Journal of Experimental Botany最新文献

From Biosynthesis to Signaling: Unveiling the Multifaceted Roles of Phytosulfokine Peptide in Plants. 从生物合成到信号传导：揭示植物磺胺肽在植物中的多重作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-23 DOI: 10.1093/jxb/eraf230

Changqi Wu, Raheel Munir, Fangfang Li, Pingfang Li, Yi Cai, Kai Shi

引用次数: 0

Salicylic acid-induced stomatal closure differs in vascular plants of various lineages. 水杨酸诱导的气孔关闭在不同系维管植物中是不同的。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf227

Xiayi Zhang, Guoliang Chen, Xudong Liu, Minhui Bi, Shihua Qi, Yuanyuan Zeng, Yanru Li, Yaxian Du, Xueqian Tian, Ruiyun He, Xiangwen Fang

{"title":"Salicylic acid-induced stomatal closure differs in vascular plants of various lineages.","authors":"Xiayi Zhang, Guoliang Chen, Xudong Liu, Minhui Bi, Shihua Qi, Yuanyuan Zeng, Yanru Li, Yaxian Du, Xueqian Tian, Ruiyun He, Xiangwen Fang","doi":"10.1093/jxb/eraf227","DOIUrl":"https://doi.org/10.1093/jxb/eraf227","url":null,"abstract":"Salicylic acid (SA)-triggering stomatal closure to prevent pathogen invasion has been widely recognized in angiosperms. However, whether SA-induced stomal closure differs across vascular plants of various lineages remains unclear. In this study, the stomatal responses of 28 species, including 13 angiosperms, 6 gymnosperms, 8 ferns, and 1 lycophyte, to exogenous SA were evaluated, and the stomatal closure signaling pathways of 6 representative species were subsequently examined. SA-induced stomatal closure was observed in seed plants but not in the ferns or lycophyte. Additionally, SA induced an increase in ROS in the guard cells of these 6 different vascular plants, but NO accumulation in and K+ efflux from guard cells were detected in seed plants but not in the 2 ferns or 1 lycophyte investigated. The application of SA induced large vacuolar compartments in the guard cells of the seed plants to disaggregate, whereas no such changes were observed in the guard cells of the ferns or lycophyte. The differences in the responses of the seed plant, fern, and lycophyte stomata to SA observed in this study were due mainly to the partial absence of the SA signaling pathway in the ferns and lycophyte.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interference with phytohormone signaling by whiteflies differentially affects a plant's attractiveness to a larval and an egg parasitoid of the cabbage white butterfly. 白蝇对植物激素信号的干扰不同程度地影响了植物对白菜白蝴蝶幼虫和卵类寄生虫的吸引力。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf208

Yu-Mei Dong, Ya-Li Sang, Shu-Zhen Wang, Ted C J Turlings, Ye-Hua Li, Da-Wei Xue, Peng-Jun Zhang

{"title":"Interference with phytohormone signaling by whiteflies differentially affects a plant's attractiveness to a larval and an egg parasitoid of the cabbage white butterfly.","authors":"Yu-Mei Dong, Ya-Li Sang, Shu-Zhen Wang, Ted C J Turlings, Ye-Hua Li, Da-Wei Xue, Peng-Jun Zhang","doi":"10.1093/jxb/eraf208","DOIUrl":"https://doi.org/10.1093/jxb/eraf208","url":null,"abstract":"In response to egg deposition or feeding by insect herbivores plants release specific blends of volatiles that attract natural enemies of the herbivores. In nature, plants are often simultaneously attacked by multiple herbivores that may induce different signaling pathways, thus affecting the volatile blends and interfering with the attraction of the natural enemies. The mechanisms underlying such interference remain largely unknown. Here, we show that co-infestation of Arabidopsis thaliana plants with the whitefly Bemisia tabaci reduces the volatile emissions induced by Pieris rapae caterpillars, resulting in reduced attraction of the larval parasitoid Cotesia rubecula. Hormone and gene expression analyses, followed by assays with various Arabidopsis mutants revealed that this interference by B. tabaci is the result of antagonistic crosstalk between jasmonic acid (JA) and salicylic acid (SA), involving the transcription factor NPR1 but not WRKY70. In contrast, B. tabaci co-infestation had no impact on the attraction of an egg parasitoid to P. rapae egg-induced volatiles. These differential effects of the whitefly on the behavior of the two parasitoids were confirmed in greenhouse assays. This study provides new insight into the molecular mechanisms underlying the recruitment of different parasitoids by plants and could help in developing effective biocontrol strategies.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Small RNA mobility and plant virus diseases. 小RNA迁移与植物病毒病。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf226

Laura Elvira-Gonzalez, Todd Blevins, Manfred Heinlein

引用次数: 0

What metabolomics has taught us about tomato fruit ripening and quality. 代谢组学告诉我们番茄果实的成熟和质量。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf209

Esra Karakas, Alisdair R Fernie

引用次数: 0

Age-dependent differential iron deficiency responses of rosette leaves during reproductive stages in Arabidopsis thaliana. 拟南芥莲座叶片在繁殖阶段的年龄依赖性差异缺铁反应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-21 DOI: 10.1093/jxb/eraf207

Mary Ngigi, Mather Khan, Ricarda Remus, Shishir K Gupta, Petra Bauer

{"title":"Age-dependent differential iron deficiency responses of rosette leaves during reproductive stages in Arabidopsis thaliana.","authors":"Mary Ngigi, Mather Khan, Ricarda Remus, Shishir K Gupta, Petra Bauer","doi":"10.1093/jxb/eraf207","DOIUrl":"https://doi.org/10.1093/jxb/eraf207","url":null,"abstract":"Iron (Fe) is essential for plant development throughout the life cycle. Rosette leaves are responsive to Fe supply in Arabidopsis thaliana. Little is known about the dynamics of Fe deficiency (-Fe) responses of rosette leaves during the reproductive stages. We studied the dynamics of Fe-dependent responses at four consecutive reproductive stages (rosette, bolting, flowering, mature silique stages, hereby named RS, BS, FS, MS). We examined the growth of rosette leaves, elemental contents and gene expression patterns of Fe homeostasis genes belonging to differently regulated groups. We determined individual leaf sizes during seven days of +Fe and -Fe treatment at the RS. Young leaves responded to -Fe with growth inhibition and yellowing. Old and young leaves differed in gene expression patterns and elemental contents. Differences were noted between the early and late reproductive stages (primarily RS and BS versus MS) and correlations between ionomic contents and gene expression were detected. All leaves had induced Fe recycling genes at -Fe. Our findings highlight a developmental stage-dependent modulation of +Fe and -Fe responses in leaves. We discuss possible leaf signaling mechanisms accounting for the distinct responses between old and young leaves. This insight is informative to strengthen our understanding on plant iron management.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

13C labeling to determine intra-leaf photosynthetic heterogeneity dynamics during drought and rewatering. 13C标记测定干旱和复水期间叶片内光合异质性动态。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-20 DOI: 10.1093/jxb/eraf215

Junzhou Liu, Jinfang Zhao, Xiaoxia Ling, Dongliang Xiong

{"title":"13C labeling to determine intra-leaf photosynthetic heterogeneity dynamics during drought and rewatering.","authors":"Junzhou Liu, Jinfang Zhao, Xiaoxia Ling, Dongliang Xiong","doi":"10.1093/jxb/eraf215","DOIUrl":"https://doi.org/10.1093/jxb/eraf215","url":null,"abstract":"The spatial-temporal dynamics of photosynthetic heterogeneity within leaves under environmental fluctuations are still not well understood, limiting accurate assessments of plant photosynthetic capacity. Here, we combined 13CO2 labeling with water status monitoring to quantify variations in intra-leaf photosynthetic rate (A13C) during a drought-rewatering cycle. Hydraulic properties and anatomical traits were further investigated in well-watered plants. Under well-watered conditions, both A13C and water use efficiency (WUE) increased progressively from the leaf base to the tip. However, severe drought followed by rewatering eliminated this longitudinal gradient in A13C, with leaf tips exhibiting significantly impaired photosynthetic recovery. This impairment was associated with tip-specific limitations: 1) reduced water storage capacity exacerbating dehydration, and 2) increased vulnerability of hydraulic conductance potentially leading to hydraulic failure. Importantly, the impaired leaf tips can reduce whole-leaf WUE. Our findings demonstrate the utility of 13CO2 labeling for measuring leaf photosynthetic heterogeneity, reveal the dynamics of photosynthetic heterogeneity in response to environmental fluctuations, and highlight the vulnerability of leaf tips.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How to lose a whorl: the evolutionary and developmental biology of apetaly. 如何失去一个轮子：吸引力的进化和发育生物学。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-20 DOI: 10.1093/jxb/eraf211

Quentin C B Cronk

引用次数: 0

Deciphering the 3D structures of plant genomes: Building blocks of hierarchically organized chromatin domains. 破译植物基因组的三维结构：分层组织的染色质结构域的构建块。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-20 DOI: 10.1093/jxb/eraf218

Hongwoo Lee, Pil Joon Seo

{"title":"Deciphering the 3D structures of plant genomes: Building blocks of hierarchically organized chromatin domains.","authors":"Hongwoo Lee, Pil Joon Seo","doi":"10.1093/jxb/eraf218","DOIUrl":"https://doi.org/10.1093/jxb/eraf218","url":null,"abstract":"The spatial arrangement of chromatin within the nucleus is intricately regulated and acts as a key determinant of gene expression. Advanced high-resolution chromatin conformation capture techniques have revealed that plant genomes exhibit hierarchical organization within the nucleus, into large A and B compartments, intermediate topologically associating domain (TAD)-like domains, and fine gene-scale chromatin domains. In this review, we highlight recent findings demonstrating that TAD-like domains are closely associated with distinct epigenetic states, which are modulated by cohesin components. In addition, we underscore the significance of gene-scale chromatin domains, which are established by RNA polymerase II and accessible chromatin structures at gene borders. These fine-scale chromatin domains likely serve as the fundamental structural units for higher-order chromatin organization. Examining the chromatin structures at different levels of the hierarchy allows us to elucidate their epigenetic features and the molecular mechanisms for domain formation, providing insights into the three-dimensional organization of plant genomes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PHLOEM EARLY DOFs (PEARs) are induced within clubroot galls as a consequence of cambial stimulation and vascular reprogramming during Plasmodiophora brassicae infection in Arabidopsis thaliana. 韧皮部：在拟南芥侵染过程中，由于形成层刺激和血管重编程，在球茎瘿内诱导了早期DOFs（梨）。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-20 DOI: 10.1093/jxb/eraf225

Deeksha Singh, Sara Blicharz, Pawel Roszak, Ykä Helariutta, Robert Malinowski

{"title":"PHLOEM EARLY DOFs (PEARs) are induced within clubroot galls as a consequence of cambial stimulation and vascular reprogramming during Plasmodiophora brassicae infection in Arabidopsis thaliana.","authors":"Deeksha Singh, Sara Blicharz, Pawel Roszak, Ykä Helariutta, Robert Malinowski","doi":"10.1093/jxb/eraf225","DOIUrl":"https://doi.org/10.1093/jxb/eraf225","url":null,"abstract":"Clubroot galls represent complex cellular structures produced by Plasmodiophora brassicae, a soil-borne protist, which infects belowground parts of Brassicaceae plants, including Arabidopsis thaliana. This obligate biotroph hijacks host machinery to manipulate vascular development and patterning. The galls display a dramatic cellular reprogramming where phloem proliferation gets enhanced while xylogenesis is suppressed. Here, we show that at least three PHLOEM EARLY DOFs (PEARs) - PEAR1, DOF6 and HCA2 are induced within proliferating phloem during gall development. Mutation of these genes, however, does not decrease pathogen-driven phloem proliferation. To find upstream cues governing PEAR activities in galls, we compared auxin-cytokinin signalling responses within hypocotyls of mock- and P. brassicae-inoculated plants using fluorescent reporter lines and found strong auxin signalling responses within galls. We then modified auxin homeostasis in galls through chemical perturbation of its biosynthesis, transport and signalling, by employing synthetic inhibitors. These compounds drastically changed vascular development and patterning; however, they did not inhibit the induction of DOF6 promoter activity upon P. brassicae infection. Our results indicate that since PEARs promote cell proliferation in the phloem lineage, their induction reflects the altered proliferative status within the gall, rather than a direct impact of P. brassicae infection to overdrive phloem production.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144110702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0