Vph1与D型新型隐球菌铜稳态有关。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara
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引用次数: 0

摘要

我们通过检查VPH1缺陷细胞(Δvph1)在酸性和碱性pH、高温和高渗透条件下生长的能力的详细表型,以及VPH1参与铜(Cu)稳态和一些新生隐球菌毒力因子的表达,阐明了VPH1在新生隐球菌D血清型中的作用。Δvph1在微量培养基(YNB)上生长良好,但由于未能诱导铜解毒金属硫蛋白基因(CMT1和CMT2),对20 μM Cu表现出超敏反应。相反,Δvph1在富培养基(YPD)上表现出生长缺陷,并且在该培养基中没有发生Cu转运体基因(CTR1和CTR4)的诱导,这意味着该菌株不能吸收Cu离子进行生长。然而,过量Cu的添加促进了CTR基因的表达并支持Δvph1的生长。这些结果表明,缺乏VPH1基因扰乱了新生弓形虫的Cu稳态。此外,Vph1功能的缺失影响了新生C.的脲酶活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D.

We clarified the roles of VPH1 in Cryptococcus neoformans serotype D by examining the detailed phenotypes of VPH1-deficient cells (Δvph1) in terms of their capability to grow in acidic and alkaline pH, at a high temperature, and under high osmotic conditions, in addition to the involvement of VPH1 in copper (Cu) homeostasis and the expression of some C. neoformans virulence factors. Δvph1 could grow well on minimal medium (YNB) but exhibited hypersensitivity to 20 μM Cu due to the failure to induce Cu-detoxifying metallothionein genes (CMT1 and CMT2). In contrast, Δvph1 exhibited defective growth on rich medium (YPD), and the induction of Cu transporter genes (CTR1 and CTR4) did not occur in this medium, implying that this strain was incapable of the uptake of Cu ions for growth. However, the addition of excess Cu promoted CTR gene expression and supported Δvph1 growth. These results suggested that the lack of the VPH1 gene disturbed Cu homeostasis in C. neoformans. Moreover, the loss of Vph1 function influenced the urease activity of C. neoformans.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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