Journal of Experimental Botany最新文献_第9页

Unravelling the impact of domestication on competitive ability in durum wheat: a phenotypic plasticity perspective. 揭示驯化对杜伦麦竞争能力的影响：表型可塑性视角。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae480

Taïna Lemoine, Cyrille Violle, Eric Antoine Gonzalez, Mathis Gaubert, Aline Rocher, Hélène Fréville, Florian Fort

{"title":"Unravelling the impact of domestication on competitive ability in durum wheat: a phenotypic plasticity perspective.","authors":"Taïna Lemoine, Cyrille Violle, Eric Antoine Gonzalez, Mathis Gaubert, Aline Rocher, Hélène Fréville, Florian Fort","doi":"10.1093/jxb/erae480","DOIUrl":"10.1093/jxb/erae480","url":null,"abstract":"The need to address the impact of domestication on plant traits is frequently highlighted in modern agriculture. It is often argued that domesticated plants have lost competitive ability due to reduced phenotypic plasticity. This study investigates whether domestication has affected competitive ability, functional trait values, and plasticity in durum wheat across 39 genotypes representing four key stages of domestication, from wild progenitors to modern elite varieties. Plants were grown in pots, both alone and in competition with the same neighbouring genotype. Biomass, and above- and belowground traits were measured at the end of the vegetative stage. Our results showed that the three domesticated groups lost less biomass in response to competition compared with their wild progenitors. All genotypes developed thinner leaves and thicker roots when grown with a neighbour. While wild progenitors exhibited the highest plasticity, this did not translate to a greater competitive ability. These findings challenge the theoretical expectation that domesticated plants are less suited for competition. Instead, they suggest that domesticated plants perform well in competitive environments and question the need to reintroduce wild traits to improve competitive ability.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1300-1313"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host-dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by diazotrophic Actinomycetota Frankia cluster-2. Frankia cluster-2 诱导的放线菌结节中依赖于宿主的氮输出专门代谢。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae446

Fede Berckx, Thanh Van Nguyen, Rolf Hilker, Daniel Wibberg, Kai Battenberg, Jörn Kalinowski, Alison Berry, Katharina Pawlowski

{"title":"Host-dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by diazotrophic Actinomycetota Frankia cluster-2.","authors":"Fede Berckx, Thanh Van Nguyen, Rolf Hilker, Daniel Wibberg, Kai Battenberg, Jörn Kalinowski, Alison Berry, Katharina Pawlowski","doi":"10.1093/jxb/erae446","DOIUrl":"10.1093/jxb/erae446","url":null,"abstract":"Frankia cluster-2 strains are diazotrophs that engage in root nodule symbiosis with actinorhizal plants of the Cucurbitales and the Rosales. Previous studies have shown that an assimilated nitrogen source, presumably arginine, is exported to the host in nodules of Datisca glomerata (Cucurbitales), while a different metabolite is exported in the nodules of Ceanothus thyrsiflorus (Rosales). To investigate if an assimilated nitrogen form is commonly exported to the host by cluster-2 strains, and which metabolite would be exported in Ceanothus, we analysed gene expression levels, metabolite profiles, and enzyme activities in nodules. We conclude that the export of assimilated nitrogen in symbiosis seems to be a common feature for Frankia cluster-2 strains, but the source of nitrogen is host dependent. The export of assimilated ammonium to the host suggests that 2-oxoglutarate is drawn from the tricarboxylic acid (TCA) cycle at a high rate. This specialized metabolism obviates the need for the reductive branch of the TCA cycle. We found that several genes encoding enzymes of central carbon and nitrogen metabolism were lacking in Frankia cluster-2 genomes: the glyoxylate shunt and succinate semialdehyde dehydrogenase. This led to a linearization of the TCA cycle, and we hypothesized that this could explain the low saprotrophic potential of Frankia cluster-2.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1164-1178"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid transfer protein VAS inhibits the hypersensitive response via reactive oxygen species signaling in Nicotiana benthamiana.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae473

Rina Koyama, Akira Suzuki, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba

{"title":"Lipid transfer protein VAS inhibits the hypersensitive response via reactive oxygen species signaling in Nicotiana benthamiana.","authors":"Rina Koyama, Akira Suzuki, Kouhei Ohnishi, Yasufumi Hikichi, Akinori Kiba","doi":"10.1093/jxb/erae473","DOIUrl":"10.1093/jxb/erae473","url":null,"abstract":"Lipid transfer proteins (LTPs) are small cysteine-rich soluble proteins that affect flower and seed development, cuticular wax deposition, and biotic and abiotic stress responses. We isolated an LTP-encoding gene homologous to LTPVAS in Nicotiana benthamiana and designated it LTP-VASCULAR TISSUE SIZE (NbLTPVAS). This gene was expressed in seeds, leaves, roots, and stems. Additionally, NbLTPVAS expression was induced by hypersensitive response (HR)-inducing agents. Cell death was accelerated and the phytopathogenic bacterial population decreased significantly in NbLTPVAS-silenced plants infected with the incompatible Ralstonia solanacearum strain 8107. The expression of HR marker gene hin1 in NbLTPVAS-silenced plants was markedly induced by R. solanacearum 8107, indicative of the acceleration of HR. HR cell death in NbLTPVAS-silenced plants was also promoted by the Agrobacterium-mediated expression of HR-inducing proteins including INF1, AvrA, and PopP1. Excessive production of reactive oxygen species (ROS) was detected in NbLTPVAS-silenced plants. The expression of NbrbohB (encoding a ROS-generating enzyme) also increased in NbLTPVAS-silenced plants, but the expression of the antioxidant enzyme-encoding genes NbSOD and NbAPX decreased. The silencing of both NbLTPVAS and NbrbohB adversely affected HR induction. Moreover, NbLTPVAS was secreted into the intercellular washing fluid. The transient expression of the full-length NbLTPVAS induced the expression of antioxidant genes, attenuated ROS production, and suppressed the induction of HR cell death. This is the first functional analysis of LTPVAS in plant-microbe interactions. Our study provides novel insights into the role of NbLTPVAS as a negative regulator of HR via ROS homeostasis in N. benthamiana.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1285-1299"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A model estimating the level of floral transition in olive trees exposed to warm periods during winter. 一个估算冬季处于温暖期的橄榄树花期过渡程度的模型。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae459

Ilan Smoly, Haim Elbaz, Chaim Engelen, Tahel Wechsler, Gal Elbaz, Giora Ben-Ari, Alon Samach, Tamar Friedlander

{"title":"A model estimating the level of floral transition in olive trees exposed to warm periods during winter.","authors":"Ilan Smoly, Haim Elbaz, Chaim Engelen, Tahel Wechsler, Gal Elbaz, Giora Ben-Ari, Alon Samach, Tamar Friedlander","doi":"10.1093/jxb/erae459","DOIUrl":"10.1093/jxb/erae459","url":null,"abstract":"Rising winter temperatures jeopardize the fruit yield of trees that require a prolonged and sufficiently cold winter to flower. Predicting the exact risk to different crop varieties is the first step in mitigating the harmful effects of climate change. This work focused on olive (Olea europaea)-a traditional crop in the Mediterranean basin in which flowering depends on the sufficiency of cold periods and the lack of warm ones during the preceding winter. A satisfactory quantitative model forecasting its expected flowering under natural temperature conditions is still lacking. The effect of different temperature regimes on olive flowering level and flowering gene expression was tested empirically. A modified 'dynamic model' describing the response of a putative flowering factor to the temperature signal was constructed. The crucial component of the model was an unstable intermediate, produced and degraded at temperature-dependent rates. The model accounts for the number of both cold and warm hours and also for their sequence. Empirical flowering and temperature data were applied to fit the model parameters, using numerical constrained optimization techniques; the model outcomes were successfully validated. The model accurately predicted low-to-moderate flowering under winters with warm periods and properly accounted for the effects of warm periods during winter.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1266-1284"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The selective estrogen receptor modulator clomiphene inhibits sterol biosynthesis in Arabidopsis thaliana. 选择性雌激素受体调节剂氯米芬抑制拟南芥固醇的生物合成

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae481

Qing Wang, Kjell De Vriese, Sandrien Desmet, Ren Wang, Markéta Luklová, Qianqian Liu, Jacob Pollier, Qing Lu, Sarah Schlag, Walter Vetter, Alain Goossens, Eugenia Russinova, Geert Goeminne, Danny Geelen, Tom Beeckman, Steffen Vanneste

{"title":"The selective estrogen receptor modulator clomiphene inhibits sterol biosynthesis in Arabidopsis thaliana.","authors":"Qing Wang, Kjell De Vriese, Sandrien Desmet, Ren Wang, Markéta Luklová, Qianqian Liu, Jacob Pollier, Qing Lu, Sarah Schlag, Walter Vetter, Alain Goossens, Eugenia Russinova, Geert Goeminne, Danny Geelen, Tom Beeckman, Steffen Vanneste","doi":"10.1093/jxb/erae481","DOIUrl":"10.1093/jxb/erae481","url":null,"abstract":"Sterols are produced via complex, multistep biosynthetic pathways involving similar enzymatic conversions in plants, animals, and fungi, yielding a variety of sterol metabolites with slightly different chemical properties to exert diverse and specific functions. A tremendously diverse landscape of sterols, and sterol-derived compounds can be found across the plant kingdom, determining a wide spectrum of functions. Resolving the underlying biosynthetic pathways is thus instrumental to understanding the function and use of these molecules. In only a few plants, sterol biosynthesis has been studied using mutants. In non-model species, a pharmacological approach is required. However, this relies on only a few inhibitors. Here, we investigated a collection of inhibitors of mammalian cholesterol biosynthesis to identify new inhibitors of plant sterol biosynthesis. We showed that imidazole-type fungicides, bifonazole, clotrimazole, and econazole, inhibited the obtusifoliol 14α-demethylase CYP51 in plants. Moreover, we found that the selective estrogen receptor modulator, clomiphene, inhibited sterol biosynthesis in part by inhibiting the plant-specific cyclopropyl-cycloisomerase CPI1. These results demonstrate that rescreening of inhibitors of animal sterol biosynthesis is an easy approach for identifying novel inhibitors of plant sterol biosynthesis. The molecules used in this study expand the range of inhibitors for studying and manipulating sterol biosynthesis in the plant kingdom.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1131-1146"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 13 (SPL13) together with SPL9 redundantly regulates wax biosynthesis under drought stress. SQUAMOSA启动子结合蛋白样13 （SPL13）与SPL9一起冗余调控干旱胁迫下蜡的生物合成。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae505

引用次数: 0

The LncNAT11-MYB11-F3'H/FLS module mediates flavonol biosynthesis to regulate salt stress tolerance in Ginkgo biloba. LncNAT11-GbMYB11-GbF3'H/GbFLS模块介导黄酮醇生物合成以调节银杏叶的耐盐胁迫性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae438

Sian Liu, Hanyue Zhang, Zhaolong Meng, Zhichao Jia, Fangfang Fu, Biao Jin, Fuliang Cao, Li Wang

{"title":"The LncNAT11-MYB11-F3'H/FLS module mediates flavonol biosynthesis to regulate salt stress tolerance in Ginkgo biloba.","authors":"Sian Liu, Hanyue Zhang, Zhaolong Meng, Zhichao Jia, Fangfang Fu, Biao Jin, Fuliang Cao, Li Wang","doi":"10.1093/jxb/erae438","DOIUrl":"10.1093/jxb/erae438","url":null,"abstract":"Flavonols are important secondary metabolites that enable plants to resist environmental stresses. Although MYB regulation of flavonol biosynthesis has been well studied, the long non-coding RNA (lncRNA)-MYB networks involved in regulating flavonol biosynthesis remain unknown. Ginkgo biloba is rich in flavonols, which are the most important medicinal components. Based on multi-omics data and phylogenetic trees, we identified GbMYB11 as a potential key transcription factor regulating flavonol biosynthesis. Overexpression and virus-induced gene silencing (VIGS) experiments confirmed that GbMYB11 acts as a pivotal positive regulator in flavonol biosynthesis. In the transcriptome of calli overexpressing GbMYB11, we identified significant up-regulation of GbF3'H and GbFLS in the flavonol biosynthetic pathway. Yeast one-hybrid and dual-luciferase assays demonstrated that GbMYB11 enhances the expression of GbF3'H and GbFLS by binding to their promoters. Interestingly, we identified LncNAT11, an antisense lncRNA complement to GbMYB11, which negatively regulates flavonol biosynthesis by repressing the expression of GbMYB11. Consequently, we established the LncNAT11-GbMYB11-GbF3'H/GbFLS module as a critical regulator of flavonol biosynthesis in G. biloba, and further elucidated that this module can mitigate the accumulation of reactive oxygen species by modulating flavonol biosynthesis during salt stress. These findings unveil a novel mechanism underlying flavonol biosynthesis and an lncRNA-MYB-mediated salt stress tolerance strategy in plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1179-1201"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142521991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histidine limitation alters plant development and influences the TOR network. 组氨酸限制改变了植物的发育并影响了 TOR 网络。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae479

Amandine Guérin, Caroline Levasseur, Aline Herger, Dominik Renggli, Alexandros Georgios Sotiropoulos, Gabor Kadler, Xiaoyu Hou, Myriam Schaufelberger, Christian Meyer, Thomas Wicker, Laurent Bigler, Christoph Ringli

{"title":"Histidine limitation alters plant development and influences the TOR network.","authors":"Amandine Guérin, Caroline Levasseur, Aline Herger, Dominik Renggli, Alexandros Georgios Sotiropoulos, Gabor Kadler, Xiaoyu Hou, Myriam Schaufelberger, Christian Meyer, Thomas Wicker, Laurent Bigler, Christoph Ringli","doi":"10.1093/jxb/erae479","DOIUrl":"10.1093/jxb/erae479","url":null,"abstract":"Plant growth depends on growth regulators, nutrient availability, and amino acid levels, all of which influence cell wall formation and cell expansion. Cell wall integrity and structures are surveyed and modified by a complex array of cell wall integrity sensors, including leucine-rich repeat (LRR)-extensins (LRXs) that bind RALF (rapid alkalinization factor) peptides with high affinity and help to compact cell walls. Expressing the Arabidopsis root hair-specific LRX1 without the extensin domain, which anchors the protein to the cell wall (LRX1ΔE14), has a negative effect on root hair development. The mechanism of this negative effect was investigated by a suppressor screen, which led to the identification of a sune (suppressor of dominant-negative LRX1ΔE14) mutant collection. The sune82 mutant was identified as an allele of HISN2, which encodes an enzyme essential for histidine biosynthesis. This mutation leads to reduced accumulation of histidine and an increase in several amino acids, which appears to have an effect on the TOR (target of rapamycin) network, a major controller of eukaryotic cell growth. It also represents an excellent tool to study the effects of reduced histidine levels on plant development, as it is a rare example of a viable partial loss-of-function allele in an essential biosynthetic pathway.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1085-1098"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The causal arrows from genotype, environment, and management to plant phenotype are double headed. 从基因型、环境和管理到植物表型的因果箭头是双头的。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae455

Victor O Sadras, Peter T Hayman

{"title":"The causal arrows from genotype, environment, and management to plant phenotype are double headed.","authors":"Victor O Sadras, Peter T Hayman","doi":"10.1093/jxb/erae455","DOIUrl":"10.1093/jxb/erae455","url":null,"abstract":"Cause-and-effect arrows are drawn from genotype (G), environment (E), and agronomic management (M) to the plant phenotype in crop stands in a useful but incomplete framework that informs research questions, experimental design, statistical analysis, data interpretation, modelling, and breeding and agronomic applications. Here we focus on the overlooked bidirectionality of these arrows. The phenotype-to-genotype arrow includes increased mutation rates in stressed phenotypes, relative to basal rates. From a developmental viewpoint, the phenotype modulates gene expression, returning multiple cellular phenotypes with a common genome. The phenotype-to-environment arrow is captured in the process of niche construction, which spans from persistent and global to transient and local. Research on crop rotations recognizes the influence of the phenotype on the environment but is divorced from niche construction theory. The phenotype-to-management arrow involves, for example, a diseased crop that may trigger fungicide treatment. Making explicit the bidirectionality of the arrows in the G×E×M framework contributes to narrowing the gap between data-driven technologies and integrative theory, and is an invitation to think cautiously of the internal teleonomy of plants in contrast to the view of the phenotype as the passive end of the arrows in the current framework.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"917-930"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating biological nitrogen fixation via single-cell transcriptomics. 通过单细胞转录组学研究生物固氮。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae454

Wendell J Pereira, Daniel Conde, Noé Perron, Henry W Schmidt, Christopher Dervinis, Rafael E Venado, Jean-Michel Ané, Matias Kirst

{"title":"Investigating biological nitrogen fixation via single-cell transcriptomics.","authors":"Wendell J Pereira, Daniel Conde, Noé Perron, Henry W Schmidt, Christopher Dervinis, Rafael E Venado, Jean-Michel Ané, Matias Kirst","doi":"10.1093/jxb/erae454","DOIUrl":"10.1093/jxb/erae454","url":null,"abstract":"The extensive use of nitrogen fertilizers has detrimental environmental consequences, and it is essential for society to explore sustainable alternatives. One promising avenue is engineering root nodule symbiosis, a naturally occurring process in certain plant species within the nitrogen-fixing clade, into non-leguminous crops. Advancements in single-cell transcriptomics provide unprecedented opportunities to dissect the molecular mechanisms underlying root nodule symbiosis at the cellular level. This review summarizes key findings from single-cell studies in Medicago truncatula, Lotus japonicus, and Glycine max. We highlight how these studies address fundamental questions about the development of root nodule symbiosis, including the following findings: (i) single-cell transcriptomics has revealed a conserved transcriptional program in root hair and cortical cells during rhizobial infection, suggesting a common infection pathway across legume species; (ii) characterization of determinate and indeterminate nodules using single-cell technologies supports the compartmentalization of nitrogen fixation, assimilation, and transport into distinct cell populations; (iii) single-cell transcriptomics data have enabled the identification of novel root nodule symbiosis genes and provided new approaches for prioritizing candidate genes for functional characterization; and (iv) trajectory inference and RNA velocity analyses of single-cell transcriptomics data have allowed the reconstruction of cellular lineages and dynamic transcriptional states during root nodule symbiosis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"931-949"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0