Journal of Experimental Botany最新文献_第5页

Beyond Bitter: Plant triterpenoids in the battle against herbivorous insects. 超越痛苦：植物三萜在对抗食草昆虫的战斗中。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-29 DOI: 10.1093/jxb/eraf238

Vandana Mathur, Narasimham Dokka, Gajalakshmi Raghunathan, Maniraj Rathinam, Manisha Parashar, Shraddha Srivastava, Rohini Sreevathsa

{"title":"Beyond Bitter: Plant triterpenoids in the battle against herbivorous insects.","authors":"Vandana Mathur, Narasimham Dokka, Gajalakshmi Raghunathan, Maniraj Rathinam, Manisha Parashar, Shraddha Srivastava, Rohini Sreevathsa","doi":"10.1093/jxb/eraf238","DOIUrl":"https://doi.org/10.1093/jxb/eraf238","url":null,"abstract":"Triterpenes are pivotal components of plant defence, offering a sustainable alternative to synthetic pesticides in insect pest management. They serve as critical components of both direct and indirect defence strategies, impacting various facets of herbivore behaviour and development. These structurally diverse metabolites not only deter herbivores directly but also modulate ecological interactions, potentially contributing to plant immunity. Despite their ecological and evolutionary significance, a comprehensive understanding of triterpene utility in plant defence, specifically towards herbivorous insects remains patchy. This review explores the evolutionary arms race between plants and insect herbivores, focusing on how pests evolved counter strategies against triterpene-based defences and the implications for long-term resistance management. However, their effective deployment requires a comprehensive framework that integrates evolutionary biology, ecological dynamics, and biotechnological interventions. Advancements in genome editing, metabolic engineering, and RNA interference (RNAi) provide promising avenues to optimize triterpene production in crops while targeting insect defence pathways. By integrating molecular, ecological, and biotechnological insights, this review proposes a conceptual framework to harness triterpenes for sustainable agriculture, reducing pesticide dependency while maintaining ecological balance. Addressing key knowledge gaps through interdisciplinary research will be critical for translating these discoveries into scalable agricultural solutions, ensuring crop resilience and long-term food security.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIPPs of all trades: Insights from pathogens on the function of HMA proteins at plasmodesmata. 各行各业的hipp：病原体对HMA蛋白在胞间连丝中的功能的见解。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-28 DOI: 10.1093/jxb/eraf234

Emma Kathleen Turley, Christine Faulkner

{"title":"HIPPs of all trades: Insights from pathogens on the function of HMA proteins at plasmodesmata.","authors":"Emma Kathleen Turley, Christine Faulkner","doi":"10.1093/jxb/eraf234","DOIUrl":"https://doi.org/10.1093/jxb/eraf234","url":null,"abstract":"Within plant cell walls, plasmodesmal channels harbour unique collections of proteins to maintain their structure and facilitate dynamic regulation of cell-to-cell connectivity. Proteomic surveys, combined with evidence from confocal microscopy, have identified heavy metal-associated (HMA) domain-containing proteins as residents at plasmodesmata; however, the functional relevance of this localisation is currently unknown. Although HMA domains themselves are present in all kingdoms of life, in plants they can be found in three main families: HPPs, HIPPs and P1B-type ATPases. Within the last decade, HPPs and HIPPs have emerged as frequent host targets of pathogen-derived molecules, including secreted effectors from bacteria, fungi and oomycetes, and a viral movement protein. The seemingly conserved targeting of HMA domains throughout distantly related pathosystems suggests these proteins could play integral roles in plant immunity. This is corroborated by observations of HMA-like domains being integrated into plant immune receptors, enabling direct binding of effectors to activate downstream signalling, as well as genetic evidence highlighting the influence of HPPs and HIPPs on disease susceptibility. Drawing especially from plant pathology studies, we speculate about the potential metallochaperone and signalling functions of these enigmatic plasmodesmal components.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Time to flowering and flowering duration in mungbean are unrelated physiological traits with independent genetic controls. 绿豆的花期和花期是不相关的生理性状，具有独立的遗传控制。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-28 DOI: 10.1093/jxb/eraf222

Caitlin Dudley, Shanice Van Haeften, Samir Alahmad, Eric Dinglasan, Lee T Hickey, Hannah Robinson, Christine Beveridge, Michael Udvardi, Thomas Noble, Karen Massel, Elizabeth A Dun, Millicent R Smith

{"title":"Time to flowering and flowering duration in mungbean are unrelated physiological traits with independent genetic controls.","authors":"Caitlin Dudley, Shanice Van Haeften, Samir Alahmad, Eric Dinglasan, Lee T Hickey, Hannah Robinson, Christine Beveridge, Michael Udvardi, Thomas Noble, Karen Massel, Elizabeth A Dun, Millicent R Smith","doi":"10.1093/jxb/eraf222","DOIUrl":"https://doi.org/10.1093/jxb/eraf222","url":null,"abstract":"Mungbean (Vigna radiata L.), a valuable sub-tropical grain legume, typically has a long, asynchronous flowering window, increasing vulnerability to abiotic stress and complicating harvest. To facilitate breeding, we extensively studied days to flowering (DTF) and the novel trait flowering duration (FD) in multi-environment trials. A diverse nested association mapping population was evaluated across four field experiments in Queensland, Australia (2022-2023). Extensive phenotypic variation was observed for DTF (35-70 days after sowing) and FD (20-60 days). Both traits displayed Genotype x Environment interactions, with FD showing stronger environmental interactions than DTF. No relationship was evident between DTF and FD across environments. Genome-wide association studies identified eight quantitative trait loci (QTL) for DTF and one for FD, with no overlapping QTL. The accumulation of early or late alleles at DTF QTL was associated with variations in flowering time. We have shown for the first time in mungbean that DTF and FD are independent traits with distinct genetic controls and environmental responses. This foundational understanding of mungbean flowering behaviour offers a mechanistic basis for developing varieties with optimized flowering patterns. Our findings provide crucial insights for future breeding programs, potentially enhancing mungbean adaptation and performance in diverse agricultural environments challenged by climate change.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clathrin Light Chain 2 is a Substrate of Ubiquitin Ligase ATL5 and Negatively Regulates Seed Longevity in Arabidopsis. 网格蛋白轻链2是泛素连接酶ATL5的底物，负调控拟南芥种子寿命。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-28 DOI: 10.1093/jxb/eraf231

Yuanyuan Lyu, Wenping He, Xinran Zhou, Xue Gao, Mingxia Fan, Defu Chen, Xiwen Chen

{"title":"Clathrin Light Chain 2 is a Substrate of Ubiquitin Ligase ATL5 and Negatively Regulates Seed Longevity in Arabidopsis.","authors":"Yuanyuan Lyu, Wenping He, Xinran Zhou, Xue Gao, Mingxia Fan, Defu Chen, Xiwen Chen","doi":"10.1093/jxb/eraf231","DOIUrl":"https://doi.org/10.1093/jxb/eraf231","url":null,"abstract":"Seed longevity is crucial for both ecological and agronomical value. Previously, we demonstrated that the E3 ligase Arabidopsis tóxicos en levadura 5 (ATL5) positively regulates seed longevity by mediating the degradation of the activator of basal transcription 1 in Arabidopsis. In the present study, we demonstrated that clathrin light chain 2 (CLC2), another ubiquitinated substrate of ATL5, affects seed longevity in Arabidopsis. The interaction between CLC2 and ATL5 was first identified in yeast cells and then in planta. Seeds of clc2 mutants displayed slower accelerated aging, whereas CLC2-OE seeds showed faster accelerated aging than wild-type seeds. In vitro assay showed that ATL5 promotes CLC2 degradation through the 26S proteasome pathway. Degradation of endogenous CLC2 was diminished in atl5 seeds, which could be induced by aging and occurs in a proteasome-dependent manner. Moreover, the role of CLC2 in seed longevity was independent of endocytosis; however, CLC2 exhibited transcriptional activation activity. Transcriptomic analysis revealed that the expression of numerous ribosomal protein genes was significantly upregulated in clc2 seeds after aging. Collectively, our study demonstrated that CLC2 is another ubiquitinated substrate of ATL5 that negatively regulates seed longevity by influencing the expression of ribosomal protein genes in Arabidopsis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arabidopsis homeodomain transcription factor BRASSINOSTEROID-RELATED HOMEOBOX 1 represses BR responses under stress conditions. 拟南芥同源结构域转录因子brassinosteroids相关的HOMEOBOX 1在胁迫条件下抑制BR反应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-28 DOI: 10.1093/jxb/eraf233

Reika Hasegawa, Miho Ikeda, Kenjiro Fujita, Yuichiro Tanaka, Hironori Takasaki, Yuka Kinugasa, Ryo Tachibana, Ayumi Yamagami, Takahito Nomura, Tsubasa Yano, Nobutaka Mitsuda, Takeshi Nakano, Masaru Ohme-Takagi

{"title":"Arabidopsis homeodomain transcription factor BRASSINOSTEROID-RELATED HOMEOBOX 1 represses BR responses under stress conditions.","authors":"Reika Hasegawa, Miho Ikeda, Kenjiro Fujita, Yuichiro Tanaka, Hironori Takasaki, Yuka Kinugasa, Ryo Tachibana, Ayumi Yamagami, Takahito Nomura, Tsubasa Yano, Nobutaka Mitsuda, Takeshi Nakano, Masaru Ohme-Takagi","doi":"10.1093/jxb/eraf233","DOIUrl":"https://doi.org/10.1093/jxb/eraf233","url":null,"abstract":"The brassinosteroid (BR) phytohormone is an important regulator of plant growth and stress responses. To identify novel transcription factors that regulate BR responses, we screened chimeric repressor gene silencing technology (CRES-T) plants, in which transcription factors were converted into chimeric repressors by the fusion of SRDX plant-specific repression domain, with brassinazole (Brz), an inhibitor of BR biosynthesis. We found a chimeric repressor of homeobox transcription factor, BRASSINOSTEROID-RELATED-HOMEOBOX-1 (BHB1-sx) induced a Brz-insensitive phenotype with longer hypocotyl, slender dwarf, and epinastic leaves and the expression of BR biosynthesis and inducible genes was enhanced in BHB1-sx. In contrast, ectopic expression of BHB1 (BHB1-ox) resulted in a Brz-hypersensitive phenotype with short hypocotyl, dwarf, and hyponastic leaves and the expression of BR associated genes was repressed in BHB1-ox. Transcriptome analysis revealed that the expression of auxin-related genes was downregulated, whereas that of stress-related genes were upregulated in BHB1-ox plants, indicating that BHB1 negatively regulates BR responses. The expression of BHB1 was induced by BR and stress condition, and BHB1-ox showed higher tolerance to salt stress, whereas bhb1 showed lower tolerance compared to the wild type. BHB1 may function as a hub factor mediating the crosstalk between salt stress and BR signaling.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene-based model to predict heading date in wheat based on allelic characterization and environmental drivers. 基于等位基因特征和环境驱动因素的小麦抽穗期基因预测模型。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf049

Mariana R Jardón, Santiago Alvarez-Prado, Leonardo Vanzetti, Fernanda G Gonzalez, Thomas Pérez-Gianmarco, Dionisio Gómez, Román A Serrago, Jorge Dubcovsky, Maria Elena Fernandez Long, Daniel J Miralles

{"title":"Gene-based model to predict heading date in wheat based on allelic characterization and environmental drivers.","authors":"Mariana R Jardón, Santiago Alvarez-Prado, Leonardo Vanzetti, Fernanda G Gonzalez, Thomas Pérez-Gianmarco, Dionisio Gómez, Román A Serrago, Jorge Dubcovsky, Maria Elena Fernandez Long, Daniel J Miralles","doi":"10.1093/jxb/eraf049","DOIUrl":"10.1093/jxb/eraf049","url":null,"abstract":"While numerous wheat phenology prediction models are available, most of them are constrained to using variety-dependent coefficients. The overarching objective of this study was to calibrate a gene-based model to predict wheat heading date that allows breeders to select specific gene combinations that would head within the optimal window for a given environment independently of varietal genetic background. A dataset with a total of 49 Argentine wheat cultivars and two recombinant inbred lines was chosen to cover a wide range of allelic combinations for major vernalization, photoperiod, and earliness per se genes. The model was validated using independent data from an Argentine wheat trial network that includes sites from a wide latitudinal range. Ultimately, using this gene-based model, simulations were made to identify optimal gene combinations (ideotypes) × site combinations in contrasting locations. The selected model accurately predicted heading date with an overall median error of 4.6 d. This gene-based crop model for wheat phenology allowed the identification of groups of gene combinations predicted to produce heads within a low-risk window and can be adapted to predict other phenological stages based on accessible climatic information and publicly available molecular markers, facilitating its adoption in wheat-growing regions worldwide.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2162-2176"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A survey of triploid block inconsistency: potential mechanisms and evolutionary implications on polyploid speciation. 三倍体阻滞不一致的研究：多倍体物种形成的潜在机制及其进化意义。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf050

Susnata Salony, Josselin Clo, Filip Kolář, Clément Lafon Placette

{"title":"A survey of triploid block inconsistency: potential mechanisms and evolutionary implications on polyploid speciation.","authors":"Susnata Salony, Josselin Clo, Filip Kolář, Clément Lafon Placette","doi":"10.1093/jxb/eraf050","DOIUrl":"10.1093/jxb/eraf050","url":null,"abstract":"The triploid block, primarily caused by endosperm developmental issues, is known as a significant barrier to interploidy hybridization among flowering plants and, thereby, polyploid speciation. However, its strength varies across taxa, with some instances of leakiness, questioning its universal role as a barrier. We conducted a literature survey to explore the causes of the variation in the strength of the triploid block across 11 angiosperm families. We assessed the impact of interploidy cross direction, types of endosperm development, endosperm persistence at seed maturity, and divergence between cytotypes using a Bayesian meta-analysis. We found a significant influence of the type of endosperm in shaping variation in triploid block strength. Other factors tested had no impact on triploid seed viability, probably due to limited data and inconsistencies in estimation methods across the literature. In addition, triploid seed viability in experimental crosses was sometimes correlated to the occurrence of triploid hybrids in nature, sometimes not, suggesting a mixed role for the triploid block in shaping interspecies gene flow. Altogether, our study highlights the need for unified approaches in future studies on the triploid block to advance our understanding of its variation and evolutionary implications.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2096-2111"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plastidial α-glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana. 拟南芥质体葡聚糖磷酸化酶调节麦芽糖糊精代谢并影响淀粉参数。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf041

Aakanksha Singh, Rajadurga Rajendran, Mark Aurel Schöttler, Xiaoping Li, Qingting Liu, Sidratul Nur Muntaha, Joerg Fettke

{"title":"The plastidial α-glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana.","authors":"Aakanksha Singh, Rajadurga Rajendran, Mark Aurel Schöttler, Xiaoping Li, Qingting Liu, Sidratul Nur Muntaha, Joerg Fettke","doi":"10.1093/jxb/eraf041","DOIUrl":"10.1093/jxb/eraf041","url":null,"abstract":"The plastidial α-glucan phosphorylase (PHS1) can catalyze the elongation and degradation of glucans, but its exact physiological role in plants is not completely deciphered. Many studies have indicated that PHS1 is involved in transitory starch turnover both in photosynthetic tissues and reserve starch accumulation in sink organs, by exerting its effects on the plastidial maltodextrin pools. Recent studies have also established its role in the mobilization of short maltooligosaccharides, thereby assisting in starch granule initiation. Here we report findings from studying four constitutive double knockout mutants related to plastidial maltodextrin metabolism, namely phs1dpe1, phs1ptst2, phs1pgm1, and phs1isa3 of Arabidopsis thaliana. We observed different effects on carbon partitioning in these double mutants. Carbon allocation between starch and sucrose in different double mutants varied with respect to time and light conditions, with significant overall changes in phs1dpe1. Furthermore, we uncovered a potential time-specific function of PHS1 in maltodextrin metabolism. Changes in maltodextrin turnover exerted effects on the starch granule number and size in the double mutants, especially phs1dpe1. We further assessed the double mutants in terms of photosynthetic efficiency and starch parameters, such as internal structure and morphology, in detail. We found that the different photosynthetic parameters in pgm1 and its corresponding double mutant were affected relative to the wild type and phs1. However, other double mutants were not impaired in terms of photosynthetic efficiency despite alterations in their maltooligosaccharide levels.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2222-2238"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Earthworms and arbuscular mycorrhizal fungi improve salt tolerance in maize through symplastic pathways. 蚯蚓和丛枝菌根真菌通过共塑途径提高玉米的耐盐性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf057

Binglei Wang, Mingxuan Xiao, Jia Cao, Chong Wang

引用次数: 0

Sweet control: intracellular sorting of glycoproteins in plants. 甜控制：植物中糖蛋白的细胞内分选。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf095

Wencai Qi, Bingqi Zhang, Jiakai Yue, Liang Zhang

引用次数: 0