Journal of Experimental Botany最新文献_第6页

Trichoderma gets by with a little help from Streptomyces: fungal-bacterial symbiosis in plant growth promotion.

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae439

Tessa E Reid, Miriam L Gifford

引用次数: 0

The role of lysophosphatidic acid acyltransferase 1 in reproductive growth of Arabidopsis thaliana. 溶血磷脂酸酰基转移酶 1 在拟南芥生殖生长中的作用

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae304

Niña Alyssa M Barroga, Van C Nguyen, Yuki Nakamura

引用次数: 0

Correction to: Scaling of leaf area with biomass in trees reconsidered: constant metabolically active sapwood volume per unit leaf area with height growth. 更正：重新考虑树木叶面积与生物量的比例关系：单位叶面积的代谢活性边材体积随高度增长保持不变。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae426

引用次数: 0

A negative feedback regulatory module comprising R3-MYB repressor MYBL2 and R2R3-MYB activator PAP1 fine-tunes high light-induced anthocyanin biosynthesis in Arabidopsis. 由 R3-MYB 抑制因子 MYBL2 和 R2R3-MYB 激活因子 PAP1 组成的负反馈调控模块对拟南芥中强光诱导的花青素生物合成进行了微调。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae399

Minghui Xing, Puman Xin, Yuetian Wang, Chunyan Han, Cangbao Lei, Weiyi Huang, Youpeng Zhang, Xiangyu Zhang, Kai Cheng, Xiao Zhang

{"title":"A negative feedback regulatory module comprising R3-MYB repressor MYBL2 and R2R3-MYB activator PAP1 fine-tunes high light-induced anthocyanin biosynthesis in Arabidopsis.","authors":"Minghui Xing, Puman Xin, Yuetian Wang, Chunyan Han, Cangbao Lei, Weiyi Huang, Youpeng Zhang, Xiangyu Zhang, Kai Cheng, Xiao Zhang","doi":"10.1093/jxb/erae399","DOIUrl":"10.1093/jxb/erae399","url":null,"abstract":"Anthocyanins, a group of flavonoids, play diverse roles in plant growth and environmental adaptation. The biosynthesis and accumulation of anthocyanin are regulated by environmental cues, such as high light. However, the precise mechanism underlying anthocyanin biosynthesis under high light conditions remains largely unclear. Here, we report that the R3-MYB repressor MYB-LIKE 2 (MYBL2) negatively regulates high light-induced anthocyanin biosynthesis in Arabidopsis by repressing two R2R3-MYB activators, PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1) and PAP2, which are core components of the MYB-bHLH-WD40 (MBW) complex. We found that MYBL2 interacts with PAP1/2 and reduces their transcriptional activation activities, thus disrupting the expression of key genes involved in anthocyanin biosynthesis, such as DIHYDROFLAVONOL 4-REDUCTASE (DFR) and TRANSPARENT TESTA 19 (TT19). Additionally, MYBL2 attenuates the transcriptional activation of PAP1 and its own expression, but not that of PAP2. Conversely, PAP1 collaborates with TRANSPARENT TESTA 8 (TT8), a bHLH member of the MBW complex, to activate MYBL2 transcription when excessive anthocyanins are accumulated. Taken together, our findings reveal a negative feedback regulatory module composed of MYBL2 and PAP1 that fine-tunes high light-induced anthocyanin biosynthesis through modulating MBW complex assembly.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7381-7400"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase variants identified via directed evolution. 通过定向进化发现的抗除草剂 HPPD 变异体。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae330

Hongjia Qian, Huazhong Shi

{"title":"Herbicide-resistant 4-hydroxyphenylpyruvate dioxygenase variants identified via directed evolution.","authors":"Hongjia Qian, Huazhong Shi","doi":"10.1093/jxb/erae330","DOIUrl":"10.1093/jxb/erae330","url":null,"abstract":"Herbicides play a crucial role in boosting crop yields, yet the emergence of herbicide-resistant weeds and the susceptibility of crops to herbicides have posed significant challenges to their efficacy. β-Triketone herbicides specifically target the enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD), which is essential for plant growth. Remarkably, few resistant weeds have been identified against these herbicides. In this study, we aimed to identify mutations within the cotton HPPD gene that confer resistance to mesotrione, a widely used triketone herbicide. Through the establishment of a high-throughput mutant screening system in Escherichia coli, we identified four single nucleotide changes leading to amino acid substitutions in HPPD, resulting in mesotrione resistance while preserving native enzymatic activity. Various combinations of these mutations displayed synergistic effects on herbicide resistance. Additionally, the HPPD variants were able to complement the Arabidopsis athppd mutant, indicating their retention of sufficient native activity for plant growth and development. Expression of these cotton HPPD variants in Arabidopsis resulted in heightened herbicide resistance. These findings offer critical insights into the target amino acids of HPPD for gene editing, paving the way for the development of herbicide-resistant cotton in the future.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7096-7106"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141855706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Local signal from Trichoderma afroharzianum T22 induces host transcriptome and endophytic microbiome leading to growth promotion in sorghum. 黄曲霉 T22 发出的局部信号可诱导宿主转录组和内生微生物组，从而促进高粱的生长。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae340

Ahmad H Kabir, Asha Thapa, Md Rokibul Hasan, Md Rasel Parvej

{"title":"Local signal from Trichoderma afroharzianum T22 induces host transcriptome and endophytic microbiome leading to growth promotion in sorghum.","authors":"Ahmad H Kabir, Asha Thapa, Md Rokibul Hasan, Md Rasel Parvej","doi":"10.1093/jxb/erae340","DOIUrl":"10.1093/jxb/erae340","url":null,"abstract":"Trichoderma, a highly abundant soil fungus, may benefit plants, yet it remains understudied in sorghum (Sorghum bicolor). In this study, sorghum plants were grown for 5 weeks in pots of soil with or without inoculation of T. afroharzianum T22. Inoculation with T. afroharzianum T22 significantly increased growth parameters and nutrient levels, demonstrating its beneficial role in sorghum. A split-root assay demonstrated that T. afroharzianum T22 is essential in both compartments of the pot for promoting plant growth, suggesting that local signals from this fungus drive symbiotic benefits in sorghum. RNA-seq analysis revealed that inoculation with T. afroharzianum T22 induced genes responsible for mineral transport (such as nitrate and aquaporin transporters), auxin response, sugar assimilation (hexokinase), and disease resistance (thaumatin) in sorghum roots. Microbial community analysis further unveiled the positive role of T. afroharzianum T22 in enriching Penicillium and Streptomyces while reducing disease-causing Fusarium in the roots. The microbial consortium, consisting of enriched microbiomes from bacterial and fungal communities, showed disrupted morphological features in plants inoculated with T. afroharzianum T22 in the absence of Streptomyces griseus. However, this disruption was not observed in the absence of Penicillium chrysogenum. These results indicate that S. griseus acts as a helper microbe in close association with T. afroharzianum T22 in the sorghum endosphere. This study provides the first comprehensive explanation of how T. afroharzianum T22 modulates host molecular determinants and endophytic helper microbes, thereby collectively promoting sorghum growth. These findings may facilitate the formulation of synthetic microbial inoculants dominated by T. afroharzianum T22 to enhance growth and stress resilience in sorghum and similar crops.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7107-7126"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated multiplex gene editing of gamma and omega gliadins: paving the way for gliadin-free wheat. CRISPR/Cas9 介导的γ 和ω 胶质蛋白多重基因编辑，为实现无胶质蛋白小麦铺平道路。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae376

Susana Sánchez-León, Miriam Marín-Sanz, María H Guzmán-López, Marta Gavilán-Camacho, Edurne Simón, Francisco Barro

{"title":"CRISPR/Cas9-mediated multiplex gene editing of gamma and omega gliadins: paving the way for gliadin-free wheat.","authors":"Susana Sánchez-León, Miriam Marín-Sanz, María H Guzmán-López, Marta Gavilán-Camacho, Edurne Simón, Francisco Barro","doi":"10.1093/jxb/erae376","DOIUrl":"10.1093/jxb/erae376","url":null,"abstract":"Wheat is a staple cereal in the human diet. Despite its significance, an increasing percentage of the population suffers adverse reactions to wheat, which are triggered by wheat gluten, particularly the gliadin fractions. In this study, we employed CRISPR/Cas [clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein] multiplexing to introduce targeted mutations into γ- and ω-gliadin genes of wheat, to produce lines deficient in one or both immunogenic gliadin fractions simultaneously. For this study, eight single guide RNAs (sgRNAs) were designed and combined into four plasmids to produce 59 modified wheat lines, of which 20 exhibited mutations in the target genes. Characterization of these lines through Sanger sequencing or next-generation sequencing revealed a complex pattern of InDels, including deletions spanning multiple sgRNAs. The mutations were transmitted to the offspring, and the analysis of homozygous derived lines by reverse-phase HPLC and monoclonal antibodies showed a 97.7% reduction in gluten content. Crossing these lines with other CRISPR/Cas lines deficient in the α-gliadins allowed multiple mutations to be combined. This work represents an important step forward in the use of CRISPR/Cas to develop gluten-free wheat.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7079-7095"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11630021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming weeds: breeding herbicide-resistant crops via directed evolution. 战胜杂草：通过定向进化培育抗除草剂作物。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae445

Liang Ma, Yan Guo

引用次数: 0

Differential warming at crown scale impacts walnut primary growth onset and secondary growth rate. 树冠尺度的升温差异影响核桃的初生和次生速度。

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae360

Nicolas Dusart, Bruno Moulia, Marc Saudreau, Christophe Serre, Guillaume Charrier, Félix P Hartmann

{"title":"Differential warming at crown scale impacts walnut primary growth onset and secondary growth rate.","authors":"Nicolas Dusart, Bruno Moulia, Marc Saudreau, Christophe Serre, Guillaume Charrier, Félix P Hartmann","doi":"10.1093/jxb/erae360","DOIUrl":"10.1093/jxb/erae360","url":null,"abstract":"Trees are exposed to significant spatio-temporal thermal variations, which can induce intra-crown discrepancies in the onset and dynamics of primary and secondary growth. In recent decades, an increase in late winter and early spring temperatures has been observed, potentially accelerating bud break, cambial activation, and their coordination. Intra-crown temperature heterogeneities could lead to asymmetric tree shapes unless there is a compensatory mechanism at the crown level. An original warming experiment was conducted on young Juglans regia trees in a greenhouse. The average temperature difference during the day between warmed and control parts from February to August was 4 °C. The warming treatment advanced the date of budbreak significantly, by up to 14 d. Warming did not alter secondary growth resumption but increased growth rates, leading to higher xylem cell production (by 2-fold) and to an increase in radial increment (+80% compared with control). Meristem resumptions were asynchronous without coordination in response to temperature. Buds on warmed branches began to swell 2 weeks prior to cambial division, which was 1 week earlier than on control branches. A difference in carbon and water remobilization at the end of bud ecodormancy was noted under warming. Overall, our results argue for a lack of compensatory mechanisms at the crown scale, which may lead to significant changes in tree architecture in response to intra-crown temperature heterogeneities.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7127-7144"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142119991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sterility of basal spikelets in wheat: predetermined fate or a matter of resources? 小麦基部小穗的不育性。命运注定还是资源问题？

IF 5.6 2区生物学

Journal of Experimental Botany Pub Date : 2024-12-04 DOI: 10.1093/jxb/erae373

Santiago Tamagno, Constanza S Carrera, Sofia I Marchese, Roxana Savin, Gustavo A Slafer

{"title":"Sterility of basal spikelets in wheat: predetermined fate or a matter of resources?","authors":"Santiago Tamagno, Constanza S Carrera, Sofia I Marchese, Roxana Savin, Gustavo A Slafer","doi":"10.1093/jxb/erae373","DOIUrl":"10.1093/jxb/erae373","url":null,"abstract":"Grains per spike in wheat (Triticum aestivum L.) are not uniformly distributed. Basal spikelets are typically sterile (or rudimentary) under common agronomic field conditions leading to the assumption that this is a constitutive trait. Yet, it is unknown whether high resource availability per plant could prevent floret mortality in basal spikelets. We present data from two field experiments assessing spike fertility in the four most basal spikelets of spikes from the main shoot and tillers. A collection of genotypes with different years of release and tillering habits were grown under contrasting sowing densities (agronomic densities versus isolated plants) to generate a condition of high assimilate supply to the spike. Under agronomic densities, the four most basal positions presented sterility in almost all cases in the main shoot spikes and without exception in the tiller spikes. When plants were isolated in each experiment, sterility in basal spikelets was absent and less frequent in spikes from the main shoot and tillers. Moreover, in the isolated condition, modern genotypes had a higher number of grains per spikelet than their older counterparts, whereas genotypes with low tillering habit showed the least response in spikelet fertility. These results indicate that while the predetermined lanceolate shape of spikes leads to the sterility of basal spikelets at high densities, this sterility can be reversed if assimilates become more available. Thus, sterility of basal spikelets in common agronomic conditions is not a constitutive condition of the spike.","PeriodicalId":15820,"journal":{"name":"Journal of Experimental Botany","volume":" ","pages":"7160-7173"},"PeriodicalIF":5.6,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0