Journal of Experimental Botany最新文献_第5页

CNGC15 and DMI1 ion channel gating in nuclear calcium signaling: opening new questions and closing controversies. 核钙通道中的 CNGC15-DMI1 门控：提出新问题，结束争论。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae352

Catherine N Jacott, Pablo Del Cerro

引用次数: 0

Linear β-1,2-glucans trigger immune hallmarks and enhance disease resistance in plants. 线性β-1,2-葡聚糖可触发免疫标志并增强植物的抗病性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae368

María Fuertes-Rabanal, Asier Largo-Gosens, Alicia Fischer, Kristina S Munzert, Cristian Carrasco-López, Andrea Sánchez-Vallet, Timo Engelsdorf, Hugo Mélida

{"title":"Linear β-1,2-glucans trigger immune hallmarks and enhance disease resistance in plants.","authors":"María Fuertes-Rabanal, Asier Largo-Gosens, Alicia Fischer, Kristina S Munzert, Cristian Carrasco-López, Andrea Sánchez-Vallet, Timo Engelsdorf, Hugo Mélida","doi":"10.1093/jxb/erae368","DOIUrl":"10.1093/jxb/erae368","url":null,"abstract":"Immune responses in plants are triggered by molecular patterns or elicitors, recognized by plant pattern recognition receptors. Such molecular patterns are the consequence of host-pathogen interactions, and the response cascade activated after their perception is known as pattern-triggered immunity (PTI). Glucans have emerged as key players in PTI, but the ability of certain glucans to stimulate defensive responses in plants remains understudied. This work focused on identifying novel glucan oligosaccharides as molecular patterns. The ability of various microorganism-derived glucans to trigger PTI responses was tested, revealing that specific microbial-derived molecules, such as short linear β-1,2-glucans, trigger this response in plants by increasing the production of reactive oxygen species (ROS), mitogen-activated protein kinase phosphorylation, and differential expression of defence-related genes in Arabidopsis thaliana. Pre-treatments with β-1,2-glucan trisaccharide (B2G3) improved Arabidopsis defence against bacterial and fungal infections in a hypersusceptible genotype. The knowledge generated was then transferred to the monocotyledonous model species maize and wheat, demonstrating that these plants also respond to β-1,2-glucans, with increased ROS production and improved protection against fungal infections following B2G3 pre-treatments. In summary, as with other β-glucans, plants perceive β-1,2-glucans as warning signals which stimulate defence responses against phytopathogens.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7337-7350"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of high purity capsanthin and capsorubin through synthetic metabolic engineering in carrot germplasm. 通过合成代谢工程培育高纯度、高产量的辣椒素和辣椒红素胡萝卜种质。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae351

Yuan-Jie Deng, Ao-Qi Duan, Hui Liu, Zhi-Sheng Xu, Ai-Sheng Xiong

{"title":"Generation of high purity capsanthin and capsorubin through synthetic metabolic engineering in carrot germplasm.","authors":"Yuan-Jie Deng, Ao-Qi Duan, Hui Liu, Zhi-Sheng Xu, Ai-Sheng Xiong","doi":"10.1093/jxb/erae351","DOIUrl":"10.1093/jxb/erae351","url":null,"abstract":"Capsanthin and capsorubin are red κ-xanthophylls exclusively found in a handful of other plant species. Currently, capsanthin and capsorubin are extracted from red pepper (Capsicum annuum L.). Here, high purity production of capsanthin and capsorubin was achieved in carrot (Daucus carota L.) taproot by a synthetic metabolic engineering strategy. Expression of a capsanthin-capsorubin synthase gene (CaCCS) from pepper resulted in dominant production of capsanthin, whereas expression of a LiCCS gene from tiger lily (Lilium lancifolium Thunb.) resulted in production of both capsanthin and capsorubin in carrot taproot. The highest content of capsanthin and capsorubin was obtained in LiC-1 carrot taproot hosting the LiCCS gene. Co-expression of DcBCH1 with CCS could improve the purity of capsanthin and capsorubin by eliminating the non-target carotenoids (e.g. α-carotene and β-carotene). The highest purity of capsanthin and capsorubin was obtained in BLiC-1 carrot taproot hosting DcBCH1+LiCCS genes, 91.10% of total carotenoids. The non-native pigments were esterified partially and stored in the globular chromoplast of carrot taproot. Our results demonstrated the use of carrot taproot as green factories for high purity production of capsanthin and capsorubin. The capsanthin/capsorubin carrot germplasms are also valuable materials for breeding colorful carrots cultivars.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7202-7216"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Model forms for triose phosphate utilization-limited photosynthetic rates: implications for estimation of photorespiratory carbon export and the contribution of mesophyll conductance to photosynthesis. 更正：磷酸三糖利用受限光合速率的模型形式：对估计光呼吸碳输出和叶绿体中层传导对光合作用的贡献的影响。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae470

引用次数: 0

Lemon zinc finger protein ClSUP induces accumulation of reactive oxygen species and inhibits citrus yellow vein-clearing virus infection via interactions with ClDOF3.4. 柠檬锌指蛋白 ClSUP 通过与 ClDOF3.4 相互作用，诱导活性氧积累并抑制柑橘黄脉清病毒感染。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae361

Ping Liao, Ting Zeng, Yuan Chen, Dong-Dong Ding, Chang-Yong Zhou, Yan Zhou

{"title":"Lemon zinc finger protein ClSUP induces accumulation of reactive oxygen species and inhibits citrus yellow vein-clearing virus infection via interactions with ClDOF3.4.","authors":"Ping Liao, Ting Zeng, Yuan Chen, Dong-Dong Ding, Chang-Yong Zhou, Yan Zhou","doi":"10.1093/jxb/erae361","DOIUrl":"10.1093/jxb/erae361","url":null,"abstract":"Citrus yellow vein-clearing virus (Potexvirus citriflavivenae; CYVCV) is an increasing threat to citrus cultivation. Notably, the role of zinc finger proteins (ZFPs) in mediating viral resistance in citrus plants is unclear. In this study, we demonstrated that ZFPs ClSUP and ClDOF3.4 enhanced citrus defense responses against CYVCV in Eureka lemon (Citrus limon 'Eureka'). ClSUP interacted with the coat protein (CP) of CYVCV to reduce CP accumulation and inhibited its silencing suppressor function. Overexpression of CISUP triggered reactive oxygen species (ROS) and salicylic acid (SA) pathways, and enhanced resistance to CYVCV infection. In contrast, ClSUP silencing resulted in increased CP accumulation and down-regulated ROS and SA-related genes. ClDOF3.4 interacted with ClSUP to facilitate its interactions with CP. Furthermore, ClDOF3.4 synergistically regulated the accumulation of ROS and SA with ClSUP and accelerated down-regulation of CP accumulation. Transgenic plants co-expressing ClSUP and ClDOF3.4 significantly decreased the CYVCV. These findings provide a new reference for understanding the interaction mechanism between the host and CYVCV.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7300-7316"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photosynthesis and photoprotection in top leaves respond faster to irradiance fluctuations than bottom leaves in a tomato canopy. 在番茄冠层中，顶部叶片的光合作用和光保护对辐照度波动的反应比底部叶片快。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae357

Bingjie Shao, Yuqi Zhang, Elena Vincenzi, Sarah Berman, Silvere Vialet-Chabrand, Leo F M Marcelis, Tao Li, Elias Kaiser

{"title":"Photosynthesis and photoprotection in top leaves respond faster to irradiance fluctuations than bottom leaves in a tomato canopy.","authors":"Bingjie Shao, Yuqi Zhang, Elena Vincenzi, Sarah Berman, Silvere Vialet-Chabrand, Leo F M Marcelis, Tao Li, Elias Kaiser","doi":"10.1093/jxb/erae357","DOIUrl":"10.1093/jxb/erae357","url":null,"abstract":"Accounting for the dynamic responses of photosynthesis and photoprotection to naturally fluctuating irradiance can improve predictions of plant performance in the field, but the variation of these dynamics within crop canopies is poorly understood. We conducted a detailed study of dynamic and steady-state photosynthesis, photoprotection, leaf pigmentation, and stomatal anatomy in four leaf layers (100, 150, 200, and 250 cm from the floor) of a fully grown tomato (Solanum lycopersicum cv. Foundation) canopy in a greenhouse. We found that leaves at the top of the canopy exhibited higher photosynthetic capacity and slightly faster photosynthetic induction compared with lower-canopy leaves, accompanied by higher stomatal conductance and a faster activation of carboxylation and linear electron transport capacities. In upper-canopy leaves, non-photochemical quenching showed faster induction and relaxation after increases and decreases in irradiance, allowing for more effective photoprotection in these leaves. Despite these observed differences in transient responses between leaf layers, steady-state rather than dynamic photosynthesis traits were more influential for predicting photosynthesis under fluctuating irradiance. Also, a model analysis revealed that time-averaged photosynthesis under fluctuating irradiance could be accurately predicted by one set of Rubisco activation/deactivation parameters across all four leaf layers, thereby greatly simplifying future modelling efforts of whole-canopy photosynthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7217-7236"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel mechanisms of strigolactone-induced DWARF14 degradation in Arabidopsis thaliana. 拟南芥中三氯内酯诱导 DWARF14 降解的新机制

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae365

Elena Sánchez Martín-Fontecha, Francesca Cardinale, Marco Bürger, Cristina Prandi, Pilar Cubas

{"title":"Novel mechanisms of strigolactone-induced DWARF14 degradation in Arabidopsis thaliana.","authors":"Elena Sánchez Martín-Fontecha, Francesca Cardinale, Marco Bürger, Cristina Prandi, Pilar Cubas","doi":"10.1093/jxb/erae365","DOIUrl":"10.1093/jxb/erae365","url":null,"abstract":"In angiosperms, the strigolactone receptor is the α/β hydrolase DWARF14 (D14) that, upon strigolactone binding, undergoes conformational changes, triggers strigolactone-dependent responses, and hydrolyses strigolactones. Strigolactone signalling involves the formation of a complex between strigolactone-bound D14, the E3-ubiquitin ligase SCFMAX2, and the transcriptional corepressors SMXL6/7/8, which become ubiquitinated and degraded by the proteasome. Strigolactone also destabilizes the D14 receptor. The current model proposes that D14 degradation occurs after ubiquitination of the SMXLs via SCFMAX2 and proteasomal degradation. Using fluorescence and luminescence assays on transgenic lines expressing D14 fused to GREEN FLUORESCENT PROTEIN or LUCIFERASE, we showed that strigolactone-induced D14 degradation may also occur independently of SCFMAX2 and/or SMXL6/7/8 through a proteasome-independent mechanism. Furthermore, strigolactone hydrolysis was not essential for triggering either D14 or SMXL7 degradation. The activity of mutant D14 proteins predicted to be non-functional for strigolactone signalling was also examined, and their capability to bind strigolactones in vitro was studied using differential scanning fluorimetry. Finally, we found that under certain conditions, the efficiency of D14 degradation was not aligned with that of SMXL7 degradation. These findings indicate a more complex regulatory mechanism governing D14 degradation than previously anticipated and provide novel insights into the dynamics of strigolactone signalling in Arabidopsis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7145-7159"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142086055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex determination in bryophytes: current state of the art. 苔藓植物的性别鉴定技术现状。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae347

Djordje Božović, Mingai Li, Aneta D Sabovljević, Marko S Sabovljević, Claudio Varotto

引用次数: 0

Post-translational modifications control the signal at the crossroads of plant-pathogen interactions. 翻译后修饰在植物与病原体相互作用的十字路口控制信号

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae358

Kamlesh Kumar Soni, Kishan Gurjar, Aastha Ranjan, Shashank Sinha, Moumita Srivastava, Vivek Verma

{"title":"Post-translational modifications control the signal at the crossroads of plant-pathogen interactions.","authors":"Kamlesh Kumar Soni, Kishan Gurjar, Aastha Ranjan, Shashank Sinha, Moumita Srivastava, Vivek Verma","doi":"10.1093/jxb/erae358","DOIUrl":"10.1093/jxb/erae358","url":null,"abstract":"The co-evolution of plants and pathogens has enabled them to 'outsmart' each other by promoting their own defence responses and suppressing those of the other. While plants are reliant on their sophisticated immune signalling pathways, pathogens make use of effector proteins to achieve the objective. This entails rapid regulation of underlying molecular mechanisms for prompt induction of associated signalling events in both plants as well as pathogens. The past decade has witnessed the emergence of post-translational modification (PTM) of proteins as a key a factor in modulating cellular responses. The ability of PTMs to expand the functional diversity of the proteome and induce rapid changes at the appropriate time enables them to play crucial roles in the regulation of plant-pathogen interactions. Therefore, this review will delve into the intricate interplay of five major PTMs involved in plant defence and pathogen countermeasures. We discuss how plants employ PTMs to fortify their immune networks, and how pathogen effectors utilize/target host modification systems to gain entry into plants and cause disease. We also emphasize the need for identification of novel PTMs and propose the use of PTM pathways as potential targets for genome editing approaches.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6957-6979"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142036025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEMBRANE PROTEIN 1 encoding an amino acid transporter confers resistance to blast fungus and leaf-blight bacterium in rice. 水稻氨基酸转运体 OsMP1 是抗稻瘟病菌和叶枯病菌的数量性状基因座。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae350

Tingting Jiang, Ning Huang, Zhixue Wang, Jiawen Li, Lu Ma, Xinying Wang, Lingtong Shen, Ying Zhang, Yao Yu, Wei Wang, Yunxin Fan, Kunquan Liu, Zhi Zhao, Ziwei Xiong, Qisheng Song, Haijuan Tang, Hongsheng Zhang, Yongmei Bao

{"title":"MEMBRANE PROTEIN 1 encoding an amino acid transporter confers resistance to blast fungus and leaf-blight bacterium in rice.","authors":"Tingting Jiang, Ning Huang, Zhixue Wang, Jiawen Li, Lu Ma, Xinying Wang, Lingtong Shen, Ying Zhang, Yao Yu, Wei Wang, Yunxin Fan, Kunquan Liu, Zhi Zhao, Ziwei Xiong, Qisheng Song, Haijuan Tang, Hongsheng Zhang, Yongmei Bao","doi":"10.1093/jxb/erae350","DOIUrl":"10.1093/jxb/erae350","url":null,"abstract":"Amino acid transporters (AATs) have been shown to be involved in immune responses during plant-pathogen interactions; however, the molecular mechanism by which they function in this process remains unclear. Here, we used a joint analysis of a genome-wide association study and quantitative trait locus (QTL) mapping to identify MEMBRANE PROTEIN 1, which acts as a QTL in rice against blast fungus. Heterogeneous expression of OsMP1 in yeast supported its function in transporting a wide range of amino acids, including Thr, Ser, Phe, His, and Glu. OsMP1 could also mediate 15N-Glu efflux and influx in Xenopus oocyte cells. The expression of OsMP1 was significantly induced by Magnaporthe oryzae in the resistant rice landrace Heikezijing, whereas no such induction was observed in the susceptible landrace Suyunuo. Overexpressing OsMP1 in Suyunuo enhanced disease resistance to blast fungus and leaf blight bacterium without resulting in a yield penalty. In addition, the overexpression of OsMP1 led to increased accumulation of Thr, Ser, Phe, and His in the leaves and this contributed to the reduced disease susceptibility, which was associated with up-regulation of the jasmonic acid pathway. Our results demonstrate the important role of OsMP1 in disease resistance in rice and provide a potential target for breeding more resistant cultivars without reducing yield.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7284-7299"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142017692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0