Journal of Experimental Botany最新文献_第3页

Nitrogen demand and availability: relative roles in driving C3 plant responses to elevated CO2. 氮需求和有效性：驱动C3植物对CO2升高响应的相对作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf149

Xuan Hu, Mirindi Eric Dusenge

引用次数: 0

Enhanced ureide partitioning improves soybean performance under drought stress. 加强尿素分配可提高干旱胁迫下大豆的生产性能。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf099

Sandi Win Thu, Mechthild Tegeder

{"title":"Enhanced ureide partitioning improves soybean performance under drought stress.","authors":"Sandi Win Thu, Mechthild Tegeder","doi":"10.1093/jxb/eraf099","DOIUrl":"10.1093/jxb/eraf099","url":null,"abstract":"Soybean [Glycine max (L.) Merr.] fixes atmospheric nitrogen through a symbiotic relationship with rhizobia in root nodules to produce allantoin and allantoic acid. These ureides serve as primary nitrogen transport compounds that are moved from nodules to shoots in support of physiological functions and organ growth. Nodule ureide permease 1 (UPS1) is important for this transport process. Drought stress inhibits nitrogen fixation and reduces productivity in soybean, which has been associated with the accumulation of ureides in both nodule and shoot tissues. In this study, it was hypothesized that changes in ureide nodule-to-leaf-to-sink partitioning through manipulation of UPS1 function would alter ureide tissue concentrations, ultimately influencing soybean responses to drought. Soybean plants overexpressing UPS1 were exposed to moderate and severe drought conditions. Changes in organ and phloem ureide concentrations indicated enhanced nodule-to-shoot ureide transport and increased sink nitrogen supply in the transgenic plants compared with control wild-type plants. We further uncovered improvements in carbon fixation, partitioning, and availability for nitrogen fixation, resulting in increased nitrogen gains and better growth of the drought-stressed UPS1-overexpressing lines. Overall, our findings demonstrate that enhanced ureide partitioning contributes to improved soybean performance not only under well-watered conditions but also under drought stress.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2786-2808"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana. 拟南芥根细胞的液泡结构是由网格蛋白介导的运输驱动的。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf084

Claudio Osorio-Navarro, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, Lorena Norambuena

{"title":"The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana.","authors":"Claudio Osorio-Navarro, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, Lorena Norambuena","doi":"10.1093/jxb/eraf084","DOIUrl":"10.1093/jxb/eraf084","url":null,"abstract":"The central vacuole is a multifunctional organelle with the most significant occupancy in a differentiated plant cell. Plants depend on the function of the vacuole for critical development, growth, and environmental responses. As the cell expands, the vacuole changes shape and size, increasing its membrane and luminal content. The set of these events is called the vacuolar configuration process, which has not been well described. Our research highlights the impact of plasma membrane internalization on vacuole morphology during the vacuolar configuration process. We observed a direct correlation between differential endocytosis rates and the enrichment of vacuolar membranous structures. Chemical and genetic interference with clathrin-mediated endocytosis (CME) revealed that it is required for the vacuolar configuration of growing root cells. The contribution of CME to the vacuole configuration process co-occurs with the induction of post-trans-Golgi network (TGN)/early endosome (EE) trafficking with the participation of the Rab GTPases ARA6 and ARA7. Our results show that the CME plays an active role during vacuole configuration, most probably carrying the material that allows the establishment of the vacuole in elongating cells. Since membrane trafficking through the EE/TGN is required to reach the vacuole, additional players must be defined.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2700-2714"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen demand, availability, and acquisition strategy control plant responses to elevated CO2. 氮需求、可用性和获取策略控制植物对CO2升高的响应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf118

Evan A Perkowski, Ezinwanne Ezekannagha, Nicholas G Smith

{"title":"Nitrogen demand, availability, and acquisition strategy control plant responses to elevated CO2.","authors":"Evan A Perkowski, Ezinwanne Ezekannagha, Nicholas G Smith","doi":"10.1093/jxb/eraf118","DOIUrl":"10.1093/jxb/eraf118","url":null,"abstract":"Plants respond to increasing atmospheric CO2 concentrations by reducing leaf nitrogen content and photosynthetic capacity-patterns that correspond with increased net photosynthesis and growth. Despite the longstanding notion that nitrogen availability regulates these responses, eco-evolutionary optimality theory posits that leaf-level responses to elevated CO2 are driven by leaf nitrogen demand for building and maintaining photosynthetic enzymes and are independent of nitrogen availability. In this study, we examined leaf and whole-plant responses of Glycine max L. (Merr) subjected to full-factorial combinations of two CO2, two inoculation, and nine nitrogen fertilization treatments. Nitrogen fertilization and inoculation did not alter leaf photosynthetic responses to elevated CO2. Instead, elevated CO2 decreased the maximum rate of ribulose-1,5-bisophosphate oxygenase/carboxylase (Rubisco) carboxylation more strongly than it decreased the maximum rate of electron transport for ribulose-1,5-bisphosphate (RuBP) regeneration, increasing net photosynthesis by allowing rate-limiting steps to approach optimal coordination. Increasing fertilization enhanced positive whole-plant responses to elevated CO2 due to increased below-ground carbon allocation and nitrogen uptake. Inoculation with nitrogen-fixing bacteria did not influence plant responses to elevated CO2. These results reconcile the role of nitrogen availability in plant responses to elevated CO2, showing that leaf photosynthetic responses are regulated by leaf nitrogen demand while whole-plant responses are constrained by nitrogen availability.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2908-2923"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

White lupin: improving legume-based protein production via intercropping. 白豆：通过间作提高豆科植物蛋白质产量。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf127

Corentin Dourmap, Joelle Fustec, Christophe Naudin, Nicolas Carton, Guillaume Tcherkez

{"title":"White lupin: improving legume-based protein production via intercropping.","authors":"Corentin Dourmap, Joelle Fustec, Christophe Naudin, Nicolas Carton, Guillaume Tcherkez","doi":"10.1093/jxb/eraf127","DOIUrl":"10.1093/jxb/eraf127","url":null,"abstract":"Climate change, increased demand for food, industry, and mitigation of environmental impacts are currently driving changes in agricultural practice. Moreover, increasing demand for plant-based protein as substitutes for animal protein or to reduce soybean importations is driving cultivation of high-protein crops. Legumes crops play a critical role in this process. Amongst them is white lupin (Lupinus albus), a so-called orphan species, meaning it has relatively little cultivated surface area worldwide and limited agronomic knowledge. Lupin is, nevertheless, very promising since its seeds have a high content of storage proteins with interesting nutritional properties. It also has low fertilisation requirements since it forms root clusters allowing efficient phosphorus (P) acquisition, along with symbiotic nitrogen (N) fixation by nodules. Nevertheless, lupin cultivation faces important challenges such as yield variability, slow vegetative development, susceptibility to weeds diseases, and water stress. Lupin has an enormous potential for resource-saving practices such as intercropping with non-legumes, because of niche complementarity for N acquisition and facilitation of P transfer to the associated species, which can in turn mitigate weeds and pests, and ensure yield stability. To overcome several bottlenecks associated with lupin cultivation (e.g. nutrient utilisation, drought resistance or limiting the impact of weeds), genetic, metabolic, and agronomic research is required in order to define ideotypes that are particularly well-fitted to sustainable agricultural practices such as intercropping, with optimal protein yield. This is one of the purposes of the trans-disciplinary research programme PULSAR, funded by France 2030, which aims to unlock several bottlenecks in lupin utilisation in agronomy.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2673-2687"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of cytochrome P450 CYP72A1182 in metabolic herbicide resistance evolution in Amaranthus palmeri populations. 细胞色素P450 CYP72A1182在苋属植物代谢性抗除草剂进化中的作用

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf114

Carlos Alberto Gonsiorkiewicz Rigon, Anita Küpper, Crystal Sparks, Jacob Montgomery, Falco Peter, Simon Schepp, Alejandro Perez-Jones, Patrick J Tranel, Roland Beffa, Franck E Dayan, Todd A Gaines

{"title":"Function of cytochrome P450 CYP72A1182 in metabolic herbicide resistance evolution in Amaranthus palmeri populations.","authors":"Carlos Alberto Gonsiorkiewicz Rigon, Anita Küpper, Crystal Sparks, Jacob Montgomery, Falco Peter, Simon Schepp, Alejandro Perez-Jones, Patrick J Tranel, Roland Beffa, Franck E Dayan, Todd A Gaines","doi":"10.1093/jxb/eraf114","DOIUrl":"10.1093/jxb/eraf114","url":null,"abstract":"Evolution of metabolic herbicide resistance is a major issue for weed management. Few genes and regulatory mechanisms have been identified, particularly in dicotyledonous weed species. We identified putative causal genes and regulatory mechanism for tembotrione resistance in Palmer amaranth (Amaranthus palmeri). Cytochrome P450 candidate genes were identified through RNA-seq analysis. We validated their functions using heterologous expression in Saccharomyces cerevisae. Promoters of the candidate P450 genes were analysed. We performed QTL mapping to identify genomic regions associated with resistance. CYP72A1182 metabolized tembotrione in a heterologous system. The CYP72A1182 gene had increased expression in other A. palmeri populations resistant to multiple herbicides, including tembotrione. Resistant plants exhibited polymorphisms in the promoter of CYP72A1182. We identified quantitative trait loci linked to herbicide resistance, including one on chromosome 4 approximately 3 Mb away from CYP72A1182. CYP72A1182 is likely involved in tembotrione resistance in A. palmeri. Increased expression of this gene could be due to cis-regulation in the promoter, as well as trans-regulation from transcription factors, and further studies are in progress to test this hypothesis. The elucidation of regulatory genes is crucial for developing innovative weed management approaches and target-based novel herbicide molecules.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2891-2907"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12223498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Onwards and upwards. 向前，向上。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf126

John E Lunn

引用次数: 0

Retraction of: Interactions between nitrate reductase 2 and catalase 1 fine-tune disease resistance in cassava. 撤回：硝酸还原酶2和过氧化氢酶1的相互作用微调木薯的抗病性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf077

引用次数: 0

The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure. 玉米水蒸发蛋白 ZmPIP1;6 可增强气孔开放以及 CO2- 和 ABA 诱导的气孔关闭。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/erae500

Lei Ding, Maxime J Laurent, Thomas Milhiet, Stijn Aesaert, Mieke Van Lijsebettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, François Chaumont

{"title":"The maize aquaporin ZmPIP1;6 enhances stomatal opening and CO2- and ABA-induced stomatal closure.","authors":"Lei Ding, Maxime J Laurent, Thomas Milhiet, Stijn Aesaert, Mieke Van Lijsebettens, Laurens Pauwels, Hilde Nelissen, Dirk Inzé, François Chaumont","doi":"10.1093/jxb/erae500","DOIUrl":"10.1093/jxb/erae500","url":null,"abstract":"The plasma membrane aquaporin ZmPIP1;6 is expressed in maize stomatal complexes, with higher expression during the day than at night. To elucidate the role of ZmPIP1;6 in gas exchange and stomatal movement, it was expressed in maize (inbred line B104) under the control of the p35S promoter (overexpression) or its native promoter fused with mYFP (monomeric yellow fluorescent protein) cDNA (mYFP-ZmPIP1;6). In stomatal complexes of the mature zone of the leaf, mYFP-ZmPIP1;6 showed higher expression in subsidiary cells than in guard cells, and light and dark treatments influenced its subcellular localization. Notably, ZmPIP1;6 internalization increased in dark conditions compared with light. Stomatal opening was greater in ZmPIP1;6 overexpression plants than in the wild type, while closure exhibited greater sensitivity to elevated CO2 concentration or abscisic acid (ABA) treatment. Our findings revealed that reactive oxygen species (H2O2) were involved in ABA-induced stomatal closure, while ZmPIP1;6 was unable to facilitate H2O2 diffusion when expressed in yeast. Finally, ZmPIP1;6 overexpression and mYFP-ZmPIP1;6 transgenic plants exhibited higher abaxial stomatal density than the wild type. Overall, these results indicate that ZmPIP1;6 plays important roles in stomatal opening and CO2- and ABA-induced stomatal closure.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2832-2845"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12223514/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming physiological trade-offs between flowering time and crop yield: strategies for a changing climate. 克服开花时间和作物产量之间的生理平衡——应对气候变化的策略。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-07-02 DOI: 10.1093/jxb/eraf110

Astrid Wingler, Soualihou Soualiou

{"title":"Overcoming physiological trade-offs between flowering time and crop yield: strategies for a changing climate.","authors":"Astrid Wingler, Soualihou Soualiou","doi":"10.1093/jxb/eraf110","DOIUrl":"10.1093/jxb/eraf110","url":null,"abstract":"Early flowering of annual plants can lead to resource limitation owing to reduced uptake of nitrogen during the reproductive phase and declining foliar photosynthesis as a result of monocarpic senescence. Low availability of accumulated resources can therefore result in a trade-off between early flowering and reproductive fitness. However, green inflorescence organs (such as siliques, pods, bracts, or awns) can make considerable contributions to photosynthetic carbon gain, and in some cases provide more carbon to seed formation than the leaves. Inflorescence photosynthesis may thereby overcome the flowering time trade-off. In addition to providing photosynthates, inflorescence organs can contribute to seed nitrogen through senescence-dependent nitrogen recycling. In annual crops, breeding has resulted in increased carbon allocation to the grain and higher harvest index, but in some cases this had led to reduced grain protein content. We discuss different breeding targets to address carbon and nitrogen limitation, dependent on the climatic environment. For environments that are prone to drought, we propose a combination of early flowering with enhanced inflorescence photosynthesis or, alternatively, delayed senescence (stay-green) associated with improved water balance. For optimized yield and grain protein content under favourable conditions, enhanced sink strength and extended nitrogen uptake are suggested as breeding targets.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2646-2658"},"PeriodicalIF":5.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12223507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0