Journal of Experimental Botany最新文献_第5页

Long-day induced flowering requires DNA hypermethylation in orchardgrass. 长日照诱导开花需要果园草的 DNA 超甲基化。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-27 DOI: 10.1093/jxb/eraf015

Zhongfu Yang, Haidong Yan, Gang Nie, Jiajing Xiao, Jianping Wang, Guangyan Feng, Dandan Li, Linkai Huang, Xinquan Zhang

{"title":"Long-day induced flowering requires DNA hypermethylation in orchardgrass.","authors":"Zhongfu Yang, Haidong Yan, Gang Nie, Jiajing Xiao, Jianping Wang, Guangyan Feng, Dandan Li, Linkai Huang, Xinquan Zhang","doi":"10.1093/jxb/eraf015","DOIUrl":"https://doi.org/10.1093/jxb/eraf015","url":null,"abstract":"Flowering, a pivotal plant lifecycle event, is intricately regulated by environmental and endogenous signals via genetic and epigenetic mechanisms. Photoperiod is a crucial environmental cue that induces flowering by activating integrators through genetic and epigenetic pathways. However, the specific role of DNA methylation, a conserved epigenetic marker, in photoperiodic flowering remains unclear. This study integrated methylome, transcriptome, and gene expression analyses in orchardgrass (Dactylis glomerata) to elucidate the molecular mechanisms underlying long-day (LD) flowering. We found that LD treatment led to CHH hypermethylation, which was associated with the increased expression of RNA-dependent DNA methylation pathway components. LD-induced CHH hypermethylation in promoters correlated with upregulated photoperiod pathway genes and down-regulated miRNAs. The suppression of DNA methylation under LD conditions delays flowering, highlighting the critical role of hypermethylation. Additionally, the novel-miR1736-3p was identified as a negative regulator of DgFT. These findings elucidate the promotion of flowering through LD-induced CHH hypermethylation and provide insights into using epigenetic techniques to control plant flowering time.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential roles of mitochondrial carrier proteins in plant responses to abiotic stress.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-26 DOI: 10.1093/jxb/eraf032

Rita de Cássia Monteiro-Batista, João Antonio Siqueira, Paula da Fonseca Pereira, Pedro Barreto, Elias Feitosa-Araujo, Wagner L Araújo, Adriano Nunes-Nesi

引用次数: 0

Dancing Molecules: Group A bZIPs and PEBPs at the Heart of Plant Development and Stress Responses.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-26 DOI: 10.1093/jxb/eraf034

Damiano Martignago, Vítor da Silveira Falavigna, George Coupland, Lucio Conti

{"title":"Dancing Molecules: Group A bZIPs and PEBPs at the Heart of Plant Development and Stress Responses.","authors":"Damiano Martignago, Vítor da Silveira Falavigna, George Coupland, Lucio Conti","doi":"10.1093/jxb/eraf034","DOIUrl":"https://doi.org/10.1093/jxb/eraf034","url":null,"abstract":"Group A basic leucine zipper (bZIP) transcription factors play critical roles in abscisic acid (ABA) signaling and plant development. In Arabidopsis thaliana, these factors are defined by a highly conserved core bZIP domain, and four conserved domains throughout their length: three at the N-terminus (C1 to C3) and a phosphorylatable C-terminal SAP motif located at the C4 domain. Initially, members such as ABI5 and ABFs were studied for their roles in ABA signaling during seed germination or stress responses. Later, a sub-clade of group A bZIPs, including FD, was found to play important roles in floral induction by interacting with the florigen FLOWERING LOCUS T (FT) at the shoot apical meristem. Recent research has expanded our understanding of these transcription factors by identifying intriguing parallels between those involved in ABA signaling and those promoting floral induction, and revealing dynamic interactions with FT and other phosphatidylethanolamine-binding proteins (PEBP) such as TERMINAL FLOWER 1. Studies in crop plants and non-model species demonstrate broader roles, functions, and molecular targets of group A bZIPs. This review highlights common features of group A bZIPs and their post-translational regulation in enabling the activation of gene regulatory networks with important functions in plant development and stress responses.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Lipoxygenase Gene Modulates Jasmonate Biosynthesis to Enhance Blast Resistance in Rice.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-25 DOI: 10.1093/jxb/eraf026

Lanlan Wang, Yumeng Chen, Fan Deng, Gaochen Jin, Xue Gong, Gengwei Wu, Chi Zhang, Ran Li, Hua Wang

{"title":"A Lipoxygenase Gene Modulates Jasmonate Biosynthesis to Enhance Blast Resistance in Rice.","authors":"Lanlan Wang, Yumeng Chen, Fan Deng, Gaochen Jin, Xue Gong, Gengwei Wu, Chi Zhang, Ran Li, Hua Wang","doi":"10.1093/jxb/eraf026","DOIUrl":"https://doi.org/10.1093/jxb/eraf026","url":null,"abstract":"Inhibition of jasmonic acid (JA) signaling renders plants more susceptible to biotic stresses. Pathogen infection can induce an increase in JA levels. However, our understanding of the mechanisms mediating pathogen-induced JA accumulation in rice (Oryza sativa) remains limited. We characterized OsLOX9, a lipoxygenase gene of the 13-LOX subfamily involved in JA biosynthesis, which is induced upon infection by the rice blast fungus Magnaporthe oryzae. Two independent lox9 mutants exhibited increased susceptibility to M. oryzae infection, a phenotype that was fully restored by methyl-JA (MeJA) treatment, and showed a reduced pathogen-induced accumulation of 12-oxo-phytodienoic acid, JA, and its bioactive form JA-Ile. RNA sequencing-based expression analysis in response to M. oryzae infection revealed an essential role of OsLOX9-produced JAs in upregulating terpenoid phytoalexin pathway genes. Furthermore, we found that the JA core transcription factor, OsMYC2, can bind to the promoter of OsLOX9 and activate its expression, creating a positive feedback loop during pathogenesis. This study reveals that OsLOX9 is a key blast fungus-induced JA biosynthetic gene and contributes to rice resistance to blast disease.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis eATP receptor, DORN1 and CNGC19 channel act in tandem to regulate plant defense upon Spodoptera litura herbivory.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-25 DOI: 10.1093/jxb/eraf025

Pritha Kundu, Misha Kumari, Mukesh Kumar Meena, Shruti Mishra, Jyothilakshmi Vadassery

{"title":"The Arabidopsis eATP receptor, DORN1 and CNGC19 channel act in tandem to regulate plant defense upon Spodoptera litura herbivory.","authors":"Pritha Kundu, Misha Kumari, Mukesh Kumar Meena, Shruti Mishra, Jyothilakshmi Vadassery","doi":"10.1093/jxb/eraf025","DOIUrl":"https://doi.org/10.1093/jxb/eraf025","url":null,"abstract":"Plants deploy cellular Ca2+ elevation as a signal for environmental stress signaling. Extracellular ATP (eATP) is released into the extracellular matrix when cells are wounded. DOES NOT RESPOND TO NUCLEOTIDES 1 (DORN1), a key legume-type lectin receptor, senses and binds eATP and activates Ca2+ signaling. No evidence directly links calcium-mediated eATP signaling to resistance against insect herbivores. Here, we report upregulation of DORN1 transcripts upon wounding and Spodoptera litura feeding in Arabidopsis. Loss-of-function of DORN1 resulted in increased S. litura feeding compared to that on wildtype. Plant immunity is compromised in dorn1 mutants as they show reduced S. litura oral secretion mediated Ca2+ elevation, jasmonic acid accumulation, and expression of jasmonate responsive genes. The herbivory-induced calcium channel, CYCLIC NUCLEOTIDE GATED CHANNEL 19 (CNGC19), co-expresses with DORN1. We found that eATP-induced Ca2+ elevation requires functional CNGC19. Loss-of-function of DORN1 and CNGC19 highly increased the susceptibility to S. litura, mediated by reduced accumulation of jasmonates. We also demonstrate a plausible interaction of CNGC19 with DORN1. The data implicate the role of damage-released eATP and its receptor DORN1 in herbivory-induced defense signaling. DORN1 together with the Ca2+ channel CNGC19 generate the eATP-induced Ca2+ elevation, leading to the activation of immune signaling.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tool to measure maize root system stiffness that enables a comprehensive understanding of plant mechanics and lodging.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-24 DOI: 10.1093/jxb/erae465

Ashley N Hostetler, Jonathan W Reneau, Joseph Cristiano, Teclemariam Weldekidan, Taran A Kermani, Therese T Kim, Erin E Sparks

{"title":"A tool to measure maize root system stiffness that enables a comprehensive understanding of plant mechanics and lodging.","authors":"Ashley N Hostetler, Jonathan W Reneau, Joseph Cristiano, Teclemariam Weldekidan, Taran A Kermani, Therese T Kim, Erin E Sparks","doi":"10.1093/jxb/erae465","DOIUrl":"https://doi.org/10.1093/jxb/erae465","url":null,"abstract":"Plant mechanical failure, known as lodging, has detrimental impacts on the quality and quantity of maize yields. Failure can occur at stalks (stalk lodging) or at roots (root lodging). While previous research has focused on proxy measures for stalk stiffness, stalk strength, and root strength, there is a need to quantify the root system stiffness, which quantifies the force-displacement relationship. Here, we report a tool to quantify the root system stiffness of maize hybrids grown in different conditions. The results show that maize hybrids with a higher root system stiffness have a greater susceptibility to root lodging. This result is consistent with expected mechanical behavior, since higher root system stiffness values mean that the plant reaches the failure strength at lower displacements compared with a plant with lower root system stiffness. Collectively, this study describes the first tool to measure root system stiffness and enables a comprehensive understanding of the integrated plant mechanics and lodging.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143032927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation. 毛杨叶芽中二氢查尔酮特异性o -甲基转移酶与芽树脂形成有关。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-22 DOI: 10.1093/jxb/eraf020

Eerik-Mikael Piirtola, Dawei Ma, Jürgen Ehlting, C Peter Constabel

{"title":"A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation.","authors":"Eerik-Mikael Piirtola, Dawei Ma, Jürgen Ehlting, C Peter Constabel","doi":"10.1093/jxb/eraf020","DOIUrl":"https://doi.org/10.1093/jxb/eraf020","url":null,"abstract":"Production of secreted leaf bud resin is a mechanism for temperate trees to protect dormant leaf buds against frost damage, dehydration, and insect herbivory. Bud resins contain a wide variety of special metabolites including terpenoids, benzenoids, and phenolics. The leaf bud resins of Populus trichocarpa and P. balsamifera contain high concentrations of O-methylated dihydrochalcones, but the enzymes for methylating these compounds remain enigmatic. We used transcriptomics and differential gene expression analyses to identify a gene encoding a P. trichocarpa DHC-specific O-methyltransferase, which we named PtDOMT1. Detailed enzymatic analyses demonstrated PtDOMT1 to be a highly selective and regiospecific O-methyltransferase which methylates 4- and 4'-positions of dihydrochalcones using S-adenosyl-L-methionine as a methyl donor. PtDOMT1 did not methylate any other flavonoid or phenolic substrate tested. These findings establish the final step in the biosynthesis of O-methylated dihydrochalcones in poplar and represent the first molecular analysis of leaf bud resin biosynthesis in plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of alternative oxidase in the maintenance of cellular redox balance under hypoxia via participation in nitric oxide turnover. 通过参与一氧化氮的转换，替代氧化酶在缺氧条件下维持细胞氧化还原平衡中的作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-22 DOI: 10.1093/jxb/eraf021

Abir U Igamberdiev, Natalia V Bykova

{"title":"The role of alternative oxidase in the maintenance of cellular redox balance under hypoxia via participation in nitric oxide turnover.","authors":"Abir U Igamberdiev, Natalia V Bykova","doi":"10.1093/jxb/eraf021","DOIUrl":"https://doi.org/10.1093/jxb/eraf021","url":null,"abstract":"Alternative oxidase (AOX) regulates the level of reactive oxygen species and nitric oxide (NO) in plants. While under normoxic conditions it alleviates NO formation, there are several indications that in the conditions of low oxygen such as during seed germination before radicle protrusion, in meristematic stem cells, and in flooded roots AOX can be involved in the production of NO from nitrite. Whereas the first reports considered this role as indirect, more evidence is accumulated that AOX can act as a nitrite: NO reductase. Such activity of the structurally similar di-iron proteins in bacteria has been demonstrated. We review the literature on this topic and show that AOX can be induced under hypoxic conditions and participate in NO turnover via the phytoglobin-NO cycle. This results in the facilitation of glycolytic reactions by reoxidation of the glycolytically formed NADH and diverting the glycolytic carbon toward the formation of alanine and other amino acids. Pyruvate formed in glycolysis can activate AOX and facilitate its operation under these conditions. It is concluded that AOX is an important player in the hypoxic response in plants that regulates the redox level by participating in NO turnover as a nitrite: NO reductase in cooperation with nitrate reductase and phytoglobin.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FLOWERING LOCUS C-like mediates low-ambient-temperature-induced late flowering in chrysanthemum. 开花位点C-like介导菊花低环境温度诱导的晚花。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-21 DOI: 10.1093/jxb/eraf019

Qian Hu, Mengru Yin, Zheng Gao, Zixin Zhang, Yuqing Zhu, Rongqian Hu, Hua Cheng, Gaofeng Liu, Sumei Chen, Fadi Chen, Jiafu Jiang

{"title":"FLOWERING LOCUS C-like mediates low-ambient-temperature-induced late flowering in chrysanthemum.","authors":"Qian Hu, Mengru Yin, Zheng Gao, Zixin Zhang, Yuqing Zhu, Rongqian Hu, Hua Cheng, Gaofeng Liu, Sumei Chen, Fadi Chen, Jiafu Jiang","doi":"10.1093/jxb/eraf019","DOIUrl":"https://doi.org/10.1093/jxb/eraf019","url":null,"abstract":"The flowering time of Chrysanthemum morifolium predominantly depends on day length but is also sensitive to ambient temperature. However, the mechanisms underlying the response of chrysanthemum to ambient temperature are mainly unknown. This study identified a MADS-box transcription factor called CmFLC-like, a representative low ambient temperature-responsive factor induced in chrysanthemum leaves and shoot apical meristems at 15°C. Subsequently, CmFLC-like localizes to the cell nucleus and membrane and functions as a transcriptional repressor. CmFLC-like overexpression made plants more sensitive to low-temperature-induced late flowering, whereas the chimeric activator CmFLC-like-VP64 was less sensitive at 15°C, indicating that CmFLC-like was involved in thermosensory flowering. Transcriptome profiling of CmFLC-like transgenic plants suggested that the potential target genes for low ambient temperature-responsive CmFLC-like regulation are predominantly flowering integrators, MADS-box transcription factors, and AP2 genes. Subsequent examination revealed that the orchestrated repression of CmAFL1 and CmFTL3 by CmFLC-like was mediated by its direct binding to the CArG-box element of their promoters. This study offers novel insights into the molecular mechanisms underlying chrysanthemum flowering and highlights the essential role of CmFLC-like proteins in the thermosensory pathway.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesophyll conductance reductions due to drought stress are influenced by increases in cell wall chelator-soluble pectin content and denser microfibril alignment in cotton.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-01-21 DOI: 10.1093/jxb/erae467

Dongsheng Sun, Zhangying Lei, Marc Carriquí, Yujie Zhang, Tianyang Liu, Shengnan Wang, Kunhao Song, Lan Zhu, Wangfeng Zhang, Yali Zhang

{"title":"Mesophyll conductance reductions due to drought stress are influenced by increases in cell wall chelator-soluble pectin content and denser microfibril alignment in cotton.","authors":"Dongsheng Sun, Zhangying Lei, Marc Carriquí, Yujie Zhang, Tianyang Liu, Shengnan Wang, Kunhao Song, Lan Zhu, Wangfeng Zhang, Yali Zhang","doi":"10.1093/jxb/erae467","DOIUrl":"https://doi.org/10.1093/jxb/erae467","url":null,"abstract":"Plants commonly undergo leaf morphoanatomy and composition modifications to cope with drought stress, and these tend to reduce mesophyll conductance to CO2 diffusion (gm), a key limitation to photosynthesis. The cell wall appears to play a crucial role in this reduction, yet the specific effect of cell wall compositions on gm and the underlying regulatory mechanisms of cell wall thickness (Tcw) variation are not well understood. In this study, we subjected cotton plants to varying levels of water deficit to investigate the impact of leaf cell wall composition and the arrangement patterns of microfibrils within cell walls on Tcw and leaf gas exchange. Drought stress resulted in a significant thickening of cell walls and a decrease in gm. Concurrently, drought stress increased the content of chelator-soluble pectin and cellulose while reducing hemicellulose content. The alignment of cellulose microfibrils became more parallel and their diameter increased with under drought conditions, suggesting a decrease in cell wall effective porosity which coincides with the observed reduction in gm. This research demonstrates that reduced gm typically observed under drought stress conditions is related not only to thickened cell walls, but also to ultra-anatomical and compositional variations. Specifically, increases in cellulose content, diameter, and highly aligned arrangement collectively contributed to an increase in Tcw, which together with increases in chelator-soluble pectin content, resulted in an increased cell wall resistance to CO2 diffusion.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0