Journal of Experimental Botany最新文献_第9页

CitACOS5, a fatty acyl-CoA synthetase, is crucial for male fertility in citrus by influencing pollen exine formation. CitACOS5通过影响花粉外皮的形成，对柑桔雄性育性起着至关重要的作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf181

Yan-Jie Fan, Feng-Quan Tan, Xing-Yi He, Zi-Ang Liu, Hui-Zhong Bai, Nan Jiang, Hui-Hui Jia, Wen-Hui Yang, Xin Song, Miao Zhang, Kai-Dong Xie, Wen-Wu Guo, Xiao-Meng Wu

{"title":"CitACOS5, a fatty acyl-CoA synthetase, is crucial for male fertility in citrus by influencing pollen exine formation.","authors":"Yan-Jie Fan, Feng-Quan Tan, Xing-Yi He, Zi-Ang Liu, Hui-Zhong Bai, Nan Jiang, Hui-Hui Jia, Wen-Hui Yang, Xin Song, Miao Zhang, Kai-Dong Xie, Wen-Wu Guo, Xiao-Meng Wu","doi":"10.1093/jxb/eraf181","DOIUrl":"10.1093/jxb/eraf181","url":null,"abstract":"Male sterility (MS) holds great potential and value for breeding seedless cultivars of fruit trees. However, functional characterization of MS-related genes has been difficult in perennial fruit crops, because of the extended juvenile period and difficulties in genetic transformation. In this study, we compared anther development of 'Qianyang Seedless' (QS), a natural seedless bud sport (somatic mutation) of Ponkan mandarin (Citrus reticulata Blanco) with a stable MS phenotype, with that of the seedy wild-type 'Egan No. 1' (EG). We observed significant abnormalities in QS at the microspore mother cell stage, leading to the generation of abnormal tetrads and non-viable pollen. A transcriptomic analysis of anthers at the microspore mother cell stage showed a significant down-regulation of the sporopollenin biosynthesis pathway genes in QS. Among them, CitACOS5 showed pronounced down-regulation in QS. CRISPR/Cas9 mutants of Mini-citrus (Fortunella hindsii), cr-acos5, exhibited complete MS phenotypes, characterized by the absence of pollen grains due to defects in pollen wall formation and tapetum degradation. Biochemical assays validated that CitMYB80 directly bound to the promoter of CitACOS5 and activated its expression. Our results suggest that CitACOS5 is crucial for male fertility in citrus by influencing pollen exine formation, which offers potential target genes for seedless citrus breeding.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4027-4042"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A DUF630 and 632 domains-containing protein, ZmNRL1, acts as a positive regulator of nitrogen stress response in maize. 玉米DUF630和632结构域蛋白ZmNRL1在氮胁迫响应中的正调控作用

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf202

Chunyan Zheng, Hanjie Li, Yanfei Liu, Xiner Huang, Junting Han, Na Luo, Faqiang Li

{"title":"A DUF630 and 632 domains-containing protein, ZmNRL1, acts as a positive regulator of nitrogen stress response in maize.","authors":"Chunyan Zheng, Hanjie Li, Yanfei Liu, Xiner Huang, Junting Han, Na Luo, Faqiang Li","doi":"10.1093/jxb/eraf202","DOIUrl":"10.1093/jxb/eraf202","url":null,"abstract":"Nitrogen (N) is a key macronutrient whose availability often determines maize growth and productivity. Improving nitrogen use efficiency (NUE) is critical to increase maize yield while reducing N input and, more importantly, to alleviate environmental pollution. However, only a few genes have been exploited for maize NUE improvement thus far. Here, we identified 44 candidate genes associated with NUE-related traits by performing a genome-wide association analysis in a maize natural population. We further found that the natural variations in ZmNRL1, encoding a DUF630 and DUF632 domains-containing protein, strongly associated with chlorophyll content under N starvation. Loss of function of ZmNRL1 reduced nitrogen content and weakened plant growth under hydroponic and soil conditions, whereas overexpression of ZmNRL1 conferred better tolerance to N stress and elevated yields in transgenic maize and Arabidopsis. Comparative transcriptome analysis further revealed that ZmNRL1 has a broad impact on the expression of many N utilization and signaling genes. Moreover, we showed that ZmNRL1 anchored to the plasma membrane, probably through the dual lipid modifications of myristoylation and palmitoylation. Thus, we propose that ZmNRL1 is a key regulator of the adaptation response to N limitation in maize and could be a potential target for breeding high-yield maize with enhanced NUE.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3984-4002"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant sex-determining genes and the genetics of the evolution towards dioecy. 植物性别决定基因及其向雌雄异株进化的遗传学。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf224

Gabriel A B Marais, Catarina Branco, Margarida Rocheta, Mathilde Dufay, Jeanne Tonnabel

{"title":"Plant sex-determining genes and the genetics of the evolution towards dioecy.","authors":"Gabriel A B Marais, Catarina Branco, Margarida Rocheta, Mathilde Dufay, Jeanne Tonnabel","doi":"10.1093/jxb/eraf224","DOIUrl":"10.1093/jxb/eraf224","url":null,"abstract":"Thanks to significant advances in genomics and bioinformatics, research on plant sex-determining genes has made remarkable progress over the past decade. Since the discovery of the OGI-MeGI sex-determination system in persimmons in 2014, candidate sex-determining genes have been identified in a dozen flowering plant species. In this review, we examine these newly discovered genes and explore what they reveal about the genetic basis of the evolution of dioecy. While these genes are diverse, many belong to key developmental pathways previously described in other species. Two primary evolutionary routes from hermaphroditism to dioecy have been proposed: gynodioecy and monoecy. The gynodioecy pathway has been modeled with two genes and two sterility mutations, whereas recent theoretical work on the monoecy route suggests a gradual differentiation of sexual morphs involving multiple genes. Current data align with these models, particularly in the expected number of sex-determining genes. The gynodioecy pathway is typically associated with two or more genes, while the monoecy route varies, involving either one or two genes. From a molecular evolution perspective, loss-of-function mutations are frequent, but gene duplication appears to be the most common mechanism driving the emergence of new sex-determining genes. Future research should aim to characterize additional systems to gain a comprehensive view of plant sex determination and employ functional approaches to validate proposed candidate genes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3896-3911"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsGRF1 and OsGRF2 play unequal redundant roles in regulating leaf vascular bundle formation. OsGRF1和OsGRF2在调节叶片维管束形成过程中发挥着不相等的冗余作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf193

Ruihan Xu, Jianyu An, Jiali Song, Tiantian Yan, Jieru Li, Xuebin Zhao, Xi'an Shi, Yayi Meng, Chenyu Rong, Ganghua Li, Yanfeng Ding, Chengqiang Ding

{"title":"OsGRF1 and OsGRF2 play unequal redundant roles in regulating leaf vascular bundle formation.","authors":"Ruihan Xu, Jianyu An, Jiali Song, Tiantian Yan, Jieru Li, Xuebin Zhao, Xi'an Shi, Yayi Meng, Chenyu Rong, Ganghua Li, Yanfeng Ding, Chengqiang Ding","doi":"10.1093/jxb/eraf193","DOIUrl":"10.1093/jxb/eraf193","url":null,"abstract":"Leaf shape and angle are crucial components of plant architecture and substantially influence photosynthetic efficiency and crop productivity. In this study, we found that in rice (Oryza sativa) GROWTH-REGULATING FACTORS 1 and 2 (OsGRF1 and OsGRF2) play unequally redundant roles in the regulation of leaf development. The osgrf1 mutant showed increased leaf angle, and mutations in OsGRF2 further enhanced this effect, although the leaf angle of the osgrf2 mutant remained unchanged. Cell elongation on the adaxial collar side caused an increased leaf angle. rOsGRF1 rOsGRF2 material, resistant to miR396 cleavage, had reduced leaf angles. Furthermore, due to abnormal vascular bundle development, the osgrf1 osgrf2 mutant showed narrow leaves and twisted flag leaves, which may be associated with the down-regulation of the midrib-regulating gene DROOPING LEAF (DL). OsGRF1-GFP and OsGRF2-GFP were localized in leaf primordia and young leaves, which is consistent with their roles in regulating leaf development. osgrf1 osgrf2 double mutants exhibited increased sensitivity to brassinosteroids, with RNA-seq enriching for brassinosteroid-related genes. Since OsGIF3 negatively regulates leaf angle, the OsGRF1-OsGIF3 and OsGRF2-OsGIF3 complexes influence the development of the lamina joint. Haplotype analysis showed there had been artificial selection of OsGRF1 during domestication. Our findings indicate that OsGRF1/2 negatively regulates leaf angle but positively regulates grain size, which holds promise for applications in agricultural production.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4055-4070"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GmMMP9 confers resistance to soybean mosaic virus via interactions with SMV-6K1 and GmPsaN to induce a ROS response. GmMMP9通过与SMV-6K1和gmmpsan相互作用诱导ROS应答，赋予对大豆花叶病毒的抗性。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf411

Fangxue Zhou, Zhe Yu, Chen Feng, Wenmi Feng, Yonggang Zhou, Wenping Zhang, Runfa Liu, Xiangpeng Sui, Yan Jing, Haiyan Li

{"title":"GmMMP9 confers resistance to soybean mosaic virus via interactions with SMV-6K1 and GmPsaN to induce a ROS response.","authors":"Fangxue Zhou, Zhe Yu, Chen Feng, Wenmi Feng, Yonggang Zhou, Wenping Zhang, Runfa Liu, Xiangpeng Sui, Yan Jing, Haiyan Li","doi":"10.1093/jxb/eraf411","DOIUrl":"https://doi.org/10.1093/jxb/eraf411","url":null,"abstract":"Soybean mosaic virus (SMV) is a prevalent disease that significantly impacts soybean yield and quality. However, the current understanding of molecular mechanisms on soybean resistance to SMV remains limited. Here, we characterized 27 soybean matrix metalloproteinase (MMP) family members and reported the function and regulatory mechanisms of GmMMP9 in SMV resistance. Functional studies found that GmMMP9 overexpressing soybeans exhibited the enhanced resistance to SMV, while CRISPR/Cas9 mediated GmMMP9 homozygous mutants were more susceptible. DAB and trypan blue staining revealed that GmMMP9 conferred SMV resistance through the ROS production and programmed cell death triggered by hypersensitive response. Furthermore, we identified that GmMMP9 was involved in the endoplasmic reticulum (ER) stress induced by SMV via its interaction with SMV protein 6K1 (SMV-6K1). This interaction was abolished only when all four 6K1-interacting residues in GmMMP9 were mutated, suggesting that GmMMP9 inhibited SMV-6K1 function via a structural envelopment mechanism. RNA-seq analysis of GmMMP9 overexpressing, knock-out and WT plants infected with SMV revealed that several differentially expressed genes (DEGs) were involved in photosynthesis-related processes. Moreover, we found a photosystem I (PS I) reaction center subunit N (GmPsaN) interacted with GmMMP9. Silencing GmPsaN in GmMMP9 overexpressing lines, knock-out lines and WT plants led to a reduction in chlorophyll content, SOD activity, H2O2 levels and the transcription expression of ROS signaling genes, which demonstrated that this interaction affected PS I activity and thereby triggered a ROS response. Overall, our findings revealed GmMMP9 as a novel regulator of soybean resistance to SMV, and indicated the potential use for soybean molecular breeding.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of auxin-mediated gene activation in the bryophyte, Physcomitrium patens. 生长素介导的基因激活在苔藓植物中所起的作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf183

Carlisle Bascom, Daniella Tu, Shreya Repakula, Michael J Prigge, Mark Estelle

{"title":"The role of auxin-mediated gene activation in the bryophyte, Physcomitrium patens.","authors":"Carlisle Bascom, Daniella Tu, Shreya Repakula, Michael J Prigge, Mark Estelle","doi":"10.1093/jxb/eraf183","DOIUrl":"10.1093/jxb/eraf183","url":null,"abstract":"Perception and response to the hormone auxin are critical to plant growth and development. Expression of auxin-response genes is tightly regulated via known mechanisms of both activation and repression. Across the plant lineage, auxin-response gene induction is performed by AUXIN-REPSONSE FACTOR (ARF) activating transcription factors. Conversely, AUXIN/INDOLE ACETIC ACID proteins repress expression. Studies of gain-of-function constitutive repression lines and ARF loss-of-function mutants have advanced the field. Yet, there is a need for a comparative study of aberrant auxin signaling mutants to understand the developmental consequences of constitutive repression versus the absence of auxin-mediated gene induction. Using CRISPR/Cas9 gene-editing tools, we mutated each activating ARF gene in the model bryophyte, Physcomitrium patens. The resulting septuple loss-of-function mutant line (arfasept) has severe developmental phenotypes and a diminished ability to respond to exogenous auxin. However, phenotypic analysis revealed that the arfasept line is not as severely affected as the constitutive repression lines. Expression analysis of several auxin-response genes demonstrates that auxin-mediated gene induction is abolished in both arfasept and constitutive repression lines, but that basal expression levels are higher in the arfasept lines. Our results suggest that the expression of auxin-regulated genes important for developmental progression is maintained, albeit at reduced levels, in the absence of ARFs.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4043-4054"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mobile RNAs and proteins: impacts on plant growth and productivity. 移动rna和蛋白质：对植物生长和生产力的影响。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf185

Kirtikumar R Kondhare, Amey J Bhide, Anjan K Banerjee

{"title":"Mobile RNAs and proteins: impacts on plant growth and productivity.","authors":"Kirtikumar R Kondhare, Amey J Bhide, Anjan K Banerjee","doi":"10.1093/jxb/eraf185","DOIUrl":"10.1093/jxb/eraf185","url":null,"abstract":"Short- and long-distance mobile signals (mobile RNAs and proteins) are integral parts of the local and systemic communications that coordinate various physiological processes at the whole-plant level and have far-reaching impacts on plant productivity. In this review, we aim to provide a comprehensive description of the integral roles of these mobile signals in controlling phenotypic traits and plant productivity. We describe how key mobile RNAs (mRNAs, small RNAs, and long non-coding RNAs) and proteins (including RNA-binding proteins) function as vital regulators of multi-faceted aspects of phenotypic traits that ultimately govern plant productivity, such as the formation of the shoot apical meristem, leaf morphology, root architecture, flowering, ripening of fleshy fruits, tuberization, crop yield, and abiotic stress responses. We also describe recent advances in the study of macromolecular transport mechanisms, such as cyclophilin-mediated transport and extracellular vesicle-based signal delivery, as well as the identification of novel signature motifs on mobile RNAs. In addition, we consider the discovery of new mobile signals and highlight how these signals can potentially be explored with advanced biotechnological interventions, virus-induced flowering, genome-editing tools, and emerging breeding approaches (e.g. the xenia-based mobile RNA delivery system for fleshy fruits) with the aim of designing strategies for enhancing valuable phenotypic traits and improving plant productivity.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3927-3942"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abscisic acid and GIGANTEA signalling converge to regulate the recruitment of CONSTANS to the FT promoter and activate floral transition. 脱落酸和GIGANTEA信号汇聚调节CONSTANS向FT启动子的募集并激活花的转变。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf199

Alice Robustelli Test, Giorgio Perrella, Beatrice Landoni, Sara Colanero, Aldo Sutti, Paolo Korwin Krukowski, Tianyuan Xu, Elisa Vellutini, Giulia Castorina, Massimo Galbiati, Damiano Martignago, Eirini Kaiserli, Chiara Tonelli, Lucio Conti

{"title":"Abscisic acid and GIGANTEA signalling converge to regulate the recruitment of CONSTANS to the FT promoter and activate floral transition.","authors":"Alice Robustelli Test, Giorgio Perrella, Beatrice Landoni, Sara Colanero, Aldo Sutti, Paolo Korwin Krukowski, Tianyuan Xu, Elisa Vellutini, Giulia Castorina, Massimo Galbiati, Damiano Martignago, Eirini Kaiserli, Chiara Tonelli, Lucio Conti","doi":"10.1093/jxb/eraf199","DOIUrl":"10.1093/jxb/eraf199","url":null,"abstract":"Plants align flowering with optimal seasonal conditions to increase reproductive success. This process depends on modulating signalling pathways that respond to diverse environmental and hormonal inputs, thereby regulating the transition to flowering at the shoot apical meristem. In Arabidopsis, long-day photoperiods stimulate the transcription of FLOWERING LOCUS T (FT), encoding the main florigenic signal. FT activation is mediated by the transcriptional regulator CONSTANS (CO), which binds to the CO-responsive elements (COREs) located in the proximal FT promoter region. The phytohormone abscisic acid (ABA) also contributes to FT activation together with GIGANTEA (GI) to regulate drought escape. Whether CO is a target of ABA and GI actions for the regulation of FT is, however, unknown. Here, we report that ABA and its signalling components promote CO recruitment to CORE1/2, without causing reductions in the diel pattern of CO protein accumulation. ChIP-seq analyses show that ABA broadly shapes the CO DNA binding landscape, which is enriched at the promoters of genes involved in the response to abiotic stress. We also found that GI promotes CO recruitment to the CORE1/2 region, and that CO recruitment is required for the accumulation of RNA polymerase II at the transcription start site of FT. Finally, we show that GI and ABA signalling pathways are largely epistatic in the control of flowering time, suggesting their involvement in the same molecular process. Taken together, these observations suggest that varying water deficit conditions modulate CO recruitment and FT expression, thus dictating drought escape strategies in Arabidopsis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4085-4100"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homeodomain transcription factor regulation of leaf serrations in Arabidopsis thaliana. 拟南芥叶片锯齿形的同源结构域转录因子调控。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf174

Eleanor E Imlay, Hyung-Woo Jeon, Mary E Byrne

{"title":"Homeodomain transcription factor regulation of leaf serrations in Arabidopsis thaliana.","authors":"Eleanor E Imlay, Hyung-Woo Jeon, Mary E Byrne","doi":"10.1093/jxb/eraf174","DOIUrl":"10.1093/jxb/eraf174","url":null,"abstract":"Arabidopsis thaliana has simple spatulate to obovate shaped leaves, with subtle serrations along the margin. The BEL1-like homeodomain transcription factors (BLH) SAWTOOTH1 and SAWTOOTH2, and the class II KNOTTED1-like homeobox (KNOXII) KNAT3 are suppressors of serration outgrowths. Double saw1 saw2 mutants have enhanced serrations and knat3 single mutants have a subtle increase in serrations relative to the wild type. However, the relationship between these homeodomain proteins is unclear. Here we show that SAW1, SAW2, and KNAT3 have overlapping expression patterns on the adaxial side of the leaf and saw1 saw2 knat3 triple mutants show greatly enhanced leaf serration relative to the saw1 saw2 double mutant and knat3 single mutant. KNAT3 expressed in the adaxial leaf from the endogenous promoter suppresses the saw1 saw2 knat3 phenotype, whereas ectopic expression throughout the leaf or in the abaxial domain enhances the triple mutant serration phenotype. This ectopic KNAT3 activity can be modified by ectopic expression of SAW1. These results demonstrate that the adaxial-abaxial domain specificity of BLH and KNOXII homeodomain proteins has consequences for serration geometry.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4014-4026"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruption of the karrikin receptor DWARF 14 LIKE (D14L) gene leads to distinct effects on root and shoot growth, and reprogramming of central metabolism in rice. Karrikin受体DWARF 14 LIKE （D14L）基因的破坏对水稻根冠生长和中心代谢重编程的影响

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf201

Mohamed A Salem, Muhammad Jamil, Jian You Wang, Lamis Berqdar, Kit Xi Liew, Anggra Paramita, Abdugaffor Ablazov, Aparna Balakrishna, Salim Al-Babili

{"title":"Disruption of the karrikin receptor DWARF 14 LIKE (D14L) gene leads to distinct effects on root and shoot growth, and reprogramming of central metabolism in rice.","authors":"Mohamed A Salem, Muhammad Jamil, Jian You Wang, Lamis Berqdar, Kit Xi Liew, Anggra Paramita, Abdugaffor Ablazov, Aparna Balakrishna, Salim Al-Babili","doi":"10.1093/jxb/eraf201","DOIUrl":"10.1093/jxb/eraf201","url":null,"abstract":"The rice (Oryza sativa L.) α/β-hydrolase D14 LIKE (D14L), a paralog of the strigolactone receptor D14, is essential for the establishment of arbuscular mycorrhizal (AM) symbiosis and responses to karrikins, smoke-derived compounds that regulate several developmental processes. It is thought that D14L is the receptor for an as yet unidentified endogenous growth regulator. Herein, we determined the effects of disrupting D14L on rice growth and metabolism. Our results revealed that D14L loss of function altered rice architecture, leading to a significant increase in root growth and mesocotyl elongation, while hindering shoot growth, and a notable decrease in the number of tillers, especially under phosphate-limiting conditions. Furthermore, d14l mutants exhibited significant reduction in total grain yield. Metabolomics analysis revealed a notable shift of key metabolites, such as carbohydrates and amino acids involved in energy production and growth, from shoots to roots. This redistribution is likely to reflect an adaptive strategy to enhance nutrient acquisition and increase root biomass, albeit at the cost of shoot growth and productivity. Our findings highlight the pivotal role of D14L in regulating the root-to-shoot growth ratio and in coordinating the metabolism and allocation of resources across the rice plant.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4114-4128"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448878/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0