Journal of Experimental Botany最新文献_第8页

The PEBP genes FLOWERING LOCUS T and TERMINAL FLOWER 1 modulate seed dormancy and size. PEBP基因开花位点T和顶花1调控种子的休眠和大小。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae466

Judit Nadal Bigas, Martijn Fiers, Froukje van der Wal, Leo A J Willems, Viola Willemsen, Harm Nijveen, Gerco C Angenent, Richard G H Immink

{"title":"The PEBP genes FLOWERING LOCUS T and TERMINAL FLOWER 1 modulate seed dormancy and size.","authors":"Judit Nadal Bigas, Martijn Fiers, Froukje van der Wal, Leo A J Willems, Viola Willemsen, Harm Nijveen, Gerco C Angenent, Richard G H Immink","doi":"10.1093/jxb/erae466","DOIUrl":"10.1093/jxb/erae466","url":null,"abstract":"The phosphatidylethanolamine-binding protein (PEBP) family members FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) are major regulators of plant reproduction. In Arabidopsis, the FT/TFL1 balance defines the timing of floral transition and the determination of inflorescence meristem identity. However, emerging studies have elucidated a plethora of previously unknown functions for these genes in various physiological processes. Here, we characterized potential roles in seed size and dormancy of FT and TFL1 in Arabidopsis thaliana using CRISPR mutants and reporter analysis. Our findings unveiled a role for TFL1 in seed dormancy while confirming the role of FT in regulating this trait. We showed that the interplay between these two genes in seed dormancy is antagonistic, mirroring their roles in flowering time and inflorescence architecture. Analysis of reporter lines demonstrated that FT and TFL1 are partly co-expressed in seeds. Finally, we showed that total seed yield is affected in these mutants. Together, our results highlight the versatility of these two genes beyond their canonical functions. The impact of FT and TFL1 on seed characteristics emphasizes the significance of approaching gene studies from various perspectives, enabling the identification of multifaceted molecular factors that could play a major role in shaping the future of agriculture.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1049-1067"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

More than a passive barrier: algal cell walls play an active role in determining cell shape, cell size, and organelle morphology.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae411

Natalie Hoffmann

引用次数: 0

Root sweet root: how date palm uses osmotic adjustment to thrive in arid climates.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/eraf022

Virginia Hernandez-Santana, Javier Pichaco

引用次数: 0

Recycling of purine nucleotides in legumes: functional specialization of enzyme isoforms in adenine salvage, cytokinin homeostasis, and nodulation control.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/eraf008

Manuel Becana

引用次数: 0

Unveiling the secrets of lotus seed longevity: insights into adaptive strategies for extended storage. 揭开莲子长寿的秘密：深入了解延长储存的适应性策略。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae432

Heng Sun, Jia Xin, Abid Ullah, Heyun Song, Lin Chen, Dong Yang, Xianbao Deng, Juan Liu, Ray Ming, Minghua Zhang, Hui Yang, Gangqiang Dong, Mei Yang

{"title":"Unveiling the secrets of lotus seed longevity: insights into adaptive strategies for extended storage.","authors":"Heng Sun, Jia Xin, Abid Ullah, Heyun Song, Lin Chen, Dong Yang, Xianbao Deng, Juan Liu, Ray Ming, Minghua Zhang, Hui Yang, Gangqiang Dong, Mei Yang","doi":"10.1093/jxb/erae432","DOIUrl":"10.1093/jxb/erae432","url":null,"abstract":"Seed longevity is crucial for long-term storage, but prolonged unfavorable conditions can lead to loss of viability. This study integrated theoretical and experimental techniques to elucidate the inherent mechanisms underlying the unique ability of lotus seeds to maintain stable viability over many years. Transcriptome analysis and microscopy revealed a sturdy structure of the lotus seed pericarp, which predominantly expressed cellulose synthase genes involved in cell wall biogenesis. The cotyledon serves as a nutrient source for seeds during long-term storage. Additionally, the inactivation of chlorophyll degradation pathways may allow for the retention of chlorophyll in the lotus seed plumule, potentially enhancing the environmental adaptability of lotus seedlings. Reduced abundance of transcripts corresponding to heat shock protein genes could impact protein processing and consequently diminish the vitality of aging lotus seeds. Moreover, an expansion in the number of seed maturation and defense response genes was observed in the lotus genome compared with 11 other species, which might represent an adaptive strategy against long-term adverse storage conditions. Overall, these findings are crucial for understanding the mechanisms underlying lotus seed longevity and may inform future improvements in the extended storage periods of seed crops.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1147-1163"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Date palm diverts organic solutes for root osmotic adjustment and protects leaves from oxidative damage in early drought acclimation. 在早期干旱适应过程中，枣椰树将有机溶质用于根部渗透调节，并保护叶片免受氧化损伤。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae456

Bastian L Franzisky, Heike M Mueller, Baoguo Du, Thomas Lux, Philip J White, Sebastien Christian Carpentier, Jana Barbro Winkler, Joerg-Peter Schnitzler, Jörg Kudla, Jaakko Kangasjärvi, Michael Reichelt, Axel Mithöfer, Klaus F X Mayer, Heinz Rennenberg, Peter Ache, Rainer Hedrich, Maxim Messerer, Christoph-Martin Geilfus

{"title":"Date palm diverts organic solutes for root osmotic adjustment and protects leaves from oxidative damage in early drought acclimation.","authors":"Bastian L Franzisky, Heike M Mueller, Baoguo Du, Thomas Lux, Philip J White, Sebastien Christian Carpentier, Jana Barbro Winkler, Joerg-Peter Schnitzler, Jörg Kudla, Jaakko Kangasjärvi, Michael Reichelt, Axel Mithöfer, Klaus F X Mayer, Heinz Rennenberg, Peter Ache, Rainer Hedrich, Maxim Messerer, Christoph-Martin Geilfus","doi":"10.1093/jxb/erae456","DOIUrl":"10.1093/jxb/erae456","url":null,"abstract":"Date palm (Phoenix dactylifera L.) is an important crop in arid regions and it is well adapted to desert ecosystems. To understand its remarkable ability to grow and yield in water-limited environments, we conducted experiments in which water was withheld for up to 4 weeks. In response to drought, root, rather than leaf, osmotic strength increased, with organic solutes such as sugars and amino acids contributing more to the osmolyte increase than minerals. Consistently, carbon and amino acid metabolism was acclimated toward biosynthesis at both the transcriptional and translational levels. In leaves, a remodeling of membrane systems was observed, suggesting changes in thylakoid lipid composition which, together with the restructuring of the photosynthetic apparatus, indicated an acclimation preventing oxidative damage. Thus, xerophilic date palm avoids oxidative damage under drought by combined prevention and rapid detoxification of oxygen radicals. Although minerals were expected to serve as cheap key osmotics, date palm also relies on organic osmolytes for osmotic adjustment in the roots during early drought acclimation. The diversion of these resources away from growth is consistent with the date palm strategy of generally slow growth in harsh environments and clearly indicates a trade-off between growth and stress-related physiological responses.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1244-1265"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accounting for the impact of genotype and environment on variation in leaf respiration of wheat in Mexico and Australia. 墨西哥和澳大利亚小麦的基因型和环境对叶片呼吸变化的影响。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae449

Oorbessy Gaju, Keith J Bloomfield, Anna C A Negrini, Andrew F Bowerman, Darren Cullerne, Bradley Cooper Posch, Callum Bryant, Yuzhen Fan, Matthew Spence, Bethany Stone, Matthew Gilliham, Robert T Furbank, Gemma Molero, Barry J Pogson, Ky Mathews, A Harvey Millar, Allison L Pearson, Matthew P Reynolds, Elke Stroeher, Nicolas L Taylor, Matthew H Turnbull, Owen K Atkin

{"title":"Accounting for the impact of genotype and environment on variation in leaf respiration of wheat in Mexico and Australia.","authors":"Oorbessy Gaju, Keith J Bloomfield, Anna C A Negrini, Andrew F Bowerman, Darren Cullerne, Bradley Cooper Posch, Callum Bryant, Yuzhen Fan, Matthew Spence, Bethany Stone, Matthew Gilliham, Robert T Furbank, Gemma Molero, Barry J Pogson, Ky Mathews, A Harvey Millar, Allison L Pearson, Matthew P Reynolds, Elke Stroeher, Nicolas L Taylor, Matthew H Turnbull, Owen K Atkin","doi":"10.1093/jxb/erae449","DOIUrl":"10.1093/jxb/erae449","url":null,"abstract":"An approach to improving radiation use efficiency (RUE) in wheat is to screen for variability in rates of leaf respiration in darkness (Rdark). We used a high-throughput system to quantify variation in Rdark among a diverse range of spring wheat genotypes (301 lines) grown in two countries (Mexico and Australia) and two seasons (2017 and 2018), and in doing so quantify the relative importance of genotype (G) and environment (E) in influencing variations in leaf Rdark. Through careful design, residual (unexplained) variation represented <10% of the total observed. Up to a third of the variation in Rdark (and related traits) was under genetic control. This suggests opportunities for breeders to use Rdark as a novel selection tool. In addition, E accounted for more than half of the total variation in area-based rates of Rdark. Here, the day of measurement was crucial, suggesting that day-to-day variations in the environment influence rates of Rdark measured at a common temperature. Overall, this study provides new insights into the role G and E play in determining variation in rates of leaf Rdark of one of the most important cereal crops, with implications for future improvements in carbon use efficiency and yield.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1099-1115"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Betaine lipids overproduced in seed plants are not imported into plastid membranes and promote endomembrane expansion. 种子植物中过量产生的甜菜碱脂类被排除在质体膜之外，并促进内膜扩张。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae458

Sarah Salomon, Marion Schilling, Catherine Albrieux, Grégory Si Larbi, Pierre-Henri Jouneau, Sylvaine Roy, Denis Falconet, Morgane Michaud, Juliette Jouhet

{"title":"Betaine lipids overproduced in seed plants are not imported into plastid membranes and promote endomembrane expansion.","authors":"Sarah Salomon, Marion Schilling, Catherine Albrieux, Grégory Si Larbi, Pierre-Henri Jouneau, Sylvaine Roy, Denis Falconet, Morgane Michaud, Juliette Jouhet","doi":"10.1093/jxb/erae458","DOIUrl":"10.1093/jxb/erae458","url":null,"abstract":"Plants and algae have to adapt to environmental changes and face various stresses that negatively affect their growth and development. One common stress is phosphate (Pi) deficiency, which is often present in the environment at limiting levels. In response to Pi deficiency, these organisms increase Pi uptake and remobilize intracellular Pi. Phospholipids are degraded to provide Pi and are replaced by non-phosphorus lipids, such as glycolipids or betaine lipids. During evolution, seed plants lost the ability to synthesize betaine lipids. By expressing Bta1 genes, which are involved in the synthesis of diacylglyceryl-N,N,N-trimethyl-homoserine (DGTS), from different species, we showed that DGTS can be produced in seed plants. In Arabidopsis, expression of BTA1 under a phosphate starvation-inducible promoter resulted in limited DGTS production without having any impact on plant growth or lipid remodelling. In transient expression systems in Nicotiana benthamiana, leaves were able to accumulate DGTS to up to 30% of their glycerolipid content at a slight expense to galactolipid and phospholipid production. At the subcellular level, we showed that DGTS is absent from plastids and seems to be enriched in endomembranes, inducing endoplasmic reticulum membrane proliferation. Finally, the DGTS synthesis pathway seems to compete with phosphatidylcholine (PC) synthesis via the Kennedy pathway but does not appear to be derived from the PC diacylglycerol backbone and therefore does not interfere with the eukaryotic pathway involved in galactolipid synthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"980-996"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ethylene modulates wheat response to phosphate deficiency. 乙烯调节小麦对磷酸盐缺乏的反应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae483

Ruonan Wang, Andrew F Bowerman, Yinglong Chen, Lu Zheng, Renfang Shen, Barry Pogson, Ping Lan

{"title":"Ethylene modulates wheat response to phosphate deficiency.","authors":"Ruonan Wang, Andrew F Bowerman, Yinglong Chen, Lu Zheng, Renfang Shen, Barry Pogson, Ping Lan","doi":"10.1093/jxb/erae483","DOIUrl":"10.1093/jxb/erae483","url":null,"abstract":"Ethylene is involved in the response to P deficiency in some model plants such as Arabidopsis and rice, but its role in wheat remains unclear. Following our recent study demonstrating the role of differentially expressed genes encoding ethylene response factors (ERFs) in response to P starvation in wheat, this study aims to investigate remodeling of the ethylene pathway and the physiological roles of ethylene in wheat under P deficiency using transcriptome analysis and the addition of the exogenous ethylene analogue, ethephon, or ethylene inhibitors. ERFs with at least a 2-fold expression change upon P deficiency had a distribution biased towards chromosome 4B. A group of genes encoding aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase were up-regulated under P starvation, suggesting an increase in ACC and ethylene content, which was verified by biochemical measurements and gas chromatography-mass spectrometry analysis. Under P deficiency, both root and shoot biomass decreased with application of exogenous ethephon or ethylene inhibitors, while root fork numbers and root surface area decreased upon ethephon treatment. Phosphate (Pi) concentrations in roots and old leaves increased with ethephon treatment, and Pi redistribution in roots and younger leaves was altered under Pi starvation. Our findings can guide breeding of germplasm with high Pi efficiency.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1314-1332"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The BnamiR827-BnaA09.NLA1-BnaPHT1 module regulates phosphate homeostasis, pollen viability, and seed yield in Brassica napus. 监管模块BnamiR827-BnaA09。NLA1-BnaPHT1s调控甘蓝型油菜磷酸盐稳态、花粉活力和种子产量。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae484

Tao Wu, Bei Han, Yajie Wang, Bingbing Zhang, Chuang Wang, Sheliang Wang, Hongmei Cai, Zhu Liu, John P Hammond, Surya Kant, Guangda Ding, Fangsen Xu, Lei Shi

{"title":"The BnamiR827-BnaA09.NLA1-BnaPHT1 module regulates phosphate homeostasis, pollen viability, and seed yield in Brassica napus.","authors":"Tao Wu, Bei Han, Yajie Wang, Bingbing Zhang, Chuang Wang, Sheliang Wang, Hongmei Cai, Zhu Liu, John P Hammond, Surya Kant, Guangda Ding, Fangsen Xu, Lei Shi","doi":"10.1093/jxb/erae484","DOIUrl":"10.1093/jxb/erae484","url":null,"abstract":"Phosphorus (P) is an essential macronutrient for the growth and yield of crops. However, there is limited understanding of the regulatory mechanisms of phosphate (Pi) homeostasis, and its impact on growth, development, and yield-related traits in Brassica napus. Here, we identified four NITROGEN LIMITATION ADAPTATION1 (BnaNLA1) genes in B. napus; their expression was predominant in roots and suppressed by Pi starvation-induced BnamiR827. All the BnaNLA1 proteins have similar sequences, subcellular localizations, and abilities to rescue the growth defects of the atnla1 mutant. One of the genes, BnaA09.NLA1, is expressed abundantly in roots, and also in old leaves, anthers, and pollen. Knocking out BnaNLA1 genes or overexpressing BnamiR827 resulted in increased concentrations of Pi in leaves and stamens and reduced pollen viability, thereby negatively impacting seed yield. Bimolecular fluorescence complementation (BiFC) and split-ubiquitin yeast two-hybrid (Y2H) analyses demonstrated that BnaA09.NLA1 interacted with seven Pi transporters highly expressed in roots and/or anthers (i.e. BnaPT8/10/11/27/35/37/42) to regulate Pi uptake and Pi allocation in anthers. Taken together, this study demonstrates that the BnamiR827-BnaA09.NLA1-BnaPHT1 module is involved in the regulation of Pi uptake and Pi allocation in floral organs, which is vital for the growth, pollen viability, and seed yield of B. napus.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1333-1350"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0