Journal of Experimental Botany最新文献_第6页

Adjustments of plant primary metabolism in the face of climate change. 面对气候变化，植物初级新陈代谢的调整。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-12 DOI: 10.1093/jxb/eraf116

Mustafa Bulut, Esra Karakas, Alisdair R Fernie

{"title":"Adjustments of plant primary metabolism in the face of climate change.","authors":"Mustafa Bulut, Esra Karakas, Alisdair R Fernie","doi":"10.1093/jxb/eraf116","DOIUrl":"https://doi.org/10.1093/jxb/eraf116","url":null,"abstract":"Plant metabolism is profoundly affected by various abiotic stresses. Consequently, plants must reconfigure their metabolic networks to sustain homeostasis while synthesizing compounds that mitigate stress. This aspect, with the current intensified climate impact results in more frequent abiotic stresses on a global scale. Advances in metabolomics and systems biology in the last decades have enabled both a comprehensive overview and a detailed analysis of key components involved in the plant metabolic response to abiotic stresses. This review addresses metabolic responses to altered atmospheric CO2 and O3, water deficit, temperature extremes, light intensity fluctuations including the importance of UV-B, ionic imbalance, and oxidative stress predicted to be caused by climate change, long-term shifts in temperatures and weather patterns. It also assesses both the commonalities and specificities of metabolic responses to diverse abiotic stresses, drawing on data from the literature. Classical stress-related metabolites such as proline, and polyamines are revisited, with an emphasis on the critical role of branched-chain amino acid metabolism under stress conditions. Finally, where possible, mechanistic insights into the regulation of metabolic processes and further outlook on combinatory stresses are discussed.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Coloration and Epicuticular Wax Properties of the Distinctive Glaucous Leaves of Encephalartos horridus. 角叉菜独特釉叶的结构颜色和表皮蜡特性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-12 DOI: 10.1093/jxb/eraf115

Takashi Nobusawa, Takashi Okamoto, Michiharu Nakano, Makoto Kusaba

{"title":"Structural Coloration and Epicuticular Wax Properties of the Distinctive Glaucous Leaves of Encephalartos horridus.","authors":"Takashi Nobusawa, Takashi Okamoto, Michiharu Nakano, Makoto Kusaba","doi":"10.1093/jxb/eraf115","DOIUrl":"https://doi.org/10.1093/jxb/eraf115","url":null,"abstract":"The leaves of the cycad Encephalartos horridus exhibit a conspicuous glaucous appearance, attributed to the presence of epicuticular wax. However, the molecular and optical bases of this coloration have not been scientifically explained. In this study, we conducted a detailed analysis of the epicuticular wax composition, combined with RNA-Seq and de novo transcriptome assembly, to uncover the molecular mechanisms underlying this phenomenon. Additionally, Monte Carlo multi-layer (MCML) simulations were performed to model light interactions and explore the structural coloration generated by the epicuticular wax crystals. The wax was found to be predominantly composed of nonacosan-10-ol, forming tubular crystals that enhance reflectance in the long-wavelength UV to blue light range. However, the microstructure alone is not sufficient to produce the glaucous appearance; it arises from the interplay between the wax crystals and the underlying dark tissues rich in chlorophyll. These findings provide insights into the evolutionary adaptations of cycads to UV exposure and contribute to a broader understanding of plant surface lipid biosynthesis and structural coloration, with potential applications in biomimetic material design.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycorrhizae and grapevines: the known unknowns of their interaction for wine growers´ challenges. 菌根与葡萄树：已知未知的相互作用给葡萄种植者带来的挑战。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-11 DOI: 10.1093/jxb/eraf081

Maider Velaz, Luis Gonzaga Santesteban, Nazareth Torres

{"title":"Mycorrhizae and grapevines: the known unknowns of their interaction for wine growers´ challenges.","authors":"Maider Velaz, Luis Gonzaga Santesteban, Nazareth Torres","doi":"10.1093/jxb/eraf081","DOIUrl":"https://doi.org/10.1093/jxb/eraf081","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) play an important role in grapevine production systems. However, little is known about how this relationship is achieved in the nursery and how soil management might modify it and its derived benefits. Here, we review the current knowledge on the establishment of grapevine-AMF relationships from the nursery to the field, the main factors that affect the effectiveness of the symbiosis, the potential role of AMF as biostimulants in grapevine production systems, and the future perspectives of their use in the current context of climate change. The process of establishing mycorrhizal symbiosis is complex, and the molecular dialogue between the plant roots and the fungus is still not yet fully understood. During vine plant production, rooting occurs in nurseries, where spontaneous symbiosis can be generated. The effectiveness of mycorrhizal symbiosis appears to depend not only on the identity of the fungus but also the diversity of the vine material and soil management. Finally, the use of AMF as biostimulants might be an effective strategy to face the new climatic scenario, but further research dealing with the application of AMF inocula and the protection of native cohorts should be conducted.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function of Cytochrome P450 CYP72A1182 in Metabolic Herbicide Resistance Evolution in Amaranthus palmeri Populations. 细胞色素P450 CYP72A1182在苋属植物代谢性抗除草剂进化中的作用

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-11 DOI: 10.1093/jxb/eraf114

Carlos Alberto Gonsiorkiewicz Rigon, Anita Küpper, Crystal Sparks, Jacob Montgomery, Falco Peter, Simon Schepp, Alejandro Perez-Jones, Patrick J Tranel, Roland Beffa, Franck E Dayan, Todd A Gaines

{"title":"Function of Cytochrome P450 CYP72A1182 in Metabolic Herbicide Resistance Evolution in Amaranthus palmeri Populations.","authors":"Carlos Alberto Gonsiorkiewicz Rigon, Anita Küpper, Crystal Sparks, Jacob Montgomery, Falco Peter, Simon Schepp, Alejandro Perez-Jones, Patrick J Tranel, Roland Beffa, Franck E Dayan, Todd A Gaines","doi":"10.1093/jxb/eraf114","DOIUrl":"https://doi.org/10.1093/jxb/eraf114","url":null,"abstract":"Evolution of metabolic herbicide resistance is a major issue for weed management. Few genes and regulatory mechanisms have been identified, particularly in dicotyledonous weed species. We identified putative causal genes and regulatory mechanism for tembotrione-resistance in Amaranthus palmeri. Cytochrome P450 candidate genes were identified through RNA-seq analysis. We validated their functions using heterologous expression in S. cerevisae. Promoters of the candidate P450 genes were analyzed. We performed QTL mapping to identify genomic regions associated with resistance. CYP72A1182 metabolized tembotrione in heterologous system. This gene had increased expression in other A. palmeri populations resistant to multiple herbicides, including tembotrione. Resistant plants exhibited polymorphisms in the promoter of CYP72A1182. We identified QTLs linked to herbicide resistance, including one on chromosome 4 approximately 3 Mb away from CYP72A1182. CYP72A1182 is likely involved in tembotrione resistance in A. palmeri. Increased expression of this gene could be due to cis-regulation in the promoter, as well as trans-regulation from transcription factors. Further studies are in progress to test this hypothesis. The elucidation of regulatory genes is crucial for developing innovative weed management approaches and target-based novel herbicide molecules.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming physiological trade-offs between flowering time and crop yield - strategies for a changing climate. 克服开花时间和作物产量之间的生理平衡——应对气候变化的策略。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-11 DOI: 10.1093/jxb/eraf110

Astrid Wingler, Soualihou Soualiou

{"title":"Overcoming physiological trade-offs between flowering time and crop yield - strategies for a changing climate.","authors":"Astrid Wingler, Soualihou Soualiou","doi":"10.1093/jxb/eraf110","DOIUrl":"https://doi.org/10.1093/jxb/eraf110","url":null,"abstract":"Early flowering of annual plants can lead to resource limitation owing to reduced uptake of nitrogen during the reproductive phase and declining foliar photosynthesis as a result of monocarpic senescence. Low availability of accumulated resources can therefore result in a trade-off between early flowering and reproductive fitness. However, green inflorescence organs (such as siliques, pods, bracts or awns) can make considerable contributions to photosynthetic carbon gain, and in some cases provide more carbon to seed formation than the leaves. Inflorescence photosynthesis may thereby overcome the flowering time trade-off. In addition to providing photosynthates, inflorescence organs can contribute to seed nitrogen through senescence-dependent nitrogen recycling. In annual crops, breeding has resulted in increased carbon allocation to the grain and higher harvest index, but in some cases, this had led to reduced grain protein content. We discuss different breeding targets to address carbon and nitrogen limitation, dependent on the climatic environment. For environments that are prone to drought, we propose a combination of early flowering with enhanced inflorescence photosynthesis, or, alternatively, delayed senescence (stay-green) associated with improved water balance. For optimized yield and grain protein content under favourable conditions, enhanced sink strength and extended nitrogen uptake are suggested as breeding targets.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Iron, cold iron, is the master of them all: iron crosstalk with zinc, copper, phosphorus and nitrogen homeostasis. 铁，冰冷的铁，是它们的主宰：铁与锌、铜、磷和氮的平衡相互影响。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf106

Andriele Wairich, Yugo Lima-Melo, Paloma Koprovski Menguer, Francieli Ortolan, Felipe Klein Ricachenevsky

{"title":"Iron, cold iron, is the master of them all: iron crosstalk with zinc, copper, phosphorus and nitrogen homeostasis.","authors":"Andriele Wairich, Yugo Lima-Melo, Paloma Koprovski Menguer, Francieli Ortolan, Felipe Klein Ricachenevsky","doi":"10.1093/jxb/eraf106","DOIUrl":"https://doi.org/10.1093/jxb/eraf106","url":null,"abstract":"The ionome is defined as the inorganic composition of an organism. In plants, the ionome has been shown to be integrated, as the concentration of elements affects one another, with complex regulatory mechanisms to keep nutrients, trace and toxic elements balanced. Iron (Fe) is an essential micronutrient that is necessary for photosynthesis, mitochondrial respiration, and redox metabolism, and has its concentrations in plant tissues finely regulated to avoid deficiency and excess stresses. It has been known that varying concentrations of Fe affect other components of the ionome, while variation in other elements's concentration also perturb iron homeostasis. Recently, molecular mechanisms that regulate the crosstalk of Fe homeostasis with that of zinc (Zn), copper (Cu), phosphorus (P) and nitrogen (N) have been uncovered. Here we review these regulatory circuits, demonstrating that the ionome should be balanced and that micronutrients are important for nutrient use efficiency and to avoid nutrient deficiency as well as excess. We focused mainly on model plant Arabidopsis thaliana and rice, for which mechanistic models have been proposed. Our review will help to integrate models to understand how plants balance the ionome.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building Climate-Resilient Crops: Genetic, Environmental, and Technological Strategies for Heat and Drought Stress Tolerance. 建设气候适应性作物：耐热和耐旱胁迫的遗传、环境和技术战略》（Genetic, Environmental, and Technological Strategies for Heat and Drought Stress Tolerance）。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf111

Karine Prado, Bethany L Holland, Brian McSpadden Gardener, Peter K Lundquist, James P Santiago, Robert VanBuren, Seung Y Rhee

{"title":"Building Climate-Resilient Crops: Genetic, Environmental, and Technological Strategies for Heat and Drought Stress Tolerance.","authors":"Karine Prado, Bethany L Holland, Brian McSpadden Gardener, Peter K Lundquist, James P Santiago, Robert VanBuren, Seung Y Rhee","doi":"10.1093/jxb/eraf111","DOIUrl":"https://doi.org/10.1093/jxb/eraf111","url":null,"abstract":"Global crop production faces increasing threats from the rise in frequency, duration, and intensity of drought and heat stress events due to climate change. Most staple food crops, including wheat, rice, soybean, and corn that provide over half of the world's caloric intake, are not well-adapted to withstand heat or drought. Efforts to breed or engineer stress-tolerant crops have had limited success due to the complexity of tolerance mechanisms and the variability of agricultural environments. Effective solutions require a shift towards fundamental research that incorporates realistic agricultural settings and focuses on practical outcomes for farmers. This review explores the genetic and environmental factors affecting heat and drought tolerance in major crops, examines the physiological and molecular mechanisms underlying these stress responses, and evaluates the limitations of current breeding programs and models. It also discusses emerging technologies and approaches that could enhance crop resilience, such as synthetic biology, advanced breeding techniques, and high-throughput phenotyping. Finally, this review emphasizes the need for interdisciplinary research and collaboration with stakeholders to translate fundamental research into practical agricultural solutions.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogen Sulfide and Protein Persulfidation in Plant Stress Signaling. 植物胁迫信号中的硫化氢和蛋白质过硫化。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf100

Mingjian Zhou, Yanjie Xie, Frank Van Breusegem, Jingjing Huang

{"title":"Hydrogen Sulfide and Protein Persulfidation in Plant Stress Signaling.","authors":"Mingjian Zhou, Yanjie Xie, Frank Van Breusegem, Jingjing Huang","doi":"10.1093/jxb/eraf100","DOIUrl":"https://doi.org/10.1093/jxb/eraf100","url":null,"abstract":"Hydrogen sulfide (H2S) is increasingly recognized as a crucial signaling molecule in plants, playing key roles in regulating physiological processes and enhancing stress tolerance. This review provides an updated summary of H2S signaling in plant stress responses, discussing its uptake from external environmental sources, its endogenous biosynthesis, and its broader functions in stress adaptation. We summarize the impact of H2S on plants under various stress conditions and review the mechanisms through which it mediates signaling functions, with a particular focus on H2S-mediated protein persulfidation. Additionally, we provide an overview of the current understanding of protein persulfidation in regulating physiological processes and stress responses in plants, offering both a general discussion of its effects under different stress conditions and specific examples to highlight its significance. Finally, we review recent proteomic studies on protein persulfidation in plants, comparing the identified persulfidated proteins across studies and highlighting shared biological processes and pathways. This review aims to consolidate the current understanding of H2S signaling and its roles mediated by protein persulfidation in plants, while also offering insights to inspire future research in this rapidly evolving field.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SnRK1 acts upstream of PRODUCTION OF ANTHOCYANIN PIGMENT1, contributing to fine-tuning of flavonoid biosynthesis during high-light acclimation. SnRK1作用于花青素色素生产的上游，有助于在强光驯化过程中对类黄酮的生物合成进行微调。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf098

Josephine Dieckmann, Ralf Stracke, Andreas S Richter

{"title":"SnRK1 acts upstream of PRODUCTION OF ANTHOCYANIN PIGMENT1, contributing to fine-tuning of flavonoid biosynthesis during high-light acclimation.","authors":"Josephine Dieckmann, Ralf Stracke, Andreas S Richter","doi":"10.1093/jxb/eraf098","DOIUrl":"https://doi.org/10.1093/jxb/eraf098","url":null,"abstract":"In land plants, one of the processes vital to cope with environmental changes is the accumulation of photoprotective flavonoids such as flavonols and anthocyanins. The inactivation of SUCROSE NON-FERMENTING1 RELATED PROTEIN KINASE1 (SnRK1), which acts in a chloroplast-derived sugar signalling pathway, permits the activation of flavonoid biosynthesis in high-light. The present study provides genetic evidence that SnRK1 acts upstream of PRODUCTION OF ANTHOCYANIN PIGMENT1 (PAP1), encoding a crucial transcription factor that activates the anthocyanin branch of flavonoid biosynthesis during high-light acclimation. A time-resolved expression analysis indicates a two-phasic suppression of MYB-LIKE2 (MYBL2), a repressor of anthocyanin production, involving SnRK1 inactivation for anthocyanin accumulation during prolonged high-light exposure. Furthermore, overexpression of PAP1 resulted in the marked suppression of MYB11, MYB12 and MYB111 and FLAVONOL SYNTHASE1, initiating the flavonol branch of the pathway. Analysis of a flavonoid-deficient chalcone synthase mutant overexpressing PAP1 suggests that regulation of flavonoid biosynthesis is PAP1-dependent but independent of flavonoid intermediates and end products. It is proposed that PAP1 suppresses the flavonol branch by an unknown mechanism, thereby promoting the consumption of carbon building blocks for anthocyanin production to permit the fine-tuning of the pathway.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A monomer-dimer switch modulates the activity of plant adenosine kinase. 一个单体-二聚体开关调节植物腺苷激酶的活性。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2025-03-10 DOI: 10.1093/jxb/eraf094

David Jaroslav Kopečný, Armelle Vigouroux, Jakub Bělíček, Martina Kopečná, Radka Končitíková, Jaroslava Friedecká, Václav Mik, Klára Supíková, Jan František Humplík, Marine Le Berre, Stephan Plancqueel, Miroslav Strnad, Klaus von Schwartzenberg, Ondřej Novák, Solange Moréra, David Kopečný

{"title":"A monomer-dimer switch modulates the activity of plant adenosine kinase.","authors":"David Jaroslav Kopečný, Armelle Vigouroux, Jakub Bělíček, Martina Kopečná, Radka Končitíková, Jaroslava Friedecká, Václav Mik, Klára Supíková, Jan František Humplík, Marine Le Berre, Stephan Plancqueel, Miroslav Strnad, Klaus von Schwartzenberg, Ondřej Novák, Solange Moréra, David Kopečný","doi":"10.1093/jxb/eraf094","DOIUrl":"https://doi.org/10.1093/jxb/eraf094","url":null,"abstract":"Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated the substrate preferences, oligomeric states and structures of ADKs from moss (Physcomitrella patens) and maize (Zea mays) alongside metabolomic and phenotypic analyses. We showed that dexamethasone-inducible ZmADK overexpressor lines in Arabidopsis can benefit from a higher number of lateral roots and larger root areas under nitrogen starvation. We discovered that maize and moss enzymes can form dimers upon increasing protein concentration, setting them apart from the monomeric human and protozoal ADKs. Structural and kinetic analyses revealed a catalytically inactive unique dimer. Within the dimer, both active sites are mutually blocked. The activity of moss ADKs, exhibiting a higher propensity to dimerize, was tenfold lower compared to maize ADKs. Two monomeric structures in a ternary complex highlight the characteristic transition from an open to a closed state upon substrate binding. This suggests that the oligomeric state switch can modulate the activity of moss ADKs and likely other plant ADKs. Moreover, dimer association represents a novel negative feedback mechanism, helping to maintain steady levels of adenosine and AMP.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":""},"PeriodicalIF":5.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0