Journal of Experimental Botany最新文献_第8页

New crops on the block: effective strategies to broaden our food, fibre and fuel repertoire in the face of increasingly volatile agricultural systems.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-27 DOI: 10.1093/jxb/eraf023

Aaron Phillips, Carolyn J Schultz, Rachel A Burton

{"title":"New crops on the block: effective strategies to broaden our food, fibre and fuel repertoire in the face of increasingly volatile agricultural systems.","authors":"Aaron Phillips, Carolyn J Schultz, Rachel A Burton","doi":"10.1093/jxb/eraf023","DOIUrl":"https://doi.org/10.1093/jxb/eraf023","url":null,"abstract":"Climate change poses significant challenges to our ability to keep a growing global population fed, clothed, and fuelled. This review sets the scene by summarising the impacts of climate change on production of the major grain crop species rice, wheat, and maize, with a focus on yield reductions due to abiotic stresses and altered disease pressures. We discuss efforts to improve resilience, emphasising traits such as water use efficiency (WUE), heat tolerance, and disease resistance. We move on to exploring production trends of established, re-emerging, and new crops, highlighting the challenges of developing and maintaining new arrivals in the global market. We analyse the potential of wild relatives for improving domesticated crops, or as candidates for de novo domestication. The importance of pangenomes for uncovering genetic variation for crop improvement is also discussed. We examine the impact of climate change on non-cereals, including fruit, nut, and fibre crops and the potential of alternative multiuse crops to increase global sustainability and address climate change-related challenges. Agave is used as an exemplar to demonstrate the strategic pathway for developing a robust new crop option. There is a need for sustained investment in research and development across the entire value chain to facilitate the exploration of diverse species and genetic resources to enhance crop resilience and adaptability to future environmental conditions.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of MLO trafficking by calmodulin binding domains.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-26 DOI: 10.1093/jxb/eraf047

Jing Yuan, Sienna T Ogawa, Daniel S Jones, Noel Lucca, Yan Ju, Sharon A Kessler

{"title":"Regulation of MLO trafficking by calmodulin binding domains.","authors":"Jing Yuan, Sienna T Ogawa, Daniel S Jones, Noel Lucca, Yan Ju, Sharon A Kessler","doi":"10.1093/jxb/eraf047","DOIUrl":"https://doi.org/10.1093/jxb/eraf047","url":null,"abstract":"Flowering plant sexual reproduction relies on the communication between the pollen tube and synergid cells to induce pollen tube bursting. During this process, the MILDEW RESISTANCE LOCUS-O (MLO) protein NORTIA (NTA) is polarly trafficked from the Golgi, where it is inactive, to the filiform apparatus, where it is functional in synergids. MLOs were recently described as calcium channels and have been proposed to be negatively regulated through calmodulin (CaM) binding at a conserved C-terminal calmodulin binding domain (CaMBD). To determine whether CaM binding is necessary for MLO function during pollen tube reception, C-terminal truncations and CaMBD point mutations were made in NTA. Point mutations were also generated in a constitutively filiform apparatus localized chimeric NTA containing the MLO1 C-terminus. In this study, we demonstrate that mutating the MLO1 and NTA CaMBD reduces the ability for MLOs to function during pollen tube reception. This is in part due to altered subcellular localization of the CaMBD mutants in synergids. We show that the CaMBD is not necessary for Golgi localization of MLOs, but is necessary for efficient trafficking and total protein accumulation at the filiform apparatus. Our results suggest an additional role for CaM binding as a regulator of MLO trafficking in addition to its previously proposed role as a negative regulator of MLO Ca2+ channel activity.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the agro-physiological potential of wheat rhizoplane fungi using a niche-conserved consortium construction approach with low P conditions.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-26 DOI: 10.1093/jxb/eraf042

Brahim Benbrik, Tessa E Reid, Dounia Nkir, Hicham Chaouki, Yassine Aallam, Ian M Clark, Tim H Mauchline, Jim Harris, Mark Pawlett, Abdellatif Barakat, Zineb Rchiad, Adnane Bargaz

{"title":"Unlocking the agro-physiological potential of wheat rhizoplane fungi using a niche-conserved consortium construction approach with low P conditions.","authors":"Brahim Benbrik, Tessa E Reid, Dounia Nkir, Hicham Chaouki, Yassine Aallam, Ian M Clark, Tim H Mauchline, Jim Harris, Mark Pawlett, Abdellatif Barakat, Zineb Rchiad, Adnane Bargaz","doi":"10.1093/jxb/eraf042","DOIUrl":"https://doi.org/10.1093/jxb/eraf042","url":null,"abstract":"Plant Growth Promoting Fungi (PGPF) hold promises for enhancing crop yield. This study delves into fungal diversity of the wheat rhizoplane across seven Moroccan agricultural regions, employing a niche-conserved strategy to construct fungal consortia (FC) exhibiting higher phosphorus (P) acquisition and plant growth promotion. This study combined culture-independent and culture-dependent methods exploring taxonomic and functional diversity in the rhizoplane of wheat plants obtained from twenty-eight zones. Twenty fungal species from eight genera were isolated and confirmed through ITS Sanger sequencing. P solubilization (PS) capacity was assessed for individual species, with Talaromyces sp. (F11) and Rhizopus arrhizus CMRC 585 (F12) exhibiting notable PS rates, potentially due to organic acid production like gluconic acid. PGP traits and antagonism activities were considered when constructing twenty-eight niche-conserved FC (using isolates from the same zone), seven intra-region FC (different zones within a region), and one inter-regions FC. Under low P conditions, in-planta inoculation with niche-conserved FC (notably FC14 and FC17) enhanced growth, physiological parameters and P uptake of wheat, in both vegetative and reproductive stages. FC14 and FC17, composed of potent fungi such as F11 and F12, demonstrated superior plant growth benefits compared to intra- and inter-regions constructed FC. Our study underscores the efficacy of the niche-conserved strategy in constructing FC from isolates within the same niche, proving significant agro-physiological potential to enhance P uptake and plant growth of wheat.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-Aminobutyric acid-induced resistance in postharvest peach fruit involves interaction between the MAPK cascade and SNARE13 protein in the salicylic acid-dependent pathway. β-氨基丁酸诱导的采后桃果抗性涉及水杨酸依赖途径中的 MAPK 级联和 SNARE13 蛋白之间的相互作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae448

Chunhong Li, Kaituo Wang, Changyi Lei, Yanyu Zou, Sisi Yang, Fei Xiang, Meilin Li, Yonghua Zheng

{"title":"β-Aminobutyric acid-induced resistance in postharvest peach fruit involves interaction between the MAPK cascade and SNARE13 protein in the salicylic acid-dependent pathway.","authors":"Chunhong Li, Kaituo Wang, Changyi Lei, Yanyu Zou, Sisi Yang, Fei Xiang, Meilin Li, Yonghua Zheng","doi":"10.1093/jxb/erae448","DOIUrl":"10.1093/jxb/erae448","url":null,"abstract":"The inducer β-aminobutyric acid (BABA) participates in the immune response in various plants. However, the specific mitogen-activated protein kinase (MAPK) cascade involved in BABA-induced resistance (BABA-IR) has not yet been elucidated. Here, peach (Prunus persica) fruits treated with the BABA exhibited pattern-triggered immunity defense against Rhizopus stolonifer, accompanied by the generation of reactive oxygen species and activation of a MAPK cascade. Transcriptome sequencing suggested that a total of 15 MAPK kinase kinase (PpMAPKKK)/MAPK kinase (PpMAPKK)/PpMAPK genes were involved in BABA-IR in peach fruit. Further qRT-PCR analysis showed that the transcript profiles of PpMAPKKK3, PpMAPKK5, and PpMAPK1 were elevated. Subsequently, yeast two-hybrid, luciferase complementation imaging, pull-down, and in vitro phosphorylation assays were conducted to characterize the complete MAPK cascade (PpMAPKKK3-PpMAPKK5-PpMAPK1) involved in peach fruit. Moreover, the downstream events of MAPK1 include the involvement of SNARE13 and the corresponding NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1)-responsive defense. Single silencing of MAPKKK3, MAPKK5, or MAPK1 and double silencing of MAPKKK3 and MAPKK5 or MAPKK5 and MAPK1 resulted in enhanced susceptibility to the fungus R. stolonifer in mutants and attenuated salicylic acid (SA)-dependent defense gene expression. In contrast, the homologous or heterologous overexpression of PpSNARE13 in peach fruit or Arabidopsis led to an enhanced SA pool and elevated expression of pathogenesis related (PR) genes. Reciprocally, the ppsnare13cas9 mutants were generally compromised in the priming of SA-dependent resistance. Therefore, the MAPKKK3-MAPKK5-MAPK1 cascade contributed to pattern-triggered immunity signal transduction in BABA-elicited peach fruit, by combination with downstream events such as SNARE13, NPR1, and SA-dependent signaling.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1202-1229"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis METHYLENETETRAHYDROFOLATE REDUCTASE 2 functions independently of PENETRATION 2 during primary immunity against rice blast. 拟南芥甲基乙二酸还原酶 2 在稻瘟病初级免疫过程中的功能独立于渗透 2。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae435

Eram Sultan, Debasish Pati, Sanjeev Kumar, Binod Bihari Sahu

{"title":"Arabidopsis METHYLENETETRAHYDROFOLATE REDUCTASE 2 functions independently of PENETRATION 2 during primary immunity against rice blast.","authors":"Eram Sultan, Debasish Pati, Sanjeev Kumar, Binod Bihari Sahu","doi":"10.1093/jxb/erae435","DOIUrl":"10.1093/jxb/erae435","url":null,"abstract":"Non-host resistance (NHR) is the most durable and robust form of innate immunity, with a surge of interest in its role in crop improvement. Of the NHR genes identified against rice blast, a devastating disease caused by Magnaporthe oryzae, Arabidopsis PEN2 is indispensable for pre-penetration resistance to M. oryzae, while a consortium of genes orchestrates post-penetration resistance via lesser known mechanisms. We identified M. oryzae-susceptible mosA (mthfr2 pen2-3) from a randomly mutagenized Arabidopsis pen2-3 population using forward genetics. Analysis of T-DNA-inserted mthfr2 lines and pen2-3-complemented mosA lines revealed that MTHFR2-dependent resistance to M. oryzae is independent of PEN2. MTHFR2-defective plants exhibited higher accumulation of reactive oxygen species and expression of salicylic acid-dependent defense markers. MTHFR2-ligand docking revealed that A55V non-synonymous substitution in mosA altered ligand binding efficiency. This further affected the metabolomic profile of mosA, effectively allowing in vitro germination and development of M. oryzae conidia. Moreover, the loss-of-function mutation in mthfr2 (involved in the 1C metabolic pathway) potentiated mosA immunity against Pst DC3000. In conclusion, our findings showed that MTHFR2 is a positive modulator of NHR against M. oryzae. This work documents another layer of conserved yet divergent metabolomic defense in Arabidopsis regulated by folate-mediated 1C metabolism that has the potential to revolutionize crop improvement.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1032-1048"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochrome-interacting factors PIF4 and PIF5 directly regulate autophagy during leaf senescence in Arabidopsis. 拟南芥叶片衰老过程中植物色素相互作用因子PIF4和PIF5直接调控自噬。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae469

Juhyeon Lee, Myeong Hoon Kang, Da-Min Choi, Anne Marmagne, Jeehye Park, Heeho Lee, Eunha Gwak, Jong-Chan Lee, Jeong-Il Kim, Céline Masclaux-Daubresse, Pyung Ok Lim

{"title":"Phytochrome-interacting factors PIF4 and PIF5 directly regulate autophagy during leaf senescence in Arabidopsis.","authors":"Juhyeon Lee, Myeong Hoon Kang, Da-Min Choi, Anne Marmagne, Jeehye Park, Heeho Lee, Eunha Gwak, Jong-Chan Lee, Jeong-Il Kim, Céline Masclaux-Daubresse, Pyung Ok Lim","doi":"10.1093/jxb/erae469","DOIUrl":"10.1093/jxb/erae469","url":null,"abstract":"During leaf senescence, autophagy plays a critical role by removing damaged cellular components and participating in nutrient remobilization to sink organs. However, how AUTOPHAGY (ATG) genes are regulated during natural leaf senescence remains largely unknown. In this study, we attempted to identify upstream transcriptional regulator(s) of ATG genes and their molecular basis during leaf senescence in Arabidopsis through the combined analyses of promoter binding, autophagy flux, and genetic interactions. We found that PHYTOCHROME-INTERACTING FACTOR4 (PIF4) and PIF5 directly bind to the promoters of ATG5, ATG12a, ATG12b, ATG8a, ATG8e, ATG8f, and ATG8g, inducing their transcription. These target ATG genes are down-regulated in pif4, pif5, and pif4pif5 mutants, resulting in decreased autophagic activity and slower degradation of chloroplast proteins and chlorophyll. Conversely, overexpression of ATG8 genes accelerated protein degradation with early leaf senescence. Moreover, our data suggested partial suppression of the pif4pif5 phenotype by ATG8a overexpression. PIF4/PIF5 also influence senescence induced by nutrient starvation, another hallmark of the autophagy pathway. Furthermore, we observed that the PIF4/PIF5-ATG regulatory module may contribute to seed maturation. Our study not only unveils transcriptional regulators of autophagy in natural leaf senescence but also underscores the potential role of PIF4/PIF5 as functional regulators in leaf senescence and nutrient remobilization.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1068-1084"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tool to measure maize root system stiffness that enables a comprehensive understanding of plant mechanics and lodging.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae465

Ashley N Hostetler, Jonathan W Reneau, Joseph Cristiano, Teclemariam Weldekidan, Taran A Kermani, Therese T Kim, Erin E Sparks

{"title":"A tool to measure maize root system stiffness that enables a comprehensive understanding of plant mechanics and lodging.","authors":"Ashley N Hostetler, Jonathan W Reneau, Joseph Cristiano, Teclemariam Weldekidan, Taran A Kermani, Therese T Kim, Erin E Sparks","doi":"10.1093/jxb/erae465","DOIUrl":"10.1093/jxb/erae465","url":null,"abstract":"Plant mechanical failure, known as lodging, has detrimental impacts on the quality and quantity of maize yields. Failure can occur at stalks (stalk lodging) or at roots (root lodging). While previous research has focused on proxy measures for stalk stiffness, stalk strength, and root strength, there is a need to quantify the root system stiffness, which quantifies the force-displacement relationship. Here, we report a tool to quantify the root system stiffness of maize hybrids grown in different conditions. The results show that maize hybrids with a higher root system stiffness have a greater susceptibility to root lodging. This result is consistent with expected mechanical behavior, since higher root system stiffness values mean that the plant reaches the failure strength at lower displacements compared with a plant with lower root system stiffness. Collectively, this study describes the first tool to measure root system stiffness and enables a comprehensive understanding of the integrated plant mechanics and lodging.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"950-960"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A quest for the potato of the future: characterization of wild tuber-bearing Solanum species for de novo domestication. 探索未来的马铃薯：对野生块茎茄科植物进行特性鉴定，以便重新驯化。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae453

Aristotelis Azariadis, Sara Miller Johansen, Olga A Andrzejczak, Harsh Yadav, Zeinu M Belew, Wen Xia, Christoph Crocoll, Andreas Blennow, Henrik Brinch-Pedersen, Bent L Petersen, Hussam H Nour-Eldin, Kim H Hebelstrup

{"title":"A quest for the potato of the future: characterization of wild tuber-bearing Solanum species for de novo domestication.","authors":"Aristotelis Azariadis, Sara Miller Johansen, Olga A Andrzejczak, Harsh Yadav, Zeinu M Belew, Wen Xia, Christoph Crocoll, Andreas Blennow, Henrik Brinch-Pedersen, Bent L Petersen, Hussam H Nour-Eldin, Kim H Hebelstrup","doi":"10.1093/jxb/erae453","DOIUrl":"10.1093/jxb/erae453","url":null,"abstract":"Potato (Solanum tuberosum) is a staple food worldwide, but modern potato cultivation relies heavily on the use of pesticides to control pests and diseases. However, many wild Solanum species are highly resistant to biotic and abiotic stresses relevant to potato production. Several of those species have been used in potato breeding to confer resistance but this has only been moderately successful. Instead, we propose an alternative approach to utilize the potential of wild Solanum germplasm. Recently, de novo domestication has been suggested to produce more resilient crops: instead of introducing resistance genes into existing crops, domestication traits could be introduced into resistant wild crop relatives to create new crops. Therefore, we selected 10 promising species from the 107 known wild tuber-bearing Solanum species for their resistance to biotic and abiotic stresses. Selection was based on the existing literature, characterizing species by tuberization under short- and long-day conditions, tuber glycoalkaloid content, starch digestibility and performance in tissue culture. Based on this, the highly pest- and disease-resistant S. bulbocastanaum was chosen. Our results showed that it produced relatively large tubers, also under long-day conditions, and performed exceptionally well in tissue culture.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1011-1031"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factors CsWRKY53 and CsWRKY40 synergistically regulate l-theanine hydrolysis via the abscisic acid signaling pathway during tea withering. CsWRKY53和CsWRKY40通过脱落酸信号途径协同调控茶叶萎凋时L-茶氨酸的水解。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae460

Haiyan Cheng, Qianhong Pan, Wei Wu, Jimin Shen, Xiaofen Liu, Yuxuan Shi, Xueren Yin, Ping Xu

引用次数: 0

The PEBP genes FLOWERING LOCUS T and TERMINAL FLOWER 1 modulate seed dormancy and size. PEBP基因开花位点T和顶花1调控种子的休眠和大小。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-25 DOI: 10.1093/jxb/erae466

Judit Nadal Bigas, Martijn Fiers, Froukje van der Wal, Leo A J Willems, Viola Willemsen, Harm Nijveen, Gerco C Angenent, Richard G H Immink

{"title":"The PEBP genes FLOWERING LOCUS T and TERMINAL FLOWER 1 modulate seed dormancy and size.","authors":"Judit Nadal Bigas, Martijn Fiers, Froukje van der Wal, Leo A J Willems, Viola Willemsen, Harm Nijveen, Gerco C Angenent, Richard G H Immink","doi":"10.1093/jxb/erae466","DOIUrl":"10.1093/jxb/erae466","url":null,"abstract":"The phosphatidylethanolamine-binding protein (PEBP) family members FLOWERING LOCUS T (FT) and TERMINAL FLOWER1 (TFL1) are major regulators of plant reproduction. In Arabidopsis, the FT/TFL1 balance defines the timing of floral transition and the determination of inflorescence meristem identity. However, emerging studies have elucidated a plethora of previously unknown functions for these genes in various physiological processes. Here, we characterized potential roles in seed size and dormancy of FT and TFL1 in Arabidopsis thaliana using CRISPR mutants and reporter analysis. Our findings unveiled a role for TFL1 in seed dormancy while confirming the role of FT in regulating this trait. We showed that the interplay between these two genes in seed dormancy is antagonistic, mirroring their roles in flowering time and inflorescence architecture. Analysis of reporter lines demonstrated that FT and TFL1 are partly co-expressed in seeds. Finally, we showed that total seed yield is affected in these mutants. Together, our results highlight the versatility of these two genes beyond their canonical functions. The impact of FT and TFL1 on seed characteristics emphasizes the significance of approaching gene studies from various perspectives, enabling the identification of multifaceted molecular factors that could play a major role in shaping the future of agriculture.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1049-1067"},"PeriodicalIF":5.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0