PA0575 (RmcA)与铜绿假单胞菌PAO1中其他c-二gmp代谢蛋白相互作用。

IF 0.8 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of General and Applied Microbiology Pub Date : 2023-01-24 DOI:10.2323/jgam.2022.05.003

Yanxiang Yao, Naren Xi, E Hai, Xiaomin Zhang, Jiayi Guo, Zhi Lin, Weidong Huang

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引用次数: 0

摘要

c-二- gmp(双-(3,5)-环二鸟苷单磷酸)作为一种中心信号分子正成为细菌生理学研究的热点。铜绿假单胞菌PAO1基因组包含高度复杂的c-二gmp代谢基因，许多这些蛋白质已经被鉴定和研究。特别是，这些蛋白质的复杂网络正在出现。在本研究中，我们主要通过Bacteria-2-Hybrid实验，发现ggdefa - eal双蛋白PA0575 (RmcA)与另外两个双蛋白PA4601 (MorA)和PA4959 (FimX)相互作用。这些观察结果暗示了c-二gmp代谢蛋白相互作用的复杂性。因此，我们的工作为增加对c-di-GMP信号的理解提供了一条数据。

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PA0575 (RmcA) interacts with other c-di-GMP metabolizing proteins in Pseudomonas aeruginosa PAO1.

As a central signaling molecule, c-di-GMP (bis-(3,5)-cyclic diguanosine monophosphate) is becoming the focus for research in bacteria physiology. Pseudomonas aeruginosa PAO1 genome contains highly complicated c-di-GMP metabolizing genes and a number of these proteins have been identified and investigated. Especially, a sophisticated network of these proteins is emerging. In current study, mainly through Bacteria-2-Hybrid assay, we found PA0575 (RmcA), a GGDEF-EAL dual protein, to interact with two other dual proteins of PA4601 (MorA) and PA4959 (FimX). These observations imply the intricacy of c-di-GMP metabolizing protein interactions. Our work thus provides one piece of data to increase the understandings to c-di-GMP signaling.

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来源期刊

Journal of General and Applied Microbiology 生物-生物工程与应用微生物

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.