Journal of Experimental Botany最新文献_第8页

Comparison of linkage and association mapping in MAGIC lines identifies AtMTP3 as a new gene controlling natural variation in leaf zinc concentration in Arabidopsis. 通过MAGIC系连锁定位和关联定位的比较，鉴定出AtMTP3是控制拟南芥叶片锌浓度自然变异的新基因。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf142

Felipe Klein Ricachenevsky, Ana Carolina A L Campos, Paloma Koprovski Menguer, Fernando Mateus Michelon Betin, Jaime Tovar, William F A van Dijk, Mary Lou Guerinot, David E Salt, Paula X Kover

{"title":"Comparison of linkage and association mapping in MAGIC lines identifies AtMTP3 as a new gene controlling natural variation in leaf zinc concentration in Arabidopsis.","authors":"Felipe Klein Ricachenevsky, Ana Carolina A L Campos, Paloma Koprovski Menguer, Fernando Mateus Michelon Betin, Jaime Tovar, William F A van Dijk, Mary Lou Guerinot, David E Salt, Paula X Kover","doi":"10.1093/jxb/eraf142","DOIUrl":"10.1093/jxb/eraf142","url":null,"abstract":"The Arabidopsis thaliana MAGIC lines are the result of extensive recombination among 19 accessions, which allows a direct comparison of association and linkage mapping using the same population. We used both approaches to map the genetic basis of natural variation in the leaf ionome of A. thaliana. We found 57 quantitative trait loci (QTL) and 10 significant associations, eight of which co-locate with QTL analysis. This suggests that the genome-wide association has a low rate of false positives in these MAGIC lines, but an overall lower power to identify potential genetic factors explaining natural variation. We replicated several loci previously identified by linkage or association studies and identified new candidate genes. We demonstrated the success of this approach by validating AtMTP3 (a vacuolar zinc and cobalt transporter) as the cause of natural variation in zinc leaf concentration. We showed that Kn-0, one of the MAGIC lines founder accessions, carries a rare AtMTP3 allele that results in increased zinc concentration in leaves. Yeast mutant complementation suggests that Kn-0 AtMTP3 encodes a hypofunctional protein compared with Col-0. Our work demonstrates that natural variation in Zn leaf concentration is linked to vacuolar transport and Zn sequestration in roots, opening up new avenues to manipulate Zn concentration in plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3972-3983"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixing varieties mitigates early root competition in wheat under water and nutrient limitation. 混合品种减轻了水分和养分限制下小麦早期根系竞争。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf163

Germain Montazeaud, Pierre Roumet, Mickaël Lamboeuf, Christian Jeudy, Martin Ecarnot, Lise Malicet-Chebbah, Christophe Salon, Hélène Fréville

{"title":"Mixing varieties mitigates early root competition in wheat under water and nutrient limitation.","authors":"Germain Montazeaud, Pierre Roumet, Mickaël Lamboeuf, Christian Jeudy, Martin Ecarnot, Lise Malicet-Chebbah, Christophe Salon, Hélène Fréville","doi":"10.1093/jxb/eraf163","DOIUrl":"10.1093/jxb/eraf163","url":null,"abstract":"Competition between plants can lead to a tragedy of the commons (TOC), where excessive investment in resource-harvesting organs reduces collective performance. Mixing crop varieties could resolve such TOCs through niche complementarity-if varieties differ in resource use-or selection effects, where competitive varieties benefit from weaker neighbours. While most studies on varietal mixtures focus on above-ground traits, below-ground interactions remain poorly understood. We grew 36 durum wheat (Triticum turgidum ssp. durum) varieties in pure stands and 54 binary mixtures using a high-throughput root phenotyping platform, under both non-limiting (R+) and limiting (R-) water and nutrient conditions, to assess early-stage root competition. In R-, mixtures produced less biomass than expected based on pure stands, largely due to a negative complementarity effect. This was mostly explained by the average projected root area of the two varieties. Rather than indicating a negative interaction, the effect reflected a relaxation of competition: varieties with larger root systems benefited from having weaker competitors, disengaging from the arms race for biomass accumulation. These findings suggest that root area is a promising breeding target for mitigating intra-specific competition and a critical trait for assembling optimal varietal mixtures.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4171-4184"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of the formation of acquired drought tolerance in wheat: insights from combining high-throughput phenotyping and genome-wide association study. 小麦获得性耐旱性形成的机制：高通量表型和全基因组关联研究的结合。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf124

Qing Li, Shuming Fan, Junfeng Cao, Zhuangzhuang Sun, Chuan Zhong, Haijiang Min, Shaowei Liang, Xiao Wang, Qin Zhou, Jian Cai, Yingxin Zhong, Mei Huang, Dong Jiang

{"title":"Mechanisms of the formation of acquired drought tolerance in wheat: insights from combining high-throughput phenotyping and genome-wide association study.","authors":"Qing Li, Shuming Fan, Junfeng Cao, Zhuangzhuang Sun, Chuan Zhong, Haijiang Min, Shaowei Liang, Xiao Wang, Qin Zhou, Jian Cai, Yingxin Zhong, Mei Huang, Dong Jiang","doi":"10.1093/jxb/eraf124","DOIUrl":"10.1093/jxb/eraf124","url":null,"abstract":"Drought priming represents a potential strategy to bolster wheat yields in the face of recurring droughts, and there is a need to identify responsive cultivars and decipher the underlying mechanisms of priming. Here, the responses of 157 wheat cultivars to drought-priming were phenotyped using a high-throughput phenotyping (HTP) platform across two growing seasons, and a drought priming index (DPI) was devised to assess the priming sensitivity for each cultivar. A DPI comprehensive score (DPICS) was derived from 13 sensitive traits identified by principal component analysis, and significant variations in this score led to the classification of the cultivars into two distinct groups, one sensitive to drought priming and one not. The sensitive group contained 58 cultivars that had higher DPI values for traits including yield components, harvest index, post-anthesis assimilation, photochemical efficiency, canopy coverage, and normalized difference vegetation index, and lower DPI values for traits including remobilization of dry matter stored pre-anthesis, non-photochemical quenching, plant senescence reflectance index, and canopy temperature. A genome-wide association study (GWAS) based on the DPI identified 499 significant markers related to drought priming using a commercially Wheat660 SNP array. Notably, one marker situated on chromosome 5B consistently appeared in both the growing seasons that were studied. This marker resides within a 261.2 kb genomic block containing seven genes, including the candidate gene TraesCS5B03G1259700, which exhibited distinct transcriptional memory related to drought priming. Our results suggest that integrating HTP and GWAS has great potential for deciphering the genetic basis of acquired drought tolerance induced by priming and could facilitate the breeding of improved wheat varieties that can respond to recurring drought events.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4154-4170"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Transcriptional regulation of development by SMAX1-LIKE proteins - targets of strigolactone and karrikin/KAI2 ligand signaling. 修正：SMAX1-LIKE蛋白对发育的转录调控-独角麦内酯和karrikin/KAI2配体信号传导的靶点。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf242

引用次数: 0

The role of α-globulin accumulation in seed storage protein reprogramming and grain quality in rice. α-球蛋白积累在水稻种子贮藏、蛋白质重编程和籽粒品质中的作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf410

Feifei Xu, Yanni Zhang, Xinyu Li, Jianming Pan, Meng Li, Jing Yu, Lin Zhang, Yong-Jin Park, Jinsong Bao

{"title":"The role of α-globulin accumulation in seed storage protein reprogramming and grain quality in rice.","authors":"Feifei Xu, Yanni Zhang, Xinyu Li, Jianming Pan, Meng Li, Jing Yu, Lin Zhang, Yong-Jin Park, Jinsong Bao","doi":"10.1093/jxb/eraf410","DOIUrl":"https://doi.org/10.1093/jxb/eraf410","url":null,"abstract":"The 26-kDa α-globulin, a minor component of rice seed storage proteins (SSPs), is encoded by the single-copy gene α-globulin 1 (Glb1), which is specifically expressed in the endosperm. However, the specific effects of altered α-globulin content on SSP composition and grain quality remains poorly understood. In this study, we demonstrated that modulation of α-globulin levels significantly enhances both total protein content and essential amino acid lysine accumulation in Glb1 knockout mutants and overexpression lines. Notably, specific alterations in SSP composition were observed exclusively in Glb1 mutants, with no such changes detected in overexpression lines. Glb1 knockout mutant produced abnormal protein bodies, including small-sized protein body I (PBI) and center-cracked PBII. Furthermore, increased proglutelin accumulation in Glb1 mutants triggered endoplasmic reticulum (ER) stress, resulting in a higher chalky grain rate. However, the altered α-globulin content had minimal effects on grain weight and starch properties compared to the wild type. This study elucidates the role of α-globulin accumulation in SSP reprogramming and provides a potential strategy for enhancing rice endosperm lysine content through the regulation of Glb1 biosynthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epitranscriptomic Control of Plant 18S rRNA m6A Modification for mRNA Translation. 植物18S rRNA m6A修饰mRNA翻译的表转录组学控制。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf416

Chao Zheng, Jian-Ping Chen, Xiao-Wei Wang, Ping Li

引用次数: 0

Meeting Liquid Biofuel and Bioproduct Goals: Biotechnological Design of the Intermediate Oilseeds Pennycress and Camelina, and Beyond. 满足液体生物燃料和生物产品的目标：中间油籽pennyress和Camelina等的生物技术设计。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf415

Barsanti Gautam, Hyojin Kim, Chaofeng Wang, Kiyoul Park, Edgar B Cahoon, John C Sedbrook

{"title":"Meeting Liquid Biofuel and Bioproduct Goals: Biotechnological Design of the Intermediate Oilseeds Pennycress and Camelina, and Beyond.","authors":"Barsanti Gautam, Hyojin Kim, Chaofeng Wang, Kiyoul Park, Edgar B Cahoon, John C Sedbrook","doi":"10.1093/jxb/eraf415","DOIUrl":"https://doi.org/10.1093/jxb/eraf415","url":null,"abstract":"The European Union (EU) and United States (U.S.) have set ambitious goals to produce biofuels as part of broader decarbonization and energy security initiatives. One of the more feasible routes to liquid biofuels production is the conversion of seed oils (triacylglycerols; TAG) to renewable diesel, biodiesel, and sustainable aviation fuel (SAF) using the Hydrotreated Ester and Fatty Acids (HEFA) process. Camelina and pennycress are attractive oilseed feedstocks in that they can be grown in the offseason as intermediate crops on tens of millions of hectares of farmland annually, providing ecosystem benefits and not competing with established food crops. Considerably more TAG could be produced by engineering vegetative portions of crops such as sorghum and miscanthus to accumulate economic amounts. This review highlights recent advances in developing pennycress and camelina as intermediate oilseed crops not only for biofuels production but for making higher value oils such as those enriched in astaxanthin, vitamin E, and medium-chain fatty acids. Given the magnitude of renewable liquid fuel demands, we also describe how advances in vegetative oil production in biomass crops can complement intermediate oilseed cropping systems to meet biofuel and bioproduct targets.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative assessment of hormogonia induction in Nostoc punctiforme by a fluorescent reporter strain. 荧光报告菌株诱导点状念珠菌的定量评价。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf197

Anna Neubauer, Macarena Iniesta-Pallarés, Consolación Álvarez, Aurélien Bailly, Péter Szövényi, Vicente Mariscal

{"title":"Quantitative assessment of hormogonia induction in Nostoc punctiforme by a fluorescent reporter strain.","authors":"Anna Neubauer, Macarena Iniesta-Pallarés, Consolación Álvarez, Aurélien Bailly, Péter Szövényi, Vicente Mariscal","doi":"10.1093/jxb/eraf197","DOIUrl":"10.1093/jxb/eraf197","url":null,"abstract":"While symbiotic plant-cyanobacteria interactions hold significant potential for revolutionizing agricultural practices by reducing the application of artificial nitrogen fertilizers, the genetic underpinnings of the symbiotic interaction between the plant host and the cyanobiont remain poorly understood. In particular, the molecular mechanisms through which host plants induce the formation of motile cyanobacterial filaments (hormogonia), essential for colonization and initiation of symbiosis, are not well characterized. In this study, we present a novel yet objective method for quantifying hormogonia induction, addressing limitations of traditional qualitative approaches. We have developed a reporter strain of Nostoc punctiforme PCC 73102 capable of quantifying hormogonia induction in response to diverse biotic and abiotic stimuli. This reporter strain, generated via triparental mating conjugation transformation, contains the promoter sequence of prepilin pilA fused to a green fluorescent protein (GFP) and enables quantitative and high-throughput monitoring of hormogonia induction using a microplate reader. Our innovative approach, employing a cyanobacterial hormogonia reporter strain, allows high-throughput screening of the hormogonia-inducing effect of a wide array of environmental and plant signals. This method is expected to greatly advance our understanding of the genetic determinants underpinning plant-cyanobacteria symbioses.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"3943-3953"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative genetics of photosynthetic trait variation in maize. 玉米光合性状变异的数量遗传学研究。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf198

Waqar Ali, Marcin Grzybowski, J Vladimir Torres-Rodríguez, Fangyi Li, Nikee Shrestha, Ramesh Kanna Mathivanan, Gabriel de Bernardeaux, Khang Hoang, Ravi V Mural, Rebecca L Roston, James C Schnable, Seema Sahay

{"title":"Quantitative genetics of photosynthetic trait variation in maize.","authors":"Waqar Ali, Marcin Grzybowski, J Vladimir Torres-Rodríguez, Fangyi Li, Nikee Shrestha, Ramesh Kanna Mathivanan, Gabriel de Bernardeaux, Khang Hoang, Ravi V Mural, Rebecca L Roston, James C Schnable, Seema Sahay","doi":"10.1093/jxb/eraf198","DOIUrl":"10.1093/jxb/eraf198","url":null,"abstract":"Natural genetic variation in photosynthesis-related traits can help both to identify genes involved in regulating photosynthetic processes and to develop crops with improved productivity and photosynthetic efficiency. However, rapidly fluctuating environmental parameters create challenges for measuring photosynthetic parameters in large populations under field conditions. We measured chlorophyll fluorescence and absorbance-based photosynthetic traits in a maize diversity panel in the field using an experimental design that allowed us to estimate and control multiple confounding factors. Controlling the impact of day of measurement and light intensity as well as patterns of two-dimensional spatial variation in the field increased heritability for 11 out of 14 traits measured. We were able to identify high-confidence genome-wide association study (GWAS) signals associated with variation in four spatially corrected traits (the quantum yield of PSII, non-photochemical quenching, redox state of QA, and relative chlorophyll content). Insertion alleles for Arabidopsis orthologs of three candidate genes exhibited phenotypes consistent with our GWAS results. Collectively these results illustrate the potential of applying best practices from quantitative genetics research to address outstanding questions in plant physiology and to understand natural variation in photosynthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4141-4153"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Declining growth and changes in biomass allocation with warming in rainforest trees from temperate to tropical climates. 从温带到热带气候的雨林树木生长下降和生物量分配的变化。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-17 DOI: 10.1093/jxb/eraf186

Zineb Choury, Yanisa Olaranont, Elise Dando, Belinda E Medlyn, Mark G Tjoelker, Kristine Y Crous

{"title":"Declining growth and changes in biomass allocation with warming in rainforest trees from temperate to tropical climates.","authors":"Zineb Choury, Yanisa Olaranont, Elise Dando, Belinda E Medlyn, Mark G Tjoelker, Kristine Y Crous","doi":"10.1093/jxb/eraf186","DOIUrl":"10.1093/jxb/eraf186","url":null,"abstract":"Australian rainforests occur from temperate to tropical latitudes, but how climate warming will affect tree growth along this climate gradient remains poorly understood. We examined how changes in biomass allocation, leaf area, and photosynthetic capacity were linked to the capacity to maintain growth rates with +3.5, +7 and +10.5 °C warming in seedlings of 12 tropical, subtropical, and temperate rainforest tree species. Temperate species maintained or increased final biomass (+14%), leaf area, and leaf area ratio (the ratio of leaf area to plant dry mass) with warming, along with similar or increased leaf mass fraction (+14%). Subtropical species increased biomass with +3.5 °C (+28%) and +7 °C (+17%) warming but were negatively impacted with >10 °C warming (-31%). Tropical species reduced leaf area, leaf area ratio, photosynthetic capacity, and leaf nitrogen, along with 14% increased root allocation in response to warming, resulting in large biomass reductions with +3.5 °C (-20%) and +7 °C (-53%). Tropical species were more susceptible to climate warming with reduced photosynthetic capacity and reduced biomass, which can lead to a reduced carbon sink in the future, especially in late-successional tree species, which are the most abundant with a large role in carbon assimilation of tropical rainforests.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4185-4200"},"PeriodicalIF":5.7,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12448803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0