Journal of General and Applied Microbiology最新文献_第10页

Glabridin synergy with norfloxacin induces ROS in multidrug resistant Staphylococcus aureus. 光甘草定与诺氟沙星协同作用诱导多重耐药金黄色葡萄球菌ROS。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-10-23 DOI: 10.2323/jgam.2021.06.002

Vigyasa Singh, Anirban Pal, Mahendra P Darokar

引用次数: 4

Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents. 本构细胞表面ZZ结构域的表达使酵母基免疫吸附剂的制备变得容易。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-08-07 DOI: 10.2323/jgam.2021.03.004

Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

引用次数: 4

Activation of cryptic milbemycin A₄ production in Streptomyces sp. BB47 by the introduction of a functional bldA gene. 引入功能性bldA基因激活链霉菌BB47中隐密的milbemycin A4生产。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-09-11 DOI: 10.2323/jgam.2021.04.001

Nana Matsui, Shizuka Kawakami, Dai Hamamoto, Sayuri Nohara, Reina Sunada, Watanalai Panbangred, Yasuhiro Igarashi, Takuya Nihira, Shigeru Kitani

{"title":"Activation of cryptic milbemycin A4 production in Streptomyces sp. BB47 by the introduction of a functional bldA gene.","authors":"Nana Matsui, Shizuka Kawakami, Dai Hamamoto, Sayuri Nohara, Reina Sunada, Watanalai Panbangred, Yasuhiro Igarashi, Takuya Nihira, Shigeru Kitani","doi":"10.2323/jgam.2021.04.001","DOIUrl":"https://doi.org/10.2323/jgam.2021.04.001","url":null,"abstract":"Streptomycetes are characterized by their ability to produce structurally diverse compounds as secondary metabolites and by their complex developmental life cycle, which includes aerial mycelium formation and sporulation. The production of secondary metabolites is growth-stage dependent, and generally coincides with morphological development on a solid culture. Streptomyces sp. BB47 produces several types of bioactive compounds and displays a bald phenotype that is devoid of an aerial mycelium and spores. Here, we demonstrated by genome analysis and gene complementation experiments that the bald phenotype arises from the bldA gene, which is predicted to encode the Leu-tRNAUUA molecule. Unlike the wild-type strain producing jomthonic acid A (1) and antarlide A (2), the strain complemented with a functional bldA gene newly produced milbemycin (3). The chemical structure of compound 3 was elucidated on the basis of various spectroscopic analyses, and was identified as milbemycin A4, which is an insecticidal/acaricidal antibiotic. These results indicate that genetic manipulation of genes involved in morphological development in streptomycetes is a valuable way to activate cryptic biosynthetic pathways.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 6","pages":"240-247"},"PeriodicalIF":1.2,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39408906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative stability of the folates highly accumulated in a non-Kyokai sake yeast. 非京海清酵母中高度积累的叶酸的定量稳定性。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-08-07 DOI: 10.2323/jgam.2021.03.002

Yusuke Shibata, Toshinari Takahashi, Tomoko Morimoto, Muneyoshi Kanai, Tsutomu Fujii, Takeshi Akao, Tetsuya Goshima, Tasuku Yamada

{"title":"Quantitative stability of the folates highly accumulated in a non-Kyokai sake yeast.","authors":"Yusuke Shibata, Toshinari Takahashi, Tomoko Morimoto, Muneyoshi Kanai, Tsutomu Fujii, Takeshi Akao, Tetsuya Goshima, Tasuku Yamada","doi":"10.2323/jgam.2021.03.002","DOIUrl":"https://doi.org/10.2323/jgam.2021.03.002","url":null,"abstract":"Pressed sake cake, a by-product of sake brewing, is a rich dietary source of folates, which are important vitamins for humans. However, considerable losses of folates occur during storage and cooking. We have previously reported that Km67, the house sake yeast strain of Kiku-masamune sake brewery, can accumulate high folate levels. In this study, we found that the folate content of pressed sake cakes produced with Km67 remained at approximately their maximum level after the fermentation activity stopped. To elucidate the mechanisms of high folate accumulation in Km67, we analyzed the expression of 23 folate-metabolizing genes. The expression of ABZ1 and FOL3 was almost always higher in Km67 than in Kyokai no. 701 yeast (K701), which suggested that enhanced expression of the genes involved in folate biosynthesis was a mechanism of high folate accumulation in Km67. We found that the folates of Km67 pressed sake cakes were quantitatively stable at 4°C under refrigerated storage conditions. In addition, the homocysteine content of Km67 pressed sake cakes was almost always higher than that of K701 pressed sake cakes. This result suggests that a reason for high folate accumulation in Km67 yeast is the need to reduce the intracellular concentration of homocysteine. Our results provide biologically meaningful information on folate metabolism in yeast.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"214-219"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39295870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis? 幽门螺杆菌感染是否影响慢性胃窦炎患者胃黏膜和胃液中的细菌结构?

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-05-29 DOI: 10.2323/jgam.2020.08.005

Yang Jiang, Fei Meng, Ying Liu, Liyun Zheng, Shufang Ye, Jianmei Zhang

{"title":"Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?","authors":"Yang Jiang, Fei Meng, Ying Liu, Liyun Zheng, Shufang Ye, Jianmei Zhang","doi":"10.2323/jgam.2020.08.005","DOIUrl":"https://doi.org/10.2323/jgam.2020.08.005","url":null,"abstract":"This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"179-185"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39047952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D. Vph1与D型新型隐球菌铜稳态有关。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-03 DOI: 10.2323/jgam.2021.02.001

Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara

{"title":"Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D.","authors":"Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara","doi":"10.2323/jgam.2021.02.001","DOIUrl":"https://doi.org/10.2323/jgam.2021.02.001","url":null,"abstract":"We clarified the roles of VPH1 in Cryptococcus neoformans serotype D by examining the detailed phenotypes of VPH1-deficient cells (Δvph1) in terms of their capability to grow in acidic and alkaline pH, at a high temperature, and under high osmotic conditions, in addition to the involvement of VPH1 in copper (Cu) homeostasis and the expression of some C. neoformans virulence factors. Δvph1 could grow well on minimal medium (YNB) but exhibited hypersensitivity to 20 μM Cu due to the failure to induce Cu-detoxifying metallothionein genes (CMT1 and CMT2). In contrast, Δvph1 exhibited defective growth on rich medium (YPD), and the induction of Cu transporter genes (CTR1 and CTR4) did not occur in this medium, implying that this strain was incapable of the uptake of Cu ions for growth. However, the addition of excess Cu promoted CTR gene expression and supported Δvph1 growth. These results suggested that the lack of the VPH1 gene disturbed Cu homeostasis in C. neoformans. Moreover, the loss of Vph1 function influenced the urease activity of C. neoformans.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"195-206"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome sequences of two strains of Lactococcus lactis subsp. cremoris with the same ancestry but a different capacity to produce exopolysaccharides. 两株乳酸乳球菌亚种的基因组序列分析。祖先相同但产生外多糖的能力不同的Cremoris。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-30 DOI: 10.2323/jgam.2021.03.001

Yayoi Gotoh, Kyosuke Kita, Kosei Tanaka, Shu Ishikawa, Toshio Suzuki, Ken-Ichi Yoshida

引用次数: 1

Identification, characterization, and cloning of a novel aminoacylase, L-pipecolic acid acylase from Pseudomonas species. 假单胞菌中l -胡椒酸酰化酶的鉴定、鉴定和克隆。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-06-26 DOI: 10.2323/jgam.2020.12.001

Junji Hayashi, Yoshiaki Ichiki, Akiko Kanda, Kazuyoshi Takagi, Mamoru Wakayama

{"title":"Identification, characterization, and cloning of a novel aminoacylase, L-pipecolic acid acylase from Pseudomonas species.","authors":"Junji Hayashi, Yoshiaki Ichiki, Akiko Kanda, Kazuyoshi Takagi, Mamoru Wakayama","doi":"10.2323/jgam.2020.12.001","DOIUrl":"https://doi.org/10.2323/jgam.2020.12.001","url":null,"abstract":"L-Pipecolic acid is utilized as a vital component of specific chemical compounds, such as immunosuppressive drugs, anticancer reagents, and anesthetic reagents. We isolated and characterized a novel L-aminoacylase, N-acetyl-L-pipecolic acid-specific aminoacylase (LpipACY), from Pseudomonas sp. AK2. The subunit molecular mass of LpipACY was 45 kDa and was assumed to be a homooctamer in solution. The enzyme exhibited high substrate specificity toward N-acetyl-L-pipecolic acid and a high activity for N-acetyl-L-pipecolic acid and N-acetyl-L-proline. This enzyme was stable at a high temperature (60°C for 10 min) and under an alkaline pH (6.0-11.5). The N-terminal and internal amino acid sequences of the purified enzyme were STTANTLILRNG and IMASGGV, respectively. These sequences are highly consistent with those of uncharacterized proteins from Pseudomonas species, such as amidohydrolase and peptidase. We also cloned and overexpressed the gene coding LpipACY in Escherichia coli. Moreover, the recombinant LpipACY exhibited properties similar to native enzyme. Our results suggest that LpipACY is a potential enzyme for the enzymatic synthesis of L-pipecolic acid. This study provides the first description of the enzymatic characterization of L-pipecolic acid specific amino acid acylase.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"186-194"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39111741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide. 蓝藻双球菌FPU101合成新分离多糖的生理和基因组分析及外多糖的化学分析。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-10 DOI: 10.2323/jgam.2021.02.002

Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki

{"title":"Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide.","authors":"Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki","doi":"10.2323/jgam.2021.02.002","DOIUrl":"https://doi.org/10.2323/jgam.2021.02.002","url":null,"abstract":"A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated. The isolate, named Chroococcus sp. FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1. Purified EPS from Chroococcus sp. FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7. The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM. However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS. The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp. FPU101, which was sequenced in this study. These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 5","pages":"207-213"},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Corynebacterium glutamicum Mycoredoxin 3 protects against multiple oxidative stresses and displays thioredoxin-like activity. 谷氨酰胺棒状杆菌核氧化还蛋白3可以抵抗多种氧化应激并显示类似硫氧还蛋白的活性。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-10-22 Epub Date: 2020-10-30 DOI: 10.2323/jgam.2019.10.003

Tao Su, Chengchuan Che, Ping Sun, Xiaona Li, Zhijin Gong, Jinfeng Liu, Ge Yang

{"title":"Corynebacterium glutamicum Mycoredoxin 3 protects against multiple oxidative stresses and displays thioredoxin-like activity.","authors":"Tao Su, Chengchuan Che, Ping Sun, Xiaona Li, Zhijin Gong, Jinfeng Liu, Ge Yang","doi":"10.2323/jgam.2019.10.003","DOIUrl":"https://doi.org/10.2323/jgam.2019.10.003","url":null,"abstract":"Glutaredoxins (Grxs) and thioredoxins (Trxs) play a critical role in resistance to oxidative conditions. However, physiological and biochemical roles of Mycoredoxin 3 (Mrx3) that shared a high amino acid sequence similarity to Grxs remain unknown in Corynebacterium glutamicum. Here we showed that mrx3 deletion strains of C. glutamicum was involved in the protection against oxidative stress. Recombinant Mrx3 not only catalytically reduced the disulfide bonds in ribonucleotide reductase (RNR), insulin and 5,5'-dithiobis-(2-nitro-benzoicacid) (DTNB), but also reduced the mixed disulphides between mycothiol (MSH) and substrate, which was exclusively linked to the thioredoxin reductase (TrxR) electron transfer pathway by a dithiol mechanism. Site-directed mutagenesis confirmed that the conserved Cys17 and Cys20 in Mrx3 were necessary to maintain its activity. The mrx3 deletion mutant showed decreased resistance to various stress, and these sensitive phenotypes were almost fully restored in the complementary strain. The physiological roles of Mrx3 in resistance to various stress were further supported by the induced expression of mrx3 under various stress conditions, directly under the control of the stress-responsive extracytoplasmic function-sigma (ECF-σ) factor SigH. Thus, we presented the first evidence that Mrx3 protected against various oxidative stresses by acting as a disulfide oxidoreductase behaving like Trx.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":"67 4","pages":"125-133"},"PeriodicalIF":1.2,"publicationDate":"2021-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38648177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2