Journal of Experimental Botany最新文献_第2页

Disruption of the Karrikin Receptor DWARF 14 LIKE (D14L) Gene Leads to Distinct Effects on Root and Shoot Growth, and Reprogramming of Central Metabolism in Rice. Karrikin受体DWARF 14 LIKE （D14L）基因的破坏对水稻根冠生长和中心代谢重编程的影响

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-13 DOI: 10.1093/jxb/eraf201

Mohamed A Salem, Muhammad Jamil, Jian You Wang, Lamis Berqdar, Kit Xi Liew, Anggra Paramita, Abdugaffor Ablazov, Aparna Balakrishna, Salim Al-Babili

{"title":"Disruption of the Karrikin Receptor DWARF 14 LIKE (D14L) Gene Leads to Distinct Effects on Root and Shoot Growth, and Reprogramming of Central Metabolism in Rice.","authors":"Mohamed A Salem, Muhammad Jamil, Jian You Wang, Lamis Berqdar, Kit Xi Liew, Anggra Paramita, Abdugaffor Ablazov, Aparna Balakrishna, Salim Al-Babili","doi":"10.1093/jxb/eraf201","DOIUrl":"https://doi.org/10.1093/jxb/eraf201","url":null,"abstract":"The rice (Oryza sativa L.) α/β hydrolase D14 LIKE (D14L), a paralog of the strigolactone receptor D14, is essential for the establishment of arbuscular mycorrhizal (AM) symbiosis and responses to karrikins, smoke-derived compounds that regulate several developmental processes. It is supposed that D14L is the receptor for a yet unidentified endogenous growth regulator. Herein, we determined the effects of disrupting the D14L on rice growth and metabolism. Our results revealed that the D14L loss of function altered rice architecture, leading to a significant increase in root growth and mesocotyl elongation, while hindering shoot growth, and a notable decrease in the number of tillers, especially under phosphate limiting conditions. Furthermore, d14l mutants exhibited significant reduction in the total grain yield. Metabolomics analysis revealed a notable shift of key metabolites, such as carbohydrates and amino acids involved in energy production and growth, from shoots to roots. This redistribution likely reflects an adaptive strategy to enhance nutrient acquisition and increase root biomass, albeit at the cost of shoot growth and productivity. Our findings highlight the pivotal role of D14L in regulating the root-to-shoot growth ratio and in coordinating the metabolism and allocation of resources across the rice plant.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abscisic acid and GIGANTEA signalling converge to regulate the recruitment of CONSTANS to the FT promoter and activate floral transition. 脱落酸和GIGANTEA信号汇聚调节CONSTANS向FT启动子的募集并激活花的转变。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-12 DOI: 10.1093/jxb/eraf199

Alice Robustelli Test, Giorgio Perrella, Beatrice Landoni, Sara Colanero, Aldo Sutti, Paolo Korwin Krukowski, Tianyuan Xu, Elisa Vellutini, Giulia Castorina, Massimo Galbiati, Damiano Martignago, Eirini Kaiserli, Chiara Tonelli, Lucio Conti

{"title":"Abscisic acid and GIGANTEA signalling converge to regulate the recruitment of CONSTANS to the FT promoter and activate floral transition.","authors":"Alice Robustelli Test, Giorgio Perrella, Beatrice Landoni, Sara Colanero, Aldo Sutti, Paolo Korwin Krukowski, Tianyuan Xu, Elisa Vellutini, Giulia Castorina, Massimo Galbiati, Damiano Martignago, Eirini Kaiserli, Chiara Tonelli, Lucio Conti","doi":"10.1093/jxb/eraf199","DOIUrl":"https://doi.org/10.1093/jxb/eraf199","url":null,"abstract":"Plants align flowering with optimal seasonal conditions to increase reproductive success. This process depends on modulating signalling pathways that respond to diverse environmental and hormonal inputs, thereby regulating the transition to flowering at the shoot apical meristem. In Arabidopsis, long-day photoperiods (LDs) stimulate the transcription of FLOWERING LOCUS T (FT), encoding the main florigenic signal. FT activation is mediated by the transcriptional regulator CONSTANS (CO), which binds to the CO responsive elements (COREs) located in the proximal FT promoter region. The phytohormone abscisic acid (ABA) also contributes to FT activation together with GIGANTEA (GI) to regulate drought escape (DE). Whether CO is a target of ABA and GI actions for the regulation of FT is, however, unknown. Here, we report that ABA and its signalling components promote CO recruitment to the CORE1/2, without causing reductions in the diel pattern of CO protein accumulation. ChIPseq analyses show that ABA broadly shapes the CO DNA binding landscape, which is enriched at the promoters of genes involved in the response to abiotic stress. We also found that GI promotes CO recruitment to the CORE1/2 region, and that CO recruitment is required for the accumulation of RNAPol II at the transcription start site of FT. Finally, we show that GI and ABA signalling pathways are largely epistatic in the control of flowering time, suggesting their involvement in the same molecular process. Taken together, these observations suggest that varying water deficit conditions modulate CO recruitment and FT expression, thus dictating DE strategies in Arabidopsis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144023404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide Signaling in Flowering Plants: Insights into Reproductive Thermotolerance. 开花植物的肽信号传导：生殖耐热性的见解。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-12 DOI: 10.1093/jxb/eraf192

Calvin J Perkins, Kelsey Pryze, Ravishankar Palanivelu

{"title":"Peptide Signaling in Flowering Plants: Insights into Reproductive Thermotolerance.","authors":"Calvin J Perkins, Kelsey Pryze, Ravishankar Palanivelu","doi":"10.1093/jxb/eraf192","DOIUrl":"https://doi.org/10.1093/jxb/eraf192","url":null,"abstract":"Flowering plants use small, secreted peptides to initiate complex signaling cascades that coordinate essential developmental processes and facilitate adaptation to environmental stresses. During plant reproduction, precise cell-cell interactions form the foundation of a tightly coordinated transition from sporophytic to gametophytic generation, culminating in pollen germination, tip growth, gamete fusion, and seed and fruit formation. While these reproductive processes have been studied from developmental and functional perspectives, emerging questions center on their vulnerability to rising global temperatures and heat-induced stress. By integrating insights from both research areas, this review comprehensively explores the diverse roles of small signaling peptides in plant reproduction and abiotic stress responses. While peptides are known regulators of reproductive developmental signaling, relatively few have been characterized for their roles in abiotic stress responses of reproductive tissues. To address this knowledge gap, we performed a meta-analysis of publicly available RNA-Seq data in reproductive tissues to identify candidate peptides in reproductive function and stress resilience. Our analysis highlights the altered expression of CLE and RALF gene families, identifying potential candidates for reproductive thermotolerance. Given their synthetic accessibility and ability to act when applied exogenously, small peptides present a promising avenue to enhance reproductive success and abiotic stress tolerance. These insights provide a framework for future functional studies and the development of targeted strategies to improve crop reproductive thermotolerance, offering a pathway to sustaining crop productivity under climate change.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRESSED FLOWER works downstream of ASYMMETRIC LEAVES 2 to affect sepal flatness in Arabidopsis. 压花作用于不对称叶片2下游，影响拟南芥萼片平整度。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-12 DOI: 10.1093/jxb/eraf200

Ruoyu Liu, Zeming Wang, Xi He, Heng Zhou, Yiru Xu, Lilan Hong

{"title":"PRESSED FLOWER works downstream of ASYMMETRIC LEAVES 2 to affect sepal flatness in Arabidopsis.","authors":"Ruoyu Liu, Zeming Wang, Xi He, Heng Zhou, Yiru Xu, Lilan Hong","doi":"10.1093/jxb/eraf200","DOIUrl":"https://doi.org/10.1093/jxb/eraf200","url":null,"abstract":"The development of flattened organs such as leaves and sepals is essential for proper plant function. While much research has focused on leaf flatness, little is known about how sepals achieve laminar organ morphology. Previous study has shown that in Arabidopsis an ASYMMETRIC LEAVES 2 (AS2) gene mutation as2-7D causes ectopic AS2 expression on the abaxial sepal epidermis, which leads to growth discoordination between the two sides of sepals, resulting in outgrowth formation on abaxial sepal epidermis and sepal flatness disruption. Here we report that the PRESSED FLOWER (PRS) works downstream of AS2 in affecting sepal flatness. Genetic analysis showed that PRS mutations suppressed the outgrowth formation on the abaxial sepal epidermis in as2-7D mutant. Through tracking the PRS expression dynamics at a cellular resolution throughout the early developmental stages in WT and as2-7D sepals, we found that on the abaxial epidermis of as2-7D sepals, ectopic AS2 expression up-regulated PRS expression, leading to the epidermal outgrowth initiation. AS2 affected PRS activity on multiple levels: AS2 activated PRS expression through direct binding to PRS promoter region; AS2 also physically interacted with PRS. Our study highlights the complex interplay between AS2 and PRS in modulating sepal flatness.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving spatially distinct phytohormone response zones in Arabidopsis thaliana roots colonized by Fusarium oxysporum. 解决尖孢镰刀菌定殖的拟南芥根系中不同空间的植物激素响应区。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-10 DOI: 10.1093/jxb/erae516

Jacob Calabria, Liu Wang, Madlen I Rast-Somssich, Hsiang-Wen Chen, Michelle Watt, Staffan Persson, Tonni Grube Andersen, Alexander Idnurm, Marc Somssich

{"title":"Resolving spatially distinct phytohormone response zones in Arabidopsis thaliana roots colonized by Fusarium oxysporum.","authors":"Jacob Calabria, Liu Wang, Madlen I Rast-Somssich, Hsiang-Wen Chen, Michelle Watt, Staffan Persson, Tonni Grube Andersen, Alexander Idnurm, Marc Somssich","doi":"10.1093/jxb/erae516","DOIUrl":"10.1093/jxb/erae516","url":null,"abstract":"Jasmonic acid (JA), ethylene (ET), and salicylic acid (SA) are the three major phytohormones coordinating plant defense responses, and all three are implicated in the defense against the fungal pathogen Fusarium oxysporum. However, their distinct modes of action and possible interactions remain unknown, in part because all spatial information on their activity is lacking. Here, we set out to probe this spatial aspect of plant immunity by using live microscopy with newly developed fluorescence-based transcriptional reporter lines. We have created a GreenGate vector collection of Plant Immune system Promoters (GG-PIPs) that allow us to image local activation of immune pathways with single-cell resolution. Using this system, we demonstrated that SA and JA act spatially separately from each other in distinct sets of root cells neighboring the fungal colonization site, while ET contributed to both sets. SA and ET induced the hypersensitive response as a first line of defense, while JA and ET governed active defense against the pathogen in a separate, second line of defense. Such an approach to resolve plant immune responses on an individual cell level has been lacking, and this work demonstrates that this microscopy-based approach can contribute to understanding plant immune responses in detail.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2022-2034"},"PeriodicalIF":5.6,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12066115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gibberellins: extending the Green Revolution. 赤霉素：扩展绿色革命。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-10 DOI: 10.1093/jxb/erae476

Janlo M Robil, Prameela Awale, Paula McSteen, Norman B Best

引用次数: 0

Salicylic acid accumulation: emerging molecular players and novel perspectives on plant development and nutrition. 水杨酸积累的最新见解：新出现的分子角色和植物发育与营养的新视角。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-10 DOI: 10.1093/jxb/erae309

Paula Salinas, Sebastián Velozo, Ariel Herrera-Vásquez

引用次数: 0

Plant hormones at the intersection of stress and development. 植物激素在胁迫和发育的交叉点。