Journal of General and Applied Microbiology最新文献_第2页

Impact of salinity and time on structure and functional potential of wastewater treatment biofilms in intermittent sand bioreactors. 盐度和时间对间歇式沙生物反应器中污水处理生物膜的结构和功能潜力的影响。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-07-20 Epub Date: 2024-01-18 DOI: 10.2323/jgam.2023.12.003

Kristen Conroy, Jelmer Poelstra, Karen Mancl

{"title":"Impact of salinity and time on structure and functional potential of wastewater treatment biofilms in intermittent sand bioreactors.","authors":"Kristen Conroy, Jelmer Poelstra, Karen Mancl","doi":"10.2323/jgam.2023.12.003","DOIUrl":"10.2323/jgam.2023.12.003","url":null,"abstract":"High salt wastewater is produced in industries, including seafood and pickling processing. The salinity in such wastewaters has been shown to negatively impact biological treatment efficacy. Little is known about the changes in the microbial community structure in the mature biological 2 treatment systems, the impacts of salinity on community composition, and the shifts over time during operation. This study aimed to identify the changes in the microbial community due to both salt and days of operation through 16s rRNA sequencing and KEGG functional predictions. Intermittent sand bioreactors (ISBs) with a focus on ammonia treatment were utilized. Results showed that the overall community structure and diversity were distinct as wastewater salinity varied from 0%-1.3%. At 1.3% salinity Zoogloea, a common genus in wastewater treatment plants, was not present and Aequorovita, Thauera and Dokdonella became the dominant genera. Nitrosomonas, an important ammonia oxidizing bacteria, increased in abundance with days of operation but was not significantly impacted by an increase in salinity. This finding was further supported by an increase in predicted nitrification potential with time of operation within all intermittent sand bioreactors tested. These results provide a deeper understanding of the impacts of salinity on microbial community development in biological treatment systems and elucidate the shifts in community structure occurring during early operations and into system maturity.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic promoter design for plasmid-encoded S-adenosylmethionine sensing systems. 质粒编码的 S-腺苷蛋氨酸传感系统的系统性启动子设计。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-07-20 Epub Date: 2024-01-29 DOI: 10.2323/jgam.2024.01.002

Taro Watanabe, Yuki Kimura, Daisuke Umeno

{"title":"Systematic promoter design for plasmid-encoded S-adenosylmethionine sensing systems.","authors":"Taro Watanabe, Yuki Kimura, Daisuke Umeno","doi":"10.2323/jgam.2024.01.002","DOIUrl":"10.2323/jgam.2024.01.002","url":null,"abstract":"S-adenosylmethionine (SAM) is an important biomolecule that mainly acts as a methyl donor and plays many roles in a variety of biological functions. SAM is also required for the biosynthesis of valuable methylated compounds, but its supply is a bottleneck for these biosynthetic pathways. To overcome this bottleneck and to reconfigure SAM homeostasis, a high-throughput sensing system for changes in intracellular SAM availability is required. We constructed a plasmid that can detect the factors that can alter SAM availability using minimal components. It does so by placing a fluorescent protein under a promoter controlled by endogenous MetJ, a transcription factor that represses its own regulons upon binding with SAM. Next, to validate SAM-responsive behavior, we systematically reconstructed 10 synthetic promoters with different positions and with different number of metbox sites. We found that a position between the -35 box and the -10 box was the most effective for repression and that this setup was suitable for detecting the genetic or environmental factors that can deplete and recover the intracellular SAM availability. Overall, the response patterns of the synthetic MetJ-regulated promoters characterized in this study may be useful for the development of better SAM biosensing systems.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139570013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of the Csr global regulatory system mediated by small RNA decay in Aeromonas salmonicida. 鉴定沙门氏菌中由小 RNA 降解介导的 Csr 全局调控系统。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-07-20 Epub Date: 2024-01-18 DOI: 10.2323/jgam.2023.12.004

Olga Gladyshchuk, Masaki Yoshida, Koume Togashi, Hayuki Sugimoto, Kazushi Suzuki

{"title":"Identification of the Csr global regulatory system mediated by small RNA decay in Aeromonas salmonicida.","authors":"Olga Gladyshchuk, Masaki Yoshida, Koume Togashi, Hayuki Sugimoto, Kazushi Suzuki","doi":"10.2323/jgam.2023.12.004","DOIUrl":"10.2323/jgam.2023.12.004","url":null,"abstract":"We investigated the presence and functionality of the carbon storage regulator (Csr) system in Aeromonas salmonicida SWSY-1.411. CsrA, an RNA-binding protein, shared 89% amino acid sequence identity with Escherichia coli CsrA. CsrB/C sRNAs exhibited a typical stem-loop structure, with more GGA motifs, which bind CsrA, than E. coli. CsrD had limited sequence identity with E. coli CsrD; however, it contained the conserved GGDEF and EAL domains. Functional analysis in E. coli demonstrated that the Csr system of A. salmonicida influences glycogen biosynthesis, biofilm formation, motility, and stability of both CsrB and CsrC sRNAs. These findings suggest that in A. salmonicida, the Csr system affects phenotypes like its E. coli counterpart. In A. salmonicida, defects in csr homologs affected biofilm formation, motility, and chitinase production. However, glycogen accumulation and protease production were unaffected. The expression of flagellar-related genes and chitinase genes was suppressed in the csrA-deficient A. salmonicida. Northern blot analysis indicated the stabilization of CsrB and CsrC in the csrD-deficient A. salmonicida. Similar to that in E. coli, the Csr system in A. salmonicida comprises the RNA-binding protein CsrA, the sRNAs CsrB and CsrC, and the sRNA decay factor CsrD. This study underscores the conservation and functionality of the Csr system and raises questions about its regulatory targets and mechanisms in A. salmonicida.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of phthalic acid esters (PAEs) by Janthinobacterium sp. strain E1 under stress conditions. Janthinobacterium sp. 菌株 E1 在压力条件下对邻苯二甲酸酯 (PAE) 的生物降解。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-07-20 Epub Date: 2024-01-15 DOI: 10.2323/jgam.2023.12.002

Kailu Zhang, Hui Zhou, Juntao Ke, Hongli Feng, Cunlong Lu, Shaoxing Chen, Aimin Liu

{"title":"Biodegradation of phthalic acid esters (PAEs) by Janthinobacterium sp. strain E1 under stress conditions.","authors":"Kailu Zhang, Hui Zhou, Juntao Ke, Hongli Feng, Cunlong Lu, Shaoxing Chen, Aimin Liu","doi":"10.2323/jgam.2023.12.002","DOIUrl":"10.2323/jgam.2023.12.002","url":null,"abstract":"Phthalates esters (PAEs) are a kind of polymeric material additives widely been added into plastics to improve products' flexibility. It can easily cause environmental pollution which are hazards to public health. In this study, we isolated an efficient PAEs degrading strain, Janthinobacterium sp. E1, and determined its degradation effect of di-2-ethylhexyl phthalate (DEHP) under stress conditions. Strain E1 showed an obvious advantage in pollutants degradation under various environmental stress conditions. Degradation halo clearly occurred around the colony of strain E1 on agar plate supplemented with triglyceride. Strain E1's esterase is a constitutively expressed intracellular enzyme. The esterase purified from strain E1 showed a higher catalytic effect on short-chain PAEs than long-chain PAEs. The input of DEHP, DBP (dibutyl phthalate) and DMP (dimethyl phthalate) into the tested soil did not change the species composition of soil prokaryotic community, but altered the dominant species in specific environmental conditions. And the community diversity and richness decreased to a certain extent. However, the diversity and richness of the microbial community were improved after the contaminated soil was treated with the strain E1. Our results also suggested that strain E1 exhibited a tremendous potential in environmental bioremediation in the real environment, which provides a new insight into the elimination of the pollutants contamination in the urban environment.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Zn solubilizing bacteria (ZSB) mitigate toxicity of silver and Titanium dioxide nanoparticles in Mung bean by increasing photosynthetic pigment content. 锌溶解菌（ZSB）通过增加光合色素含量来减轻纳米银和二氧化钛对绿豆的毒性。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-06-27 DOI: 10.2323/jgam.2024.05.005

Mahvash Haroon, Shams Tabrez Khan, Abdul Malik

{"title":"Zn solubilizing bacteria (ZSB) mitigate toxicity of silver and Titanium dioxide nanoparticles in Mung bean by increasing photosynthetic pigment content.","authors":"Mahvash Haroon, Shams Tabrez Khan, Abdul Malik","doi":"10.2323/jgam.2024.05.005","DOIUrl":"https://doi.org/10.2323/jgam.2024.05.005","url":null,"abstract":"Zn-deficiency, a global health challenge affects one-third of the world population. Zn-biofertilizer offer an efficient and cost-effective remedy. As Zn-biofertilizer can improve plant growth and grain's Zn-content ensuring improved dietary Zn-supply. This study sought to understand how silver and TiO2 nanoparticles in the rhizosphere affect the activity of Zn-solubilization bacteria (ZSB) and plant growth. Two ZSB strains Bacillus sp. D-7 and Pseudomonas sp. D-117 with excellent Zn-solubilization efficiency of 254 and 260%, respectively were isolated and characterized using polyphasic characterization including 16S rRNA gene sequencing to formulate an effective Zn-biofertilizer. The plant growth promoting activity of this biofertilizer in Mung bean was checked in the presence and absence of various doses of TiO2 and Ag-NPs and was compared with plant grown without biofertilizer. The change in rate of seed germination, vegetative growth (shoot and root length, fresh and dry weight), photosynthetic pigment and Zn-content was checked. Lower doses of nanomaterials (50 and 100 mg kg⁻¹ soil) slightly promoted the plant growth compared to control. While, higher doses (200 and 400 mg kg⁻¹ soil) inhibited the growth. A maximum decrease of shoot length, root length, fresh-weight, and dry-weight of 57.1, 53.9, 53.1, and 10.4% respectively was observed with 400 mg kg⁻¹ of Ag-NPs. However, in the presence of ZSB, the decrease at the same Ag-NP concentration was 41.6, 31.5, 27.4, and 6.6, respectively. These results strongly suggest that Zn-solubilizing bacteria improve resilience to nanoparticles toxicity and helps in Zn fortification in Mung bean even under nanomaterial stress.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rational Design of a Yeast-derived 3',5'-bisphosphate Nucleotidase with Improved Substrate Specificity. 合理设计源于酵母的 3',5'-二磷酸核苷酸酶，提高其底物特异性。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-06-18 DOI: 10.2323/jgam.2024.05.006

Jipeng Jiang, Yanqing Sun, Yanan Sun, Fuping Lu, Fufeng Liu, Huitu Zhang

{"title":"Rational Design of a Yeast-derived 3',5'-bisphosphate Nucleotidase with Improved Substrate Specificity.","authors":"Jipeng Jiang, Yanqing Sun, Yanan Sun, Fuping Lu, Fufeng Liu, Huitu Zhang","doi":"10.2323/jgam.2024.05.006","DOIUrl":"https://doi.org/10.2323/jgam.2024.05.006","url":null,"abstract":"In recent years, a convenient phosphatase-coupled sulfotransferase assay method has been proven to be applicable to most sulfotransferases. The central principle of the method is that phosphatase specifically degrades 3'-phosphoadenosine-5'-phosphate (PAP) and leaves 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Our group previously acquired a yeast 3',5'-bisphosphate nucleotidase (YND), which showed a higher catalytic activity for PAP than PAPS and could be a potential phosphatase for the sulfotransferase assay. Here, we obtained a beneficial mutant of YND with markedly improved substrate specificity towards PAP via rational design. Of 9 chosen mutation sites in the active site pocket, the mutation G236D showed the best specificity for PAP. After optimization of the reaction conditions, the mutant YNDG236D displayed a 4.8-fold increase in the catalytic ratio PAP/PAPS compared to the wild-type. We subsequently applied YNDG236D to the assay of human SULT1A1 and SULT1A3 with their known substrate 1-naphthol, indicating that the mutant could be used to evaluate sulfotransferase activity by colorimetry. Analysis of the MD simulation results revealed that the improved substrate specificity of the mutant towards PAP may stem from a more stable protein conformation and the changed flexibility of key residues in the entrance of the substrate tunnel. This research will provide a valuable reference for the development of efficient sulfotransferase activity assays.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141427003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Directed evolution of highly sensitive and stringent choline-induced gene expression controllers. 高灵敏度和严格的胆碱诱导基因表达控制器的定向进化。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-06-17 DOI: 10.2323/jgam.2024.05.004

Yuki Yanai, Takayuki Hoshino, Yuki Kimura, Shigeko Kawai-Noma, Daisuke Umeno

{"title":"Directed evolution of highly sensitive and stringent choline-induced gene expression controllers.","authors":"Yuki Yanai, Takayuki Hoshino, Yuki Kimura, Shigeko Kawai-Noma, Daisuke Umeno","doi":"10.2323/jgam.2024.05.004","DOIUrl":"https://doi.org/10.2323/jgam.2024.05.004","url":null,"abstract":"Gene expression controllers are useful tools for microbial production of recombinant proteins and valued bio-based chemicals. Despite its usefulness, they have rarely been applied to the practical industrial bioprocess, due to the lack of systems that meets the three requirements: low cost, safety, and tight control, to the inducer molecules. Previously, we have developed the high-spec gene induction system controlled by safe and cheap inducer choline. However, the system requires relatively high concentration (~100 mM) of choline to fully induce the gene under control. In this work, we attempted to drastically improve the sensitivity of this induction system to further reduce the induction costs. To this end, we devised a simple circuit which couple gene induction system with positive-feedback loop (P-loop) of choline importer protein BetT. After the tuning of translation level of BetT (strength of the P-loop) and deletion of endogenous betI (noise sources), highly active yet stringent control of gene expression was achieved using about 100 times less amount of inducer molecules. The choline induction system developed in this study has the lowest basal expression, the lowest choline needed to be activated, and the highest amplitude of induction as the highest available promoter such as those known as PT5 system. With this system, one can tightly control the expression level of genes of interest with negligible cost for inducer molecule, which has been the bottleneck for the application to the large-scale industrial processes.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The chromosome level whole genome sequence and the seconary matabolism gene cluster prediction of Fusarium meridionale, the pathogen causing maize ear rot. 玉米穗腐病病原 Fusarium meridionale 的染色体水平全基因组序列和secondary matabolism 基因簇预测。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-05-28 DOI: 10.2323/jgam.2024.05.003

Qing Du, Zhaolei Tang, Juegui Su, Shichu Li

{"title":"The chromosome level whole genome sequence and the seconary matabolism gene cluster prediction of Fusarium meridionale, the pathogen causing maize ear rot.","authors":"Qing Du, Zhaolei Tang, Juegui Su, Shichu Li","doi":"10.2323/jgam.2024.05.003","DOIUrl":"https://doi.org/10.2323/jgam.2024.05.003","url":null,"abstract":"Fusarium meridionale is one of the pathogens causing maize ear rot, it produce bioactive secondary metabolites may threaten humans food safty, however, the production mechanism of the secondary metabolites and their interaction with maize ear remains poorly understood. To facilitate related studies, we sequenced and assembled the genome of F. meridionale strain JX18-4. The size of F. meridionale JX18-4 genome is 37.11 Mbp, include four nuclear chromosome contigs that consists of 11920 coding genes and one mitochondrial contig. 95.64% gene synteny collinearity was found between the assembly and the reference genomes F. graminearum strain PH-1. Compared to the sequences of seconary matabolism gene clusters sequences reported previously, the stain JX18-4 was predicted potential producing 8 clusters, including nivalenol, zearalenone, aurofusarin, fusarielin, fusaristatin A, fusarin, fusarubin and butenolide. This study aims to reveal the molecular mechanism of secondary metabolites producing, and the genomic information of JX18-4 will provide resources for the study of biological control mechanisms and plant-microbe interactions.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141175142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure of the SigF1-dependent pilA1 gene promoter and characterization of the light-activated response in the cyanobacterium Synechococcus elongatus PCC 7942. SigF1 依赖性 pilA1 基因启动子的结构和蓝藻细长球藻 PCC 7942 光激活反应的特征。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-05-20 DOI: 10.2323/jgam.2024.05.002

Ying Luo, Hitomi Imamitsu, Tatsuhiro Tsurumaki, Kan Tanaka

引用次数: 0

Marine bacteria have multiple polyamide 4-degrading enzymes. 海洋细菌具有多种聚酰胺 4 降解酶。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-05-13 DOI: 10.2323/jgam.2024.05.001

Yusuke Saito, Ibuki Jin, Miwa Yamada

{"title":"Marine bacteria have multiple polyamide 4-degrading enzymes.","authors":"Yusuke Saito, Ibuki Jin, Miwa Yamada","doi":"10.2323/jgam.2024.05.001","DOIUrl":"https://doi.org/10.2323/jgam.2024.05.001","url":null,"abstract":"Polyamide 4 (PA4) is expected to solve the issue of marine plastic pollution due to its excellent mechanical properties and biodegradability. In this study, to reveal the mechanism of PA4 biodegradation in the marine environment, we isolated 5 strains of PA4-degrading bacteria belonging to Aliiglaciecola, Dasania, and Pseudophaeobacter from a marine environment. The isolated 5 strains are novel PA4-degrading bacteria that are phylogenetically distinct from those isolated in previous studies. In addition, we compared the PA4-degrading activities and structures of the PA4-degrading enzymes secreted by the 5 strains and PA4-degrading strains isolated in our previous study. The PA4-degrading activity in the supernatant of the cultivation solutions differed among the strains. Native-PAGE and zymography using a polyacrylamide gel containing a PA4 emulsion demonstrated that PA4-degrading enzymes are classified into no less than three types of structures. These results suggested that marine PA4-degrading bacteria have multiple PA4-degrading enzymes. Our findings will contribute to a better understanding of the microbial degradation of PA4 in the marine environment.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0