Journal of General and Applied Microbiology最新文献

Production of p-anisaldehyde via whole-cell transformation using recombinant E. coli expressing trans-anethole oxygenase.

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-18 DOI: 10.2323/jgam.2025.02.001

Zhikai Zhang, Qian Lin

{"title":"Production of p-anisaldehyde via whole-cell transformation using recombinant E. coli expressing trans-anethole oxygenase.","authors":"Zhikai Zhang, Qian Lin","doi":"10.2323/jgam.2025.02.001","DOIUrl":"https://doi.org/10.2323/jgam.2025.02.001","url":null,"abstract":"p-Anisaldehyde, a fragrance and flavour with important roles in food, cosmetics, and drug industries, is currently synthesized through chemical methods. Production of p-anisaldehyde by chemical oxidation of trans-anethole in industry gives rise to excessive by-products and adverse environmental impacts, whereas biological process would address such problems. Here, we presented a process of biotransformation of trans-anethole for production of p-anisaldehyde. The tao gene encoding for trans-anethole oxygenase (TAO) from Paraburkholderia sp. MR185 was fused with a solubilization tag GST and ProS2, respectively. GST did not exhibit solubility enhancement effect, whereas fusion with ProS2 significantly improved TAO's soluble expression in E. coli and the fusion protein ProS2-TAO-Sil3K accounted for more than 40% of total soluble proteins. ProS2-TAO-Sil3K was purified by simple silica affinity and its activity did not require addition of NADH, NADPH, and FAD. Metal ions Co2+, Zn2+, Ni2+, and Cu2+ displayed significant inhibition effect on TAO activity, and addition of Fe2+ improved enzyme activity by 32.6%. After induction, engineered E. coli cells were used as whole-cell biocatalyst for transformation of trans-anethole, and the final concentration of p-anisaldehyde reached 10.18 mM (1.38 g/L), with the volumetric productivity of 0.11 g/L/h and conversion rate of 67.9%. These results reveal that the biosynthesis of p-anisaldehyde has a great potential in practice.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of double bond configuration of 2-hydroxy-fatty acids and emendation of cellular fatty acid composition of Aureispira marina and Aureispira maritima. 确定 2-羟基脂肪酸的双键构型并修正金鱼藻和金鱼藻的细胞脂肪酸组成。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-13 Epub Date: 2024-09-26 DOI: 10.2323/jgam.2024.09.001

Fuka Iriyama, Hirokazu Iida, Kazuyoshi Kawahara

{"title":"Determination of double bond configuration of 2-hydroxy-fatty acids and emendation of cellular fatty acid composition of Aureispira marina and Aureispira maritima.","authors":"Fuka Iriyama, Hirokazu Iida, Kazuyoshi Kawahara","doi":"10.2323/jgam.2024.09.001","DOIUrl":"10.2323/jgam.2024.09.001","url":null,"abstract":"Aureispira marina is a marine bacterium with gliding motility isolated from the southern coastline of Thailand. It contained ceramide as a major cellular lipid composed of saturated or unsaturated branched chain 2-hydroxy-fatty acid and sphingosine. The structure of unsaturated 2-hydroxy-fatty acid was investigated in our previous study, but the geometric configuration of the double bond remained unclear. In the present study, 14-methyl-∆2-pentadecenol (∆2-iso-C16:1-ol) was prepared from D-2-hydroxy-15-methyl-∆3-hexadecenoic acid (D-2-OH-∆3-iso-C17:1) of the ceramide component, and analyzed by 1H and 13C NMR in comparison with ∆2-trans-hexadecenol (∆2-trans-n-C16:1-ol) derived from commercially available D-sphingosine. From the coupling constants of protons in the double bond and the chemical shift value of allylic carbon, the configuration of the double bond was determined as trans. Since the structure of 2-hydroxy-fatty acids was clarified, cellular fatty acids of A. marina and A. maritima, another species of the genus Aureispira, were reexamined, and the description on the cellular fatty acid composition of the genus Aureispira in the previous papers (Hosoya et al., 2006, Int. J. System. Evol. Microbiol., 56, 2931-2935; Hosoya et al., 2007, Int. J. System. Evol. Microbiol., 57, 1948-1951) lacking the description of 2-hydroxy-fatty acids was emended.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe. 一种含 DUF3844 结构域的蛋白质是酿酒酵母菌液泡蛋白分选所必需的。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-13 Epub Date: 2024-10-31 DOI: 10.2323/jgam.2024.10.001

Tomoaki Inagawa, Kazuma Ohkubo, Masahiro Watanabe, Tomotake Morita, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa

{"title":"A DUF3844 domain-containing protein is required for vacuolar protein sorting in Schizosaccharomyces pombe.","authors":"Tomoaki Inagawa, Kazuma Ohkubo, Masahiro Watanabe, Tomotake Morita, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa","doi":"10.2323/jgam.2024.10.001","DOIUrl":"10.2323/jgam.2024.10.001","url":null,"abstract":"Protein trafficking to vacuoles in plants and fungi, and to lysosomes in animals, is essential for the maintenance of cellular homeostasis. In Saccharomyces cerevisiae, the vacuolar protein sorting (VPS) pathway has been well studied by using vacuolar carboxypeptidase Y (CPY) as a model, and many VPS genes have been identified. By contrast, the vacuolar protein trafficking pathway in Schizosaccharomyces pombe remains poorly understood. In this study, we identified a novel VPS gene (SPBC1709.03) in S. pombe that is broadly conserved in fungi, but not in S. cerevisiae. Owing to its DUF3844 domain of unknown function, the gene was named vps3844. Disruption mutants of vps3844 had defects in both CPY sorting and incorporation of FM4-64 dye into the vacuolar membrane. Partial deletion analysis of the Vps3844 protein revealed that, within the DUF3844 domain, the region comprising amino acids 354 to 380 is important for protein trafficking to the vacuole. Our findings represent the first report of a VPS gene involved in vacuolar transport that is conserved in fungi, particularly S. pombe, but lacks representation in S. cerevisiae.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Bacterial Endophytes Associated with Cinchona ledgeriana Moens. and Their Potential in Plant-growth Promotion, Antifungal and Quinoline Alkaloids Production. Cinchona ledgeriana Moens.的内生细菌分离及其在促进植物生长、抗真菌和生产喹啉生物碱方面的潜力。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-13 Epub Date: 2024-10-25 DOI: 10.2323/jgam.2024.09.002

Fauzi Akhbar Anugrah, I Nyoman Pugeg Aryantha, Rahmi Masita, Siti Zubaidah, Nur Izzati Mohd Noh

{"title":"Isolation of Bacterial Endophytes Associated with Cinchona ledgeriana Moens. and Their Potential in Plant-growth Promotion, Antifungal and Quinoline Alkaloids Production.","authors":"Fauzi Akhbar Anugrah, I Nyoman Pugeg Aryantha, Rahmi Masita, Siti Zubaidah, Nur Izzati Mohd Noh","doi":"10.2323/jgam.2024.09.002","DOIUrl":"10.2323/jgam.2024.09.002","url":null,"abstract":"For centuries, quinoline alkaloids from the tree bark of Cinchona ledgeriana (C. ledgeriana) have been used in the treatment of malaria. However, unsustainable harvesting and poor growth conditions greatly limit its use as raw materials. Since plant endophytes are known to contribute to the physiology of the host and its metabolism for survival, this study showed the potential of endophytes isolated from C. ledgeriana roots in promoting the germination of Catharathus roseus (C. roseus) seedlings and the biosynthesis of quinoline alkaloid. In this present study, we found that the Enterobacteriaceae family comprised the majority of the bacterial community, with Klebsiella pneumoniae being the most abundant species at the C. ledgeriana roots. Characterization of culturable bacterial endophytes from the C. ledgeriana roots showed that all the isolates displayed plant growth-promoting factors and antifungal activities. Interestingly, chromatographic analyses led to the identification of the quinoline alkaloids producing Achromobacter xylosoxidans (A. xylosoxidans) A1. Moreover, the co-cultures of A. xylosoxidans A1, Cytobacillus solani (C. solani) A3, and Klebsiella aerogenes A6 increased the fresh and dry weight of the C. roseus seedlings. These results suggest that these bacterial endophytes may enhance quinine and quinidine production as well as the growth of the plant host.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial community analysis of sand filters used to treat mine water from a closed uranium mine. 对用于处理封闭铀矿矿井水的砂滤器进行微生物群落分析。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-13 Epub Date: 2024-09-12 DOI: 10.2323/jgam.2024.08.001

Hiroshi Habe, Tomohiro Inaba, Tomo Aoyagi, Hidenobu Aizawa, Yuya Sato, Tomoyuki Hori, Keiko Yamaji, Yoshiyuki Ohara, Kenjin Fukuyama, Takuro Nishimura

{"title":"Microbial community analysis of sand filters used to treat mine water from a closed uranium mine.","authors":"Hiroshi Habe, Tomohiro Inaba, Tomo Aoyagi, Hidenobu Aizawa, Yuya Sato, Tomoyuki Hori, Keiko Yamaji, Yoshiyuki Ohara, Kenjin Fukuyama, Takuro Nishimura","doi":"10.2323/jgam.2024.08.001","DOIUrl":"10.2323/jgam.2024.08.001","url":null,"abstract":"Rapid sand filters (RSFs) are employed in a drinking water treatment to remove undesirable elements such as suspended solids and dissolved metal ions. At a closed uranium (U) mine site, two sets of tandemly linked paired RSF systems (RSF1-RSF2 and RSF1-RSF3) were utilized to remove iron and manganese from mine water. In this study, a 16S rRNA-based amplicon sequencing survey was conducted to investigate the core microbes within the RSF system treating the mine water. In RSF1, two operational taxonomic units (OTUs) related to methanotrophic bacteria, Methylobacter tundripaludum (relative abundance: 18.1%) and Methylovulum psychrotolerans (11.5%), were the most and second most dominant species, respectively, alongside iron-oxidizing bacteria. The presence of these OTUs in RSF1 can be attributed to the microbial community in the inlet mine water, as the three most abundant OTUs in the mine water also dominated RSF1. Conversely, in both RSF2 and RSF3, Nevskia sp., previously isolated from the Ytterby mine manganese oxide producing ecosystem, became dominant, although known manganese-oxidizing bacterial OTUs were not detected. In contrast, a unique OTU related to Rhodanobacter sp. was the third most abundant (8.0%) in RSF1, possibly due to selective pressure from the radionuclide-contaminated environment during RSF operation, as this genus is known to be abundant at nuclear legacy waste sites. Understanding the key bacterial taxa in RSF system for mine water treatment could enhance the effectiveness of RSF processes in treating mine water from closed U mines.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

24R005A and 24R005B: Novel radical scavengers of DPPH obtained from Streptomyces sp. cultured in a fish powder medium. 24R005A 和 24R005B：从鱼粉培养基中培养的链霉菌中获得的新型 DPPH 自由基清除剂。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-13 Epub Date: 2024-07-31 DOI: 10.2323/jgam.2024.07.002

Toshikazu Komoda, Mayu Abe, Yoshitaka Koseki

引用次数: 0

Isolation and identification of naphtalene-degrading bacteria and its application in a two-phase partitioning bioreactor. 萘降解细菌的分离与鉴定及其在两相分离生物反应器中的应用

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-02-13 Epub Date: 2024-08-09 DOI: 10.2323/jgam.2024.07.003

Ran Deng, Jing Li, Bo Yu Liu, Jie Du, JianGuo Lu, Qiang Li, QianRu Hou

{"title":"Isolation and identification of naphtalene-degrading bacteria and its application in a two-phase partitioning bioreactor.","authors":"Ran Deng, Jing Li, Bo Yu Liu, Jie Du, JianGuo Lu, Qiang Li, QianRu Hou","doi":"10.2323/jgam.2024.07.003","DOIUrl":"10.2323/jgam.2024.07.003","url":null,"abstract":"Naphthalene is a persistent environmental pollutant for its potential teratogenic, carcinogenic and mutagenic effects. In this study, 10 strains of bacteria capable of degrading naphthalene were isolated from crude-oil contaminated soil. Among them, Pseudomonas plecoglossicida 2P exhibited prominent growth with 1000 mg/L naphthalene as the sole carbon source and degraded 94.15% of naphthalene in 36 h. Whole genome sequencing analysis showed that P. plecoglossicida 2P had a total of 22 genes related to naphthalene degradation, of which 8 genes were related to the salicylic acid pathway only, 5 genes were related to the phthalic acid pathway only, 8 genes were common in both the salicylic acid and phthalic acid pathways, and 1 gene was related to the gentisic acid pathway. P. plecoglossicida 2P was applied in a two-phase partition bioreactor (TPPB) to degrade naphthalene in wastewater. The optimal operating conditions of the reactor were obtained through response surface optimization: initial naphthalene concentration (C0) =1600 mg/L, bacterial liquid concentration (OD600) = 1.3, and polymer-to-wastewater mass ratio (PWR) = 2%. Under these conditions, the naphthalene degradation rate was 98.36% at 24 h. The degradation kinetics were fitted using the Haldane equation with a high coefficient of determination (R2=0.94). The present study laid foundations for naphthalene degradation mechanism of genus Pseudomonas and its potential application in TPPB.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening to isolate Bacillus subtilis mutants with enhanced NADPH levels. 提高NADPH水平的枯草芽孢杆菌突变体的筛选。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-01-16 DOI: 10.2323/jgam.2025.01.001

Yuzheng Wu, Shu Ishikawa, Ken-Ichi Yoshida

引用次数: 0

Characterization of the Azotobacter vinelandii nitrogenase complex expressed in Escherichia coli toward further activity improvement. 在大肠杆菌中表达的氮化固氮菌氮酶复合体的鉴定，以期进一步提高其活性。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2025-01-10 DOI: 10.2323/jgam.2024.12.001

Yusuke Ito, Daisuke Yoshidome, Makoto Hidaka, Yasuko Araki, Kotaro Ito, Saori Kosono, Makoto Nishiyama

引用次数: 0

Marine bacteria have multiple polyamide 4-degrading enzymes. 海洋细菌具有多种聚酰胺 4 降解酶。

IF 0.8 4区生物学

Journal of General and Applied Microbiology Pub Date : 2024-11-06 Epub Date: 2024-05-13 DOI: 10.2323/jgam.2024.05.001

Yusuke Saito, Ibuki Jin, Miwa Yamada

{"title":"Marine bacteria have multiple polyamide 4-degrading enzymes.","authors":"Yusuke Saito, Ibuki Jin, Miwa Yamada","doi":"10.2323/jgam.2024.05.001","DOIUrl":"10.2323/jgam.2024.05.001","url":null,"abstract":"Polyamide 4 (PA4) is expected to solve the issue of marine plastic pollution due to its excellent mechanical properties and biodegradability. In this study, to reveal the mechanism of PA4 biodegradation in the marine environment, we isolated 5 strains of PA4-degrading bacteria belonging to Aliiglaciecola, Dasania, and Pseudophaeobacter from a marine environment. The isolated 5 strains are novel PA4-degrading bacteria that are phylogenetically distinct from those isolated in previous studies. In addition, we compared the PA4-degrading activities and structures of the PA4-degrading enzymes secreted by the 5 strains and PA4-degrading strains isolated in our previous study. The PA4-degrading activity in the supernatant of the cultivation solutions differed among the strains. Native-PAGE and zymography using a polyacrylamide gel containing a PA4 emulsion demonstrated that PA4-degrading enzymes are classified into no less than three types of structures. These results suggested that marine PA4-degrading bacteria have multiple PA4-degrading enzymes. Our findings will contribute to a better understanding of the microbial degradation of PA4 in the marine environment.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0