistical test was with standard mean, normal sperm morphology data had a significance of 0.001, abnormal sperm head data had a significance value of 0.956 and abnormal sperm tail data had a significance value of 0.339. The Lenshooke SQA XI PRO device based on automatic technology can be used in laboratory services for sperm analysis in addition to manual methods. Suggestions for using the Lenshooke XI PRO automatic tools are still accompanied by the manual method.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114996495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Collagen-VI Specific Primer Design Identification in Rats (Rattus norvegicus) Pancreas 褐家鼠胰腺胶原- vi特异性引物设计鉴定

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-04-28 DOI: 10.33086/ijmlst.v4i1.2515

E. Stujanna, Sri Suci Ningsih, Rizkyana Avissa, Novi Putri Ayu, Zahra Nurussofa, Dewi Jantika Djuarna, Rini Latifah, W. Sukarya

{"title":"Collagen-VI Specific Primer Design Identification in Rats (Rattus norvegicus) Pancreas","authors":"E. Stujanna, Sri Suci Ningsih, Rizkyana Avissa, Novi Putri Ayu, Zahra Nurussofa, Dewi Jantika Djuarna, Rini Latifah, W. Sukarya","doi":"10.33086/ijmlst.v4i1.2515","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2515","url":null,"abstract":"Type 2 diabetes mellitus, the most common diabetes type characterized by hyperglycemia, is caused by abnormal secretion and activity of pancreatic insulin enzymes. The extracellular matrix (ECM) plays a vital role in keeping β pancreatic cells intact and undissociated. The ECM in the pancreas can play a role in influencing insulin function and production. The most abundant ECM in the pancreas is collagen type VI. Collagen type VI has an essential role in the survival of pancreatic islet cells, including pancreatic β cells. Nowadays, Polymerase Chain Reaction (PCR) technology is widely utilized for molecular biology analysis. One of the most critical factors for successful PCR is designing the correct specific PCR primers. The objective of this study was to design a specific primer for collagen VI in the pancreas of Rattus norvegicus. The primer was designed and analyzed using MEGA.11, primer three-plus, and primer-BLAST. Five primer pairs were analyzed based on the characteristics of primer length, product amplicon length, Tm value, GC percentage, and secondary structure. Primer pair 3 (F:5’-TGTTTGGCTTTGTCGCGGGC-3’ and R:5’-TTGTTGCTGCCGACACTGGC-3’); Col6a2 (F:5’-TGTGGTCAACAGGCTGGGCG-3’ and R:5’-TCTGGCGCCGGCTCTCTTTG-3’) were considered as the best primer for the Collagen VI expression detection from the pancreas of Rattus norvegicus, which produce amplicon about 250pb and 245pb, respectively.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126777767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia MiR-141-3p在印尼日惹市FFPE样品中的相对表达水平作为前列腺癌发生的生物标志物

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-04-28 DOI: 10.33086/ijmlst.v4i1.2355

Sari Eka Pratiwi, S. N. Wahyuningrum, Rachmagreta Perdana Putri, Danarto Danarto, D. S. Heriyanto, N. Arfian, S. Haryana, I. Astuti

{"title":"MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia","authors":"Sari Eka Pratiwi, S. N. Wahyuningrum, Rachmagreta Perdana Putri, Danarto Danarto, D. S. Heriyanto, N. Arfian, S. Haryana, I. Astuti","doi":"10.33086/ijmlst.v4i1.2355","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2355","url":null,"abstract":"Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127871841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Correlation Results of Examination of Hemoglobin and The Erythrocyte Index in Patients With Suspected Covid-19 in The Hospital of Kendari City 肯达里市医院疑似新冠肺炎患者血红蛋白与红细胞指数相关性分析

Indonesian Journal of Medical Laboratory Science and Technology Pub Date : 2022-04-28 DOI: 10.33086/ijmlst.v4i1.2105

Sri Aprilianti Idris, Firdayanti Firdayanti, S. Susanti, Muh. Azdar Setiawan

{"title":"The Correlation Results of Examination of Hemoglobin and The Erythrocyte Index in Patients With Suspected Covid-19 in The Hospital of Kendari City","authors":"Sri Aprilianti Idris, Firdayanti Firdayanti, S. Susanti, Muh. Azdar Setiawan","doi":"10.33086/ijmlst.v4i1.2105","DOIUrl":"https://doi.org/10.33086/ijmlst.v4i1.2105","url":null,"abstract":"Coronavirus disease 2019 has become a global problem causing hundreds of thousands of deaths worldwide. Haemoglobin is more susceptible to COVID-19 virus attacks. Haemoglobin functions as a carrier of oxygen to organs in the body. When the concentration of haemoglobin in the blood circulation is low, the transport of oxygen to several organs in the body can be disrupted. SARS-CoV-2 interacts with haemoglobin in red blood cells. This interaction causes the virus to break the haemoglobin chain and cause hemolysis. The erythrocyte index which consists of MCV, MCH, and MCHC values is used to indicate the occurrence of anemia in COVID-19 patients. This research method used descriptive analytic with a cross sectional study design. The population of this study was suspected COVID-19 patients with a sample of 27 people. Sampling was carried out using purposive sampling. This study aims to determine the relationship between haemoglobin examination results and erythrocyte index in suspected COVID-19 patients.The instrument in this study used secondary data which included the results of examination of haemoglobin values and erythrocyte index. The results of the test using a parametric statistical approach with the correlation analysis method showed that there was a correlation between the results of the haemoglobin and MCV test (P-value 0.057 > 0.05), while the haemoglobin and MCH test had no correlation (P value 0.777 > 0.05), and there is no correlation between haemoglobin and MCHC examination (P-value 0.372 > 0.05).","PeriodicalId":158539,"journal":{"name":"Indonesian Journal of Medical Laboratory Science and Technology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130666814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0