Journal of Experimental Botany最新文献_第6页

Arabidopsis homeodomain transcription factor BRASSINOSTEROID-RELATED HOMEOBOX 1 represses BR responses under stress conditions. 拟南芥同源结构域转录因子brassinosteroids相关的HOMEOBOX 1在胁迫条件下抑制BR反应。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-28 DOI: 10.1093/jxb/eraf233

Reika Hasegawa, Miho Ikeda, Kenjiro Fujita, Yuichiro Tanaka, Hironori Takasaki, Yuka Kinugasa, Ryo Tachibana, Ayumi Yamagami, Takahito Nomura, Tsubasa Yano, Nobutaka Mitsuda, Takeshi Nakano, Masaru Ohme-Takagi

{"title":"Arabidopsis homeodomain transcription factor BRASSINOSTEROID-RELATED HOMEOBOX 1 represses BR responses under stress conditions.","authors":"Reika Hasegawa, Miho Ikeda, Kenjiro Fujita, Yuichiro Tanaka, Hironori Takasaki, Yuka Kinugasa, Ryo Tachibana, Ayumi Yamagami, Takahito Nomura, Tsubasa Yano, Nobutaka Mitsuda, Takeshi Nakano, Masaru Ohme-Takagi","doi":"10.1093/jxb/eraf233","DOIUrl":"https://doi.org/10.1093/jxb/eraf233","url":null,"abstract":"The brassinosteroid (BR) phytohormone is an important regulator of plant growth and stress responses. To identify novel transcription factors that regulate BR responses, we screened chimeric repressor gene silencing technology (CRES-T) plants, in which transcription factors were converted into chimeric repressors by the fusion of SRDX plant-specific repression domain, with brassinazole (Brz), an inhibitor of BR biosynthesis. We found a chimeric repressor of homeobox transcription factor, BRASSINOSTEROID-RELATED-HOMEOBOX-1 (BHB1-sx) induced a Brz-insensitive phenotype with longer hypocotyl, slender dwarf, and epinastic leaves and the expression of BR biosynthesis and inducible genes was enhanced in BHB1-sx. In contrast, ectopic expression of BHB1 (BHB1-ox) resulted in a Brz-hypersensitive phenotype with short hypocotyl, dwarf, and hyponastic leaves and the expression of BR associated genes was repressed in BHB1-ox. Transcriptome analysis revealed that the expression of auxin-related genes was downregulated, whereas that of stress-related genes were upregulated in BHB1-ox plants, indicating that BHB1 negatively regulates BR responses. The expression of BHB1 was induced by BR and stress condition, and BHB1-ox showed higher tolerance to salt stress, whereas bhb1 showed lower tolerance compared to the wild type. BHB1 may function as a hub factor mediating the crosstalk between salt stress and BR signaling.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144159021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene-based model to predict heading date in wheat based on allelic characterization and environmental drivers. 基于等位基因特征和环境驱动因素的小麦抽穗期基因预测模型。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf049

Mariana R Jardón, Santiago Alvarez-Prado, Leonardo Vanzetti, Fernanda G Gonzalez, Thomas Pérez-Gianmarco, Dionisio Gómez, Román A Serrago, Jorge Dubcovsky, Maria Elena Fernandez Long, Daniel J Miralles

{"title":"Gene-based model to predict heading date in wheat based on allelic characterization and environmental drivers.","authors":"Mariana R Jardón, Santiago Alvarez-Prado, Leonardo Vanzetti, Fernanda G Gonzalez, Thomas Pérez-Gianmarco, Dionisio Gómez, Román A Serrago, Jorge Dubcovsky, Maria Elena Fernandez Long, Daniel J Miralles","doi":"10.1093/jxb/eraf049","DOIUrl":"10.1093/jxb/eraf049","url":null,"abstract":"While numerous wheat phenology prediction models are available, most of them are constrained to using variety-dependent coefficients. The overarching objective of this study was to calibrate a gene-based model to predict wheat heading date that allows breeders to select specific gene combinations that would head within the optimal window for a given environment independently of varietal genetic background. A dataset with a total of 49 Argentine wheat cultivars and two recombinant inbred lines was chosen to cover a wide range of allelic combinations for major vernalization, photoperiod, and earliness per se genes. The model was validated using independent data from an Argentine wheat trial network that includes sites from a wide latitudinal range. Ultimately, using this gene-based model, simulations were made to identify optimal gene combinations (ideotypes) × site combinations in contrasting locations. The selected model accurately predicted heading date with an overall median error of 4.6 d. This gene-based crop model for wheat phenology allowed the identification of groups of gene combinations predicted to produce heads within a low-risk window and can be adapted to predict other phenological stages based on accessible climatic information and publicly available molecular markers, facilitating its adoption in wheat-growing regions worldwide.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2162-2176"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A survey of triploid block inconsistency: potential mechanisms and evolutionary implications on polyploid speciation. 三倍体阻滞不一致的研究：多倍体物种形成的潜在机制及其进化意义。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf050

Susnata Salony, Josselin Clo, Filip Kolář, Clément Lafon Placette

{"title":"A survey of triploid block inconsistency: potential mechanisms and evolutionary implications on polyploid speciation.","authors":"Susnata Salony, Josselin Clo, Filip Kolář, Clément Lafon Placette","doi":"10.1093/jxb/eraf050","DOIUrl":"10.1093/jxb/eraf050","url":null,"abstract":"The triploid block, primarily caused by endosperm developmental issues, is known as a significant barrier to interploidy hybridization among flowering plants and, thereby, polyploid speciation. However, its strength varies across taxa, with some instances of leakiness, questioning its universal role as a barrier. We conducted a literature survey to explore the causes of the variation in the strength of the triploid block across 11 angiosperm families. We assessed the impact of interploidy cross direction, types of endosperm development, endosperm persistence at seed maturity, and divergence between cytotypes using a Bayesian meta-analysis. We found a significant influence of the type of endosperm in shaping variation in triploid block strength. Other factors tested had no impact on triploid seed viability, probably due to limited data and inconsistencies in estimation methods across the literature. In addition, triploid seed viability in experimental crosses was sometimes correlated to the occurrence of triploid hybrids in nature, sometimes not, suggesting a mixed role for the triploid block in shaping interspecies gene flow. Altogether, our study highlights the need for unified approaches in future studies on the triploid block to advance our understanding of its variation and evolutionary implications.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2096-2111"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plastidial α-glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana. 拟南芥质体葡聚糖磷酸化酶调节麦芽糖糊精代谢并影响淀粉参数。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf041

Aakanksha Singh, Rajadurga Rajendran, Mark Aurel Schöttler, Xiaoping Li, Qingting Liu, Sidratul Nur Muntaha, Joerg Fettke

{"title":"The plastidial α-glucan phosphorylase modulates maltodextrin metabolism and affects starch parameters in Arabidopsis thaliana.","authors":"Aakanksha Singh, Rajadurga Rajendran, Mark Aurel Schöttler, Xiaoping Li, Qingting Liu, Sidratul Nur Muntaha, Joerg Fettke","doi":"10.1093/jxb/eraf041","DOIUrl":"10.1093/jxb/eraf041","url":null,"abstract":"The plastidial α-glucan phosphorylase (PHS1) can catalyze the elongation and degradation of glucans, but its exact physiological role in plants is not completely deciphered. Many studies have indicated that PHS1 is involved in transitory starch turnover both in photosynthetic tissues and reserve starch accumulation in sink organs, by exerting its effects on the plastidial maltodextrin pools. Recent studies have also established its role in the mobilization of short maltooligosaccharides, thereby assisting in starch granule initiation. Here we report findings from studying four constitutive double knockout mutants related to plastidial maltodextrin metabolism, namely phs1dpe1, phs1ptst2, phs1pgm1, and phs1isa3 of Arabidopsis thaliana. We observed different effects on carbon partitioning in these double mutants. Carbon allocation between starch and sucrose in different double mutants varied with respect to time and light conditions, with significant overall changes in phs1dpe1. Furthermore, we uncovered a potential time-specific function of PHS1 in maltodextrin metabolism. Changes in maltodextrin turnover exerted effects on the starch granule number and size in the double mutants, especially phs1dpe1. We further assessed the double mutants in terms of photosynthetic efficiency and starch parameters, such as internal structure and morphology, in detail. We found that the different photosynthetic parameters in pgm1 and its corresponding double mutant were affected relative to the wild type and phs1. However, other double mutants were not impaired in terms of photosynthetic efficiency despite alterations in their maltooligosaccharide levels.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2222-2238"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Earthworms and arbuscular mycorrhizal fungi improve salt tolerance in maize through symplastic pathways. 蚯蚓和丛枝菌根真菌通过共塑途径提高玉米的耐盐性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf057

Binglei Wang, Mingxuan Xiao, Jia Cao, Chong Wang

引用次数: 0

Sweet control: intracellular sorting of glycoproteins in plants. 甜控制：植物中糖蛋白的细胞内分选。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf095

Wencai Qi, Bingqi Zhang, Jiakai Yue, Liang Zhang

引用次数: 0

A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation. 毛杨叶芽中二氢查尔酮特异性o -甲基转移酶与芽树脂形成有关。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf020

Eerik-Mikael Piirtola, Dawei Ma, Jürgen Ehlting, C Peter Constabel

{"title":"A dihydrochalcone-specific O-methyltransferase from leaf buds of Populus trichocarpa implicated in bud resin formation.","authors":"Eerik-Mikael Piirtola, Dawei Ma, Jürgen Ehlting, C Peter Constabel","doi":"10.1093/jxb/eraf020","DOIUrl":"10.1093/jxb/eraf020","url":null,"abstract":"Production of secreted leaf bud resin is a mechanism for temperate trees to protect dormant leaf buds against frost damage, dehydration, and insect herbivory. Bud resins contain a wide variety of special metabolites including terpenoids, benzenoids, and phenolics. The leaf bud resins of Populus trichocarpa and P. balsamifera contain high concentrations of O-methylated dihydrochalcones, but the enzymes for methylating these compounds remain enigmatic. We used transcriptomics and differential gene expression analyses to identify a gene encoding a P. trichocarpa dihydrochalcone-specific O-methyltransferase, which we named PtDOMT1. Detailed enzymatic analyses demonstrated PtDOMT1 to be a highly selective and regiospecific O-methyltransferase which methylates 4 and 4' positions of dihydrochalcones using S-adenosyl-l-methionine as a methyl donor. PtDOMT1 did not methylate any other flavonoid or phenolic substrate tested. These findings establish the final step in the biosynthesis of O-methylated dihydrochalcones in poplar and represent the first molecular analysis of leaf bud resin biosynthesis in plants.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2129-2143"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LIPID RICH 1 modulates allocation of carbon between starch and triacylglycerol in Arabidopsis leaves. 脂质富1调节拟南芥叶片淀粉和甘油三酯之间的碳分配。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf048

Mebae Yamaguchi, Shuji Shigenobu, Katsushi Yamaguchi, Yasuhiro Higashi, Yozo Okazaki, Kazuki Saito, Emi Mishiro-Sato, Keiko Kano, Ryosuke Sugiyama, Mami Yamazaki, Shigeo S Sugano, Shuichi Fukuyoshi, Haruko Ueda, Ikuko Hara-Nishimura, Takashi L Shimada

{"title":"LIPID RICH 1 modulates allocation of carbon between starch and triacylglycerol in Arabidopsis leaves.","authors":"Mebae Yamaguchi, Shuji Shigenobu, Katsushi Yamaguchi, Yasuhiro Higashi, Yozo Okazaki, Kazuki Saito, Emi Mishiro-Sato, Keiko Kano, Ryosuke Sugiyama, Mami Yamazaki, Shigeo S Sugano, Shuichi Fukuyoshi, Haruko Ueda, Ikuko Hara-Nishimura, Takashi L Shimada","doi":"10.1093/jxb/eraf048","DOIUrl":"10.1093/jxb/eraf048","url":null,"abstract":"Plants accumulate starch and triacylglycerols (TAGs) as carbon sources. Leaves primarily store starch in chloroplasts, with some TAGs stored in lipid droplets, but how carbon resource allocation is regulated in leaves during cellular metabolism is largely unknown. Using a forward genetics approach, we isolated an Arabidopsis thaliana mutant with more lipid droplets in its leaves than the wild type, named lipid rich 1 (liri1). The overaccumulation of lipid droplets was caused by loss of function in the causal gene, encoding an uncharacterized protein. TAG levels were 5-fold higher and starch levels 2-fold lower in the leaves of liri1 than in those of the wild type. LIRI1 localized to the chloroplasts, and contents of chloroplast membrane lipids were 20% higher in liri1 leaves than in wild-type leaves. Co-immunoprecipitation assays revealed that LIRI1 interacts with ACETYL-COENZYME A CARBOXYLASE CARBOXYLTRANSFERASE ALPHA SUBUNIT (an enzyme for fatty acid biosynthesis) and STARCH SYNTHASE 4 (an enzyme for starch biosynthesis). In isotope tracer experiments using [1-13C]sodium acetate, more 13C was incorporated into TAGs in liri1 leaves than in wild-type leaves. Moreover, liri1 plants showed growth defects and irregular chloroplasts. These results indicate that LIRI1 affects the carbon trade-off to inhibit lipid production in leaves.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2144-2161"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway. 大麦叶片-叶柄基因Uniculme4对植株结构和茉莉酸途径的多效性影响

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf068

Elahe Tavakol, Vahid Shariati, Irene Maria Fontana, Marta Binaghi, Hagen Stellmach, Bettina Hause, Gerit Bethke, Hatice Bilgic, Jayanand Boddu, Ron Okagaki, Shane Heinen, Gary J Muehlbauer, Laura Rossini

{"title":"Pleiotropic effects of barley BLADE-ON-PETIOLE gene Uniculme4 on plant architecture and the jasmonic acid pathway.","authors":"Elahe Tavakol, Vahid Shariati, Irene Maria Fontana, Marta Binaghi, Hagen Stellmach, Bettina Hause, Gerit Bethke, Hatice Bilgic, Jayanand Boddu, Ron Okagaki, Shane Heinen, Gary J Muehlbauer, Laura Rossini","doi":"10.1093/jxb/eraf068","DOIUrl":"10.1093/jxb/eraf068","url":null,"abstract":"Plant architecture is a key determinant of crop yield, and understanding the genetic basis of its regulation is crucial for crop improvement. BLADE-ON-PETIOLE (BOP) genes are known to play a fundamental role in shaping plant architecture across diverse species. In this study, we demonstrate pleiotropic effects of the barley BOP gene Uniculme4 (Cul4) on various aspects of plant architecture, including plant height, culm diameter, and grain traits. Accordingly, Cul4 is broadly expressed in different tissues and developmental stages. Comparing transcriptome profiles of cul4 mutant and wild-type plants, we uncover a novel link between Cul4 and the jasmonic acid (JA) biosynthetic pathway. Our findings demonstrate that proper Cul4 function is required to repress JA biosynthesis, with cul4 mutants exhibiting increased levels of JA and its precursor 12-oxo-phytodienoic acid. Up-regulation of WRKY and bHLH transcription factors shows JA signalling is also impacted by Cul4. Additionally, our study sheds light on the role of Cul4 in flowering time regulation, potentially through its interaction with florigen-like genes. This research enhances our understanding of the mechanisms and pathways acting downstream of BOP genes.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2177-2191"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12116171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning of sulfur flow and sulfur seed metabolism in oilseed rape under sulfate-limited conditions. 硫酸盐限制条件下油菜硫流调控及硫粒代谢

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-05-27 DOI: 10.1093/jxb/eraf028

Philippe D'Hooghe, Stanislav Kopriva, Jean-Christophe Avice, Jacques Trouverie

{"title":"Tuning of sulfur flow and sulfur seed metabolism in oilseed rape under sulfate-limited conditions.","authors":"Philippe D'Hooghe, Stanislav Kopriva, Jean-Christophe Avice, Jacques Trouverie","doi":"10.1093/jxb/eraf028","DOIUrl":"10.1093/jxb/eraf028","url":null,"abstract":"The response of oilseed rape to sulfur (S) restriction usually consists of increasing the components of S utilization efficiency (absorption, assimilation, and remobilization) to provide S to seeds. However, source-sink relationships and S management in developing seeds under sulfate restriction are poorly understood. To address this, impacts of sulfate restrictions applied at 'visible bud' or 'start of pod filling' stages were studied in two genotypes, Aviso and Capitol. The two cultivars have similar seed yield, but Capitol has higher seed weight and lower number of seeds per plant under non-limited conditions. S flow at the whole-plant level (using [34S]sulfate labelling) and S metabolism changes [S-compounds, and ATP sulfurylase and adenosine 5'-phosphosulfate reductase (APR) activities] were studied during seed development. Seed yield, protein quality, and accumulation of S metabolites were affected by sulfate restriction less and later in Aviso than in Capitol. This was related to higher S uptake and stronger remobilization of S from vegetative organs to seeds during early seed development in response to sulfate restriction. A higher seed APR activity was observed for Capitol in response to sulfate limitation, suggesting that APR is not limiting for sulfate assimilation and that seed S metabolism is principally devoted to S-amino acids and protein synthesis.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"2278-2296"},"PeriodicalIF":5.6,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0