Journal of Experimental Botany最新文献_第6页

Identification of a novel gene, Bryophyte Co-retained Gene 1, that has a positive role in desiccation tolerance in the moss Physcomitrium patens. 鉴定在青苔 Physcomitrium patens 的干燥耐受性中发挥积极作用的新基因 PpBCG1。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae332

Zexi Chen, Ping Li, Jianfang He, Wenbo Wang, Xiaojun Pu, Silin Chen, Bei Gao, Xuewen Wang, Rui-Liang Zhu, Wenya Yuan, Li Liu

{"title":"Identification of a novel gene, Bryophyte Co-retained Gene 1, that has a positive role in desiccation tolerance in the moss Physcomitrium patens.","authors":"Zexi Chen, Ping Li, Jianfang He, Wenbo Wang, Xiaojun Pu, Silin Chen, Bei Gao, Xuewen Wang, Rui-Liang Zhu, Wenya Yuan, Li Liu","doi":"10.1093/jxb/erae332","DOIUrl":"10.1093/jxb/erae332","url":null,"abstract":"The moss Physcomitrium patens is a model system for the evolutionary study of land plants, and as such, it may contain as yet unannotated genes with functions related to the adaptation to water deficiency that was required during the water-to-land transition. In this study, we identified a novel gene, Bryophyte Co-retained Gene 1 (BCG1), in P. patens that is responsive to dehydration and rehydration. Under de- and rehydration treatments, BCG1 was significantly co-expressed with DHNA, which encodes a dehydrin (DHN). Examination of previous microarray data revealed that BCG1 is highly expressed in spores, archegonia (female reproductive organ), and mature sporophytes. In addition, the bcg1 mutant showed reduced dehydration tolerance, and this was accompanied by a relatively low level of chlorophyll content during recovery. Comprehensive transcriptomics uncovered a detailed set of regulatory processes that were affected by the disruption to BCG1. Experimental evidence showed that BCG1 might function in antioxidant activity, the abscisic acid pathway, and in intracellular Ca2+ homeostasis to resist desiccation. Overall, our results provide insights into the role of a bryophyte co-retained gene in desiccation tolerance.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6609-6624"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment. 幼苗期角质层的发育及其转录组和代谢组之间的联系

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae311

Keting Chen, Rupam Kumar Bhunia, Matthew M Wendt, Grace Campidilli, Colton McNinch, Ahmed Hassan, Ling Li, Basil J Nikolau, Marna D Yandeau-Nelson

{"title":"Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.","authors":"Keting Chen, Rupam Kumar Bhunia, Matthew M Wendt, Grace Campidilli, Colton McNinch, Ahmed Hassan, Ling Li, Basil J Nikolau, Marna D Yandeau-Nelson","doi":"10.1093/jxb/erae311","DOIUrl":"10.1093/jxb/erae311","url":null,"abstract":"The plant cuticle is a complex extracellular lipid barrier that has multiple protective functions. This study investigated cuticle deposition by integrating metabolomics and transcriptomics data gathered from six different maize seedling organs of four genotypes, the inbred lines B73 and Mo17, and their reciprocal hybrids. These datasets captured the developmental transition of the seedling from heterotrophic skotomorphogenic growth to autotrophic photomorphogenic growth, a transition that is highly vulnerable to environmental stresses. Statistical interrogation of these data revealed that the predominant determinant of cuticle composition is seedling organ type, whereas the seedling genotype has a smaller effect on this phenotype. Gene-to-metabolite associations assessed by integrated statistical analyses identified three gene networks associated with the deposition of different elements of the cuticle: cuticular waxes; monomers of lipidized cell wall biopolymers, including cutin and suberin; and both of these elements. These gene networks reveal three metabolic programs that appear to support cuticle deposition, including processes of chloroplast biogenesis, lipid metabolism, and molecular regulation (e.g. transcription factors, post-translational regulators, and phytohormones). This study demonstrates the wider physiological metabolic context that can determine cuticle deposition and lays the groundwork for new targets for modulating the properties of this protective barrier.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6500-6522"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-regulated Arabidopsis GAT2 is a low affinity γ-aminobutyric acid transporter. 受胁迫调控的拟南芥 GAT2 是一种低亲和力的 -氨基丁酸（GABA）转运体。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae321

Stefan Meier, Robin Bautzmann, Nataliya Y Komarova, Viona Ernst, Marianne Suter Grotemeyer, Kirsten Schröder, Alexander C Haindrich, Adriana Vega Fernández, Christelle A M Robert, John M Ward, Doris Rentsch

{"title":"Stress-regulated Arabidopsis GAT2 is a low affinity γ-aminobutyric acid transporter.","authors":"Stefan Meier, Robin Bautzmann, Nataliya Y Komarova, Viona Ernst, Marianne Suter Grotemeyer, Kirsten Schröder, Alexander C Haindrich, Adriana Vega Fernández, Christelle A M Robert, John M Ward, Doris Rentsch","doi":"10.1093/jxb/erae321","DOIUrl":"10.1093/jxb/erae321","url":null,"abstract":"The four-carbon non-proteinogenic amino acid γ-aminobutyric acid (GABA) accumulates to high levels in plants in response to various abiotic and biotic stress stimuli, and plays a role in C:N balance, signaling, and as a transport regulator. Expression in Xenopus oocytes and voltage-clamping allowed the characterization of Arabidopsis GAT2 (At5g41800) as a low affinity GABA transporter with a K0.5GABA ~8 mM. l-Alanine and butylamine represented additional substrates. GABA-induced currents were strongly dependent on the membrane potential, reaching the highest affinity and highest transport rates at strongly negative membrane potentials. Mutation of Ser17, previously reported to be phosphorylated in planta, did not result in altered affinity. In a short-term stress experiment, AtGAT2 mRNA levels were up-regulated at low water potential and under osmotic stress (polyethylene glycol and mannitol). Furthermore, AtGAT2 promoter activity was detected in vascular tissues, maturating pollen, and the phloem unloading region of young seeds. Even though this suggested a role for AtGAT2 in long-distance transport and loading of sink organs, under the conditions tested neither AtGAT2-overexpressing plants, atgat2 or atgat1 T-DNA insertion lines, nor atgat1 atgat2 doubleknockout mutants differed from wild-type plants in growth on GABA, amino acid levels, or resistance to salt and osmotic stress.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6295-6311"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GABA transport: beyond stress? A closer look at AtGAT2. GABA 转运：超越压力？近距离观察 AtGAT2

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae390

Reuben Tayengwa

引用次数: 0

Nuclear localization of Arabidopsis HD-Zip IV transcription factor GLABRA2 is driven by importin α. 拟南芥 HD-Zip IV 转录因子 GLABRA 的核定位由导入素 α 驱动。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae326

Bilal Ahmad, Ruben Lerma-Reyes, Thiya Mukherjee, Hieu V Nguyen, Audra L Weber, Emily E Cummings, Waltraud X Schulze, Jeffrey R Comer, Kathrin Schrick

{"title":"Nuclear localization of Arabidopsis HD-Zip IV transcription factor GLABRA2 is driven by importin α.","authors":"Bilal Ahmad, Ruben Lerma-Reyes, Thiya Mukherjee, Hieu V Nguyen, Audra L Weber, Emily E Cummings, Waltraud X Schulze, Jeffrey R Comer, Kathrin Schrick","doi":"10.1093/jxb/erae326","DOIUrl":"10.1093/jxb/erae326","url":null,"abstract":"GLABRA2 (GL2), a class IV homeodomain leucine-zipper (HD-Zip IV) transcription factor from Arabidopsis, is a developmental regulator of specialized cell types in the epidermis. GL2 contains a monopartite nuclear localization sequence (NLS) that is conserved in most HD-Zip IV members across the plants. We demonstrate that NLS mutations affect nuclear transport and result in a loss-of-function phenotypes. NLS fusions to enhanced yellow fluorescent protein (EYFP) show that it is sufficient for nuclear localization in roots and trichomes. Despite partial overlap of the NLS with the homeodomain, genetic dissection indicates that nuclear localization and DNA binding are separable functions. Affinity purification of GL2 from plants followed by MS-based proteomics identified importin α (IMPα) isoforms as potential GL2 interactors. NLS structural prediction and molecular docking studies with IMPα-3 revealed major interacting residues. Cytosolic yeast two-hybrid assays and co-immunoprecipitation experiments with recombinant proteins verified NLS-dependent interactions between GL2 and several IMPα isoforms. IMPα triple mutants (impα-1,2,3) exhibit abnormal trichome formation and defects in GL2 nuclear localization in trichomes, consistent with tissue-specific and redundant functions of IMPα isoforms. Taken together, our findings provide mechanistic evidence for IMPα-dependent nuclear localization of GL2 in Arabidopsis, a process that is critical for cell type differentiation of the epidermis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6441-6461"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor EjNAC5 regulates loquat fruit chilling lignification. EjNAC5 的甲基化及其与其他转录因子的相互作用调控枇杷果实的冷木质化。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae333

Yiqing Huang, Zihao Liang, Jiao Lu, Mengxue Zhang, Xizhi Cao, Ruoqian Hu, Dongdong Li, Donald Grierson, Wenbo Chen, Changqing Zhu, Di Wu, Yanna Shi, Kunsong Chen

{"title":"The transcription factor EjNAC5 regulates loquat fruit chilling lignification.","authors":"Yiqing Huang, Zihao Liang, Jiao Lu, Mengxue Zhang, Xizhi Cao, Ruoqian Hu, Dongdong Li, Donald Grierson, Wenbo Chen, Changqing Zhu, Di Wu, Yanna Shi, Kunsong Chen","doi":"10.1093/jxb/erae333","DOIUrl":"10.1093/jxb/erae333","url":null,"abstract":"Changes in both lignin biosynthesis and DNA methylation have been reported to be associated with chilling stress in plants. When stored at low temperatures, red-fleshed loquat is prone to lignification, with increased lignin content and fruit firmness, which has deleterious effects on taste and eating quality. Here, we found that 5 °C storage mitigated the increasing firmness and lignin content of red-fleshed 'Dahongpao' ('DHP') loquat fruit that occurred during 0 °C storage. EjNAC5 was identified by integrating RNA sequencing with whole-genome bisulfite sequencing analysis of 'DHP' loquat fruit. The transcript levels of EjNAC5 were positively correlated with changes in firmness and negatively correlated with changes in DNA methylation level of a differentially methylated region in the EjNAC5 promoter. In white-fleshed 'Baisha' ('BS') loquat fruit, which do not undergo chilling-induced lignification at 0 °C, the transcripts of EjNAC5 remained low and the methylation level of the differentially methylated region in the EjNAC5 promoter was higher, compared with 'DHP' loquat fruit. Transient overexpression of EjNAC5 in loquat fruit and stable overexpression in Arabidopsis and liverwort led to an increase in lignin content. Furthermore, EjNAC5 interacts with EjERF39 and EjHB1 and activates the transcription of Ej4CL1 and EjPRX12 genes involved in lignin biosynthesis. This regulatory network involves different transcription factors from those involved in the lignification pathway. Our study indicates that EjNAC5 promoter methylation modulates EjNAC5 transcript levels and identifies novel EjNAC5-EjERF39-Ej4CL1 and EjNAC5-EjHB1-EjPRX12 regulatory modules involved in chilling induced-lignification.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6625-6643"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stomatal patterning is differently regulated in adaxial and abaxial epidermis in Arabidopsis. 拟南芥正面表皮和背面表皮的气孔形态调节方式不同。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae354

Pirko Jalakas, Ingmar Tulva, Nele Malvīne Bērziņa, Hanna Hõrak

{"title":"Stomatal patterning is differently regulated in adaxial and abaxial epidermis in Arabidopsis.","authors":"Pirko Jalakas, Ingmar Tulva, Nele Malvīne Bērziņa, Hanna Hõrak","doi":"10.1093/jxb/erae354","DOIUrl":"10.1093/jxb/erae354","url":null,"abstract":"Stomatal pores in leaves mediate CO2 uptake into the plant and water loss via transpiration. Most plants are hypostomatous with stomata present only in the lower leaf surface (abaxial epidermis). Many herbs, including the model plant Arabidopsis, have substantial numbers of stomata also on the upper (adaxial) leaf surface. Studies of stomatal development have mostly focused on abaxial stomata and very little is known of adaxial stomatal formation. We analysed the role of leaf number in determining stomatal density and stomatal ratio, and studied adaxial and abaxial stomatal patterns in Arabidopsis mutants deficient in known abaxial stomatal development regulators. We found that stomatal density in some genetic backgrounds varies between different fully expanded leaves, and thus we recommend using defined leaves for analyses of stomatal patterning. Our results indicate that stomatal development is at least partly independently regulated in adaxial and abaxial epidermis, as (i) plants deficient in ABA biosynthesis and perception have increased stomatal ratios, (ii) the epf1epf2, tmm, and sdd1 mutants have reduced stomatal ratios, (iii) erl2 mutants have increased adaxial but not abaxial stomatal index, and (iv) stomatal precursors preferentially occur in abaxial epidermis. Further studies of adaxial stomata can reveal new insights into stomatal form and function.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6476-6488"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11523041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbohydrate-active enzymes involved in rice cell wall metabolism. 参与水稻细胞壁代谢的碳水化合物活性酶。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae295

Tibo De Coninck, Tom Desmet, Els J M Van Damme

{"title":"Carbohydrate-active enzymes involved in rice cell wall metabolism.","authors":"Tibo De Coninck, Tom Desmet, Els J M Van Damme","doi":"10.1093/jxb/erae295","DOIUrl":"10.1093/jxb/erae295","url":null,"abstract":"Plant cell walls are complex, multifunctional structures, built up of polysaccharides and proteins. The configuration and abundance of cell wall constituents determine cellular elongation and plant growth. The emphasis of this review is on rice, a staple crop with economic importance, serving as model for grasses/cereals. Recent advancements have contributed to a better understanding of the grass/cereal cell wall. This review brings together current knowledge of the organization and metabolism of the rice cell wall, and addresses gaps in the information regarding the cell wall and enzymes involved. Several cell wall fractions, including cellulose, mixed-linkage glucans, and glucuronoarabinoxylans, are well understood in rice and other grasses/grains. Conversely, there are still open questions and missing links in relation to xyloglucans, glucomannans, pectin, lignin, and arabinogalactan proteins. There is still a large and untapped potential to identify carbohydrate-active enzymes (CAZymes), to characterize their activity, and to elucidate their involvement in the metabolism of the mentioned cell wall fractions. This review highlights the involvement of carbohydrate-active enzymes in rice cell wall metabolism, providing an update of current understanding with the aim of demarcating research areas with potential for further investigations.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6206-6227"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141563481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chrysanthemum lavandulifolium homolog CYCLIN A2;1 modulates cell division in ray florets. Chrysanthemum lavandulifolium homolog ClCYCA2;1 modulates cell division in ray florets.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae325

Peng Zhang, Yahui Wang, Zhimin Wang, Shengqiang Di, Xinyi Zhang, Di Ma, Zhilong Bao, Fangfang Ma

{"title":"Chrysanthemum lavandulifolium homolog CYCLIN A2;1 modulates cell division in ray florets.","authors":"Peng Zhang, Yahui Wang, Zhimin Wang, Shengqiang Di, Xinyi Zhang, Di Ma, Zhilong Bao, Fangfang Ma","doi":"10.1093/jxb/erae325","DOIUrl":"10.1093/jxb/erae325","url":null,"abstract":"The morphology of ray florets in chrysanthemums is tightly associated with cell division and expansion, both of which require proper progression of the cell cycle. Here, we identified a Chrysanthemum lavandulifolium homolog, CYCLIN A2;1 (CYCA2;1), the expression of which in ray florets is negatively correlated with petal width. We found that CYC2a, a TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factor in the CYCLOIDEA2 (CYC2) family, interacts with and stabilizes CYC2b, and the latter can bind to the promoter of CYCA2;1 to activate its transcription. Overexpression of CYCA2;1 in C. lavandulifolium reduced the size of capitula and ray florets. Cytological analysis revealed that CYCA2;1 overexpression inhibited both cell division and expansion via repression of the mitotic cell cycle in ray florets, the latitudinal development of which was more relatively negatively influenced, thereby leading to increased ratios of petal length to width at later developmental stages. Yeast two-hybrid library screening revealed multiple proteins that interacted with CYCA2;1 including ACTIN-RELATED PROTEIN7 (ARP7), and silencing ARP7 inhibited the development of ray florets. Co-immunoprecipitation assays confirmed that CYCA2;1 could induce the degradation of ARP7 to inhibit the development of ray florets. Taken together, our results indicate the presence of a regulatory network in ray floret development in chrysanthemum consisting of CYC2b-CYCA2;1-ARP7 that acts via governing mitosis. The identification of this network has the potential to facilitate breeding efforts targeted at producing novel ornamental traits in the flowers.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6423-6440"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of drought and frost responses in cool season grasses (Pooideae): was drought tolerance a precursor to frost tolerance? 冷季型禾本科植物对干旱和霜冻反应的进化：耐旱性是耐霜冻性的前身吗？

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-10-30 DOI: 10.1093/jxb/erae316

Sylvia Pal Stolsmo, Camilla Lorange Lindberg, Rebekka Eriksen Ween, Laura Schat, Jill Christine Preston, Aelys Muriel Humphreys, Siri Fjellheim

{"title":"Evolution of drought and frost responses in cool season grasses (Pooideae): was drought tolerance a precursor to frost tolerance?","authors":"Sylvia Pal Stolsmo, Camilla Lorange Lindberg, Rebekka Eriksen Ween, Laura Schat, Jill Christine Preston, Aelys Muriel Humphreys, Siri Fjellheim","doi":"10.1093/jxb/erae316","DOIUrl":"10.1093/jxb/erae316","url":null,"abstract":"Frost tolerance has evolved many times independently across flowering plants. However, conservation of several frost tolerance mechanisms among distant relatives suggests that apparently independent entries into freezing climates may have been facilitated by repeated modification of existing traits ('precursor traits'). One possible precursor trait for freezing tolerance is drought tolerance, because palaeoclimatic data suggest plants were exposed to drought before frost and several studies have demonstrated shared physiological and genetic responses to drought and frost stress. Here, we combine ecophysiological experiments and comparative analyses to test the hypothesis that drought tolerance acted as a precursor to frost tolerance in cool-season grasses (Pooideae). Contrary to our predictions, we measured the highest levels of frost tolerance in species with the lowest ancestral drought tolerance, indicating that the two stress responses evolved independently in different lineages. We further show that drought tolerance is more evolutionarily labile than frost tolerance. This could limit our ability to reconstruct the order in which drought and frost responses evolved relative to each other. Further research is needed to determine whether our results are unique to Pooideae or general for flowering plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"6405-6422"},"PeriodicalIF":5.6,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11522984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0