Journal of Experimental Botany最新文献_第10页

The homeodomain leucine zipper protein RhHB22 promotes petal senescence by repressing ascorbic acid biosynthesis in rose. 同源结构域亮氨酸拉链蛋白 RhHB22 通过抑制玫瑰中抗坏血酸的生物合成促进花瓣衰老。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae503

Ping Li, Yuexuan Qiu, Rui Wang, Bingjie Zhang, Yanxing Ma, Xiaoming Sun, Junping Gao, Yunhe Jiang

{"title":"The homeodomain leucine zipper protein RhHB22 promotes petal senescence by repressing ascorbic acid biosynthesis in rose.","authors":"Ping Li, Yuexuan Qiu, Rui Wang, Bingjie Zhang, Yanxing Ma, Xiaoming Sun, Junping Gao, Yunhe Jiang","doi":"10.1093/jxb/erae503","DOIUrl":"10.1093/jxb/erae503","url":null,"abstract":"Premature petal senescence dramatically reduces flower quality and value. Ethylene and reactive oxygen species (ROS) are key players in accelerating rose petal senescence, but the molecular mechanism by which ethylene antagonizes ROS scavenging is not well understood. Here, we showed that ethylene reduces ascorbic acid (AsA) production, leading to the accumulation of ROS and hastening petal senescence. Ethylene treatment suppressed the expression of GDP-l-galactose phosphorylase 1 (RhGGP1), encoding the rate-controlling enzyme in AsA biosynthesis. A HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP) II transcription factor, RhHB22, directly bound to the promoter of RhGGP1 and inhibited its transcription. RhHB22 is induced by ethylene, and silencing of RhHB22 increased RhGGP1 expression and AsA production, resulting in reduced H2O2 accumulation and delayed petal senescence. Additionally, the delayed petal senescence symptoms of RhHB22-silenced plants were suppressed by silencing RhGGP1. Moreover, the expression of RhGGP1, which is suppressed by ethylene in wild-type petals, was significantly compromised in RhHB22-silenced petals. These findings uncover the transcriptional regulatory mechanism by which ethylene promotes ROS accumulation and petal senescence by inhibiting AsA biosynthesis, enhance our understanding of ethylene-induced petal senescence, and provide novel insights for improving the longevity of cut flowers.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1704-1717"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symbiotic nitrogen fixation: a launchpad for investigating old and new challenges. 共生固氮：研究新旧挑战的起点。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae510

Maurizio Chiurazzi, Giovanna Frugis, Lorella Navazio

引用次数: 0

Multiple gatekeeping steps in pollination lock species specificity. 授粉过程中的多个门控步骤锁定了物种特异性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae488

Yong-Jun He, Shuo Xu, Kai-Mei Zhang, Yang Zhang, Xiang-Jian Liu, Chen Liu

{"title":"Multiple gatekeeping steps in pollination lock species specificity.","authors":"Yong-Jun He, Shuo Xu, Kai-Mei Zhang, Yang Zhang, Xiang-Jian Liu, Chen Liu","doi":"10.1093/jxb/erae488","DOIUrl":"10.1093/jxb/erae488","url":null,"abstract":"In flowering plants, pollen grains must undergo a series of critical processes, including adhesion, hydration, and germination, which are dependent on the stigma, to develop a pollen tube. This pollen tube then penetrates the stigma to reach the internal tissues of pistil, facilitating the transport of non-motile sperm cells to the embryo sac for fertilization. However, a dry stigma, characterized by the absence of an exudate that typically envelops a wet stigma, functions as a multi-layered filter in adhesion, hydration, germination, and penetration that permits the acceptance of compatible pollen or tubes while rejecting incompatible ones, thereby protecting the embryo sac from ineffective fertilization and maintaining species specificity. Given the significance of these selective events, related research has consistently been at the forefront of reproductive studies, with notable advancements being made in recent times. In this review, we systematically synthesize the selective events and provide comprehensive, up-to-date summaries of occurrences on dry stigmas with a particular focus on the Brassicaceae family, following the chronological sequence of these events. Our objective is to update and elucidate the critical points within pollination, identify unresolved questions, and propose potential avenues for future research in other plant families.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1510-1523"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MpmiR319 promotes gemma/gemma cup formation in the liverwort Marchantia polymorpha. MpmiR319促进多形地茅gemma/gemma杯的形成。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/eraf148

Kazutaka Futagami, Masayuki Tsuzuki, Mika Yoshida, Yuichiro Watanabe

{"title":"MpmiR319 promotes gemma/gemma cup formation in the liverwort Marchantia polymorpha.","authors":"Kazutaka Futagami, Masayuki Tsuzuki, Mika Yoshida, Yuichiro Watanabe","doi":"10.1093/jxb/eraf148","DOIUrl":"https://doi.org/10.1093/jxb/eraf148","url":null,"abstract":"MicroRNAs (miRNAs) were initially detected as approximately 20-22 nt sequences in plants. For decades miRNA-mediated regulation of target gene expression was characterized in many cases. The sequences of miR159/319 family miRNAs are conserved among land plants. The roles of miR159/319 have been comprehensively characterized in development of dicot and monocot plants. However, its biological function in bryophytes remain enigmatic. A model bryophyte Marchantia polymorpha also encodes miR319 at two loci and expresses the miRNA. We used a CRISPR/Cas9 system to edit genome sequences at MpMIR319a and/or MpMIR319b loci. The mutant lines developed relatively few gemma cups and gemmae, suggesting that MpmiR319 targets MpRKD or MpR2R3-MYB21 transcripts and suppresses its expression. We constructed miR319-resistant MpRKD (mMpRKD) and MpR2R3-MYB21 (mMpR2R3-MYB21) by decreasing the complementarity to miR319. Introduction of mMpRKD resulted in gemma/gemma cup-less liverwort mutants, but mMpR2R3-MYB21 did not. Transcription-fusion constructs between MpRKD promoter andβglucuronidase showed the gene is expressed in the rim and bottom of gemma cups. We found that the mir319a/mir319b double mutant could form gemma cups but of different sizes in a unpredictable arrangement when planted on vermiculite. These results together suggest that miR319 guides the formation of gemma cups/gemmae in standard positions collaborating with MpRKD.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Millets for a sustainable future. 小米是可持续发展的未来。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae507

Arindam Ghatak, Iro Pierides, Roshan Kumar Singh, Rakesh K Srivastava, Rajeev K Varshney, Manoj Prasad, Palak Chaturvedi, Wolfram Weckwerth

{"title":"Millets for a sustainable future.","authors":"Arindam Ghatak, Iro Pierides, Roshan Kumar Singh, Rakesh K Srivastava, Rajeev K Varshney, Manoj Prasad, Palak Chaturvedi, Wolfram Weckwerth","doi":"10.1093/jxb/erae507","DOIUrl":"10.1093/jxb/erae507","url":null,"abstract":"Our current agricultural system faces a perfect storm-climate change, a burgeoning population, and unpredictable outbreaks such as COVID-19 which disrupt food production, particularly for vulnerable populations in developing countries. A paradigm shift in agriculture practices is needed to tackle these issues. One solution is the diversification of crop production. While ~56% of the plant-based protein stems from three major cereal crops (rice, wheat, and maize), underutilized crops such as millets, legumes, and other cereals are highly neglected by farmers and the research community. Millets are one of the most ancient and versatile orphan crops with attributes such as fast growing, high yielding, withstanding harsh environments, and rich in micronutrients such as iron and zinc, making them appealing to achieve agronomic sustainability. Here, we highlight the contribution of millet to agriculture and focus on the genetic diversity of millet, genomic resources, and next-generation omics and their applications under various stress conditions. Additionally, integrative omics technologies could identify and develop millets with desirable phenotypes having high agronomic value and mitigating climate change. We emphasize that biotechnological interventions, such as genome-wide association, genomic selection, genome editing, and artificial intelligence/machine learning, can improve and breed millets more effectively.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1534-1545"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11981904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols. 拟南芥核亚胺素：组蛋白乙酰转移酶GCN5通过功能硫醇调控的研究

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae514

Barbara De Smet, Xi Yang, Zuzana Plskova, Carmen Castell, Alvaro Fernández-Fernández, Avilien Dard, Jan Masood, Amna Mhamdi, Jingjing Huang, Didier Vertommen, Kai Xun Chan, Sébastien Pyr Dit Ruys, Joris Messens, Pavel I Kerchev, Frank Van Breusegem

{"title":"The nuclear sulfenome of Arabidopsis: spotlight on histone acetyltransferase GCN5 regulation through functional thiols.","authors":"Barbara De Smet, Xi Yang, Zuzana Plskova, Carmen Castell, Alvaro Fernández-Fernández, Avilien Dard, Jan Masood, Amna Mhamdi, Jingjing Huang, Didier Vertommen, Kai Xun Chan, Sébastien Pyr Dit Ruys, Joris Messens, Pavel I Kerchev, Frank Van Breusegem","doi":"10.1093/jxb/erae514","DOIUrl":"10.1093/jxb/erae514","url":null,"abstract":"In aerobic life forms, reactive oxygen species (ROS) are produced by the partial reduction of oxygen during energy-generating metabolic processes. In plants, ROS production increases during periods of both abiotic and biotic stress, severely overloading the antioxidant systems. Hydrogen peroxide (H2O2) plays a central role in cellular redox homeostasis and signalling by oxidizing crucial cysteines to sulfenic acid, which is considered a biologically relevant post-translational modification (PTM). Until now, the impact of the nucleus on cellular redox homeostasis has been relatively unexplored. The regulation of histone-modifying enzymes by oxidative PTMs at redox-sensitive cysteine or tyrosine residues is particularly intriguing because it allows the integration of redox signalling mechanisms with chromatin control of transcriptional activity. One of the most extensively studied histone acetyltransferases is the conserved GENERAL CONTROL NONDEPRESSIBLE 5 (GCN5) complex. This study investigated the nuclear sulfenome in Arabidopsis thaliana by expressing a nuclear variant of the Yeast Activation Protein-1 (YAP1) probe and identified 225 potential redox-active proteins undergoing S-sulfenylation. Mass spectrometry analysis further confirmed the S-sulfenylation of GCN5 at Cys293, Cys368, and Cys400, and their functional significance and impact on the GCN5 protein-protein interaction network were assessed using cysteine-to-serine mutagenesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1569-1584"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling shared genetic regulators of plant architectural and biomass yield traits in the Sorghum Association Panel. 揭示高粱植株结构和生物量产量性状的共同遗传调控因子。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/eraf012

Anuradha Singh, Linsey Newton, James C Schnable, Addie M Thompson

{"title":"Unveiling shared genetic regulators of plant architectural and biomass yield traits in the Sorghum Association Panel.","authors":"Anuradha Singh, Linsey Newton, James C Schnable, Addie M Thompson","doi":"10.1093/jxb/eraf012","DOIUrl":"10.1093/jxb/eraf012","url":null,"abstract":"Sorghum is emerging as an ideal genetic model for designing high-biomass bioenergy crops. Biomass yield, a complex trait influenced by various plant architectural characteristics, is typically regulated by numerous genes. This study aimed to dissect the genetic regulators underlying 14 plant architectural traits and 10 biomass yield traits in the Sorghum Association Panel across two growing seasons. We identified 321 associated loci through genome-wide association studies (GWAS), involving 234 264 single nucleotide polymorphisms (SNPs). These loci include genes with known associations to biomass traits, such as maturity, dwarfing (Dw), and leafbladeless1, as well as several uncharacterized loci not previously linked to these traits. We also identified 22 pleiotropic loci associated with variation in multiple phenotypes. Three of these loci, located on chromosomes 3 (S03_15463061), 6 (S06_42790178; Dw2), and 9 (S09_57005346; Dw1), exerted significant and consistent effects on multiple traits across both growing seasons. Additionally, we identified three genomic hotspots on chromosomes 6, 7, and 9, each containing multiple SNPs associated with variation in plant architecture and biomass yield traits. Chromosome-wise correlation analyses revealed multiple blocks of positively associated SNPs located near or within the same genomic regions. Finally, genome-wide correlation-based network analysis showed that loci associated with flowering, plant height, leaf traits, plant density, and tiller number per plant were highly interconnected with other genetic loci influencing plant architectural and biomass yield traits. The pyramiding of favorable alleles related to these traits holds promise for enhancing the future development of bioenergy sorghum crops.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1625-1643"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11981901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Redox signalling in the nucleus: shaping the epigenetic code. 细胞核中的氧化还原信号：形成表观遗传密码。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/eraf060

Luisa Maria Sandalio

引用次数: 0

Apomixis at high resolution: unravelling diplospory in Asteraceae. Apomixis at a high resolution: unravelling diplospory in Asteraceae.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae477

Letizia Cornaro, Camilla Banfi, Alex Cavalleri, Peter J van Dijk, Tatyana Radoeva, Mara Cucinotta, Lucia Colombo

引用次数: 0

Receptor kinase LecRK-I.9 regulates cell wall remodelling during lateral root formation in Arabidopsis. 受体激酶LecRK-I。9调控拟南芥侧根形成过程中细胞壁的重塑。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-09 DOI: 10.1093/jxb/erae520

Kevin Bellande, David Roujol, Josiane Chourré, Sophie Le Gall, Yves Martinez, Alain Jauneau, Denise Arico, Axel Mithöfer, Vincent Burlat, Elisabeth Jamet, Hervé Canut

{"title":"Receptor kinase LecRK-I.9 regulates cell wall remodelling during lateral root formation in Arabidopsis.","authors":"Kevin Bellande, David Roujol, Josiane Chourré, Sophie Le Gall, Yves Martinez, Alain Jauneau, Denise Arico, Axel Mithöfer, Vincent Burlat, Elisabeth Jamet, Hervé Canut","doi":"10.1093/jxb/erae520","DOIUrl":"10.1093/jxb/erae520","url":null,"abstract":"Assembling and remodelling the cell wall is essential for plant development. Cell wall dynamics are controlled by cell wall proteins, polysaccharide biosynthesis, and a variety of sensor and receptor systems. LecRK-I.9, an Arabidopsis thaliana plasma membrane-localized lectin receptor kinase, was previously shown to be involved in cell wall-plasma membrane contacts and to play roles in plant-pathogen interactions, but until now its role in development was not known. LecRK-I.9 is transcribed at a high level in root tissues including the pericycle. Comparative transcript profiling of a loss-of-function mutant versus the wild type identified LecRK-I.9 as a regulator of cell wall metabolism. Consistently, lecrk-I.9 mutants displayed an increased pectin methylesterification level correlated with decreased pectin methylesterase and increased polygalacturonase activities. Also, LecRK-I.9 negatively impacted lateral root development through the direct or indirect regulation of genes encoding (i) cell wall remodelling proteins during early events of lateral root initiation, and (ii) cell wall signalling peptides (CLE2 and CLE4) repressing lateral root emergence and growth. Furthermore, low nitrate reduced LecRK-I.9 expression in roots, particularly in the lateral root emergence zone: even in these conditions, the control of CLE2 and CLE4 expression is maintained. Altogether, the results show that LecRK-I.9 is a key player in negatively regulating both pre-branch site formation and lateral root emergence.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1718-1734"},"PeriodicalIF":5.6,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0