Journal of Experimental Botany最新文献_第4页

Correction to: Two transcriptional activators of N-acetylserotonin O-methyltransferase 2 and melatonin biosynthesis in cassava.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-29 DOI: 10.1093/jxb/eraf002

引用次数: 0

Molecular mechanisms affected by boron deficiency in root and shoot meristems of plants. 植物根部和芽分生组织受缺硼影响的分子机制

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-28 DOI: 10.1093/jxb/eraf036

Liuyang Chu, Cay Christin Schäfer, Michaela S Matthes

{"title":"Molecular mechanisms affected by boron deficiency in root and shoot meristems of plants.","authors":"Liuyang Chu, Cay Christin Schäfer, Michaela S Matthes","doi":"10.1093/jxb/eraf036","DOIUrl":"https://doi.org/10.1093/jxb/eraf036","url":null,"abstract":"Boron deficiency is an abiotic stress that negatively impacts plant growth and yield worldwide. Boron deficiency primarily affects the development of plant meristems, groups of stem cells critical for all postembryonic tissue growth. The link between boron and meristem development was first established in 1923, when boron's essentiality was discovered. It remains, however, unclear whether boron directly integrates into molecular meristem pathways. In addition to its stabilizing function in the primary cell wall, growing evidence suggests roles of boron in various molecular processes including phytohormone cascades. These indications help enhance a mechanistic understanding of why boron is crucial for proper meristem development. In this review we compile and discuss molecular pathways influenced by boron availability in Arabidopsis (Arabidopsis thaliana), maize (Zea mays), rice (Oryza sativa), and oilseed rape (Brassica napus) with a focus on the auxin-, ethylene-, and cytokinin-mediated hormone cascades. We particularly compare and contrast phenotypic and molecular adaptations of shoot and root meristems to boron deficiency and pinpoint tissue-specific differences.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-28 DOI: 10.1093/jxb/eraf035

Sandrine Kappel, Maureen J Frieboes, Ryo Yokoyama, Christian Kappel, Szilvia Z Tóth, Alisdair R Fernie, Peter Jahns, Nicholas Smirnoff, Fayezeh Aarabi, Ute Armbruster

{"title":"Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.","authors":"Sandrine Kappel, Maureen J Frieboes, Ryo Yokoyama, Christian Kappel, Szilvia Z Tóth, Alisdair R Fernie, Peter Jahns, Nicholas Smirnoff, Fayezeh Aarabi, Ute Armbruster","doi":"10.1093/jxb/eraf035","DOIUrl":"https://doi.org/10.1093/jxb/eraf035","url":null,"abstract":"In nature, environmental conditions strongly fluctuate, frequently subjecting plants to periods of immediate photo-oxidative stress. The small molecule ascorbate allows plants to cope with such stress conditions. Ascorbate scavenges reactive oxygen species and enables the rapid and full induction of photoprotective non-photochemical quenching (NPQ). NPQ is dependent on zeaxanthin (Zx), which requires ascorbate as the electron donor during its synthesis by the violaxanthin de-epoxidase. The VTC2 gene encodes for one of two isoforms of GDP-L-galactose phosphorylase, the rate-controlling enzyme of ascorbate biosynthesis. In the current study, by including a newly identified vtc2 allele, we found that loss of VTC2 depleted ascorbate mainly from the mature leaves and thereby limited NPQ specifically in this tissue. Growth in fluctuating light and controlled climate suppressed the slow NPQ induction phenotype of vtc2 mature leaves to some degree. This was concurrent with a constitutively higher accumulation of zeaxanthin under this condition. When plants were shifted to natural conditions, with strongly fluctuating light and temperature, the ascorbate deficient mature leaves of vtc2 bleached. Together, our results reveal developmental and environmental effects on VTC2-dependent ascorbate accumulation and function.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning of sulfur flows and sulfur seed metabolism in oilseed rape facing sulfate limited conditions.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf028

Philippe D'Hooghe, Stanislav Kopriva, Jean-Christophe Avice, Jacques Trouverie

{"title":"Tuning of sulfur flows and sulfur seed metabolism in oilseed rape facing sulfate limited conditions.","authors":"Philippe D'Hooghe, Stanislav Kopriva, Jean-Christophe Avice, Jacques Trouverie","doi":"10.1093/jxb/eraf028","DOIUrl":"https://doi.org/10.1093/jxb/eraf028","url":null,"abstract":"The response of oilseed rape to sulfur (S) restriction usually consists of increasing the components of S utilization efficiency (absorption, assimilation and remobilization) to provide S to seeds. However, source-sink relationships and S management in developing seeds under sulfate restriction are poorly understood. To address this, impacts of sulfate restrictions applied at \"visible bud\" or \"start of pod filling\" stages were studied with two genotypes (Aviso, Capitol) showing similar seed yield but higher seed weight and lower number of seeds per plant for Capitol under non-limited conditions. S flows at the whole plant level (using 34S-sulfate labelling) and S metabolism changes (S-compounds, ATP sulfurylase and adenosine 5'phosphosulfate reductase (APR) activities) were followed during seed development. Seed yield, protein quality and accumulation of S metabolites were affected by sulfate restriction less and later in Aviso than in Capitol. This is related to higher S uptake and stronger remobilization of S from vegetative organs to seeds during early seed development in response to sulfate restriction. A higher seed APR activity was observed for Capitol in response to sulfate limitation, suggesting that APR is not limiting for sulfate assimilation and that seed S metabolism is principally devoted to S-amino acids and protein synthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conserved and novel roles of the bHLH transcription factor SPATULA in tomato.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf029

Eduardo Martínez-Estrada, Judith Jazmín Bernal-Gallardo, Pablo López-Gómez, Daniela de la Mora-Franco, Marisela Celso-Espinoza, Moisés Guerrero-Esperanza, David Díaz-Ramírez, Nayelli Marsch-Martínez, José Juan Ordaz-Ortiz, Stefan de Folter

{"title":"Conserved and novel roles of the bHLH transcription factor SPATULA in tomato.","authors":"Eduardo Martínez-Estrada, Judith Jazmín Bernal-Gallardo, Pablo López-Gómez, Daniela de la Mora-Franco, Marisela Celso-Espinoza, Moisés Guerrero-Esperanza, David Díaz-Ramírez, Nayelli Marsch-Martínez, José Juan Ordaz-Ortiz, Stefan de Folter","doi":"10.1093/jxb/eraf029","DOIUrl":"https://doi.org/10.1093/jxb/eraf029","url":null,"abstract":"Arabidopsis has served as a model plant for studying the genetic networks that guide gynoecium development. However, less is known about other species such as tomato, a model for fleshy fruit development and ripening. Here, we study in tomato the transcription factor SPATULA (SPT), a bHLH-family member that in Arabidopsis is known to be important for gynoecium development. We analysed the expression of SlSPT during flower and fruit development, and its interaction with proteins previously reported as interactors of AtSPT in the gynoecium. We also generated and characterised loss-of-function tomato lines using CRISPR-Cas9. The results show that SlSPT forms homodimers and partially conserves the interactions reported in Arabidopsis with some HECATE proteins, and has a role in floral organ development, particularly in stamen fusion, style and stigma development, and trichome formation on the carpels. Furthermore, lack of SlSPT causes altered exocarp pigmentation. A metabolomic analysis of the exocarp showed perturbations in several pathways in the slspt mutant, with the flavonoid biosynthesis being the most affected, which could potentially impact the nutritional value of the fruit. In summary, the results show conserved functions during gynoecium development and novel roles that enrich the knowledge of the SPT gene in fleshy fruits.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143045907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tissue-specific responses of the central carbon metabolism in tomato fruit to low oxygen stress.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf018

Xindan Li, Konstantinos Terzoudis, Maarten L A T M Hertog, Bart M Nicolaï

{"title":"Tissue-specific responses of the central carbon metabolism in tomato fruit to low oxygen stress.","authors":"Xindan Li, Konstantinos Terzoudis, Maarten L A T M Hertog, Bart M Nicolaï","doi":"10.1093/jxb/eraf018","DOIUrl":"https://doi.org/10.1093/jxb/eraf018","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is an important model plant whose fleshy fruit consists of well-differentiated tissues. Recently it was shown that these tissues develop hypoxia during fruit development and ripening. Therefore, we employed a combination of metabolomics and isotopic labeling to investigate the central carbon metabolic response of tomato fruit tissues (columella, septa and mesocarp) to low O2 stress. The concentration and 13C-label enrichment of intermediates from the central carbon metabolism were analyzed using gas chromatography-mass spectrometry. The results showed an increase in glycolytic activity and the initiation of fermentation in response to low O2 conditions. In addition, the up-regulation of the GABA shunt and accumulation of amino acids, alanine and glycine, were observed under low O2 conditions. Notably, tissue specificity was observed at the metabolite level, with concentrations of most metabolites being highest in columella tissue. In addition, there were tissue-specific differences in the central carbon metabolism with the columella exhibiting the highest metabolic activity and sensitivity to the changes in O2 concentration, followed by septa and mesocarp tissues. Our results are consistent with common plant responses and adaptive mechanisms to low O2 stress, while unravelling some tissue-specific differences, increasing our understanding of the intact fruit response to low O2 stress.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA methylation dynamics in the shoot apical meristem. 嫩枝顶端分生组织中 DNA 甲基化的动态变化

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf024

Jie Yang, Frank Johannes

引用次数: 0

A complex interplay between histone variants and DNA methylation.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf030

Alejandro Edera, Leandro Quadrana

引用次数: 0

Nitric oxide as integral element in priming- induced tolerance and plant stress memory.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf033

Gábor Fejes, Tamás Bodor, Réka Szőllősi, Selahattin Kondak, Kinga Kutasi, Vasileios Fotopoulos, Zsuzsanna Kolbert

{"title":"Nitric oxide as integral element in priming- induced tolerance and plant stress memory.","authors":"Gábor Fejes, Tamás Bodor, Réka Szőllősi, Selahattin Kondak, Kinga Kutasi, Vasileios Fotopoulos, Zsuzsanna Kolbert","doi":"10.1093/jxb/eraf033","DOIUrl":"https://doi.org/10.1093/jxb/eraf033","url":null,"abstract":"The beneficial effects of priming technology are aimed at the promotion of growth and development and stress tolerance in plants. Different seed pre-treatment and vegetative priming approaches (osmo-, chemical, physical, hormonal, redox treatments) increase the level of nitric oxide (NO) being an active contributor to growth regulation and defence responses. On the other hand, seed pre-treatment or vegetative priming mainly with the NO donor, sodium nitroprusside (SNP) helps to mitigate different abiotic stresses like salinity, cold, drought, excess metals. The effect of SNP/NO covers the alleviation of stress-specific effects (e.g. reduction of cadmium uptake in case of cadmium loading, improvement of water balance in case of drought), as well as general effects, such as alleviating oxidative stress. Seed pre-treatment or vegetative priming with SNP/NO up-regulates antioxidant enzymes at the activity level and increases the amount of a wide range of non-enzymatic antioxidants. However, due to adverse effects of SNP we urge the testing of other conventional NO donors (e.g. S-nitrosoglutathione) as well as new substances with more favourable properties (e.g. NO-releasing nanomaterials, plasmas, plasma treated liquids, combined donors) as seed pre-treating and vegetative priming materials. This review gives further suggestions on the methodology and future directions of fundamental research in relation to NO-associated pre-treatment and priming.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional regulation of development by SMAX1-LIKE proteins, targets of strigolactone and karrikin/KAI2 ligand signaling.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf027

Sun Hyun Chang, Wesley George, David C Nelson

{"title":"Transcriptional regulation of development by SMAX1-LIKE proteins, targets of strigolactone and karrikin/KAI2 ligand signaling.","authors":"Sun Hyun Chang, Wesley George, David C Nelson","doi":"10.1093/jxb/eraf027","DOIUrl":"https://doi.org/10.1093/jxb/eraf027","url":null,"abstract":"SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-LIKE (SMXL) proteins comprise a family of plant growth regulators that includes downstream targets of the karrikin (KAR)/KAI2 ligand (KL) and strigolactone (SL) signaling pathways. Following the perception of KAR/KL or SL signals by α/β hydrolases, some types of SMXL proteins are polyubiquitinated by an E3 ubiquitin ligase complex containing the F-box protein MORE AXILLARY GROWTH2 (MAX2)/DWARF3 (D3), and proteolyzed. Because SMXL proteins interact with TOPLESS (TPL) and TPL-related (TPR) transcriptional corepressors, SMXL degradation initiates changes in gene expression. This simplified model of SMXL regulation and function in plants must now be revised in light of recent discoveries. It has become apparent that SMXL abundance is not regulated by KAR/KL or SL alone, and that some SMXL proteins are not regulated by MAX2/D3 at all. Therefore, SMXL proteins should be considered signaling hubs that integrate multiple cues. Here we review the current knowledge of how SMXL proteins impose transcriptional regulation of plant development and environmental responses. SMXL proteins can bind DNA directly and interact with transcriptional regulators from several protein families. Multiple mechanisms of downstream genetic control by SMXL proteins have been identified recently that do not involve the recruitment of TPL/TPR, expanding the paradigm of SMXL function.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0