Journal of Experimental Botany最新文献_第2页

The mitochondrial thioredoxin system regulates the TCA cycle-derived metabolic fluxes toward the GS/GOGAT cycle in illuminated leaves.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-24 DOI: 10.1093/jxb/eraf125

Paulo V L Souza, Vicente T C B Alencar, Humaira Bahadar, Joaquim Albenisio G Silveira, Danilo M Daloso

{"title":"The mitochondrial thioredoxin system regulates the TCA cycle-derived metabolic fluxes toward the GS/GOGAT cycle in illuminated leaves.","authors":"Paulo V L Souza, Vicente T C B Alencar, Humaira Bahadar, Joaquim Albenisio G Silveira, Danilo M Daloso","doi":"10.1093/jxb/eraf125","DOIUrl":"https://doi.org/10.1093/jxb/eraf125","url":null,"abstract":"Previous studies suggest that the synthesis of glutamate/glutamine is regulated by the mitochondrial thioredoxin (TRX) system. However, the mechanisms behind it remains unclear. Here, we demonstrated that the level of citrate and glutamate was higher in illuminated leaves from Arabidopsis mutants lacking the mitochondrial TRX o1 (trxo1) or both NADPH-dependent TRX reductases A/B (ntrab), that are found in nucleus, cytosol and mitochondria, when compared to the wild type (WT). Increased 13C-labelling in glutamate derived from 13C-pyruvate was observed in illuminated trxo1 and ntrab leaves, but not in WT and in the microsomal trxh2 mutant. The lack of TRX o1 decreased the content and activity of glutamine synthetase (GS), which leads to a lower level of glutamine, and exacerbated the increases in GS activity triggered by high light, when compared to the WT. The level of glutamine was positively correlated with the percentage of the oxidized GS band. However, the GS redox status was unaltered in all mutants. Our results indicate mitochondrial TRX mutants have higher metabolic fluxes from the TCA to the GS/GOGAT cycle in vivo, likely associated to an increased substrate availability and by direct-and-indirect TRX-mediated mechanisms that regulate enzymes of both TCA and GS/GOGAT cycles.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissection of photosynthetic short and long-term acclimation to fluctuating light reveals specific functions within the chloroplast thioredoxin network.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-24 DOI: 10.1093/jxb/eraf121

Dejan Dziubek, Louis Poeker, Luca Jäger, Martin Lehmann, Dario Leister, Ute Armbruster, Peter Geigenberger

{"title":"Dissection of photosynthetic short and long-term acclimation to fluctuating light reveals specific functions within the chloroplast thioredoxin network.","authors":"Dejan Dziubek, Louis Poeker, Luca Jäger, Martin Lehmann, Dario Leister, Ute Armbruster, Peter Geigenberger","doi":"10.1093/jxb/eraf121","DOIUrl":"https://doi.org/10.1093/jxb/eraf121","url":null,"abstract":"Thioredoxins (TRX) play an important role in controlling photosynthetic acclimation to fluctuating light (FL), while the underlying mechanisms remain unclear. Here we used Arabidopsis mutants lacking NADPH-dependent TRX-reductase C (NTRC) or parts of the light-dependent TRX systems to investigate specific functions of this network in FL. In the wildtype, photosynthetic acclimation required three days to develop and stabilized after five days of growth in FL. In the ntrc mutant, these acclimation responses were strongly attenuated, leading to decreased PSII acceptor availability, increased non-photochemical quenching (NPQ) and impaired PSII quantum efficiency. Moreover, PSI performance decreased, while acceptor-side limitation of PSI increased. This was due to a specifically strong decline in the reduction states of 2-Cys-peroxiredoxins and fructose-1,6-bisphosphatase, resulting in decreased CO2-fixation rates and delayed relaxation of NPQ in the low-light phases of FL. Since this was not reflected by changes in de-epoxidation of violaxanthin, state transition or chlorophyll a level, ntrc mutants showed an apparent lack of photoprotective responses that may explain their increased vulnerability under prolonged growth in FL. Results show that NADPH-dependent NTRC is balancing chloroplast redox-systems and Calvin-Benson-cycle activity during prolonged light variability, to optimize PSI, PSII and water-use efficiency, while having no direct effect on photoprotective mechanisms.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-21 DOI: 10.1093/jxb/eraf132

引用次数: 0

Using targeted genome methylation for crop improvement.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-20 DOI: 10.1093/jxb/eraf131

Zhibo Wang, Rebecca S Bart

{"title":"Using targeted genome methylation for crop improvement.","authors":"Zhibo Wang, Rebecca S Bart","doi":"10.1093/jxb/eraf131","DOIUrl":"https://doi.org/10.1093/jxb/eraf131","url":null,"abstract":"Genome editing allows scientists to specifically change the DNA sequence of an organism. This powerful technology now fuels basic biology discovery and tangible crop improvement efforts. There is a less well understood layer of information encoded in genomes, known collectively as 'epigenetics,' that impacts gene expression, without changing the DNA sequence. Epigenetic processes allow organisms to rapidly respond to environmental fluctuation. Like genome editing, recent advances have demonstrated that it is possible to edit the epigenome of a plant and cause heritable phenotypic changes. In this review we aim to specifically consider the unique advantages that targeted epigenome editing might provide over existing biotechnology tools. This review is aimed at a broad audience. We begin with a high-level overview the tools currently available for crop improvement. Next, we present a more detailed overview of the key discoveries that have been made in recent years, primarily using the model system Arabidopsis, new efforts to extend targeted methylation to crop plants, the current status of the technology and the challenges that remain to realize the full potential of targeted epigenome editing. We end with a forward-looking commentary on how epi-alleles might interface with breeding programs across a variety of crops.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing seed yield and nitrogen use efficiency of Brassica napus L. under low nitrogen by overexpression of G-proteins from Arabidopsis thaliana.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-19 DOI: 10.1093/jxb/eraf130

Yuyu Xie, Yunyou Nan, Ayub Atif, Derong Shi, Hui Tian, Jing Hui, Yanfeng Zhang, Alan M Jones, Yajun Gao

{"title":"Enhancing seed yield and nitrogen use efficiency of Brassica napus L. under low nitrogen by overexpression of G-proteins from Arabidopsis thaliana.","authors":"Yuyu Xie, Yunyou Nan, Ayub Atif, Derong Shi, Hui Tian, Jing Hui, Yanfeng Zhang, Alan M Jones, Yajun Gao","doi":"10.1093/jxb/eraf130","DOIUrl":"https://doi.org/10.1093/jxb/eraf130","url":null,"abstract":"Heterotrimeric G-proteins, composed of Gα, Gβ, and Gγ subunits, are involved in the regulation of multiple signaling pathways in plants. OsDEP1 (Gγ subunit-encoded protein) of rice and TaNBP1 (Gβ subunit-encoded protein) of wheat are homologs of Arabidopsis AGG3 and AGB1, respectively, which are regulators of grain size and also involved in nitrogen responses. However, the function of Arabidopsis G-proteins in nitrogen utilization under different nitrogen conditions has not been fully investigated. In this study, to evaluate the role of Arabidopsis G-proteins towards yield and nitrogen use efficiency (NUE), overexpressing transgenic lines AtGPA1, AtAGB1 together with AtAGG1 (AGB1-AGG1), with AtAGG2 (AGB1-AGG2), and with AtAGG3 (AGB1-AGG3) were created in the \"K407\" Brassica napus (B. napus). Analysis of multiple transgenic B. napus lines showed that overexpression of GPA1, AGB1-AGG1, AGB1-AGG2, or AGB1-AGG3 increased the biomass of seedling plants including a well-developed root system and increased nitrogen uptake under low and high nitrogen conditions. The activity of glutamine synthetase (GS), a key nitrogen assimilating enzyme, as well as the expression levels of genes that are involved in nitrogen uptake and assimilation were significantly increased in overexpressing plants under low nitrogen conditions. These properties enabled overexpressing plants to increase the number of seeds per silique by 12%-27% only under low nitrogen condition, effectively improving yield per plant by 9%-69% and NUE by 7%-49%. These results reveal roles of G-proteins in regulating seed traits and NUE, and provide a strategy that can substantially improve crop yield and NUE.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision Tuning of ABA Signaling by Ubiquitination of ABA Receptors: Modulating Protein Activity and Localization.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-19 DOI: 10.1093/jxb/eraf104

Chang Du, Zhonghui Zhang

引用次数: 0

White lupin: improving legume-based protein production via intercropping.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-19 DOI: 10.1093/jxb/eraf127

Corentin Dourmap, Joelle Fustec, Christophe Naudin, Nicolas Carton, Guillaume Tcherkez

{"title":"White lupin: improving legume-based protein production via intercropping.","authors":"Corentin Dourmap, Joelle Fustec, Christophe Naudin, Nicolas Carton, Guillaume Tcherkez","doi":"10.1093/jxb/eraf127","DOIUrl":"https://doi.org/10.1093/jxb/eraf127","url":null,"abstract":"Climate change, increased needs for food, industry and mitigation of environmental impacts are currently driving changes in agricultural practices. Moreover, increasing demand for plant-based protein in substitution to animal protein or to reduce soybean importations is driving cultivation of high-protein crops. Legumes are such crops that play a critical role in this process. Amongst them, white lupin is a so-called orphan species, i.e. associated with relatively little cultivated surface area worldwide and limited agronomic knowledge. Lupin is nevertheless very promising since seeds contain a high content of storage proteins with interesting nutritional properties. Also, it has low fertilisation requirements since it forms root clusters allowing efficient phosphorus (P) acquisition, along with symbiotic nitrogen (N) fixation by nodules. Nevertheless, lupin cultivation faces important challenges such as yield variability, slow vegetative development or susceptibility to weeds diseases and water stress, for example. Lupin has an enormous potential for resource-saving practices such as intercropping with non-legumes, because of niche complementarity for N acquisition and facilitation of P transfer to the associated species, which can in turn mitigate weeds and pests, and ensure yield stability. To overcome several bottlenecks associated with lupin cultivation (e.g. nutrient utilisation, drought resistance or limiting the impact of weeds), genetic, metabolic, and agronomic research is required in order to define ideotypes that are particularly well-fitted to sustainable agricultural practices such as intercropping, with optimal protein yield. This is one of the purposes of the trans-disciplinary research programme PULSAR, funded by France 2030, which aims to unlock several bottlenecks in lupin utilisation in agronomy.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and evolutionary evidence for drought adaptation of grass allopolyploid Brachypodium hybridum.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-19 DOI: 10.1093/jxb/eraf128

Yuanyuan Wang, Guang Chen, Fanrong Zeng, Fenglin Deng, Zujun Yang, Zhigang Han, Shengchun Xu, Eviatar Nevo, Pilar Catalán, Zhong-Hua Chen

{"title":"Genomic and evolutionary evidence for drought adaptation of grass allopolyploid Brachypodium hybridum.","authors":"Yuanyuan Wang, Guang Chen, Fanrong Zeng, Fenglin Deng, Zujun Yang, Zhigang Han, Shengchun Xu, Eviatar Nevo, Pilar Catalán, Zhong-Hua Chen","doi":"10.1093/jxb/eraf128","DOIUrl":"https://doi.org/10.1093/jxb/eraf128","url":null,"abstract":"Climate change increases the frequency and severity of drought worldwide, threatening the environmental resilience of cultivated grasses. However, the genetic diversity in many wild grasses could contribute to the development of climate-adapted varieties. Here, we elucidated the impact of polyploidy on drought response using allotetraploid Brachypodium hybridum (Bh) and its progenitor diploid species B. stacei (Bs). Our findings suggest that progenitor species' genomic legacies resulting from hybridization and whole-genome duplications conferred greater ecological adaptive advantages to Bh over Bs. Genes related to stomatal regulation and immune response from S-subgenomes were under positive selection during speciation, underscoring their evolutionary importance in adapting to environmental stresses. Biased expression in polyploid subgenomes [B. stacei-type (Bhs) and B. distachyon-type (Bhd)] significantly influenced differential gene expression, with the dominant subgenome exhibiting more differential expression. B. hybridum adapted a drought escape strategy characterized by higher photosynthetic capacity and lower WUEi than Bs, driven by a highly correlated co-expression network involving genes in the circadian rhythm pathway. In summary, our study showed the influence of polyploidy on ecological and environmental adaptation and resilience in model Brachypodium grasses. These insights hold promise for informing the breeding of climate-resilient cereal crops and pasture grasses.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Physiological Responses of Resistant and Susceptible Grape Cultivars to Eutypa dieback.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-18 DOI: 10.1093/jxb/eraf103

Gabriela C Sinclair, Renaud Travadon, Paula J Eschen, Christopher Wallis, Kendra Baumgartner, Chloé E L Delmas, Joshua F Hnizdor, Megan K Bartlett

{"title":"Differential Physiological Responses of Resistant and Susceptible Grape Cultivars to Eutypa dieback.","authors":"Gabriela C Sinclair, Renaud Travadon, Paula J Eschen, Christopher Wallis, Kendra Baumgartner, Chloé E L Delmas, Joshua F Hnizdor, Megan K Bartlett","doi":"10.1093/jxb/eraf103","DOIUrl":"https://doi.org/10.1093/jxb/eraf103","url":null,"abstract":"Eutypa lata is a fungal pathogen of grapevine that causes widespread economic damage and threatens vineyard longevity worldwide. This study was initiated to further understanding of how grapevines resist E. lata infections, using an integrated approach combining inoculation assays in the greenhouse with physiological and biochemical measurements. Resistant 'Zinfandel' and susceptible 'Syrah' grapevines were subjected to control and inoculation treatments and assessed for gas exchange, water status, photosynthetic biochemistry, hydraulic conductivity, wood chemistry, and fungal spread (lesion length). Infection reduced leaf photochemical function and gas exchange in Zinfandel and increased these variables in Syrah (p<0.05). Infection produced shorter lesions in Zinfandel (p<0.05), suggesting that downregulating gas exchange limited pathogen spread by reducing the carbon supply to the pathogen or fungal movement in the transpiration stream. Neither cultivar upregulated wood defense compounds in response to infection, but proanthocyanidin and catechin levels were constitutively higher in Zinfandel and stilbenoid and flavonoid contents were constitutively higher in Syrah (p<0.05). Altogether, this study is the first to show that, counterintuitively, downregulating physiological function in response to infection improves long-term resistance to E. lata. Screening responses in photochemical function or gas exchange could provide a high-throughput alternative to measuring lesion lengths in assessing resistance.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interspecies predictions of growth traits from quantitative transcriptome data acquired during fruit development.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-18 DOI: 10.1093/jxb/eraf122

Chloé Beaumont, Sylvain Prigent, Kentaro Mori, Pierre Baldet, Joana Jorly, Georges Randriafanomezantsoa-Radohery, Bertrand Beauvoit, Stéphanie Pateyron, Etienne Delannoy, Pierre Pétriacq, Yves Gibon, Sophie Colombié

{"title":"Interspecies predictions of growth traits from quantitative transcriptome data acquired during fruit development.","authors":"Chloé Beaumont, Sylvain Prigent, Kentaro Mori, Pierre Baldet, Joana Jorly, Georges Randriafanomezantsoa-Radohery, Bertrand Beauvoit, Stéphanie Pateyron, Etienne Delannoy, Pierre Pétriacq, Yves Gibon, Sophie Colombié","doi":"10.1093/jxb/eraf122","DOIUrl":"https://doi.org/10.1093/jxb/eraf122","url":null,"abstract":"Linking genotype and phenotype is a fundamental challenge in biology. In this respect, machine learning is playing a pivotal role in systems biology. As a central phenotypic trait, fruit development and its relative growth rate (RGR) result from interactions between gene regulation, metabolism and environment. In the present study, we carried out a multispecies transcriptomic analysis of nine different fruits. To illustrate fruit transcriptomes, transcripts were first compared using multivariate methods, revealing main similar profiles. They were then used as variables to predict four growth traits, i.e. RGR, developmental progress, fruit weight and protein content, using generalised linear models (GLMs) to decipher the mechanisms involving gene expression in development. The predictions were very satisfactory despite disparities when the model did not include the entire panel of fruit species. Based on orthogroups derived from BLAST and annotated consensus sequences from gene ontology (GO) terminology, variables annotated for metabolic processes, especially those involving cell wall carbohydrates and proteins, were found to be the most effective in predicting growth. In addition, predictions were improved for RGR when introducing a seven-day lag between transcript contents and growth traits, suggesting the necessity of considering the proteins produced to enhance phenotypic trait predictions. These original results showed that growth traits can be predicted very well with GLMs based on orthogroups from multi-species transcriptomes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0