本构细胞表面ZZ结构域的表达使酵母基免疫吸附剂的制备变得容易。

IF 0.8 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of General and Applied Microbiology Pub Date : 2021-12-31 Epub Date: 2021-08-07 DOI:10.2323/jgam.2021.03.004

Kohei Katsurada, Masahiro Tominaga, Misato Kaishima, Hiroko Kato, Toshihide Matsuno, Chiaki Ogino, Akihiko Kondo, Jun Ishii, Katsumi Takayama

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引用次数: 4

摘要

我们描述了一种新的表达盒，它可以在酵母细胞表面高效和组成性地表达来自金黄色葡萄球菌蛋白a的ZZ结构域，从而很容易制备酵母基免疫吸附剂。利用这个包含PGK1启动子、α-因子分泌信号和flo1锚定蛋白的表达盒，我们成功地构建了一种基于酵母的人血清白蛋白免疫吸附剂。

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Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents.

We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.

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来源期刊

Journal of General and Applied Microbiology 生物-生物工程与应用微生物

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.