Journal of Experimental Botany最新文献_第10页

Genetically encoded biosensors as gateways to retrograde redox signalling in live plants. 基因编码的生物传感器作为活植物逆行氧化还原信号的通道。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf080

José M Ugalde, Andreas J Meyer

{"title":"Genetically encoded biosensors as gateways to retrograde redox signalling in live plants.","authors":"José M Ugalde, Andreas J Meyer","doi":"10.1093/jxb/eraf080","DOIUrl":"10.1093/jxb/eraf080","url":null,"abstract":"In eukaryotic cells, protein supply to organelles varies depending on the stage of development and, in particular, exposure to environmental challenges. Adequate protein supply in terms of quality and quantity relies on sophisticated retrograde signalling systems that enable appropriate responses to the respective stress situations. Among many other retrograde signals, reactive oxygen species, which are generated during the initial stress response, are thought to be involved in transduction of redox-related signals that may also involve multiple redox pairs such as NAD(P)H/NAD(P)+ and redox-active metabolites such as glutathione. Deciphering such signals requires detailed knowledge of their amplitudes and temporal and spatial dynamics. Genetically encoded biosensors based on fluorescent proteins have been developed for a number of different redox-related physiological parameters and can be monitored in living cells, tissues, and even whole plants using a variety of instruments adapted to the respective resolution requirements, thus opening gateways to retrograde signalling in plant cells. This review summarizes and critically evaluates current probes and devices used to monitor sensor fluorescence. It also outlines how biosensors can be used in combination with genetic and pharmacological approaches, to extract meaningful information and dissect the retrograde redox signalling systems in living plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3722-3737"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitro-oxidative nucleotide modifications in plants and associated microorganisms: signalling sensors or stress symptoms? 植物和相关微生物中的硝基氧化核苷酸修饰——信号传感器还是应激症状？

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf188

Jagna Chmielowska-Bąk, Ewa Sobieszczuk-Nowicka, Magdalena Arasimowicz-Jelonek

引用次数: 0

Hydrogen sulfide and protein persulfidation in plant stress signaling. 植物胁迫信号中的硫化氢和蛋白质过硫化。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf100

Mingjian Zhou, Yanjie Xie, Frank Van Breusegem, Jingjing Huang

{"title":"Hydrogen sulfide and protein persulfidation in plant stress signaling.","authors":"Mingjian Zhou, Yanjie Xie, Frank Van Breusegem, Jingjing Huang","doi":"10.1093/jxb/eraf100","DOIUrl":"10.1093/jxb/eraf100","url":null,"abstract":"Hydrogen sulfide (H2S) is increasingly recognized as a crucial signaling molecule in plants that plays key roles in regulating physiological processes and enhancing stress tolerance. This review provides an updated summary of H2S signaling in plant stress responses, and discusses its uptake from external environmental sources, its endogenous biosynthesis, and its broader functions in stress adaptation. We summarize the impact of H2S on plants under various stress conditions and review the mechanisms through which it mediates signaling functions, with a particular focus on H2S-mediated protein persulfidation. In addition, we provide an overview of the current understanding of protein persulfidation in regulating physiological processes and stress responses in plants, offering both a general discussion of its effects under different stress conditions and specific examples to highlight its significance. Finally, we review recent proteomic studies on protein persulfidation in plants, comparing the identified persulfidated proteins across studies and highlighting shared biological processes and pathways. This review aims to consolidate our current understanding of H2S signaling and its roles as mediated by protein persulfidation in plants, while also offering insights to inspire future research in this rapidly evolving field.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3738-3757"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Searching for nitroxyl modulators in Arabidopsis thaliana: a new paradigm of redox signaling in plants. 在拟南芥中寻找硝基调节剂——植物氧化还原信号的新范式。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/erae494

Sebastian Suarez, Jolanta Floryszak-Wieczorek, Ewa Sobieszczuk-Nowicka, Fabio Doctorovich, Magdalena Arasimowicz-Jelonek

{"title":"Searching for nitroxyl modulators in Arabidopsis thaliana: a new paradigm of redox signaling in plants.","authors":"Sebastian Suarez, Jolanta Floryszak-Wieczorek, Ewa Sobieszczuk-Nowicka, Fabio Doctorovich, Magdalena Arasimowicz-Jelonek","doi":"10.1093/jxb/erae494","DOIUrl":"10.1093/jxb/erae494","url":null,"abstract":"Through extensive research, nitroxyl (HNO) has emerged as a newly recognized redox signal in plant developmental and stress responses. The interplay between nitric oxide (●NO) and HNO entails a complex network of signaling molecules and regulatory elements sensitive to the environment-specific redox conditions. However, functional implications for HNO in cell signaling require more detailed studies, starting with identifying HNO-level switches. To obtain insight into possible physiologically relevant HNO modulators, we used real-time detection to examine HNO/●NO production triggered by selected plant-related compounds (PRCs), including non-protein amino acids, antioxidants, and phytohormones, both in vitro and in vivo in the model plant Arabidopsis thaliana. Hydrogen sulfide, ascorbic acid, and salicylic acid were identified as superior PRCs in driving HNO/●NO interconversion in the cellular medium, so that these PRCs could provide ubiquitous bioavailability of HNO in plants. Meanwhile, resistance-inducing compounds tended to down-regulate HNO in Arabidopsis leaves. The present study indicates that non-enzymatic HNO/●NO interconversion mediated by functionally important PRCs constitutes a significant route for controlling endogenous HNO concentrations, providing ubiquitous HNO bioavailability in plant cells. Moreover, concurrent HNO/●NO monitoring shows that the redox signals are highly integrated and create a redox code that can be translated into a specific cellular response.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3809-3822"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric oxide as an integral element in priming-induced tolerance and plant stress memory. 一氧化氮是引发诱导的耐受性和植物胁迫记忆的重要因素。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf033

Gábor Fejes, Tamás Bodor, Réka Szőllősi, Selahattin Kondak, Kinga Kutasi, Vasileios Fotopoulos, Zsuzsanna Kolbert

{"title":"Nitric oxide as an integral element in priming-induced tolerance and plant stress memory.","authors":"Gábor Fejes, Tamás Bodor, Réka Szőllősi, Selahattin Kondak, Kinga Kutasi, Vasileios Fotopoulos, Zsuzsanna Kolbert","doi":"10.1093/jxb/eraf033","DOIUrl":"10.1093/jxb/eraf033","url":null,"abstract":"The beneficial effects of priming technology are aimed at the promotion of growth and development and stress tolerance in plants. Different seed pre-treatment and vegetative priming approaches (osmotic, chemical, physical, hormonal, and redox treatments) increase the level of nitric oxide (NO) which is an active contributor to growth regulation and defence responses. On the other hand, seed pre-treatment or vegetative priming mainly with the NO donor, sodium nitroprusside (SNP), helps to mitigate different abiotic stresses such as salinity, cold, drought, and excess metals. The effect of SNP/NO covers the alleviation of stress-specific effects (e.g. reduction of cadmium uptake in the case of cadmium loading, or improvement of water balance in the case of drought), as well as general effects, such as alleviating oxidative stress. Seed pre-treatment or vegetative priming with SNP/NO up-regulates the activity of antioxidant enzymes and increases the amount of a wide range of non-enzymatic antioxidants. However, due to adverse effects of SNP, we urge the testing of other conventional NO donors (e.g. S-nitrosoglutathione) as well as new substances with more favourable properties (e.g. NO-releasing nanomaterials, plasmas, plasma-treated liquids, or combined donors) as seed pre-treating and vegetative priming materials. This review provides further suggestions on the methodology and future directions of fundamental research in relation to NO-associated pre-treatment and priming.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3669-3685"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mitochondrial thioredoxin system regulates the TCA cycle-derived metabolic fluxes toward the GS/GOGAT cycle in illuminated leaves. 线粒体硫氧还蛋白系统调节光照叶片中TCA循环衍生的GS/GOGAT循环代谢通量。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf125

Paulo V L Souza, Vicente T C B Alencar, Humaira Bahadar, Joaquim Albenisio G Silveira, Danilo M Daloso

{"title":"The mitochondrial thioredoxin system regulates the TCA cycle-derived metabolic fluxes toward the GS/GOGAT cycle in illuminated leaves.","authors":"Paulo V L Souza, Vicente T C B Alencar, Humaira Bahadar, Joaquim Albenisio G Silveira, Danilo M Daloso","doi":"10.1093/jxb/eraf125","DOIUrl":"10.1093/jxb/eraf125","url":null,"abstract":"Previous studies suggest that the synthesis of glutamate/glutamine is regulated by the mitochondrial thioredoxin (TRX) system. However, the mechanisms behind it remain unclear. Here, we demonstrated that the level of citrate and glutamate was higher in illuminated leaves from Arabidopsis mutants lacking the mitochondrial TRX o1 (trxo1) or both NADPH-dependent TRX reductases A/B (ntrab), that are found in the nucleus, cytosol, and mitochondria, when compared with the wild type (WT). Increased 13C-labelling in glutamate derived from [13C]pyruvate was observed in illuminated trxo1 and ntrab leaves, but not in the WT or in the microsomal trxh2 mutant. The lack of TRX o1 decreased the content and activity of glutamine synthetase (GS), which leads to a lower level of glutamine, and exacerbated the increases in GS activity triggered by high light, when compared with the WT. The level of glutamine was positively correlated with the percentage of the oxidized GS band. However, the GS redox status was unaltered in all mutants. Our results indicate that mitochondrial TRX mutants have higher metabolic fluxes from the tricarboxylic acid (TCA) cycle to the GS/glutamate synthase (GOGAT) cycle in vivo, probably associated with an increased substrate availability and by direct and indirect TRX-mediated mechanisms that regulate enzymes of both the TCA and GS/GOGAT cycle.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3872-3888"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Celebrating 75 years of the Journal of Experimental Botany (1950-2025). 庆祝《实验植物学杂志》创刊75周年（1950-2025）。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf295

John E Lunn

引用次数: 0

Functional interactions among H2O2, NO, H2S, and melatonin in the physiology, metabolism, and quality of horticultural Solanaceae. H2O2、NO、H2S和褪黑素在园艺茄科植物生理、代谢和品质中的功能相互作用

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/erae513

Francisco J Corpas, Rosa M Rivero, Luciano Freschi, José M Palma

{"title":"Functional interactions among H2O2, NO, H2S, and melatonin in the physiology, metabolism, and quality of horticultural Solanaceae.","authors":"Francisco J Corpas, Rosa M Rivero, Luciano Freschi, José M Palma","doi":"10.1093/jxb/erae513","DOIUrl":"10.1093/jxb/erae513","url":null,"abstract":"Cellular signaling is a key component of both intra- and intercellular communication, playing a crucial role in the development of higher plants as well as in their responses to environmental conditions of both abiotic and biotic origin. In recent decades, molecules such as hydrogen peroxide (H2O2), nitric oxide (NO), hydrogen sulfide (H2S), and melatonin have gained significant relevance in plant physiology and biochemistry due to their signaling functions and their interactions, forming a comprehensive cellular communication network. The Solanaceae family includes a group of horticultural crops of great global importance, for example tomatoes, eggplants, and peppers, which are of major agroeconomic significance due to their widespread cultivation and consumption. The primary objective of this review is to analyze the functions of this group of signaling molecules, particularly in these crops, and to explore how their exogenous application or the genetic manipulation of their endogenous concentration could serve as a promising biotechnological tool in the horticultural industry. This approach could help mitigate the negative effects of various external stress factors and even preserve the organoleptic quality of these fruits during post-harvest storage.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3634-3656"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen and derived reactive species in legume-rhizobia interactions: paradoxes and dual roles. 豆科植物-根瘤菌相互作用中的氧和衍生活性物质：悖论和双重作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf160

Eliane Meilhoc, Alexandre Boscari, Nicolas Pauly, Marc Lepetit, Pierre Frendo, Claude Bruand, Alain Puppo, Renaud Brouquisse

{"title":"Oxygen and derived reactive species in legume-rhizobia interactions: paradoxes and dual roles.","authors":"Eliane Meilhoc, Alexandre Boscari, Nicolas Pauly, Marc Lepetit, Pierre Frendo, Claude Bruand, Alain Puppo, Renaud Brouquisse","doi":"10.1093/jxb/eraf160","DOIUrl":"10.1093/jxb/eraf160","url":null,"abstract":"Biological nitrogen (N2) fixation between legumes and rhizobia is the result of a symbiosis characterized by the formation of a new organ, the nodule, in which the plants house and feed the bacteria. Oxygen (O2) concentration inside the nodule is very low (of the order of a few tens of nanomolar). The nitrogenase responsible for the reduction of atmospheric N2 to ammonia is irreversibly inhibited by traces of O2, while O2 is required for the overall process of N2 fixation which consumes high amounts of energy and reducing power. How is this paradox solved? The setting up of a physical and chemical O2 diffusion barrier, including the synthesis of numerous leghemoglobins, a class of hemoproteins with a very high O2 absorption capacity, was evidenced. However, why are so many leghemoglobin genes present when they appear to be mainly expressed in the same nodule zone? Furthermore, why do the bacterial symbionts contain multiple O2 sensors despite the existence of a very low O2 tension inside the nodule? On the other hand, reactive oxygen species (ROS), which play important metabolic and signaling roles in symbiosis, appear to act as Janus molecules, exhibiting opposite effects throughout symbiosis establishment and nodule life. We aim to provide possible answers to the questions asked and to highlight the dual roles of ROS in nodule development, functioning, and senescence.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3758-3773"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions. 发育和环境对vtc2依赖性叶片抗坏血酸积累和功能的影响。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-03 DOI: 10.1093/jxb/eraf035

Sandrine Kappel, Maureen J Frieboes, Ryo Yokoyama, Christian Kappel, Szilvia Z Tóth, Alisdair R Fernie, Peter Jahns, Nicholas Smirnoff, Fayezeh Aarabi, Ute Armbruster

{"title":"Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.","authors":"Sandrine Kappel, Maureen J Frieboes, Ryo Yokoyama, Christian Kappel, Szilvia Z Tóth, Alisdair R Fernie, Peter Jahns, Nicholas Smirnoff, Fayezeh Aarabi, Ute Armbruster","doi":"10.1093/jxb/eraf035","DOIUrl":"10.1093/jxb/eraf035","url":null,"abstract":"In nature, environmental conditions strongly fluctuate, frequently subjecting plants to periods of immediate photo-oxidative stress. The small molecule ascorbate allows plants to cope with such stress conditions. Ascorbate scavenges reactive oxygen species and enables the rapid and full induction of photoprotective non-photochemical quenching (NPQ). NPQ is dependent on zeaxanthin, which requires ascorbate as the electron donor during its synthesis by the violaxanthin de-epoxidase. The VTC2 gene encodes one of two isoforms of GDP-l-galactose phosphorylase, the rate-controlling enzyme of ascorbate biosynthesis. In the current study, by including a newly identified vtc2 allele, we found that loss of VTC2 depleted ascorbate mainly from the mature leaves and thereby limited NPQ specifically in this tissue. Growth in fluctuating light and controlled climate suppressed the slow NPQ induction phenotype of vtc2 mature leaves to some degree. This was concurrent with a constitutively higher accumulation of zeaxanthin under this condition. When plants were shifted to natural conditions, with strongly fluctuating light and temperature, the ascorbate-deficient mature leaves of vtc2 bleached. Together, our results reveal developmental and environmental effects on VTC2-dependent ascorbate accumulation and function.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3823-3833"},"PeriodicalIF":5.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12404783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0