Journal of Experimental Botany最新文献_第8页

Investigating how reproductive traits in rice respond to abiotic stress. 研究水稻生殖性状对非生物胁迫的响应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf031

Claudia Maria Liberatore, Marco Biancucci, Ignacio Ezquer, Veronica Gregis, Maurizio Di Marzo

引用次数: 0

The Arabidopsis eATP receptor DORN1 and CNGC19 calcium channel act in tandem to regulate plant defense upon Spodoptera litura herbivory. 拟南芥eATP受体、DORN1和CNGC19通道共同调控植物对斜纹夜蛾的防御。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf025

Pritha Kundu, Misha Kumari, Mukesh Kumar Meena, Shruti Mishra, Jyothilakshmi Vadassery

{"title":"The Arabidopsis eATP receptor DORN1 and CNGC19 calcium channel act in tandem to regulate plant defense upon Spodoptera litura herbivory.","authors":"Pritha Kundu, Misha Kumari, Mukesh Kumar Meena, Shruti Mishra, Jyothilakshmi Vadassery","doi":"10.1093/jxb/eraf025","DOIUrl":"10.1093/jxb/eraf025","url":null,"abstract":"Plants deploy cellular calcium (Ca2+) elevation in environmental stress signaling. Extracellular ATP (eATP) is released into the extracellular matrix when cells are wounded. DOES NOT RESPOND TO NUCLEOTIDES 1 (DORN1), a key legume-type lectin receptor, senses and binds eATP and activates Ca2+ signaling. No evidence directly links calcium-mediated eATP signaling to resistance against insect herbivores. Here, we report up-regulation of DORN1 transcripts in response to wounding and Spodoptera litura feeding in Arabidopsis. Loss of function of DORN1 resulted in increased S. litura feeding compared with the wild type. Plant immunity was compromised in dorn1 mutants as they showed reduced S. litura oral secretion-mediated Ca2+ elevation, jasmonic acid accumulation, and expression of jasmonate responsive genes. The herbivory-induced calcium channel, CYCLIC NUCLEOTIDE GATED CHANNEL 19 (CNGC19), co-expresses with DORN1. We found that eATP-induced Ca2+ elevation requires functional CNGC19. Loss of function of DORN1 and CNGC19 substantially increased the susceptibility to S. litura, mediated by reduced accumulation of jasmonates. We also demonstrated a plausible interaction of CNGC19 with DORN1. Our results indicate damage-released eATP and its receptor DORN1 in herbivory-induced defense signaling. DORN1 together with the Ca2+ channel CNGC19 generate the eATP-induced Ca2+ elevation, leading to the activation of immune signaling.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2264-2277"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of m6A modification and transcript quantity on mRNA composition in plant stress granules under hypoxia. m6A修饰及转录量对植物胁迫颗粒中mRNA组成的影响

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf046

Dawid Jakub Kubiak, Michał Wojciech Szcześniak, Karolina Ostrowska, Dawid Bielewicz, Susheel Sagar Bhat, Katarzyna Niedojadło, Zofia Szweykowska-Kulińska, Artur Jarmołowski, Rupert George Fray, Janusz Niedojadło

{"title":"Impact of m6A modification and transcript quantity on mRNA composition in plant stress granules under hypoxia.","authors":"Dawid Jakub Kubiak, Michał Wojciech Szcześniak, Karolina Ostrowska, Dawid Bielewicz, Susheel Sagar Bhat, Katarzyna Niedojadło, Zofia Szweykowska-Kulińska, Artur Jarmołowski, Rupert George Fray, Janusz Niedojadło","doi":"10.1093/jxb/eraf046","DOIUrl":"10.1093/jxb/eraf046","url":null,"abstract":"Stress granules (SGs) are cytoplasmic structures that appear in response to unfavorable environmental conditions. The mechanisms governing the accumulation of transcripts in SGs are only partially understood, and despite the recognized role of N6-methyladenosine (m6A) in plant transcriptome regulation, its impact on SG composition and assembly remains unknown. In Lupinus angustifolius, SGs display a distinctive bi-zonal structure comprising of a ring and a central area with differences in their ultrastructure and composition. Following a transcriptome analysis, specific mRNAs were chosen to investigate their localization within SGs and to assess m6A levels. Transcripts of hypoxia-responsive genes (ADH1 and HUP7) showed significantly lower levels of m6A compared to housekeeping genes, but only ADH1 was absent in SGs. HUP7 mRNA, characterized by a low quantity of m6A, was present both in the SGs and the cytoplasm, probably due to an extremely high expression level. The m6A modification was observed only during the assembly of SGs. In mutants of Arabidopsis with reduced levels of m6A, ECT2 (a reader of m6A) was not observed in SGs, and poly(A) RNA levels and the number of SGs were reduced. Our findings thus demonstrate a limited impact of m6A modification on SG assembly; however, the interplay between m6A modification and the overall transcript quantity in the cytoplasm appears to play a regulatory role in mRNA partitioning and assembly of SGs.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2338-2355"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nicotiana benthamiana VASCULAR-RELATED NAC-DOMAIN7-2 (NbVND7-2) has a role in xylem formation during interfamily grafting. benthamiana VASCULAR-RELATED NAC-DOMAIN7-2 （NbVND7-2）在家族间嫁接木质部形成中起作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf074

Chaokun Huang, Koichi Toyokura, Ei-Ichi Murakami, Aiko Ishiwata, Ken-Ichi Kurotani, Michitaka Notaguchi

{"title":"Nicotiana benthamiana VASCULAR-RELATED NAC-DOMAIN7-2 (NbVND7-2) has a role in xylem formation during interfamily grafting.","authors":"Chaokun Huang, Koichi Toyokura, Ei-Ichi Murakami, Aiko Ishiwata, Ken-Ichi Kurotani, Michitaka Notaguchi","doi":"10.1093/jxb/eraf074","DOIUrl":"10.1093/jxb/eraf074","url":null,"abstract":"VASCULAR-RELATED NAC-DOMAIN7 (VND7) is a transcription factor gene that plays a critical role in xylem differentiation. The ectopic expression of VND7 induces the formation of secondary cell walls with spiral patterns in multiple plant cell types. In the present study, we identified four homologs of VND7 in Nicotiana benthamiana and assigned them the names NbVND7-1 to NbVND7-4. In particular, NbVND7-1 and NbVND7-2 were highly expressed during N. benthamiana and Arabidopsis thaliana (Nb/At) interfamily grafting. Analysis of the promoter β-glucuronidase (GUS) reporter lines of NbVND7 genes elucidated the expression of NbVND7-1 and NbVND7-2 in xylem tissues of intact and grafted plants, and those of NbVND7-3 and NbVND7-4 in internal phloem tissues. Gene network analysis revealed the downstream genes of each NbVND7 homolog and highlighted the association of NbVND7-1 and NbVND7-2 with xylem formation. A β-estradiol-inducible system for NbVND7-2 demonstrated that NbVND7-2 promotes ectopic xylem vessel differentiation in N. benthamiana seedlings and in the stem tissues at graft junctions. Induction of NbVND7-2 at graft junctions enhanced ectopic xylem formation in the callus tissues proliferated at the graft boundary, accelerated the initiation of water transport from stock to scion, and enhanced scion stem growth after grafting. This study reveals a role for NbVND7 genes in xylem formation that can enhance Nicotiana interfamily grafting.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2207-2221"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dancing molecules: group A bZIPs and PEBPs at the heart of plant development and stress responses. 跳舞分子：A组bzip和pebp在植物发育和胁迫反应中的核心作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf034

Damiano Martignago, Vítor da Silveira Falavigna, George Coupland, Lucio Conti

{"title":"Dancing molecules: group A bZIPs and PEBPs at the heart of plant development and stress responses.","authors":"Damiano Martignago, Vítor da Silveira Falavigna, George Coupland, Lucio Conti","doi":"10.1093/jxb/eraf034","DOIUrl":"10.1093/jxb/eraf034","url":null,"abstract":"Group A basic leucine zipper (bZIP) transcription factors play critical roles in abscisic acid (ABA) signaling and plant development. In Arabidopsis thaliana, these factors are defined by a highly conserved core bZIP domain, and four conserved domains throughout their length: three at the N-terminus (C1-C3) and a phosphorylatable C-terminal SAP motif located at the C4 domain. Initially, members such as ABI5 and ABFs were studied for their roles in ABA signaling during seed germination or stress responses. Later, a sub-clade of group A bZIPs, including FD, was found to play important roles in floral induction by interacting with the florigen FLOWERING LOCUS T (FT) at the shoot apical meristem. Recent research has expanded our understanding of these transcription factors by identifying intriguing parallels between those involved in ABA signaling and those promoting floral induction, and revealing dynamic interactions with FT and other phosphatidylethanolamine-binding proteins (PEBPs) such as TERMINAL FLOWER 1. Studies in crop plants and non-model species demonstrate broader roles, functions, and molecular targets of group A bZIPs. This review highlights common features of group A bZIPs and their post-translational regulation in enabling the activation of gene regulatory networks with important functions in plant development and stress responses.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2081-2095"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is there an oilseed rape ideotype that can efficiently withstand sulfur deficiency? 有没有一种油菜能有效地抵御硫缺乏？

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf133

Dimitris L Bouranis, Styliani N Chorianopoulou

引用次数: 0

From Biosynthesis to Signaling: Unveiling the Multifaceted Roles of Phytosulfokine Peptide in Plants. 从生物合成到信号传导：揭示植物磺胺肽在植物中的多重作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-23 DOI: 10.1093/jxb/eraf230

Changqi Wu, Raheel Munir, Fangfang Li, Pingfang Li, Yi Cai, Kai Shi

引用次数: 0

Salicylic acid-induced stomatal closure differs in vascular plants of various lineages. 水杨酸诱导的气孔关闭在不同系维管植物中是不同的。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf227

Xiayi Zhang, Guoliang Chen, Xudong Liu, Minhui Bi, Shihua Qi, Yuanyuan Zeng, Yanru Li, Yaxian Du, Xueqian Tian, Ruiyun He, Xiangwen Fang

{"title":"Salicylic acid-induced stomatal closure differs in vascular plants of various lineages.","authors":"Xiayi Zhang, Guoliang Chen, Xudong Liu, Minhui Bi, Shihua Qi, Yuanyuan Zeng, Yanru Li, Yaxian Du, Xueqian Tian, Ruiyun He, Xiangwen Fang","doi":"10.1093/jxb/eraf227","DOIUrl":"https://doi.org/10.1093/jxb/eraf227","url":null,"abstract":"Salicylic acid (SA)-triggering stomatal closure to prevent pathogen invasion has been widely recognized in angiosperms. However, whether SA-induced stomal closure differs across vascular plants of various lineages remains unclear. In this study, the stomatal responses of 28 species, including 13 angiosperms, 6 gymnosperms, 8 ferns, and 1 lycophyte, to exogenous SA were evaluated, and the stomatal closure signaling pathways of 6 representative species were subsequently examined. SA-induced stomatal closure was observed in seed plants but not in the ferns or lycophyte. Additionally, SA induced an increase in ROS in the guard cells of these 6 different vascular plants, but NO accumulation in and K+ efflux from guard cells were detected in seed plants but not in the 2 ferns or 1 lycophyte investigated. The application of SA induced large vacuolar compartments in the guard cells of the seed plants to disaggregate, whereas no such changes were observed in the guard cells of the ferns or lycophyte. The differences in the responses of the seed plant, fern, and lycophyte stomata to SA observed in this study were due mainly to the partial absence of the SA signaling pathway in the ferns and lycophyte.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interference with phytohormone signaling by whiteflies differentially affects a plant's attractiveness to a larval and an egg parasitoid of the cabbage white butterfly. 白蝇对植物激素信号的干扰不同程度地影响了植物对白菜白蝴蝶幼虫和卵类寄生虫的吸引力。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf208

Yu-Mei Dong, Ya-Li Sang, Shu-Zhen Wang, Ted C J Turlings, Ye-Hua Li, Da-Wei Xue, Peng-Jun Zhang

{"title":"Interference with phytohormone signaling by whiteflies differentially affects a plant's attractiveness to a larval and an egg parasitoid of the cabbage white butterfly.","authors":"Yu-Mei Dong, Ya-Li Sang, Shu-Zhen Wang, Ted C J Turlings, Ye-Hua Li, Da-Wei Xue, Peng-Jun Zhang","doi":"10.1093/jxb/eraf208","DOIUrl":"https://doi.org/10.1093/jxb/eraf208","url":null,"abstract":"In response to egg deposition or feeding by insect herbivores plants release specific blends of volatiles that attract natural enemies of the herbivores. In nature, plants are often simultaneously attacked by multiple herbivores that may induce different signaling pathways, thus affecting the volatile blends and interfering with the attraction of the natural enemies. The mechanisms underlying such interference remain largely unknown. Here, we show that co-infestation of Arabidopsis thaliana plants with the whitefly Bemisia tabaci reduces the volatile emissions induced by Pieris rapae caterpillars, resulting in reduced attraction of the larval parasitoid Cotesia rubecula. Hormone and gene expression analyses, followed by assays with various Arabidopsis mutants revealed that this interference by B. tabaci is the result of antagonistic crosstalk between jasmonic acid (JA) and salicylic acid (SA), involving the transcription factor NPR1 but not WRKY70. In contrast, B. tabaci co-infestation had no impact on the attraction of an egg parasitoid to P. rapae egg-induced volatiles. These differential effects of the whitefly on the behavior of the two parasitoids were confirmed in greenhouse assays. This study provides new insight into the molecular mechanisms underlying the recruitment of different parasitoids by plants and could help in developing effective biocontrol strategies.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small RNA mobility and plant virus diseases. 小RNA迁移与植物病毒病。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf226

Laura Elvira-Gonzalez, Todd Blevins, Manfred Heinlein

引用次数: 0