Journal of Experimental Botany最新文献_第3页

Photoperiodic control of growth and reproduction in non-flowering plants. 光周期控制非开花植物的生长和繁殖。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-07 DOI: 10.1093/jxb/erae471

Durga Prasad Biswal, Kishore Chandra Sekhar Panigrahi

引用次数: 0

Ethylene antagonizes ABA and inhibits stomatal closure and chilling tolerance in rice.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-06 DOI: 10.1093/jxb/eraf052

Shuying Huang, Huanhuan Wang, Shiyan Liu, Shan Lu, Jian Hua, Baohong Zou

{"title":"Ethylene antagonizes ABA and inhibits stomatal closure and chilling tolerance in rice.","authors":"Shuying Huang, Huanhuan Wang, Shiyan Liu, Shan Lu, Jian Hua, Baohong Zou","doi":"10.1093/jxb/eraf052","DOIUrl":"https://doi.org/10.1093/jxb/eraf052","url":null,"abstract":"Chilling stress restricts the geographical distribution of rice and severely impacts its growth and development, ultimately reducing both yield and quality. The plant hormone ethylene is involved in plant stress responses; however, its role in rice chilling tolerance has not been thoroughly explored. This study reveals that ethylene negatively regulates chilling tolerance in rice by antagonizing the chilling tolerance-promoting effects of abscisic acid (ABA). Treatment with ethylene or its biosynthetic precursor, 1-aminocyclopropane-1-carboxylic acid (ACC), results in a reduced survival rate after chilling and delayed stomatal closure in response to chilling. There are two ethylene signaling-related Raf-like protein kinases, OsCTR1 and OsCTR2, which have overlapping functions in ethylene signaling; their loss-of-function mutants exhibit constitutive ethylene responses. The ctr1 ctr2 double mutant displays lower survival rates and slower stomatal closure under chilling stress compared to the wild type. In contrast, ABA treatment significantly enhances the survival rate of the wild type under chilling stress and promotes stomatal closure in response to chilling. Furthermore, ethylene inhibits the effects of ABA on chilling tolerance and stomatal closure. The ctr1 ctr2 double mutant fails to respond to external ABA treatment regarding stomatal closure and increased survival rate under chilling stress. In conclusion, our findings suggest that ethylene negatively regulates chilling tolerance in rice by inhibiting ABA-induced stomatal closure through the action of OsCTR1 and OsCTR2.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct domain regions of NIN and NLP1 mediate symbiotic and nitrate signaling in Medicago truncatula.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-05 DOI: 10.1093/jxb/eraf037

Jiang Wang, Mengdi Fu, Zhenpeng Luo, Jing Liu, Fang Xie

{"title":"Distinct domain regions of NIN and NLP1 mediate symbiotic and nitrate signaling in Medicago truncatula.","authors":"Jiang Wang, Mengdi Fu, Zhenpeng Luo, Jing Liu, Fang Xie","doi":"10.1093/jxb/eraf037","DOIUrl":"https://doi.org/10.1093/jxb/eraf037","url":null,"abstract":"Nodule Inception (NIN) and NIN-like protein 1 (NLP1), both belonging to the RWP-RK type transcription factors, play critical roles in plant development. Specifically, NIN is pivotal in facilitating root nodule symbiosis in nitrogen-starved conditions, while NLP1 coordinates nodulation in response to nitrate level. In this study, we conducted domain swapping experiments between NIN and NLP1 in Medicago truncatula to elucidate the functional significance of their respective domains. The findings reveal that the C-terminal regions, including the RWP-RK and PB1 domains of NIN, can substitute for those of NLP1, whereas reciprocal substitution do not yield equivalent outcomes. Moreover, our data emphasize the critical role of PB1-mediated interactions for NLP1's activity, a feature not essential for NIN. Additionally, the N-terminal segment, conserved in NLPs but containing deletions or mutations in NIN, is essential for the proper functioning of both NIN and NLP1. Collectively, our research suggests the evolutionary divergence of NIN from ancestral NLPs, indicating specific adaptations that have enabled NIN as a central regulator in root nodulation processes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of m6A Modification and Transcript Quantity on mRNA Composition in Plant Stress Granules.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-05 DOI: 10.1093/jxb/eraf046

Dawid Jakub Kubiak, Michal Wojciech Szczesniak, Karolina Ostrowska, Dawid Bielewicz, Susheel Sagar Bhat, Katarzyna Niedojadlo, Zofia Szweykowska-Kulinska, Artur Jarmolowski, Rupert George Fray, Janusz Niedojadlo

{"title":"Impact of m6A Modification and Transcript Quantity on mRNA Composition in Plant Stress Granules.","authors":"Dawid Jakub Kubiak, Michal Wojciech Szczesniak, Karolina Ostrowska, Dawid Bielewicz, Susheel Sagar Bhat, Katarzyna Niedojadlo, Zofia Szweykowska-Kulinska, Artur Jarmolowski, Rupert George Fray, Janusz Niedojadlo","doi":"10.1093/jxb/eraf046","DOIUrl":"https://doi.org/10.1093/jxb/eraf046","url":null,"abstract":"Stress granules (SGs) are cytoplasmic structures that emerge in response to unfavorable environmental conditions. The mechanisms governing the accumulation of transcripts in SGs are only partially understood. Despite the recognized role of N6-methyladenosine (m6A) in plant transcriptome regulation, its impact on SGs' composition and assembly remains elusive. In Lupinus angustifolius, SGs display a distinctive bi-zonal structure comprising of a ring and a central area with differences in ultrastructure and composition. Subsequent to the transcriptome analysis, specific mRNA were chosen to investigate their localization within SGs and assess m6A levels. Transcripts of hypoxia-responsive genes (ADH1 and HUP7) showed significantly lower levels of m6A compared to housekeeping genes, but only ADH1 was absent in SGs. HUP7 mRNA, characterized by a low quantity of m6A, is present both in the SGs and cytoplasm, probably due to extremely high expression level. The m6A was observed only during the assembly of SGs. In mutants of Arabidopsis thaliana with reduced levels of m6A, ECT2 (reader of m6A) was not observed in SGs, and poly(A) RNA levels and the number of SGs were reduced. In summary, our findings demonstrate a limited impact of m6A modification on SGs assembly. However, the interplay between m6A modification and the overall transcript quantity in the cytoplasm appears to play a regulatory role in mRNA partitioning and assembly of SGs.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grapevine leafroll-associated virus 3: A global threat to the grape and wine industries but a gold mine for scientific discovery. 葡萄卷叶相关病毒 3：葡萄和葡萄酒业的全球威胁，但却是科学发现的金矿。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-04 DOI: 10.1093/jxb/eraf039

Catherine Fust, Patrick Lameront, Mehdi Shabanian, Yashu Song, Raied Abou Kubaa, Rachelle Bester, Hans J Maree, Maher Al Rwahnih, Baozhong Meng

{"title":"Grapevine leafroll-associated virus 3: A global threat to the grape and wine industries but a gold mine for scientific discovery.","authors":"Catherine Fust, Patrick Lameront, Mehdi Shabanian, Yashu Song, Raied Abou Kubaa, Rachelle Bester, Hans J Maree, Maher Al Rwahnih, Baozhong Meng","doi":"10.1093/jxb/eraf039","DOIUrl":"https://doi.org/10.1093/jxb/eraf039","url":null,"abstract":"Grapevine leafroll disease is the most damaging viral disease afflicting global grape and wine production. Of the five viruses likely associated with the disease, grapevine leafroll-associated virus 3 (GLRaV3) is believed to be the predominant agent, albeit its role as the causal agent has remained uncertain. GLRaV3 (species Ampelovirus trivitis, genus Ampelovirus, family Closteroviridae) has the third largest single-stranded, positive-sense RNA genome among plant viruses at ~18.5 kb, only surpassed by two other members of the family Closteroviridae, citrus tristeza virus, and GLRaV1. GLRaV3 is unique among plant viruses in several ways including the size of its genome, the long non-coding regions, and its association with the outer mitochondrial membrane for viral replication. Unfortunately, our understanding of the molecular mechanisms governing GLRaV3 genome replication, gene expression and virus-host interactions is poor due to many factors, including the unavailability of infectious cDNA clones and effective experimental system to initiate grapevine infection with viral clones until recently. In this review, we capture some recent advances in GLRaV3 research towards the establishment of infectious clones, grapevine inoculation systems, as well as approaches towards elucidating the function of GLRaV3-encoded proteins. We also present a working model to explain GLRaV3 pathogenesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Severe water stress impact on drought resistance mechanisms and hydraulic vulnerability segmentation in grapevines: the role of rootstock. 严重的水分胁迫对葡萄树抗旱机制和水力脆弱性分段的影响：砧木的作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-04 DOI: 10.1093/jxb/eraf044

L Flor, G Toro, M Carriquí, I Buesa, A Sabater, H Medrano, J M Escalona

{"title":"Severe water stress impact on drought resistance mechanisms and hydraulic vulnerability segmentation in grapevines: the role of rootstock.","authors":"L Flor, G Toro, M Carriquí, I Buesa, A Sabater, H Medrano, J M Escalona","doi":"10.1093/jxb/eraf044","DOIUrl":"https://doi.org/10.1093/jxb/eraf044","url":null,"abstract":"Severe water stress can lead to hydraulic disfunction, reducing plant conductance or even causing death. Some plants exhibit hydraulic vulnerability segmentation between organs to reduce this risk. However, its role in influencing drought tolerance and resistance in grafted plants, such as grapevine, remains unclear. This study aimed to evaluate the physiological responses, drought tolerance, hydraulic vulnerability segmentation and xylem anatomy of two-year-old Vitis vinifera cv. Tempranillo scion grafted onto two rootstocks: 110-Richter (110R) and Sélection Oppenheim 4 (SO4). After subjecting the plants to drought conditions until the onset of embolism in the leaf (P12), we analysed the physiological consequences during recovery. Grapevine exhibits hydraulic vulnerability segmentation not only within scion organs but also between the scion and rootstock. Although no differences in scion drought tolerance and embolism resistance were observed between combinations, Tempranillo-110R exhibited higher leaf minimum conductance, leaf P12 values and root biomass. In contrast, Tempranillo-SO4 displayed larger vessel diameter and higher hydraulic conductance. These differences may explain the slower recovery of Tempranillo-110R compared to Tempranillo-SO4, which showed higher stomatal and root-to-stem hydraulic conductance. These findings suggest that rootstock selection should consider drought resilience alongside vigour and productivity, especially given the increasing the concurrence of severe drought periods due to climate change.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The single-berry metabolomic clock paradigm reveals new stages and metabolic switches during grapevine berry development.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-02-02 DOI: 10.1093/jxb/eraf038

Flora Tavernier, Stefania Savoi, Laurent Torregrosa, Philippe Hugueney, Raymonde Baltenweck, Vincent Segura, Charles Romieu

{"title":"The single-berry metabolomic clock paradigm reveals new stages and metabolic switches during grapevine berry development.","authors":"Flora Tavernier, Stefania Savoi, Laurent Torregrosa, Philippe Hugueney, Raymonde Baltenweck, Vincent Segura, Charles Romieu","doi":"10.1093/jxb/eraf038","DOIUrl":"https://doi.org/10.1093/jxb/eraf038","url":null,"abstract":"Asynchronicity causes metabolic chimerism in usual grapevine phenological stages, calling for a revisit of berry development at the individual fruit scale. To reveal the dynamics of metabolite composition in grapevine berries without phenological a priori, a dataset of 9,256 ions was obtained on 125 fruits at different stages by non-targeted ultra-performance liquid chromatography coupled to high-resolution mass spectrometry. This large metabolomic dataset was submitted to an analysis workflow combining classification and dimension reduction tools. This led to the clustering of metabolites into 12 specific kinetic patterns and a metabolome-based definition of the pericarp intrinsic clock outperforming expert timing procedures. Such increased temporal resolution enabled the identification of metabolite clusters that are annunciative of the onset of ripening, noticeably characterized by transient lipidic changes and the start of ABA accumulation. We also highlighted a cluster of stilbenes that accumulate during terminal fruit shriveling, after the arrest of phloem unloading. This non-targeted approach enables a more precise characterization of grapevine berry development through the concept of metabolomic clock. The discovery of new metabolic milestones of berry development paves the way toward a better assessment of the impact of environmental changes on the metabolism of non-climacteric fruit, in different genotypes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plastidial glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-30 DOI: 10.1093/jxb/eraf041

Aakanksha Singh, Rajadurga Rajendran, Mark Aurel Schöttler, Xiaoping Li, Qingting Liu, Sidratul Nur Muntaha, Joerg Fettke

{"title":"The plastidial glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana.","authors":"Aakanksha Singh, Rajadurga Rajendran, Mark Aurel Schöttler, Xiaoping Li, Qingting Liu, Sidratul Nur Muntaha, Joerg Fettke","doi":"10.1093/jxb/eraf041","DOIUrl":"https://doi.org/10.1093/jxb/eraf041","url":null,"abstract":"The plastidial α-glucan phosphorylase (PHS1) can catalyze the elongation and degradation of glucans, but its exact physiological role in plants is not completely deciphered. A plethora of studies have indicated that PHS1 is involved in transitory starch turnover, both in photosynthetic tissues as well as reserve starch accumulation in sink organs of multiple species, by exerting its effects on the plastidial maltodextrin pools. Recent studies have also established its role in the mobilization of short maltooligosaccharides (MOSs), thereby assisting in starch granule initiation. This paper highlights our current findings from studying four constitutive double-knock out mutants related to the plastidial maltodextrin metabolism namely phs1dpe1, phs1ptst2, phs1pgm1 and phs1isa3 of Arabidopsis thaliana. Our studies have indicated different effects on carbon partitioning in these double mutants. The carbon allocation between starch and sucrose in different double mutants varied with respect to time and light conditions with significant overall changes in phs1dpe1. Furthermore, a potential time specific function of PHS1 in maltodextrin metabolism has come to light. Changes in maltodextrin turnover exerted effects on the starch granule number and size in the double mutants especially phs1dpe1. The characterized double mutants have been further assessed in terms of photosynthetic efficiency, and starch parameters such as internal structure and morphology in detail. Our results have shown that the different photosynthetic parameters in pgm1 and its corresponding double mutant were affected relative to WT and phs1. However, other double mutants were not impaired in terms of photosynthetic efficiency despite alterations in their MOS levels.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phototropin 2 mediates daily cold priming to promote light responses in Arabidopsis.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-30 DOI: 10.1093/jxb/eraf040

Minoru Noguchi, Issei Keino, Hitomi Takahashi, Shota Yamauchi, Mami Fujisawa, Ken Haga, Tatsuya Sakai, Atsushi Takemiya, Yutaka Kodama

{"title":"Phototropin 2 mediates daily cold priming to promote light responses in Arabidopsis.","authors":"Minoru Noguchi, Issei Keino, Hitomi Takahashi, Shota Yamauchi, Mami Fujisawa, Ken Haga, Tatsuya Sakai, Atsushi Takemiya, Yutaka Kodama","doi":"10.1093/jxb/eraf040","DOIUrl":"https://doi.org/10.1093/jxb/eraf040","url":null,"abstract":"Organisms adapt to predictable environmental changes via a biological mechanism called priming. Phototropin (phot) is a plant-specific blue light photoreceptor that mediates daily light-induced responses, such as chloroplast relocation, stomatal opening, and phototropism, to optimize photosynthesis. Phot also functions as a thermosensor for chloroplast relocation that may sense daily temperature decreases at night, thereby modulating light-induced responses at dawn; however, this hypothesis has not yet been fully explored. Here, we reveal that phot mediates daily cold priming to promote stomatal opening and phototropism in Arabidopsis (Arabidopsis thaliana) under dawn-mimicking conditions. A cold pretreatment in the dark enhanced subsequent blue light-induced stomatal opening and phototropism at normal temperatures, suggesting that daily cold priming is involved in these physiological responses. Arabidopsis has two phot proteins (phot1 and phot2), and we show that phot2 clearly mediates the cold priming of stomatal opening and phototropism. Cold priming appears to be based on phot-mediated thermosensing just before dawn, which plants use to optimize their light-induced responses in anticipation of dawn.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating how reproductive traits in rice respond to abiotic stress.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-29 DOI: 10.1093/jxb/eraf031

Claudia Maria Liberatore, Marco Biancucci, Juan Ignacio Ezquer, Veronica Gregis, Maurizio Di Marzo

引用次数: 0