Journal of General and Applied Microbiology最新文献_第9页

Involvement of a mycothiol-dependent reductase NCgl0018 in oxidative stress response of Corynebacterium glutamicum. 真菌硫醇依赖还原酶NCgl0018参与谷氨酸棒状杆菌氧化应激反应。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-09-06 DOI: 10.2323/jgam.2021.03.005

Keyan Chen, Xiaoyang Yu, Xinyu Zhang, Xiaona Li, Yang Liu, Meiru Si, Tao Su

{"title":"Involvement of a mycothiol-dependent reductase NCgl0018 in oxidative stress response of Corynebacterium glutamicum.","authors":"Keyan Chen, Xiaoyang Yu, Xinyu Zhang, Xiaona Li, Yang Liu, Meiru Si, Tao Su","doi":"10.2323/jgam.2021.03.005","DOIUrl":"https://doi.org/10.2323/jgam.2021.03.005","url":null,"abstract":"Corynebacterium glutamicum is an important industrial strain for amino acids and a key model organism for human pathogens. The study of C. glutamicum oxidoreductases, such as mycoredoxin 1 (Mrx1), dithiol-disulfide isomerase DsbA, and DsbA-like Mrx1, is helpful for understanding the survival, pathogenic infection, and stress resistance of its homologous species. However, the action mode and enzymatic function of C. glutamicum NCgl0018 preserving the Cys-Pro-Phe-Cys motif, annotated as a putative DsbA, have remained enigmatic. Here, we report that the NCgl0018-deleted strain increased sensitivity to various oxidative stresses. The ncgl0018 expression was induced in the stress-responsive extracytoplasmic function-sigma (ECF-σ) factor SigH- and organic peroxide- and antibiotic-sensing regulator (OasR)-dependent manner by stress. NCgl0018 reduced S-mycothiolated mixed disulfides and intramolecular disulfides via a monothiol-disulfide mechanism preferentially linking the mycothiol/mycothione reductase/NADPH electron pathway. Site-directed mutagenesis confirmed Cys107 was the resolving Cys residue, while Cys104 was the nucleophilic cysteine that was oxidized to a sulfenic acid and then could form an intramolecular disulfide bond with Cys107 or a mixed disulfide with mycothiol under stress. Biochemical analyses indicated that NCgl0018 lacked oxidase properties like the classical DsbA. Further, enzymatic rates and substrate preferences of NCgl0018 were highly similar to those of DsbA-like Mrx1. Collectively, our study presented the first evidence that NCgl0018 protected against stresses by functioning as a novel DsbA-like Mrx1 but not DsbA and Mrx1.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39384967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Characterization of mycosporine-like amino acids in the edible cyanobacterium Nostoc commune (Di Pi Cai) from China. 中国食用蓝藻藻中真菌孢素样氨基酸的鉴定。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-08-04 DOI: 10.2323/jgam.2021.03.003

Yang Wei, Takumi Nishiuchi, Toshio Sakamoto

引用次数: 1

Glabridin synergy with norfloxacin induces ROS in multidrug resistant Staphylococcus aureus. 光甘草定与诺氟沙星协同作用诱导多重耐药金黄色葡萄球菌ROS。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-10-23 DOI: 10.2323/jgam.2021.06.002

Vigyasa Singh, Anirban Pal, Mahendra P Darokar

引用次数: 4

Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents. 本构细胞表面ZZ结构域的表达使酵母基免疫吸附剂的制备变得容易。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-08-07 DOI: 10.2323/jgam.2021.03.004

Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

引用次数: 4

Activation of cryptic milbemycin A₄ production in Streptomyces sp. BB47 by the introduction of a functional bldA gene. 引入功能性bldA基因激活链霉菌BB47中隐密的milbemycin A4生产。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-09-11 DOI: 10.2323/jgam.2021.04.001

Nana Matsui, Shizuka Kawakami, Dai Hamamoto, Sayuri Nohara, Reina Sunada, Watanalai Panbangred, Yasuhiro Igarashi, Takuya Nihira, Shigeru Kitani

{"title":"Activation of cryptic milbemycin A4 production in Streptomyces sp. BB47 by the introduction of a functional bldA gene.","authors":"Nana Matsui, Shizuka Kawakami, Dai Hamamoto, Sayuri Nohara, Reina Sunada, Watanalai Panbangred, Yasuhiro Igarashi, Takuya Nihira, Shigeru Kitani","doi":"10.2323/jgam.2021.04.001","DOIUrl":"https://doi.org/10.2323/jgam.2021.04.001","url":null,"abstract":"Streptomycetes are characterized by their ability to produce structurally diverse compounds as secondary metabolites and by their complex developmental life cycle, which includes aerial mycelium formation and sporulation. The production of secondary metabolites is growth-stage dependent, and generally coincides with morphological development on a solid culture. Streptomyces sp. BB47 produces several types of bioactive compounds and displays a bald phenotype that is devoid of an aerial mycelium and spores. Here, we demonstrated by genome analysis and gene complementation experiments that the bald phenotype arises from the bldA gene, which is predicted to encode the Leu-tRNAUUA molecule. Unlike the wild-type strain producing jomthonic acid A (1) and antarlide A (2), the strain complemented with a functional bldA gene newly produced milbemycin (3). The chemical structure of compound 3 was elucidated on the basis of various spectroscopic analyses, and was identified as milbemycin A4, which is an insecticidal/acaricidal antibiotic. These results indicate that genetic manipulation of genes involved in morphological development in streptomycetes is a valuable way to activate cryptic biosynthetic pathways.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39408906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative stability of the folates highly accumulated in a non-Kyokai sake yeast. 非京海清酵母中高度积累的叶酸的定量稳定性。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-08-07 DOI: 10.2323/jgam.2021.03.002

Yusuke Shibata, Toshinari Takahashi, Tomoko Morimoto, Muneyoshi Kanai, Tsutomu Fujii, Takeshi Akao, Tetsuya Goshima, Tasuku Yamada

{"title":"Quantitative stability of the folates highly accumulated in a non-Kyokai sake yeast.","authors":"Yusuke Shibata, Toshinari Takahashi, Tomoko Morimoto, Muneyoshi Kanai, Tsutomu Fujii, Takeshi Akao, Tetsuya Goshima, Tasuku Yamada","doi":"10.2323/jgam.2021.03.002","DOIUrl":"https://doi.org/10.2323/jgam.2021.03.002","url":null,"abstract":"Pressed sake cake, a by-product of sake brewing, is a rich dietary source of folates, which are important vitamins for humans. However, considerable losses of folates occur during storage and cooking. We have previously reported that Km67, the house sake yeast strain of Kiku-masamune sake brewery, can accumulate high folate levels. In this study, we found that the folate content of pressed sake cakes produced with Km67 remained at approximately their maximum level after the fermentation activity stopped. To elucidate the mechanisms of high folate accumulation in Km67, we analyzed the expression of 23 folate-metabolizing genes. The expression of ABZ1 and FOL3 was almost always higher in Km67 than in Kyokai no. 701 yeast (K701), which suggested that enhanced expression of the genes involved in folate biosynthesis was a mechanism of high folate accumulation in Km67. We found that the folates of Km67 pressed sake cakes were quantitatively stable at 4°C under refrigerated storage conditions. In addition, the homocysteine content of Km67 pressed sake cakes was almost always higher than that of K701 pressed sake cakes. This result suggests that a reason for high folate accumulation in Km67 yeast is the need to reduce the intracellular concentration of homocysteine. Our results provide biologically meaningful information on folate metabolism in yeast.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39295870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D. Vph1与D型新型隐球菌铜稳态有关。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-03 DOI: 10.2323/jgam.2021.02.001

Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara

{"title":"Vph1 is associated with the copper homeostasis of Cryptococcus neoformans serotype D.","authors":"Noor Fatin Omar, Tria Widiasih Widiyanto, Setyowati Triastuti Utami, Masakazu Niimi, Kyoko Niimi, Akio Toh-E, Susumu Kajiwara","doi":"10.2323/jgam.2021.02.001","DOIUrl":"https://doi.org/10.2323/jgam.2021.02.001","url":null,"abstract":"We clarified the roles of VPH1 in Cryptococcus neoformans serotype D by examining the detailed phenotypes of VPH1-deficient cells (Δvph1) in terms of their capability to grow in acidic and alkaline pH, at a high temperature, and under high osmotic conditions, in addition to the involvement of VPH1 in copper (Cu) homeostasis and the expression of some C. neoformans virulence factors. Δvph1 could grow well on minimal medium (YNB) but exhibited hypersensitivity to 20 μM Cu due to the failure to induce Cu-detoxifying metallothionein genes (CMT1 and CMT2). In contrast, Δvph1 exhibited defective growth on rich medium (YPD), and the induction of Cu transporter genes (CTR1 and CTR4) did not occur in this medium, implying that this strain was incapable of the uptake of Cu ions for growth. However, the addition of excess Cu promoted CTR gene expression and supported Δvph1 growth. These results suggested that the lack of the VPH1 gene disturbed Cu homeostasis in C. neoformans. Moreover, the loss of Vph1 function influenced the urease activity of C. neoformans.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39147180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis? 幽门螺杆菌感染是否影响慢性胃窦炎患者胃黏膜和胃液中的细菌结构?

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-05-29 DOI: 10.2323/jgam.2020.08.005

Yang Jiang, Fei Meng, Ying Liu, Liyun Zheng, Shufang Ye, Jianmei Zhang

{"title":"Does Helicobacter pylori infection affect the structure of bacteria in the gastric mucosa and fluid in patients with chronic antral gastritis?","authors":"Yang Jiang, Fei Meng, Ying Liu, Liyun Zheng, Shufang Ye, Jianmei Zhang","doi":"10.2323/jgam.2020.08.005","DOIUrl":"https://doi.org/10.2323/jgam.2020.08.005","url":null,"abstract":"This study aimed to evaluate the composition of the gastric microbiota in the gastric mucosa and gastric fluid of patients with chronic antral gastritis. Specifically, we sought to determine whether Helicobacter pylori (Hp) infection changes the bacterial community in the gastric mucosa or alters the microbiota in the gastric fluid. The bacterial community at another site in the stomach was also investigated. DNA was extracted from 160 samples collected from 40 patients with chronic antral gastritis (20 Hp-positive and 20 Hp-negative cases). Three tissue samples of the gastric mucosa (gastric angle, body, and antral mucosa) and one tube of gastric fluid were collected from every patient. A 16S rRNA amplification library was created, and high-throughput sequencing was performed. A profile of the community composition was obtained using bioinformatics methods, including cluster, taxonomy, and diversity analyses. Analysis of the gastric bacterial community revealed that the community compositions of the gastric mucosa and gastric fluid of patients without Hp are similar to but show differences from those of Hp-positive patients. The microbiota in Hp-positive patients exhibited reduced microbial diversity, and the gastric fluid of these patients contained a small proportion of Hp. The richness of Leptotrichia in mucosal samples was greater than that in gastric fluid samples from Hp-negative patients with chronic antral gastritis. Hp changes the growth of other microbiota in the mucosa and affects the microbiota in the gastric fluid of patients with chronic antral gastritis. In addition to Hp, the presence of other bacteria might be related to the development of chronic antral gastritis.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39047952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genome sequences of two strains of Lactococcus lactis subsp. cremoris with the same ancestry but a different capacity to produce exopolysaccharides. 两株乳酸乳球菌亚种的基因组序列分析。祖先相同但产生外多糖的能力不同的Cremoris。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-30 DOI: 10.2323/jgam.2021.03.001

Yayoi Gotoh, Kyosuke Kita, Kosei Tanaka, Shu Ishikawa, Toshio Suzuki, Ken-Ichi Yoshida

引用次数: 1

Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide. 蓝藻双球菌FPU101合成新分离多糖的生理和基因组分析及外多糖的化学分析。

IF 1.2 4区生物学

Journal of General and Applied Microbiology Pub Date : 2021-11-25 Epub Date: 2021-07-10 DOI: 10.2323/jgam.2021.02.002

Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki

{"title":"Physiological and genomic analysis of newly-isolated polysaccharide synthesizing cyanobacterium Chroococcus sp. FPU101 and chemical analysis of the exopolysaccharide.","authors":"Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki","doi":"10.2323/jgam.2021.02.002","DOIUrl":"https://doi.org/10.2323/jgam.2021.02.002","url":null,"abstract":"A unicellular cyanobacterium that produces a large amount of exopolysaccharide (EPS) was isolated. The isolate, named Chroococcus sp. FPU101, grew between 20 and 30°C and at light intensities between 10 and 80 μmol m-2 s-1. Purified EPS from Chroococcus sp. FPU101 had a molecular size of 5.9 × 103 kDa and contained galactose, rhamnose, fucose, xylose, mannose, glucose, galacturonic acid, and glucuronic acid at a molar ratio of 17.2:15.9:14.1:11.0:9.6:9.5:13.0:9.7. The EPS content significantly increased when the NaCl concentration in the medium was increased from 1.7 to 100 mM. However, high NaCl concentrations did not significantly affect the molecular size or chemical composition of the EPS. The genes wza, wzb, wzc, wzx, wzy, and wzz that are involved in EPS synthesis were conserved in the genome of Chroococcus sp. FPU101, which was sequenced in this study. These results suggest that the Wzy-dependent pathway is potentially involved in EPS production in this organism.","PeriodicalId":15842,"journal":{"name":"Journal of General and Applied Microbiology","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2021-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39173089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2