Journal of Experimental Botany最新文献_第4页

Lighting the Path: How Light Signaling Regulates Stomatal Movement and Plant Immunity.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-14 DOI: 10.1093/jxb/erae475

Nidhi Singh, Mrunmay Kumar Giri, Debasis Chattopadhyay

{"title":"Lighting the Path: How Light Signaling Regulates Stomatal Movement and Plant Immunity.","authors":"Nidhi Singh, Mrunmay Kumar Giri, Debasis Chattopadhyay","doi":"10.1093/jxb/erae475","DOIUrl":"https://doi.org/10.1093/jxb/erae475","url":null,"abstract":"Stomata, the small pores on the surfaces of leaves and stems, are crucial for gas exchange in plants and also play a role in defense against pathogens. The stomatal movement is not only influenced by surrounding light conditions but also by the presence of foliar pathogens. To put it more crisply, certain light wavelengths such as blue or strong red light, cause stomatal opening, which tragically makes it easier for bacteria to enter through opened stomata and causes disease progression in plants. Illumination of blue or intense red light autophosphorylates phototropin, a blue light photoreceptor protein kinases that in turn activates signaling cascade to open stomata. Undoubtedly stomatal defense is a fascinating aspect of plant immunology, especially in plant-foliar pathogen interaction. During these interactions, stomata fundamentally serve as entry points for intrusive pathogens and initiate plant defense signaling cascade. The present review highlights how the light-activated photoreceptors like cryptochromes (CRYs), phytochromes (phys), and UV-receptors (UVRs) influence the stomatal movement and defense signaling after foliar pathogen intrusion. It also explores the link between stomatal defense, light signaling, and plant immunity, which is vital for safeguarding crops against pathogens.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unravelling the impact of domestication on competitive ability in Durum Wheat: A Phenotypic Plasticity Perspective. 揭示驯化对杜伦麦竞争能力的影响：表型可塑性视角。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-14 DOI: 10.1093/jxb/erae480

Taïna Lemoine, Cyrille Violle, Eric Antoine Gonzalez, Mathis Gaubert, Aline Rocher, Hélène Fréville, Florian Fort

{"title":"Unravelling the impact of domestication on competitive ability in Durum Wheat: A Phenotypic Plasticity Perspective.","authors":"Taïna Lemoine, Cyrille Violle, Eric Antoine Gonzalez, Mathis Gaubert, Aline Rocher, Hélène Fréville, Florian Fort","doi":"10.1093/jxb/erae480","DOIUrl":"https://doi.org/10.1093/jxb/erae480","url":null,"abstract":"The need to address the impact of domestication on plant traits is frequently highlighted in modern agriculture. It is often argued that domesticated plants have lost competitive ability due to reduced phenotypic plasticity. This study examines whether domestication has affected competitive ability, functional trait values, and plasticity in durum wheat across 39 genotypes representing four key stages of domestication, from wild progenitors to modern elite varieties. Plants were grown in pots, both alone and in competition with a same neighboring genotype. Biomass, and above- and belowground traits were measured at the end of the vegetative stage. Our results show that the three domesticated groups lost less biomass in response to competition compared to their wild progenitors. All genotypes developed thinner leaves and thicker roots when grown with a neighbor. While wild progenitors exhibited the highest plasticity, this did not translate to a greater competitive ability. These findings challenge the theoretical expectation that domesticated plants are less suited for competition. Instead, they suggest that domesticated plants perform well in competitive environments and question the need to reintroduce wild traits to improve competitive ability.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative detection of the maize phytocytokine Zip1 utilizing ELISA. 利用酶联免疫吸附法定量检测玉米植物细胞因子 Zip1。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-14 DOI: 10.1093/jxb/erae423

Maurice Koenig, Zarah Sorger, Shania Pin Yin Keh, Gunther Doehlemann, Johana C Misas Villamil

{"title":"Quantitative detection of the maize phytocytokine Zip1 utilizing ELISA.","authors":"Maurice Koenig, Zarah Sorger, Shania Pin Yin Keh, Gunther Doehlemann, Johana C Misas Villamil","doi":"10.1093/jxb/erae423","DOIUrl":"https://doi.org/10.1093/jxb/erae423","url":null,"abstract":"Plant signaling peptides, also known as phytocytokines, play a crucial role in cell-to-cell communication during plant development and immunity. The detection of small peptides in plant tissues is challenging and often relies on time-consuming and cost-intensive approaches. Here, we present an ELISA-based assay as a rapid and cost-effective method for the detection of naturally released peptides in plant tissues. Our ELISA-based method was developed to detect Zip1, a 17-amino-acid phytocytokine derived from Zea mays that elicits salicylic acid signaling in maize leaves. Using a custom peptide-antibody, we designed an experimental pipeline to achieve peptide specificity, selectivity, and sensitivity allowing the detection of the Zip1 peptide in complex biological samples. As a proof of concept, we first overexpressed the precursor molecule PROZIP1 in Nicotiana benthamiana and in transfected maize protoplasts and monitored the release of Zip1-containing peptides. In a second approach we treated maize leaves with salicylic acid to induce native PROZIP1 expression and processing. Using ELISA, we were able to quantify native Zip1 signals with a detection limit in the nanogram range, which allowed us to detect different Zip1-containing peptides in plant material. This method can be adapted for the detection and quantification of a variety of plant signaling peptides.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Energy conservation or expense? A possible dilemma under combined stresses of salinity and submergence in rice.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-13 DOI: 10.1093/jxb/erae499

Koushik Chakraborty, Subhankar Mondal, Swagatika Tripathy, Priyanka Jena, Lotan Kumar Bose, Krishnendu Chattopadhyay

{"title":"Energy conservation or expense? A possible dilemma under combined stresses of salinity and submergence in rice.","authors":"Koushik Chakraborty, Subhankar Mondal, Swagatika Tripathy, Priyanka Jena, Lotan Kumar Bose, Krishnendu Chattopadhyay","doi":"10.1093/jxb/erae499","DOIUrl":"https://doi.org/10.1093/jxb/erae499","url":null,"abstract":"Salinity and flooding are two major production impediments affecting rice cultivation in coastal agro-ecosystems. We investigated how rice plants use two contrasting strategies such as energy conservation (for submergence tolerance) and energy expenditure (for ion exclusion) to adapt to the combined stresses of saline water submergence (SWS). Pot and hydroponic experiments were conducted using four selected rice genotypes carrying Sub1 (Submergence1) and/or Saltol (Salinity tolerance) QTLs in their genetic background and exposed them to salinity and submergence stresses individually and combined under controlled experimental conditions. We found that Sub1-containing submergence-tolerant lines performed better under SWS, where Saltol-containing Na+-excluder could not able to survive. The presence of thicker leaf gas film (LGF) and higher epicuticular wax helped longer underwater retention of LGF in Sub1-lines supporting survival under SWS. Thicker LGF significantly delayed Na+ entry to the leaves under anoxic conditions. Genotypes having good Na+-exclusion potential, but thinner LGF ultimately accumulated more Na+ in the leaf tissue under SWS. The lowest leaf carbohydrate depletion was observed in FR13A, whereas non-Sub1 associated escape coupled with ion exclusion utilized the highest carbohydrate reserve in FL478 under SWS treatment. Overall, this study uncovers that the Sub1-mediated quiescence strategy complemented by higher tissue tolerance ability is a more suitable mechanistic adaptation than ion exclusion under saline water submergence in rice.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid on-site detection of crop RNA viruses using CRISPR/Cas13a.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-10 DOI: 10.1093/jxb/erae495

Hagit Hak, Steffen Ostendorp, Anton Reza, Shany Ishgur Greenberg, Gur Pines, Julia Kehr, Ziv Spiegelman

{"title":"Rapid on-site detection of crop RNA viruses using CRISPR/Cas13a.","authors":"Hagit Hak, Steffen Ostendorp, Anton Reza, Shany Ishgur Greenberg, Gur Pines, Julia Kehr, Ziv Spiegelman","doi":"10.1093/jxb/erae495","DOIUrl":"https://doi.org/10.1093/jxb/erae495","url":null,"abstract":"Plant viruses are destructive pathogens for various crop species. Rapid, sensitive, and specific detection is crucial for the effective containment of emerging and resistance-breaking viruses. CRISPR/Cas has been established as a new tool for plant virus identification. However, its application for direct detection of viruses in the field is still limited. In this study, we present a CRISPR/Cas13a-based method for rapid detection of different viruses directly from RNA of several crop species, including tomato, cucumber and rapeseed. This method was used to identify the emerging tomato brown rugose fruit virus (ToBRFV), a prominent pathogen in tomato cultivation, and distinguish it from closely related viruses in infected tomato plants. ToBRFV could be identified in a 100-fold dilution and early during infection, prior to the onset of viral symptoms. Finally, we developed a user-friendly, extraction-free, 15-minute protocol for on-site virus detection using a portable fluorescent viewer and a mobile phone camera. This protocol was successfully applied for ToBRFV identification in several commercial greenhouses. These results demonstrate that CRISPR/Cas13a is a robust technology for on-site detection of multiple viruses in different crop plants. This method could be swiftly adapted to identify newly emerging pests, which threaten global food security.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RD21-like proteases: key effector hubs in plant-pathogen interactions.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-10 DOI: 10.1093/jxb/erae496

Jie Huang, Renier A L van der Hoorn

引用次数: 0

Haplotyped genome mapping and functional characterisation of a blueberry anthocyanin acetyltransferase (AAT) controlling the accumulation of acylated anthocyanins.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-07 DOI: 10.1093/jxb/erae489

Andrew P Dare, Chen Wu, Jose I Carvajal, Han M Nguyen, Catrin S Günther, Cyril Hamiaux, Sarah Bailey, Cecilia Deng, Molla F Mengist, Massimo Iorizzo, Toshi M Foster, David Chagné, Sara Montanari, Richard V Espley

{"title":"Haplotyped genome mapping and functional characterisation of a blueberry anthocyanin acetyltransferase (AAT) controlling the accumulation of acylated anthocyanins.","authors":"Andrew P Dare, Chen Wu, Jose I Carvajal, Han M Nguyen, Catrin S Günther, Cyril Hamiaux, Sarah Bailey, Cecilia Deng, Molla F Mengist, Massimo Iorizzo, Toshi M Foster, David Chagné, Sara Montanari, Richard V Espley","doi":"10.1093/jxb/erae489","DOIUrl":"https://doi.org/10.1093/jxb/erae489","url":null,"abstract":"Blueberry has a diversity of anthocyanins that confer its characteristic, blue-coloured skin. Whilst most cultivars produce only anthocyanin glycosides, some can add aliphatic or aromatic groups to the sugar moiety to create acylated anthocyanins. Due to their enhanced stability, acylated anthocyanins represent an attractive breeding target in blueberry. In this study a haplotype-resolved assembly of a previously identified quantitative trait locus on chromosome 2 of 'Hortblue Petite' (Vaccinium corymbosum) was created to identify candidate anthocyanin acyltransferase genes. One full-length gene (VcAAT1a) was selected, based on qPCR expression profiling and transient expression in tobacco leaves and strawberry and blueberry fruit flesh. In all three systems VcAAT1a was able to produce a range of acylated anthocyanins in plantae. Recombinant VcAAT1a protein demonstrated that, while VcAAT1a was able to act on both anthocyanin 3-O-glucosides and 3-O-galactosides, it could only utilise acetyl CoA as an acyl donor. Protein modelling using AlphaFold suggested that this restricted range in acyl donors may be due to a spatially restricted sub-pocket in the acyl binding site of VvAAT1. Finally, LUC/REN promoter activation assays revealed that the VcAAT1a promoter was transactivated by the VcMYBPA1 and VcMYBPA2 transcription factors, further expanding our knowledge of anthocyanin regulation in blueberry.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photoperiod-H1 effects on barley floret development and fertility under contrasting photoperiods and PHYC backgrounds.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-06 DOI: 10.1093/jxb/erae492

Jorge D Parrado, Roxana Savin, Gustavo A Slafer

{"title":"Photoperiod-H1 effects on barley floret development and fertility under contrasting photoperiods and PHYC backgrounds.","authors":"Jorge D Parrado, Roxana Savin, Gustavo A Slafer","doi":"10.1093/jxb/erae492","DOIUrl":"https://doi.org/10.1093/jxb/erae492","url":null,"abstract":"The photoperiod insensitive allele of Photoperiod-H1 (ppd-H1) increases spike fertility in barley, both indirectly by lengthening flowering time and directly when flowering time is accelerated under extra-long photoperiods. To determine if the effect of PPD-H1 on spike fertility is related to the initiation or the mortality of spikelets/florets, we performed detailed analysis of the dynamics of floret development along the barley spikes. Four near-isogenic lines (NILs) combining ppd-H1 and Ppd-H1 alleles with two PHYTOCHROME C (PhyC-l and PhyC-e) backgrounds were compared under 12- and 24-hour photoperiods. The ppd-H1 line consistently showed higher survival rates of floret primordia. Fertility differences were observed within spikes, with the insensitive ppd-H1 line improving fertility in the less-favoured distal florets, and even inducing more developmental progress before floret mortality in apical spikelets. The ppd-H1 allele constitutively enhanced floret primordia survival, as the effect was seen even under long photoperiods when lines had similar phenology. The dynamics of floret development affecting spike fertility resulted in a parallel effect on grain setting. Together, these results indicate that photoperiod insensitivity in barley contributes to greater yield potential by reducing floret primordia mortality and improving spike fertility.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the bamboo SWEET gene family reveals that Dendrocalamus farinosus SWEET14 is involved in source-sink carbohydrate partitioning. 对竹子 SWEET 基因家族的分析表明，Dendrocalamus farinosus SWEET14 参与碳水化合物的源-汇分配。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-05 DOI: 10.1093/jxb/erae493

Xiaoyan Gu, Jiahui Peng, Qian Ou, Sen Chen, Wei Feng, Yiqin Huang, Bin Deng, Ying Cao, Shanglian Hu

{"title":"Analysis of the bamboo SWEET gene family reveals that Dendrocalamus farinosus SWEET14 is involved in source-sink carbohydrate partitioning.","authors":"Xiaoyan Gu, Jiahui Peng, Qian Ou, Sen Chen, Wei Feng, Yiqin Huang, Bin Deng, Ying Cao, Shanglian Hu","doi":"10.1093/jxb/erae493","DOIUrl":"https://doi.org/10.1093/jxb/erae493","url":null,"abstract":"In higher plants, SWEET genes play a crucial role in source-sink carbohydrate partitioning. Dendrocalamus farinosus is an economic bamboo species because of its high fiber content and rapid growth. The transportation of photosynthetic products is essential for bamboo growth. Here, we identified 50 major SWEET genes in D. farinosus. Phylogenetic analysis indicated that DfSWEET14 and DfSWEET44 were homologs of OsSWEET11 and OsSWEET4, respectively. DfSWEET5, DfSWEET14 and DfSWEET44 were highly expressed in leaf veins, stems and roots, respectively, and their expression was responsive to glucose and sucrose. DfSWEET9 and DfSWEET28 were involved in photosynthetic product distribution, ABA/MeJA signaling and pathogen responses. DfSWEET14 was localized to the plasma membrane, and was highly expressed in lateral buds and young shoots only. The overexpression of DfSWEET14 in wheat resulted in higher root/shoot biomass ratio under drought stress and increases in grain-filling duration, seed yield, net photosynthetic capacity, soluble sugar content, seed size and grain weight. This suggests that DfSWEET14 is involved in sucrose partitioning from leaves to grains. These results provide new insights into genetic improvement of bamboo and into the breeding of other economic crops for high yield by manipulation of source-sink carbohydrate partitioning and drought responses.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual nematode infection in Brassica nigra affects shoot metabolome and aphid survival in distinct contrast to single-species infection. 甘蓝中线虫的双重感染对嫩枝代谢组和蚜虫存活的影响与单一物种感染形成鲜明对比。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae364

Jessil Ann Pajar, Pius Otto, April Lyn Leonar, Stefanie Döll, Nicole M van Dam

{"title":"Dual nematode infection in Brassica nigra affects shoot metabolome and aphid survival in distinct contrast to single-species infection.","authors":"Jessil Ann Pajar, Pius Otto, April Lyn Leonar, Stefanie Döll, Nicole M van Dam","doi":"10.1093/jxb/erae364","DOIUrl":"10.1093/jxb/erae364","url":null,"abstract":"Previous studies showed that aphid performance was compromised on Brassica nigra infected by root-lesion nematodes (Pratylenchus penetrans, Pp), but less, or positively influenced by root-knot nematode (Meloidogyne spp.) infection. These experiments were on single-species nematode infections, but roots can be infected naturally with several nematode species simultaneously. We performed greenhouse assays to assess the effects of single [Meloidogyne incognita (Mi) or Pp] and concurrent (MP) nematode infections on aphid performance. Using targeted and untargeted profiling of leaf and phloem metabolomes, we examined how single and concurrent nematode infections affect shoot metabolomes, and elucidated the possible consequences for aphid performance. We found that the metabolic response to double-infection is different from that to single-species infections. Moreover, Mi and Pp infections triggered discrete changes in B. nigra leaf and phloem metabolic profiles. Both Pp and MP infections reduced aphid survival, suggesting that the biological effect could primarily be dominated by Pp-induced changes. This concurred with increased indole glucosinolates and hydroxycinnamic acid levels in the leaves, in particular the putative involvement of salicylic acid-2-O-β-d-glucoside. This study provides evidence that concurrent infection by different nematode species, as is common in natural environments, is associated with distinct changes in aboveground plant metabolomes, which are linked to differences in the survival of an aboveground herbivore.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7317-7336"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0