Journal of Experimental Botany最新文献_第3页

Turgor pressure affects transverse stiffness and resonant frequencies of buzz-pollinated poricidal anthers.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-19 DOI: 10.1093/jxb/erae504

Mitchell Alvord, Jenna McNally, Cailin Casey, Mark Jankauski

{"title":"Turgor pressure affects transverse stiffness and resonant frequencies of buzz-pollinated poricidal anthers.","authors":"Mitchell Alvord, Jenna McNally, Cailin Casey, Mark Jankauski","doi":"10.1093/jxb/erae504","DOIUrl":"https://doi.org/10.1093/jxb/erae504","url":null,"abstract":"Several agriculturally valuable plants store their pollen in tube-like poricidal anthers, which release pollen through buzz pollination. In this process, bees rapidly vibrate the anther using their indirect flight muscles. The stiffness and resonant frequency of the anther are crucial for effective pollen release, yet the impact of turgor pressure on these properties is not well understood. Here, we perform three-point flexure tests and experimental modal analysis to determine anther transverse stiffness and resonant frequency, respectively. Dynamic nanoindentation is used to identify the anther's storage modulus as a function of excitation frequency. We subsequently develop mathematical models to estimate how turgor pressure changes after the anther is removed from a flower, thereby emulating zero water availability. We find that 30 minutes post-ablation, anther stiffness decreases by 60%. Anther resonant frequency decreases by 20% 60 minutes post-ablation. Models indicate turgor pressure in the fresh anther is about 0.2 - 0.3 MPa. Our findings suggest that natural fluctuations in turgor pressure due to environmental factors like temperature and light intensity may require bees to adjust their foraging behaviors. Interestingly, anther storage modulus increased with excitation frequency, underscoring the need for more sophisticated mechanical models that consider viscous fluid transport through plant tissue.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142854316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-omic profiles of Sorghum genotypes with contrasting heat tolerance connect pathways related to thermotolerance.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-19 DOI: 10.1093/jxb/erae506

Alexander Watson-Lazowski, Francisco Javier Cano, Mikael Kim, Urs Benning, Fiona Koller, Barbara George-Jaeggli, Alan Cruickshank, Emma Mace, David Jordan, Mathieu Pernice, Charles Warren, Oula Ghannoum

{"title":"Multi-omic profiles of Sorghum genotypes with contrasting heat tolerance connect pathways related to thermotolerance.","authors":"Alexander Watson-Lazowski, Francisco Javier Cano, Mikael Kim, Urs Benning, Fiona Koller, Barbara George-Jaeggli, Alan Cruickshank, Emma Mace, David Jordan, Mathieu Pernice, Charles Warren, Oula Ghannoum","doi":"10.1093/jxb/erae506","DOIUrl":"https://doi.org/10.1093/jxb/erae506","url":null,"abstract":"Understanding how crop varieties acclimate to elevated temperatures is key to priming them for future climates. Here, we exposed two genotypes of Sorghum bicolor (one sensitive to heat shock (Sen) and one tolerant (Tol)) from multiple growth temperatures to a six-day heat shock (reaching 45°C), carrying out a suite of measurements before and during heat shock. Sen consistently reduced photosynthetic functioning during heat shock, while Tol increased its photosynthetic rate. Higher abundance of heat shock protein transcripts and metabolites related to heat tolerance were noted for Tol when compared to Sen both before and during heat shock, which can be attributed to constitutive and inducible responses to elevated temperatures. In addition, important changes in metabolic pathways were clearly identified for Tol during heat shock (including upregulation of raffinose family oligosaccharides and downregulation of the GABA catalytic pathway), even as the concentration of hexose sugars became depleted. We infer Tol was able to tolerate elevated temperatures due to an upregulation of osmoprotectants, chaperones and reactive oxygen species scavengers and by the suppression of SnRK1 via transcripts and metabolites during heat shock. Our results highlight potential targets for attributes of high temperature tolerance which can be utilised in future breeding trials.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure. 玉米水蒸发蛋白 ZmPIP1;6 可增强气孔开放以及 CO2- 和 ABA 诱导的气孔关闭。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-19 DOI: 10.1093/jxb/erae500

Lei Ding, Maxime J Laurent, Thomas Milhiet, Stijn Aesaert, Mieke Van Lijsbettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, François Chaumont

{"title":"The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure.","authors":"Lei Ding, Maxime J Laurent, Thomas Milhiet, Stijn Aesaert, Mieke Van Lijsbettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, François Chaumont","doi":"10.1093/jxb/erae500","DOIUrl":"https://doi.org/10.1093/jxb/erae500","url":null,"abstract":"The plasma membrane aquaporin ZmPIP1;6 is expressed in maize stomatal complexes, with higher expression during the day than at night. To elucidate the role of ZmPIP1;6 in gas exchange and stomatal movement, it was expressed in maize (inbred line B104) under the control of p35S promoter (OE) or its native promoter fused with mYFP cDNA (mYFP-ZmPIP1;6). In stomatal complexes of the leaf mature zone, mYFP-ZmPIP1;6 showed higher expression in subsidiary cells than in guard cells, with light and dark treatments influencing its subcellular localization. Notably, ZmPIP1;6 internalization increased in dark conditions versus light. Stomatal opening was greater in ZmPIP1;6 OE than in wild type (WT), while closure exhibited greater sensitivity to elevated CO2 concentration or ABA treatment. Our finding revealed that reactive oxygen species (H2O2) was involved in ABA-induced stomatal closure, while ZmPIP1;6 was unable to facilitate H2O2 diffusion when expressed in yeast. Finally, ZmPIP1;6 OE and mYFP-ZmPIP1;6 transgenic plants exhibited higher abaxial stomatal density than WT. Overall, these results indicate that ZmPIP1;6 plays important roles in stomatal opening and CO2- and ABA-induced stomatal closure.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiPEPs and cPEPs as tools to monitor plant gene expression and develop alternative strategies in agriculture.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-17 DOI: 10.1093/jxb/erae501

Patrice Thuleau, Mélanie Ormancey, Serge Plaza, Jean-Philippe Combier

{"title":"MiPEPs and cPEPs as tools to monitor plant gene expression and develop alternative strategies in agriculture.","authors":"Patrice Thuleau, Mélanie Ormancey, Serge Plaza, Jean-Philippe Combier","doi":"10.1093/jxb/erae501","DOIUrl":"https://doi.org/10.1093/jxb/erae501","url":null,"abstract":"In order to develop a sustainable agriculture respecting the environment and to reduce chemical inputs, a new strategy has emerged in recent years, based on the use of products targeting plants' natural defense and growth mechanisms. In this context, a few years ago we demonstrated the existence in plants of regulatory peptides called miPEPs for \"microRNA-encoded peptides\". MicroRNAs (miRNAs) are small RNAs that down-regulate the expression of numerous genes in eukaryotes. MiPEPs increase the amount of their cognate miRNA, resulting in an even greater decrease in the expression of genes targeted by these miRNAs. Thus, the application of an artificial miPEP to a plant causes a phenotype associated with a change in the genes targeted by the corresponding miRNA. More recently we also identified new regulatory peptides, namely cPEPs for \"complementary peptides\", capable of enhancing the activity of a given protein, independently of any increase in transcriptional activity. Moreover, as for the miPEPs, the external application of these new peptides makes it possible to regulate the phenotypes associated with the targeted proteins. This review highlights the most recent advances in the potential use of miPEPs and cPEPs in agronomy to improve plant development and stress tolerance.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142846807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histidine limitation alters plant development and influences the TOR network. 组氨酸限制改变了植物的发育并影响了 TOR 网络。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-17 DOI: 10.1093/jxb/erae479

Amandine Guérin, Caroline Levasseur, Aline Herger, Dominik Renggli, Alexandros Georgios Sotiropoulos, Gabor Kadler, Xiaoyu Hou, Myriam Schaufelberger, Christian Meyer, Thomas Wicker, Laurent Bigler, Christoph Ringli

{"title":"Histidine limitation alters plant development and influences the TOR network.","authors":"Amandine Guérin, Caroline Levasseur, Aline Herger, Dominik Renggli, Alexandros Georgios Sotiropoulos, Gabor Kadler, Xiaoyu Hou, Myriam Schaufelberger, Christian Meyer, Thomas Wicker, Laurent Bigler, Christoph Ringli","doi":"10.1093/jxb/erae479","DOIUrl":"https://doi.org/10.1093/jxb/erae479","url":null,"abstract":"Plant growth depends on growth regulators, nutrient availability, and amino acids levels, all of which influence cell wall formation and cell expansion. Cell wall integrity and structures are surveyed and modified by a complex array of cell wall integrity sensors, including LRR-extensins (LRXs) that bind RALF (rapid alkalinization factor) peptides with high affinity and help to compact cell walls. Expressing the Arabidopsis root-hair specific LRX1 without the extensin domain, which anchors the protein to the cell wall, has a negative effect on root hair development. The mechanism of this negative effect was investigated by a suppressor screen, which led to the identification of a sune (suppressor of dominant-negative LRX1) mutant collection. The sune82 mutant was identified as an allele of HISN2, which encodes an enzyme essential for histidine biosynthesis. This mutation leads to reduced accumulation of histidine and an increase in several amino acids, which appears to have an effect on the TOR (target of rapamycin) network, a major controller of eukaryotic cell growth. It also represents an excellent tool to study the effects of reduced histidine levels on plant development, as it is a rare example of a viable partial loss-of-function allele in an essential biosynthetic pathway.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intrinsic cues guiding changes in division orientation in the Arabidopsis root meristem - a formative experience. 拟南芥根分生组织分裂方向变化的内在线索--一种形成经验。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-17 DOI: 10.1093/jxb/erae509

Nataliia Konstantinova, Claudia von der Mark, Bert De Rybel

引用次数: 0

Time-resolved transcriptomic of single V. vinifera fruits: membrane transports as switches of the double sigmoidal growth. 单个葡萄果实的时间分辨转录组学：膜运输是双西格玛生长的开关。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-16 DOI: 10.1093/jxb/erae502

Stefania Savoi, Mengyao Shi, Gautier Sarah, Audrey Weber, Laurent Torregrosa, Charles Romieu

{"title":"Time-resolved transcriptomic of single V. vinifera fruits: membrane transports as switches of the double sigmoidal growth.","authors":"Stefania Savoi, Mengyao Shi, Gautier Sarah, Audrey Weber, Laurent Torregrosa, Charles Romieu","doi":"10.1093/jxb/erae502","DOIUrl":"https://doi.org/10.1093/jxb/erae502","url":null,"abstract":"By revealing that the grape berry loses one H+ per accumulated sucrose at the inception of ripening, adopting a single fruit paradigm elucidates the fundamentals of the malate-sugar nexus, previously obscured by asynchrony in population-based models of ripening. More broadly, the development of the individual fruit was revisited from scratch to capture the simultaneous changes in gene expression and metabolic fluxes in a kinetically relevant way from flowering to overripening. Dynamics in water, tartrate, malate, hexoses, and K+ fluxes obtained by combining individual single fruit growth and concentration data allowed to define eleven sub-phases in fruit development, which distributed on a rigorous curve in RNAseq PCA. WGCNA achieved unprecedented time resolutions in exploring transcript level-metabolic rate associations. A comprehensive set of membrane transporters was found specifically expressed during the first growth phase related to vacuolar over-acidification. Unlike in slightly more acidic citrus, H+ V-PPase transcripts were predominantly expressed, followed by V-ATPase, clarifying the thermodynamic limit beyond which their replacement by the tonoplast P3A/P3B ATPase (PH5/PH1) complex turns compulsory. Puzzlingly, bona fide aluminum-activated malate transporter (ALMT) kept a low profile at this stage, possibly replaced by a predominating uncharacterized anion channel. At the onset of ripening, the switch role of hexose transporter HT6 in sugar accumulation is confirmed, electroneutralized by malate vacuolar leakage and H+ pumps activation.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A homeodomain leucine zipper protein RhHB22 promotes petal senescence by repressing ascorbic acid biosynthesis in rose. 同源结构域亮氨酸拉链蛋白 RhHB22 通过抑制玫瑰中抗坏血酸的生物合成促进花瓣衰老。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-16 DOI: 10.1093/jxb/erae503

Ping Li, Yuexuan Qiu, Rui Wang, Bingjie Zhang, Yanxing Ma, Xiaoming Sun, Junping Gao, Yunhe Jiang

{"title":"A homeodomain leucine zipper protein RhHB22 promotes petal senescence by repressing ascorbic acid biosynthesis in rose.","authors":"Ping Li, Yuexuan Qiu, Rui Wang, Bingjie Zhang, Yanxing Ma, Xiaoming Sun, Junping Gao, Yunhe Jiang","doi":"10.1093/jxb/erae503","DOIUrl":"https://doi.org/10.1093/jxb/erae503","url":null,"abstract":"Premature petal senescence dramatically reduces flower quality and value. Ethylene and reactive oxygen species (ROS) are key players in accelerating rose petal senescence, but the molecular mechanism by which ethylene antagonizes ROS scavenging is not well understood. Here, we show that ethylene reduces ascorbic acid (AsA) production, leading to the accumulation of ROS and hastening petal senescence. Ethylene treatment suppresses the expression of GDP-L-galactose phosphorylase 1 (RhGGP1), encoding the rate-controlling enzyme in AsA biosynthesis. A HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP) II transcriptional factor, RhHB22, directly binds to the promoter of RhGGP1 and inhibits its transcription. RhHB22 is induced by ethylene, and silencing of RhHB22 increases RhGGP1 expression and AsA production, resulting in reduced H2O2 accumulation and delayed petal senescence. Additionally, the delayed petal senescence symptoms of RhHB22-silenced plants were suppressed by silencing RhGGP1. Moreover, the expression of RhGGP1, which is suppressed by ethylene in wild-type petals, is significantly compromised in RhHB22-silenced petals. These findings uncover the transcriptional regulatory mechanism by which ethylene promotes ROS accumulation and petal senescence by inhibiting AsA biosynthesis, enhance our understanding of ethylene-induced petal senescence, and provide novel insights for improving the longevity of cut flowers.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Virulence Mechanism of Leptosphaeria maculans in the Brassica napus Interaction: The Key Role of Sirodesmin PL in Cell Death Induction. 揭示Leptosphaeria maculans在甘蓝菜相互作用中的致病机制：Sirodesmin PL 在诱导细胞死亡中的关键作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-16 DOI: 10.1093/jxb/erae498

Marina A Pombo, Hernan G Rosli, Santiago Maiale, Candace Elliott, Micaela E Stieben, Fernando M Romero, Andrés Garriz, Oscar A Ruiz, Alexander Idnurm, Franco R Rossi

{"title":"Unveiling the Virulence Mechanism of Leptosphaeria maculans in the Brassica napus Interaction: The Key Role of Sirodesmin PL in Cell Death Induction.","authors":"Marina A Pombo, Hernan G Rosli, Santiago Maiale, Candace Elliott, Micaela E Stieben, Fernando M Romero, Andrés Garriz, Oscar A Ruiz, Alexander Idnurm, Franco R Rossi","doi":"10.1093/jxb/erae498","DOIUrl":"https://doi.org/10.1093/jxb/erae498","url":null,"abstract":"Leptosphaeria maculans is the causal agent of blackleg disease in Brassica napus, leading to substantial yield losses. Sirodesmin PL, the principal toxin produced by L. maculans, has been implicated in the infective process in plants. However, the precise molecular and physiological mechanisms governing its effects remain elusive. This study investigates the changes induced by Sirodesmin PL at the transcriptomic, physiological, and morphological levels in B. napus cotyledons. Sirodesmin PL treatment upregulates genes associated with plant defense processes, including response to chitin, sulfur compound biosynthesis, toxin metabolism, oxidative stress response, and jasmonic acid/ethylene synthesis and signaling. Validation of these transcriptomic changes is evidenced by several typical defense response processes, such as the accumulation of reactive oxygen species (ROS) and callose deposition. Concomitantly, oxidized Sirodesmin PL induces concentration- and exposure duration-dependent cell death. This cellular death is likely attributed to diminished activity of photosystem II and a reduction in the number of chloroplasts per cell. In agreement, a down-regulation of genes associated with the photosynthesis process is observed following Sirodesmin PL treatment. Thus, it is plausible that L. maculans exploits Sirodesmin PL as a virulence factor to instigate cell death in B. napus during its necrotrophic stage, favoring the infective process.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The selective estrogen receptor modulator clomiphene inhibits sterol biosynthesis in Arabidopsis thaliana. 选择性雌激素受体调节剂氯米芬抑制拟南芥固醇的生物合成

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-16 DOI: 10.1093/jxb/erae481

Qing Wang, Kjell De Vriese, Sandrien Desmet, Ren Wang, Markéta Luklová, Qianqian Liu, Jacob Pollier, Qing Lu, Sarah Schlag, Walter Vetter, Alain Goossens, Eugenia Russinova, Geert Goeminne, Danny Geelen, Tom Beeckman, Steffen Vanneste

{"title":"The selective estrogen receptor modulator clomiphene inhibits sterol biosynthesis in Arabidopsis thaliana.","authors":"Qing Wang, Kjell De Vriese, Sandrien Desmet, Ren Wang, Markéta Luklová, Qianqian Liu, Jacob Pollier, Qing Lu, Sarah Schlag, Walter Vetter, Alain Goossens, Eugenia Russinova, Geert Goeminne, Danny Geelen, Tom Beeckman, Steffen Vanneste","doi":"10.1093/jxb/erae481","DOIUrl":"https://doi.org/10.1093/jxb/erae481","url":null,"abstract":"Sterols are produced via complex, multistep biosynthetic pathways involving similar enzymatic conversions in plants, animals and fungi, yielding a variety of sterol metabolites with slightly different chemical properties to exert diverse and specific functions. A tremendously diverse landscape of sterols, and sterol-derived compounds, can be found across the plant kingdom, determining a wide spectrum of functions. Resolving the underlying biosynthetic pathways is thus instrumental to understanding the function and use of these molecules. In only a few plants, sterol biosynthesis has been studied using mutants. In non-model species a pharmacological approach is required. However, this relies on only a few inhibitors. Here, we probed a collection of inhibitors of mammalian cholesterol biosynthesis to identify new inhibitors of plant sterol biosynthesis. We show that imidazole-type fungicides, bifonazole, clotrimazole and econazole inhibit the obtusifoliol 14α-demethylase CYP51 in plants. Moreover, we found that the selective estrogen receptor modulator, clomiphene, inhibits sterol biosynthesis in part by inhibiting the plant-specific cyclopropyl-cycloisomerase CPI1. These results demonstrate that rescreening of inhibitors animal sterol biosynthesis is an easy approach for identifying novel inhibitors of plant sterol biosynthesis. These molecules expand the toolkit for studying and manipulating sterol biosynthesis in the plant kingdom.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0