Journal of Experimental Botany最新文献_第8页

Sweet control: intracellular sorting of glycoproteins in plants. 甜控制：植物中糖蛋白的细胞内分选。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf095

Wencai Qi, Bingqi Zhang, Jiakai Yue, Liang Zhang

引用次数: 0

A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation. 毛杨叶芽中二氢查尔酮特异性o -甲基转移酶与芽树脂形成有关。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf020

Eerik-Mikael Piirtola, Dawei Ma, Jürgen Ehlting, C Peter Constabel

{"title":"A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation.","authors":"Eerik-Mikael Piirtola, Dawei Ma, Jürgen Ehlting, C Peter Constabel","doi":"10.1093/jxb/eraf020","DOIUrl":"10.1093/jxb/eraf020","url":null,"abstract":"Production of secreted leaf bud resin is a mechanism for temperate trees to protect dormant leaf buds against frost damage, dehydration, and insect herbivory. Bud resins contain a wide variety of special metabolites including terpenoids, benzenoids, and phenolics. The leaf bud resins of Populus trichocarpa and P. balsamifera contain high concentrations of O-methylated dihydrochalcones, but the enzymes for methylating these compounds remain enigmatic. We used transcriptomics and differential gene expression analyses to identify a gene encoding a P. trichocarpa dihydrochalcone-specific O-methyltransferase, which we named PtDOMT1. Detailed enzymatic analyses demonstrated PtDOMT1 to be a highly selective and regiospecific O-methyltransferase which methylates 4 and 4' positions of dihydrochalcones using S-adenosyl-l-methionine as a methyl donor. PtDOMT1 did not methylate any other flavonoid or phenolic substrate tested. These findings establish the final step in the biosynthesis of O-methylated dihydrochalcones in poplar and represent the first molecular analysis of leaf bud resin biosynthesis in plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2129-2143"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LIPID RICH 1 modulates allocation of carbon between starch and triacylglycerol in Arabidopsis leaves. 脂质富1调节拟南芥叶片淀粉和甘油三酯之间的碳分配。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf048

Mebae Yamaguchi, Shuji Shigenobu, Katsushi Yamaguchi, Yasuhiro Higashi, Yozo Okazaki, Kazuki Saito, Emi Mishiro-Sato, Keiko Kano, Ryosuke Sugiyama, Mami Yamazaki, Shigeo S Sugano, Shuichi Fukuyoshi, Haruko Ueda, Ikuko Hara-Nishimura, Takashi L Shimada

{"title":"LIPID RICH 1 modulates allocation of carbon between starch and triacylglycerol in Arabidopsis leaves.","authors":"Mebae Yamaguchi, Shuji Shigenobu, Katsushi Yamaguchi, Yasuhiro Higashi, Yozo Okazaki, Kazuki Saito, Emi Mishiro-Sato, Keiko Kano, Ryosuke Sugiyama, Mami Yamazaki, Shigeo S Sugano, Shuichi Fukuyoshi, Haruko Ueda, Ikuko Hara-Nishimura, Takashi L Shimada","doi":"10.1093/jxb/eraf048","DOIUrl":"10.1093/jxb/eraf048","url":null,"abstract":"Plants accumulate starch and triacylglycerols (TAGs) as carbon sources. Leaves primarily store starch in chloroplasts, with some TAGs stored in lipid droplets, but how carbon resource allocation is regulated in leaves during cellular metabolism is largely unknown. Using a forward genetics approach, we isolated an Arabidopsis thaliana mutant with more lipid droplets in its leaves than the wild type, named lipid rich 1 (liri1). The overaccumulation of lipid droplets was caused by loss of function in the causal gene, encoding an uncharacterized protein. TAG levels were 5-fold higher and starch levels 2-fold lower in the leaves of liri1 than in those of the wild type. LIRI1 localized to the chloroplasts, and contents of chloroplast membrane lipids were 20% higher in liri1 leaves than in wild-type leaves. Co-immunoprecipitation assays revealed that LIRI1 interacts with ACETYL-COENZYME A CARBOXYLASE CARBOXYLTRANSFERASE ALPHA SUBUNIT (an enzyme for fatty acid biosynthesis) and STARCH SYNTHASE 4 (an enzyme for starch biosynthesis). In isotope tracer experiments using [1-13C]sodium acetate, more 13C was incorporated into TAGs in liri1 leaves than in wild-type leaves. Moreover, liri1 plants showed growth defects and irregular chloroplasts. These results indicate that LIRI1 affects the carbon trade-off to inhibit lipid production in leaves.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2144-2161"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway. 大麦叶片-叶柄基因Uniculme4对植株结构和茉莉酸途径的多效性影响

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf068

Elahe Tavakol, Vahid Shariati, Irene Maria Fontana, Marta Binaghi, Hagen Stellmach, Bettina Hause, Gerit Bethke, Hatice Bilgic, Jayanand Boddu, Ron Okagaki, Shane Heinen, Gary J Muehlbauer, Laura Rossini

{"title":"Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway.","authors":"Elahe Tavakol, Vahid Shariati, Irene Maria Fontana, Marta Binaghi, Hagen Stellmach, Bettina Hause, Gerit Bethke, Hatice Bilgic, Jayanand Boddu, Ron Okagaki, Shane Heinen, Gary J Muehlbauer, Laura Rossini","doi":"10.1093/jxb/eraf068","DOIUrl":"10.1093/jxb/eraf068","url":null,"abstract":"Plant architecture is a key determinant of crop yield, and understanding the genetic basis of its regulation is crucial for crop improvement. BLADE-ON-PETIOLE (BOP) genes are known to play a fundamental role in shaping plant architecture across diverse species. In this study, we demonstrate pleiotropic effects of the barley BOP gene Uniculme4 (Cul4) on various aspects of plant architecture, including plant height, culm diameter, and grain traits. Accordingly, Cul4 is broadly expressed in different tissues and developmental stages. Comparing transcriptome profiles of cul4 mutant and wild-type plants, we uncover a novel link between Cul4 and the jasmonic acid (JA) biosynthetic pathway. Our findings demonstrate that proper Cul4 function is required to repress JA biosynthesis, with cul4 mutants exhibiting increased levels of JA and its precursor 12-oxo-phytodienoic acid. Up-regulation of WRKY and bHLH transcription factors shows JA signalling is also impacted by Cul4. Additionally, our study sheds light on the role of Cul4 in flowering time regulation, potentially through its interaction with florigen-like genes. This research enhances our understanding of the mechanisms and pathways acting downstream of BOP genes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2177-2191"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning of sulfur flow and sulfur seed metabolism in oilseed rape under sulfate-limited conditions. 硫酸盐限制条件下油菜硫流调控及硫粒代谢

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf028

Philippe D'Hooghe, Stanislav Kopriva, Jean-Christophe Avice, Jacques Trouverie

{"title":"Tuning of sulfur flow and sulfur seed metabolism in oilseed rape under sulfate-limited conditions.","authors":"Philippe D'Hooghe, Stanislav Kopriva, Jean-Christophe Avice, Jacques Trouverie","doi":"10.1093/jxb/eraf028","DOIUrl":"10.1093/jxb/eraf028","url":null,"abstract":"The response of oilseed rape to sulfur (S) restriction usually consists of increasing the components of S utilization efficiency (absorption, assimilation, and remobilization) to provide S to seeds. However, source-sink relationships and S management in developing seeds under sulfate restriction are poorly understood. To address this, impacts of sulfate restrictions applied at 'visible bud' or 'start of pod filling' stages were studied in two genotypes, Aviso and Capitol. The two cultivars have similar seed yield, but Capitol has higher seed weight and lower number of seeds per plant under non-limited conditions. S flow at the whole-plant level (using [34S]sulfate labelling) and S metabolism changes [S-compounds, and ATP sulfurylase and adenosine 5'-phosphosulfate reductase (APR) activities] were studied during seed development. Seed yield, protein quality, and accumulation of S metabolites were affected by sulfate restriction less and later in Aviso than in Capitol. This was related to higher S uptake and stronger remobilization of S from vegetative organs to seeds during early seed development in response to sulfate restriction. A higher seed APR activity was observed for Capitol in response to sulfate limitation, suggesting that APR is not limiting for sulfate assimilation and that seed S metabolism is principally devoted to S-amino acids and protein synthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2278-2296"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct domain regions of NIN and NLP1 mediate symbiotic and nitrate signalling in Medicago truncatula. 不同结构域的NIN和NLP1介导了苜蓿的共生和硝酸盐信号传导。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf037

Jiang Wang, Mengdi Fu, Zhenpeng Luo, Jing Liu, Fang Xie

{"title":"Distinct domain regions of NIN and NLP1 mediate symbiotic and nitrate signalling in Medicago truncatula.","authors":"Jiang Wang, Mengdi Fu, Zhenpeng Luo, Jing Liu, Fang Xie","doi":"10.1093/jxb/eraf037","DOIUrl":"10.1093/jxb/eraf037","url":null,"abstract":"Nodule Inception (NIN) and NIN-like protein 1 (NLP1), both belonging to the RWP-RK type transcription factors, play critical roles in plant development. Specifically, NIN is pivotal in facilitating root nodule symbiosis in nitrogen-starved conditions, while NLP1 coordinates nodulation in response to nitrate level. In this study, we conducted domain swapping experiments between NIN and NLP1 in Medicago truncatula to elucidate the functional significance of their respective domains. The findings reveal that the C-terminal regions, including the RWP-RK and PB1 domains of NIN, can substitute for those of NLP1, whereas reciprocal substitution does not yield equivalent outcomes. Moreover, our results emphasize the critical role of PB1-mediated interactions in the activity of NLP1, a feature not essential for NIN. Additionally, the N-terminal segment, conserved in NLPs but containing deletions or mutations in NIN, is essential for the proper functioning of both NIN and NLP1. Collectively, our research indicates evolutionary divergence of NIN from ancestral NLPs, suggesting specific adaptations that have enabled NIN as a central regulator in root nodulation processes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2297-2308"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice. 更正：单一功能性抗稻瘟病基因 Pi54 激活了水稻复杂的防御机制

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf054

引用次数: 0

CORONATINE INSENSITIVE 1-mediated repression of immunity-related genes in Arabidopsis roots is lifted upon infection with Verticillium longisporum. 冠状碱不敏感1介导的拟南芥根免疫相关基因的抑制在感染长孢黄萎病后被克服。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf056

Louisa Ulrich, Johanna Schmitz, Corinna Thurow, Christiane Gatz

{"title":"CORONATINE INSENSITIVE 1-mediated repression of immunity-related genes in Arabidopsis roots is lifted upon infection with Verticillium longisporum.","authors":"Louisa Ulrich, Johanna Schmitz, Corinna Thurow, Christiane Gatz","doi":"10.1093/jxb/eraf056","DOIUrl":"10.1093/jxb/eraf056","url":null,"abstract":"Verticillium longisporum is a soil-borne fungal pathogen that causes vascular disease predominantly in Brassicaceae. We have previously reported that the receptor of the plant defense hormone jasmonoyl-isoleucine (JA-Ile), CORONATINE INSENSITIVE 1 (COI1), is required in roots for the fungus to proliferate efficiently in the shoot, suggesting the presence of a mobile root-borne signal that influences the outcome of the disease in shoots. This function of COI1 in promoting susceptibility is independent of JA-Ile. To explore the underlying mechanisms, in this study we compared the root transcriptome of the Arabidopsis coi1 mutant with those of the susceptible JA-Ile-deficient allene oxide synthase (aos) mutant and the susceptible wild-type (WT). The biggest difference between the transcriptomes was due to 316 immunity-related genes that were constitutively higher expressed in coi1 as compared to the susceptible genotypes. Interfering with the expression of a sub-group of these genes partially suppressed the coi1-mediated tolerance phenotype. We therefore hypothesize that secreted defense compounds encoded by genes constitutively expressed in coi1 are transported to the shoot with the transpiration stream where they accumulate and interfere with fungal growth. In addition, we found that 149 of the 316 COI1-repressed genes were induced in the WT and aos upon infection, reaching similar expression levels as in mock-treated coi1. These were not further induced in coi1 upon infection. Thus, the repressive effect of COI1 is either lifted or overridden upon infection with V. longisporum.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2356-2372"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New crops on the block: effective strategies to broaden our food, fibre, and fuel repertoire in the face of increasingly volatile agricultural systems. 待售新作物：面对日益动荡的农业系统，扩大粮食、纤维和燃料储备的有效战略。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf023

Aaron Phillips, Carolyn J Schultz, Rachel A Burton

{"title":"New crops on the block: effective strategies to broaden our food, fibre, and fuel repertoire in the face of increasingly volatile agricultural systems.","authors":"Aaron Phillips, Carolyn J Schultz, Rachel A Burton","doi":"10.1093/jxb/eraf023","DOIUrl":"10.1093/jxb/eraf023","url":null,"abstract":"Climate change poses significant challenges to our ability to keep a growing global population fed, clothed, and fuelled. This review sets the scene by summarizing the impacts of climate change on production of the major grain crop species rice, wheat, and maize, with a focus on yield reductions due to abiotic stresses and altered disease pressures. We discuss efforts to improve resilience, emphasizing traits such as water use efficiency, heat tolerance, and disease resistance. We move on to exploring production trends of established, re-emerging, and new crops, highlighting the challenges of developing and maintaining new arrivals in the global market. We analyse the potential of wild relatives for improving domesticated crops, or as candidates for de novo domestication. The importance of pangenomes for uncovering genetic variation for crop improvement is also discussed. We examine the impact of climate change on non-cereals, including fruit, nut, and fibre crops, and the potential of alternative multiuse crops to increase global sustainability and address climate change-related challenges. Agave is used as an exemplar to demonstrate the strategic pathway for developing a robust new crop option. There is a need for sustained investment in research and development across the entire value chain to facilitate the exploration of diverse species and genetic resources to enhance crop resilience and adaptability to future environmental conditions.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2043-2063"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototropin 2 mediates daily cold priming to promote light responses in Arabidopsis. 促光素2介导拟南芥每日冷启动以促进光响应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf040

Minoru Noguchi, Issei Keino, Hitomi Takahashi, Shota Yamauchi, Mami Fujisawa, Ken Haga, Tatsuya Sakai, Atsushi Takemiya, Yutaka Kodama

{"title":"Phototropin 2 mediates daily cold priming to promote light responses in Arabidopsis.","authors":"Minoru Noguchi, Issei Keino, Hitomi Takahashi, Shota Yamauchi, Mami Fujisawa, Ken Haga, Tatsuya Sakai, Atsushi Takemiya, Yutaka Kodama","doi":"10.1093/jxb/eraf040","DOIUrl":"10.1093/jxb/eraf040","url":null,"abstract":"Organisms adapt to predictable environmental changes via a biological mechanism called priming. Phototropin is a plant-specific blue light photoreceptor that mediates daily light-induced responses, such as chloroplast relocation, stomatal opening, and phototropism, to optimize photosynthesis. Phototropin also functions as a thermosensor for chloroplast relocation that may sense daily temperature decreases at night, thereby modulating light-induced responses at dawn; however, this hypothesis has not yet been fully explored. Here, we revealed that phototropin mediates daily cold priming to promote stomatal opening and phototropism in Arabidopsis under dawn-mimicking conditions. A cold pretreatment in the dark enhanced subsequent blue light-induced stomatal opening and phototropism at normal temperatures, suggesting that daily cold priming is involved in these physiological responses. Arabidopsis has two phototropin proteins (phot1 and phot2), and we showed that phot2 clearly mediates cold priming of stomatal opening and phototropism. Cold priming appears to be based on phototropin-mediated thermosensing just before dawn, which plants use to optimize their light-induced responses in anticipation of dawn.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2309-2319"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0