Journal of Experimental Botany最新文献_第5页

Acylamino acid-releasing enzyme, a bifunctional protease with a potential role in aging. 酰基氨基酸释放酶（AARE）是一种双功能蛋白酶，在衰老过程中具有潜在作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/eraf169

Sebastian N W Hoernstein, Alessandra A Miniera, Ralf Reski

{"title":"Acylamino acid-releasing enzyme, a bifunctional protease with a potential role in aging.","authors":"Sebastian N W Hoernstein, Alessandra A Miniera, Ralf Reski","doi":"10.1093/jxb/eraf169","DOIUrl":"10.1093/jxb/eraf169","url":null,"abstract":"Acylamino acid-releasing enzyme (AARE) is a highly evolutionary conserved, bifunctional serine protease. In its exopeptidase mode, AARE cleaves N-terminally acetylated or otherwise blocked amino acids from the N-terminus of peptides, and probably even intact proteins. In its endopeptidase mode, AARE cleaves oxidised proteins at internal positions. Although AARE function was discovered 50 years ago and has been biochemically characterized in various organisms, the precise role of this protease in cellular physiology remains elusive. Several other names for AARE do exist in literature, such as acylpeptide hydrolase, acylaminoacyl peptidase, and oxidised protein hydrolase. Recently, the first AARE null mutants have been described in the model moss Physcomitrium patens (Physcomitrella). Comparisons with T-DNA mutants in Arabidopsis thaliana revealed a role for AARE in the timing of the developmental transition from the vegetative to the reproductive state, as well as in the determination of life span. Loss of AARE function was accompanied by a striking increase in oxidised proteins, a hallmark of cellular aging. In mammals, AARE activity is linked to proteasomal function, and dysregulation of AARE function has been observed in different types of cancer and age-related pathologies. Here, we compile the current knowledge on molecular and biological functions of this protease, aiming to derive common roles of AARE in cellular physiology, and potentially in aging, but also highlight differences between species isoforms.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4262-4278"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144009903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balance of chlorophyll synthesis and thylakoid protein quality control influences chloroplast development in var2 mutants. 叶绿素合成平衡和类囊体蛋白质量控制影响var2叶绿体发育。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/eraf177

Peiyi Wang, Lin Zhu, Xiaomin Wang, Yanan Zhai, Jiahui Zhong, Hongchang Zhang, Jingxia Shao, Xiayan Liu, Fei Yu, Yafei Qi

{"title":"Balance of chlorophyll synthesis and thylakoid protein quality control influences chloroplast development in var2 mutants.","authors":"Peiyi Wang, Lin Zhu, Xiaomin Wang, Yanan Zhai, Jiahui Zhong, Hongchang Zhang, Jingxia Shao, Xiayan Liu, Fei Yu, Yafei Qi","doi":"10.1093/jxb/eraf177","DOIUrl":"10.1093/jxb/eraf177","url":null,"abstract":"Filamentous temperature-sensitive H (FtsH) is a major protease for thylakoid protein quality control in photosynthetic organisms. Mutations of the AtFtsH2 subunit in Arabidopsis result in yellow variegated2 (var2) mutants. Genetic screening of ENHANCER OF VARIEGATION (EVR) loci provides new insights into the role of FtsH in chloroplast development beyond the degradation of photodamaged D1 protein. Here, using Arabidopsis, we uncover a novel function of EVR3, previously reported as ETHYLENE-DEPENDENT GRAVITROPISM-DEFICIENT AND YELLOW-GREEN1 (EGY1), in regulating chlorophyll biosynthesis by association with the H subunit of magnesium chelatase (CHLH). Additionally, we identify a new var2 enhancer mutant, evr4-1, caused by a missense mutation in CHLH. The evr4-1 mutant shows a significant decline in accumulation of light-harvesting complexes rather than in photosystem II core proteins, while evr3 evr4 double mutants exhibit synthetic lethal phenotypes accompanied by a drastic reduction in the accumulation of chlorophyll and light-harvesting complexes. Furthermore, disruption of the thylakoid protein sorting pathway mediated by the chloroplast Signal Recognition Particle 54 kDa protein mitigates the chloroplast development defect in var2-4 evr4-1. Our findings underscore the critical role of thylakoid FtsH for thylakoid protein quality control when chlorophyll biosynthesis is disrupted.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4359-4373"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ECLIPSE mediates selective degradation of inner nuclear membrane protein in plants. ECLIPSE介导植物核膜蛋白的选择性降解。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/eraf167

Enrico Calvanese, Min Jia, Olivia Xie, Yangnan Gu

{"title":"ECLIPSE mediates selective degradation of inner nuclear membrane protein in plants.","authors":"Enrico Calvanese, Min Jia, Olivia Xie, Yangnan Gu","doi":"10.1093/jxb/eraf167","DOIUrl":"10.1093/jxb/eraf167","url":null,"abstract":"The inner nuclear membrane (INM) hosts a unique set of membrane proteins essential for nuclear functions. Proteolytic removal of mislocalized or defective membrane proteins is of critical importance for maintaining the homeostasis and integrity of the INM. Previous studies revealed that INM protein degradation depends on a specialized ubiquitin-proteasome system termed INM-associated degradation (INMAD) in plants, requiring the CDC48 complex and the 26S proteasome for membrane protein retrotranslocation and destruction, respectively. However, details of the adaptor proteins that link membrane substrates to the CDC48/proteasome degradation machinery are still lacking in the pathway. Here, we report the discovery of ECLIPSE, a previously uncharacterized protein that may serve as such a molecular bridge in the degradation of the conserved INM protein SUN1. We demonstrate that ECLIPSE physically associates with CDC48 and exhibits strong transcriptional co-regulation with multiple established plant INMAD components. Mechanistically, ECLIPSE may act as an adaptor through its dual-domain architecture: its C-terminal PUB domain mediates direct interaction with CDC48, while its N-terminal ubiquitin-associated domain recognizes ubiquitinated INM substrates. Genetic and biochemical analyses further established that ECLIPSE is required for SUN1 protein degradation in Arabidopsis, supporting its role in the turnover of at least some INM proteins in plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4326-4339"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The CAPE1 peptide confers resistance against bacterial wilt in tomato. CAPE1 肽能增强番茄对细菌性枯萎病的抗性。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/eraf145

Weiqi Zhang, Marc Planas-Marquès, Moyan Liang, Qingshan Zhang, Annemarie Vermeulen, Farnusch Kaschani, Markus Kaiser, Frank L W Takken, Nuria S Coll, Marc Valls

{"title":"The CAPE1 peptide confers resistance against bacterial wilt in tomato.","authors":"Weiqi Zhang, Marc Planas-Marquès, Moyan Liang, Qingshan Zhang, Annemarie Vermeulen, Farnusch Kaschani, Markus Kaiser, Frank L W Takken, Nuria S Coll, Marc Valls","doi":"10.1093/jxb/eraf145","DOIUrl":"10.1093/jxb/eraf145","url":null,"abstract":"Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial diseases for which no effective treatment exists. There is an urgent need to understand the basis of resistance against this pathogen in order to engineer efficient strategies in the field. We previously demonstrated that resistant tomato plants limit bacterial movement in the apoplast and the xylem. As a first step to dissect the underlying mechanisms, we analysed the apoplast proteome upon challenge with R. solanacearum in the susceptible tomato cultivar Marmande and the resistant cultivar Hawaii 7996. Here, we described the xylem proteome in these same cultivars and compared it with the apoplastic proteome, revealing variety-dependent and infection-dependent changes. This proteomic analysis led to the identification of pathogenesis-related 1 (PR1) proteins as highly induced upon infection. Since PR1b was the most abundant PR1 protein in both the apoplast and the xylem, we concentrated on this family member to study the role of PR1s in the interaction between tomato and R. solanacearum. Surprisingly, lack of PR1b resulted in enhanced resistance to R. solanacearum in tomato, which could be due to an up-regulation of homologous genes in a compensatory effect as has been reported before. PR1 processing by an unknown protease in tomato results in the generation of the CAPE peptide. Treatment of tomato plants with the CAPE1 peptide resulted in restriction of R. solanacearum growth, via defence gene reprogramming. Future work in the lab will help determine which tomato secreted proteases cleave PR1s to generate CAPEs.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4340-4358"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hacking the immune system: plant immune protease engineering for crop protection. 破解免疫系统：作物保护用植物免疫蛋白酶工程。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/eraf137

Mariana Schuster, Alexander L Ciattoni, Renier A L van der Hoorn

引用次数: 0

Silenced cutters: mechanisms and effects of protease inhibition in plant-pathogen interactions. 沉默切割器：植物与病原体相互作用中蛋白酶抑制的机制和作用。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/eraf156

Catarina Paiva-Silva, João Proença Pereira, Frederico Marcolino, Andreia Figueiredo, Rita B Santos

{"title":"Silenced cutters: mechanisms and effects of protease inhibition in plant-pathogen interactions.","authors":"Catarina Paiva-Silva, João Proença Pereira, Frederico Marcolino, Andreia Figueiredo, Rita B Santos","doi":"10.1093/jxb/eraf156","DOIUrl":"10.1093/jxb/eraf156","url":null,"abstract":"Proteases are essential enzymes in plants that play multiple roles in immunity, including molecular recognition, programmed cell death, and the degradation of pathogen proteins. During plant-pathogen interactions, both organisms have evolved mechanisms to regulate protease activity. Plants produce specific inhibitors to prevent excessive or harmful proteolysis, while pathogens counteract these defences by deploying molecules that block proteases and weaken plant immunity. Despite significant progress in understanding protease function, many regulatory mechanisms remain unexplored. This review examines the roles of endoproteases in plant responses to biotic stress and the diverse strategies employed by both plants and pathogens to modulate their activity. We discuss known protease inhibition mechanisms and highlight emerging methodologies that offer new insights into protease regulation. Additionally, we explore biotechnological applications, including genetic engineering and chemical inhibitors, aimed at enhancing plant resistance to pathogens. By integrating current knowledge with innovative research tools, we can uncover novel protease regulatory pathways and develop new strategies to improve plant resilience. Understanding these mechanisms not only advances fundamental plant biology but also holds potential for sustainable agricultural practices in the face of evolving pathogen threats.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"4248-4261"},"PeriodicalIF":5.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12485229/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RD21-like proteases: key effector hubs in plant-pathogen interactions. rd21样蛋白酶：植物与病原体相互作用的关键效应中心。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-10-01 DOI: 10.1093/jxb/erae496

Jie Huang, Renier A L van der Hoorn

引用次数: 0

Structural and functional comparison of hemoglobin Glb2-1 of Lotus japonicus with Glb1-1 and leghemoglobins. 莲子血红蛋白Glb2-1与Glb1-1及豆血红蛋白的结构和功能比较。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-30 DOI: 10.1093/jxb/eraf434

Rosa M Esquinas-Ariza, Irene Villar, Samuel Minguillón, Ángel Zamarreño, Carmen Pérez-Rontomé, Brandon J Reeder, Niels Sandal, Deng Yan, José M García-Mina, Deqiang Duanmu, Marta Martínez-Júlvez, Manuel Becana

{"title":"Structural and functional comparison of hemoglobin Glb2-1 of Lotus japonicus with Glb1-1 and leghemoglobins.","authors":"Rosa M Esquinas-Ariza, Irene Villar, Samuel Minguillón, Ángel Zamarreño, Carmen Pérez-Rontomé, Brandon J Reeder, Niels Sandal, Deng Yan, José M García-Mina, Deqiang Duanmu, Marta Martínez-Júlvez, Manuel Becana","doi":"10.1093/jxb/eraf434","DOIUrl":"https://doi.org/10.1093/jxb/eraf434","url":null,"abstract":"The legume Lotus japonicus expresses nine hemoglobins, including leghemoglobins (Lbs), class 1 phytoglobin (Glb1-1), and an unusual phytoglobin (Glb2-1). Quantitative PCR, proteomics, and plant mutant analyses indicate that Glb2-1 is mainly present in nodules without replacing Lb function, but is also in roots and photosynthetic tissues. Comparison of hormonal profiles of the knock-out mutants glb1-1, glb2-1, and glb1-1/2-1 reveals that Glb1-1 and Glb2-1 have distinct functions. The increase of salicylic acid in the leaves of glb1-1 reveals a role of Glb1-1 in the defense response, which was corroborated by accumulation of pipecolic acid, a metabolite involved in plant immunity. In contrast, the decrease of bioactive jasmonoyl-isoleucine in glb2-1 is consistent with a role of Glb2-1 in the plant's reproductive stage. The mutants also showed changes in cytokinins, gibberellins, and polyamines, but without clear distinctive patterns. The crystal structure of Glb2-1 was determined to 1.6 Å resolution and compared with those of soybean Lba and Arabidopsis Glb1. In combination with mutant versions of Glb2-1, residues Tyr31, His64, and Cys65 were identified as critical for O2-binding stability. Spectral changes in heme coordination when Tyr31 is substituted for Phe highlights the importance of the residue at the B10 position for Lb and Glb function.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tRNA-gRNA multiplexing system for CRISPR genome editing in Marchantia polymorpha. 用于多形地豆CRISPR基因组编辑的tRNA-gRNA复用系统

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-30 DOI: 10.1093/jxb/eraf433

Eftychios Frangedakis, Nataliya E Yelina, Satish Kumar Eeda, Facundo Romani, Alexandros Fragkidis, Jim Haseloff, Julian M Hibberd

{"title":"A tRNA-gRNA multiplexing system for CRISPR genome editing in Marchantia polymorpha.","authors":"Eftychios Frangedakis, Nataliya E Yelina, Satish Kumar Eeda, Facundo Romani, Alexandros Fragkidis, Jim Haseloff, Julian M Hibberd","doi":"10.1093/jxb/eraf433","DOIUrl":"https://doi.org/10.1093/jxb/eraf433","url":null,"abstract":"The liverwort Marchantia polymorpha is a widely used model organism for studying land plant biology, which has also proven to be a promising testbed for bioengineering. CRISPR/Cas9 technology has emerged as a transformative tool for precise genome modifications in M. polymorpha. However, a robust method for the simultaneous expression of multiple gRNAs, which is crucial for enhancing the versatility of CRISPR/Cas9-based genome editing, has yet to be fully developed. In this study, we introduce an adaptation from the OpenPlant kit CRISPR/Cas9 tools, that facilitates expression of multiple gRNAs from a single transcript through incorporation of tRNA sequences. The ability to deliver multiple gRNAs simultaneously, significantly improves the capacity and scalability of genome editing in M. polymorpha. Additionally, by combining this vector system with a simplified and optimized protocol for thallus transformation, we further streamline the generation of CRISPR/Cas9 mutants in M. polymorpha. The resulting gene-editing system offers a multipurpose, time-saving and straightforward tool for advancing functional genomics in M. polymorpha, enabling more comprehensive genetic modifications and genome engineering.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145199603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription factor CsDREB28 regulates sugar transporters CsSWEET17 and CsSWEET15 to modulate cold resistance and plant growth in tea plants. 转录因子CsDREB28调控糖转运蛋白CsSWEET17和CsSWEET15调控茶树的抗寒性和植株生长。

IF 5.7 2区生物学

Journal of Experimental Botany Pub Date : 2025-09-30 DOI: 10.1093/jxb/eraf430

Jing Peng, Yedie Wu, Enbei Liu, Jie Wang, Nana Li, Xinyuan Hao, Changqing Ding, Xinchao Wang, Lu Wang

{"title":"Transcription factor CsDREB28 regulates sugar transporters CsSWEET17 and CsSWEET15 to modulate cold resistance and plant growth in tea plants.","authors":"Jing Peng, Yedie Wu, Enbei Liu, Jie Wang, Nana Li, Xinyuan Hao, Changqing Ding, Xinchao Wang, Lu Wang","doi":"10.1093/jxb/eraf430","DOIUrl":"https://doi.org/10.1093/jxb/eraf430","url":null,"abstract":"Cold stress limits the geographical distribution of tea plants (Camellia sinensis) and negatively affects tea quality and yield. Sugar plays an important role in the cold stress response of tea plants, and CsSWEET17 is a plasma membrane-localized sugar transporter that regulates plant growth and freezing resistance by mediating sugar allocation. In this study, we identified the upstream regulatory factor CsDREB28 of CsSWEET17, an ERF-associated amphiphilic repression protein strongly induced by cold stress that directly inhibits the transcription of CsSWEET17 under both normal and cold stress conditions. Transient silencing of CsDREB28 enhanced the freezing resistance of tea plants by upregulating the expression of the C-repeat-binding (CBF) transcriptional pathway and CsSWEET17 and promoting the accumulation of soluble sugars. Heterologous overexpression of CsDREB28 in Arabidopsis not only significantly reduced freezing resistance but also accelerated leaf senescence, inhibited plant growth, and decreased the seed-setting rate. Further research has shown that CsDREB28 negatively regulates CsSWEET15, a reproductive organ-specific gene, and its Arabidopsis homolog, AtSWEET15, which plays an important role in reproductive growth. Overall, our findings revealed that CsDREB28 inhibits freezing resistance and plant growth by directly transcriptionally inhibiting CsSWEET15/17 to mediate sugar transport and distribution in tea plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0