Journal of Experimental Botany最新文献_第5页

PgMYB96 enhances Physalis grisea high temperature tolerance by activating trithorax-like factor WD REPEAT CONTAINING5b. PgMYB96通过激活含有5b的三胸样因子WD REPEAT增强稻瘟病菌的高温耐受性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-14 DOI: 10.1093/jxb/eraf097

Guanzhuo Kong, Hong Li, Jintao Zheng, Yaru Zhao, Qiaofang Shi, Xiaochun Zhao, Yihe Yu

{"title":"PgMYB96 enhances Physalis grisea high temperature tolerance by activating trithorax-like factor WD REPEAT CONTAINING5b.","authors":"Guanzhuo Kong, Hong Li, Jintao Zheng, Yaru Zhao, Qiaofang Shi, Xiaochun Zhao, Yihe Yu","doi":"10.1093/jxb/eraf097","DOIUrl":"https://doi.org/10.1093/jxb/eraf097","url":null,"abstract":"Under the general trend of global warming, high temperature stress (HTS) is an increasingly grievous challenge to the normal growth and development of crops. Exposure to high temperature (42°C) during the growth and development stages of Physalis grisea can result in the breakdown of antimicrobials, sterility of pollen, and diminished yields. In this study, trithorax-like factor PgWDR5b was functionally analysed in response to high temperature stress in P. grisea. PgWDR5b expression was enhanced through treatments with HTS and abscisic acid (ABA), PgWDR5b promoted the level of expression of downstream ABA synthesis genes after HTS, and positively contributes to tolerance to high temperature stress in P. grisea. In addition, the transcription factor PgMYB96 binds the promoter of PgWDR5b. Silencing both PgWDR5b and PgMYB96 reduced the high temperature tolerance of the P. grisea, as well as the synthesis genes for ABA showed decreased expression while the catabolic genes had increased levels of expression. The results of overexpression assay were contrary. Furthermore, ABA directly activates PgWDR5b expression. These results collectively suggest that PgMYB96 can both regulate PgWDR5b expression by affecting ABA synthesis and directly activate PgWDR5b transcription. Hence, PgWDR5b can participate in the high temperature stress response of P. grisea through the metabolic pathway of ABA and establishes a positive feedback regulatory mechanism with ABA.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The zinc finger transcription factor MtSTOP1 modulates aluminum resistance and low pH tolerance in Medicago truncatula. 锌指转录因子MtSTOP1调控短叶紫花苜蓿的铝抗性和低pH耐受性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/eraf112

Dehui Jin, Jinlong Chen, Chengcheng Yan, Xiaoqing Liu, Yujie Lin, Zhen Li, Zhenfei Guo, Yang Zhang

{"title":"The zinc finger transcription factor MtSTOP1 modulates aluminum resistance and low pH tolerance in Medicago truncatula.","authors":"Dehui Jin, Jinlong Chen, Chengcheng Yan, Xiaoqing Liu, Yujie Lin, Zhen Li, Zhenfei Guo, Yang Zhang","doi":"10.1093/jxb/eraf112","DOIUrl":"https://doi.org/10.1093/jxb/eraf112","url":null,"abstract":"In acidic soils, aluminum (Al) is a primary factor that inhibits plant growth. SENSITIVE-TO-PROTON-RHIZOTOXICITY1 (STOP1), which is accumulated in the nucleus in response to Al or low pH, regulates multiple downstream genes to counteract Al toxicity and acid stress. Here, we discovered four STOP1-like proteins in Medicago truncatula, which are localized in the nucleus and display transcriptional activity. Among them, the expression of MtSTOP1 (the ortholog of AtSTOP1) and MtSTOP2 is slightly induced by Al in the root tips. CRISPR/Cas9-mediated knockout of MtSTOP1 resulted in increased Al and low pH sensitivity. Transcriptomic analysis revealed 110 genes that were differentially downregulated in the Mtstop1 compared to the wild-type under both pH 5.0 and pH 5.0 with Al. qRT-PCR analysis confirmed that MtSTOP1 regulates the expression of several Al-induced genes and MtSTOP2. The mutation of MtSTOP4 significantly decreases the expression of specific Al tolerance genes and compromises Al tolerance, yet less severely than the mutation of MtSTOP1. Furthermore, the double mutant Mtstop1Mtstop4 did not exhibit increased Al sensitivity compared to Mtstop1 alone, suggesting that MtSTOP1 and MtSTOP4 likely function in a cooperative rather than additive manner. Additionally, we found that the C-terminal of F-box protein MtRAE1 interacts with both MtSTOP1 and MtSTOP4. Our study offers significant insights into the transcriptional regulatory mechanisms that respond to Al and acid stress in M. truncatula.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in breeding for enhanced iron and zinc concentrations in common bean in eastern Africa. 东非普通豆铁锌富集育种研究进展。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/eraf009

Paul M Kimani

{"title":"Advances in breeding for enhanced iron and zinc concentrations in common bean in eastern Africa.","authors":"Paul M Kimani","doi":"10.1093/jxb/eraf009","DOIUrl":"10.1093/jxb/eraf009","url":null,"abstract":"Micronutrient malnutrition is one of the most serious health challenges facing vast sectors of the population of Africa, particularly resource-poor women and children. The main deficiencies include iron (Fe), zinc (Zn), and vitamin A. Plant breeding has frequently been advocated as the most sustainable strategy to provide varieties of different food crop species with enhanced micronutrient density to combat the global hidden hunger problem which affects >2 billion people. However, there are few research programmes which have implemented this approach, from concept stage to finished products, which can be widely disseminated and commercialized to create meaningful impact. The east African bean biofortification programme offers a case study of such a programme. The aim of this programme was to develop well-adapted, high-yielding, Fe- and Zn-rich bush and climbing bean cultivars and agronomic approaches that enhance expression of the high mineral trait. The objective of this review is to provide a synthesis of the progress made in the last 22 years, with a focus on genetic diversity, inheritance, bioavailability of Fe and Zn, and cooking quality, as well as to identify research gaps and suggest future directions.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1390-1407"},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11906304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing photosynthetic phosphorus use efficiency through coordination of leaf phosphorus fractions, allocation, and anatomy during soybean domestication. 在大豆驯化过程中，通过协调叶片磷的组分、分配和解剖，提高光合作用磷的利用效率。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/erae427

Geng Chen, Qiu-Xia Ran, Cai Wang, Jiayin Pang, Ming-Jian Ren, Zheng-Yu Wang, Jin He, Hans Lambers

{"title":"Enhancing photosynthetic phosphorus use efficiency through coordination of leaf phosphorus fractions, allocation, and anatomy during soybean domestication.","authors":"Geng Chen, Qiu-Xia Ran, Cai Wang, Jiayin Pang, Ming-Jian Ren, Zheng-Yu Wang, Jin He, Hans Lambers","doi":"10.1093/jxb/erae427","DOIUrl":"10.1093/jxb/erae427","url":null,"abstract":"Soybean domestication has significantly changed key agronomic traits, yet its impact on leaf photosynthetic phosphorus use efficiency (PPUE) and its underlying traits remains poorly known. Further information on this would be important to increase soybean P use efficiency. To address this gap, 48 soybean accessions (16 wild relatives, 16 landraces, and 16 cultivars) were used to compare leaf anatomical traits, foliar chemical P fractions, P allocation, and PPUE under two P levels. The results showed that the cultivars had higher area-based and mass-based photosynthesis rates, PPUE, metabolite P concentration, and its percentage of leaf total P, as well as a greater percentage of lipid P, nucleic acid P, and residual P. Conversely, wild relatives tended to have higher leaf P concentration, palisade:spongy thickness ratio, and concentrations of inorganic P, nucleic acid P, lipid P, and residual P. PPUE was negatively correlated with leaf inorganic P concentration and its percentage relative to leaf total P, while it was positively correlated with the concentration and percentage of metabolite P. We concluded that soybean domestication increased PPUE, as a result of both increased photosynthesis rate and decreased leaf P concentration; domestication reduced the palisade:spongy thickness ratio coupled with increased allocation of P to P-containing metabolites, thereby contributing to faster photosynthesis and higher PPUE. This study sheds light on the significance of leaf P allocation and anatomical traits affecting PPUE during soybean domestication, offering a mechanistic understanding to further enhance soybean P use efficiency.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1446-1457"},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142467247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association studies and transcriptomics reveal mechanisms explaining the diversity of wheat root responses to nutrient availability. 全基因组关联研究和转录组学揭示了解释小麦根系对养分供应反应多样性的机制。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/erae141

Suhaib Ahmad, Hafiza Madeeha Khan, Amjad Nawaz, Muhammad Abdul Samad, Huikyong Cho, Hira Sarfraz, Yasir Aziz, Hatem Rouached, Zaigham Shahzad

{"title":"Genome-wide association studies and transcriptomics reveal mechanisms explaining the diversity of wheat root responses to nutrient availability.","authors":"Suhaib Ahmad, Hafiza Madeeha Khan, Amjad Nawaz, Muhammad Abdul Samad, Huikyong Cho, Hira Sarfraz, Yasir Aziz, Hatem Rouached, Zaigham Shahzad","doi":"10.1093/jxb/erae141","DOIUrl":"10.1093/jxb/erae141","url":null,"abstract":"Nutrient availability profoundly influences plant root system architecture, which critically determines crop productivity. While Arabidopsis has provided important insights into the genetic responses to nutrient deficiency, translating this knowledge to crops, particularly wheat, remains a subject of inquiry. Here, examining a diverse wheat population under varying nitrogen (N), phosphorus (P), potassium (K), and iron (Fe) levels, we uncover a spectrum of root responses, spanning from growth inhibition to stimulation, highlighting genotype-specific strategies. Furthermore, we reveal a nuanced interplay between macronutrient deficiency (N, P, and K) and Fe availability, emphasizing the central role of Fe in modulating root architecture. Through genome-wide association mapping, we identify 11 quantitative trait loci underlying root traits under varying nutrient availabilities, including homologous genes previously validated in Arabidopsis, supporting our findings. In addition, utilizing transcriptomics, reactive oxygen species (ROS) imaging, and antioxidant treatment, we uncover that wheat root growth inhibition by nutrient deficiency is attributed to ROS accumulation, akin to the role of ROS in governing Arabidopsis root responses to nutrient deficiency. Therefore, our study reveals the conservation of molecular and physiological mechanisms between Arabidopsis and wheat to adjust root growth to nutrient availability, paving the way for targeted crop improvement strategies aimed at increasing nutrient use efficiency.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1458-1472"},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140318448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root morphology, exudate patterns, and mycorrhizal symbiosis are determinants to improve phosphorus acquisition in alfalfa. 根系形态、分泌物模式和菌根共生关系是苜蓿提高磷吸收的决定因素。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/eraf107

Jing-Wei Fan, Mei Chen, Fuping Tian, Rui Yao, Nan-Nan Qin, Wen-Hua Wu, Neil C Turner, Feng-Min Li, Yan-Lei Du

{"title":"Root morphology, exudate patterns, and mycorrhizal symbiosis are determinants to improve phosphorus acquisition in alfalfa.","authors":"Jing-Wei Fan, Mei Chen, Fuping Tian, Rui Yao, Nan-Nan Qin, Wen-Hua Wu, Neil C Turner, Feng-Min Li, Yan-Lei Du","doi":"10.1093/jxb/eraf107","DOIUrl":"https://doi.org/10.1093/jxb/eraf107","url":null,"abstract":"Differences in phosphorus (P) utilisation efficiency (PUtE) and/or yield are closely linked to differences in root functional traits under low soil P availability. However, our understanding of how soil P availability mediates the intraspecific variation in root functional traits for breeding high-P efficiency genotypes to increase PUtE and yield remains limited. We investigated that plant growth parameters and pivotal root functional traits associated with P acquisition in 20 alfalfa genotypes with contrasting P efficiencies and supplied with low, medium or high levels of P. We observed that tradeoffs occurred in root functional traits among alfalfa genotypes under low-P stress. High-P efficiency genotypes displayed higher shoot biomass and PUtE by relying on thicker and more robust roots, elevated concentrations of carboxylate exudates and enhanced colonisation by arbuscular mycorrhizal fungi. In contrast, low-P efficiency genotypes exhibited a relatively high root-to-shoot ratio and primarily depended on higher tissue P concentrations but relatively slender roots along with comparatively high rhizosphere pH. Consequently, high PUtE and productivity under low-P conditions among alfalfa can be identified by screening for a phenotype with thick roots, increased exudate concentrations and mycorrhizal colonisation, opening up the potential for breeding for P-efficient lines in breeding programs.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metalloid transporters in plants: bridging the gap in molecular structure and physiological exaptation. 植物中的类金属转运体：弥合分子结构与生理适应之间的差距。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/erae261

Yogesh Sharma, Andrew M Hemmings, Rupesh Deshmukh, Ashwani Pareek

{"title":"Metalloid transporters in plants: bridging the gap in molecular structure and physiological exaptation.","authors":"Yogesh Sharma, Andrew M Hemmings, Rupesh Deshmukh, Ashwani Pareek","doi":"10.1093/jxb/erae261","DOIUrl":"10.1093/jxb/erae261","url":null,"abstract":"The rhizosphere contains both essential nutrients and potentially harmful substances for plant growth. Plants, as sessile organisms, must efficiently absorb the necessary nutrients while actively avoiding the uptake of toxic compounds. Metalloids, elements that exhibit properties of both metals and non-metals, can have different effects on plant growth, from being essential and beneficial to being toxic. This toxicity arises due to either the dosage of exposure or the specific elemental type. To utilize or detoxify these elements, plants have developed various transporters regulating their uptake and distribution in plants. Genomic sequence analysis suggests that such transporter families exist throughout the plant kingdom, from chlorophytes to higher plants. These transporters form defined families with related transport preferences. The isoforms within these families have evolved with specialized functions regulated by defined selectivity. Hence, understanding the chemistry of transporters to atomic detail is important to achieve the desired genetic modifications for crop improvement. We outline various adaptations in plant transport systems to deal with metalloids, including their uptake, distribution, detoxification, and homeostasis in plant tissues. Structural parallels are drawn to other nutrient transporter systems to support emerging themes of functional diversity of active sites of transporters, elucidating plant adaptations to utilize and extrude metalloid concentrations. Considering the observed physiological importance of metalloids, this review highlights the shared and disparate features in metalloid transport systems and their corresponding nutrient transporters.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1370-1389"},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving crop nutrient status: discovery, innovation, and translation. 改善作物营养状况：发现、创新和转化。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/eraf003

Robert D Hancock, Raul Huertas, Derek Stewart, Christine H Foyer

引用次数: 0

Heat stress in plants: sensing, signalling, and ferroptosis. 植物的热胁迫：感应、信号传递和铁变态。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/erae296

Ayelén Mariana Distéfano, Victoria Bauer, Milagros Cascallares, Gabriel Alejandro López, Diego Fernando Fiol, Eduardo Zabaleta, Gabriela Carolina Pagnussat

引用次数: 0

Grain lysine enrichment and improved stress tolerance in rice through protein engineering. 通过蛋白质工程富集谷粒赖氨酸并提高水稻的抗逆性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-13 DOI: 10.1093/jxb/erae414

Ray Singh Rathore, Manjari Mishra, Ashwani Pareek, Sneh Lata Singla-Pareek

{"title":"Grain lysine enrichment and improved stress tolerance in rice through protein engineering.","authors":"Ray Singh Rathore, Manjari Mishra, Ashwani Pareek, Sneh Lata Singla-Pareek","doi":"10.1093/jxb/erae414","DOIUrl":"10.1093/jxb/erae414","url":null,"abstract":"Amino acids are a major source of nourishment for people living in regions where rice is a staple food. However, rice grain is deficient in essential amino acids including lysine. The activity of the enzyme dihydrodipicolinate synthase (DHDPS) is crucial for lysine production in higher plants, but it is tightly regulated through feedback inhibition by its end product, lysine, leading to limited activity in the grain and resulting in low lysine accumulation. We identified lysine binding sites in the DHDPS enzyme and introduced key mutations to make DHDPS lysine feedback insensitive. Using in vivo analysis and functional complementation assays, we confirmed that protein engineering of the DHDPS renders it insensitive to lysine. Expression of mutated DHDPS resulted in 29% higher lysine and 15% higher protein accumulation in rice grains than in the wild type. Importantly, the lysine content in transgenic grains was maintained in cooked rice. The transgenic plants also exhibited enhanced stress tolerance along with higher antioxidant levels, improved photosynthesis, and higher grain yield compared to wild-type plants. We have shown that protein engineering of DHDPS in rice can lead to accumulation of lysine in grains and impart abiotic stress tolerance. This approach could improve health in regions with nutrient deficiencies and environmental stressors that challenge food production and human health.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"1408-1426"},"PeriodicalIF":5.6,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0