Journal of Experimental Botany最新文献_第10页

Quiescence and dormancy underpin plasticity and resilience: the virtue of being idle. 静止和休眠是可塑性和复原力的基础：闲置的美德。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-16 DOI: 10.1093/jxb/erae396

Michael J Considine

引用次数: 0

Stem cell quiescence and dormancy in plant meristems. 植物分生组织中的干细胞休眠和休眠。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-16 DOI: 10.1093/jxb/erae201

Ali Eljebbawi, Anika Dolata, Vivien I Strotmann, Yvonne Stahl

引用次数: 0

Beyond floral initiation: the role of flower bud dormancy in flowering time control of annual plants. 超越花期启动：花芽休眠在一年生植物花期控制中的作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-16 DOI: 10.1093/jxb/erae223

Steven Penfield

引用次数: 0

Expression of the grapevine anion transporter ALMT2 in Arabidopsis root decreases shoot Cl-/NO3- ratio under salt stress. 在拟南芥根中表达葡萄阴离子转运体 ALMT2 可降低盐胁迫下芽的 Cl-/NO3- 比率。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-15 DOI: 10.1093/jxb/erae416

Yue Wu, Sam W Henderson, Rob R Walker, Megan C Shelden, Matthew Gilliham

{"title":"Expression of the grapevine anion transporter ALMT2 in Arabidopsis root decreases shoot Cl-/NO3- ratio under salt stress.","authors":"Yue Wu, Sam W Henderson, Rob R Walker, Megan C Shelden, Matthew Gilliham","doi":"10.1093/jxb/erae416","DOIUrl":"https://doi.org/10.1093/jxb/erae416","url":null,"abstract":"Grapevines (Vitis vinifera, Vvi) are economically important crop plants which, when challenged with salt (NaCl) in soil and/or irrigation water, tend to accumulate Na+ and Cl- in aerial tissues impacting yield, and berry acceptability for winemaking. Grapevine (Vitis spp.) rootstocks vary in their capacity for shoot Cl- exclusion. Here, we characterise two putative anion transporter genes - Aluminium-activated Malate Transporter VviALMT2 and VviALMT8 - that were differentially expressed in the roots of efficient (140 Ruggeri) and inefficient (K51-40) Cl- excluding rootstocks, to explore their potential for impacting shoot Cl- exclusion. Using the Xenopus laevis oocyte expression system, VviALMT2 and VviALMT8 formed conductive channels that were highly permeable to NO3-, slightly-to-moderately permeable to other substrates including Cl- and malate, but impermeable to SO42-. RT-qPCR analyses revealed that VviALMT2 was more highly expressed in the root vasculature and up-regulated by high [NO3-] re-supply post starvation, while fluorescently tagged translational fusion VviALMT2 localised to the plasma membrane. As VviALMT8 showed no such features, we selected VviALMT2 as our salt exclusion candidate and assessed its function in planta. Expression of VviALMT2 in Arabidopsis thaliana root vasculature reduced shoot [Cl-]/[NO3-] after NaCl treatment, which suggests that VviALMT2 can be beneficial to plants under salt stress.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing photosynthetic phosphorus-use efficiency through coordination of leaf phosphorus fractions, allocation and anatomy during soybean domestication. 在大豆驯化过程中，通过协调叶片磷的组分、分配和解剖，提高光合作用磷的利用效率。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-13 DOI: 10.1093/jxb/erae427

Geng Chen, Qiu-Xia Ran, Cai Wang, Jiayin Pang, Ming-Jian Ren, Zheng-Yu Wang, Jin He, Hans Lambers

{"title":"Enhancing photosynthetic phosphorus-use efficiency through coordination of leaf phosphorus fractions, allocation and anatomy during soybean domestication.","authors":"Geng Chen, Qiu-Xia Ran, Cai Wang, Jiayin Pang, Ming-Jian Ren, Zheng-Yu Wang, Jin He, Hans Lambers","doi":"10.1093/jxb/erae427","DOIUrl":"https://doi.org/10.1093/jxb/erae427","url":null,"abstract":"Soybean domestication has significantly changed key agronomic traits, yet its impact on leaf photosynthetic phosphorus-use efficiency (PPUE) and its underlying traits remain poorly known. Further information on this would be important to increase soybean P-use efficiency. To address this gap, 48 soybean accessions (16 wild relatives, 16 landraces and 16 cultivars) were used to compare leaf anatomical traits, foliar chemical P fractions, P allocation and PPUE under two P levels. The results showed that the cultivars had higher area-based and mass-based photosynthesis rates, PPUE, metabolite P concentration, and its percentage of leaf total P, as well as a greater percentage of lipid P, nucleic acid P and residual P. Conversely, wild relatives tended to have higher leaf P concentration, palisade:spongy thickness ratio, and concentrations of inorganic P, nucleic acid P, lipid P and residual P. PPUE was negatively correlated with leaf inorganic P concentration and its percentage relative to leaf total P, while it was positively correlated with the concentration and percentage of metabolite P. We concluded that soybean domestication increased PPUE, as a result of both increased photosynthesis rate and decreased leaf P concentration; domestication reduced the palisade:spongy thickness ratio coupled with increased allocation of P to P-containing metabolites, thereby contributing to faster photosynthesis and higher PPUE. This study shed light on the significance of leaf P allocation and anatomical traits affecting PPUE during soybean domestication, offering a mechanistic understanding to further enhance soybean P-use efficiency.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grain Lysine enrichment and improved stress tolerance in rice through protein engineering. 通过蛋白质工程富集谷粒赖氨酸并提高水稻的抗逆性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-11 DOI: 10.1093/jxb/erae414

Ray Singh Rathore, Manjari Mishra, Ashwani Pareek, Sneh Lata Singla-Pareek

{"title":"Grain Lysine enrichment and improved stress tolerance in rice through protein engineering.","authors":"Ray Singh Rathore, Manjari Mishra, Ashwani Pareek, Sneh Lata Singla-Pareek","doi":"10.1093/jxb/erae414","DOIUrl":"https://doi.org/10.1093/jxb/erae414","url":null,"abstract":"Amino acids are a major source of nourishment for people living in regions where rice is a staple food. However, rice grain is deficient in essential amino acids, such as lysine. The activity of dihydrodipicolinate synthase (DHDPS) enzyme is crucial for lysine production in higher plants, but it is highly regulated through a feedback inhibition by its end product lysine, leading to its limited activity in the grain and resulting in low lysine accumulation. We identified lysine binding sites in the DHDPS enzyme and introduced key mutations to make it lysine feedback insensitive. Using in vivo analysis and functional complementation assays, we confirmed that protein engineering of the DHDPS renders it insensitive to lysine. Expression of mutated DHDPS resulted in 29 % higher lysine and 15 % higher protein accumulation in rice grains than the wild type. Importantly, the lysine content in transgenic grains was maintained in cooked rice. Further, the transgenic plants exhibited enhanced stress tolerance along with better antioxidant levels, improved photosynthesis, and higher grain yield compared to wild type plants. We have shown for the first time in rice that protein engineering of DHDPS can lead to accumulation of lysine in grains and impart abiotic stress tolerance. This approach could improve health in regions with nutrient deficiencies and environmental stressors that challenge food production and human health.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9 editing of two adenine phosphoribosyl transferase coding genes reveals the functional specialization of adenine salvage proteins in common bean. 对两个腺嘌呤磷酸核糖转移酶编码基因进行CRISPR/Cas9编辑，揭示了蚕豆中腺嘌呤挽救蛋白的功能特异性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-10 DOI: 10.1093/jxb/erae424

Cristina Mª López, Saleh Alseekh, Félix J Martínez Rivas, Alisdair R Fernie, Pilar Prieto, Josefa M Alamillo

{"title":"CRISPR/Cas9 editing of two adenine phosphoribosyl transferase coding genes reveals the functional specialization of adenine salvage proteins in common bean.","authors":"Cristina Mª López, Saleh Alseekh, Félix J Martínez Rivas, Alisdair R Fernie, Pilar Prieto, Josefa M Alamillo","doi":"10.1093/jxb/erae424","DOIUrl":"https://doi.org/10.1093/jxb/erae424","url":null,"abstract":"Adenine metabolism is important for common bean (Phaseolus vulgaris L) productivity since this legume uses ureides derived from the oxidation of purine nucleotides, as their primary nitrogen storage molecules. Purine nucleotides are produced from de novo synthesis or through salvage pathways. Adenine phosphoribosyl transferase (APRT) is the enzyme dedicated to adenine nucleobase salvage for nucleotide synthesis, but it also acts on the inactivation of cytokinin bases. In common bean, the APRT enzyme is encoded by four genes. Gene expression analysis, biochemical properties and subcellular location suggest functional differences among the common bean APRT isoforms. CRISPR/Cas9 targeted downregulation of two of the four PvAPRTs followed by metabolomics and physiological analyses of targeted hairy roots reveals that, although the two proteins have redundant functions, PvAPRT1 mostly participates in the salvage of adenine, whereas PvAPRT5 is the predominant form in the regulation of cytokinin homeostasis and stress responses with a high impact in root and nodule growth.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRI-Seed-Wizard: Combining Deep Learning Algorithms with Magnetic Resonance Imaging Enables Advanced Seed Phenotyping. 磁共振成像种子向导：将深度学习算法与磁共振成像相结合，实现先进的种子表型。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-09 DOI: 10.1093/jxb/erae408

Iaroslav Plutenko, Volodymyr Radchuk, Simon Mayer, Peter Keil, Stefan Ortleb, Steffen Wagner, Volker Lehmann, Hardy Rolletschek, Ljudmilla Borisjuk

{"title":"MRI-Seed-Wizard: Combining Deep Learning Algorithms with Magnetic Resonance Imaging Enables Advanced Seed Phenotyping.","authors":"Iaroslav Plutenko, Volodymyr Radchuk, Simon Mayer, Peter Keil, Stefan Ortleb, Steffen Wagner, Volker Lehmann, Hardy Rolletschek, Ljudmilla Borisjuk","doi":"10.1093/jxb/erae408","DOIUrl":"https://doi.org/10.1093/jxb/erae408","url":null,"abstract":"Evaluation of relevant seed traits is an essential part of most plant breeding and biotechnology programs. There is need for non-destructive, three-dimensional assessment of the morphometry, composition, and internal features of seeds. Here, we introduced a novel tool, MRI-Seed-Wizard, which integrates deep learning algorithms with non-invasive magnetic resonance imaging (MRI) for its use in the new domain - plant MRI. The tool enabled in vivo quantification of 23 grain traits, including volumetric parameters of inner seed structure. Several of these features cannot be assessed using conventional techniques, including X-ray computed tomography. MRI-Seed-Wizard was designed to automate the manual processes of identifying, labeling, and analyzing digital MRI data. We further provide advanced MRI protocols that allow the evaluation of multiple seeds simultaneously to increase throughput. The versatility of MRI-Seed-Wizard in seed phenotyping was demonstrated for wheat (Triticum aestivum) and barley (Hordeum vulgare) grains, and is applicable to a wide range of crop seeds. Thus, artificial intelligence, combined with the most versatile imaging modality - MRI, opens up new perspectives in seed phenotyping and crop improvement.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-scale phenotyping of senescence-related changes in roots of Rapeseed in response to nitrate limitation. 针对硝酸盐限制对油菜根系衰老相关变化的多尺度表型分析。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-09 DOI: 10.1093/jxb/erae417

Maxence James, Céline Masclaux-Daubresse, Thierry Balliau, Anne Marmagne, Fabien Chardon, Jacques Trouverie, Philippe Etienne

{"title":"Multi-scale phenotyping of senescence-related changes in roots of Rapeseed in response to nitrate limitation.","authors":"Maxence James, Céline Masclaux-Daubresse, Thierry Balliau, Anne Marmagne, Fabien Chardon, Jacques Trouverie, Philippe Etienne","doi":"10.1093/jxb/erae417","DOIUrl":"https://doi.org/10.1093/jxb/erae417","url":null,"abstract":"Root senescence remains largely unexplored. In this study, the temporality of the morphological, metabolic, and proteomic changes occurring with root aging were investigated, providing a comprehensive picture of the root senescence program. We found novel senescence-related markers for the characterization of the developmental stage of root tissues. The rapeseed root system is unique in that it consists of the taproot and lateral roots. Our study confirms that the taproot, which transiently accumulates large quantities of starch and proteins, is specifically dedicated to nutrient storage and remobilization, while the lateral roots are mainly dedicated to nutrient uptake. Proteomic data from the taproot and lateral roots highlight the different senescence-related events that control nutrient remobilization and nutrient uptake capacities. Both the proteome and enzyme activities revealed senescence-induced proteases and nucleotide catabolic enzymes that deserve attention as they may play important roles in nutrient remobilization efficiency in rapeseed roots. Taking advantage of publicly available transcriptomic and proteomic data on senescent Arabidopsis leaves, we have highlighted new lists of senescence-related proteins specific or common to root organs and/or leaves.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and biochemical analysis of lipid biosynthesis in Cyanophora paradoxa: Limited role of chloroplast in fatty acid synthesis. 蓝藻脂质生物合成的基因组和生化分析：叶绿体在脂肪酸合成中的作用有限。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-08 DOI: 10.1093/jxb/erae420

Naoki Sato, Eri Ikemura, Mana Uemura, Koichiro Awai

{"title":"Genomic and biochemical analysis of lipid biosynthesis in Cyanophora paradoxa: Limited role of chloroplast in fatty acid synthesis.","authors":"Naoki Sato, Eri Ikemura, Mana Uemura, Koichiro Awai","doi":"10.1093/jxb/erae420","DOIUrl":"https://doi.org/10.1093/jxb/erae420","url":null,"abstract":"Archaeplastida, a group of photosynthetic organisms with primary plastids, consists of green algae (plus plants), red algae, and glaucophytes. In contrast to green and red algae, information on lipids and lipid biosynthesis still needs to be included in the glaucophytes. The chloroplast is the site of photosynthesis and fatty acid synthesis in all photosynthetic organisms known to date. However, the genomic data of the glaucophyte Cyanophora paradoxa suggested the lack of acetyl CoA carboxylase and most components of fatty acid synthase in the chloroplast. Instead, multifunctional fatty acid synthase and acetyl CoA carboxylase are likely to reside in the cytosol. To examine this hypothesis, we measured fatty acid synthesis in isolated chloroplasts and whole cells using stable isotope labeling. The chloroplasts had very low activity of fatty acid synthesis, if any. Most processes of fatty acid synthesis, including elongation and desaturation, must be performed within the cytosol, and the fatty acids imported into the chloroplasts are assembled into the chloroplast lipids by the enzymes common to other algae and plants. Cyanophora paradoxa is a rare organism in which fatty acid synthesis and photosynthesis are not tightly linked. This could question the common origin of these two biosynthetic processes in Archaeplastida.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142390988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0