Journal of General and Applied Microbiology最新文献_第10页

Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis? 幽门螺杆菌感染是否影响慢性胃窦炎患者胃黏膜和胃液中的细菌结构?

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-05-29 DOI: 10.2323/jgam.2020.08.005

Yang Jiang, Fei Meng, Ying Liu, Liyun Zheng, Shufang Ye, Jianmei Zhang

{"title":"Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?","authors":"Yang Jiang, Fei Meng, Ying Liu, Liyun Zheng, Shufang Ye, Jianmei Zhang","doi":"10.2323/jgam.2020.08.005","DOIUrl":"https://doi.org/10.2323/jgam.2020.08.005","url":null,"abstract":"This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"179-185"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39047952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D. Vph1与D型新型隐球菌铜稳态有关。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-03 DOI: 10.2323/jgam.2021.02.001

Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara

{"title":"Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D.","authors":"Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara","doi":"10.2323/jgam.2021.02.001","DOIUrl":"https://doi.org/10.2323/jgam.2021.02.001","url":null,"abstract":"We clarified the roles of VPH1 in Cryptococcus neoformans serotype D by examining the detailed phenotypes of VPH1-deficient cells (Δvph1) in terms of their capability to grow in acidic and alkaline pH, at a high temperature, and under high osmotic conditions, in addition to the involvement of VPH1 in copper (Cu) homeostasis and the expression of some C. neoformans virulence factors. Δvph1 could grow well on minimal medium (YNB) but exhibited hypersensitivity to 20 μM Cu due to the failure to induce Cu-detoxifying metallothionein genes (CMT1 and CMT2). In contrast, Δvph1 exhibited defective growth on rich medium (YPD), and the induction of Cu transporter genes (CTR1 and CTR4) did not occur in this medium, implying that this strain was incapable of the uptake of Cu ions for growth. However, the addition of excess Cu promoted CTR gene expression and supported Δvph1 growth. These results suggested that the lack of the VPH1 gene disturbed Cu homeostasis in C. neoformans. Moreover, the loss of Vph1 function influenced the urease activity of C. neoformans.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"195-206"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequences of two strains of Lactococcus lactis subsp. cremoris with the same ancestry but a different capacity to produce exopolysaccharides. 两株乳酸乳球菌亚种的基因组序列分析。祖先相同但产生外多糖的能力不同的Cremoris。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-30 DOI: 10.2323/jgam.2021.03.001

Yayoi Gotoh, Kyosuke Kita, Kosei Tanaka, Shu Ishikawa, Toshio Suzuki, Ken-Ichi Yoshida

引用次数: 1

Identification, characterization, and cloning of a novel aminoacylase, L-pipecolic acid acylase from Pseudomonas species. 假单胞菌中l -胡椒酸酰化酶的鉴定、鉴定和克隆。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-06-26 DOI: 10.2323/jgam.2020.12.001

Junji Hayashi, Yoshiaki Ichiki, Akiko Kanda, Kazuyoshi Takagi, Mamoru Wakayama

{"title":"Identification, characterization, and cloning of a novel aminoacylase, L-pipecolic acid acylase from Pseudomonas species.","authors":"Junji Hayashi, Yoshiaki Ichiki, Akiko Kanda, Kazuyoshi Takagi, Mamoru Wakayama","doi":"10.2323/jgam.2020.12.001","DOIUrl":"https://doi.org/10.2323/jgam.2020.12.001","url":null,"abstract":"L-Pipecolic acid is utilized as a vital component of specific chemical compounds, such as immunosuppressive drugs, anticancer reagents, and anesthetic reagents. We isolated and characterized a novel L-aminoacylase, N-acetyl-L-pipecolic acid-specific aminoacylase (LpipACY), from Pseudomonas sp. AK2. The subunit molecular mass of LpipACY was 45 kDa and was assumed to be a homooctamer in solution. The enzyme exhibited high substrate specificity toward N-acetyl-L-pipecolic acid and a high activity for N-acetyl-L-pipecolic acid and N-acetyl-L-proline. This enzyme was stable at a high temperature (60°C for 10 min) and under an alkaline pH (6.0-11.5). The N-terminal and internal amino acid sequences of the purified enzyme were STTANTLILRNG and IMASGGV, respectively. These sequences are highly consistent with those of uncharacterized proteins from Pseudomonas species, such as amidohydrolase and peptidase. We also cloned and overexpressed the gene coding LpipACY in Escherichia coli. Moreover, the recombinant LpipACY exhibited properties similar to native enzyme. Our results suggest that LpipACY is a potential enzyme for the enzymatic synthesis of L-pipecolic acid. This study provides the first description of the enzymatic characterization of L-pipecolic acid specific amino acid acylase.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"186-194"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39111741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide. 蓝藻双球菌FPU101合成新分离多糖的生理和基因组分析及外多糖的化学分析。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-10 DOI: 10.2323/jgam.2021.02.002

Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki

{"title":"Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide.","authors":"Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki","doi":"10.2323/jgam.2021.02.002","DOIUrl":"https://doi.org/10.2323/jgam.2021.02.002","url":null,"abstract":"A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated. The isolate, named Chroococcus sp. FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1. Purified EPS from Chroococcus sp. FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7. The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM. However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS. The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp. FPU101, which was sequenced in this study. These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"207-213"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Corynebacterium glutamicum Mycoredoxin 3 protects against multiple oxidative stresses and displays thioredoxin-like activity. 谷氨酰胺棒状杆菌核氧化还蛋白3可以抵抗多种氧化应激并显示类似硫氧还蛋白的活性。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-10-22 Epub Date: 2020-10-30 DOI: 10.2323/jgam.2019.10.003

Tao Su, Chengchuan Che, Ping Sun, Xiaona Li, Zhijin Gong, Jinfeng Liu, Ge Yang

{"title":"Corynebacterium glutamicum Mycoredoxin 3 protects against multiple oxidative stresses and displays thioredoxin-like activity.","authors":"Tao Su, Chengchuan Che, Ping Sun, Xiaona Li, Zhijin Gong, Jinfeng Liu, Ge Yang","doi":"10.2323/jgam.2019.10.003","DOIUrl":"https://doi.org/10.2323/jgam.2019.10.003","url":null,"abstract":"Glutaredoxins (Grxs) and thioredoxins (Trxs) play a critical role in resistance to oxidative conditions. However, physiological and biochemical roles of Mycoredoxin 3 (Mrx3) that shared a high amino acid sequence similarity to Grxs remain unknown in Corynebacterium glutamicum. Here we showed that mrx3 deletion strains of C. glutamicum was involved in the protection against oxidative stress. Recombinant Mrx3 not only catalytically reduced the disulfide bonds in ribonucleotide reductase (RNR), insulin and 5,5'-dithiobis-(2-nitro-benzoicacid) (DTNB), but also reduced the mixed disulphides between mycothiol (MSH) and substrate, which was exclusively linked to the thioredoxin reductase (TrxR) electron transfer pathway by a dithiol mechanism. Site-directed mutagenesis confirmed that the conserved Cys17 and Cys20 in Mrx3 were necessary to maintain its activity. The mrx3 deletion mutant showed decreased resistance to various stress, and these sensitive phenotypes were almost fully restored in the complementary strain. The physiological roles of Mrx3 in resistance to various stress were further supported by the induced expression of mrx3 under various stress conditions, directly under the control of the stress-responsive extracytoplasmic function-sigma (ECF-σ) factor SigH. Thus, we presented the first evidence that Mrx3 protected against various oxidative stresses by acting as a disulfide oxidoreductase behaving like Trx.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 4","pages":"125-133"},"PeriodicalIF":1.2,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38648177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The influence of medium composition on the microbial secretory production of hydroxyalkanoate oligomers. 培养基组成对微生物分泌羟基烷酸酯低聚物的影响。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-10-22 Epub Date: 2021-05-01 DOI: 10.2323/jgam.2020.09.002

Shoji Mizuno, Tetsuo Sakurai, Mikito Nabasama, Kyouhei Kawakami, Ayaka Hiroe, Seiichi Taguchi, Takeharu Tsuge

{"title":"The influence of medium composition on the microbial secretory production of hydroxyalkanoate oligomers.","authors":"Shoji Mizuno, Tetsuo Sakurai, Mikito Nabasama, Kyouhei Kawakami, Ayaka Hiroe, Seiichi Taguchi, Takeharu Tsuge","doi":"10.2323/jgam.2020.09.002","DOIUrl":"https://doi.org/10.2323/jgam.2020.09.002","url":null,"abstract":"With the aid of a chain transfer (CT) reaction, hydroxyalkanoate (HA) oligomers can be secreted by recombinant Escherichia coli carrying the gene encoding a lactate-polymerizing enzyme (PhaC1PsSTQK) in Luria-Bertani (LB) medium supplemented with a carbon source and CT agent. In this study, HA oligomers were produced through microbial secretion using a mineral-based medium instead of LB medium, and the impact of medium composition on HA oligomer secretion was investigated. The focused targets were medium composition and NaCl concentration related to osmotic conditions. It was observed that 4.21 g/L HA oligomer was secreted by recombinant E. coli in LB medium, but the amount secreted in the mineral-based modified R (MR) medium was negligible. However, when the MR medium was supplemented with 5 g/L yeast extract, 3.75 g/L HA oligomer was secreted. This can be accounted for by the enhanced expression and activity of PhaC1PsSTQK upon supplementation with growth-activated nutrients as supplementation with yeast extract also promoted cell growth and intracellular growth-associated polymer accumulation. Furthermore, upon adding 10 g/L NaCl to the yeast extract-supplemented MR medium, HA oligomer secretion increased to 6.86 g/L, implying that NaCl-induced osmotic pressure promotes HA oligomer secretion. These findings may facilitate the secretory production of HA oligomers using an inexpensive medium.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 4","pages":"134-141"},"PeriodicalIF":1.2,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38952037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

¹³C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae. 酿酒酵母菌甘油同化菌13c代谢通量分析

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-10-22 Epub Date: 2021-05-08 DOI: 10.2323/jgam.2020.10.001

Taiji Yuzawa, Tomokazu Shirai, Ryoko Orishimo, Kazuki Kawai, Akihiko Kondo, Takashi Hirasawa

{"title":"13C-metabolic flux analysis in glycerol-assimilating strains of Saccharomyces cerevisiae.","authors":"Taiji Yuzawa, Tomokazu Shirai, Ryoko Orishimo, Kazuki Kawai, Akihiko Kondo, Takashi Hirasawa","doi":"10.2323/jgam.2020.10.001","DOIUrl":"https://doi.org/10.2323/jgam.2020.10.001","url":null,"abstract":"Glycerol is an attractive raw material for the production of useful chemicals using microbial cells. We previously identified metabolic engineering targets for the improvement of glycerol assimilation ability in Saccharomyces cerevisiae based on adaptive laboratory evolution (ALE) and transcriptome analysis of the evolved cells. We also successfully improved glycerol assimilation ability by the disruption of the RIM15 gene encoding a Greatwall protein kinase together with overexpression of the STL1 gene encoding the glycerol/H+ symporter. To understand glycerol assimilation metabolism in the evolved glycerol-assimilating strains and STL1-overexpressing RIM15 disruptant, we performed metabolic flux analysis using 13C-labeled glycerol. Significant differences in metabolic flux distributions between the strains obtained from the culture after 35 and 85 generations in ALE were not found, indicating that metabolic flux changes might occur in the early phase of ALE (i.e., before 35 generations at least). Similarly, metabolic flux distribution was not significantly changed by RIM15 gene disruption. However, fluxes for the lower part of glycolysis and the TCA cycle were larger and, as a result, flux for the pentose phosphate pathway was smaller in the STL1-overexpressing RIM15 disruptant than in the strain obtained from the culture after 85 generations in ALE. It could be effective to increase flux for the pentose phosphate pathway to improve the glycerol assimilation ability in S. cerevisiae.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 4","pages":"142-149"},"PeriodicalIF":1.2,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38964568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effects of Enterococcus faecalis administration on the community structure of airborne bacteria in weanling piglet and layer hen houses. 粪肠球菌饲喂对断奶仔猪和蛋鸡舍空气细菌群落结构的影响。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-10-22 Epub Date: 2021-06-12 DOI: 10.2323/jgam.2020.11.001

Shoutao Cheng, Mo Chen, Min Gao, Tianlei Qiu, Shulei Tian, Shuyan Li, Xuming Wang

{"title":"Effects of Enterococcus faecalis administration on the community structure of airborne bacteria in weanling piglet and layer hen houses.","authors":"Shoutao Cheng, Mo Chen, Min Gao, Tianlei Qiu, Shulei Tian, Shuyan Li, Xuming Wang","doi":"10.2323/jgam.2020.11.001","DOIUrl":"https://doi.org/10.2323/jgam.2020.11.001","url":null,"abstract":"Probiotics have been shown to improve microbial compositions in animal intestine and feces, but the effects of probiotic administration on airborne microbial composition in animal houses remain unclear. In this study, we investigated the effects of dietary Enterococcus faecalis on the bacterial community structure in the air of piglet and layer hen houses. Indoor air and feces from piglet and layer hen houses were sampled after supplementing E. faecalis in feed for 60 days, and bacterial community structures were analyzed using Illumina high-throughput sequencing technology. Results showed that Chao1, ACE, Shannon, and Simpson indices of bacterial diversity did not significantly change in feces or indoor air of piglet or layer hen after supplementation with E. faecalis (P > 0.05). However, E. faecalis administration resulted in a decrease in the relative abundance of Proteobacteria (P < 0.05). In addition, E. faecalis significantly reduced the relative abundance of opportunistic pathogens such as Acinetobacter, Escherichia, and Shigella (P < 0.05), and beneficial bacterial genus such as Lactobacillus was significantly enriched in both feces and indoor air (P < 0.05). These changes should be of benefit to livestock, farm workers, and the surrounding environment.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 4","pages":"162-169"},"PeriodicalIF":1.2,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39008709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genome analysis and optimization of γ-aminobutyric acid (GABA) production by lactic acid bacteria from plant materials. 乳酸菌利用植物材料生产γ-氨基丁酸(GABA)的基因组分析及优化

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-10-22 Epub Date: 2021-06-05 DOI: 10.2323/jgam.2020.10.002

Sukanya Phuengjayaem, Nattakorn Kuncharoen, Auttaporn Booncharoen, Boonsri Ongpipattanakul, Somboon Tanasupawat

{"title":"Genome analysis and optimization of γ-aminobutyric acid (GABA) production by lactic acid bacteria from plant materials.","authors":"Sukanya Phuengjayaem, Nattakorn Kuncharoen, Auttaporn Booncharoen, Boonsri Ongpipattanakul, Somboon Tanasupawat","doi":"10.2323/jgam.2020.10.002","DOIUrl":"https://doi.org/10.2323/jgam.2020.10.002","url":null,"abstract":"Gamma-aminobutyric acid (GABA) plays a key role as an inhibitory neurotransmitter in the mammalian sympathetic nervous system and has other health benefits. Molecular characterization, genome analysis, and optimization were investigated to improve GABA production of a selected strain of lactic acid bacteria. Eleven isolates from plant materials were screened for GABA productivity and were identified based on phenotypic and genotypic characteristics. The most potent strain was chosen for genome analysis and GABA production optimization using the response surface methodology (RSM). Each of the two strains was closely related to Lactobacillus plantarum, Lactobacillus brevis, Weissella cibaria, Leuconostoc pseudomesenteroides while each strain was similar to Lactobacillus pentosus, Enterococcus, and Leuconostoc mesenteroides. They produced GABA ranging from 0.036 ± 0.000 to 17.315 ± 0.171 g/L at 72 h-cultivation. Among them, the most potent strain, SL9-6, showed the highest GABA production (17.315 g/L) when cultivated with 10% (v/v) inoculum for 48 h. The draft genome sequence of strain SL9-6 exhibited 96.90% average nucleotide identity value and 74.50% digital DNA-DNA hybridization to Lactobacillus brevis NCTC 13768T. This strain contained a glutamate decarboxylase gene system (gadA, gadB and gadC). Optimal culture conditions were determined as 40.00 g/L glucose, 49.90 g/L monosodium glutamate, pH 5.94, and 31.10°C by RSM, giving maximum GABA production of 32.48 g/L. Results from RSM also indicated that monosodium glutamate concentration, pH, and temperature were significant variables. GABA production significantly improved here could promise further application of strain SL9-6.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 4","pages":"150-161"},"PeriodicalIF":1.2,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38985766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6