清酒工业酵母菌高乙酸乙酯产率FAS2-F1279Y (3836T>A)多态性的基因型分析

IF 0.8 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of General and Applied Microbiology Pub Date : 2023-01-24 DOI:10.2323/jgam.2022.05.001

Takashi Kuribayashi, Toshiki Sakurai, Akira Hatakeyama, Toshio Joh, Mitsuoki Kaneoke

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引用次数: 0

摘要

在酿酒酵母菌中，辛酸是通过脂肪酸合酶的作用由辛酸酯化而产生辛酸乙酯。最近的一项研究报道了一种酵母突变体，其脂肪酸合成酶(FAS2)基因的α亚基(F1279Y;3836T>A)产生大量的辛酸乙酯。本研究设计了两组引物(P1/P2和P3/P4)，分别包含NdeI酶和SspI酶的限制性内切位点，并建立了一种简单快速的聚合酶链反应-限制性片段长度多态性测定方法，以鉴定含有高羧酸乙酯产量相关的FAS2-F1279Y突变的酵母。

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Genotypic analysis of the FAS2-F1279Y (3836T>A) polymorphism conferring high ethyl caprylate productivity in industrial sake yeast strains.

In Saccharomyces cerevisiae, ethyl caprylate is produced by the esterification of caprylic acid, which is synthesized through the action of fatty acid synthase. A recent study reported a yeast mutant with a single nucleotide substitution in the alpha subunit of fatty acid synthase (FAS2) gene (F1279Y; 3836T>A) that produced large amounts of ethyl caprylate. Here, we designed two primer sets (P1/P2 and P3/P4) with mismatches that incorporate restriction sites for the enzymes NdeI and SspI, respectively and developed an easy and rapid polymerase chain reaction-restriction fragment length polymorphism assay to identify yeasts harboring the FAS2-F1279Y mutation associated with high ethyl caprylate productivity.

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来源期刊

Journal of General and Applied Microbiology 生物-生物工程与应用微生物

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.