Journal of Experimental Botany最新文献_第4页

Does leaf lifespan affect the regulation of photosynthetic function during natural senescence of leaves in various sorghum cultivars? 不同高粱品种叶片自然衰老过程中叶片寿命对光合功能的调节是否有影响？

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-09 DOI: 10.1093/jxb/eraf195

Han-Yu Wu, Zhi-Quan Liu, Wang-Feng Zhang, Qing-Min Pan, Chuang-Dao Jiang

{"title":"Does leaf lifespan affect the regulation of photosynthetic function during natural senescence of leaves in various sorghum cultivars?","authors":"Han-Yu Wu, Zhi-Quan Liu, Wang-Feng Zhang, Qing-Min Pan, Chuang-Dao Jiang","doi":"10.1093/jxb/eraf195","DOIUrl":"https://doi.org/10.1093/jxb/eraf195","url":null,"abstract":"The regulation of photosynthetic functions during leaf senescence may be associated with leaf lifespan, but how to accurately reveal photosynthetic regulation during senescence based on significant differences in the leaf lifespan remains unresolved. Accordingly, this study evaluated sorghum cultivars with markedly disparate leaf lifespans. WSC62 was characterized by an extended leaf lifespan and earlier initiation of senescence, which resulted in a slower rate of leaf senescence than WSC34. In leaves, having different senescence onset times influenced the comparison of photosynthesis during leaf senescence between the two cultivars. The two cultivars showed significant differences in photosynthetic rate and photosystem II activity based on their senescence initiation timing throughout the leaf lifespan. The senescence rate of WSC62 was slower than that of WSC34. However, differences in the photosynthetic function at corresponding senescence stages were significantly reduced in both cultivars after normalization by the leaf senescence duration. Notably, WSC62 exhibited a lower photosynthetic rate and a higher senescence rate than WSC34. This was further validated by data from four additional sorghum cultivars. Consequently, selecting the onset of senescence based on the leaf lifespan was conducive to revealing variations in photosynthetic function during leaf senescence among sorghum cultivars. Furthermore, normalization by the leaf senescence duration demonstrated that there were patterns related and unrelated to the synergistic interaction between lifespan and senescence in the photosynthetic regulation of senescing leaves. The relationship between the patterns of photosynthetic regulation during leaf senescence and cultivar improvement are also discussed.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Four guidelines to identify bona fide protease-substrate pairs. 鉴定真正蛋白酶底物对的四个准则。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-09 DOI: 10.1093/jxb/eraf194

Shuning Qi, Shamik Mazumdar, Yong Zou, Qun Yang, Simon Stael, Maxence James

{"title":"Four guidelines to identify bona fide protease-substrate pairs.","authors":"Shuning Qi, Shamik Mazumdar, Yong Zou, Qun Yang, Simon Stael, Maxence James","doi":"10.1093/jxb/eraf194","DOIUrl":"https://doi.org/10.1093/jxb/eraf194","url":null,"abstract":"Plant proteases are an important class of enzymes, with proposed involvement in various aspects of the plant life cycle. However, pinpointing authentic protease-substrate interactions remains challenging, which hinders a comprehensive understanding of the biological function of proteases. Moreover, a structured set of guidelines to validate protease substrates is lacking. In this review, we outline a minimum of four key guidelines that, when followed, can confirm the specificity of protease-substrate interaction for proteases that perform limited proteolysis and with specific cleavage sites: (i) the observation of substrate cleavage; (ii) the reduction in substrate cleavage due to protease inhibitors or (iii) genetic mutation of the protease; (iv) a final proof of the specificity of the substrate cleavage site. It is important to emphasize that these guidelines are not universally applicable to all proteases. By creating a set of guidelines, summarizing current findings and proposing future research directions, this review aims to highlight innovative techniques that will improve the specificity and accuracy of protease research and facilitate a deeper understanding of the role of proteases in plant biology.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OsGRF1 and OsGRF2 play unequal redundant roles in regulating leaf vascular bundle formation. OsGRF1和OsGRF2在调节叶片维管束形成过程中发挥着不相等的冗余作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-08 DOI: 10.1093/jxb/eraf193

Ruihan Xu, Jianyu An, Jiali Song, Tiantian Yan, Jieru Li, Xuebin Zhao, Xi'an Shi, Yayi Meng, Chenyu Rong, Ganghua Li, Yanfeng Ding, Chengqiang Ding

{"title":"OsGRF1 and OsGRF2 play unequal redundant roles in regulating leaf vascular bundle formation.","authors":"Ruihan Xu, Jianyu An, Jiali Song, Tiantian Yan, Jieru Li, Xuebin Zhao, Xi'an Shi, Yayi Meng, Chenyu Rong, Ganghua Li, Yanfeng Ding, Chengqiang Ding","doi":"10.1093/jxb/eraf193","DOIUrl":"https://doi.org/10.1093/jxb/eraf193","url":null,"abstract":"Leaf shape and angle are crucial components of plant architecture and substantially influence photosynthetic efficiency and crop productivity. In this study, we found that OsGRF1 (GROWTH-REGULATING FACTOR 1) and OsGRF2 play unequally redundant roles in regulating leaf development. The osgrf1 mutant showed increased leaf angle, and mutations in OsGRF2 further enhanced this effect, although the leaf angle of the osgrf2 mutant remained unchanged. Cell elongation on the adaxial collar side caused an increased leaf angle. rOsGRF1 rOsGRF2 materials, resistant to miR396 cleavage, had reduced leaf angles. Furthermore, due to abnormal vascular bundle development, the osgrf1 osgrf2 mutants showed narrow leaves and twisted flag leaves, which may be associated with the downregulation of the midrib-regulating gene DL (DROOPING LEAF). OsGRF1-GFP and OsGRF2-GFP are localized in leaf primordia and young leaves, which is consistent with their roles in regulating leaf development. osgrf1 osgrf2 double mutants exhibited increased sensitivity to brassinosteroids, with RNA-seq enriching for brassinosteroids-related genes. Since OsGIF3 negatively regulates leaf angle, the OsGRF1-OsGIF3 and OsGRF2-OsGIF3 complexes influence the development of lamina joint. Haplotype analysis showed the artificial selection of OsGRF1. Our findings indicate that OsGRF1/2 negatively regulates leaf angle but positively regulates grain size, which holds promise for applications in agricultural production.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homeodomain transcription factor regulation of leaf serrations in Arabidopsis thaliana. 拟南芥叶片锯齿形的同源结构域转录因子调控。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-08 DOI: 10.1093/jxb/eraf174

Eleanor E Imlay, Hyung-Woo Jeon, Mary E Byrne

{"title":"Homeodomain transcription factor regulation of leaf serrations in Arabidopsis thaliana.","authors":"Eleanor E Imlay, Hyung-Woo Jeon, Mary E Byrne","doi":"10.1093/jxb/eraf174","DOIUrl":"https://doi.org/10.1093/jxb/eraf174","url":null,"abstract":"Arabidopsis thaliana has simple spatulate to obovate shaped leaves, with subtle serrations along the margin. BEL1-like homeodomain transcription factors (BLH), SAWTOOTH1 and SAWTOOTH2, and the class II KNOTTED1-like homeobox (KNOXII) KNAT3 are suppressors of serration outgrowths. Double saw1 saw2 mutants have enhanced serrations and knat3 single mutants have a subtle increase in serrations relative to wild type. However, the relationship between these homeodomain proteins is unclear. Here we show SAW1, SAW2, and KNAT3 have overlapping expression patterns on the adaxial side of the leaf and triple mutants saw1 saw2 knat3 are greatly enhanced relative to the saw1 saw2 double mutant and knat3 single mutant. KNAT3 expressed in the adaxial leaf from the endogenous promoter suppresses the saw1 saw2 knat3 phenotype whereas ectopic expression throughout the leaf or in the abaxial domain enhances the triple mutant serration phenotype. This ectopic KNAT3 activity can be modified by ectopic expression of SAW1. These results demonstrate that the adaxial-abaxial domain specificity of BLH and KNOXII homeodomain proteins has consequences for serration geometry.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Starting strong: development and biomechanics of the seedling-host interaction in European mistletoe (Viscum album). 强启动：欧洲槲寄生幼苗-寄主相互作用的发育和生物力学（Viscum album）。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-07 DOI: 10.1093/jxb/eraf129

Luiza Teixeira-Costa, Lukas Wiese, Thomas Speck, Max D Mylo

{"title":"Starting strong: development and biomechanics of the seedling-host interaction in European mistletoe (Viscum album).","authors":"Luiza Teixeira-Costa, Lukas Wiese, Thomas Speck, Max D Mylo","doi":"10.1093/jxb/eraf129","DOIUrl":"https://doi.org/10.1093/jxb/eraf129","url":null,"abstract":"Attachment to a substrate is fundamental for plant growth and development. This is especially true for species that live either partially or fully off the ground, such as mistletoes, which have developed unique adaptations to anchor themselves securely to host trees from which they draw water and some nutrients. While the mechanical properties of attachment during the adult stages in many plant species have been described, the mechanical principles of the initial developmental stages are rarely investigated. Here, we focus on the parasitic European mistletoe (Viscum album L.) and its attachment to a host plant at the seedling stage. Using a combination of germination experiments, microtomography, histological analysis and biomechanical tests, this work investigates the role of the three key attachment structures involved in this process: seed coat, hypocotyl and holdfast. The viscin layer, a sticky coating on the seed, provides initial adhesion before the growing hypocotyl expands towards the host surface where it flattens and forms a holdfast that strengthens adhesion and aids tissue penetration. Tensile tests revealed that these three attachment structures withstand similar forces in the early stages, considerably higher than the weight of the seedling. Within a few months, the endophytic system interlocked with the host bark, forming a robust connection that not only transports water but also increased the mechanical strength of the structure. This work highlights the fundamental mechanisms of the initial mistletoe-host interaction, which forms the basis of their decades-long relationship.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway. 大麦叶片-叶柄基因Uniculme4对植株结构和茉莉酸途径的多效性影响

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-07 DOI: 10.1093/jxb/eraf068

Elahe Tavakol, Vahid Shariati, Irene Maria Fontana, Marta Binaghi, Hagen Stellmach, Bettina Hause, Gerit Bethke, Hatice Bilgic, Jayanand Boddu, Ron Okagaki, Shane Heinen, Gary J Muehlbauer, Laura Rossini

{"title":"Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway.","authors":"Elahe Tavakol, Vahid Shariati, Irene Maria Fontana, Marta Binaghi, Hagen Stellmach, Bettina Hause, Gerit Bethke, Hatice Bilgic, Jayanand Boddu, Ron Okagaki, Shane Heinen, Gary J Muehlbauer, Laura Rossini","doi":"10.1093/jxb/eraf068","DOIUrl":"https://doi.org/10.1093/jxb/eraf068","url":null,"abstract":"Plant architecture is a key determinant of crop yield, and understanding the genetic basis of its regulation is crucial for crop improvement. BLADE-ON-PETIOLE (BOP) genes are known to play a fundamental role in shaping plant architecture across diverse species. In this study, we demonstrate pleiotropic effects of the barley BOP gene Uniculme4 (Cul4) on various aspects of plant architecture, including plant height, culm diameter, and grain traits. Accordingly, Cul4 is broadly expressed in different tissues and developmental stages. Comparing transcriptome profiles of cul4 mutant and wild-type plants, we uncover a novel link between Cul4 and the jasmonic acid (JA) biosynthetic pathway. Our findings demonstrate that proper Cul4 function is required to repress JA biosynthesis, with cul4 mutants exhibiting increased levels of JA and its precursor 12-oxo-phytodienoic acid. Up-regulation of WRKY and bHLH transcription factors shows JA signalling is also impacted by Cul4. Additionally, our study sheds light on the role of Cul4 in flowering time regulation, potentially through its interaction with florigen-like genes. This research enhances our understanding of the mechanisms and pathways acting downstream of BOP genes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terminomics for plant protease substrate discovery: Achievements, challenges and new opportunities. 术语组学用于植物蛋白酶底物发现：成就、挑战和新机遇。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-04 DOI: 10.1093/jxb/eraf173

Melissa Mantz, Paula Dewes, Pitter F Huesgen

{"title":"Terminomics for plant protease substrate discovery: Achievements, challenges and new opportunities.","authors":"Melissa Mantz, Paula Dewes, Pitter F Huesgen","doi":"10.1093/jxb/eraf173","DOIUrl":"https://doi.org/10.1093/jxb/eraf173","url":null,"abstract":"Plants contain hundreds of proteases that are involved in the regulation of virtually all cellular processes. Some proteases act as molecular shredders, resulting in degradation of their substrates. Others act more like scissors, cutting substrate proteins in limited manner at specific sites to alter their activity, location and function. Such tailored proteoforms share their sequence with the precursor form and sometimes only differ by the new, proteolytically modified polypeptide termini. Identification of protein termini is mandatory for unambiguous identification, but challenging in standard mass spectrometry-based proteomics. Over the last two decades, various methods for the enrichment of N- and C-terminal peptides have been developed to enable proteome-wide characterization. Here we briefly introduce major approaches to protein termini enrichment and review current applications for plant protease substrate identification and profiling of proteolytic cleavage events in vivo. We highlight both successes and limitations and discuss current improvements in sample preparation, data acquisition, mass spectrometry instrumentation and data analysis that promise to increase sensitivity, robustness and ultimately utility of termini-centric proteomics.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of reactive oxygen, nitrogen and sulphur species in the integration of (a)biotic stress signals in legumes. 活性氧、氮和硫在豆科植物生物胁迫信号整合中的作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-04 DOI: 10.1093/jxb/eraf175

Alexander Johansson, Baptiste Sarrette, Alexandre Boscari, Marion Prudent, Véronique Gruber, Renaud Brouquisse, Christophe Jacquet, Clare Gough, Nicolas Pauly

{"title":"The role of reactive oxygen, nitrogen and sulphur species in the integration of (a)biotic stress signals in legumes.","authors":"Alexander Johansson, Baptiste Sarrette, Alexandre Boscari, Marion Prudent, Véronique Gruber, Renaud Brouquisse, Christophe Jacquet, Clare Gough, Nicolas Pauly","doi":"10.1093/jxb/eraf175","DOIUrl":"https://doi.org/10.1093/jxb/eraf175","url":null,"abstract":"Legumes are crops of considerable economic and ecological significance. They are suitable for cultivation in a variety of environments and temperatures. They are able to form a symbiotic relationship with nitrogen-fixing soil bacteria called rhizobia contributing to the enrichment of marginal soils with this essential nutrient, and reduces artificial fertilizer use. Similarly, legumes establish a widespread mutualistic association with soil fungi, involving a reciprocal transfer of nutrients. Global warming is reshaping plant interactions with its environment, exerting profound effects on global agricultural systems. Plants have evolved sensing, signaling, and adaptive molecular mechanisms to respond to (a)biotic stimuli. Reactive oxygen, nitrogen, and sulphur species (RONSS) are key players in stress tolerance mechanisms, and their homeostasis, mainly accomplished by antioxidant enzymes and metabolites, is essential to allow redox signaling while preventing oxidative damage. Here, we review recent findings, highlighting metabolic pathways of RONSS and antioxidants, with emphasis on their functions in signaling and protection in response to (a)biotic constraints in legumes. Special attention is paid to the molecular crosstalk between RONSS in response to multiple stimuli and notably how redox homeostasis adjustment can increase (a)biotic stress tolerance and potentially be exploited to mitigate the negative effects of climate change.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Viral Movement Proteins and Plasmodesmata: turning gatekeepers into gateways. 病毒运动、蛋白质和间连丝：把守门人变成门户。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-04 DOI: 10.1093/jxb/eraf184

Mazen Alazem, Samantha N Nuzzi, Tessa M Burch-Smith

引用次数: 0

The role of stress hormones in regulating tomato resilience and metabolism. 应激激素在调节番茄恢复力和代谢中的作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-04 DOI: 10.1093/jxb/eraf187

Shivangini Jaryal, Sokol Toçilla, Krešimir Šola, Sara Selma García, Alain Goossens

引用次数: 0