Journal of General and Applied Microbiology最新文献_第2页

Bacillus subtilis var. natto as a naturally optimized biotin biosensor. 纳豆作为天然优化的生物素生物传感器。

IF 0.6 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-29 DOI: 10.2323/jgam.2026.06.004

Wakana Suda, Yoshiaki Ohashi, Nobuaki Kono

引用次数: 0

Biotin Dependency Shapes Sporulation Kinetics in Bacillus subtilis var. natto. 生物素依赖性影响纳豆枯草芽孢杆菌的产孢动力学。

IF 0.6 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-25 DOI: 10.2323/jgam.2026.06.003

Wakana Suda, Shojiro Kitajima, Kei Asai, Yoshiaki Ohashi, Nobuaki Kono

引用次数: 0

FAS2-1280F mutation in the fatty acid synthase gene, FAS2, of Saccharomyces cerevisiae enhances caprylic acid production. 酿酒酵母脂肪酸合成酶基因FAS2- 1280f突变可促进辛酸的生产。

IF 0.6 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-23 DOI: 10.2323/jgam.2026.06.002

Yuji Tasaki, Sakurako Sato, Yuki Taguchi, Ibuki Tada, Takashi Kuribayashi, Toshio Aoki

引用次数: 0

Reversible dissociation of peripheral subunits from the photosynthetic reaction center of the green sulfur bacterium Chlorobaculum tepidum. 绿硫细菌的外周亚基从光合反应中心可逆解离。

IF 0.6 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-19 DOI: 10.2323/jgam.2026.06.001

Tomomi Inagaki, Kazuki Terauchi, Chihiro Azai

{"title":"Reversible dissociation of peripheral subunits from the photosynthetic reaction center of the green sulfur bacterium Chlorobaculum tepidum.","authors":"Tomomi Inagaki, Kazuki Terauchi, Chihiro Azai","doi":"10.2323/jgam.2026.06.001","DOIUrl":"https://doi.org/10.2323/jgam.2026.06.001","url":null,"abstract":"The photosynthetic reaction center (RC) of green sulfur bacteria is divided into two parts: the membrane-embedded RC core (RCC) complex (comprising PscA and PscC) and the water-soluble peripheral subunits (comprising the trimeric Fenna-Matthews-Olson [FMO] protein, PscB, and PscD). The peripheral subunits are bound onto the cytoplasmic surface of the RCC complex, and no method has been identified so far to detach the peripheral subunits to maintain the functionality of the RCC complex. The present study proposes biochemical treatments enabling the reversible dissociation of the peripheral subunits. A pH increased to 11.0 effectively removed almost all the peripheral subunits from the RCC complex. NaCl of more than 1 M destabilized their binding; however, the degrees of impact differed between each subunit. Approximately half of FMO and PscB remained on the RCC complex, whereas PscD demonstrated near-complete dissociation. Continuous washing of the detached subunits led to the near-complete dissociation of all subunits, even when treated with 1 M NaCl. The peripheral subunits under the detaching conditions were in an equilibrated state between the bound and unbound states. Neutralization and desalting after detaching treatments reassembled all the dissociated subunits and recovered the charge-separation activity comparable to that before treatment. Insights from atomic resolution structures indicate that electrostatic interaction with PscA stabilized the binding of the peripheral subunits on the RCC complex.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148295292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Betaine and choline are important compatible solutes in Bacillus altitudinis under salt and glucose stress. 甜菜碱和胆碱是高海拔芽孢杆菌在盐和葡萄糖胁迫下重要的相容溶质。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-08 DOI: 10.2323/jgam.2026.05.004

Triwibowo Yuwono, Hazana Insyi'ta Sahla, M Saifur Rohman, Panjisakti Basunanda

{"title":"Betaine and choline are important compatible solutes in Bacillus altitudinis under salt and glucose stress.","authors":"Triwibowo Yuwono, Hazana Insyi'ta Sahla, M Saifur Rohman, Panjisakti Basunanda","doi":"10.2323/jgam.2026.05.004","DOIUrl":"https://doi.org/10.2323/jgam.2026.05.004","url":null,"abstract":"Osmotolerant rhizobacteria, Bacillus altitudinis TR-19, has been subjected to osmotic stress by cultivating the cells in: (1) Luria Bertani (LB) broth medium, (2) LB broth supplemented with 1.8M NaCl, and (3) LB broth supplemented with 20% glucose incubated at 370C with shaking. Samples were drawn periodically for growth analysis and metabolome profile. The results demonstrated that cultivation of the osmotolerant rhizobacteria in 1.8M NaCl-supplemented LB medium and 20% glucose-supplemented LB medium prolonged the adaptation growth phase, suggesting that NaCl at 1.8 M and 20% glucose reduced cell growth rate, although the cells were still capable of growing at such high osmotic stress. Metabolites synthesised under different growth conditions, in rich media (LB broth) and under osmotic stress (1.8M NaCl-supplemented LB medium and 20% glucose-supplemented LB medium) also demonstrated striking differences in their profile. Under osmotic conditions in NaCl, much less metabolites were found than under non-stress condition, suggesting the changes in metabolites synthesis pathway. Under 20% glucose stress conditions, by using 20% glucose-supplemented LB medium, the number of metabolites were also found reduced but more metabolites were observed than under NaCl stress. Different metabolome profile under non-stress conditions, NaCl-stress, and glucose stress were also observed, suggesting the changes in the metabolic pathway. One of the metabolite synthesised under osmotic condition was betaine which is known as the derivative of amino acid glycine. Betaine was synthesised under NaCl and glucose stress. Under non-stress condition, choline was not detected, suggesting that betaine and choline are important compatible solutes under NaCl stress.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural amylase expression enhances starch utilization in Limosilactobacillus fermentum PJG11 from Dioscorea luzonensis. 天然淀粉酶表达增强吕宋薯蓣发酵乳酸杆菌PJG11对淀粉的利用

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-03 Epub Date: 2026-03-11 DOI: 10.2323/jgam.2026.02.001

Suerte M Baranda, Jimmbeth Zenila P Fabia, Grace Sheila P Jalani, Arc Josam J Racho, Marvielyn P Olivar, Lemuel Ray T Balloyan, Ma Joy Theresa T Agcaoili, Peter James Icalia Gann

{"title":"Natural amylase expression enhances starch utilization in Limosilactobacillus fermentum PJG11 from Dioscorea luzonensis.","authors":"Suerte M Baranda, Jimmbeth Zenila P Fabia, Grace Sheila P Jalani, Arc Josam J Racho, Marvielyn P Olivar, Lemuel Ray T Balloyan, Ma Joy Theresa T Agcaoili, Peter James Icalia Gann","doi":"10.2323/jgam.2026.02.001","DOIUrl":"10.2323/jgam.2026.02.001","url":null,"abstract":"Lactic acid bacteria (LAB) are widely recognized for their health benefits and are commonly incorporated into functional foods. However, their survival and metabolic performance depend on the availability of appropriate substrates. This study evaluated the ability of LAB isolated from Dioscorea species to utilize starch from Dioscorea luzonensis, an endemic plant in the Philippines. Among the isolates tested, Limosilactobacillus fermentum PJG11 demonstrated the highest efficiency in converting D. luzonensis starch into glucose. This enhanced starch utilization can be explained by the upregulation of the α-amylase gene when the strain was cultured with its natural substrate, resulting in accelerated starch granule degradation. These findings underscore the importance of using natural, plant-derived substrates in the development of LAB-based functional foods to support optimal bacterial activity and improve carbohydrate breakdown for enhanced nutritional value.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing polysaccharide biosynthesis in Sanghuangporus baumii through phosphoglucose isomerase gene silencing. 磷酸葡萄糖异构酶基因沉默促进桑黄孢多糖生物合成。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-03 Epub Date: 2026-01-22 DOI: 10.2323/jgam.2026.1.002

Bin Yu, Jianzhong Yao, Xiaole Liu, Siyao Hu, Siyuan Chang, Yuan Zhang, Tianyi Chen, Tianpeng Chen, Wenjun Sun, Yong Chen, Binfeng Li

{"title":"Enhancing polysaccharide biosynthesis in Sanghuangporus baumii through phosphoglucose isomerase gene silencing.","authors":"Bin Yu, Jianzhong Yao, Xiaole Liu, Siyao Hu, Siyuan Chang, Yuan Zhang, Tianyi Chen, Tianpeng Chen, Wenjun Sun, Yong Chen, Binfeng Li","doi":"10.2323/jgam.2026.1.002","DOIUrl":"10.2323/jgam.2026.1.002","url":null,"abstract":"Sanghuangporus baumii polysaccharides (SBP) are recognized for their valuable pharmacological activities, driving increasing interest in their medicinal potential. However, the biosynthetic pathway of SBP remains incompletely characterized. Phosphoglucose isomerase (PGI), a key enzyme in carbohydrate metabolism, catalyzes the reversible isomerization between glucose-6-phosphate (G-6-P) and fructose-6-phosphate (F-6-P) and is hypothesized to regulate polysaccharide biosynthesis in this fungus. In this study, the pgi gene from S. baumii (sbpgi) was cloned and created sbpgi-silenced mutants using RNA interference (RNAi) to investigate its function. Silencing sbpgi resulted in an approximately 20% reduction in mycelial biomass but concurrently enhanced the production of exopolysaccharide (EPS) and intracellular polysaccharide (IPS) by approximately 2.0-fold and 1.9-fold after 9 days, respectively. Furthermore, suppression of sbpgi expression markedly decreased the content of cell wall β-1,3-glucan (by ~23%) while increasing chitin deposition by about 1.7-fold, leading to alterations in cell wall architecture, including thickness, and changes in stress tolerance. Transcriptional analysis revealed that sbpgi silencing significantly upregulated the expression of key genes in the polysaccharide biosynthetic pathway, including ugpg and pmm, highlighting the critical regulatory role of sbpgi in polysaccharide production. Our findings provide a foundation for metabolic engineering strategies to develop high-yielding strains for the industrial production of SBP.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AvaKa, a protein of unknown function, contributes to iron homeostasis in the cyanobacterium Anabaena sp. strain PCC 7120. AvaKa是一种功能未知的蛋白质，在蓝藻Anabaena sp.菌株PCC 7120中有助于铁稳态。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-03 Epub Date: 2026-01-17 DOI: 10.2323/jgam.2026.1.001

Nozomi Kikuchi, Kento Tominaga, Eno Taniguchi, Shigeki Ehira

引用次数: 0

Physiological and biochemical changes and microbial community succession during the postharvest rot process of Stropharia rugosoannulata. 褐环棘球藻采后腐烂过程中生理生化变化及微生物群落演替

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-03 Epub Date: 2026-03-13 DOI: 10.2323/jgam.2026.03.001

Zhenggang Han, Yawei Wang, Jiangke Yang

{"title":"Physiological and biochemical changes and microbial community succession during the postharvest rot process of Stropharia rugosoannulata.","authors":"Zhenggang Han, Yawei Wang, Jiangke Yang","doi":"10.2323/jgam.2026.03.001","DOIUrl":"10.2323/jgam.2026.03.001","url":null,"abstract":"This study systematically elucidated the microbial community succession and functional gene dynamics during the postharvest spoilage process of Stropharia rugosoannulata by integrating physiological and biochemical indicators with metagenomic analysis. The experimental results demonstrated that as storage time extended, the activities of antioxidant enzymes (superoxide dismutase, peroxidase) in S. rugosoannulata significantly declined, while the content of membrane lipid peroxidation product malondialdehyde increased, leading to compromised cell membrane integrity and creating favorable conditions for microbial colonization. Metagenomic analysis revealed that during the spoilage phase (post-harvest day 14), the relative abundance of Pseudomonadota increased to 85.7%, with Pseudomonas replacing Ewingella as the absolutely dominant microbial population. Further functional gene analysis showed that the post-harvest day 14 exhibited significant enrichment of glycosyltransferases (GT0, GT1, GT2, GT4) and carbohydrate-binding modules (CBM10, CBM16, CBM50), along with pectinase (GH78), chitinase (GH19), and polysaccharide-modifying enzymes (CE4, CE11). This indicated a metabolic shift towards cell wall synthesis and substrate recognition. In contrast, the post-harvest day 7, prior to fruiting body softening, demonstrated high expression of glycoside hydrolases (GH1, GH2, GH4, GH94) and carbohydrate esterase CE8, focusing on the degradation of cellulose and starch. These findings, for the first time from a molecular ecology perspective, clarify that the essence of postharvest spoilage in S. rugosoannulata is a quality deterioration process driven by a Pseudomonas-dominated microbial community. The study provided a basis for the development of targeted antibacterial preservation strategies.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tunable heterologous phosphatidylcholine biosynthetic system reveals distinct phosphatidylcholine requirements for strong and weak acid responses in Acetobacter pasteurianus. 一个可调节的异源磷脂酰胆碱生物合成系统揭示了巴氏醋酸杆菌对强酸和弱酸反应的不同磷脂酰胆碱需求。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2026-06-03 Epub Date: 2026-03-19 DOI: 10.2323/jgam.2026.03.002

Md Riad Hossain Khan, Yosuke Toyotake, Mamoru Wakayama

引用次数: 0