Journal of Experimental Botany最新文献_第6页

Grapevine adaptation to cold and heat stress. 葡萄藤对冷热胁迫的适应性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-03 DOI: 10.1093/jxb/eraf158

Yuchen Guo, Shenchang Li, Pedro García-Caparros, Lijun Wang, Zhenchang Liang

引用次数: 0

Proteostasis and Protein Quality Control in chloroplasts: mechanisms and novel insights related to protein mis-localization. 叶绿体中的蛋白质静止和蛋白质质量控制：与蛋白质错误定位相关的机制和新见解。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-02 DOI: 10.1093/jxb/eraf182

Nicolaj Jeran, Maxime Mercier, Paolo Pesaresi, Luca Tadini

{"title":"Proteostasis and Protein Quality Control in chloroplasts: mechanisms and novel insights related to protein mis-localization.","authors":"Nicolaj Jeran, Maxime Mercier, Paolo Pesaresi, Luca Tadini","doi":"10.1093/jxb/eraf182","DOIUrl":"https://doi.org/10.1093/jxb/eraf182","url":null,"abstract":"The dynamic rearrangement of the proteome and the maintenance of protein homeostasis (proteostasis) are crucial for the proper development and functionality of cellular compartments. Disruptions in proteostasis can severely compromise cellular health, leading to the accumulation of misfolded or mis-localized proteins prone to forming toxic aggregates. In chloroplasts, proteostasis presents unique challenges due to their endosymbiotic origin, complex sub-compartmentalization, and constant exposure to reactive oxygen species (ROS) generated during photosynthesis. To counteract these challenges, chloroplasts employ sophisticated quality control systems, including chaperones, proteases, and protein degradation pathways such as ubiquitination and autophagy-related mechanisms. Additionally, cytosolic systems play a crucial role in regulating nuclear-encoded, plastid-targeted proteins, ensuring their proper delivery or degradation when defective. Within chloroplasts, specialized proteases, chaperones, and the chloroplast unfolded protein response (cpUPR) oversee protein quality and resolve aggregates to maintain functional integrity. This review critically examines mechanisms governing intracellular trafficking of plastid-targeted proteins, emphasizing key pathways and regulatory bottlenecks that, when disrupted, lead to the accumulation of mis-localized or orphaned proteins. Particular focus is given to the signalling pathways that coordinate cytosolic and plastid effectors to sustain chloroplast function. Furthermore, we propose a novel role for PSBO, subunit of the Oxygen Evolving Complex associated with the Photosystem II, in linking proplastid-to-chloroplast differentiation with plastid quality control.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Signaling peptides control beneficial and pathogenic plant-microbe interactions. 信号肽控制有益和致病植物与微生物的相互作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-02 DOI: 10.1093/jxb/eraf180

Yerisf C Torres Ascurra, Lena Maria Müller

{"title":"Signaling peptides control beneficial and pathogenic plant-microbe interactions.","authors":"Yerisf C Torres Ascurra, Lena Maria Müller","doi":"10.1093/jxb/eraf180","DOIUrl":"https://doi.org/10.1093/jxb/eraf180","url":null,"abstract":"Interactions between organisms, such as those between plants and microbes, require extensive signaling between and within each organism to detect and recognize the partner and elicit an appropriate response. Multiple families of small signaling peptides regulate plant interactions with beneficial or pathogenic microbes, and sometimes both. Some of these signaling peptides transmit information between different cells or organs of the host and allow plants to orchestrate a coordinated response towards microbial mutualists or pathogens. However, not only plants produce signaling peptides required for the interactions. Microbes themselves also secrete peptide signals, which are detected by host receptors and required for infection. Among these are microbial peptides mimicking those of plants, allowing mutualistic or pathogenic microbes to hijack endogenous plant signaling pathways and evade the host immune system. In this review, we provide a comprehensive summary of current knowledge on host- and microbe-derived signaling peptides and their cognate receptors regulating mutualistic and parasitic plant-microbe interactions. Furthermore, we describe how microbes hijack endogenous host signaling pathways, and discuss possible crosstalk between the plant signaling pathways controlling mutualism with those modulating immune responses to pathogens.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction of: Melatonin synthesis enzymes interact with ascorbate peroxidase to protect against oxidative stress in cassava. 褪黑素合成酶与抗坏血酸过氧化物酶相互作用，保护木薯免受氧化应激。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-02 DOI: 10.1093/jxb/eraf078

引用次数: 0

"Oxygen and derived reactive species in Legume - Rhizobia interactions: paradoxes and dual roles". 豆科植物-根瘤菌相互作用中的氧和衍生活性物质：悖论和双重作用。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-02 DOI: 10.1093/jxb/eraf160

Eliane Meilhoc, Alexandre Boscari, Nicolas Pauly, Marc Lepetit, Pierre Frendo, Claude Bruand, Alain Puppo, Renaud Brouquisse

{"title":"\"Oxygen and derived reactive species in Legume - Rhizobia interactions: paradoxes and dual roles\".","authors":"Eliane Meilhoc, Alexandre Boscari, Nicolas Pauly, Marc Lepetit, Pierre Frendo, Claude Bruand, Alain Puppo, Renaud Brouquisse","doi":"10.1093/jxb/eraf160","DOIUrl":"https://doi.org/10.1093/jxb/eraf160","url":null,"abstract":"Biological nitrogen fixation (BNF) between legumes and Rhizobia is the result of a symbiosis characterized by the formation of a new organ, the nodule, in which the plants house and feed the bacteria. Oxygen (O2) concentration inside the nodule is very low (on the order of a few tens of nanomolar). The nitrogenase which is responsible for the reduction of atmospheric nitrogen (N2) to ammonia (NH3) is irreversibly inhibited by traces of O2, while O2 is required for the overall process of N2 fixation which consumes high amounts of energy and reducing power. How is this paradox solved? The set-up of a physical and chemical O2 diffusion barrier, including the synthesis of numerous leghemoglobins, a class of hemoproteins with a very high O2 absorption capacity, was evidenced. However, why are so many leghemoglobin genes present while they appear to be mainly expressed in the same nodule zone? Furthermore, why do the bacterial symbionts contain multiple O2 sensors despite the existence of a very low O2 tension inside the nodule? On the other hand, the O2 derived reactive species, such as superoxide radical, hydrogen peroxide and nitric oxide, which play important metabolic and signalling roles in the symbiotic process, appear to act as Janus molecules. They exhibit opposite effects throughout symbiosis establishment and nodule life. The aim of this review is to provide possible answers to the questions asked and to highlight the dual roles of O2 reactive species in nodule development, functioning and senescence.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Superior haplotypes of genes associated with higher grain yield under reproductive stage drought stress in rice. 水稻生殖期干旱胁迫下高产基因的优势单倍型。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-28 DOI: 10.1093/jxb/eraf150

Preeti Singh, Bandana Pahi, Krishna T Sundaram, Niranjani Gnanapragasam, Sonali Vijay Habde, Nandigam Vinay Kumar, Namrata Devidas Patil, Pronob J Paul, Ravi Shekhar Kumar, Suresh Prasad Singh, Sanjay Kalia, Arvind Kumar, Ajay Kohli, Uma Maheshwar Singh, Vikas Kumar Singh, Pallavi Sinha

{"title":"Superior haplotypes of genes associated with higher grain yield under reproductive stage drought stress in rice.","authors":"Preeti Singh, Bandana Pahi, Krishna T Sundaram, Niranjani Gnanapragasam, Sonali Vijay Habde, Nandigam Vinay Kumar, Namrata Devidas Patil, Pronob J Paul, Ravi Shekhar Kumar, Suresh Prasad Singh, Sanjay Kalia, Arvind Kumar, Ajay Kohli, Uma Maheshwar Singh, Vikas Kumar Singh, Pallavi Sinha","doi":"10.1093/jxb/eraf150","DOIUrl":"https://doi.org/10.1093/jxb/eraf150","url":null,"abstract":"Understanding the genetic basis of reproductive stage drought tolerance (RSDT) requires bridging genomic variations with phenotype. This study employed a genome-wide association study (GWAS) using 450 rice accessions for grain yield under reproductive stage drought stress across three locations and two seasons. Results revealed 67 marker-trait associations (MTAs) that corresponded to 23 candidate genes. Superior haplotypes identified for ten RSDT-related genes including SAP-H22, WRKY109-H6, OsIRO2-H3, OsSOBIR-H68, OsPGL31-H8, OsDAHPS1-H2, ZCD-H13, OsMC2-H8, Ospdr9-H16, and SDR-H2.These genes are involved in stress related processes such as transcription regulation, cell wall structure modification, leaf rolling, programmed cell death, redox stress and secondary metabolite accumulation. Notably, introgressing superior haplotypes from the OsIRO2-H3 gene into elite rice cultivar led to yield advantage of 25.0 % to 27.3 % over recurrent parents. These findings highlight the potential of these genes as valuable resources for breeding high-yielding, drought-tolerant rice varieties.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Climate-smart trees: CLE peptides shaping future forests. 气候智能型树木：CLE肽塑造未来森林。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-26 DOI: 10.1093/jxb/eraf093

Madhab Kumar Sen, Rohit Bharati

引用次数: 0

Mixing varieties mitigates early root competition in wheat under water and nutrient limitation. 混合品种减轻了水分和养分限制下小麦早期根系竞争。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-26 DOI: 10.1093/jxb/eraf163

Germain Montazeaud, Pierre Roumet, Mickaël Lamboeuf, Christian Jeudy, Martin Ecarnot, Lise Malicet-Chebbah, Christophe Salon, Hélène Fréville

{"title":"Mixing varieties mitigates early root competition in wheat under water and nutrient limitation.","authors":"Germain Montazeaud, Pierre Roumet, Mickaël Lamboeuf, Christian Jeudy, Martin Ecarnot, Lise Malicet-Chebbah, Christophe Salon, Hélène Fréville","doi":"10.1093/jxb/eraf163","DOIUrl":"https://doi.org/10.1093/jxb/eraf163","url":null,"abstract":"Competition between plants can lead to a Tragedy of the Commons (TOCs) where over-investments into resource harvesting organs reduce collective performance. Mixing different crop varieties could resolve such TOCs and thus maintain high productivity while increasing within field diversity because varieties might have different resource requirements (positive niche complementarity effect) or because the most competitive varieties benefit from being mixed with weaker competitors (positive selection effect). To date, most studies on varietal mixtures have focused on the aboveground compartment while belowground competition remains poorly understood due to the challenges associated with accessing root traits. In the present study, we grew durum wheat (Triticum turgidum ssp. durum) varietal mixtures in a high-throughput root phenotyping platform to characterize early-stage belowground competition between varieties. We grew 36 varieties in pure stands and in 54 binary mixtures under both unlimited resource conditions (R+) and water and nutrient limitation (R-). Seedlings in mixed stands produced less biomass than predicted from their pure stands in R-, which mainly resulted from a negative complementarity effect. Around 50% of this biomass reduction was explained by the average projected area of the root systems of the two varieties in pure stands, and instead of a negative interaction between varieties, this negative complementarity effect reflected a relaxation of competition in mixed stands: varieties with high root projected area were strong competitors that over-invested in belowground organs in pure stands. They benefited from having, on average, a weaker competitor than themselves in mixed stands and thus disengaged from the arms race for biomass accumulation. Our results suggest that root area is a promising breeding targets to reduce intra-specific competition and a key trait to consider for mixture assembly.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein Acetylation as a Novel Post-translational Modification of Plant Peroxisomal Proteins. 蛋白质乙酰化是植物过氧化物酶体蛋白翻译后修饰的一种新方法。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-26 DOI: 10.1093/jxb/eraf089

Sigrun Reumann, Athina Parasyri

{"title":"Protein Acetylation as a Novel Post-translational Modification of Plant Peroxisomal Proteins.","authors":"Sigrun Reumann, Athina Parasyri","doi":"10.1093/jxb/eraf089","DOIUrl":"https://doi.org/10.1093/jxb/eraf089","url":null,"abstract":"Plant peroxisomes compartmentalize many important metabolic functions, but little is known how these pathways are regulated at the post-translational level. Few plant peroxisomal proteins have been shown to be subjected to reversible phosphorylation or ubiquitination, but other post-translational modifications are hardly known for peroxisomes from animals, fungi, and plants. We here address the question whether plant peroxisomal metabolism might be regulated by protein acetylation. We summarize available knowledge on protein acetylation in plastids and mitochondria, focusing on the catalytic machinery and the regulation of target enzymes. We screened global acetylome studies of Arabidopsis for known proteins of peroxisomes that are N-terminally or Lys acetylated. For selected matrix proteins, we mapped the acetylated Lys sites onto their AlphaFold 3D models to predict their effect on enzyme activity and oligomerization. We also summarize knowledge on two Arabidopsis acetyl transferases that have recently been identified as novel peroxisomal matrix proteins. We deduce their evolution in peroxisomes and partly their functions, as far as they can be predicted from available structural models. This information allows us to identify experimental strategies to define the postulated new regulatory mechanism of protein acetylation for plant peroxisomes in the near future. (193 words, <200).","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating plant morphogenesis using 3D digital organs. 利用三维数字器官研究植物形态发生。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-04-26 DOI: 10.1093/jxb/eraf091

Tejasvinee A Mody, Ratula Ray, Kay Schneitz

{"title":"Investigating plant morphogenesis using 3D digital organs.","authors":"Tejasvinee A Mody, Ratula Ray, Kay Schneitz","doi":"10.1093/jxb/eraf091","DOIUrl":"https://doi.org/10.1093/jxb/eraf091","url":null,"abstract":"How plant organs acquire their three-dimensional size and shape remains a prominent question in plant biology. Central questions revolve around how differential gene activity influences the growth of individual cell assemblies and how such growth patterns affect organ form. However, our understanding of morphogenetic processes at the cellular and supracellular level remains scarce because the necessary analyses have been notoriously difficult to perform with classical methods, especially in three dimensions and in deeper tissues. In recent years, significant improvements in imaging, artificial intelligence-based image processing, and 3D cell segmentation have enabled the generation of 3D digital plant organs with single-cell resolution. This review first describes the experimental toolbox that enables the generation and analysis of 3D digital organs. It then highlights a number of studies that illustrate their value for understanding plant morphogenesis. Finally, the review explores how cross-species comparative analysis of 3D digital organs can reveal evolutionary shifts in cellular patterns and their contribution to the astonishing diversity of morphology observed throughout the plant kingdom. Overall, the review aims to demonstrate how the advent of 3D digital organs has significantly broadened the range of approaches and opened new frontiers for the study of the cellular basis of tissue morphogenesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0