Journal of Experimental Botany最新文献_第3页

A dual role for a member of the WRYK family in regulating both cambial cell division and secondary cell wall formation in Populus. WRYK家族成员在杨树形成层细胞分裂和次生细胞壁形成中的双重作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-10 DOI: 10.1093/jxb/eraf259

Shuyi Li, Na Xu, Xiaoqi Xu, Zhen Tian, Xingkai Sun, Bowen Zhou, Li-Jun Liu

{"title":"A dual role for a member of the WRYK family in regulating both cambial cell division and secondary cell wall formation in Populus.","authors":"Shuyi Li, Na Xu, Xiaoqi Xu, Zhen Tian, Xingkai Sun, Bowen Zhou, Li-Jun Liu","doi":"10.1093/jxb/eraf259","DOIUrl":"https://doi.org/10.1093/jxb/eraf259","url":null,"abstract":"WRKY transcription factors are unique to plants and play diverse roles in plants development and responses to biotic/abiotic stresses. In this study, we functionally characterized the WRKY transcription factor PagWRKY39a as a repressor of stem secondary growth in Populus. PagWRKY39a expression level is significantly higher in secondary phloem and cambium compared to early expanding secondary xylem. Compared to wild type (WT), overexpression of PagWRKY39a (PagWRKY39a-OE) significantly decreased stem diameter and plant height while knockout of PagWRKY39a (PagWRKY39a-KO) are not significantly different from WT. Further analysis showed that the vascular cambium cell layers, secondary xylem width, and secondary cell wall (SCW) thickness were significantly reduced in PagWRKY39a-OE but significantly increased in PagWRKY39a-KO. Transcriptome analysis revealed that genes related to mitotic cell cycle and SCW biosynthesis are significantly down-regulated in PagWRKY39a-OE plants while no significant changes in PagWRKY39a-KO plants. We also performed DNA affinity purification followed by sequencing (DAP-seq) assays, in coupled with yeast one-hybrid and expression analysis, to identify PagWRKY39a direct target genes during secondary growth. Our results showed that PagHB4, a key regulatory gene of secondary growth, was directly bound and repressed by PagWRKY39a. Together, our results demonstrated that PagWRKY39a plays an important role in regulating cambium activity and secondary xylem differentiation in Populus, which may have a significant effect on plant biomass production.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of cuticle, sterols, sphingolipids, and glycerolipids in plant defense. 角质层、固醇、鞘脂和甘油脂在植物防御中的作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-09 DOI: 10.1093/jxb/eraf255

Huazhen Liu, Mohammad Fazel Soltani Gishini, Tatsushi Kurokawa, Rahul Mohan Singh, Aardra Kachroo, Pradeep Kachroo

{"title":"Role of cuticle, sterols, sphingolipids, and glycerolipids in plant defense.","authors":"Huazhen Liu, Mohammad Fazel Soltani Gishini, Tatsushi Kurokawa, Rahul Mohan Singh, Aardra Kachroo, Pradeep Kachroo","doi":"10.1093/jxb/eraf255","DOIUrl":"https://doi.org/10.1093/jxb/eraf255","url":null,"abstract":"Lipids have long been recognized as essential components of plant cells, providing structural integrity, and serving as reservoirs of metabolic energy. However, the last decade has transformed our understanding of lipids, revealing their critical and dynamic roles in plant immunity. Far from being passive molecules, lipids and lipid-derived signals orchestrate diverse immune responses, bridging structural defense mechanisms with complex biochemical signaling networks. In this review, we delve into the roles of the cuticle, sterols, sphingolipids, and glycerolipids in plant defense. We explore how these lipid classes work individually and synergistically to fortify local barriers and facilitate systemic immune responses. We aim to illuminate the interconnectedness of these lipid types, identify emerging trends, and propose future directions to uncover their full potential in plant immunity. This focused perspective underscores the transformative impact of lipids on our understanding of plant-pathogen interactions and highlights their promise in improving crop disease resistance.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pre-disturbance plant condition drives intraspecific resprouting variability in Mediterranean shrubs. 干扰前植物条件驱动地中海灌木种内繁殖变异。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-09 DOI: 10.1093/jxb/eraf246

Maya A Zomer, Bruno Moreira, Juli G Pausas

{"title":"Pre-disturbance plant condition drives intraspecific resprouting variability in Mediterranean shrubs.","authors":"Maya A Zomer, Bruno Moreira, Juli G Pausas","doi":"10.1093/jxb/eraf246","DOIUrl":"https://doi.org/10.1093/jxb/eraf246","url":null,"abstract":"Many Mediterranean plants persist after disturbances by resprouting from dormant buds, and understanding factors driving variability in resprouting is crucial for predicting regeneration under global change. We hypothesized resprouting is influenced more by long-term environmental factors (e.g., historical aridity, fire history, soil nutrients) that shape pre-disturbance plant condition than by immediate water availability or local adaptations. We applied an experimental disturbance of two consecutive cuts in a short interval (1) in the field, targeting adult Anthyllis cytisoides and Globularia alypum plants along an environmental gradient (in Spain), and (2) in a common garden, using A. cytisoides grown from seeds of contrasting aridity provenance and subjected to contrasted water treatments. Intraspecific variability in resprouting predominantly occurred within populations, and was primarily determined by pre-disturbance plant size, underscoring the importance of individual history. Secondarily, aridity had a positive effect on resprouting, while higher fire frequency and the second cut had negative effects. We found no evidence of local adaptations, but young plants in the garden were dependent on water availability. Adult plants may be resilient to disturbance under low water availability but sensitive to increased disturbance frequency, while young plants may be vulnerable in a drier world, emphasizing ontogenetic differences in resprouting vulnerabilities.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The catabolism of branched-chain amino acids and tyrosine has a low contribution to the mitochondrial metabolism during senescence in Brassica napus L. 甘蓝型油菜衰老过程中支链氨基酸和酪氨酸的分解代谢对线粒体代谢的贡献较小。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-09 DOI: 10.1093/jxb/eraf212

Mathieu Aubert, Vanessa Clouet, Florian Guilbaud, Alain Bouchereau, Younès Dellero

{"title":"The catabolism of branched-chain amino acids and tyrosine has a low contribution to the mitochondrial metabolism during senescence in Brassica napus L.","authors":"Mathieu Aubert, Vanessa Clouet, Florian Guilbaud, Alain Bouchereau, Younès Dellero","doi":"10.1093/jxb/eraf212","DOIUrl":"https://doi.org/10.1093/jxb/eraf212","url":null,"abstract":"Winter oilseed rape (WOSR) is characterized by a low nitrogen remobilisation efficiency during developmental leaf senescence (DLS) compared to cereal crops. The catabolism of branched-chain amino acids (BCAA) and tyrosine plays a significant role in the recycling of protein-bound nitrogen and the fuelling of mitochondrial respiration during stress-induced senescence. In this study, we elucidated the role of BCAA and tyrosine catabolism during DLS in WOSR. To this end, we employed a multi-faceted approach, integrating transcriptional fingerprints, mitochondrial respiration measurements and 13C-labelling experiments during both DLS and dark-induced senescence (DIS). In general, the transcriptional regulation of BCAA and tyrosine catabolism was weakly correlated with apparent catabolic fluxes during DLS and DIS in WOSR. The absolute quantification and 13C-analysis of organic and amino acids by GC-MS showed that the catabolism of BCAA and tyrosine had a comparable contribution to the functioning of the tricarboxylic acid (TCA) cycle during DLS and DIS in WOSR. Isotopologue analysis of TCA cycle intermediates provided evidence that BCAA and tyrosine catabolism contribute minimally to the functioning of the TCA cycle during senescence. The role of BCAA and tyrosine catabolism could be essentially devoted to the recycling of nitrogen during senescence.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted controls on auxin metabolism during cellular reprogramming and organ regeneration in plants. 植物细胞重编程和器官再生过程中生长素代谢的多方面调控。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-09 DOI: 10.1093/jxb/eraf251

Kaisei Maruyama, Momoko Ikeuchi

引用次数: 0

Modern elite winter wheat cultivars use two physiological pathways to achieve yield stability. 现代优质冬小麦品种通过两种生理途径实现产量稳定。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-07 DOI: 10.1093/jxb/eraf191

Tien-Cheng Wang, Li-Yu Liu, Kirsten Weiß, Tsu-Wei Chen

{"title":"Modern elite winter wheat cultivars use two physiological pathways to achieve yield stability.","authors":"Tien-Cheng Wang, Li-Yu Liu, Kirsten Weiß, Tsu-Wei Chen","doi":"10.1093/jxb/eraf191","DOIUrl":"https://doi.org/10.1093/jxb/eraf191","url":null,"abstract":"Identifying target traits for breeding stable, high-yielding winter wheat cultivars is challenging due to the intricate interplays of genotype, environment and management practices. We hypothesized that yield stability could be achieved through multiple genotypic strategies and that agronomic management stimulating these strategies would enhance stability. To test this, three-years of field experiments were conducted using eight high-yielding elite cultivars and three agronomic practices:1) nitrogen levels (220 or 176 kg N/ha), 2) fertilizer application timing, and 3) two sowing dates. Detailed field phenotyping of 130 agronomic, phenological, chemical and physiological traits, resulted in 40,557 measured or derived trait values. Correlation and multivariate analyses suggested that management practices promoting grain number increased yield stability, while nitrogen level influenced the importance of application time and sowing date. Interestingly, modern elite cultivars exhibit two distinct physiological strategies coupling different source capacity and sink demand strategies to achieve genotypic yield stability: (1) coupling high tiller and grain numbers with longer canopy stay-green and higher carbon reserves and (2) coupling high grain length with low tiller number and greater remobilization of pre-anthesis carbon reserves. The integration of multiple physiological pathways could therefore facilitate the identification of trait combinations for yield stability breeding.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of biochemistry and anatomy for underwater carbon uptake in leaves of the amphibious plant Hygrophila difformis. 水陆两栖植物异湿藻叶片水下碳吸收的生物化学和解剖学特征。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-06 DOI: 10.1093/jxb/eraf250

Genki Horiguchi, Yusuke Mizokami, Naoki Hirotsu, Ko Noguchi

{"title":"Characterization of biochemistry and anatomy for underwater carbon uptake in leaves of the amphibious plant Hygrophila difformis.","authors":"Genki Horiguchi, Yusuke Mizokami, Naoki Hirotsu, Ko Noguchi","doi":"10.1093/jxb/eraf250","DOIUrl":"https://doi.org/10.1093/jxb/eraf250","url":null,"abstract":"Hygrophila difformis, a heterophyllous amphibious plant, shows photosynthetic acclimation of leaves developed under not only submerged (submerged leaves) but also terrestrial conditions (terrestrial leaves) to submerged conditions. However, the underlying mechanisms of underwater photosynthesis in those leaves are unknown. In this study, the biochemical and anatomical characteristics related to carbon acquisition for underwater photosynthesis were compared between terrestrial, submerged terrestrial (ST), and submerged leaves of H. difformis. The submerged leaves had a higher underwater photosynthetic rate under low dissolved inorganic carbon (DIC) conditions than the terrestrial leaves. The ST leaves showed rates between those of the terrestrial and submerged leaves. The ST and submerged leaves exhibited higher carbonic anhydrase activity than the terrestrial leaves. Only the submerged leaves had decreased epidermal cuticle and cell wall thicknesses. The ST and submerged leaves were observed to have thinner palisade cell wall thicknesses than the terrestrial leaves. Submergence acclimation in H. difformis is achieved by HCO3- utilization and anatomical change to decrease the resistance of CO2 diffusion from intercellular airspaces. The difference between the ST and submerged leaves results mainly from differing DIC flux into the leaves due to decreases in epidermal cuticle and cell wall thicknesses in the submerged leaves.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant sex-determining genes and the genetics of the evolution towards dioecy. 植物性别决定基因及其向雌雄异株进化的遗传学。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-06 DOI: 10.1093/jxb/eraf224

Gabriel A B Marais, Catarina Branco, Margarida Rocheta, Mathilde Dufay, Jeanne Tonnabel

{"title":"Plant sex-determining genes and the genetics of the evolution towards dioecy.","authors":"Gabriel A B Marais, Catarina Branco, Margarida Rocheta, Mathilde Dufay, Jeanne Tonnabel","doi":"10.1093/jxb/eraf224","DOIUrl":"https://doi.org/10.1093/jxb/eraf224","url":null,"abstract":"Thanks to significant advances in genomics and bioinformatics, research on plant sex-determining genes has made remarkable progress over the past decade. Since the discovery of the OGI-MeGI sex-determination system in persimmons in 2014, candidate sex-determining genes have been identified in a dozen flowering plant species. In this review, we examine these newly discovered genes and explore what they reveal about the genetic basis of dioecy evolution. While these genes are diverse, many belong to key developmental pathways previously described in other species. Two primary evolutionary routes from hermaphroditism to dioecy have been proposed: gynodioecy and monoecy. The gynodioecy pathway has been modeled with two genes and two sterility mutations, whereas recent theoretical work on the monoecy route suggests a gradual differentiation of sexual morphs involving multiple genes. Current data align with these models, particularly in the expected number of sex-determining genes. The gynodioecy pathway is typically associated with two or more genes, while the monoecy route varies, involving either one or two genes. From a molecular evolution perspective, loss-of-function mutations are frequent, but gene duplication appears to be the most common mechanism driving the emergence of new sex-determining genes. Future research should aim to characterize additional systems to gain a comprehensive view of plant sex determination and employ functional approaches to validate proposed candidate genes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in photosynthetic efficiency among maize cultivars and its implications for breeding strategy. 玉米品种间光合效率的变异及其对育种策略的影响。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-05 DOI: 10.1093/jxb/eraf249

Qiu-Yan Yang, Yu-Wen Zhang, Ning-Yu Liu, Hu Sun, Shi-Bao Zhang, Stefan Timm, Wei Huang

{"title":"Variation in photosynthetic efficiency among maize cultivars and its implications for breeding strategy.","authors":"Qiu-Yan Yang, Yu-Wen Zhang, Ning-Yu Liu, Hu Sun, Shi-Bao Zhang, Stefan Timm, Wei Huang","doi":"10.1093/jxb/eraf249","DOIUrl":"https://doi.org/10.1093/jxb/eraf249","url":null,"abstract":"Maize is one of the most important crops worldwide. Exploring the factors affecting photosynthetic efficiency alongside with the genetic variability within cultivars is fundamental to breed for high-yield maize. Despite its importance, there is limited knowledge about the variation in steady-state and dynamic photosynthetic efficiency among maize cultivars. Here, we investigated the anatomical and physiological mechanisms that contribute to the variability in photosynthetic CO2 assimilation under both, steady-state and fluctuating light conditions, respectively, in 12 high-yielding, farm-preferred maize cultivars. Under steady-state conditions, the saturating CO2 assimilation rate (Asat) varied from 50.2 to 63.1 µmol CO2 m-2 s-1. This variation was tightly related to the area ratio of mesophyll cells to bundle sheath cells, pointing out the role of leaf anatomy in determining Asat of maize. During the initial 5 minutes of light induction, we recorded a considerable range of cumulative CO2 fixation from 9.02 to 13.1 mmol m-2, with the loss of potential CO2 uptake (Closs) varying from 23% to 47% among the cultivars. This variation in Closs was primarily attributed to the kinetics of gs, which were significantly correlated to the expression of the ethylene synthesis genes ACS1 and GAD1, rather than stomatal density. Taking together, we discovered a greater degree of variation in dynamic photosynthetic efficiency compared to steady-state efficiency among the studied maize cultivars. This finding highlights the potential of manipulating gs kinetics as a valuable breeding target to enhance photosynthetic efficiency and yield in maize.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strigolactones Coordinate Barley Tillering and Grain Size. 独角甾内酯调节大麦分蘖和籽粒大小。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-06-04 DOI: 10.1093/jxb/eraf229

Jack H Kelly, Andrew J Gilmore, Apriadi Situmorang, Kenton D Porker, Marek Marzec, Matthew R Tucker, Philip B Brewer

引用次数: 0