Journal of Experimental Botany最新文献_第6页

A monomer-dimer switch modulates the activity of plant adenosine kinase.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf094

David Jaroslav Kopečný, Armelle Vigouroux, Jakub Bělíček, Martina Kopečná, Radka Končitíková, Jaroslava Friedecká, Václav Mik, Klára Supíková, Jan František Humplík, Marine Le Berre, Stephan Plancqueel, Miroslav Strnad, Klaus von Schwartzenberg, Ondřej Novák, Solange Moréra, David Kopečný

{"title":"A monomer-dimer switch modulates the activity of plant adenosine kinase.","authors":"David Jaroslav Kopečný, Armelle Vigouroux, Jakub Bělíček, Martina Kopečná, Radka Končitíková, Jaroslava Friedecká, Václav Mik, Klára Supíková, Jan František Humplík, Marine Le Berre, Stephan Plancqueel, Miroslav Strnad, Klaus von Schwartzenberg, Ondřej Novák, Solange Moréra, David Kopečný","doi":"10.1093/jxb/eraf094","DOIUrl":"https://doi.org/10.1093/jxb/eraf094","url":null,"abstract":"Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated the substrate preferences, oligomeric states and structures of ADKs from moss (Physcomitrella patens) and maize (Zea mays) alongside metabolomic and phenotypic analyses. We showed that dexamethasone-inducible ZmADK overexpressor lines in Arabidopsis can benefit from a higher number of lateral roots and larger root areas under nitrogen starvation. We discovered that maize and moss enzymes can form dimers upon increasing protein concentration, setting them apart from the monomeric human and protozoal ADKs. Structural and kinetic analyses revealed a catalytically inactive unique dimer. Within the dimer, both active sites are mutually blocked. The activity of moss ADKs, exhibiting a higher propensity to dimerize, was tenfold lower compared to maize ADKs. Two monomeric structures in a ternary complex highlight the characteristic transition from an open to a closed state upon substrate binding. This suggests that the oligomeric state switch can modulate the activity of moss ADKs and likely other plant ADKs. Moreover, dimer association represents a novel negative feedback mechanism, helping to maintain steady levels of adenosine and AMP.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced ureide partitioning improves soybean performance under drought stress.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf099

Sandi Win Thu, Mechthild Tegeder

{"title":"Enhanced ureide partitioning improves soybean performance under drought stress.","authors":"Sandi Win Thu, Mechthild Tegeder","doi":"10.1093/jxb/eraf099","DOIUrl":"https://doi.org/10.1093/jxb/eraf099","url":null,"abstract":"Soybean (Glycine max [L.] Merr.) fixes atmospheric nitrogen through a symbiotic relationship with rhizobia in root nodules to produce allantoin and allantoic acid. These ureides serve as primary nitrogen transport compounds moved from nodules to shoot in support of physiological functions and organ growth. Nodule ureide permease 1 (UPS1) is important for this transport process. Drought stress inhibits nitrogen fixation and reduces productivity in soybean, which has been associated with the accumulation of ureides in both nodule and shoot tissues. In this study, it was hypothesized that changes in ureide nodule-to-leaf-to-sink partitioning through manipulation of UPS1 function would alter ureide tissue levels, ultimately influencing soybean responses to drought stress. Soybean plants overexpressing UPS1 (UPS1-OE) were exposed to moderate and severe drought conditions. Changes in organ and phloem ureide levels indicated enhanced nodule-to-shoot ureide transport and increased sink nitrogen supply in the transgenic versus control wild-type plants. We further uncovered improvements in carbon fixation, partitioning and availability for nitrogen fixation, resulting in increased nitrogen gains and better growth of the drought-stressed UPS1-OE lines. Overall, our findings demonstrate that enhanced ureide partitioning not only contributes to improved soybean performance under well-watered conditions, but also under drought stress.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitrogen-driven changes in leaf anatomy affect the coupling between leaf mass per area and photosynthesis.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf108

Wenshi Hu, Shanshan Zhang, Wei Huang, Haibin Chang, Yinshui Li, Chiming Gu, Jing Dai, Lu Yang, Xing Liao, Lu Qin

{"title":"Nitrogen-driven changes in leaf anatomy affect the coupling between leaf mass per area and photosynthesis.","authors":"Wenshi Hu, Shanshan Zhang, Wei Huang, Haibin Chang, Yinshui Li, Chiming Gu, Jing Dai, Lu Yang, Xing Liao, Lu Qin","doi":"10.1093/jxb/eraf108","DOIUrl":"https://doi.org/10.1093/jxb/eraf108","url":null,"abstract":"Leaf mass per area (LMA) and photosynthetic rate (A) explain fast-slow growth strategies in worldwide leaf economic spectrum. Nitrogen (N) promotes A and rapid growth; while LMA response to N supply in a genotype specific manner. Structural traits affect the relationship between LMA and A, and we hypothesized that N supply would affect structural traits and thus the coupling between LMA and A. We tested this hypothesis by measuring A, LMA, anatomical traits and N allocation to various leaf components in 9 Brassica napus cultivars under two N supply levels. Mesophyll cell density (ρcell) and palisade tissue thickness (Tp) predominantly influence the variability in LMA. Enhanced Tp increased LMA, chloroplast surface area exposed to intercellular airspace, and N allocation into Rubisco (Nrub), thereby positively affecting mesophyll conductance (gm) and A. Conversely, ρcell promoted LMA but negatively affect Nrub under N deficiency. Enhanced LMA promoted N allocation to cell wall (Ncw), causing decreased Nrub fraction, and consequently A. This negative effect was relieved by the positive effect of Tp on A, which coupled the variation of LMA and A. The plasticity of Tp and ρcell regulating gm and trade-off between Nrub and Ncw provides insights for simultaneous increases in LMA and A to promote rapid growth and resistance.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-CG DNA methylation represses SDC expression to modulate hypocotyl elongation during thermormorphogenesis.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf105

Maián Garro, Eleonora Greco, Gustavo J Vannay, Aleksandra Leonova, Leonardo Bruno, Matías Capella

{"title":"Non-CG DNA methylation represses SDC expression to modulate hypocotyl elongation during thermormorphogenesis.","authors":"Maián Garro, Eleonora Greco, Gustavo J Vannay, Aleksandra Leonova, Leonardo Bruno, Matías Capella","doi":"10.1093/jxb/eraf105","DOIUrl":"https://doi.org/10.1093/jxb/eraf105","url":null,"abstract":"Plants adapt to warm environments through physiological and morphological changes termed thermomorphogenesis, which involve transcriptional reprogramming exerted mainly by PHYTOCHROME INTERACTING FACTOR 4 (PIF4). Fluctuating temperatures can also influence the patterns of cytosine DNA methylation, thereby influencing gene expression. However, whether these epigenetic changes provide an adaptative advantage remains unclear. Here, we provide evidence that DNA methylation is required to regulate thermomorphogenesis. Hypomethylated drm1 drm2 cmt3 mutants or seedlings treated with 5-azacytidine to block DNA methylation exhibit reduced hypocotyl growth at warm temperatures, primarily due to impaired cell elongation. Moreover, DNA hypomethylation compromises auxin biosynthesis and transport in response to warmth, partially by reducing PIF4 protein levels. Notably, the loss of DNA methylation leads to increased expression of SUPPRESSOR OF drm1 drm2 cmt3 (SDC), which in turn restricts hypocotyl elongation during thermomorphogenesis. Finally, we demonstrate that DNAme regulates the inhibition of SDC expression to promote gibberellin biosynthesis. Our findings underscore the critical role of DNA methylation in modulating gene expression in response to temperature fluctuations and provide new insights into the epigenetic regulation of thermomorphogenesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of silicon in crop protection against phloem feeding and chewing insect pests - a review. 硅在作物保护中抵御韧皮部取食和咀嚼害虫的潜力--综述。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf102

Monika Bathoova, Renata Svubova, Lukas Gimes, Dominik Kostolani, Ludmila Slovakova, Michal Martinka

{"title":"The potential of silicon in crop protection against phloem feeding and chewing insect pests - a review.","authors":"Monika Bathoova, Renata Svubova, Lukas Gimes, Dominik Kostolani, Ludmila Slovakova, Michal Martinka","doi":"10.1093/jxb/eraf102","DOIUrl":"https://doi.org/10.1093/jxb/eraf102","url":null,"abstract":"As future crop production is threatened by the increasing incidence of insect pests caused by changing climate, it is crucial to focus on searching for alternative methods of insect pest management. This review summarizes the current knowledge on the effect of silicon (Si) on the incidence, growth, development, longevity, survival, feeding efficiency, and reproduction parameters of phloem sap feeding and chewing insects of plants. While Si may negatively impact the biological parameters of both insect guilds, the effect is generally more pronounced in chewing insects, and often varies under different cultivation conditions. In addition to the direct effect of Si on insect pest performance, the potential mechanisms of Si-mediated resistance of infested plants are discussed. These involve increasing plant mechanical properties (Si-based or other organic barriers), and boosting biochemical defence responses, such as increased antioxidant activity, stimulated activity of defence enzymes, attraction of parasitoids and predators of insect pests, and synthesis of toxic and taste repelling compounds. These changes create a complex Si-induced defence system that enables infested plants to better withstand insect pest attack, ultimately reducing pest damage and improving agricultural crop yields.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grape Volatile Organic Compounds (VOCs)- Analysis, Biosynthesis and Profiling.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-08 DOI: 10.1093/jxb/eraf082

Iva Šikuten, Petra Štambuk, Zvjezdana Marković, Ivana Tomaz, Darko Preiner

{"title":"Grape Volatile Organic Compounds (VOCs)- Analysis, Biosynthesis and Profiling.","authors":"Iva Šikuten, Petra Štambuk, Zvjezdana Marković, Ivana Tomaz, Darko Preiner","doi":"10.1093/jxb/eraf082","DOIUrl":"10.1093/jxb/eraf082","url":null,"abstract":"Important contributors to grape quality are secondary metabolites, that will consequently define the wine quality and consumers acceptance. Secondary metabolites that influence the aromatic profile of grapes are volatile organic compounds (VOCs). Over the years several hundreds of VOCs have been identified in grapes and wines, ranging from trace amounts up to several milligrams, belonging to the classes of terpenoids (monoterpenes, sesquiterpenes, norisoprenoids), volatile phenols, methoxypyrazines, and aliphatic compounds. In grapes these compounds are influenced by numerous factors, such as grape variety, climate, and vineyard management practices. Thus, the analysis of grape VOCs, including the sample preparation, identification and quantification, is important to understand the influence of individual compounds in the aromatic profile. The biosynthetic pathways and genes involved are still not fully elucidated due to the large number of genes and precursors that participate in VOCs metabolism and contribute to the complexity. The aim of this paper is to provide an overview of volatile compounds synthesized in grape berries, explore their biosynthetic pathways, and discuss the analytical methods used for their identification and quantification.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically encoded biosensors as gateways to retrograde redox signalling in live plants.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-08 DOI: 10.1093/jxb/eraf080

José M Ugalde, Andreas J Meyer

{"title":"Genetically encoded biosensors as gateways to retrograde redox signalling in live plants.","authors":"José M Ugalde, Andreas J Meyer","doi":"10.1093/jxb/eraf080","DOIUrl":"10.1093/jxb/eraf080","url":null,"abstract":"In eukaryotic cells, protein supply to organelles varies depending on the stage of development and, in particular, on the exposure to environmental challenges. Adequate protein supply in terms of quality and quantity relies on sophisticated retrograde signalling systems that enable appropriate responses to the respective stress situations. Among many other retrograde signals, reactive oxygen species, that are being generated during the initial stress response, are thought to be involved in transduction of redox-related signals that may also involve multiple redox pairs such as NAD(P)H/NAD(P)+ and redox-active metabolites such as glutathione. Deciphering such signals requires detailed knowledge of their amplitudes and temporal and spatial dynamics. Genetically encoded biosensors based on fluorescent proteins have been developed for a number of different redox-related physiological parameters and can be monitored in living cells, tissues and even whole plants using a variety of instruments adapted to the respective resolution requirements, thus opening gateways to retrograde signalling in plant cells. This review summarizes and critically evaluates current probes and devices used to monitor sensor fluorescence. It also outlines how biosensors can be used in combination with genetic and pharmacological approaches, to extract meaningful information and dissect the retrograde redox signalling systems in living plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-08 DOI: 10.1093/jxb/eraf084

Claudio Osorio-Navarro, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, Lorena Norambuena

{"title":"The configuration of the vacuole is driven by clathrin-mediated trafficking in root cells of Arabidopsis thaliana.","authors":"Claudio Osorio-Navarro, Gabriel Neira-Valenzuela, Paula Sierra, Maciek Adamowski, Jorge Toledo, Lorena Norambuena","doi":"10.1093/jxb/eraf084","DOIUrl":"10.1093/jxb/eraf084","url":null,"abstract":"The central vacuole is a multifunctional organelle with the most significant occupancy in a differentiated plant cell. Plants depend on the function of the vacuole for critical development, growth, and environmental responses. As the cell expands, the vacuole changes shape and size, increasing its membrane and luminal content. The set of these events is called the vacuolar configuration process, which is scarcely described. Our research highlights the impact of plasma membrane internalization on vacuole morphology during the vacuolar configuration process. We observed a direct correlation between differential endocytosis rates and the enrichment of vacuolar membranous structures. Chemical and genetic interference of Clathrin-Mediated Endocytosis (CME) revealed that it is required for the vacuolar configuration of growing root cells. The contribution of CME to the vacuole configuration process co-occurs with the induction of post-TGN/EE trafficking with the participation of the Rab GTPases ARA6 and ARA7. Our results show that the CME plays an active role during the vacuole configuration, most likely carrying the material that allows the establishment of the vacuole on elongating cells. Since membrane trafficking through the EE/TGN is required to reach the vacuole, additional actors must be defined.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice. 更正：单一功能性抗稻瘟病基因 Pi54 激活了水稻复杂的防御机制

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-05 DOI: 10.1093/jxb/eraf054

引用次数: 0

Technological Advances on Imaging and Modelling of Leaf Structural Traits: A Review on Heat Stress in Wheat.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-04 DOI: 10.1093/jxb/eraf070

Jing He, Kun Ning, Afroz Naznin, Yuanyuan Wang, Chen Chen, Yuanyuan Zuo, Meixue Zhou, Chengdao Li, Rajeev Varshney, Zhong-Hua Chen

{"title":"Technological Advances on Imaging and Modelling of Leaf Structural Traits: A Review on Heat Stress in Wheat.","authors":"Jing He, Kun Ning, Afroz Naznin, Yuanyuan Wang, Chen Chen, Yuanyuan Zuo, Meixue Zhou, Chengdao Li, Rajeev Varshney, Zhong-Hua Chen","doi":"10.1093/jxb/eraf070","DOIUrl":"https://doi.org/10.1093/jxb/eraf070","url":null,"abstract":"Abiotic stresses such as heat waves significantly reduce wheat productivity by altering leaf anatomy and physiology, leading to reduced photosynthetic carbon assimilation and crop yield. Despite the advancement in various imaging technologies at the field, canopy, plant, tissue, cellular and subcellular levels, phenotyping of imaging-based leaf structural traits (e.g. vein density, stomatal density, stomatal aperture) for abiotic stresses is still time-consuming and expensive without the aid of artificial intelligence (AI) and machine learning (ML). This review consolidates current knowledge of wheat leaf structural and functional adaptations to heat stress and highlights key advancements in imaging technologies for studying these important phenotypic traits. Recent high-resolution, non-destructive imaging technologies, including confocal laser scanning microscopy, X-ray computed tomography, and optical coherence tomography, have enabled in vivo visualisation of plants. Integrating these imaging techniques with AI/ML facilitates high-throughput phenotyping and the modelling of stress responses. We emphasise the potential for future research to leverage these technological advancements in imaging and AI, combining imaging data with physiological and multi-omics studies to deepen the understanding of plant heat tolerance mechanisms. Such multidisciplinary integration in leaf structure phenotyping will accelerate the development of resilient wheat varieties, offering critical insights for crop improvement in the face of climate change.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0