Journal of Experimental Botany最新文献_第7页

In vivo biosensing of subcellular pyruvate pools reveals photosynthesis-dependent metabolite dynamics in Nicotiana benthamiana. 亚细胞丙酮酸池的活体生物传感揭示了烟草中依赖光合作用的代谢物动态。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae398

Jan Multhoff, Jan-Ole Niemeier, Ke Zheng, Magdiel Sheng Satha Lim, Pedro Barreto, Jule Meret Niebisch, Till Ischebeck, Markus Schwarzländer

{"title":"In vivo biosensing of subcellular pyruvate pools reveals photosynthesis-dependent metabolite dynamics in Nicotiana benthamiana.","authors":"Jan Multhoff, Jan-Ole Niemeier, Ke Zheng, Magdiel Sheng Satha Lim, Pedro Barreto, Jule Meret Niebisch, Till Ischebeck, Markus Schwarzländer","doi":"10.1093/jxb/erae398","DOIUrl":"10.1093/jxb/erae398","url":null,"abstract":"Pyruvate is central to metabolism across biology. It acts as a metabolic hub linking major pathways including glycolysis, the Krebs cycle, fermentation, and synthesis of amino acids, fatty acids, isoprenoids, and nucleotides. Even though the central role of pyruvate is well established biochemically, there is a remarkable gap in our understanding of how pyruvate levels behave within cells, where pyruvate is distributed across different compartments. Moreover, differential changes in pyruvate pools may occur rapidly upon changes in metabolic fluxes. Recently, this problem has been addressed by the development of a genetically encoded pyruvate biosensor to provide first insights into the pyruvate dynamics in animal cells. Here, we established in vivo biosensing of pyruvate in plants. We provided advanced characterization of the biosensor properties and demonstrated the functionality of the sensor in the cytosol, the mitochondria, and the chloroplasts of Nicotiana benthamiana epidermal cells. Finally, we harnessed the tool to investigate the impact of photosynthesis on pyruvate with unprecedented spatial and temporal resolution, revealing pronounced changes in subcellular pyruvate pools. While highlighting the current limitations of the biosensor, this study provides proof-of-concept for how the dynamics and regulation of central carbon metabolites can be revealed in living plant tissues.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7254-7266"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Irregular green netting of eggplant fruit peel: a domestication trait controlled by SmGLK2 with potential for fruit colour diversification. 茄子果皮的不规则绿色网纹：由SmGLK2控制的驯化性状，具有果实颜色多样化的潜力。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae355

Andrea Arrones, Silvia Manrique, Joaquin Gomis-Cebolla, Virginia Baraja-Fonseca, Mariola Plazas, Jaime Prohens, Ezio Portis, Lorenzo Barchi, Giovanni Giuliano, Pietro Gramazio, Santiago Vilanova

{"title":"Irregular green netting of eggplant fruit peel: a domestication trait controlled by SmGLK2 with potential for fruit colour diversification.","authors":"Andrea Arrones, Silvia Manrique, Joaquin Gomis-Cebolla, Virginia Baraja-Fonseca, Mariola Plazas, Jaime Prohens, Ezio Portis, Lorenzo Barchi, Giovanni Giuliano, Pietro Gramazio, Santiago Vilanova","doi":"10.1093/jxb/erae355","DOIUrl":"10.1093/jxb/erae355","url":null,"abstract":"The distribution of chlorophylls in eggplant (Solanum melongena) peel exhibits either a uniform pattern or an irregular green netting pattern. The latter, manifested as a gradient of dark green netting that is intensified in the proximal part of the fruit on a pale green background, is common in wild relatives and some eggplant landraces. Despite the selection of uniform chlorophylls during domestication, the netting pattern contributes to a greater diversity of fruit colours. Here, we used over 2300 individuals from different populations, including a multi-parent advanced generation inter-cross population for candidate genomic region identification, an F2 population for bulked segregant analysis by sequencing, and advanced backcrosses for edges-to-core fine-mapping, to identify SmGLK2 gene as responsible for the irregular netting in eggplant fruits. We also analysed the gene sequence of 178 S. melongena accessions and 22 wild relative species for tracing the evolutionary changes that the gene has undergone during domestication. Three different mutations were identified leading to the absence of netting. The main causative indel induces a premature stop codon disrupting the protein conformation and function, which was confirmed by western blot analysis and confocal microscopy observations. SmGLK2 has a major role in regulating chlorophyll biosynthesis in eggplant fruit peel.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7066-7078"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630072/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis KNS3 and its two homologs mediate endoplasmic reticulum-to-plasma membrane traffic of boric acid channels. 拟南芥 KNS3 及其两个同源物介导了硼酸通道从内质网到质膜的运输。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae380

Zhe Zhang, Shunsuke Nakamura, Arisa Yamasaki, Masataka Uehara, Shunsuke Takemura, Kohei Tsuchida, Takehiro Kamiya, Shuji Shigenobu, Katsushi Yamaguchi, Toru Fujiwara, Sumie Ishiguro, Junpei Takano

{"title":"Arabidopsis KNS3 and its two homologs mediate endoplasmic reticulum-to-plasma membrane traffic of boric acid channels.","authors":"Zhe Zhang, Shunsuke Nakamura, Arisa Yamasaki, Masataka Uehara, Shunsuke Takemura, Kohei Tsuchida, Takehiro Kamiya, Shuji Shigenobu, Katsushi Yamaguchi, Toru Fujiwara, Sumie Ishiguro, Junpei Takano","doi":"10.1093/jxb/erae380","DOIUrl":"10.1093/jxb/erae380","url":null,"abstract":"Membrane proteins targeted to the plasma membrane are first transported from the endoplasmic reticulum (ER) to the Golgi apparatus. This study explored the mechanisms controlling plasma membrane trafficking of the boric acid channel AtNIP5;1 from the ER. Imaging-based screening using transgenic Arabidopsis identified six mutants in which GFP-NIP5;1 was localized in the ER in addition to the plasma membrane. Genetic mapping and whole-genome resequencing identified the responsible gene in four among the six mutants as KAONASHI3 (KNS3)/SPOTTY1/IMPERFECTIVE EXINE FORMATION. Among the plasma membrane-localized proteins tested, NIP5;1 and its homolog NIP6;1 were retained in the ER of the kns3 mutants. Our genetic analysis further discovered that two homologs of KNS3, KNSTH1 and KNSTH2, were also involved in the ER exit of NIP5;1. In Arabidopsis protoplasts and tobacco leaves, mCherry-fused KNS3 localized to the ER and Golgi, whereas KNSTH2 localized to the ER. The cytosolic C-terminal tail of KNS3 contains amino acids important for Golgi-to-ER trafficking. Furthermore, the ER-to-Golgi trafficking of KNS3 depended on KNSTH1 and KNSTH2, and the accumulation of these three proteins in Arabidopsis roots depended on each other. We propose that KNS3, KNSTH1, and KNSTH2 function as a cargo-receptor complex mediating the ER exit of NIP5;1.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7046-7065"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11629988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drought-dependent regulation of cell coupling in Arabidopsis leaf epidermis requires plasmodesmal protein NHL12. 拟南芥叶表皮细胞耦合的干旱依赖性调控需要质膜蛋白 NHL12。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae370

Anam Ayyoub, Xiuyuan Yu, Xingjian Zhang, Chen Gao, Jiazhou Li, Shijiao Yin, Shaolin Chen, Johannes Liesche

{"title":"Drought-dependent regulation of cell coupling in Arabidopsis leaf epidermis requires plasmodesmal protein NHL12.","authors":"Anam Ayyoub, Xiuyuan Yu, Xingjian Zhang, Chen Gao, Jiazhou Li, Shijiao Yin, Shaolin Chen, Johannes Liesche","doi":"10.1093/jxb/erae370","DOIUrl":"10.1093/jxb/erae370","url":null,"abstract":"The cytoplasm of most plant cells is connected by membrane-lined cell wall channels, the plasmodesmata (PD). Dynamic regulation of sugar, hormone, and protein diffusion through PD is essential for plant development and stress responses. Understanding this regulation requires knowledge of factors and mechanisms that control PD permeability through the modulation of callose levels in the cell wall around PD openings. We investigated PD regulation in leaf epidermal cells in relation to drought stress in Arabidopsis. PD-mediated cell wall permeability was decreased by drought stress and the hormone abscisic acid (ABA), and we tested how this related to several PD-associated genes with drought-responsive expression. Mutants of NON-RACE SPECIFIC DISEASE RESISTANCE/HIN1 HAIRPIN-INDUCED-LIKE 12 (NHL12) showed relatively low PD permeability that was unaffected by drought or ABA treatment. Overexpression of NHL12 in Nicotiana benthamiana epidermal cells increased PD permeability. Moreover, we showed that NHL12 can potentially interact with the callose synthase regulator NHL3 and we explored the effect of NHL12 abundance and/or lower interface permeability on ABA signaling genes. Our results indicate that NHL12 is a drought-responsive negative regulator of PD callose levels and, thereby, interface permeability. Results are discussed in relation to PD function during drought stress and the regulation of intercellular transport.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7019-7030"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630015/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PagHAM4a-PagSCL21 and PagHAM4b-PagTCP20 modules positively regulate cambial activity and its differentiation into secondary xylem in poplar. PagHAM4a-PagSCL21和PagHAM4b-PagTCP20模块对杨树的木质部活动及其向次生木质部的分化具有正向调节作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae375

Pan Zhao, Qiulin Yu, Yumei He, Pengfang Sun, Huilin Wang, Xinyi Zhou, Yuting Su, Huihong Guo

{"title":"PagHAM4a-PagSCL21 and PagHAM4b-PagTCP20 modules positively regulate cambial activity and its differentiation into secondary xylem in poplar.","authors":"Pan Zhao, Qiulin Yu, Yumei He, Pengfang Sun, Huilin Wang, Xinyi Zhou, Yuting Su, Huihong Guo","doi":"10.1093/jxb/erae375","DOIUrl":"10.1093/jxb/erae375","url":null,"abstract":"Stem secondary xylem produced by cambial division and differentiation is the main source of tree biomass. Secondary xylem formation involves a complex transcriptional regulatory network; however, the underlying mechanism is still being explored. Here, we report that PagHAM4a and PagHAM4b are positive regulators of cambial differentiation into secondary xylem in hybrid poplar (Populus alba × Populus glandulosa clone 84K). Overexpression of PagHAM4a and PagHAM4b enhanced cambial activity and increased the number of secondary xylem cells in the stems of poplar. By contrast, single or double mutations of PagHAM4a and PagHAM4b generated by CRISPR/Cas9 decreased cambial activity, leading to a significant reduction of secondary xylem. Neither overexpression nor mutation of the two genes affected the size of vessels and fibers in xylem. Both PagHAM4a- and PagHAM4b-regulated gene networks were mainly centered at the stage when cambium had just initiated secondary growth, but the molecular networks regulated by the two genes were distinct. Further analysis revealed that PagSCL21 and PagTCP20 are direct targets of PagHAM4a and PagHAM4b, respectively, and their overexpression also promoted cambial differentiation into secondary xylem. Taken together, we identified two novel key regulatory modules in poplar, PagHAM4a-PagSCL21 and PagHAM4b-PagTCP20, which provide new insights into the mechanism of secondary xylem formation in trees.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7174-7189"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of PASTICCINO2 with Golgi anti-apoptotic proteins confers resistance to endoplasmic reticulum stress and is dependent on very-long-chain fatty acids. PASTICCINO2 与高尔基体抗凋亡蛋白相互作用，可抵御依赖于超长链脂肪酸的内质网压力。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae344

Xiao-Han Tang, Yan Zhou, Yu-Ting He, Wei Zhang, Xi Chen, Jing Tan, Kun Guo, Yu-Ting Liu, Shu-Heng Zhao, Yi-Qiu Ning, Yue Sun, Xiao-Fang Li

{"title":"Interaction of PASTICCINO2 with Golgi anti-apoptotic proteins confers resistance to endoplasmic reticulum stress and is dependent on very-long-chain fatty acids.","authors":"Xiao-Han Tang, Yan Zhou, Yu-Ting He, Wei Zhang, Xi Chen, Jing Tan, Kun Guo, Yu-Ting Liu, Shu-Heng Zhao, Yi-Qiu Ning, Yue Sun, Xiao-Fang Li","doi":"10.1093/jxb/erae344","DOIUrl":"10.1093/jxb/erae344","url":null,"abstract":"The endoplasmic reticulum (ER) is crucial for maintaining cell homeostasis because it is the primary site for synthesizing secreted and transmembrane proteins and lipids. The unfolded protein response (UPR) is activated to restore the homeostasis of the ER when it is under stress; however, the relationship between lipids and the ER stress response in plants is not well understood. Arabidopsis GOLGI ANTI-APOPTOTIC PROTEINS (GAAPs) are involved in resisting ER stress, and in this study, we found that PASTICCINO2 (PAS2), which is involved in very-long-chain fatty acid (VLCFA) synthesis, interacts with GAAPs and INOSITOL REQUIRING ENZYME 1. The pas2 single-mutant and the gaap1 pas2 and gaap2 pas2 double-mutants exhibited increased seedling damage and an impaired UPR response under chronic ER stress. Site mutation combined with genetic analysis revealed that the role of PAS2 in resisting ER stress depended on its VLCFA synthesis domain. VLCFA contents were increased under ER stress, and this required GAAPs. Exogenous VLCFAs partially restored the defect in the activation of the UPR caused by mutation of PAS2 or GAAP under chronic ER stress. Our findings demonstrate that the association of PAS2 with GAAPs confers plant resistance to ER stress by regulating VLCFA synthesis and the UPR. This provides a basis for further studies on the connection between lipids and cell-fate decisions under stress.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7267-7283"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Model forms for triose phosphate utilization-limited photosynthetic rates: implications for estimation of photorespiratory carbon export and the contribution of mesophyll conductance to photosynthesis. 不同的三糖-磷酸利用受限光合作用模型对估计光呼吸出碳量和叶绿体中层传导率的影响。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae369

Zhenxiang Zhou, Xinyou Yin

引用次数: 0

Bread and hummus: trait connectance and correlation pleiades in grain crops. 面包和鹰嘴豆泥：谷物作物的性状联系和相关褶皱。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae374

Victor O Sadras

引用次数: 0

Phloem: a missing link in understanding tree growth response in a changing environment. 韧皮部：了解树木在不断变化的环境中的生长反应的一个缺失环节。